CN209537503U - A kind of Three-dimensional cell culture bottle of sustainable perfusion - Google Patents
A kind of Three-dimensional cell culture bottle of sustainable perfusion Download PDFInfo
- Publication number
- CN209537503U CN209537503U CN201821337466.0U CN201821337466U CN209537503U CN 209537503 U CN209537503 U CN 209537503U CN 201821337466 U CN201821337466 U CN 201821337466U CN 209537503 U CN209537503 U CN 209537503U
- Authority
- CN
- China
- Prior art keywords
- culture bottle
- cell culture
- step surface
- dimensional cell
- side wall
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to use for laboratory cell culture container technical fields, in particular to a kind of Three-dimensional cell culture bottle of sustainable perfusion, including cultivating bottle body, bottom is equipped with the continuous step gradually reduced in the culture bottle body, the step surface side of every level-one step is equipped with side wall, one end of the side wall opens up notch, uniformly distributed protrusion on the step surface, step surface one end of the continuous step lowest part opens up liquid storage pool, step surface where the liquid storage pool connects support plate, the other end of the support plate extends at bottle neck, the culture bottle body side wall is equipped with and above pours into mouth and lower outflux.The beneficial effects of the utility model are: creating abundant cell three-dimensional growing space, and reduce cell culture cost, the sustainable stable perfusion that can realize cell culture fluid simultaneously, ensure that the continuous stability of cells survival environment, is suitable for the growth and amplification of all kinds of attached cells.
Description
Technical field
The utility model relates to use for laboratory cell culture container technical field, in particular to the three of a kind of sustainable perfusion
Tie up Tissue Culture Flask.
Background technique
Cell culture can be used for the multiple fields such as basic research, medicament research and development and cell therapy.However most cell culture
The culture environment built of bottle is two-dimentional culture environment, can not the three-dimensional environment of accurate simulation cell in vivo, if you need to use these
Two-dimentional culture bottle carries out dimensional culture by force, needs to be added nido carrier, ball type carrier or acellular tissue matrices, and material prepares
Laborious and expensive with materials disinfection, cell is not easy elution harvest again in the carrier.And cell is needed in cell culture culture in glassware
It changes the liquid once within 1-3 days, products of cellular metabolism and concentration of glucose etc. are fluctuated, seriously affect cell state.It is therefore desirable to
Find more efficient, the more practical laboratory containers for three-dimensional cell cultivation.
Utility model content
In order to solve problems in the prior art, the utility model provides one kind and can reduce cell culture cost, can tie up
Hold the Three-dimensional cell culture bottle for stablizing sustainable perfusion of cell growing environment.
Technical solution described in the utility model is as follows:
A kind of Three-dimensional cell culture bottle of sustainable perfusion, including bottle body is cultivated, bottom in the culture bottle body
Equipped with the continuous step gradually reduced, the step surface side of every level-one step is equipped with side wall, and one end of the side wall opens up
Notch, uniformly distributed protrusion on the step surface, step surface one end of the continuous step lowest part opens up liquid storage pool, described
Step surface where liquid storage pool connects support plate, and the other end of the support plate extends at bottle neck, the bottle neck
Center line extended line and bottom surface angle it is at an acute angle, the culture bottle body side wall is equipped with and above pours into mouth and lower outflow
Mouthful, it is described on pour into mouth and lower outflux is located on the same side wall of culture bottle body, the upper filling inlet axis and company
The highest step surface middle line of continuous step, which is overlapped and liquid can be made to pour into mouth from above, smoothly to be flowed into, the dirty outlet axes and company
The continuous minimum step surface middle line of step is overlapped and liquid can be made to smoothly flow out from lower outflux.
Notch on the two neighboring side wall is arranged oppositely.
The top of the continuous step is located in culture bottle inner body wall, is passed through below the bottom end of the continuous step
Vertical plate support.
It is poured on described on mouth and lower outflux and is equipped with corking.
The top of the bottle neck is equipped with vent cap.
