CN114107010A - Device and method for automatically and synchronously coating culture dishes in multiple areas - Google Patents

Device and method for automatically and synchronously coating culture dishes in multiple areas Download PDF

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Publication number
CN114107010A
CN114107010A CN202111406573.0A CN202111406573A CN114107010A CN 114107010 A CN114107010 A CN 114107010A CN 202111406573 A CN202111406573 A CN 202111406573A CN 114107010 A CN114107010 A CN 114107010A
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coating
culture dish
microorganism
coating device
telescopic mechanism
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CN202111406573.0A
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CN114107010B (en
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李秀萍
陆兵
郭云霞
张敬慧
兰小艳
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Yibin Vocational and Technical College
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Yibin Vocational and Technical College
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

Abstract

The invention discloses a device for automatically and synchronously coating a culture dish in multiple areas, which comprises a main body frame, wherein the main body frame comprises a bottom plate and a top plate, the top plate is provided with a plurality of positioning round holes, the positioning round holes are used for placing a dropping liquid pipe, the dropping liquid pipe penetrates through the positioning round holes, a microorganism bacterium suspension is dropped onto the culture dish through the dropping liquid pipe, a driving device is used for driving a coating device to uniformly coat the microorganism bacterium suspension, and the driving device controls the coating device to coat microorganisms, so that the risk of mutual pollution among the microorganisms is greatly reduced compared with manual coating; in addition, many dropping liquid pipes can be put simultaneously to a plurality of locating holes, and many dropping liquid pipes can be used for the synchronous dropping liquid of multiple microorganism, and coating unit also can carry out the coating of multiple microorganism and multizone simultaneously, can reduce the required time of coating culture dish in a large number.

Description

Device and method for automatically and synchronously coating culture dishes in multiple areas
Technical Field
The invention relates to the field of microorganism culture dish partition culture, in particular to a device and a method for automatically and synchronously coating a culture dish in multiple areas.
Background
In microbial culture, it is often necessary to perform a plating operation of the microorganisms in order to count, separate and purify the microorganisms. At present, the microorganism culture dish coating method mainly depends on manual operation, and only one microorganism suspension can be coated each time, so that the operation is time-consuming and labor-consuming. Meanwhile, only one kind of microorganism is usually cultured on one culture dish, which causes waste of resources. There are also a few reports in literature, which are that different microorganisms are cultured in multiple regions in a culture dish, but the microorganism coating method used by the microorganism culture dish method still depends on manual operation, and the microorganisms are coated on the culture dish one by one carefully, and the operation easily causes mutual pollution among different microorganisms, and is time-consuming and labor-consuming.
Disclosure of Invention
The invention aims to provide a device and a method for automatically and synchronously coating a culture dish in multiple areas, which solve the problems that microorganisms are coated on the culture dish in multiple areas, the coating is easy to pollute, and the coating is time-consuming and labor-consuming.
The invention is realized by the following technical scheme:
the device for automatically and synchronously coating the culture dish in multiple areas comprises a main body frame, wherein the main body frame comprises a bottom plate with a placing part, the placing part is used for fixedly placing the culture dish, the main body frame also comprises a top plate with a plurality of positioning round holes, the positioning round holes are used for placing a dropping liquid pipe, and the dropping liquid pipe is used for dropping liquid to the culture dish; the bottom plate supports the top plate through the fixed supporting piece; the main body frame is also provided with a coating device and a driving device; the driving device is used for driving the coating device to uniformly coat the microorganism suspension.
The device realizes the automatic multi-zone synchronous culture dish coating: the dropping liquid pipe penetrates through the positioning round hole, the microbial suspension is dropped onto the culture dish through the dropping liquid pipe, the driving device is used for driving the coating device to uniformly coat the microbial suspension, and the driving device controls the coating device to coat the microorganisms, so that the risk of mutual pollution among the microorganisms is greatly reduced compared with manual coating; in addition, many dropping liquid pipes can be put simultaneously to a plurality of locating holes, and many dropping liquid pipes can be used for the synchronous dropping liquid of multiple microorganism, and coating unit also can carry out the coating of multiple microorganism and multizone simultaneously, can reduce the required time of coating culture dish in a large number.
Further, the placing part is a culture dish groove arranged on the center of the bottom plate, the culture dish groove is matched with the culture dish, and the depth of the culture dish groove is not larger than the side height of the culture dish. The position of culture dish is for fixed culture dish to culture dish recess setting, and culture dish recess degree of depth is not more than culture dish side height and is more convenient for when taking out the culture dish.
Further, fixed support piece sets up to two backup pads, is greater than the culture dish recess diameter apart from between two backup pads, and is parallel to each other and all perpendicular to take over a business and bottom plate connection between two backup pads. The fixed support piece is arranged as a support plate, so that when liquid dropping is carried out, more liquid is blocked at two sides, and side pollution is prevented.
Further, the positioning round holes are evenly distributed around the center circumference of the top disc. The purpose is to evenly drip microbial suspension on the culture dish, and especially when the culture dish is used for various microbial species, the drip pipes which are evenly distributed can be inserted into the evenly distributed positioning round holes, thereby completing even coating.
Furthermore, a telescopic mechanism is arranged at the center of the top disc and connected with a coating device, the coating device comprises a connecting rod, one end of the connecting rod is connected with the telescopic mechanism, the other end of the connecting rod is provided with a hollow cylinder, and a plurality of coating paddles are arranged on the circumference of the hollow cylinder; the coating paddle is provided with a liquid dropping hole, the position of the liquid dropping hole is opposite to that of the limiting and positioning round hole on the top plate, and a coating groove with an opening facing the culture dish is arranged on the coating paddle. The telescopic mechanism is used for extending and retracting the coating device, and the hollow cylinder is used for isolating the blank area from the center of the culture dish and preventing microorganisms from being polluted in the middle area of the culture dish.
