Disposable Tissue Culture Flask
Technical field
Biological technical field is the utility model is related to, it particularly relates to the cell culture in technical field of cell culture
Utensil, espespecially disposable Tissue Culture Flask.
Background technology
Tissue Culture Flask is the common vessel in cell culture, it can be divided into glass, plastics etc. according to material;The bottom of according to
Portion's shape can be divided into square, triangle, circle etc.;It can be divided into closed, permeability type etc. according to different cell culture demands;
It can be divided into all kinds such as 5mL to 500mL according to volume;It can be divided into square bottom of bottle, rectangle according to the shape of bottle body bottom
Bottom of bottle, triangle bottom of bottle etc.;Straight neck, torticollis, angle neck, triangle neck, rectangle can be divided into according to the bottleneck of blake bottle bottle
Neck etc..In addition, cell culture bottle surface after special modification, can allow cell preferably to carry out adhere-wall culture.
The design of existing disposable plastic blake bottle is that circular bottleneck and square bottle are directly docked, this is just
Their junction is caused the corner angle to raise up occur, during liquid pouring, always having some liquid cannot be complete
It is complete to topple over totally.The experimental implementation abandoned is drawn using suction pipe progress remaining liq this adds increased a step.This operation is increasing
While having added experimental work amount, a chance of cell contamination is added.
Prior art situation, notification number CN203403093U disclose a kind of Tissue Culture Flask, it has the technical effect that:
Cell attachment dielectric layer is added in the inner cavity of the bottle of Tissue Culture Flask, cell patch can be carried out by adding in Tissue Culture Flask
The surface area of wall culture, in cell cultivation process, adherent density increase including cell, improves Tissue Culture Flask and once cultivates carefully
The amount of born of the same parents.
Notification number CN 202369595U, disclose a kind of Tissue Culture Flask, diligent with cell culture and observation two
Energy.By pellicle, be conducive to cell ventilation, meet the needs of oxygen and carbon dioxide, be conducive to cell growth, cell Proliferation
It hurry up, culture effect is good;Pellicle can effectively prevent external microbe from entering blake bottle, pollution-free in bottle, make growing, being numerous for cell
Grow at one's best, cell viability height.
Notification number CN203419929U, discloses the bottle of Tissue Culture Flask, realizes that multiple Tissue Culture Flasks keep flat overlapping,
Storage and movement can be stacked, saves incubator space, convenient management;Upside plate face is provided with frosting, easy to marking pen mark
Note;The upper end of body is provided with bottleneck, and bottleneck is tilted towards rear panel, and the downside of bottleneck is arc surface structure.Blake bottle has
Certain angle of inclination, it is convenient to harvest cell, avoid blake bottle from producing and be not easy to extract the extraction dead angle of cell.
Notification number CN203513696U, discloses Tissue Culture Flask, solves multiple Tissue Culture Flasks and does not allow manageability, deposits
The problem of putting and be difficult to extract with Tissue Culture Flask generally existing, and easily producing extraction dead angle.
Notification number CN204676090U, discloses antifog Tissue Culture Flask, by setting heating unit in blake bottle bottom,
Set heating controller to be configured the heating-up temperature of heating unit outside heating unit, blake bottle bottom is added
Heat, remains unchanged the steam temperature in bottle, prevents vapor to be internally formed water mist in blake bottle roof.
Notification number CN204918617U, discloses a kind of Tissue Culture Flask, can aseptically realize good breathe freely
Property, and can to avoid culture medium excessive vaporization, whole Tissue Culture Flask can through thoroughly cleaning, Reusability after autoclaving,
Greatly reduce suspension cell culture cost, the bio-pharmaceutical development and exploitation to be prepared using suspension cell culture provide through
The cell culture pattern of Ji.
Notification number CN204803322U, discloses novel cell culture rolling bottle, the novel cell culture rolling bottle of utility model,
Bottle cap coordinates with bottleneck thread so that can be sealed inside bottle, it is ventilative in conveniently regulating and controlling bottle when bottle needs ventilative
Rate, while bottle inner cell culture will not revealed, and will not cross contamination, cell culture effect is more preferable.
Notification number CN204803326U, discloses cell-culturing rotating bottle, and air hole and ventilated membrane are set on bottle cap,
Guarantee, which screws bottle cap, to be prevented nutrient solution from leaking hunting, prevents from while cross contamination gas being come in and gone out in bottle, cultivates cell
Being capable of normal growth.
Notification number CN204958945U, discloses a kind of disposable Tissue Culture Flask for being provided with intake chute, this practicality is new
The intake chute of smooth arc is provided with the top of the bottle of the disposable Tissue Culture Flask of type between bottleneck opening, so that thin
The all liq in cell bottle is toppled over by energy once-through operation completely when born of the same parents' top of bottle carries out liquid pouring and is finished, avoids use
Suction pipe carries out the secondary operation inhaled and abandoned, and considerably increases the convenience of Cell Lab operation.
