CN212388030U - Microbial cultivation device - Google Patents

Microbial cultivation device Download PDF

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Publication number
CN212388030U
CN212388030U CN202020191130.9U CN202020191130U CN212388030U CN 212388030 U CN212388030 U CN 212388030U CN 202020191130 U CN202020191130 U CN 202020191130U CN 212388030 U CN212388030 U CN 212388030U
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microorganism
incubator
needle
culture dish
culture
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CN202020191130.9U
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Chinese (zh)
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彭筱荔
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Rejuven Dermaceutical Co ltd
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Rejuven Dermaceutical Co ltd
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Abstract

The utility model discloses a microorganism culture device, which comprises an incubator, a base and a culture dish, wherein the inner side of the incubator is provided with an ultraviolet germicidal lamp, and the middle part of the incubator is provided with a microorganism planting needle; the middle part of the incubator is provided with a rubber sealing ring, the middle part of the rubber sealing ring is tightly matched with a microorganism planting needle, the upper end of the microorganism planting needle is provided with a rubber ball, the right side of the middle part of the microorganism planting needle is provided with a feed pipe, the middle part of the microorganism planting needle is provided with a first valve, and the middle part of the feed pipe is provided with a second valve; the utility model discloses in directly entering into the culture dish with outside microorganism through inlet pipe, rubber ball and microorganism planting needle, easy operation mode, safe swift, avoid inoculating the in-process of culture dish with the microorganism to be contaminated, adjust the position of microorganism planting needle through operating gloves, can inoculate different microorganism bacterial in the culture dish region of difference to can reduce the dependence to aseptic room, promote the practicality.