Further, the driving device is used for driving the coating device to rotate, and driving the coating paddle to rotate on the culture dish according to a set value, so that the microbial suspension is uniformly coated. The driving device is set as a motor, and the forward and reverse rotation of the motor drives the forward and reverse rotation of the coating device, so that the coating paddle is driven to rotate on the culture dish according to a set value and is used for uniformly coating the microbial suspension.
Furthermore, a control system is further arranged on the top disc, a pressure sensing device is arranged between the telescopic mechanism and the coating device, the pressure sensing device is used for sensing the pressure at the end part of the coating device, and the control system is used for receiving signals of the pressure sensing device and controlling the telescopic mechanism to stretch. The pressure sensing device is used for receiving pressure signals, the pressure signals are transmitted to the control system, the control system processes the pressure signals, and the pressure signals are converted and then used for controlling the stretching and retracting of the stretching mechanism.
A coating method of an automatic multi-zone synchronous culture dish coating device comprises the following steps:
s1, determining the types of microorganisms and the number of the coating areas, and attaching a drip tube and a corresponding coating device; determining the type and the coating quantity of microorganisms, and then arranging the corresponding dripping pipes and the number of the coating paddles on the coating device, wherein the dripping pipes and the coating paddles correspond to the number of the coating areas one by one.
S2, taking down the culture dish cover, and placing the culture dish in the culture dish groove on the bottom plate;
s3, connecting one end of a high temperature resistant hose connected with a peristaltic pump to the bacteria solution bottle, connecting the other end of the high temperature resistant hose to the dropping liquid tube, starting the peristaltic pump, and extracting the microorganism bacteria suspension until the microorganism bacteria suspension reaches the experiment set value, and stopping the peristaltic pump; the peristaltic pump is used for pumping the microorganism bacterium suspension in the bacterium liquid bottle.
S4: after the peristaltic pump stops, the coating device is extended to the culture dish by the telescopic mechanism, and the coating device is controlled by the motor to perform coating movement through a set coating area and the type of the microorganism;
s5: after the coating movement is finished, the telescopic mechanism contracts the coating device to enable the coating device to recover the initial position, and the telescopic mechanism stops working to finish the whole coating work.
Further, coating unit, dropping liquid pipe, high temperature resistant hose and fungus liquid bottle all need carry out high pressure steam sterilization, stoving and ultraviolet sterilization before the use, and above-mentioned step is all accomplished in superclean bench.
Furthermore, a telescopic mechanism is arranged on one side, close to the bottom plate, of the center of the top plate, a pressure sensing device is arranged between the telescopic mechanism and the coating device, and when the bottom of the coating device touches the culture dish, the pressure sensing device senses pressure, so that the telescopic mechanism stops extending.
Compared with the prior art, the invention has the following advantages and beneficial effects:
a device for automatically and synchronously coating culture dishes in multiple areas comprises a main body frame, wherein the main body frame comprises a bottom plate and a top plate, the top plate is provided with a plurality of positioning round holes, the positioning round holes are used for placing a dropping liquid pipe, the dropping liquid pipe penetrates through the positioning round holes, microbial suspension is dripped onto the culture dishes through the dropping liquid pipe, a driving device is used for driving a coating device to uniformly coat the microbial suspension, and the driving device controls the coating device to coat microorganisms, so that the risk of mutual pollution among the microorganisms is greatly reduced compared with manual coating; in addition, many dropping liquid pipes can be put simultaneously to a plurality of locating holes, and many dropping liquid pipes can be used for the synchronous dropping liquid of multiple microorganism, and coating unit also can carry out the coating of multiple microorganism and multizone simultaneously, can reduce the required time of coating culture dish in a large number.
Drawings
In order to more clearly illustrate the technical solutions of the exemplary embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and that for those skilled in the art, other related drawings can be obtained from these drawings without inventive effort. In the drawings:
FIG. 1 is a schematic three-dimensional structure diagram of an entire device according to an embodiment of the present invention;
FIG. 2 is a schematic structural view of a detailed view of a coating device of the present device according to an embodiment of the present invention;
FIG. 3 is a schematic structural view of a detailed view of a coating paddle A of the apparatus according to an embodiment of the present invention;
FIG. 4 is a schematic diagram illustrating the distribution of four areas of a same microorganism in a culture dish coated simultaneously with four areas according to an embodiment of the present invention;
FIG. 5 is a schematic diagram showing the distribution of four regions coated with the same microorganism in a culture dish in synchronization with each other according to the embodiment of the present invention;
FIG. 6 is a schematic diagram showing the distribution of three zones of three microorganisms coated simultaneously in a culture dish according to an embodiment of the present invention;
FIG. 7 is a schematic diagram of the distribution of the areas, from left to right, of a single area, two areas, three areas and four areas, of the same microorganism in different numbers of areas coated synchronously on a single culture dish according to an embodiment of the present invention;
fig. 8 is a schematic front view of the whole device according to the embodiment of the present invention.
Reference numbers and corresponding part names in the drawings:
1-bottom plate, 2-culture dish groove, 3-support plate, 4-top plate, 5-positioning round hole, 6-control system, 7-timing switch, 8-contactless switch, 9-motor, 10-telescopic mechanism, 11-pressure sensing device, 12-coating device, 1201-coating paddle, 1202-hollow cylinder, 1203-connecting rod, 1204-coating groove, 1205-dropping hole, 13-dropping tube, 14-high temperature resistant flexible tube, 15-peristaltic pump and 16-bacteria liquid bottle.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to examples and accompanying drawings, and the exemplary embodiments and descriptions thereof are only used for explaining the present invention and are not meant to limit the present invention.
Example 1
As shown in fig. 1-3, an apparatus for automatically coating culture dishes in multiple areas synchronously comprises a main frame, wherein the main frame comprises a bottom plate 1 with a placing part for fixedly placing the culture dishes, and a top plate 4 with a plurality of positioning round holes 5, the positioning round holes 5 are used for placing a dropping liquid pipe 13, and the dropping liquid pipe 13 is used for dropping liquid to the culture dishes; the bottom plate 1 supports the top plate 4 through a fixed support; the main body frame is also provided with a coating device 12 and a driving device; the driving device is used for driving the coating device 12 to uniformly coat the microorganism suspension.
The device realizes the automatic multi-zone synchronous culture dish coating: the liquid dropping pipe 13 penetrates through the positioning round hole 5, the microbial suspension is dropped onto the culture dish through the liquid dropping pipe 13, the driving device is used for driving the coating device 12 to uniformly coat the microbial suspension, and the driving device controls the coating device 12 to coat the microorganisms, so that the risk of mutual pollution among the microorganisms is greatly reduced compared with manual coating; in addition, many dropping liquid pipes 13 can be put simultaneously to a plurality of locating holes, and many dropping liquid pipes 13 can be used for the synchronous dropping liquid of multiple microorganism, and coating unit 12 also can carry out the coating of multiple microorganism and multizone simultaneously, can reduce the required time of coating culture dish in a large number.
The bottom plate 1 is used for accommodating a culture dish and fixing the two support plates 3; the culture dish groove 2 on the bottom plate 1 is positioned in the center of the bottom plate 1, the shape of the culture dish groove 2 is consistent with that of a culture dish, the culture dish groove 2 is cylindrical, and the central axis of the culture dish groove 2 is positioned on the same vertical line with the central axis of the coating device 12, the pressure induction device 11, the telescopic mechanism 10, the motor 9 and the top plate 4; the supporting piece preferably comprises two supporting plates 3 which are respectively erected on two sides of a culture dish groove 2 on the bottom plate 1 and have equal distance with the central point of the culture dish groove 2, the two supporting plates 3 are parallel to each other, and the two supporting plates 3 are respectively fixedly connected with the top plate 4 and the bottom plate 1 from top to bottom and are used for fixing the top plate 4 and the bottom plate 1; the top plate is a circular plate, a plurality of positioning round holes 5 which are circular and are uniformly distributed at equal intervals are arranged in the top plate at a certain distance from the two supporting plates 3, the center point of the top plate 4 is used as the center of a circle, the diameter of each positioning round hole 5 is smaller than the top of the dripping pipe 13 and is used for containing the dripping pipe 13, the distance between every two positioning round holes 5 on the same top plate 4 diameter does not exceed the diameter of the culture dish groove 2, and the dripping pipe 13 can be ensured to drip the microorganism suspension liquid onto the culture dish after being installed;
the portion of placing is for setting up the culture dish recess 2 at bottom plate 1 center, culture dish recess 2 and culture dish adaptation, and the culture dish recess 2 degree of depth is not more than the culture dish side height. The culture dish groove 2 is arranged for fixing the position of the culture dish, and the depth of the culture dish groove 2 is not more than the height of the side edge of the culture dish, so that the culture dish can be taken out conveniently.
Fixed support piece sets up to two backup pads 3, is greater than culture dish recess 2 diameters between two backup pads 3 at a distance from, and is parallel to each other and all is connected with bottom plate 1 in the top dish 4 of equal perpendicular to between two backup pads 3. The fixed support piece is arranged as the supporting plate 3, so that when liquid dropping is carried out, more liquid is blocked at two sides, and side pollution is prevented.
The positioning round holes 5 are evenly distributed around the center circumference of the top disk 4. The purpose is to evenly drip microbial suspension on the culture dish, and especially when the culture dish is used for various microbial species, the uniformly distributed dripping pipes 13 can be inserted and distributed on the uniformly distributed positioning round holes 5, thereby completing even coating.
The center of the top plate 4 is provided with a telescopic mechanism, the telescopic mechanism is connected with a coating device 12, the coating device 12 comprises a connecting rod 1203, one end of the connecting rod 1203 is connected with the telescopic mechanism 10, the other end of the connecting rod 1203 is provided with a hollow cylinder 1202, and a plurality of coating paddles 1201 are arranged on the circumference of the hollow cylinder 1202; the coating paddle 1201 is provided with a dropping hole 1205, the dropping hole 1205 is opposite to the limiting and positioning round hole 5 on the top plate 4, and the coating paddle 1201 is provided with a coating groove 1204 with an opening facing the culture dish. The telescoping mechanism is used to extend and retract the applicator 12 and the hollow cylinder 1202 is provided to isolate the central region of the culture dish from the blank area and prevent contamination of microorganisms in the middle region of the culture dish.
The coating device 12 is composed of a top threaded connecting rod 1203, a hollow cylinder 1202 with a downward opening at the bottom, and a coating paddle 1201 fixedly connected. The top thread of the connecting rod 1203 is used for connecting the coating device 12 with the pressure sensing device 11 in a threaded manner; the hollow cylinder 1202 with the downward opening at the bottom is fixedly connected with the connecting rod 1203 without a thread, the coating paddles 1201 are uniformly distributed on the circumference of the hollow cylinder 1202, and the hollow cylinder 1202 with the downward opening is used for forming a circular blank area on the center of the culture medium so as to prevent microorganisms in different areas from being polluted mutually; evenly distributed coating paddles 1201 are used for microbial coating of different areas on the medium. For example: the number of the coating paddles 1201 of the coating device 12 can be n (n is more than or equal to 2 and is an integer), and synchronous coating of a plurality of areas on one culture dish is realized by replacing the coating devices 12 with different numbers of the coating paddles 1201. The coating paddle 1201 of the coating device 12 rotates by X degrees (X is more than or equal to 0 and less than or equal to 365), which is determined by the number of coated areas, and when only 1 coated area exists, the X degree is 360 degrees; when there are n (n is not less than 2, and n is an integer) coating regions, X DEG is 360 DEG/n-5 deg. To prevent contamination, the angle of the blank area may be increased according to the kind of microorganism.
