CN204474691U - Cell culture bags special filling liquid funnel - Google Patents

Abstract

The utility model discloses a special irrigation funnel for cell culture bags, which comprises three parts: an upper inverted conical cylinder body, a middle cylindrical cylinder body and a funnel nipple at the bottom. The upper inverted conical barrel opening has a jacket crimp, and the bottom funnel nipple is centered at the bottom of the funnel. The special irrigation funnel for the cell culture bag of the utility model cooperates with the disposable cell culture bag, and is used for perfusing the cell culture liquid into the cell culture bag. The special irrigation funnel for the cell culture bag of the utility model has a simple structure and is convenient to use.

Description

Filling funnel for cell culture bag

technical field

The utility model relates to the field of medical technology, in particular to a medical material, in particular to a special irrigation funnel for cell culture bags.

Background technique

In the medical or biological industry, cell culture bags are often used for large-scale, high-density cell culture. Compared with using culture bottles, using cell culture bags to cultivate cells has many advantages: the cell culture bags are closed culture, which can be connected to PVC pipes through a sterile interface or a female-type sliding button interface for access, and needle-free injection port for needle-free injection. Bacterial cell inoculation or sampling reduces the probability of cell contamination and operator infection; the cell culture bag has a large gas exchange area, which is conducive to cultivating a large number of cells in a limited culture space, and the number of cells that can be obtained is more; the material of the cell culture bag is transparent and flexible, It is convenient to directly observe the cell state under the microscope. The disadvantage of using cell culture bags to culture cells is that it is a little inconvenient to pour the culture solution into the bag. Now, the sleeve of a 50ml syringe is used instead of the funnel, and the PVC pipe of the cell culture bag is connected to the nipple at the bottom of the sleeve. The sleeve is placed on the bracket, and the culture solution is perfused into the culture bag by gravity.

The 50ml syringe consists of a push rod, a sleeve, a rubber piston, an injection needle tube, an injection needle seat, and a needle protection cover. Bottom edge of barrel.

In the process of gravity perfusion of the culture medium with the sleeve of the 50ml syringe instead of the funnel, except for the sleeve, other parts of the syringe such as the push rod, rubber piston, injection needle tube, injection needle seat, and injection needle protective cover will be wasted. And the sleeve opening of the 50ml syringe is relatively small, and it is easy to splash outside the tube when pouring a large amount of liquid inward. In addition, the nipple of the 50ml syringe sleeve is set at the bottom edge of the sleeve instead of the bottom center of the sleeve in order to facilitate exhaust, and it is easy to retain about 1ml of liquid.

Therefore, it is necessary to develop a special filling funnel for cell culture bags in order to reduce waste and facilitate use.

Utility model content

The technical problem to be solved by the utility model is to provide a special irrigation funnel for the cell culture bag in view of the defect that the current cell culture bag does not have a special culture solution perfusion tool used in conjunction with it.

In order to solve the above technical problems, the technical solution provided by the utility model is a special filling funnel for cell culture bags, which includes three parts: an upper inverted conical cylinder, a middle cylindrical cylinder, and a funnel nipple at the bottom.

A preferred technical solution of the utility model, wherein the opening of the upper inverted conical cylinder has a curling edge.

In a preferred technical solution of the utility model, the bottom of the funnel is in an inverted conical shape, and the nipple of the funnel is located at the center of the bottom of the funnel.

In a preferred technical solution of the present invention, the material of the liquid filling funnel for the cell culture bag is medical plastic, such as PE, PP, PET, PU, etc.

In a preferred technical solution of the utility model, the wall of the filling funnel is also marked with a capacity scale.

A preferred technical solution of the utility model, the inner diameter of the opening of the upper inverted conical cylinder is 6.0 to 6.5 cm, the height of the upper inverted conical cylinder is 3.0 to 3.5 cm, the inner diameter of the middle cylindrical cylinder is 3.0 to 3.5 cm, and the height of the cylindrical cylinder is 6.5 to 3.5 cm. 7.5cm.

The advantages of the special filling liquid funnel for cell culture bags of the utility model are: it is used to perfuse cell culture liquid into the cell culture bags with a disposable cell culture bag, and consists of an upper inverted conical cylinder, a middle cylindrical cylinder and a bottom of funnel nipples. The special irrigation funnel for the cell culture bag of the utility model has a simple structure and is convenient to use.

Description of drawings

Below in conjunction with accompanying drawing and embodiment the utility model is further described:

Fig. 1 is a schematic diagram of the side view structure of the special irrigation funnel for the cell culture bag of the present invention.

