CN109136076A - A kind of cell culture fluid provides device in real time - Google Patents
A kind of cell culture fluid provides device in real time Download PDFInfo
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- CN109136076A CN109136076A CN201811283820.0A CN201811283820A CN109136076A CN 109136076 A CN109136076 A CN 109136076A CN 201811283820 A CN201811283820 A CN 201811283820A CN 109136076 A CN109136076 A CN 109136076A
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- branch pipe
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- cell culture
- vial
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
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Abstract
The invention discloses a kind of cell culture fluids to provide device in real time, including pedestal and the culture bottle being set on pedestal and vial, it is disposed side by side on pedestal before and after the culture bottle and vial, the culture bottle side upper, middle and lower position has been respectively communicated with branch pipe, middle branch pipe and lower branch line, the upper branch pipe, triple valve is mounted on middle branch pipe and lower branch line, the upper branch pipe, middle branch pipe and lower branch line end, which pass through flanged joint, the Dewar bottle with cylinder valve, the Dewar bottle bottom passes through the supporting plate being arranged on culture bottle and pedestal and supports, the triple valve side passes through the liquid outlet that conduit is connected to vial, shut-off valve is mounted on the liquid outlet, the present invention can extract the cell culture fluid of culture bottle upper, middle and lower position in real time, it is conveniently used for providing the cell culture fluid of different location height in bottle, facilitate progress thin Born of the same parents detect judgement, when subsequent extraction being facilitated to provide cell culture fluid, improve and provide the quality of sample.
Description
Technical field
The invention belongs to the equipment technical field of cell culture fluid, specially a kind of cell culture fluid provides dress in real time
It sets.
Background technique
With the development of biotechnology, field of cell culture development is swift and violent.Cell culture is regenerative medicine and life science
Important branch, when cultivating cell, an important factor for device of cell culture is concerning culture cell.In the prior art,
Culture cell often to have device to have following several:
Chinese patent application 201720741150.7 discloses a kind of multi-functional cell cultivation equipment, including the first cell culture
Platform, the second cell culture table, left clapboard and right clapboard, left clapboard and right clapboard are longitudinally arranged in parallel in tray interior respectively, left
Partition, right clapboard and box side wall respectively constitute left cell culture area, middle cell culture area and right cell culture area, left cell training
It supports and is provided with the first cell cultivation groove on area's inner bottom surface, the first cell cultivation groove includes two circular grooves and cell migration slot,
Two circular grooves are connected to by cell migration slot；The second cell cultivation groove, the right side are provided in the middle cell culture area
Third cell cultivation groove is provided in cell culture area, second cell cultivation groove and third cell cultivation groove are thin with first
The structure of born of the same parents' culture tank is identical.
Chinese patent application 201520354728.4 discloses a kind of dual chamber selective penetrated property cell culture apparatus, such as Fig. 1
Shown, described device includes: at least two cell culture chamber: the first cell culture chamber and the second cell culture chamber；Wherein, described
For first cell culture chamber for cultivating the keeping cell that can secrete particular matter, second cell culture chamber is special for receiving
Substance is with training objective cell；First cell culture chamber is connected by a semi-permeable membrane with the second cell culture chamber；It is described
First cell culture chamber and the second cell culture chamber are provided with a feed liquor and inoculation channel.
In the culture of cell, cell culture fluid is both to supply cytotrophy in culture cell and germiparity is promoted to be proliferated
Basic substance, and culture cell growth and breeding living environment.When cell culture fluid detects extracting and provides, existing skill
Above-mentioned cell culture apparatus in art can not extract the cell culture fluid of upper, middle and lower position simultaneously, due to upper, middle and lower in culture bottle
The culture bottle cell state of position is different, inaccuracy when detection being caused to judge, and when subsequent progress is extracted provide again,
It extracts and residual cell culture solution is provided in the structure provided, since environmental change causes remaining culture solution to change, again
Culture solution mixing when extraction and in culture bottle influences testing result, thus so that the culture solution for extracting offer is second-rate
Cell culture fluid is caused not to be able to satisfy the requirement of cell culture.
