CN104762198A - Special inoculation frame for liquid cell culture - Google Patents
Special inoculation frame for liquid cell culture Download PDFInfo
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- CN104762198A CN104762198A CN201510180645.2A CN201510180645A CN104762198A CN 104762198 A CN104762198 A CN 104762198A CN 201510180645 A CN201510180645 A CN 201510180645A CN 104762198 A CN104762198 A CN 104762198A
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- China
- Prior art keywords
- pad
- liquid cell
- base plate
- cell according
- inoculation frame
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
Abstract
The invention relates to microbial culture and in particular relates to a special inoculation frame special for liquid cell culture. The inoculation frame comprises a base plate and a supporting plate of which the bottom is in running fit with the base plate, wherein a baffle plate is arranged at the rear part of the upper surface of the supporting plate, a bottleneck fixing mechanism is arranged at the front part of the upper surface of the supporting plate, and an angle adjusting device is arranged between the supporting plate and the base plate. The inoculation frame provided by the invention can be used for solving the technical problems of low inoculation speed, high operation strength, high contamination probability and the like of the prior art, and has the advantages of being stable in supporting structure, suitable for clamping fixation of triangular flasks with different specifications, high in inoculation speed, high in effect, saving in labor and the like.
Description
Technical field
The present invention relates to microorganism culturing, refer to that a kind of liquid cell cultivates special inoculation frame especially.
Background technology
Microbial inoculant is the elementary operation technology in microbiological research, all need microorganism to be forwarded in the substratum of another fresh sterilizing in microbiology test and fermentative production, make its growth and breeding by cultivation object difference and obtain meta-bolites, can adopt inclined plane inoculating, liquid inoculation etc., current inoculation not yet can realize automated operation.
Bacterial classification often adopts the mechanism of amplifying step by step to carry out spawn activity, adaptive enhancing during the fermentation, finally ferments fast and efficiently, produces and accumulate a large amount of object product in thalline or in fermented liquid.Amplify step by step and namely carry out the even multistage seed culture of one-level, secondary by a small amount of inclined-plane of preservation or liquid spawn with certain inoculum size (5-10% as volume ratio), to improve spawn activity, to adapt to fermentation condition.Amplify step by step and be applied in microorganism cells, microalgae cell, the pilot scale culture of zooblast and the prior fermentation experiment in laboratory, particularly liquid fermenting process.Therefore guarantee liquid seeds culture transferring process safety, fast and convenient operation is one of prerequisite of carrying out smoothly of fermenting.
Usually triangular flask is adopted when carrying out one-level or secondary seed cultivation in laboratory, for guaranteeing the homogeneity inoculating seed in each bottle of fresh liquid substratum, the method of many employings " one-to-many ", i.e. by one bottle of seed liquor (or cultivating the seed liquor after merging with a collection of), by certain inoculum size, with transfer pipet or pipettor, liquid seeds is evenly accessed in many bottles of fresh liquid substratum, even if in last access liquid fermentation medium, in laboratory be also one bottle to the operator scheme of many bottles.
The aseptic technique of seeded process is carried out usually in laboratory in Bechtop, carries out in factorial praluction at sterilisable chamber, and both all need to utilize ultraviolet to article surface sterilization 20-30 minute before use.Mention when telling about the ultimate principle of aseptic technique in " industrial microbiology experimental technique " (Tianjin science tech publishing house), miscellaneous bacteria in air easily falls with dust at air-flow hour and causes microbiological contamination, therefore in seeded process, the time of opening culture vessel is as far as possible short, mouth will tilt and should in the sterile field of flame zone (i.e. spirit lamp flame kernel radius 5 centimetres), and operational requirement is skilled accurately fast.In the seed transfer process of factorial praluction, as far as possible the principle of aseptic technique reduces the chance of seed bottle opening, can pressurize with should adopt negative pressure or the sterile air of pressure as far as possible.Therefore for the operation of this one-to-many, for avoiding from the beginning microbiological contamination, the bottleneck of all triangular flasks should be tilted in operation, and the sterile field being placed in flame zone is interior, pulling out of bottle stopper of minimizing of trying one's best fills in number of times, ensures that skilled operation is accurately quick simultaneously.
