CN206666501U - A kind of multi-functional nitrogen-fixing bacteria culture apparatus - Google Patents
A kind of multi-functional nitrogen-fixing bacteria culture apparatus Download PDFInfo
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- CN206666501U CN206666501U CN201720248929.5U CN201720248929U CN206666501U CN 206666501 U CN206666501 U CN 206666501U CN 201720248929 U CN201720248929 U CN 201720248929U CN 206666501 U CN206666501 U CN 206666501U
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- Prior art keywords
- fixing bacteria
- nitrogen
- culture apparatus
- culture vessel
- heater
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Abstract
The utility model discloses a kind of multi-functional nitrogen-fixing bacteria culture apparatus, mainly including casing, culture vessel, constent temperature heater, passage, outage, heater control handle, liquid filling hole, ventilation filter core, graduated scale, watch window；Can be in the research of microorganism fixed nitrogen, nitrogen-fixing bacteria are cultivated, ice is separated with gas to filter the vapor in gas, when needing certain temperature culture, it is necessary to be heated using constant incubator, growth is conveniently counted and is observed to strain number, error is small, being not susceptible to other impurity factors influences, efficiency high, and structure is accurate.
Description
Technical field
The utility model belongs to nitrogen-fixing bacteria culture device design field, is specifically to be related to a kind of multi-functional fixed nitrogen
Bacterium culture apparatus.
Background technology
Nitrogen-fixing bacteria are cultivated under experimental conditions, only carbon source and a small amount of inorganic salts need to be added in culture medium, are not required to add nitrogen source,
Nitrogen-fixing bacteria directly can be used as nitrogen nutrition by the use of the nitrogen in air；Rhizobium are such as cultivated, then need to add nitrogen nutrition, because root nodule
The symbiotic nitrogen-fixing bacterias such as bacterium, only with corresponding plant symbiosis when, could utilize molecular nitrogen carry out nitrogen fixation.
The biological energy source for being only capable of fixed nitrogen is survived in nitrogen-free agar, and diazotroph includes azotobacter and symbiosis is consolidated
The class the latter of nitrogen bacterium two must first be unable to fixed nitrogen, could be grown after the symbiont that can not be survived in nitrogen-free agar.Fixed nitrogen
Microorganism can fix the nitrogen in air, and in nitrogen-free culture, 18-40 DEG C of temperature, bacterial strain can grow and have azotase
The temperature of activity, its most suitable growth and fixed nitrogen is 26-37 DEG C；Under conditions of meta-acid (pH value 5.0) and meta-alkali (pH value 8.0),
Bacterial strain can keep stronger growth potential and higher nitrogenase activity, and can by adjust own metabolism adaptation environment acid,
Alkali changes, and makes nutrient solution convergence neutral；In nutrient solution NaCl concentration in 0.5-2.5g.L-1, (NH4) 2SO4 concentration in 0.05-
During 0.50g.L-1, bacterial strain can keep vigorous growth and have higher nitrogenase activity.
In the research of microorganism fixed nitrogen, often need to cultivate nitrogen-fixing bacteria, conventional blake bottle is all common thin mouth
Bottle, it is impossible to separate with air, the vapor in gas can not be filtered during aerating, when needing certain temperature culture, needed
To be heated using constant incubator, when needing to count strain number, flow is complicated, and error is big, easily by other
Impurity factor influence.
Utility model content
The technical problem that the utility model solves, which there is provided one kind, can provide heated at constant temperature, carry out liquid base and gas
The multi-functional nitrogen-fixing bacteria culture apparatus of multi-nutrition thing.
The technical solution of the utility model is as follows：A kind of multi-functional nitrogen-fixing bacteria culture apparatus, described device mainly include
Casing, culture vessel, constent temperature heater, passage, outage, heater control handle, liquid filling hole, ventilation filter core, graduated scale,
Watch window；Casing is screwed in the top of constent temperature heater, and culture vessel is placed on the heating surface of constent temperature heater,
By pipeline connected drainage hole, liquid filling hole, watch window is set in the top of culture vessel respectively for culture vessel, watch window
Surrounding is pasted with graduated scale, and ventilation filter core is provided between culture vessel and passage.