The telescopic mechanism 10 is connected under the motor 9 and used for the pressure sensing device 11 and the coating device 12 to move up and down in the vertical direction. When coating is needed, the telescopic mechanism 10 extends to enable the coating device 12 to move downwards to contact with a culture medium on a culture dish, and the pressure sensing device 11 senses a certain pressure to enable the telescopic mechanism 10 to stop extending; after the coating is completed, the telescopic mechanism 10 is contracted to raise the pressure-sensitive device 11 and the coating device 12 until the starting position is restored.
The driving device is used for driving the coating device 12 to rotate, driving the coating paddle 1201 to rotate on the culture dish according to a set value, and coating the microbial suspension uniformly. The driving device is set as a motor 9, and the forward and reverse rotation of the motor 9 drives the forward and reverse rotation of the coating device 12, so as to drive the coating paddle 1201 to rotate on the culture dish according to a set value for uniformly coating the microorganism suspension. The motor 9 is embedded and fixed at the central point of the top disc 4, and passes through the top disc 4 right below to be connected with the telescopic mechanism 10. The device is used for driving the telescopic mechanism 10, the pressure sensing device 11 and the coating device 12 to rotate forwards and backwards at a certain angle so as to realize multi-zone synchronous coating of microorganisms on the culture dish; a timing switch 7 and a non-contact switch 8 are arranged on the motor 9 and are positioned above the motor 9, and the timing switch 7 is used for controlling the rotation time of the motor 9 so as to stop the coating operation after the coating operation is performed for a certain time; the contactless switch 8 is positioned above the motor 9 and is used for controlling the forward and reverse rotation of the motor 9.
The top disc 4 is further provided with a control system 6, a pressure sensing device 11 is arranged between the telescopic mechanism 10 and the coating device 12, the pressure sensing device 11 is used for sensing the pressure at the end part of the coating device 12, and the control system 6 is used for receiving signals of the pressure sensing device 11 and controlling the telescopic mechanism 10 to stretch. The pressure sensing device 11 is used for receiving a pressure signal, the pressure signal is transmitted to the control system 6, the control system 6 processes the pressure signal, and the pressure signal is converted and then used for controlling the stretching of the stretching mechanism 10.
The pressure sensing device 11 is connected to the position right below the telescoping mechanism 10 and is used for sensing the pressure of the culture medium on the culture dish contacted by the coating device 12 in the downward movement process, so as to control the telescoping mechanism 10 to stop extending. An internal thread round hole is arranged right below the pressure sensing device 11 and is used for threaded connection of the top of the connecting rod 1203 of the coating device 12; the control system 6 is fixed on the top disc 4, is in the same vertical plane with one support plate 3, and is used for controlling the actions and the operation of the contactless switch 8, the motor 9, the telescopic mechanism 10 and the pressure induction device 11.
A coating method of an automatic multi-zone synchronous culture dish coating device comprises the following steps:
s1, determining the types of microorganisms and the number of the coating areas, and attaching the drip tube 13 and the corresponding coating device 12; the types and the coating quantity of microorganisms are determined, and the quantity of the coating paddles 1201 on the corresponding dropping liquid pipes 13 and the corresponding coating device 12 is arranged to correspond to the quantity of the coating areas one by one.
Wherein the dropping liquid pipe 13 is a hollow stainless steel pipe, the bottom end is a needle point shape, the middle pipe diameter is smaller than the diameter of the positioning round hole 5, the top end has a small section of pipe diameter larger than the diameter of the positioning round hole 5, so that the bottom end and the middle part of the dropping liquid pipe 13 can pass through the positioning round hole 5 and be hung on the top disc 4. The dropping tube 13 is used for dropping the microorganism suspension which is originally contained in the microorganism liquid bottle 16 and is transmitted by the high temperature resistant hose 14 onto the culture medium. The microorganism suspension in the microorganism liquid bottle 16 is delivered to the dropping liquid tubes 13 of different positioning round holes 5 through different delivery routes and is dropped on different areas of the culture medium. For example: when the number of the batch of coating dropping liquid distributing pipes 13 is 1, 1 positioning round hole 5 can be selected optionally; when the number of the drip tubes 13 for coating in the batch is more than or equal to 2, the drip tubes 13 need to be inserted into the corresponding positioning round holes 5 in pairs at equal intervals.
Wherein the bacteria liquid bottle 16 is a high temperature resistant bottle with a positioning round hole 5 sealing cover on the top and is used for containing the microorganism bacteria suspension. The high temperature resistant hose 14 can extend into the bacteria bottle 16 through the top positioning round hole 5 to convey the microorganism bacterial suspension. When different microbial suspensions need to be coated, corresponding bacteria liquid bottles 16 can be additionally arranged for containing.
S2, taking down the culture dish cover, and placing the culture dish in the culture dish groove on the bottom plate;
s3, connecting one end of a high temperature resistant hose 14 connected with a peristaltic pump 15 to the bacteria liquid bottle 16, and connecting the other end of the high temperature resistant hose to the dropping liquid pipe 13, starting the peristaltic pump 15, and extracting the microorganism bacteria suspension until the microorganism bacteria suspension reaches the experiment set value, and stopping the peristaltic pump 15; the peristaltic pump 15 is used for pumping bacteria liquid in the bacteria liquid bottle 16.