The operation of this one-to-many, during by one man operation, need to grip seed liquor and fresh medium two bottles on the other hand, another hand is after the other bung off of flame, with liquid-transfering device, seed liquor sucking-off is reinjected in fresh culture, then the stopper lid crossing flame is returned two bottlenecks.Transfer second bottle time, same one hand holds same seed liquor and another bottle of fresh medium, repeats the same operation.Therefore, for seed liquor, whole seeded process needs repeatedly to pull out in aseptic operating platform to cover into bottle stopper, as for a bottled amount being 250 milliliters of triangular flasks of 100 milliliters, if accessing 10 with the inoculum size of 10% (volume ratio) bottledly has in 250 milliliters of next stage liquid mediums of 90 milliliters, so this bottle of seed liquor bottle needs repeatedly to pull out and covers into 10 bottle stoppers.Such operation not only have impact on the flowing of germ-free air flow in operator's console, easily causes the early stage microbiological contamination of liquid fermentation and culture because bringing miscellaneous bacteria into, and all needs to utilize flame to carry out sterilising treatment to two stoppers at every turn, extends the time of inoculation.For the personnel that inoculation experience is not enriched, in the other operation carrying out two bottles of flame, not only speed slowly and easily cause burning accident.Particularly often use in large-scale production for the large triangular flask of loading amount (triangular flask as 500 milliliters or 1 liter); ordinary person's one hand grips two simultaneously and has any problem, and can only stand upright in operator's console by triangular flask, pull out bottle stopper; after drawing seed liquor, then cover into bottle stopper.This operation, due to bottleneck directly on be exposed in air, one miscellaneous bacteria be the increase in air is directly mixed into the probability of seed liquor, in addition due to the limited space of Bechtop, or by the restriction of cover plate pull-out altitude, too increase the operation easier of pipettor in worktable (as transfer pipet).For the problems referred to above, the solution adopted is operated by two people more at present, a people holds seed bottle, all the time seed bottleneck is aimed at flame, another people holds fresh medium bottle and is responsible for sucking-off and the injection of seed liquor, so coordinates and the bottleneck of seed liquor can be kept to tilt and reduce microbiological contamination chance, also accelerate inoculation speed simultaneously, but consume manpower, and reduce inoculation efficiency.In large-scale production, transferred species or the inoculation that spreads cultivation carry out at sterilisable chamber; along with the increase of operator's quantity; also greatly add the chance of bringing miscellaneous bacteria into, therefore need a kind of inoculation of design utensil badly, it is very necessary for solving the single inoculation operation completing large loading amount triangular flask in large-scale production.
Summary of the invention
The object of the present invention is to provide liquid cell to cultivate special inoculation frame, operator can complete the inoculation operation of single liquid seeds by it.
Overall technology design of the present invention is:
Liquid cell cultivates special inoculation frame, comprises base plate, the back up pad that end and base plate are rotatably assorted; Support plate upper surface rear portion is provided with baffle plate, and support plate upper surface front portion is provided with bottleneck fixed mechanism, is provided with angular adjustment apparatus between back up pad and base plate.
Concrete technical conceive of the present invention also has:
The Main Function of bottleneck fixed mechanism is stable with what keep it in seeded process for the bottleneck of fixed triangle bottle, and comparatively preferred technical scheme is, described bottleneck fixed mechanism select the one in fixing strip, movable clamp or its combine.For ensureing fixation and not easily causing damage to frangible Glass Containers, can it is evident that fixing strip adopts flexible-belt, comprising but be not limited to and adopt with the flexible-belt of snap-fastener, velcro or bandage, material can be selected and comprise the soft Fiber Materials such as cotton, chemical fibre; Movable clamp can select arc clamp that is symmetrical and that driven by elastic element, or at least the multiple technologies scheme such as the arc top clamp opened of side realizes, and does not all depart from essence of the present invention.The working face of described arc clamp should also be equipped with flexo cushion to slow down the impact of colliding and causing with the bottleneck external shape fits of triangular flask.