Summary of the invention
The purpose of the present invention is to provide a kind of cell culture fluids to provide device in real time, can extract in real time on culture bottle
The cell culture fluid of lower position is conveniently used for providing the cell culture fluid of different location height in bottle, facilitates carry out cell detection
Judgement, can extract alcohol and flow through manifold configuration, carry out disinfection to branch pipe and handle and wash away remaining culture solution, after convenient
When continuous extraction provides cell culture fluid, improves and the quality of sample is provided, to solve the problems mentioned in the above background technology.
To achieve the above object, the invention provides the following technical scheme:
A kind of cell culture fluid provides device in real time, described including pedestal and the culture bottle being set on pedestal and vial
Culture bottle and vial are set on the base, the culture bottle side upper, middle and lower position be respectively communicated with branch pipe, middle branch pipe and
Lower branch line is mounted on triple valve, the upper branch pipe, middle branch pipe and lower branch line end on the upper branch pipe, middle branch pipe and lower branch line
Portion, which passes through flanged joint, the Dewar bottle with cylinder valve, and the Dewar bottle bottom passes through the branch being arranged on culture bottle and pedestal
Plate support, the triple valve side passes through the liquid outlet that conduit is connected to vial, is mounted on cut-off on the liquid outlet
Valve.
Preferably, it is arranged side by side before and after the culture bottle and vial.
Preferably, the Tissue Culture Flask and vial top position of bottleneck are combined with bottle cap.
Preferably, it is equipped with the vacuum bottleneck to match with flange at the top of the Dewar bottle, and between flange and vacuum bottleneck
It is covered with sealing ring.
Preferably, the sealing ring cross-section is positive triangle or circle, and sectional area A is 4.5-24.7mm2, material is
Silicon rubber, rebound degree τ are 50-85%.
Preferably, the liquid outlet is vertically arranged three groups above and below vial, the level height point of liquid outlet described in three groups
It is not consistent with the level height of upper branch pipe, middle branch pipe and lower branch line.
Preferably, the culture bottle and the height size of vial are consistent.
Preferably, the upper branch pipe, middle branch pipe and lower branch line shape are L shape, the upper branch pipe, middle branch pipe and lower branch line
Gasket is equipped with through position in culture bottle.
Preferably, in order to enhance sealing performance, the material of the gasket is polyvinyl chloride (PVC), thickness d 0.35-
2.5mm, elastic modulus E 3.15-3.90GPa, shore hardness Y are 50-75.Further, the elastic modulus E and shore are hard
Meet EY between degree Y and is less than or equal to 285 more than or equal to 165.When EY is less than 165, gasket is too thin or elasticity modulus too
It is low, it is unfavorable for sealing；When EY is greater than 285, the too thick the latter of gasket is too soft, is easily deformed, and leads to gasket sealing performance
Reduction.Further, the thickness d of the gasket and elastic modulus E meet: E=δ d；Wherein, δ is regulatory factor, value model
It encloses for 1.35-9.76.
Preferably, in order to make between flange and vacuum bottleneck, sealing of the culture bottle through position can satisfy and train to cell
Feeding requirement, and the degree sealed can match each other, the sectional area A of the sealing ring and thickness d, the springform of gasket
Amount E meets following relationship:
A=α·(E2·d)/2；
Wherein, α is coefficient, value range 1.12-12.5.
Compared with prior art, the beneficial effects of the present invention are:
1, cell culture fluid of the invention provides device in real time, by culture bottle upper, middle and lower position be respectively communicated with upper branch pipe, in
Branch pipe and lower branch line structure, and it is communicated with Dewar bottle in upper branch pipe, middle branch pipe and lower branch line end, it can be by opening triple valve
And cylinder valve, it is acted on using negative pressure of vacuum, the cell culture fluid of culture bottle upper, middle and lower position can be extracted in real time, be conveniently used for providing
The cell culture fluid of different location height in bottle, facilitates and carries out cell detection judgement.