Further, described culture vessel is that have glass container made of silica, a diameter of 10-20cm, height
For 15-25cm, thickness 3-5mm, handled by high temperature resistant, maximum heating temperature is 300 DEG C.
Further, the heating temperature range of described constent temperature heater is 30-299 DEG C, is passed through on constent temperature heater
Temperature display carrys out displays temperature, and described constent temperature heater relies on thermistor heating unit heats, described heater control
Knob processed connects thermistor heating unit.
Further, multiple layers of filters is provided with described ventilation filter core, described filter layer includes gum sprayed cotton filter layer
And active carbon layer.
Further, described watch window is section of circular transparent resin sheet, its size and culture vessel
Area is identical.
Further, the minimum scale of described graduated scale is mm.
Further, described passage, outage, be fitted with valve on liquid filling hole.
A kind of multi-functional nitrogen-fixing bacteria culture apparatus operating method is as follows：
The first step：The microbial azotobacteria nutrient solution prepared is injected into culture vessel by liquid filling hole, valve is closed, leads to
Heater control handle is crossed to open constent temperature heater and set corresponding temperature.
Second step：According to conventional fixed nitrogen culture process, gas is added by passage, closes valve.
3rd step：Cultivated according to temperature, time as defined in fixed nitrogen culture process, pass through observation window in incubation
The morphological feature of mouth observation bacterium colony, passes through outage discharge opeing after the completion of culture.
Compared with prior art, device of the present utility model can be trained in the research of microorganism fixed nitrogen to nitrogen-fixing bacteria
Support, separate ice with gas and the vapor in gas is filtered, when needing certain temperature culture, it is necessary to using incubated
Case heats, and is conveniently counted and observed growth to strain number, error is small, it is not easy to influenceed by other impurity factors,
Efficiency high, structure are accurate.
Brief description of the drawings
Fig. 1 is a kind of structural representation of multi-functional nitrogen-fixing bacteria culture apparatus described in the utility model；
Wherein, 1- casings, 2- culture vessels, 3- constent temperature heaters, 4- passages, 5- outages, the control rotation of 6- heaters
Button, 7- liquid filling holes, 8- ventilations filter core, 9- graduated scales, 10- watch windows.
Embodiment
It is new below in conjunction with this practicality to make the purpose, technical scheme and advantage of the utility model embodiment clearer
The accompanying drawing of type, the technical scheme in the embodiment of the utility model is clearly and completely described, it is clear that described implementation
Example is the utility model part of the embodiment, rather than whole embodiments.Based on the embodiment in the utility model, this area
The every other embodiment that those of ordinary skill is obtained under the premise of creative work is not made, belongs to the utility model
The scope of protection.
For ease of the understanding to the utility model embodiment, below in conjunction with the accompanying drawings and the specific embodiments exemplified by do further
Explanation, embodiment do not form the restriction to the utility model embodiment.
Embodiment 1：As shown in figure 1, a kind of multi-functional nitrogen-fixing bacteria culture apparatus, described device mainly includes casing 1, training
Support container 2, constent temperature heater 3, passage 4, outage 5, heater control handle 6, liquid filling hole 7, ventilation filter core 8, graduated scale
9th, watch window 10；Casing 1 is screwed in the top of constent temperature heater 3, and training is placed on the heating surface of constent temperature heater 3
Container 2 is supported, by pipeline connected drainage hole 5, liquid filling hole 7, observation window is set in the top of culture vessel 2 respectively for culture vessel 2
Mouth 10, the surrounding of watch window 10 is pasted with graduated scale 9, and ventilation filter core 8 is provided between culture vessel 2 and passage 4.
Wherein, described culture vessel 2 is that have glass container made of silica, a diameter of 10cm, is highly 15cm,
Thickness is 3mm, is handled by high temperature resistant, and maximum heating temperature is 300 DEG C.On constent temperature heater 3 by temperature display come
Displays temperature, described constent temperature heater 3 rely on thermistor heating unit heats, and described heater control handle 6 connects
Thermistor heating unit.Multiple layers of filters is provided with described ventilation filter core 8, described filter layer includes gum sprayed cotton filter layer
And active carbon layer.Described watch window 10 is circular transparent resin sheet, its size and the sectional area of culture vessel 2
It is identical.The minimum scale of described graduated scale 9 is mm.Described passage 4, outage 5, valve is fitted with liquid filling hole 7.