The minimum point of the upper step surface side wall notch of the continuous step is higher than next step surface, can make every level-one step
Can keep certain liquid level, at the same allow liquid smoothly along step from the superlative degree sequentially adequately flow through steps at different levels to
At lowermost level outflux.
The length, width and height of the protrusion are 0.1mm-5mm, each protrusion spacing 0.1mm-5mm.
The top surface of the described culture bottle body is equipped with convex edge, is embedded just below bottom surface, so as to so that
Multiple more preferable storages of culture bottle pile column.
The connection type poured between mouth, lower outflux and corking on described includes but is not limited to 6% (Rule) circular cone
Connector (standard is referring to " GB/T 1962.1-2015 " and " GB/T 1962.2-2015 ").
The culture bottle body carries out table after the techniques such as injection molding and ultrasonic bonding, using plasma technology
Face modification, enables preferably to carry positive charge, and form hydrophobic surface, and it is adherent to make it suitable for cell, is packaged in close
After closing polybag, it can be used after gamma-rays etc. carries out irradiation sterilization.
Technical solution provided by the utility model has the benefit that
By being uniformly provided with protrusion on the step surface of the continuous step of culture vessel bottom surface, total culture area is increased,
The sum that can cultivate cell is increased, to reduce cell culture cost；The setting of protrusion, cell are raw rough
Bottom surface constitutes natural culture environment, due to a part of the three-dimensional environment inherently Tissue Culture Flask of these protrusions, so
It is suitable for the operations such as the digestion elution in superclean bench, so as to preferably simulate living environment in cell body；By by
Secondary reduced continuous step and two female Luers, the power sources such as cooperation peristaltic pump are, it can be achieved that the stable pH value of cell culture, blood
Sugared concentration and nutrient concentration, cell growth state are more preferable.And since we use the continuous step gradually reduced, Ke Yishi
The sustainable stable perfusion of existing cell culture fluid, ensure that the continuous stability of cells survival environment, be suitable for all kinds of adherent thin
The growth and amplification of born of the same parents.
Detailed description of the invention
It, below will be to required in embodiment description in order to illustrate more clearly of the technical scheme in the embodiment of the utility model
Attached drawing to be used is briefly described, it should be apparent that, the accompanying drawings in the following description is only some realities of the utility model
Example is applied, it for those of ordinary skill in the art, without creative efforts, can also be according to these attached drawings
Obtain other attached drawings.
Fig. 1 is the structural schematic diagram of perspective view when the utility model removes upper top surface.
Fig. 2 is the structural schematic diagram of top view when the utility model removes upper top surface.
Fig. 3 is the portion A enlarged structure schematic diagram in the utility model Fig. 1.
Fig. 4 is the structural schematic diagram of the utility model main view；
Fig. 5 be the utility model along the sectional view of A-A；
Fig. 6 is the structural schematic diagram of the right view of the utility model；
Fig. 7 is the cross-sectional view along C-C of the utility model；
Fig. 8 is the structural schematic diagram of the utility model perspective view；
Specific embodiment
It is practical new to this below in conjunction with attached drawing to keep the purpose of this utility model, technical solution and advantage clearer
Type embodiment is described in further detail.
As shown in figures 1-8, a kind of Three-dimensional cell culture bottle of sustainable perfusion, including culture bottle body 1, the culture
Bottom is equipped with the continuous step 2 gradually reduced in bottle body 1, and 13 side of step surface of every level-one step is equipped with side wall 15, institute
The one end for the side wall 15 stated opens up notch 12, is evenly distributed with protrusion 14 on the step surface 13,2 lowest part of continuous step
13 one end of step surface opens up liquid storage pool 11, convenient for real-time detection and draws liquid in culture bottle body 1, the liquid storage pool 11
The step surface 13 at place connects support plate 4, and the other end of the support plate 4 extends at bottle neck 3, the bottle neck 3
Center line extended line and bottom surface angle it is at an acute angle, 1 side wall of culture bottle body, which is equipped with, above to be poured into mouth 21 and flows down
Outlet 22, it is described on pour into mouth 21 and lower outflux 22 is located on the same side wall of culture bottle body 1, it is described on pour into mouth
21 axis are overlapped with the highest step surface middle line of continuous step 2 and liquid can be made to pour into mouth 21 from above and smoothly flow into, under described
22 axis of outflux is overlapped with the minimum step surface middle line of continuous step 2 and liquid can be made to smoothly flow out from lower outflux 22.