2, cell culture fluid of the invention provides device in real time, can be with by the way that alcohol bottle structure is arranged in culture bottle side
One group of cell culture fluid of extraction is being provided or before extracting cell culture fluid, is opening triple valve, cylinder valve and shut-off valve, can take out
It takes alcohol to flow through manifold configuration, carries out disinfection to branch pipe and handle and wash away remaining culture solution, subsequent extraction is facilitated to provide
When cell culture fluid, improves and the quality of sample is provided.
3, cell culture fluid of the invention provides device in real time, by cross sectional shape, sectional area, material that sealing ring is arranged
Deng to improve the sealing performance between flange and vacuum bottleneck.
4, cell culture fluid of the invention provides device in real time, by the way that the thickness of gasket, elasticity modulus, hardness is arranged
Relationship between range etc. and elastic modulus E and shore hardness Y enhances sealing performance.
5, cell culture fluid of the invention provides device in real time, by sectional area A and gasket that the sealing ring is arranged
Thickness d, the relationship between elastic modulus E, make between flange and vacuum bottleneck, culture bottle can satisfy through the sealing of position
Requirement to cell culture, and the degree sealed can match each other.
Detailed description of the invention
Fig. 1 is a kind of dual chamber selective penetrated property cell culture apparatus schematic diagram in the prior art；
Fig. 2 is that cell culture fluid of the invention provides the structural schematic diagram of device in real time；
Fig. 3 is that cell culture fluid of the invention provides the overlooking structure diagram of device in real time；
Fig. 4 is that cell culture fluid of the invention provides the vial part side structure schematic view of device in real time.
In Fig. 2-4: 1 pedestal, 2 culture bottles, 3 vials, 4 bottle caps, branch pipe on 5, branch pipe, 7 lower branch lines, 8 triple valves, 9 in 6
Flange, 10 Dewar bottles, 101 cylinder valves, 11 supporting plates, 12 liquid outlets, 13 shut-off valves, 14 conduits.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description.Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Embodiment 1
Please refer to Fig. 1-3, a kind of cell culture fluid provides device in real time, including pedestal 1 and the culture bottle being set on pedestal 1
2 and vial 3, cell culture fluid is added in culture bottle 2, ethanol for disinfection is added in vial 3；The culture bottle 2 and vial 3
Front and back is disposed side by side on pedestal 1, the 2 side upper, middle and lower position of culture bottle be respectively communicated with branch pipe 5, middle branch pipe 6 and under
Branch pipe 7, triple valve 8 is mounted on the upper branch pipe 5, middle branch pipe 6 and lower branch line 7, and triple valve 8 controls upper branch pipe 5, middle branch pipe
6 and lower branch line 7 go out liquid opening and closing；The upper branch pipe 5, middle branch pipe 6 and 7 end of lower branch line pass through flange 9 and are connected with bottle
The Dewar bottle 10 of valve 101, Dewar bottle 10 can guarantee extract provide container sterile adjusting, while can be formed suction function into
Row suction；10 bottom of Dewar bottle passes through the supporting plate 11 being arranged on culture bottle 2 and pedestal 1 and supports, 8 side of triple valve
It is connected to the liquid outlet 12 of vial 3 by conduit 14, shut-off valve 13 is mounted on the liquid outlet 12, shut-off valve 13 is used
In the opening and closing of control liquid outlet 12.
The Tissue Culture Flask 2 and 3 top position of bottleneck of vial screw rear bottle cap 4；Bottle cap 4 is used for cell culture
Bottle 2 and 3 top bottleneck of vial are sealed.
It is equipped with the vacuum bottleneck to match with flange 9 at the top of the Dewar bottle 10, and is covered between flange 9 and vacuum bottleneck
There is sealing ring；It is covered with sealing ring between flange 9 and vacuum bottleneck, guarantees the leakproofness of docking location.
The liquid outlet 12 is vertically arranged three groups in about 3 vial, the level height difference of liquid outlet 12 described in three groups
It is consistent with the level height of upper branch pipe 5, middle branch pipe 6 and lower branch line 7；The level height of liquid outlet 12 respectively with upper branch pipe 5, middle branch
Pipe 6 is consistent with the level height of lower branch line 7, facilitates the docking of 14 structure of conduit.