A kind of multi-functional nitrogen-fixing bacteria culture apparatus operating method is as follows：
The first step：The microbial azotobacteria nutrient solution prepared is injected into culture vessel 2 by liquid filling hole 7, closes valve,
Constent temperature heater 3 is opened by heater control handle 6 and corresponding temperature is set.
Second step：According to conventional fixed nitrogen culture process, gas is added by passage 4, closes valve.
3rd step：Cultivated according to temperature, time as defined in fixed nitrogen culture process, pass through observation window in incubation
Mouth 10 observes the morphological feature of bacterium colonies, passes through the discharge opeing of outage 5 after the completion of culture.
Embodiment 2：As shown in figure 1, a kind of multi-functional nitrogen-fixing bacteria culture apparatus, described device mainly includes casing 1, training
Support container 2, constent temperature heater 3, passage 4, outage 5, heater control handle 6, liquid filling hole 7, ventilation filter core 8, graduated scale
9th, watch window 10；Casing 1 is screwed in the top of constent temperature heater 3, and training is placed on the heating surface of constent temperature heater 3
Container 2 is supported, by pipeline connected drainage hole 5, liquid filling hole 7, observation window is set in the top of culture vessel 2 respectively for culture vessel 2
Mouth 10, the surrounding of watch window 10 is pasted with graduated scale 9, and ventilation filter core 8 is provided between culture vessel 2 and passage 4.
Wherein, described culture vessel 2 is that have glass container made of silica, a diameter of 15cm, is highly 20cm,
Thickness is 4mm, is handled by high temperature resistant, and maximum heating temperature is 300 DEG C.On constent temperature heater 3 by temperature display come
Displays temperature, described constent temperature heater 3 rely on thermistor heating unit heats, and described heater control handle 6 connects
Thermistor heating unit.Multiple layers of filters is provided with described ventilation filter core 8, described filter layer includes gum sprayed cotton filter layer
And active carbon layer.Described watch window 10 is circular transparent resin sheet, its size and the sectional area of culture vessel 2
It is identical.The minimum scale of described graduated scale 9 is mm.Described passage 4, outage 5, valve is fitted with liquid filling hole 7.
A kind of multi-functional nitrogen-fixing bacteria culture apparatus operating method is as follows：
The first step：The microbial azotobacteria nutrient solution prepared is injected into culture vessel 2 by liquid filling hole 7, closes valve,
Constent temperature heater 3 is opened by heater control handle 6 and corresponding temperature is set.
Second step：According to conventional fixed nitrogen culture process, gas is added by passage 4, closes valve.
3rd step：Cultivated according to temperature, time as defined in fixed nitrogen culture process, pass through observation window in incubation
Mouth 10 observes the morphological feature of bacterium colonies, passes through the discharge opeing of outage 5 after the completion of culture.
Embodiment 3：As shown in figure 1, a kind of multi-functional nitrogen-fixing bacteria culture apparatus, described device mainly includes casing 1, training
Support container 2, constent temperature heater 3, passage 4, outage 5, heater control handle 6, liquid filling hole 7, ventilation filter core 8, graduated scale
9th, watch window 10；Casing 1 is screwed in the top of constent temperature heater 3, and training is placed on the heating surface of constent temperature heater 3
Container 2 is supported, by pipeline connected drainage hole 5, liquid filling hole 7, observation window is set in the top of culture vessel 2 respectively for culture vessel 2
Mouth 10, the surrounding of watch window 10 is pasted with graduated scale 9, and ventilation filter core 8 is provided between culture vessel 2 and passage 4.
Wherein, described culture vessel 2 is that have glass container made of silica, a diameter of 20cm, is highly 25cm,
Thickness is 5mm, is handled by high temperature resistant, and maximum heating temperature is 300 DEG C.On constent temperature heater 3 by temperature display come
Displays temperature, described constent temperature heater 3 rely on thermistor heating unit heats, and described heater control handle 6 connects
Thermistor heating unit.Multiple layers of filters is provided with described ventilation filter core 8, described filter layer includes gum sprayed cotton filter layer
And active carbon layer.Described watch window 10 is circular transparent resin sheet, its size and the sectional area of culture vessel 2
It is identical.The minimum scale of described graduated scale 9 is mm.Described passage 4, outage 5, valve is fitted with liquid filling hole 7.