Wherein the high temperature resistant hose 14 is a hollow hose, one end of the hose is inserted into the bacteria liquid bottle 16, the middle of the hose passes through the peristaltic pump 15, and the other end of the hose is sleeved on the top of the dropping liquid tube 13. The high temperature resistant hose 14 is used for transporting the microorganism suspension in the microorganism solution bottle 16. When different microorganisms need to be conveyed, corresponding high-temperature-resistant hoses 14 and the dropping liquid pipes 13 at different positions can be added and are connected in a one-to-one correspondence manner, so that different microorganisms can be dropped on different areas of the culture medium.
The peristaltic pump 15 is a multi-channel peristaltic pump 15 and has different flow delivery functions. Is used for pumping the microorganism bacterium suspension in the bacterium liquid bottle 16 into the high temperature resistant hose 14, and leading the microorganism bacterium suspension to finally drop on the culture medium through the high temperature resistant hose 14 and the dropping liquid pipe 13. The flow rate of the peristaltic pump 15 for delivering the microorganism suspension is determined according to the concentration of the microorganism suspension in the microorganism solution bottle 16 or the size of the upper area of the culture medium, and when the flow rate reaches a set value, the peristaltic pump 15 stops working. When different microbial suspensions need to be conveyed, the high-temperature-resistant hose 14 can be added, and different high-temperature-resistant hoses 14 pass through different channels of the peristaltic pump 15, so that the conveying of different microbial suspensions is realized. For example: when the number of the drip tubes 13 coated in the batch is n (n is more than or equal to 1, and n is an integer), the peristaltic pump 15 stops working when the microbial suspension dripped from each drip tube 13 is 200/n microliter.
S4: after the peristaltic pump 15 stops, the coating device 12 is extended to the culture dish by the telescopic mechanism, and the coating device 12 is controlled by the motor 9 to perform coating movement through a set coating area and microorganism types;
s5: after the coating movement is finished, the coating device 12 is contracted by the telescopic mechanism to be restored to the initial position, and the telescopic mechanism stops working to finish the whole coating work.
The coating device 12, the dropping liquid pipe 13, the high temperature resistant hose 14 and the bacteria liquid bottle 16 are all required to be sterilized by high pressure steam, dried and sterilized by ultraviolet light before use, and the steps are all finished in a super clean bench.
The center of the top plate 4 is provided with a telescopic mechanism near one side of the bottom plate 1, a pressure sensing device 11 is arranged between the telescopic mechanism 10 and the coating device 12, and when the bottom of the coating device 12 touches the culture dish, the pressure sensing device 11 senses pressure, so that the telescopic mechanism 10 stops extending.
The principle of the whole device is as follows: the apparatus comprises an apparatus main body frame, a control system 6, and a drive system. The device main body frame comprises a bottom plate 1 and a culture dish groove 2 arranged at the center of the bottom plate, two support plates 3 are arranged on the bottom plate 1 and are uniformly distributed on two sides of the culture dish groove 2, and the two support plates 3 are fixedly connected with a top plate 4 and the bottom plate 1 from top to bottom respectively. The top disc 4 is provided with circular positioning round holes 5 which are uniformly distributed at equal intervals, and the positioning round holes 5 are used for hanging and placing the dropping liquid tubes 13; one end of a high temperature resistant hose 14 extends into the bacteria liquid bottle 16 through a connecting hole at the top of the bacteria liquid bottle 16, the other end of the high temperature resistant hose 14 penetrates through a peristaltic pump 15 and is connected with the top of the dropping liquid pipe 13, and bacteria liquid enters the dropping liquid pipe 13 through the high temperature resistant hose 14 and then drops on a culture dish; the control system 6 device is arranged on the top disc 4 at the same vertical line position with the support plate 3, so that the weight of the placing system can find a bearing point by placing the control system 6 device; the motor 9 is embedded in the center of the top disc 4, and the upper part of the motor 9 is provided with a timing switch 7 and a non-contact switch 8. The timing switch 7 is used for controlling the rotation time of the motor 9 so as to stop the coating operation after the coating operation is performed for a certain time; the contactless switch 8 is used for controlling the forward and reverse rotation of the motor 9, and the forward and reverse rotation of the motor 9 can drive the forward and reverse rotation of the coating device 12, so that the coating operation is completed. The lower portion of the motor 9 is connected with a telescopic mechanism 10, a pressure sensing device 11 is connected under the telescopic mechanism 10, a screw hole is formed in the lower portion of the pressure sensing device 11 and used for being in threaded connection with a coating device 12, the telescopic mechanism 10 is used for controlling the stretching of the coating device 12, a control signal is transmitted to the control system 6 through the pressure of the end portion of the coating device 12 sensed by the pressure sensing device 11, and then the stretching of the telescopic mechanism 10 is controlled.
The principle of the coating device 12 is: the coating paddle 1201 is a groove with a downward opening, a cylindrical drip hole 1205 is arranged at the top of the groove, a left side plate of the groove is a left coating plate, a front side plate is a front baffle plate, a right side plate is a right coating plate to form a coating frame, and the tail part of the coating frame is connected with the hollow cylinder 1202 of the coating device 12. A small semi-cylindrical protrusion is arranged at the uppermost part of the threaded hole at the lower part of the pressure sensing device 11, and a semi-cylindrical notch corresponding to the protrusion is arranged at the top part of the connecting rod 1203 of the coating device 12 and can be matched with each other. When the top of the coating unit 12 is screwed into the threaded hole at the lower part of the pressure sensing unit 11, the notch at the top of the coating unit 12 hits the semi-cylindrical protrusion, so that the dropping hole 1205 of the coating paddle 1201 is at the same vertical position with the dropping pipe 13 hung right above it. The microorganism suspension dropped from the dropping tube 13 passes through the dropping hole 1205 of the coating paddle 1201 and drops on the culture medium of the culture dish, and at this time, the microorganism suspension on the culture medium is in the space surrounded by the coating frame of the coating paddle 1201 and the hollow cylinder 1202, so that the microorganism suspensions in different areas are prevented from being contaminated with each other when coating. When the coating device 12 rotates clockwise by X degrees, the microorganism bacterial suspension is coated by the left coating plate of the coating frame; when the coating device 12 is rotated X ° counter-clockwise, the microbial suspension is now coated by the right coating plate of the coating frame. Repeating the above steps for a plurality of times, and after a period of time, starting the timing switch 7 and stopping the motor 9. The coating paddle 1201 of the coating device 12 rotates by X degrees (X is more than or equal to 0 and less than or equal to 365), which is determined by the number of coated areas, and when only 1 coated area exists, the X degree is 360 degrees; when there are n (n is not less than 2, and n is an integer) coating regions, X DEG is 360 DEG/n-5 deg.