Notch 12 on the two neighboring side wall 15 is arranged oppositely.
The top of the continuous step 2 is located on culture 1 inner wall of bottle body, below the bottom end of the continuous step 2
It is supported by vertical plate 5.
It is poured on described on mouth 21 and lower outflux 22 and is equipped with corking 6.
The top of the bottle neck 3 is equipped with vent cap 7.
The minimum point of the upper step surface side wall notch 12 of the continuous step 2 is higher than next step surface 13, can make each
Grade step can keep certain liquid level, while it is at different levels to adequately flow through liquid smoothly sequentially along step from the superlative degree
At step to lowermost level outflux.
The length, width and height of the protrusion 14 are 0.1mm-5mm, each protrusion spacing 0.1mm-5mm.
The top surface of the culture bottle body 1 is equipped with convex edge 8, is embedded just below bottom surface, so as to
Make multiple more preferable storages of culture bottle pile column.
The connection type poured between mouth 21, lower outflux 22 and corking 6 on described includes but is not limited to 6% (Rule)
Cone joint (standard is referring to " GB/T 1962.1-2015 " and " GB/T 1962.2-2015 "), can pass through external female Luer
It is connected with hose, and keeps sealed, sterile, hose can enters training by peristaltic pump or syringe pump, the fresh cultured of lasting infusion
The system of supporting, continual and steady nutrition and microenvironment are provided to the cell of culture.
Plasma technology can be used after the techniques such as injection molding and ultrasonic bonding in the culture bottle body 1
It is surface modified, enables preferably to carry positive charge, and form hydrophobic surface, it is adherent to make it suitable for cell, packet
After sealed plastic bag, it can be used after gamma-rays etc. carries out irradiation sterilization.
The above is only the preferred embodiment of the present invention, is not intended to limit the utility model, all practical at this
Within novel spirit and principle, any modification, equivalent replacement, improvement and so on should be included in the guarantor of the utility model
Within the scope of shield.
Claims (10)
1. a kind of Three-dimensional cell culture bottle of sustainable perfusion, including culture bottle body, which is characterized in that the culture bottle sheet
Internal bottom is equipped with the continuous step gradually reduced, and the step surface side of every level-one step is equipped with side wall, the side wall
One end opens up notch, and uniformly distributed protrusion on the step surface, step surface one end of the continuous step lowest part opens up storage liquid
Pond, the step surface where the liquid storage pool connect support plate, and the other end of the support plate extends at bottle neck, described
Bottle neck center line extended line and bottom surface angle it is at an acute angle, the culture bottle body side wall be equipped with above pour into mouth with
Lower outflux, it is described on pour into mouth and lower outflux is located on the same side wall of culture bottle body, the upper filling inlet shaft
Line is overlapped with the highest step surface middle line of continuous step and liquid can be made to pour into mouth from above and smoothly flows into, the lower outflux axis
Line is overlapped with the minimum step surface middle line of continuous step and liquid can be made to smoothly flow out from lower outflux.
2. a kind of Three-dimensional cell culture bottle of sustainable perfusion according to claim 1, which is characterized in that two neighboring institute
The notch stated on side wall is arranged oppositely.
3. a kind of Three-dimensional cell culture bottle of sustainable perfusion according to claim 1, which is characterized in that described is continuous
The top of step is located in culture bottle inner body wall, is supported below the bottom end of the continuous step by vertical plate.
4. a kind of Three-dimensional cell culture bottle of sustainable perfusion according to claim 1, which is characterized in that the upper filling
Corking is equipped on entrance and lower outflux.