Fig. 2 is a schematic diagram of the perspective structure of the special irrigation funnel for the cell culture bag of the present invention.

Fig. 3 is a top view of the special irrigation funnel for the cell culture bag of the present invention.

detailed description

The above solution will be further described below in conjunction with specific embodiments. It should be understood that these embodiments are used to illustrate the utility model and not limit the scope of the utility model. The implementation conditions used in the examples can be further adjusted according to the conditions of specific manufacturers, and the implementation conditions not indicated are usually the conditions in routine experiments.

The embodiment of the special filling funnel for cell culture bags as shown in Fig. 1, Fig. 2 and Fig. 3 includes three parts: an upper inverted conical cylinder 10, a middle cylindrical cylinder 20, and a funnel nipple 30 at the bottom, wherein The upper inverted conical barrel opening has a jacket crimp 11 , and the funnel nipple 30 is located at the center of the funnel bottom 21 .

In a preferred embodiment of the present invention, the special irrigation funnel for the cell culture bag is made of medical plastic, such as PE, PP, PET, PU, etc.

In a preferred embodiment of the present invention, the wall of the filling funnel is also marked with a capacity scale.

In a preferred embodiment of the present invention, the inner diameter of the opening of the upper inverted conical cylinder is 6.0 to 6.5 cm, the height of the upper inverted conical cylinder is 3.0 to 3.5 cm, the inner diameter of the middle cylindrical cylinder is 3.0 to 3.5 cm, and the height of the cylindrical cylinder is 6.5 cm. ~7.5cm.

This special perfusion funnel for cell culture bag is specially used for perfusing culture solution or cell suspension into the bag when the cell culture bag is used for large-scale cell culture. When in use, tear open the independent small package, take out the special filling funnel for the cell culture bag, connect the nipple at the bottom of the funnel to the PVC liquid inlet pipe of the cell culture bag, and then place the funnel on the special support frame to perform the operation of perfusing the culture solution . The special filling funnel for cell culture bags can only be used in ultra-clean benches or biological safety cabinets, and it is only for one-time use. After use, it should be disposed of as medical waste.

The special filling funnel for cell culture bags can be sterilized by ethylene oxide, sterile, non-toxic and non-pyrogenic. The special filling funnel for cell culture bags is in sterile packaging, and the product has an expiration date, and it is forbidden to use after the expiration date; it is forbidden to use when the single package is damaged or there are foreign objects; once the single package of the special filling funnel for cell culture bags is opened, use it immediately; The special irrigation funnel for the culture bag is limited to one-time use and should be destroyed after use. Operators who use the special filling funnel for this cell culture bag must have the knowledge of aseptic operation.

During transportation, the special filling funnel for cell culture bags should be protected from heavy pressure, direct sunlight, rain and snow. The special filling funnel for cell culture bags should be stored in a cool, dry, well-ventilated and clean room with a relative humidity not exceeding 80% and no corrosive gas.

The basic principles, main features and advantages of the present utility model have been shown and described above. Those skilled in the art should understand that the utility model is not limited by the above-mentioned examples. The above-mentioned examples and descriptions only illustrate the principle of the utility model. There are various changes and improvements, and these changes and improvements all fall within the scope of the claimed utility model. The scope of protection required by the utility model is defined by the appended claims and their equivalents.

Claims (5)

1. The filling funnel for cell culture bags is characterized in that it includes three parts: an upper inverted conical cylinder, a middle cylindrical cylinder, and a funnel nipple at the bottom. 2 . The filling funnel for cell culture bags according to claim 1 , characterized in that, the opening of the upper inverted conical cylinder has a jacket crimp, and the funnel nipple is located at the center of the bottom of the funnel. 3 . 3. The liquid filling funnel for cell culture bags according to claim 1, characterized in that the material of the liquid filling funnel for cell culture bags is medical plastic. 4. The special filling funnel for cell culture bags according to claim 1, characterized in that, the wall of the special filling funnel for cell culture bags is also marked with a capacity scale. 5. The special irrigation funnel for cell culture bags according to any one of claims 1-4, characterized in that the inner diameter of the opening of the upper inverted conical cylinder is 6.0-6.5 cm, and the height of the upper inverted conical cylinder is 3.0-3.5 cm. cm, the inner diameter of the cylindrical cylinder in the middle is 3.0-3.5cm, and the height of the cylindrical cylinder is 6.5-7.5cm.