The culture bottle 2 and the height size of vial 3 are consistent.7 shape of the upper branch pipe 5, middle branch pipe 6 and lower branch line
It is L shape, the upper branch pipe 5, middle branch pipe 6 and lower branch line 7 are equipped with gasket through position in culture bottle 2；Upper branch pipe 5, in
Branch pipe 6 and lower branch line 7 run through position in culture bottle 2 and are equipped with gasket, guarantee to run through position seals, are not in cell training
Nutrient solution leakage situation.
Structural principle: by being respectively communicated with 7 structure of upper branch pipe 5, middle branch pipe 6 and lower branch line in 2 upper, middle and lower position of culture bottle,
And be communicated with Dewar bottle 10 in upper branch pipe 5, middle branch pipe 6 and 7 end of lower branch line, can by opening triple valve 8 and cylinder valve 101,
It is acted on using negative pressure of vacuum, the cell culture fluid of 2 upper, middle and lower position of culture bottle can be extracted in real time, be conveniently used for providing in bottle not
With the cell culture fluid of position height, facilitates and carry out cell detection judgement, by the way that 3 structure of vial is arranged in 2 side of culture bottle,
One group of cell culture fluid of extraction can be provided or before extracting cell culture fluid, opening triple valve 8, cylinder valve 101 and cut-off
Valve 13 can extract alcohol and flow through manifold configuration, carry out disinfection to branch pipe and handle and wash away remaining culture solution, after convenient
When continuous extraction provides cell culture fluid, improves and the quality of sample is provided.
Embodiment 2
A kind of cell culture fluid provides device in real time, including pedestal 1 and the culture bottle being set on pedestal 12 and vial 3,
Cell culture fluid is added in culture bottle 2, ethanol for disinfection is added in vial 3；The culture bottle 2 and 3 front and back of vial are set side by side
It sets on pedestal 1, the 2 side upper, middle and lower position of culture bottle has been respectively communicated with branch pipe 5, middle branch pipe 6 and lower branch line 7, described
Triple valve 8 is mounted on upper branch pipe 5, middle branch pipe 6 and lower branch line 7, triple valve 8 controls upper branch pipe 5, middle branch pipe 6 and lower branch line 7
Go out liquid opening and closing；The upper branch pipe 5, middle branch pipe 6 and 7 end of lower branch line pass through flange 9 and are connected with the vacuum with cylinder valve 101
Bottle 10, Dewar bottle 10, which can guarantee to extract, provides the sterile adjusting of container, while can form suction function and be aspirated；It is described
10 bottom of Dewar bottle passes through the supporting plate 11 being arranged on culture bottle 2 and pedestal 1 and supports, and 8 side of triple valve passes through conduit
14 are connected to the liquid outlet 12 of vial 3, shut-off valve 13 are mounted on the liquid outlet 12, shut-off valve 13 is for controlling out liquid
The opening and closing of mouth 12.
The Tissue Culture Flask 2 and 3 top position of bottleneck of vial screw rear bottle cap 4；Bottle cap 4 is used for cell culture
Bottle 2 and 3 top bottleneck of vial are sealed.
It is equipped with the vacuum bottleneck to match with flange 9 at the top of the Dewar bottle 10, and is covered between flange 9 and vacuum bottleneck
There is sealing ring；It is covered with sealing ring between flange 9 and vacuum bottleneck, guarantees the leakproofness of docking location.
The liquid outlet 12 is vertically arranged three groups in about 3 vial, the level height difference of liquid outlet 12 described in three groups
It is consistent with the level height of upper branch pipe 5, middle branch pipe 6 and lower branch line 7；The level height of liquid outlet 12 respectively with upper branch pipe 5, middle branch
Pipe 6 is consistent with the level height of lower branch line 7, facilitates the docking of 14 structure of conduit.
The culture bottle 2 and the height size of vial 3 are consistent.7 shape of the upper branch pipe 5, middle branch pipe 6 and lower branch line
It is L shape, the upper branch pipe 5, middle branch pipe 6 and lower branch line 7 are equipped with gasket through position in culture bottle 2；Upper branch pipe 5, in
Branch pipe 6 and lower branch line 7 run through position in culture bottle 2 and are equipped with gasket, guarantee to run through position seals, are not in cell training
Nutrient solution leakage situation.
The sealing ring cross-section is positive triangle or circle, and sectional area A is 4.5-24.7mm2, material is silicon rubber,
Rebound degree τ is 50-85%.
In order to enhance sealing performance, the material of the gasket is polyvinyl chloride (PVC), thickness d 0.35-2.5mm, bullet
Property modulus E be 3.15-3.90GPa, shore hardness Y be 50-75.Further, between the elastic modulus E and shore hardness Y
Meet EY and is less than or equal to 285 more than or equal to 165.When EY is less than 165, gasket is too thin or elasticity modulus is too low, unfavorable
In sealing；When EY is greater than 285, the too thick the latter of gasket is too soft, is easily deformed, and leads to the reduction of gasket sealing performance.
Further, the thickness d of the gasket and elastic modulus E meet: E=δ d；Wherein, δ is regulatory factor, and value range is
1.35-9.76。
Embodiment 3
A kind of cell culture fluid provides device in real time, including pedestal 1 and the culture bottle being set on pedestal 12 and vial 3,
Cell culture fluid is added in culture bottle 2, ethanol for disinfection is added in vial 3；The culture bottle 2 and 3 front and back of vial are set side by side
It sets on pedestal 1, the 2 side upper, middle and lower position of culture bottle has been respectively communicated with branch pipe 5, middle branch pipe 6 and lower branch line 7, described
Triple valve 8 is mounted on upper branch pipe 5, middle branch pipe 6 and lower branch line 7, triple valve 8 controls upper branch pipe 5, middle branch pipe 6 and lower branch line 7
Go out liquid opening and closing；The upper branch pipe 5, middle branch pipe 6 and 7 end of lower branch line pass through flange 9 and are connected with the vacuum with cylinder valve 101
Bottle 10, Dewar bottle 10, which can guarantee to extract, provides the sterile adjusting of container, while can form suction function and be aspirated；It is described
10 bottom of Dewar bottle passes through the supporting plate 11 being arranged on culture bottle 2 and pedestal 1 and supports, and 8 side of triple valve passes through conduit
14 are connected to the liquid outlet 12 of vial 3, shut-off valve 13 are mounted on the liquid outlet 12, shut-off valve 13 is for controlling out liquid
The opening and closing of mouth 12.
The Tissue Culture Flask 2 and 3 top position of bottleneck of vial screw rear bottle cap 4；Bottle cap 4 is used for cell culture
Bottle 2 and 3 top bottleneck of vial are sealed.
It is equipped with the vacuum bottleneck to match with flange 9 at the top of the Dewar bottle 10, and is covered between flange 9 and vacuum bottleneck
There is sealing ring；It is covered with sealing ring between flange 9 and vacuum bottleneck, guarantees the leakproofness of docking location.
The liquid outlet 12 is vertically arranged three groups in about 3 vial, the level height difference of liquid outlet 12 described in three groups
It is consistent with the level height of upper branch pipe 5, middle branch pipe 6 and lower branch line 7；The level height of liquid outlet 12 respectively with upper branch pipe 5, middle branch
Pipe 6 is consistent with the level height of lower branch line 7, facilitates the docking of 14 structure of conduit.
The culture bottle 2 and the height size of vial 3 are consistent.7 shape of the upper branch pipe 5, middle branch pipe 6 and lower branch line
It is L shape, the upper branch pipe 5, middle branch pipe 6 and lower branch line 7 are equipped with gasket through position in culture bottle 2；Upper branch pipe 5, in
Branch pipe 6 and lower branch line 7 run through position in culture bottle 2 and are equipped with gasket, guarantee to run through position seals, are not in cell training
Nutrient solution leakage situation.
The sealing ring cross-section is positive triangle or circle, and sectional area A is 4.5-24.7mm2, material is silicon rubber,
Rebound degree τ is 50-85%.