5. a kind of Three-dimensional cell culture bottle of sustainable perfusion according to claim 1, which is characterized in that the bottleneck
The top of mouth is equipped with vent cap.
6. a kind of Three-dimensional cell culture bottle of sustainable perfusion according to claim 1, which is characterized in that the continuous platform
The minimum point of the upper step surface side wall notch of rank is higher than next step surface.
7. a kind of Three-dimensional cell culture bottle of sustainable perfusion according to claim 1, which is characterized in that the protrusion
Length, width and height be 0.1mm-5mm, each protrusion spacing 0.1mm-5mm.
8. a kind of Three-dimensional cell culture bottle of sustainable perfusion according to claim 1, which is characterized in that the culture
The top surface of bottle body is equipped with convex edge.
9. a kind of Three-dimensional cell culture bottle of sustainable perfusion according to claim 1, which is characterized in that the upper filling
Connection type between entrance, lower outflux and corking includes but is not limited to 6% (Rule) cone joint.
10. a kind of Three-dimensional cell culture bottle of sustainable perfusion according to claim 1, which is characterized in that the training
It supports bottle body and passes through injection molding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821337466.0U CN209537503U (en) | 2018-08-18 | 2018-08-18 | A kind of Three-dimensional cell culture bottle of sustainable perfusion |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821337466.0U CN209537503U (en) | 2018-08-18 | 2018-08-18 | A kind of Three-dimensional cell culture bottle of sustainable perfusion |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209537503U true CN209537503U (en) | 2019-10-25 |
Family
ID=68244660
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821337466.0U Active CN209537503U (en) | 2018-08-18 | 2018-08-18 | A kind of Three-dimensional cell culture bottle of sustainable perfusion |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209537503U (en) |
-
2018
- 2018-08-18 CN CN201821337466.0U patent/CN209537503U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA1270467A (en) | System for metering nutrient media to cell culture containers and method | |
| ES2598055T3 (en) | Device for aseptic cell expansion | |
| CN110835602A (en) | Three-dimensional cell culture bottle capable of realizing continuous perfusion | |
| CN103614296B (en) | Double-chamber three-dimensional pouring bioreactor system | |
| CN102399693B (en) | Simulation three-dimensional cell cultivation device and cultivation method | |
| CN106676005A (en) | Culture vessel for cell culture and cell sorting | |
| CN204644371U (en) | A kind of integrated form many cells noncontact co-culture device | |
| CN207276632U (en) | Disposable Tissue Culture Flask | |
| CN103131635B (en) | Cavity-type dynamic-filling bioreaction device | |
| CN209537503U (en) | A kind of Three-dimensional cell culture bottle of sustainable perfusion | |
| CN106497784A (en) | A kind of control pressurer system and its compress control method for realizing culture fluid and waste liquid exchange | |
| CN207193299U (en) | One kind culture DC CIK cell blake bottles | |
| CN207294804U (en) | The high efficiency cell blake bottle of built-in disturbing flow device | |
| CN207047204U (en) | A kind of Enrichment by Microorganisms acquisition means | |
| CN108504574A (en) | Culture bottle | |
| CN202081110U (en) | Constant concentration cell culture device with micropump control function | |
| CN204443239U (en) | A kind of Cordyceps militaris planting pot | |
| CN204874544U (en) | Dynamic perfusion culture system of external promotion organizational project product vascularization | |
| CN204162724U (en) | A kind of perfusion cap cultivated for biological sample filling type | |
| CN2928694Y (en) | North Chinese caterpillar fungus wine bottle | |
| CN208378905U (en) | A kind of cell co-cultivation unit | |
| CN207130275U (en) | A kind of co-culture device | |
| CN206879566U (en) | A kind of multi-functional Chinese herbal medicine seedlings nursing plate | |
| CN206902165U (en) | A kind of high efficiency culture systems of mass cell production and culture | |
| CN201971839U (en) | Potential difference circulatory bioreactor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |