CN214413771U - Negative pressure formula plant tissue culture explant degassing unit - Google Patents
Negative pressure formula plant tissue culture explant degassing unit Download PDFInfo
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- CN214413771U CN214413771U CN202120351515.1U CN202120351515U CN214413771U CN 214413771 U CN214413771 U CN 214413771U CN 202120351515 U CN202120351515 U CN 202120351515U CN 214413771 U CN214413771 U CN 214413771U
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Abstract
The utility model relates to a negative pressure type explant disinfection device for plant tissue culture, which comprises a horizontally arranged mounting plate and a negative pressure disinfection tank vertically fixed on the mounting plate; the lower end of the mounting plate is fixed with a plurality of vertical support rods; the negative pressure disinfection tank is of a barrel-shaped structure with an open upper end, and the open end is sealed by a sealing cover; the middle part of the sealing cover is embedded with an air inlet pipe; the bottom of the negative pressure disinfection tank is communicated with a liquid outlet pipe, and the liquid outlet pipe penetrates out of the mounting plate downwards; the side wall of one side of the negative pressure disinfection tank is communicated with a liquid inlet pipe, and the side wall of the other side of the negative pressure disinfection tank is communicated with a gas outlet pipe; a plurality of sealing lampshades are uniformly arranged on the inner circumferential surface of the negative pressure disinfection tank at intervals, and a plurality of ultraviolet sterilization lamps are arranged in each sealing lampshade; a funnel-shaped conical ramp is fixed on the bottom surface of the inner side of the negative pressure disinfection tank; the height of the conical ramp is gradually reduced from the periphery to the center, and the bottom of the center is communicated with the upper end of the liquid outlet pipe; a rack is arranged above the conical ramp.
Description
Technical Field
The utility model relates to a negative pressure formula plant tissue culture explant degassing unit belongs to plant tissue culture technical field.
Background
The whole technology of plant tissue culture is called plant tissue culture, and is a relatively mature plant rapid propagation technology, and an explant is an organ or a tissue segment which is used as an isolated culture material in the plant tissue culture, and when the explant is cultured, bacterial contamination has a great influence on the explant per se.
In addition to bacterial contamination in the environment, the explant itself also carries a large amount of bacteria, which rapidly multiply on the culture medium and ultimately affect the growth of plant cells, even kill plant cells, and therefore, sterilization of the explant during plant tissue culture is of great importance for killing microorganisms inside the explant.
Chinese utility model patent "a negative pressure formula plant tissue culture explant degassing unit" that grant bulletin number is CN205320819U, which comprises a tank body, the jar is equipped with the top cap on physically, the interior bottom of jar body is equipped with the sand net, still include with feed liquor pipe, intake pipe, drain pipe, exhaust tube of the inside intercommunication of the jar body, all be equipped with sealed lid on feed liquor pipe, intake pipe, drain pipe, the exhaust tube, the intake pipe all is equipped with millipore filtration with the inside of exhaust tube. This negative pressure formula plant tissue culture explant degassing unit easy operation adopts negative pressure formula disinfection mode, effectively organizes the abundant disinfection of explant to plant tissue, reduces the pollution that plant tissue culture process produced because explant self takes the fungus, reduces the pollution rate, improves economic benefits. However, the bottom of the tank body of the negative pressure type plant tissue culture explant disinfection device is horizontal, and even if a liquid outlet pipe is arranged, the horizontal bottom is not beneficial to discharging disinfectant; secondly, the disinfection liquid is only used for disinfecting the explant, the disinfection device body is not disinfected after being used, and the disinfection device body which is not disinfected may carry bacteria, which is not beneficial to the subsequent disinfection work of the explant.
SUMMERY OF THE UTILITY MODEL
In order to overcome the problem, the utility model provides a negative pressure formula plant tissue culture explant degassing unit, this negative pressure formula plant tissue culture explant degassing unit simple structure, design benefit, the toper ramp is favorable to the quick discharge of antiseptic solution, and the sterilamp then can disinfect to the self of disinfection jar, ensures plant tissue culture's in-process, the aseptic of disinfection jar.