More optimize and easy structure design is that described bottleneck fixed mechanism selects fixing strip, and the fixed orifices that fixing strip one end and support plate upper surface are offered is fixed, and fixed orifices symmetry is opened in support plate upper surface both sides.It can be made like this to be applicable to the triangular flask bottleneck of differing capacities specification, and comparatively easy structure design is that fixing strip is provided with velcro.
Be convenient to for making fixing strip adjust the highly different triangular flask of rear adaptation, more preferred structure design is that described fixed orifices selects bar hole.
The effect of angle-adjusting mechanism is convenient to make the present invention be applicable to different triangular flasks, the position residing for its bottleneck is made to be positioned at flame aseptic area with the needs of satisfied inoculation, this mechanism can adopt multiple conventional physical construction to realize, comprising but be not limited to adopt hydraulic stem, parallel-crank mechanism, leading screw feed mechanism, locating detent mechanism, worm-and-wheel gear, pinion and rack, cam mechanism, one in line motor or its combine.
More preferred design is that angular adjustment apparatus selects locating detent mechanism, and locating detent mechanism comprises locating dowel and the regulating rod that correspondence is arranged at back up pad and base plate side, regulating rod is intervally distributed with the adjusting hole with locating dowel adaptation.The adjusting hole of distribution on it and locating dowel snap fit can be made to realize the angular setting of back up pad and base plate by rotating regulating rod.
For ease of setting of the present invention, preferred technical scheme is, described base plate lower surface is provided with footing.
For ease of adjusting the height of base plate and back up pad further, more preferred structure design is that described footing selects adjustment bolt.
Described base plate is coordinated by axis of rotation with back up pad.
Adaptive for meeting the present invention's triangular flask different from capacity specifications, preferred structure design is, the adjusting hole that described baffle plate lower surface is offered by adjustment screw and back up pad surface is connected.
For verifying technique effect of the present invention, applicant carried out following experiment:
1, as inoculated the bottled fresh culture of 20 bottles of use, 1 liter of triangle, existing operating method needs two skilled operation personnel to complete for about 20 minutes; By the present invention, within single 20 minutes, complete.
2, as inoculated the bottled fresh culture of 20 bottles of use, 100 milliliters of triangles, for avoiding microbiological contamination in existing operating method, in factory, two about 15 minutes of skilled operation personnel (once holding two bottles of fresh cultures)-30 minutes (once holding one bottle of fresh culture) are usually needed to complete; By the present invention, singlely can to complete 15 minutes (once holding two bottles of fresh cultures).
3, as inoculated the bottled fresh culture of 20 bottles of use, 100 milliliters of triangles, if one man operation in existing operating method, the microbiological contamination rate of 1-2% can be caused in testing laboratory, seed liquor bottle stopper needs repeatedly to pull out, fill in 20 times, act frequently, have impact on air flowing, run counter to the principle that inoculation operation is light, fast, accurate, whole process need about 30-40 minute completes; And seed liquor bottle is because of repeatedly close to spirit lamp flame, bottleneck temperature is high, is easy to cause scald, also have impact on inoculation operation. by the present invention, seed liquor bottle stopper only needs to pull out, fill in 1 time, and within single about 15 minutes, (once holding two bottles of fresh cultures) can complete.
Substantive distinguishing features acquired by the present invention and significant technical progress are:
1, the ingenious principle according to gusseted of the present invention has carried out structure design, and the supporting structure of its base plate and back up pad composition is reliable and stable, and its one-piece construction is simple, easy to clean.
2, gripping of the triangular flask of different size is applicable to by adjusting angle adjustment mechanism, bottleneck fixed mechanism easily to make it, achieve seed bottle bottleneck in seeded process tilt all the time and be in the aseptic area of flame, effectively ensure that the stability of inoculation operating process intermediate cam bottle, and meet the sterility requirements in inoculation operation.