In order to enhance sealing performance, the material of the gasket is polyvinyl chloride (PVC), thickness d 0.35-2.5mm, bullet
Property modulus E be 3.15-3.90GPa, shore hardness Y be 50-75.Further, between the elastic modulus E and shore hardness Y
Meet EY and is less than or equal to 285 more than or equal to 165.When EY is less than 165, gasket is too thin or elasticity modulus is too low, unfavorable
In sealing；When EY is greater than 285, the too thick the latter of gasket is too soft, is easily deformed, and leads to the reduction of gasket sealing performance.
Further, the thickness d of the gasket and elastic modulus E meet: E=δ d；Wherein, δ is regulatory factor, and value range is
1.35-9.76。
In order to make between flange and vacuum bottleneck, culture bottle can satisfy through the sealing of position and want to cell culture
It asks, and the degree sealed can match each other, the sectional area A and the thickness d of gasket, elastic modulus E of the sealing ring are full
It is enough lower relationship:
A=α·(E2·d)/2；
Wherein, α is coefficient, value range 1.12-12.5.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (6)
1. a kind of cell culture fluid provides device in real time, including pedestal (1) and the culture bottle (2) being set on pedestal (1) and
Vial (3), it is characterised in that: the culture bottle (2) and vial (3) are arranged on pedestal (1), the culture bottle (2) one
Side upper, middle and lower position has been respectively communicated with branch pipe (5), middle branch pipe (6) and lower branch line (7), the upper branch pipe (5), middle branch pipe (6)
With triple valve (8) are mounted on lower branch line (7), the upper branch pipe (5), middle branch pipe (6) and lower branch line (7) end pass through method
Blue (9) are connected with the Dewar bottle (10) with cylinder valve (101), Dewar bottle (10) bottom by setting in culture bottle (2) and
Supporting plate (11) support on pedestal (1), triple valve (8) side pass through the liquid out that conduit (14) is connected to vial (3)
Mouthful (12) are mounted on shut-off valve (13) on the liquid outlet (12).
2. a kind of cell culture fluid according to claim 1 provides device in real time, it is characterised in that: the Tissue Culture Flask
(2) and at the top of vial (3) position of bottleneck is combined with bottle cap (4).
3. a kind of cell culture fluid according to claim 1 or 2 provides device in real time, it is characterised in that: the Dewar bottle
(10) the vacuum bottleneck that top is equipped with and flange (9) match, and sealing ring is covered between flange (9) and vacuum bottleneck.
4. a kind of cell culture fluid according to claim 1-3 provides device in real time, it is characterised in that: it is described go out
Liquid mouth (12) is vertically arranged three groups up and down in vial (3), the level height of liquid outlet described in three groups (12) respectively with upper branch pipe
(5), middle branch pipe (6) is consistent with the level height of lower branch line (7).
5. a kind of cell culture fluid according to claim 1 provides device in real time, it is characterised in that: the culture bottle (2)
It is consistent with the height size of vial (3).
6. a kind of cell culture fluid according to claim 1 provides device in real time, it is characterised in that: the upper branch pipe (5),
Middle branch pipe (6) and lower branch line (7) run through position in culture bottle (2) and are equipped with gasket.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811283820.0A CN109136076A (en) | 2018-10-31 | 2018-10-31 | A kind of cell culture fluid provides device in real time |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811283820.0A CN109136076A (en) | 2018-10-31 | 2018-10-31 | A kind of cell culture fluid provides device in real time |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109136076A true CN109136076A (en) | 2019-01-04 |
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ID=64806741
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811283820.0A Withdrawn CN109136076A (en) | 2018-10-31 | 2018-10-31 | A kind of cell culture fluid provides device in real time |
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| Country | Link |
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| CN (1) | CN109136076A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110501202A (en) * | 2019-08-27 | 2019-11-26 | 焦作大学 | A kind of dry corrosion protected treatment device of biologic slice |
-
2018
- 2018-10-31 CN CN201811283820.0A patent/CN109136076A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110501202A (en) * | 2019-08-27 | 2019-11-26 | 焦作大学 | A kind of dry corrosion protected treatment device of biologic slice |
| CN110501202B (en) * | 2019-08-27 | 2022-02-08 | 焦作大学 | Dry anticorrosive treatment device of biological section |
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Application publication date: 20190104 |
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| WW01 | Invention patent application withdrawn after publication |