Description

Microbial cultivation device
Technical Field
The utility model relates to a biological assay field, in particular to microorganism culture apparatus.
Background
Microorganism culture, which refers to the rapid growth and propagation of some (species) microorganisms by means of artificially prepared culture media and artificially created culture conditions, is called microorganism culture, and can be divided into pure culture and mixed culture, wherein the former refers to the culture and utilization of purified single strains; the latter means that microorganisms in mixed strains or natural samples (such as soil) are cultured, and then the diversity and the quantity of the microorganisms in the soil can be estimated to a certain extent according to the types and the quantity of the microorganisms growing on the culture medium. The culture medium is needed for culturing the microorganisms, the specific culture medium is prepared according to different types and life habits of the microorganisms, the key point of successful culture of the microorganisms is aseptic operation, and if culture instruments and the culture medium cannot be sterilized completely and mixed bacteria pollution is easy to fail in the culture process.
However, although the conventional microbial cultivation basically performs sterilization and inactivation treatment on a culture tool and a culture medium, the culture tool and the culture medium are easily contaminated again in the process of inoculating strains, and most of the conventional microbial cultivation can only be operated in a sterile room, but the sterile room is not popular, so that the cultivation of microorganisms is very inconvenient, and therefore, a microbial cultivation device is proposed to solve the problems.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a microbial cultivation device, which can effectively solve the problems in the background technology.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
a microbial culture device comprising: the incubator is a stainless steel cuboid frame, the front end of the incubator is made of a glass transparent material, the condition inside the incubator can be observed, the observation of the culture condition of microorganisms is facilitated, the base is arranged at the bottom of the incubator, the bottom of the base is made of a hollowed design, the bottom of the base is made of a rubber material, the good shock absorption effect is achieved, a motor is arranged at the bottom in the incubator, a rotating rod is coaxially and fixedly mounted at the upper end of the motor, the culture dish is mounted at the upper end of the rotating rod through a key groove in a clamped mode, the culture dish is a round container, a culture solution for containing the cultured microorganisms is contained in the culture dish, an ultraviolet sterilizing lamp is arranged at the inner side of the incubator and can generate ultraviolet rays for sterilizing and inactivating the culture solution inside the incubator and the culture dish, provide sterile environment for microbial cultivation, the incubator middle part is provided with the microorganism and plants the needle, the incubator middle part is provided with rubber seal, rubber seal middle part tight fit installs the microorganism and plants the needle, rubber seal is the silica gel material, has good ductility, and the microorganism is planted the needle and can carry out certain angle's rotation in rubber seal, microorganism is planted the needle upper end and is provided with the rubber bulb, the rubber bulb is the rubber ball structure, microorganism is planted needle middle part and is provided with the stopper, stopper fixed mounting is above rubber seal for to the restriction of microorganism planting needle position, avoids microorganism to plant the needle and scratch inside the incubator, microorganism is planted needle middle part right side and is provided with the inlet pipe, the inlet pipe is used for the inoculation of microorganism to use, microorganism is planted needle middle part and is provided with first valve, the device is used for controlling the opening and closing of the lower half section of the microorganism planting needle, the second valve is arranged in the middle of the feeding pipe and is used for controlling the opening and closing of the right section of the feeding pipe, when microorganisms need to be inoculated, the first valve is firstly closed, the second valve is opened, negative pressure is formed in the feeding pipe after air in the rubber ball is exhausted, the right end of the feeding pipe is communicated with the corresponding microorganism needing to be cultured, bacterial liquid enters the material storage frame through the feeding pipe through the negative pressure in the feeding pipe, then the first valve is opened, the second valve is closed, the rubber ball is extruded to enable the bacterial liquid in the material storage frame to enter the corresponding area in the culture dish through the microorganism planting needle, the device can enable external microorganisms to directly enter the culture dish through the feeding pipe, the rubber ball and the microorganism planting needle, the operation is simple, the mode is safe and fast, and pollution is avoided in the process of inoculating the microorganisms to the culture dish, the stability of the experiment is improved.
Preferably, incubator upper end left and right sides symmetry is provided with the sealing ring, the sealing ring lower extreme is provided with operating glove, operating glove adopts silica gel material, has good leakproofness, will operate glove fixed mounting inside the incubator through the sealing ring, guarantees the inside good sterile environment of incubator, adjusts the position of microorganism planting needle through operating glove, can inoculate the different culture dish region with the microbial species of difference to can reduce the reliance to aseptic room, promote the practicality.
Preferably, the upper end of the microorganism planting needle is provided with a material storage frame for storing microorganism liquid.
Preferably, the microorganism planting needle is a hollow tube made of glass material.
Preferably, be provided with the baffle in the culture dish, divide into a plurality of groups structures through the space of baffle in with the culture dish, can carry out the cultivation of multiunit microorganism simultaneously for do the cultivation contrast experiment, promote the efficiency of experiment.
Preferably, the bottom top of the incubator evenly is provided with the rotation groove, rotates the inslot roll and installs the roll pearl, the contact of roll pearl upper end and culture dish bottom promotes the stability of support, the stability of promotion structure for the culture dish through rolling the pearl and providing the support.
Compared with the prior art, the utility model discloses following beneficial effect has:
firstly, culture solution is injected into a culture dish in an incubator, the incubator is sealed and then is subjected to aseptic treatment by opening an ultraviolet germicidal lamp, when microorganisms need to be inoculated, a first valve is closed, a second valve is opened, air in a rubber ball is exhausted, negative pressure is formed in a feeding pipe, the right end of the feeding pipe is communicated with the corresponding microorganism needing to be cultured, bacteria liquid enters a material storage frame through the feeding pipe through the negative pressure in the feeding pipe, then the first valve is opened, the second valve is closed, the rubber ball is extruded to enable the bacteria liquid in the material storage frame to enter a corresponding area in the culture dish through a microorganism planting needle, external microorganisms can directly enter the culture dish through the feeding pipe, the rubber ball and the microorganism planting needle, the operation is simple, safety and rapidness are realized, and the microorganisms are prevented from being polluted in the process of inoculating the microorganisms into the culture dish, promote the stability of experiment, adjust the position of microorganism planting needle through operating gloves, can inoculate different microorganism strains to different culture dishes in regional to can reduce the dependence to aseptic room, promote the practicality.
Drawings