The technical scheme of the utility model as follows:
a negative pressure type plant tissue culture explant disinfection device comprises a horizontally arranged mounting plate and a negative pressure disinfection tank vertically fixed on the mounting plate; a plurality of vertical support rods are fixed at the lower end of the mounting plate; the negative pressure disinfection tank is of a barrel-shaped structure with an open upper end, and the open end is sealed by a sealing cover; an air inlet pipe is embedded in the middle of the sealing cover; the bottom of the negative pressure disinfection tank is communicated with a liquid outlet pipe, and the liquid outlet pipe penetrates out of the mounting plate downwards; the side wall of one side of the negative pressure disinfection tank is communicated with a liquid inlet pipe, and the side wall of the other side of the negative pressure disinfection tank is communicated with a gas outlet pipe; a plurality of sealing lampshades are uniformly arranged on the inner circumferential surface of the negative pressure disinfection tank at intervals, and a plurality of ultraviolet sterilization lamps are arranged in each sealing lampshade; a funnel-shaped conical ramp is fixed on the bottom surface of the inner side of the negative pressure disinfection tank; the height of the conical ramp is gradually reduced from the periphery to the center, and the center bottom is communicated with the upper end of the liquid outlet pipe; a placing frame is arranged above the conical ramp.
Further, a negative pressure machine is fixed on the outer side wall of the negative pressure disinfection tank; the tail end of the air outlet pipe is communicated with the air inlet end of the negative pressure machine.
Furthermore, a rubber sucker with a downward opening is fixed at the lower end of the supporting rod.
Further, the placing rack comprises a horizontal placing plate and a plurality of support legs vertically fixed at the lower end of the placing plate; a placing net disc is embedded in the upper end face of the placing disc; the support feet stand on the tapered ramp.
Furthermore, the number of the supporting rods is four, and the supporting rods are arranged at four corners of the lower end of the mounting plate in a rectangular array.
Further, the number of the sealing lampshades is four.
Furthermore, valves for controlling respective opening and closing are arranged on the air inlet pipe, the air outlet pipe, the liquid inlet pipe and the liquid outlet pipe.
Furthermore, the inner periphery of the air inlet pipe is provided with a filter screen.
The utility model discloses following beneficial effect has:
1. this negative pressure formula plant tissue culture explant degassing unit simple structure, design benefit, the toper ramp is favorable to the quick discharge of antiseptic solution, and the ultraviolet germicidal lamp then can disinfect to the self of disinfection jar, ensures plant tissue culture's in-process, the aseptic of disinfection jar.
2. This negative pressure formula plant tissue culture explant degassing unit through setting up negative pressure disinfection jar, sealed lid, outlet duct and negative pressure machine, can make the inside pressure of negative pressure disinfection jar reach the negative pressure level, through setting up intake pipe and nanometer filter screen, can reach and carry out filterable purpose to the air that gets into the negative pressure disinfection jar to reach the influence that the microorganism in the elimination air propagated to the explant.
3. This negative pressure formula plant tissue culture explant degassing unit, through setting up the feed liquor pipe, be linked together through feed liquor pipe and outside antiseptic solution storage tank, can add the inside of negative pressure disinfection jar with the antiseptic solution, reach the purpose of disinfecting to the explant, through setting up drain pipe and conical weeping ramp, can emit the negative pressure disinfection jar with the antiseptic solution that adds in the negative pressure disinfection jar, through setting up sealed lamp shade and sterilamp, can use at the negative pressure disinfection jar after, reach the inside purpose of disinfecting to the negative pressure disinfection jar.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention.
Fig. 2 is a half-section view of the utility model.
Fig. 3 is a schematic diagram of the internal structure of the present invention.
The reference numbers in the figures denote:
1. mounting a plate; 2. a support bar; 3. a rubber suction cup; 4. a negative pressure disinfection tank; 5. a conical ramp; 6. a liquid outlet pipe; 7. sealing the lampshade; 8. a liquid inlet pipe; 9. a sealing cover; 10. an air inlet pipe; 11. an air outlet pipe; 12. placing a rack; 13. placing a net disc; 14. an ultraviolet sterilizing lamp; 15. a negative pressure machine; 16. and (4) a filter screen.
