CN202519255U

CN202519255U - Cell culture bag

Info

Publication number: CN202519255U
Application number: CN 201220070519
Authority: CN
Inventors: 任峰; 韩光
Original assignee: Tianjin MD Pacific Technology Co Ltd
Current assignee: Virtue Pacific (Tianjin) biological Polytron Technologies Inc
Priority date: 2012-02-29
Filing date: 2012-02-29
Publication date: 2012-11-07
Anticipated expiration: 2022-02-28

Abstract

The utility model relates to a cell culture bag comprising a bag body and liquid feeding pipes. Four edges of the bag body are hotly sealed to form a sealed edge; three cylindrical liquid feeding pipes are fixedly arranged and uniformly distributed on the edge of the upper part of the bag body in an axial direction; the three liquid feeding pipes can be connected with a pipeline; and the end part of the liquid feeding pipes in the bag are axially provided with two symmetrical supporting rods. The cell culture bag disclosed by the utility model has a simple structure and is scientific and reasonable; a gas exchanging region in the bag body is large so as to be good for culturing cells; and more high-quality cells can be obtained and a plurality of operations including microscopic observation and the like can be directly carried out, so that the whole process is guaranteed to be sterile and the operation is convenient and rapid.

Description

Cell culture bags

Technical field

The utility model belongs to biomedical engineering and medical test technical field, relates to cell culture container, especially a kind of cell culture bags.

Background technology

All vitro culture of cell cultures general reference, its implication are meant in the living animal body takes out tissue, places in the specific body such as physiological environment in the analogue body under the condition, hatches cultivation, makes it existence and growth.Cell cultures work has been widely used in every field such as biology, medical science, new drug development at present.At present, the spectacle case bottling splendid attire substratum that adopt PET or PET6 material when medical and scientific research field carry out cell cultures more, and adopt the Gamma gamma ray sterilization, and levels of sterility (SAL) is greater than 10 ^-6Other Biological Detection standard of level, no cytotoxicity, no thermal source, this spectacle case bottling belongs to the disposable products of model of instant use once to turn it on.

But conventional special-purpose culturing bottle exists that the bottleneck opening is big, stopping property is poor, the problem that the probability that operator are infected is bigger; And; Space inner cell density limited in the bottle is bigger, is unfavorable for exchange of air, is unfavorable for the cultivation of cell; Take out operations such as cell experimentizes and all need open bottle cap, cause the stopping property of culture environment to be affected; When using the upgrowth situation of the inner cell of microscopic examination, if nutrient solution is more, bottled overflows easily, causes the chance of cells infected to increase greatly.

Summary of the invention

The purpose of the utility model is to overcome the weak point of prior art, and a kind of scientific and reasonable, simple in structure, easy to use, cell culture bags that culture effect is good is provided.

The utility model solves its technical problem and takes following technical scheme to realize:

A kind of cell culture bags is characterized in that: comprise bag and liquid-inlet pipe, sealing the margin is processed in said bag four limit heat seals, at the coaxial liquid-inlet pipe that is fixed with three round tube types that is uniformly distributed with of the sealing the margin on bag top, but the equal connecting pipeline of these three liquid-inlet pipes.

And said liquid-inlet pipe is at the inner coaxial two symmetric support bars that are shaped in end of bag.

And, be shaped on the protruding circle of ring seal in the outer end of liquid-inlet pipe, through the coaxial installation sealing of the protruding circle of ring seal end cap.

And the sealing the margin symmetry of the bottom of bag is shaped on pilot hole.

And, be connected flank with the coaxial symmetry of liquid-inlet pipe dual-side of bag sealing the margin junction is shaped on.

The advantage and the positively effect of the utility model are:

1, this cell culture bags is through bag and extraneous exchange of air, and gas exchange area is bigger, helps in limited space, carrying out a large amount of cell cultures, can obtain more how high-quality cell.

2, this cell culture bags can be applied to cell cultures, cell amplification, transgenic, continous pouring cultivation and antibody generation etc.; The material that culture bag is chosen is transparent pliable and tough; Be convenient at microscopically direct viewing cell state and supervision cell cultivation process; Can in this culture bag, carry out cell cultures, microscopic examination and freezing, guarantee omnidistance sterile state, and effectively reduce the probability of cell contamination and operator's infection.