FIG. 1 is a view showing an overall structure of a microorganism culture apparatus according to the present invention;
FIG. 2 is a view showing the structure of the microorganism cultivating needle of the microorganism cultivating device of the present invention.
In the figure: 1. an incubator; 2. a base; 3. a motor; 4. rotating the rod; 5. a culture dish; 6. rolling the beads; 7. a partition plate; 8. an ultraviolet germicidal lamp; 9. a seal ring; 10. operating gloves; 11. a microorganism planting needle; 12. a rubber ball; 13. a rubber seal ring; 14. a limiting block; 15. a material storage frame; 16. a first valve; 17. a feed pipe; 18. a second valve.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
In which like parts are designated by like reference numerals. It should be noted that the terms "front," "back," "left," "right," "upper" and "lower" used in the following description refer to directions in the drawings, and the terms "bottom" and "top," "inner" and "outer" refer to directions toward and away from, respectively, the geometric center of a particular component.
Referring to fig. 1 and 2, a microorganism culture apparatus includes: the culture box comprises a culture box 1, a base 2 and a culture dish 5, wherein the culture box 1 is a stainless steel cuboid frame, the front end of the culture box 1 is made of a glass transparent material, the condition inside the culture box 1 can be observed, the condition of culturing microorganisms can be observed conveniently, the base 2 is arranged at the bottom of the culture box 1, the bottom of the base 2 is made of a hollowed design, the bottom of the base 2 is made of a rubber material, the culture box has a good cushioning effect, a motor 3 is arranged at the bottom of the culture box 1, a rotating rod 4 is coaxially and fixedly arranged at the upper end of the motor 3, the culture dish 5 is arranged at the upper end of the rotating rod 4 through a key groove in a clamping manner, the culture dish 5 is a circular container and is used for containing culture solution for culturing the microorganisms, a partition plate 7 is arranged in the culture dish 5, the space in the culture dish 5 is divided into a plurality of groups through the partition plate 7, the culture contrast experiment device is used for performing a culture contrast experiment and improving the efficiency of the experiment, a rotating groove is uniformly arranged above the bottom of the incubator 1, a rolling bead 6 is arranged in the rotating groove in a rolling manner, the upper end of the rolling bead 6 is in contact with the bottom of a culture dish 5, the culture dish 5 is supported by the rolling bead 6, the stability of the support is improved, and the stability of the structure is improved;
the utility model discloses a microorganism cultivation device, including incubator 1, ultraviolet germicidal lamp 8, for can take place the ultraviolet ray and be used for disinfecting the inactivation to the culture solution of incubator 1 inside and culture dish 5 inside, provide sterile environment for the microbial cultivation, incubator 1 middle part is provided with microorganism planting needle 11, as shown in figure 2, incubator 1 middle part is provided with rubber seal 13, microorganism planting needle 11 is installed to rubber seal 13 middle part tight fit, microorganism planting needle 11 is the hollow tubular product of glass material, rubber seal 13 is the silica gel material, has good ductility, and microorganism planting needle 11 can carry out certain angle's rotation in rubber seal 13, microorganism planting needle 11 upper end is provided with rubber bulb 12, rubber bulb 12 is the globular structure of rubber, microorganism planting needle 11 upper end is provided with deposits frame 15, the microorganism planting needle is used for storing microorganism liquid, a limiting block 14 is arranged in the middle of the microorganism planting needle 11, the limiting block 14 is fixedly installed above the rubber sealing ring 13 and used for limiting the position of the microorganism planting needle 11 and preventing the microorganism planting needle 11 from being scratched into the incubator 1, a feeding pipe 17 is arranged on the right side of the middle of the microorganism planting needle 11 and used for inoculating microorganisms, a first valve 16 is arranged in the middle of the microorganism planting needle 11 and used for controlling the opening and closing of the lower half section of the microorganism planting needle 11, a second valve 18 is arranged in the middle of the feeding pipe 17 and used for controlling the opening and closing of the right section of the feeding pipe 17, when the microorganism is required to be inoculated, the first valve 16 is firstly closed, the second valve 18 is opened, after air in the rubber ball 12 is discharged, negative pressure is formed in the feeding pipe 17, and the right end of the feeding pipe 17 is communicated with the corresponding microorganism to be cultured, through the negative pressure in the feeding pipe 17, the bacterial liquid enters the material storage frame 15 through the feeding pipe 17, then the first valve 16 is opened, the second valve 18 is closed, the rubber ball 12 is extruded to enable the bacterial liquid in the material storage frame 15 to enter a corresponding area in the culture dish 5 to be required through the microorganism planting needle 11, external microorganisms can directly enter the culture dish 5 through the feeding pipe 17, the rubber ball 12 and the microorganism planting needle 11 by the device, the operation is simple, the mode is safe and fast, the microorganism is prevented from being polluted in the process of inoculating the microorganisms to the culture dish, and the stability of an experiment is improved;
incubator 1 upper end left and right sides symmetry is provided with sealing ring 9, 9 lower extremes of sealing ring are provided with operation gloves 10, operation gloves 10 adopt silica gel material, have good leakproofness, through sealing ring 9 with operation gloves 10 fixed mounting inside incubator 1, guarantee the inside good sterile environment of incubator 1, adjust the position of microorganism planting needle 11 through operation gloves 10, can inoculate different microorganism strains in 5 regions of culture dish of difference to can reduce the reliance to aseptic room, promote the practicality.
The utility model discloses a theory of operation is: firstly, culture solution is injected into a culture dish 5 in an incubator 1, the incubator 1 is sealed and then an ultraviolet sterilization lamp 8 is turned on to carry out aseptic processing on the interior of the incubator 1, when microorganism inoculation is needed, a first valve 16 is closed, a second valve 18 is opened, air in a rubber ball 12 is exhausted, negative pressure is formed in a feeding pipe 17, the right end of the feeding pipe 17 is communicated with the corresponding microorganism needing to be cultured, bacteria liquid enters a material storage frame 15 through the feeding pipe 17 through the negative pressure in the feeding pipe 17, then the first valve 16 is opened, the second valve 18 is closed, the rubber ball 12 is squeezed to enable the bacteria liquid in the material storage frame 15 to enter a corresponding area in the culture dish 5 through a microorganism planting needle 11, and external microorganisms can directly enter the culture dish 5 through the feeding pipe 17, the rubber ball 12 and the microorganism planting needle 11, the operation is simple, safe swift, avoids inoculating the in-process of culture dish with the microorganism to be contaminated, promotes the stability of experiment, adjusts the position of microorganism planting needle 11 through operating gloves 10, can inoculate different microorganism bacterial to different culture dishes 5 in the region to can reduce the dependence to aseptic room, promote the practicality.
The basic principles and the main features of the invention and the advantages of the invention have been shown and described above. It will be understood by those skilled in the art that the present invention is not limited to the above embodiments, and that the foregoing embodiments and descriptions are provided only to illustrate the principles of the present invention without departing from the spirit and scope of the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims (7)