Notification number CN204981898U, discloses Tissue Culture Flask, if will be generally incubated appearance by the parallel sieve plate of dried layer
Cell culture chamber in device is separated, and reunion propagation the arriving on 3D directions of cell suppresses, and is intended to institute between sieve plate
The in-plane expansion of clamping, forms the tiling growth pattern of tablet culture.
Notification number CN205188281U, discloses Tissue Culture Flask, by setting liquid exchange opening on bottle inspection surface so that
During cell culture, it can directly carry out changing liquid by the liquid exchange opening, without tilting bottle, so as to avoid because tilting bottle, cell
The problem of departing from bottle wall under the effect of gravity, is more advantageous to the adherence quality growth of cell.
Notification number CN205170865U, discloses a kind of sheet cell culture vector and Tissue Culture Flask, using this practicality
New sheet cell culture vector and blake bottle cultivate anchorage-dependent cells, and culture carrier suspends in cell culture fluid
Property it is good, seldom occur viscous glutinous, so as to greatly improve bond area, be very suitable for concentration cultivation.
Notification number CN206014951U, discloses a kind of attached cell blake bottle, the attached cell culture of the utility model
Bottle, when collecting cell, can open top plate, easy to operate by the open ports adherent cell collecting of back box, shorten adherent thin
Born of the same parents' acquisition time, improves work efficiency.
Notification number CN205839034U, discloses Tissue Culture Flask, by rotating tune of the second lid realization to air hole
Section, improves the gas permeability of bottle cap;First lid effectively reduces what is be infected with germs when bottle cap is positioned on operation console in setting is renovated
Probability, there is provided one more sterile environment of cell culture.
Notification number CN205839035U, discloses cell culture fluid storage bottle, including body, is tiltedly installed with and leads on body
Stream portion, diversion division and body junction are in arc-shaped, and seal is connected with diversion division.When dispensing cell culture fluid, open close
Sealing, topples over downwards along the incline direction of diversion division, and diversion division and body junction are in arc-shaped, easy to the training inside body
Nutrient solution is trickled along the junction of arc-shaped to diversion division, flows out cell culture fluid storage bottle.Since the connection of arc-shaped is in
Inclined diversion division has the guiding role more relaxed to cell culture fluid, even if the cell culture liquid hold-up inside storage bottle
When less, nutrient solution is also easier to be dumped out.
Notification number CN206328407U, discloses a kind of Tissue Culture Flask, including cell culture bottle body and sucker, bottle
On be provided with screw hole, the body of rod is provided with sucker, there is the external screw thread to match with screw hole thereon, the body of rod is screwed into screw hole, makes training
Support bottle and place and stablize.
Notification number CN203683525U, discloses the cell-enriching device based on Selective cell retention technology, the present apparatus
Make cell suspension flow with adhesion material to contact, improve the adherence rate of cell and adhesion material in cell suspension,
Syringe injection and the speed drawn are controlled by the speed control unit on host, so as to control cell suspension in enrichment chamber
And the flowing velocity in circulation line, targetedly preferential compound inhomogeneous cell, its is simple in structure, easy to operate.
Above-mentioned technical proposal improves and optimizates Tissue Culture Flask from different perspectives, but leads to for the cells containing sequences of mixed culture
Nature culture is crossed, separation obtains different types of cell, still do not provide good technical solution, therefore, the present invention carries
The following content of the invention is gone out.
Utility model content
For defect existing in the prior art, the purpose of the utility model is to overcome the drawbacks described above of the prior art,
There is provided one kind can disposably topple over totally, so as to reduce pollution, the disposable cell without using suction pipe processing remaining liq
Blake bottle.
To achieve the above objectives, the technical scheme adopted by the utility model is that:
Disposable Tissue Culture Flask, including blake bottle bottle 7 and bottleneck 3;Bottleneck 3 is connected to the left side of blake bottle bottle 7,
And tilted to the upper left side of blake bottle bottle 7, the bottleneck 3 is connected with bottle cap 1;Bottle cap 1 is equipped with the arc drain in shrinkage type
Groove, anti-skidding scale texture 2 is set on the side wall of bottleneck 3, bottle card stupefied 13 is fixed with the outside of the opening of bottleneck 3;Blake bottle bottle
The bottom of body 7 is equipped with some elastic cushions 5, and the right side wall of blake bottle bottle 7 is equipped with outwardly directed chase 6, blake bottle bottle 7
Internal bottom is equipped with some shelvings 4.
Further, the material of the disposable Tissue Culture Flask can select the various materials of the prior art, such as glass
Glass or plastics, preferably plastic bottle, such as polypropylene vial, polyethylene bottle.