3, the said bag of this cell culture bags adopts EVA (plastic, rubber) material hot synthetic type to process, and the EVA material is current material, i.e. Injecatable EVA Copolymer; Be called for short EVA, claim plastic, rubber again, be characterized in having good flexibility; Elasticity as the rubber, high temperature resistant, still have good pliability, the transparency and surface luster property at-50 ℃; Chemicalstability is good, and anti-aging and anti-ozone intensity is good, nontoxicity; And forming process property is good, and this material is applied to its advantage of culture bag to be had and can pass through O ₂And CO ₂, but waterproof, there are not biology, chemically reactive, but ultraviolet-transmitting, visible light and infrared rays, and guarantee that culture bag can keep toughness in liquid nitrogen, and carry out autoclaving at 200 ℃.

4, two support bars made in the inner liquid-inlet pipe end of bag of this culture bag can effectively reduce pressure, cushion, and guarantee that pipeline is unobstructed, avoid pushing and impacting the cell at sack place, play the effect of protection cell.

5, the utility model is simple in structure, scientific and reasonable, and the bag gas exchange area is big, helps the cultivation of cell, can obtain more how high-quality cell, and can directly carry out multiple operation such as microscopic examination, guarantee omnidistance aseptic, simple operation.

Description of drawings

Fig. 1 is the utility model structural front view;

Fig. 2 is the partial enlarged drawing of liquid-inlet pipe;

Fig. 3 is the axis sectional view of Fig. 2;

Fig. 4 is the right view of Fig. 2;

Fig. 5 is the axis sectional view of Fig. 4.

Embodiment

Below in conjunction with accompanying drawing and through specific embodiment the utility model is done further to detail, following examples are descriptive, are not determinate, can not limit the protection domain of the utility model with this.

A kind of cell culture bags comprises bag 1 and liquid-inlet pipe 2, and sealing the margin 5 is processed in said bag four limit heat seals; The coaxial liquid-inlet pipe that is fixed with three round tube types that is uniformly distributed with at the edge on bag top; But these three liquid-inlet pipes are connecting pipeline 4 all, can be used for joint detection instrument, exhaust, draw samples and supplemental medium, and said liquid-inlet pipe is at the inner coaxial two symmetric support bars 9 that are shaped in end of bag; Can effectively avoid the bag shutoff liquid-inlet pipe that is squeezed, guarantee that liquid-inlet pipe is unimpeded.

In order to seal liquid-inlet pipe, be shaped on the protruding circle 7 of ring seal in the outer end of liquid-inlet pipe, through the coaxial installation sealing of the protruding circle of ring seal end cap 3.

For the ease of the hanging up and using of culture bag, the sealing the margin symmetry of the bottom of bag is shaped on pilot hole 6.

In order to strengthen the stopping property of liquid-inlet pipe and bag, be connected flank 8 with the coaxial symmetry of liquid-inlet pipe dual-side of bag sealing the margin junction is shaped on.

Checking through comparison test obtains conclusion:

Use culturing bottle and this culture bag culturing human lymphocyte respectively, the cell source is healthy subjects PBMC (normal human's PMNC).OKT3 stimulated in 0-4 days; 4-15 days enlarged culturing; Culturing bottle and culture bag are used A, B, C, four kinds of substratum of D respectively, are cultivating 15 days mensuration cell concns and are breeding multiple, and the result on average exceeds more than 20% than culturing bottle with culture bag cultured cells quantity.

Claims

1. cell culture bags, it is characterized in that: comprise bag and liquid-inlet pipe, sealing the margin is processed in said bag four limit heat seals, at the coaxial liquid-inlet pipe that is fixed with three round tube types that is uniformly distributed with of the sealing the margin on bag top, but the equal connecting pipeline of these three liquid-inlet pipes.

2. cell culture bags according to claim 1 is characterized in that: said liquid-inlet pipe is at the inner coaxial two symmetric support bars that are shaped in end of bag.

3. cell culture bags according to claim 1 is characterized in that: be shaped on the protruding circle of ring seal in the outer end of liquid-inlet pipe, through the coaxial installation sealing of the protruding circle of ring seal end cap.

4. cell culture bags according to claim 1 is characterized in that: the sealing the margin symmetry of the bottom of bag is shaped on pilot hole.

5. cell culture bags according to claim 1 is characterized in that: be connected flank with the coaxial symmetry of liquid-inlet pipe dual-side of bag sealing the margin junction is shaped on.