1. The utility model provides a microbial cultivation device, includes incubator (1), base (2) and culture dish (5), incubator (1) bottom is provided with base (2), the bottom is provided with motor (3) in incubator (1), the coaxial fixed mounting in motor (3) upper end has dwang (4), culture dish (5), its characterized in that are installed through the keyway joint in dwang (4) upper end: an ultraviolet germicidal lamp (8) is arranged on the inner side of the incubator (1), and a microorganism planting needle (11) is arranged in the middle of the incubator (1);
incubator (1) middle part is provided with rubber seal (13), rubber seal (13) middle part tight fit installs the microorganism and plants needle (11), the microorganism is planted needle (11) upper end and is provided with rubber ball (12), the microorganism is planted needle (11) middle part and is provided with stopper (14), stopper (14) fixed mounting is in rubber seal (13) top, the microorganism is planted needle (11) middle part right side and is provided with inlet pipe (17), the microorganism is planted needle (11) middle part and is provided with first valve (16), inlet pipe (17) middle part is provided with second valve (18).
2. A microorganism culture apparatus according to claim 1, wherein: incubator (1) upper end left and right sides symmetry is provided with sealing ring (9), sealing ring (9) lower extreme is provided with operating gloves (10), operating gloves (10) adopt the silica gel material, through sealing ring (9) with operating gloves (10) fixed mounting inside incubator (1).
3. A microorganism culture apparatus according to claim 1, wherein: the upper end of the microorganism planting needle (11) is provided with a material storage frame (15).
4. A microorganism culture apparatus according to claim 1, wherein: the microorganism planting needle (11) is a hollow pipe made of glass material.
5. A microorganism culture apparatus according to claim 1, wherein: and a partition plate (7) is arranged in the culture dish (5).
6. A microorganism culture apparatus according to claim 1, wherein: the incubator is characterized in that a rotating groove is uniformly formed above the bottom of the incubator (1), a rolling bead (6) is installed in the rotating groove in a rolling mode, and the upper end of the rolling bead (6) is in contact with the bottom of the culture dish (5).
7. A microorganism culture apparatus according to claim 1, wherein: the incubator (1) is a stainless steel cuboid frame, and the front end of the incubator (1) is made of a transparent glass material.
CN202020191130.9U 2020-02-21 2020-02-21 Microbial cultivation device Active CN212388030U (en)

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Application Number Priority Date Filing Date Title
CN202020191130.9U CN212388030U (en) 2020-02-21 2020-02-21 Microbial cultivation device

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Application Number Priority Date Filing Date Title
CN202020191130.9U CN212388030U (en) 2020-02-21 2020-02-21 Microbial cultivation device

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114107010A (en) * 2021-11-24 2022-03-01 宜宾职业技术学院 Device and method for automatically and synchronously coating culture dishes in multiple areas

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114107010A (en) * 2021-11-24 2022-03-01 宜宾职业技术学院 Device and method for automatically and synchronously coating culture dishes in multiple areas
CN114107010B (en) * 2021-11-24 2023-08-01 宜宾职业技术学院 Device and method for automatically and synchronously coating culture dish in multiple areas

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