Detailed Description
The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.
Referring to fig. 1-3, a negative pressure type plant tissue culture explant sterilization device comprises a horizontally arranged mounting plate 1 and a negative pressure sterilization tank 4 vertically fixed on the mounting plate 1; a plurality of vertical support rods 2 are fixed at the lower end of the mounting plate 1; the negative pressure disinfection tank 4 is of a barrel-shaped structure with an open upper end, and the open end is sealed by a sealing cover 9; an air inlet pipe 10 is embedded in the middle of the sealing cover 9; the bottom of the negative pressure disinfection tank 4 is communicated with a liquid outlet pipe 6, and the liquid outlet pipe 6 penetrates out of the mounting plate 1 downwards; the side wall of one side of the negative pressure disinfection tank 4 is communicated with a liquid inlet pipe 8, and the side wall of the other side is communicated with an air outlet pipe 11; a plurality of sealing lampshades 7 are uniformly arranged on the inner circumferential surface of the negative pressure disinfection tank 4 at intervals, and a plurality of ultraviolet sterilization lamps 14 are arranged in each sealing lampshade 7; a funnel-shaped conical ramp 5 is fixed on the bottom surface of the inner side of the negative pressure disinfection tank 4; the height of the conical ramp 5 is gradually reduced from the periphery to the center, and the bottom of the center is communicated with the upper end of the liquid outlet pipe 6; a rack 12 is placed above the conical ramp 5.
According to the above description, the negative pressure type plant tissue culture explant sterilization device mainly comprises a mounting plate 1, a negative pressure sterilization tank 4, a support rod 2, a sealing cover 9, an ultraviolet sterilization lamp 14, a conical ramp 5 and a placing rack 12. The mounting plate 1 mainly plays a role in fixing the negative pressure disinfection tank 4; the support rod 2 is mainly used for supporting the mounting plate 1; the sealing cover 9 is covered above the negative pressure disinfection tank 4, so that the effect of sealing the negative pressure disinfection tank 4 is achieved; the pressure in the negative pressure disinfection tank 4 can reach the negative pressure level through the negative pressure disinfection tank 4, the sealing cover 9 and the air outlet pipe 11; the liquid inlet pipe 8 is communicated with an external disinfectant storage tank and can add disinfectant into the negative pressure disinfection tank 4; the conical ramp 5 is of a funnel-shaped structure, and can conveniently and quickly discharge the disinfectant added into the negative pressure disinfection tank 4 through the liquid outlet pipe 6; through sealed lamp shade 7 and ultraviolet sterilamp 14, can reach the purpose of disinfecting certainly to the inside of negative pressure disinfection jar 4 after negative pressure disinfection jar 4 uses to the operator of being convenient for carries out subsequent plant tissue culture work smoothly.
Further, a negative pressure machine 15 is fixed on the outer side wall of the negative pressure disinfection tank 4; the tail end of the air outlet pipe 11 is communicated with the air inlet end of the negative pressure machine 15. The negative pressure machine 15 is started to enable the pressure inside the negative pressure disinfection tank 4 to reach the negative pressure level.
Further, a rubber sucker 3 with a downward opening is fixed at the lower end of the support rod 2. The rubber suction cup 3 can be adsorbed on the workbench, so that the stability of the negative pressure disinfection tank 4 is ensured.
Further, the placing frame 12 comprises a horizontal placing disc and a plurality of support legs vertically fixed at the lower end of the placing disc; a placing net disc 13 is embedded on the upper end face of the placing disc; the feet stand on the conical ramp 5.
Further, the number of the support rods 2 is four, and the support rods 2 are arranged at four corners of the lower end of the mounting plate 1 in a rectangular array.
Further, the number of the sealing lamp housings 7 is four.
Furthermore, valves for controlling respective opening and closing are arranged on the air inlet pipe 10, the air outlet pipe 11, the liquid inlet pipe 8 and the liquid outlet pipe 6.