A kind of multi-functional nitrogen-fixing bacteria culture apparatus operating method is as follows：
The first step：The microbial azotobacteria nutrient solution prepared is injected into culture vessel 2 by liquid filling hole 7, closes valve,
Constent temperature heater 3 is opened by heater control handle 6 and corresponding temperature is set.
Second step：According to conventional fixed nitrogen culture process, gas is added by passage 4, closes valve.
3rd step：Cultivated according to temperature, time as defined in fixed nitrogen culture process, pass through observation window in incubation
Mouth 10 observes the morphological feature of bacterium colonies, passes through the discharge opeing of outage 5 after the completion of culture.
It should be noted last that above example is only unrestricted to illustrate the technical solution of the utility model, to the greatest extent
The utility model is described in detail with reference to preferred embodiment for pipe, it will be understood by those within the art that, can be with
The technical solution of the utility model is modified or equivalent substitution, without departing from technical solutions of the utility model spirit and
Scope.
Claims (7)
1. a kind of multi-functional nitrogen-fixing bacteria culture apparatus, it is characterised in that described device mainly includes casing (1), culture vessel
(2), constent temperature heater (3), passage (4), outage (5), heater control handle (6), liquid filling hole (7), ventilation filter core
(8), graduated scale (9), watch window (10)；Casing (1) is screwed in the top of constent temperature heater (3), heated at constant temperature
Culture vessel (2) is placed on the heating surface of device (3), and culture vessel (2) passes through pipeline connected drainage hole (5), liquid filling hole respectively
(7) watch window (10), is set in the top of culture vessel (2), the surrounding of watch window (10) is pasted with graduated scale (9), trains
Support and ventilation filter core (8) is provided between container (2) and passage (4).
A kind of 2. multi-functional nitrogen-fixing bacteria culture apparatus as claimed in claim 1, it is characterised in that described culture vessel (2)
It is to have glass container made of silica.
A kind of 3. multi-functional nitrogen-fixing bacteria culture apparatus as claimed in claim 1, it is characterised in that described constent temperature heater
(3) heating temperature range is 30-299 DEG C, described by temperature display come displays temperature on constent temperature heater (3)
Constent temperature heater (3) relies on thermistor heating unit heats, the connection thermistor heating of described heater control handle (6)
Unit.
A kind of 4. multi-functional nitrogen-fixing bacteria culture apparatus as claimed in claim 1, it is characterised in that described ventilation filter core (8)
Interior to be provided with multiple layers of filters, described filter layer includes gum sprayed cotton filter layer and active carbon layer.
A kind of 5. multi-functional nitrogen-fixing bacteria culture apparatus as claimed in claim 1, it is characterised in that described watch window (10)
It is identical with the sectional area of culture vessel (2) for the transparent resin sheet of circle, its size.
6. a kind of multi-functional nitrogen-fixing bacteria culture apparatus as claimed in claim 1, it is characterised in that described graduated scale (9)
Minimum scale is mm.
7. a kind of multi-functional nitrogen-fixing bacteria culture apparatus as claimed in claim 6, it is characterised in that described passage (4), row
Fluid apertures (5), liquid filling hole are fitted with valve on (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720248929.5U CN206666501U (en) | 2017-03-15 | 2017-03-15 | A kind of multi-functional nitrogen-fixing bacteria culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720248929.5U CN206666501U (en) | 2017-03-15 | 2017-03-15 | A kind of multi-functional nitrogen-fixing bacteria culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206666501U true CN206666501U (en) | 2017-11-24 |
Family
ID=60377203
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720248929.5U Expired - Fee Related CN206666501U (en) | 2017-03-15 | 2017-03-15 | A kind of multi-functional nitrogen-fixing bacteria culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206666501U (en) |
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2017
- 2017-03-15 CN CN201720248929.5U patent/CN206666501U/en not_active Expired - Fee Related
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171124 Termination date: 20200315 |
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| CF01 | Termination of patent right due to non-payment of annual fee |