CN204762669U - Automatic experimental apparatus is cultivateed to accuse liquid plant tissue liquid - Google Patents
Automatic experimental apparatus is cultivateed to accuse liquid plant tissue liquid Download PDFInfo
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- CN204762669U CN204762669U CN201520502079.8U CN201520502079U CN204762669U CN 204762669 U CN204762669 U CN 204762669U CN 201520502079 U CN201520502079 U CN 201520502079U CN 204762669 U CN204762669 U CN 204762669U
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Abstract
The utility model relates to an automatic experimental apparatus is cultivateed to accuse liquid plant tissue liquid belongs to plant tissue culture technical field. The device includes the culture vessel, the culture vessel is by constituting with lower casing the last casing that closes sealing connection each other, the epitheca bodily form becomes the vegetation space and the top has the filtration transom, the casing is settled and is lain in the dish of growing seedlings on the culture solution down, include culture jar and surplus liquid collection box in addition, the culture jar through switching control valve and measuring pump and the casing feeds through down, surplus liquid collection box pass through positive displacement pump and on -off control valve and the bottom of casing feeds through down. Adopt the utility model discloses, can take the circumstances into consideration the required culture solution of supply as required to can realize down the convenient regulation and control of culture solution liquid level in the casing, and go up the casing and be convenient for open and close with constructing the stay knot of casing down, can conveniently take out plant and accurate return, thereby satisfy the various requirements in the experiment of plant tissue culture.
Description
Technical field
The utility model relates to a kind of Plant Tissue Breeding experimental provision, and especially a kind of automatic liquid control plant tissue liquid culture experimental provision, belongs to field of plant tissue culture technique.
Background technology
Plant Tissue Breeding is inoculated in the organ of plant, tissue in the medium container containing the composition such as nutriment and plant growth regulating substance of human configuration by sterile working, carries out cultured in vitro, make it grow into whole plant under manual control environment.Plant Tissue Breeding divides solid culture and the large mode of liquid culture two, wherein liquid culture mode adds culture fluid in tissue culture flasks, then the plant tissue of acquisition is put into culture fluid to cultivate, for solid culture, liquid culture have upgrade easily, without the need to changing container, facilitate filtration sterilization, cleaning amount is few, turn the advantages such as time second is few, be widely adopted in scientific research and actual production process.
Usually, the culture fluid for Plant Tissue Breeding does not need to change in an incubation, as long as supplement in good time.But in laboratory environment, in order to scientific research is explored, need the culture fluid changing different ratio, heterogeneity for different plant tissue under different cultivation stage, accelerate to make plant to grow up, shorten cultivation cycle, or need regularly to change plant organ soak time in a liquid, to prevent plant organ from producing vitrifying because being immersed in liquid for a long time, affect Plant Tissue Breeding quality.
In laboratory practices, there is following problems in this replacing culture fluid, adjustment culture fluid soak time etc.: 1) adopt and topple over mode and change liquid, need to take out plant tissue, easily cause plant tissue physical damnification; 2) open blake bottle and change liquid, difficulty or ease ensure aseptic condition, easily secondary pollution occur, and cause plant to fall ill dead; Topple over mode and change that the fluid exchange time is long, efficiency is low.Further, in process of experimental, often need regularly to take out institute in the training period and cultivate plant and carry out various cultural colony index determining, require plant taking-up, return operates light, efficient, plant injury can not be caused, affect late growing stage and experimental error.
But current plant liquid culture experiment container generally adopts the vial, polypropylene film bag etc. of the integral type of 200ml-250ml, be difficult between culture period accomplish quick, light taking-up and return culture experiment plant under guarantee does not damage the prerequisite of plant tissue.
