IL34569A

IL34569A - Apparatus for the growth of living cells on a large scale

Info

Publication number: IL34569A
Application number: IL34569A
Authority: IL
Original assignee: Farmaceutici Italia
Priority date: 1969-05-23
Filing date: 1970-05-20
Publication date: 1973-10-25
Also published as: CA937519A; BR7019144D0; FR2051554A1; AT298373B; NO134062C; BE750837A; NL7006931A; DE2024763A1; JPS4824743B1; DK126046B; NO134062B; IL34569A0; GB1277544A; ZA703423B; AU1529870A; FR2051554B1; ES379927A1

Description

APPARATUS FOR THE GROWTH OF LIVING CELLS ON A LARGE SCALE The invention relates to the growth of living cells on a large Such growth is carried out in a monolayer of schizomycetes primary explants dipjoid cell continuous cell and biologicals for human or veterinary The production of vaccines for preventing virus diseases has passed through various the large susceptible domestic animal or small laboratory embryonated and finally cells monolayer or in Large amounts of living cells are necessary for the industrial scale production of vaccines These cells cannot be grown in suspension in fermenter vessels like the biosynthetic production of antibiotics or ergot The invention provides apparatus for growing living cells which comprises a number columns in which cells can end manifolds for securing the columns in and means for rotating the columns bodily about two Such apparatus can be designed to have considerable capacity in relation to its size because of the comparatively large interior surface area of the It is comparatively simple to being and other gases readily monitored and dosed with nutrient all under sterile The columns may be of plastic or other material for the of and may have g ana A be in a roots or thermostat for the th s a there the are in one by ducts at these tilted a that they of in a cylindrical d place an in application the TOliars central task to central the the the has been in a invention the 1 a elevation issantalt an elevation the drawings there are a number of columns 1 in cells can olds 6 for securing the columns 1 in and means for rotating the columns 1 bodily about vertical and horizontal Beyond the manifolds 6 are a number of cocks 3 leading to feed manifolds 2 and thence to feed tubes manifolds 2 and 6 are secured to a shaft and associated means for rotating the columns bodily about a horizontal The shaft is disengageable from the associated means and rotatably journalled respect to a general support frame 7 has legs The support frame 7 is rotata in a vertical plane about a shaft and by associated means All operations can be carried out by opening and closing cocks 3 at the appropriate Through the cocks 3 the columns 1 may be connected with different services tap water and deionised filling pipes discharging and drawing or gas required for growing the The columns may be connected in toto or individually not only with the different general services but also in a closed line and under sterile conditions with special devices for the distribution of duly measured and automatically recorded of liquids such as nutrient buffered cell suspensions trypsin or other virus or pure or mixed gases such as carbon A temporary connection is also possible with special breathing pipes for the carbon dioxide formed during the growth of the cells and with discharge liquids Such devices can provide for the measurement and automatic or recording of different Measuring and recording means may be connected with devices to correct and maintain the parameters within chosen Each column or any number of columns may be provided with means for measuring and recording the pH possibly with a device capable of maintaining the culture medium within a desired pH range either by varying the dioxide or by conveying buffered A microscope may be incorporated in the apparatus to permit observation of the cells in monolayer on the surfaces of the columns themselves at different magnifications all along the columns A preferred cycle of action washing of the apparatus with a detergent polution by vacuum connection and rinsing with deionised sterilization of the apparatus with seeding of the apparatus with cell suspension in a growth and growth of the sown cells on the surface of the columns rotating in horizontal The growth of the cells on the surface may be checked under the microscope as it is characterized by the formation of a thin opalescent layer on the surface of the When the growth is the cells are ready either for enzymatic scission for example with trypsin if the monolayer cells must be utilized for further or for the production of virus or other Once the cells or other biologicals have been the cycle can be begun again by washing the insufficientOCRQuality

Claims

HAVING NOW particularly described and ascertained the nature ofour said invention and in whart manner the same i§/£o be performed, we declare th t what we claim is: WHAT" IS Π.ATMPD IS:

1. Apparatus for growing living cells which comprises a number of columns in which cells can grow, end manifolds for securing the columns in position, and means for rotating the columns bodily about two axes.

2. Apparatus according to Claim 1 in which the axes are mutally perpendicular.

3. Apparatus according to Claim 1 or Claim 2 in which the columns and one of the axes are parallel.

4. „ Apparatus according to any of the preceding Claims which includes a thermostat surrounding the columns.

5. Apparatus according to any of the preceding Claims in which the columns are of glass.

6. Apparatus according to any of the preceding Claims which includes means for controlling and/or recording the volume of fluid introduced.

7. Apparatus according to any of the preceding Claims which includes means for controlling and/or recording the pH of liquid in the columns.

8. Apparatus according to any of the preceding Claims which includes means for controlling and/or recording the temperature of liquid in the columns.

9. Apparatus for growing living cells substantially as described with reference to the drawings. A t t o r n e y s f o r A p p l i c a n t