3, need not folding bottle stopper repeatedly in seeded process, operator singlely can complete seed liquor fast and draw and switch over operation, while reducing the microbiological contamination possibility in manipulation strength and seeded process, effectively improve inoculation speed.
4, after adopting the present invention, the triangular flask bottleneck of splendid attire seed liquor is obliquely installed, in single inoculation operation, for the operator that can be hold by one hand two low capacity triangular flasks, the triangular flask of two bottles of splendid attire fresh mediums can be held at every turn, such per minute can inoculate two bottles of fresh mediums, compare existing inoculation method, inoculation speed is doubled.Inoculation operation for Large Copacity triangular flask can singlely under of the present invention assisting complete smoothly, and cost of labor reduces 1 times.In addition, because only needing one man operation and without the need to bottle stopper being plugged repeatedly the air flowing caused in seeded process, greatly reducing microbiological contamination possibility, improve the success ratio of aseptic technique and ensure that follow-up spreading cultivation and the carrying out smoothly of industrial fermentation processes.
Accompanying drawing explanation
Fig. 1 is one-piece construction schematic diagram of the present invention.
Fig. 2 is the A direction view of Fig. 1.
Reference numeral in accompanying drawing is as follows:
1, baffle plate; 2, screw is adjusted; 3, regulating rod; 4, back up pad; 5, base plate; 6, fixing strip; 7, footing; 8, rotating shaft; 9, fixed orifices; 10, adjusting hole.
Embodiment
Drawings illustrate embodiments of the invention; below in conjunction with accompanying drawing, embodiments of the invention are described further; but should not be construed as limitation of the invention; the content that protection scope of the present invention is recorded with claim is as the criterion; any according to the equivalent technical elements replacement done by specification sheets, all do not depart from protection scope of the present invention.
The overall technology structure of the present embodiment, as diagram, comprises base plate 5, the back up pad 4 that end and base plate 5 are rotatably assorted; Back up pad 4 upper surface rear portion is provided with baffle plate 1, and back up pad 4 upper surface front portion is provided with bottleneck fixed mechanism, is provided with angular adjustment apparatus between back up pad 4 and base plate 5.
Described bottleneck fixed mechanism selects fixing strip 6, and the fixed orifices 9 that fixing strip 6 one end and back up pad 4 upper surface are offered is fixed, and fixed orifices 9 symmetry is opened in back up pad 4 upper surface both sides, and fixing strip is provided with velcro.
Described fixed orifices 9 selects bar hole.
Angular adjustment apparatus selects locating detent mechanism, and locating detent mechanism comprises the locating dowel that correspondence is arranged at back up pad 4 side, is arranged at the regulating rod 3 of base plate 5 side, regulating rod 3 is intervally distributed with the adjusting hole with locating dowel adaptation.The adjusting hole of distribution on it and locating dowel snap fit can be made to realize the angular setting of back up pad and base plate by rotating regulating rod.
Described base plate lower surface is provided with footing, and described footing selects adjustment bolt.
Described base plate 5 is rotatably assorted by rotating shaft 8 with back up pad 4.
Described baffle plate 1 lower surface is connected with the adjusting hole 10 that back up pad 4 surface is offered by adjustment screw 2.
Claims (10)
1. liquid cell cultivates special inoculation frame, comprises base plate (5), the back up pad (4) that end and base plate (5) are rotatably assorted; It is characterized in that back up pad (4) upper surface rear portion is provided with baffle plate (1), back up pad (4) upper surface front portion is provided with bottleneck fixed mechanism, is provided with angular adjustment apparatus between back up pad (4) and base plate (5).
2. liquid cell according to claim 1 cultivates special inoculation frame, it is characterized in that described bottleneck fixed mechanism select one in fixing strip, movable clamp or its combine.