Retrieval finds, the patent No. is that the Chinese patent of ZL201420873922.9 discloses one " new bio teaching practice plant cultivation device ", this device comprises casing and the chamber door hinged with casing side, casing inner bottom part arranges a base plate, on base plate, four corners are provided with column, and column is provided with dividing plate, and dividing plate ground is provided with several light source bodies, base plate both sides respectively arrange a refractive body, and base plate evenly arranges culture vessel.This patent mainly for the control of incubator inner light source, and does not relate to the technical problems such as culture fluid control above-mentioned in culture experiment, plant taking-up and return.In addition, the patent No. is that the Chinese patent of ZL201220200152.2 discloses " a kind of plant cultivation device ", comprise the first housing, the second housing, its inner space forming the accommodation medium supplying plant growth rear tied by first housing and the second housing card, but this patent does not relate to the technical problem that in experiment, culture fluid controls yet, and the plant tissue bogey limited space adopted, return are inaccurate, be unfavorable for experimental study and batch production.
Summary of the invention
The object of the invention is to: for above-mentioned prior art Problems existing, propose a kind ofly to realize culture fluid conveniently regulating and controlling, plant can the automatic liquid control plant tissue liquid culture experimental provision of convenient taking-up and return, thus meet experiment condition required in plant tissue's culture experiment.
In order to reach above object, the plant tissue liquid culture experimental provision basic technical scheme of automatic liquid control of the present invention is: comprise culture vessel, described culture vessel is by the involutory upper shell that is tightly connected and lower house are formed mutually, described upper shell forms plant growth space and top has filtration transom, and described lower house settles the seedling-cultivating tray be positioned on culture fluid; Comprise culture jar and remaining liquid collection box in addition, described culture jar is communicated with described lower house with measuring pump by switching control valve, and described remaining liquid collection box is communicated with the bottom of on-off control valve with described lower house by positive displacement pump.
In plant tissue culture course, measuring pump and switching control valve can be started as required, take the circumstances into consideration the culture fluid needed for supply, and by on-off control valve and tapping valve can be started, realize the conveniently regulating and controlling of culture fluid liquid level in lower house, and the butted structure of upper shell and lower house is convenient to open and close, can convenient taking-ups plant and accurate return, thus meet the various requirement in plant tissue's culture experiment.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the present invention is further illustrated.
Fig. 1 is the structural representation of a preferred embodiment of the invention.
Embodiment
Embodiment one
As shown in Figure 1, culture vessel 1 is made up of mutually involutory transparent upper shell 2 and lower house 3 the automatic liquid control plant tissue liquid culture experimental provision of the present embodiment.Upper shell 2 has relative flange respectively with lower house 3, has positioning spigot circle upward in the flange of lower house 3, and therefore both can mutually involutoryly be tightly connected, and open very convenient.Upper shell 2 forms plant growth space and top has two filtration transom 2-1, realizes the gas exchanges inside and outside container, and ensures the gnotobasis in container.
The circumferential interval of lower house 3 is equipped with the locating ring 3-1 of predetermined altitude, and for settling the seedling-cultivating tray 4 be positioned on culture fluid, seedling-cultivating tray 4 has the locating hole inserting fixing pot for growing seedlings 4-1, therefore can take out that plant is convenient, return is accurate.
In addition, multiple different types of culture jar is also furnished with---the present embodiment is two 5,5 ' and remaining liquid collection box 6.Culture jar 5,5 ' is communicated with lower house 3 with measuring pump 8 by the three-way magnetic valve 7 of switching control valve, can supplement the culture fluid of required kind in time as required.Remaining liquid collection box 6 is communicated with the bottom of on-off control valve 10 with lower house 3 by positive displacement pump 9, can regulate and control the liquid level of culture fluid in lower house 3.
In lower house 3, liquid level sensor 11 is also housed, its signal output part connects the controlled end of three-way magnetic valve 7, measuring pump 8, positive displacement pump 9, on-off control valve 10 respectively by the control circuit in controller 12, thus can realize the Automatic Control of the present embodiment device.
Be summed up, the experimental provision of the present embodiment has following features:
(1) in plant tissue culture course, by the change of liquid level sensor Real-Time Monitoring liquid level, controller opens three-way magnetic valve and measuring pump according to liquid level change, and the liquid level realizing culture fluid is controlled in real time, culture fluid needed for regular supply; Feed liquid method can adopt programme-control, by controlling liquid level, making plant organ regularly spill liquid level, to prevent plant organ from producing vitrifying in a liquid because of long-term submergence, avoiding affecting Plant Tissue Breeding quality.