Example 2
As shown in FIG. 4, a method for automatically coating multiple zones of a culture dish in a synchronous manner, which is exemplified by four-zone synchronous coating of the same kind of microorganisms in one culture dish, comprises the following steps:
1. the coating device 12, the dropping liquid pipe 13, the high temperature resistant hose 14 and the bacteria liquid bottle 16 are wrapped by newspaper and sterilized by high pressure steam at 121 ℃ for 20 min. And (4) completely sterilizing, and drying the raw materials in an oven at 60 ℃ for later use.
2. And placing the sterilized dropping liquid tube 13, the high-temperature-resistant hose 14, the bacteria liquid bottle 16 and other parts of the device in a super-clean workbench, and performing ultraviolet sterilization for 30 min.
3. And opening the superclean workbench, and performing the following operations in the superclean workbench.
4. The microbial suspension is added into the bacteria liquid bottle 16 through a small hole on the bottle cap of the bacteria liquid bottle 16. The coating device 12 is rotated by the top thread into the threaded hole below the pressure sensing device 11. The tips of the four dropping tubes 13 penetrate through four positioning round holes 5 on the top plate 4 at equal intervals and are hung on the top plate 4. One end of each of four high temperature resistant hoses 14 is extended into the bottom of a bacteria liquid bottle 16, the other end of each of the four high temperature resistant hoses 14 is sleeved on the top of each of four dropping liquid pipes 13, and the middle parts of the four high temperature resistant hoses 14 are wound into peristaltic pumps 15.
5. The petri dish with the solid culture medium is placed in the petri dish groove 2 after the top cover of the petri dish is opened.
6. The peristaltic pump 15 is started, and the microorganism suspension in the microorganism liquid bottle 16 is dripped to four equidistant areas of the culture medium along the four high temperature resistant hoses 14 through the four dripping pipes 13. When the microbial suspension dropped from each dropping tube 13 is 50 microliters, the peristaltic pump 15 stops working.
7. The control system 6 is started to control the extension mechanism 10 to extend, and when the bottom of the coating device 12 touches the culture medium, the pressure sensing device 11 senses the pressure, so that the extension mechanism 10 stops extending. Then the motor 9 is started, the coating device 12 is driven to rotate clockwise by 85 degrees to leave a partial blank 5 degrees, the area of the blank area can be adjusted according to different types of requirements of microorganisms, and the microorganisms in different areas are prevented from being polluted mutually; the contactless switch 8 is started, the motor 9 drives the coating device 12 to rotate 85 degrees anticlockwise, the contactless switch 8 is started again, the coating device 12 is driven to rotate 85 degrees clockwise again, and the operation is repeated for a plurality of times, so that the four coating paddles 1201 of the coating device 12 uniformly coat the microbial suspension dropped on the culture medium on four areas of the culture medium. After a certain time, the timing switch 7 is started and the motor 9 stops rotating.
8. The telescopic mechanism 10 is shortened to restore the pressure-sensitive device 11 and the coating device 12 to the initial positions, and the telescopic mechanism 10 stops operating.
9. And taking the coated culture dish out of the culture dish groove 2 to finish the coating operation.
It should be explained that:
(1) if a plurality of culture dishes need to be coated with the microbial bacterial suspension, the culture dishes are replaced by new ones, and the operation is repeated.
(2) If the number of the microorganism areas to be coated needs to be changed, the coating device 12 with the corresponding number of the coating paddles 1201, the high temperature resistant hose 14 and the dropping liquid pipe 13 need to be replaced, and the operations are repeated.
(3) If another microorganism suspension needs to be coated on another culture dish, the coating device 12, the dropping liquid pipe 13, the high temperature resistant hose 14 and the bacteria liquid bottle 16 need to be replaced by new ones, and the operations are repeated.
Example 3
As illustrated in fig. 5, based on embodiment 2, the present embodiment provides: four concentration gradients of the same microorganism are coated synchronously in one culture dish.
1. A sterilized dropping liquid pipe 13, a sterilized high-temperature resistant hose 14 and a sterilized liquid bottle 16 form a set of microorganism bacterium suspension conveying system, four sets of microorganism bacterium suspension conveying systems are taken, a coating device 12 with four coating paddles 1201 is configured, and four concentration gradient synchronous coating of the same kind of microorganism in a culture dish is completed according to the following operations.
2. Four bacteria liquid bottles 16 are respectively filled in a super clean workbench with a dilution gradient of 10-3、10-4、10-5、10-6The microbial suspension of (3). Four dropping tubes 13 are inserted into the positioning round holes 5 two by two at equal intervals and then connected with the device.
3. The peristaltic pump 15 is started, and the microbial suspension in the four microbial solution bottles 16 respectively drops to four different areas of the culture medium along the four high temperature resistant flexible tubes 14 and through the dropping liquid tubes 13. When the microbial suspension dripped from each dripping tube 13 is 200 microlitres, the peristaltic pump 15 stops working.