Further, a filter screen 16 is provided on the inner periphery of the intake duct 10. Through setting up intake pipe 10 and filter screen 16, can reach the purpose of filtering the air that gets into negative pressure disinfection jar 4 to reach the influence of the microorganism in the elimination air to explant reproduction.
The above only is the embodiment of the present invention, not limiting the patent scope of the present invention, all the equivalent structures or equivalent processes that are used in the specification and the attached drawings or directly or indirectly applied to other related technical fields are included in the patent protection scope of the present invention.
Claims (8)
1. The utility model provides a negative pressure formula plant tissue culture explant degassing unit which characterized in that: comprises a horizontally arranged mounting plate (1) and a negative pressure disinfection tank (4) vertically fixed on the mounting plate (1); a plurality of vertical support rods (2) are fixed at the lower end of the mounting plate (1); the negative pressure disinfection tank (4) is of a barrel-shaped structure with an open upper end, and the open end is sealed by a sealing cover (9); an air inlet pipe (10) is embedded in the middle of the sealing cover (9); a liquid outlet pipe (6) is communicated with the bottom of the negative pressure disinfection tank (4), and the liquid outlet pipe (6) penetrates out of the mounting plate (1) downwards; a liquid inlet pipe (8) is communicated with the side wall of one side of the negative pressure disinfection tank (4), and a gas outlet pipe (11) is communicated with the side wall of the other side; a plurality of sealing lampshades (7) are uniformly arranged on the inner circumferential surface of the negative pressure disinfection tank (4) at intervals, and a plurality of ultraviolet sterilization lamps (14) are arranged in each sealing lampshade (7); a funnel-shaped conical ramp (5) is fixed on the bottom surface of the inner side of the negative pressure disinfection tank (4); the height of the conical ramp (5) is gradually reduced from the periphery to the center, and the center bottom is communicated with the upper end of the liquid outlet pipe (6); a placing frame (12) is arranged above the conical ramp (5).
2. The negative pressure type plant tissue culture explant disinfection device of claim 1, wherein: a negative pressure machine (15) is fixed on the outer side wall of the negative pressure disinfection tank (4); the tail end of the air outlet pipe (11) is communicated with the air inlet end of the negative pressure machine (15).
3. The negative pressure type plant tissue culture explant disinfection device of claim 1, wherein: and a rubber sucker (3) with a downward opening is fixed at the lower end of the supporting rod (2).
4. The negative pressure type plant tissue culture explant disinfection device of claim 1, wherein: the placing rack (12) comprises a horizontal placing tray and a plurality of support legs vertically fixed at the lower end of the placing tray; a placing net disc (13) is embedded on the upper end face of the placing disc; the legs stand on the conical ramp (5).
5. The negative pressure type plant tissue culture explant disinfection device of claim 1, wherein: the number of the support rods (2) is four, and the support rods (2) are arranged at four corners of the lower end of the mounting plate (1) in a rectangular array mode.
6. The negative pressure type plant tissue culture explant disinfection device of claim 1, wherein: the number of the sealing lampshades (7) is four.
7. The negative pressure type plant tissue culture explant disinfection device of claim 1, wherein: valves for controlling respective opening and closing are arranged on the air inlet pipe (10), the air outlet pipe (11), the liquid inlet pipe (8) and the liquid outlet pipe (6).
8. The negative pressure type plant tissue culture explant disinfection device of claim 1, wherein: the inner periphery of the air inlet pipe (10) is provided with a filter screen (16).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120351515.1U CN214413771U (en) | 2021-02-08 | 2021-02-08 | Negative pressure formula plant tissue culture explant degassing unit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120351515.1U CN214413771U (en) | 2021-02-08 | 2021-02-08 | Negative pressure formula plant tissue culture explant degassing unit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214413771U true CN214413771U (en) | 2021-10-19 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120351515.1U Active CN214413771U (en) | 2021-02-08 | 2021-02-08 | Negative pressure formula plant tissue culture explant degassing unit |
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| Country | Link |
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| CN (1) | CN214413771U (en) |
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2021
- 2021-02-08 CN CN202120351515.1U patent/CN214413771U/en active Active
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