(2) owing to being configured with two kinds of culture jars and remaining liquid collection box and corresponding valve member and the pump housing, two culture jars can be used alternatingly, in plant incubation, without the need to opening culture vessel, realize the replacing of variety classes culture fluid very easily, meet the conditional request changing different culture fluid in plant incubation in different phase.
(3) incubator upper and lower casing is tightly connected by demountable structure, upper shell can be opened easily between culture period, whole seedling-cultivating tray is taken out from container fast, cultural colony mensuration is carried out to wherein plant tissue, then overall return, then the upper and lower casing that is tightly connected; Taking-up and the return of culture experiment plant are light, quick, accurate, can not cause damage because being limited to vessel space.
(4) sabot cultivate time, by highly different locating rings select seedling-cultivating tray lay height, to adapt to the Plant Tissue Breeding needs of different growing way.
(5) upper shell end face is provided with air-permeative filter window, gas exchanges inside and outside container can be realized, and ensure the gnotobasis in container.
In addition to the implementation, the utility model can also have other embodiments.All employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop on the protection domain of the utility model requirement.
Claims (4)
1. an automatic liquid control plant tissue liquid culture experimental provision, comprise culture vessel, it is characterized in that: described culture vessel is by the involutory upper shell that is tightly connected and lower house are formed mutually, described upper shell forms plant growth space and top has filtration transom, and described lower house settles the seedling-cultivating tray be positioned on culture fluid; Comprise culture jar and remaining liquid collection box in addition, described culture jar is communicated with described lower house with measuring pump by switching control valve, and described remaining liquid collection box is communicated with the bottom of on-off control valve with described lower house by positive displacement pump.
2. automatic liquid control plant tissue liquid culture experimental provision according to claim 1, is characterized in that: described upper shell and lower house have relative flange respectively having positioning spigot circle upward in the flange of described lower house.
3. automatic liquid control plant tissue liquid culture experimental provision according to claim 2, is characterized in that: the circumferential interval of described lower house is equipped with the locating ring of predetermined altitude, and described seedling-cultivating tray has the locating hole inserting fixing pot for growing seedlings.
4. automatic liquid control plant tissue liquid culture experimental provision according to claim 3, it is characterized in that: in described lower house, liquid level sensor is housed, the signal output part of described liquid level sensor connects the controlled end of switching control valve, measuring pump, positive displacement pump, on-off control valve respectively by control circuit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520502079.8U CN204762669U (en) | 2015-07-13 | 2015-07-13 | Automatic experimental apparatus is cultivateed to accuse liquid plant tissue liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520502079.8U CN204762669U (en) | 2015-07-13 | 2015-07-13 | Automatic experimental apparatus is cultivateed to accuse liquid plant tissue liquid |
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| Publication Number | Publication Date |
|---|---|
| CN204762669U true CN204762669U (en) | 2015-11-18 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520502079.8U Expired - Fee Related CN204762669U (en) | 2015-07-13 | 2015-07-13 | Automatic experimental apparatus is cultivateed to accuse liquid plant tissue liquid |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107980640A (en) * | 2017-12-29 | 2018-05-04 | 武汉艾德士生物科技有限公司 | A kind of plant tissue culture box |
| CN110583295A (en) * | 2019-09-12 | 2019-12-20 | 江苏保丰集团公司 | Experimental device for grain is grown seedlings and is cultivated |
-
2015
- 2015-07-13 CN CN201520502079.8U patent/CN204762669U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107980640A (en) * | 2017-12-29 | 2018-05-04 | 武汉艾德士生物科技有限公司 | A kind of plant tissue culture box |
| CN110583295A (en) * | 2019-09-12 | 2019-12-20 | 江苏保丰集团公司 | Experimental device for grain is grown seedlings and is cultivated |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151118 Termination date: 20160713 |