7. The control system 6 is started to control the extension mechanism 10 to extend, and when the bottom of the coating device 12 touches the culture medium, the pressure sensing device 11 senses the pressure, so that the extension mechanism 10 stops extending. Then the motor 9 is started, firstly the coating device 12 is driven to rotate 85 degrees clockwise, the non-contact switch 8 is started, the motor 9 drives the coating device 12 to rotate 85 degrees counterclockwise, the non-contact switch 8 is started again, the coating device 12 is driven to rotate 85 degrees clockwise again, and the operation is repeated for a plurality of times, so that the coating paddle 1201 of the coating device 12 drops on the culture medium to be 10 degrees-3、10-4、10-5、10-6The diluted microbial suspension of (4) is uniformly spread on four different areas of the medium, respectively. After a certain time, the timing switch 7 is started and the motor 9 stops rotating.
8. The telescopic mechanism 10 is shortened, the pressure sensing device 11 and the coating device 12 are restored to the initial positions, and the telescopic mechanism 10 stops operating.
9. And taking the coated culture dish out of the culture dish groove 2 to finish the synchronous coating operation of four concentration gradients of the same type of microorganisms in one culture dish.
Example 4
As illustrated in fig. 6, based on embodiment 2, the present embodiment provides: three areas of three microorganisms in one culture dish are coated synchronously.
1. A sterilized dropping liquid pipe 13, a sterilized high-temperature resistant hose 14 and a sterilized liquid bottle 16 are a set of microbial suspension conveying system, three sets of microbial suspension conveying systems are taken, a coating device 12 with three coating paddles 1201 is configured, and three-area synchronous coating of three types of microorganisms in a culture dish is completed according to the following operation.
2. Three bacteria liquid bottles 16 are respectively filled with microorganism suspension of microorganism a, microorganism b and microorganism c in a clean bench. Three drip tubes 13 are inserted into the positioning circular holes 5 equidistantly two by two, and then the device is connected according to figure 1.
3. The peristaltic pump 15 is started, and the microbial suspension in the three microbial solution bottles 16 respectively drops to three different areas of the culture medium along the three high temperature resistant flexible tubes 14 and through the dropping liquid tubes 13. When the microbial suspension dripped from each dripping tube 13 is 67 microlitres, the peristaltic pump 15 stops working.
7. The control system 6 is started to control the extension mechanism 10 to extend, and when the bottom of the coating device 12 touches the culture medium, the pressure sensing device 11 senses the pressure, so that the extension mechanism 10 stops extending. And then the motor 9 is started, the coating device 12 is firstly driven to rotate in the clockwise direction by 115 degrees, the non-contact switch 8 is started, the motor 9 drives the coating device 12 to rotate in the anticlockwise direction by 115 degrees, the non-contact switch 8 is started again, the coating device 12 is driven to rotate in the clockwise direction by 115 degrees again, and the steps are repeated for a plurality of times, so that the coating paddle 1201 of the coating device 12 respectively and uniformly coats the microbial strain suspensions of the microorganisms a, the microorganisms b and the microorganisms c which are dripped on the culture medium on three different areas of the culture medium. After a certain time, the timing switch 7 is started and the motor 9 stops rotating.
8. The telescopic mechanism 10 is shortened, the pressure sensing device 11 and the coating device 12 are restored to the initial positions, and the telescopic mechanism 10 stops operating.
9. The coated culture dish is taken out from the culture dish groove 2, and three areas of three microorganisms in one culture dish are coated synchronously.
Example 5:
as illustrated in fig. 7, based on embodiment 2, the present embodiment provides: simultaneous coating of different numbers of different areas of the same microorganism.
1. A sterilized dropping liquid pipe 13, a sterilized high-temperature resistant hose 14 and a sterilized liquid bottle 16 are a set of microbial liquid conveying system, one set, two sets, three sets and four sets of microbial liquid conveying system are respectively adopted, coating devices 12 with two, three and four coating paddles 1201 are respectively configured, and coating of different areas of microorganisms is completed in batches according to the following operations.
2. In the superclean bench, the bacteria liquid bottles 16 are all filled with the microorganism suspension, the dropping liquid tubes 13 are inserted into the positioning round holes 5 (when the number of the batch of coating and dispensing dropping liquid tubes 13 is 1, 1 positioning round hole 5 can be selected arbitrarily, and when the number of the batch of coating and dispensing dropping liquid tubes 13 is more than or equal to 2, the dropping liquid tubes 13 are inserted into the corresponding positioning round holes 5 at equal intervals in pairs), and then the device is connected according to the figure 1.
3. The peristaltic pump 15 is started, and the microbial suspension in the microbial solution bottle 16 is dripped onto the culture medium along the high temperature resistant hose 14 through the dripping tube 13. When the number of the liquid dropping pipes 13 for coating in the batch is one, two, three or four, respectively, the microbial suspension dropped from each liquid dropping pipe 13 is 200 microliters, 100 microliters, 67 microliters or 50 microliters, respectively, and the peristaltic pump 15 stops working.
7. The control system 6 is started to control the extension mechanism 10 to extend, and when the bottom of the coating device 12 touches the culture medium, the pressure sensing device 11 senses the pressure, so that the extension mechanism 10 stops extending. And then the motor 9 is started, the coating device 12 is firstly driven to rotate in the clockwise direction by X degrees, the non-contact switch 8 is started, the motor 9 drives the coating device 12 to rotate in the anticlockwise direction by X degrees, the non-contact switch 8 is started again, the coating device 12 is driven to rotate in the clockwise direction by X degrees again, and the steps are repeated for a plurality of times, so that the coating paddle 1201 of the coating device 12 uniformly coats the microbial strain suspension dropped on the culture medium. When the coating regions are 1, 2, 3, 4, respectively, X ° is 360 °, 175 °, 115 °, 85 °, respectively. After a certain time, the timing switch 7 is started and the motor 9 stops rotating.