3. the liquid cell according to any one of claim 1 cultivates special inoculation frame, it is characterized in that described angular adjustment apparatus select one in hydraulic stem, parallel-crank mechanism, leading screw feed mechanism, locating detent mechanism, worm-and-wheel gear, pinion and rack, cam mechanism, line motor or its combine.
4. the liquid cell according to any one of claim 1 or 2 cultivates special inoculation frame, it is characterized in that described base plate (5) lower surface is provided with footing (7).
5. liquid cell according to claim 4 cultivates special inoculation frame, it is characterized in that described footing (7) selects adjustment screw.
6. the liquid cell according to any one of claim 1 or 2 cultivates special inoculation frame, it is characterized in that described bottleneck fixed mechanism selects fixing strip (6), the fixed orifices (9) that fixing strip (6) one end and back up pad (4) upper surface are offered is fixed, and fixed orifices symmetry is opened in back up pad (4) upper surface both sides.
7. liquid cell according to claim 6 cultivates special inoculation frame, it is characterized in that described fixed orifices (9) selects bar hole.
8. the liquid cell according to any one of claim 1 or 3 cultivates special inoculation frame, it is characterized in that described angular adjustment apparatus selects locating detent mechanism, locating detent mechanism comprises locating dowel and the regulating rod (3) that correspondence is arranged at back up pad (4) and base plate (5) side, regulating rod (3) is intervally distributed with the adjusting hole with locating dowel adaptation.
9. liquid cell according to claim 1 cultivates special inoculation frame, it is characterized in that described base plate (5) and back up pad (4) are rotatably assorted by rotating shaft (8).
10. liquid cell according to claim 1 cultivates special inoculation frame, it is characterized in that described baffle plate (1) lower surface is connected with the adjusting hole (10) that back up pad (4) surface is offered by adjustment screw (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510180645.2A CN104762198A (en) | 2015-04-16 | 2015-04-16 | Special inoculation frame for liquid cell culture |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510180645.2A CN104762198A (en) | 2015-04-16 | 2015-04-16 | Special inoculation frame for liquid cell culture |
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| CN104762198A true CN104762198A (en) | 2015-07-08 |
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| CN201510180645.2A Pending CN104762198A (en) | 2015-04-16 | 2015-04-16 | Special inoculation frame for liquid cell culture |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107513496A (en) * | 2016-06-17 | 2017-12-26 | 上海市农药研究所有限公司 | Unicellular alga auto culturing system and its application |
| CN108546633A (en) * | 2018-04-17 | 2018-09-18 | 贵州省中国科学院天然产物化学重点实验室 | A kind of auxiliary device that culture dish uses |
| CN109355166A (en) * | 2018-11-26 | 2019-02-19 | 舟山医院 | A kind of simplicity Tissue Culture Flask operating device |
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| CN104437711A (en) * | 2014-12-23 | 2015-03-25 | 顺昊细胞生物技术（天津）股份有限公司 | Centrifugal pipe frame |
| CN204529876U (en) * | 2015-04-16 | 2015-08-05 | 润科生物工程（福建）有限公司 | Liquid cell cultivates special inoculation frame |
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2015
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| CN101389410A (en) * | 2006-02-24 | 2009-03-18 | 生物辐射实验室股份有限公司 | Sample plate support of adjustable angular orientation |
| CN201055779Y (en) * | 2007-03-30 | 2008-05-07 | 陈曦 | Adjustable reagent bottle frame |
| CN102665913A (en) * | 2009-07-31 | 2012-09-12 | 西蒙·斯塔福德 | Means for improved liquid handling in a microplate |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107513496A (en) * | 2016-06-17 | 2017-12-26 | 上海市农药研究所有限公司 | Unicellular alga auto culturing system and its application |
| CN108546633A (en) * | 2018-04-17 | 2018-09-18 | 贵州省中国科学院天然产物化学重点实验室 | A kind of auxiliary device that culture dish uses |
| CN109355166A (en) * | 2018-11-26 | 2019-02-19 | 舟山医院 | A kind of simplicity Tissue Culture Flask operating device |
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Application publication date: 20150708 |
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