8. The telescopic mechanism 10 is shortened, the pressure sensing device 11 and the coating device 12 are restored to the initial positions, and the telescopic mechanism 10 stops operating.
9. And taking the coated culture dish out of the culture dish groove 2 to finish the coating operation.
It should be explained that: in the figures of the various embodiments, the white part is a sterile blank medium, and the shaded part is a microorganism-coated area.
The above-mentioned embodiments are intended to illustrate the objects, technical solutions and advantages of the present invention in further detail, and it should be understood that the above-mentioned embodiments are merely exemplary embodiments of the present invention, and are not intended to limit the scope of the present invention, and any modifications, equivalent substitutions, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.

Claims (10)

1. The device for automatically and synchronously coating the culture dishes in multiple areas is characterized by comprising a main body frame, wherein the main body frame comprises a bottom plate (1) with a placing part, the placing part is used for fixedly placing the culture dishes, the main body frame further comprises a top plate (4) with a plurality of positioning round holes (5), the positioning round holes (5) are used for placing a dropping liquid pipe (13), and the dropping liquid pipe (13) is used for dropping liquid to the culture dishes; the bottom plate (1) supports the top plate (4) through a fixed support piece;
the main body frame is also provided with a coating device (12) and a driving device;
the driving device is used for driving the coating device (12) to uniformly coat the microorganism suspension.
2. The device for automatically and synchronously coating the culture dishes in multiple areas as claimed in claim 1, wherein the placing part is a culture dish groove (2) arranged on the center of the bottom plate (1), the culture dish groove (2) is matched with the culture dish, and the depth of the culture dish groove (2) is not more than the height of the side edge of the culture dish.
3. An apparatus for automatic multi-zone simultaneous coating of culture dishes according to claim 1, wherein the fixed support is provided as two support plates (3), the distance between the two support plates (3) is larger than the diameter of the culture dish groove (2), and the two support plates (3) are parallel to each other and are both perpendicular to the top plate (4) and the bottom plate (1) to be connected.
4. An apparatus for automated multi-zone simultaneous coating of culture dishes according to claim 1, wherein the positioning circular holes (5) are evenly distributed circumferentially around the center of the top plate (4).
5. The device for automatically and synchronously coating the culture dishes in multiple areas according to claim 1, wherein a telescopic mechanism (10) is arranged in the center of the top plate (4), the telescopic mechanism (10) is connected with the coating device (12), the coating device (12) comprises a connecting rod, one end of the connecting rod is connected with the telescopic mechanism (10), the other end of the connecting rod is provided with a hollow cylinder (1202), a plurality of coating paddles (1201) are arranged on the circumference of the hollow cylinder (1202), and the driving device is used for driving the coating paddles (1201) to rotate.
6. The device for automatically coating the culture dish in the multi-zone synchronous manner according to claim 5, wherein the coating paddle (1201) is provided with a dropping hole (1205), the dropping hole (1205) is opposite to a limiting and positioning round hole (5) on the top plate (4), and the coating paddle (1201) is provided with a coating groove (1204) with an opening facing the culture dish.
7. The device for automatically and synchronously coating the culture dishes in multiple areas according to claim 1, wherein a control system (6) is further arranged on the top plate (4), a pressure sensing device (11) is arranged between the telescoping mechanism (10) and the coating device (12), the pressure sensing device (11) is used for sensing the end pressure of the coating device (12), and the control system (6) is used for receiving a signal of the pressure sensing device (11) and controlling the telescoping mechanism (10) to telescope.
8. Method for the automatic coating of the devices for the simultaneous multi-zone coating of culture dishes according to any one of claims 1 to 7, characterized in that:
s1, determining the types of microorganisms and the number of the coating areas, and attaching a dropping liquid tube (13) and a corresponding coating device (12);
s2, taking down the culture dish cover, and placing the culture dish in the culture dish groove on the bottom plate;
s3, connecting one end of a high temperature resistant hose (14) connected with a peristaltic pump (15) to the bacteria liquid bottle (16), and connecting the other end of the high temperature resistant hose to the liquid dropping pipe (13), starting the peristaltic pump (15), and extracting the microorganism bacterial suspension until the peristaltic pump (15) stops when the microorganism bacterial suspension reaches the experiment set value;
s4: after the peristaltic pump (15) is stopped, the coating device (12) is extended to the culture dish by the telescopic mechanism (10), and the coating device (12) is controlled to carry out coating movement by the motor (9) through a set coating area and microorganism types;
s5: after the coating movement is finished, the telescopic mechanism (10) contracts the coating device (12) to enable the coating device to recover the initial position, the telescopic mechanism (10) stops working, and the whole coating work is finished.
9. The method for automatically and synchronously coating the culture dishes in multiple areas according to claim 8, wherein the coating device (12), the drip tube (13), the high temperature resistant hose (14) and the bacteria solution bottle (16) are sterilized by autoclaving, drying and UV sterilization before use, and the steps are performed in a clean bench.
10. The method for automatically and synchronously coating the culture dishes in multiple areas according to claim 8, wherein a telescopic mechanism (10) is arranged at one side of the center of the top plate (4) close to the bottom plate (1), a pressure sensing device (11) is arranged between the telescopic mechanism (10) and the coating device (12), and when the bottom of the coating device (12) touches the culture dishes, the pressure sensing device (11) senses the pressure, so that the telescopic mechanism (10) stops extending.
CN202111406573.0A 2021-11-24 2021-11-24 Device and method for automatically and synchronously coating culture dish in multiple areas Active CN114107010B (en)

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