Further, the volume of the blake bottle bottle 7 is 10mL to 250mL.
Further, the quantity of the elastic cushion 5 is 4, is distributed at the bottom four corners of blake bottle bottle 7;It is described
The quantity of shelving 4 is 6, the bottom being placed in parallel in inside blake bottle bottle 7, and it is to expand the disposable cell training that it, which is acted on,
The floor space of bottle is supported, improves the efficiency of cell culture.
Further, the section of the shelving 4 is triangle, highly in 0.5-1cm.
Further, the section of the chase 6 is triangle, and 6 side of chase is high, and side is low, makes liquid to a side
To flowing, easy to the absorption and collection of liquid.
Further, the elastic cushion 5 is dismountable rubber structure, is elasticity at the top center of elastic cushion 5
Cushion suction inlet 9,9 periphery of elastic cushion suction inlet are elasticoviscous property rubber cushion 10, are reached by the air for extruding 5 chassis interior of elastic cushion
Negative pressure state, is close to the bottom of blake bottle bottle 7.
Further, the bottle cap 1 is the contractile elastomeric cap made by polyethylene rubber, and the side of bottle cap 1
Equipped with bottle cap bayonet 12, opposite side is equipped with bottle cap and bottleneck connector 11, and bottle cap bayonet 12 fixes bottle cap by bottle card stupefied 13
1, bottle cap 1 is connected with bottleneck 3 by bottle cap with bottleneck connector 11.
Further, opening is arranged at the top of the blake bottle bottle 7, and the area of opening accounts for the 3/4 of upper surface area, opening
Place is equipped with upper cover 8, and the upper cover 8 is connected by card slot with blake bottle bottle 7.
Further, the upper cover 8 is equipped with breathable films, prevents bacterium and cell entry.
The utility model is in arc from bottle cap direction to bottleneck direction by setting anti-skidding scale texture on the side wall of bottleneck
The bottle cap of shape has the function of cork, so as to will not expose when Tissue Culture Flask carries out liquid pouring, blake bottle bottle body bottom has 4
A elastic cushion, prevents Tissue Culture Flask is placed from toppling over and breakking away when being superimposed, the triangle chase side height of Tissue Culture Flask,
Side is low, is collected easy to liquid, is stamped fungi-proofing and anti-virus breathable films thereon, can ensure diplomatic in gas large area
Change, maintain cell nutrients;There is shelving inside Tissue Culture Flask, increase the culture area of attached cell, improve culture efficiency, promote
Made full use of into nutrient.
The utility model considerably increases the convenience of Cell Lab operation, also, the presence of intake chute can also one
Determine reduction culturing gene in degree to rock and flow in bottle cap, pollution is reduced, easy to draw liquid;Due to shrinkage type arc drain
The presence of groove, the area of blake bottle bottle body bottom is relatively still bigger, thereby may be ensured that larger culture face, saves empty
Between, avoid culture medium from splashing easily on bottle cap.
Brief description of the drawings
The utility model has drawings described below:
Fig. 1 is the overall structure figure (front view) of the disposable Tissue Culture Flask of the utility model,
Fig. 2 is the structure chart of the elastic cushion in Fig. 1,
Fig. 3 is the structure chart of blake bottle bottle body bottom,
Fig. 4 is the structure chart of the bottle cap in Fig. 1.
Wherein 1, bottle cap, 2, scale texture, 3, bottleneck, 4, shelving, 5, elastic cushion, 6, chase, 7, blake bottle bottle, 8,
Upper cover, 9, elastic cushion suction inlet, 10, elasticoviscous property rubber cushion, 11, bottle cap and bottleneck connector, 12, bottle cap bayonet, 13, bottle card
It is stupefied.
Embodiment
The utility model is described in further detail below in conjunction with attached drawing 1-4.
Following embodiments further illustrate the content of the utility model, but should not be construed as the limitation to the utility model.
In the case of without departing substantially from the utility model spirit and essence, the modification made to the utility model method, step or condition or
Replace, belong to the scope of the utility model.
Unless otherwise specified, the conventional means that technological means used in embodiment is well known to those skilled in the art.
As shown in Figs 1-4, disposable Tissue Culture Flask described in the utility model, including blake bottle bottle 7 and bottleneck 3;
Bottleneck 3 is connected to the left side of blake bottle bottle 7, and is tilted to the upper left side of blake bottle bottle 7, and the bottleneck 3 connects with bottle cap 1
Connect;Bottle cap 1 is equipped with the arc intake chute in shrinkage type, and anti-skidding scale texture 2, the opening of bottleneck 3 are set on the side wall of bottleneck 3
Outside is fixed with bottle card stupefied 13;The bottom of blake bottle bottle 7 is equipped with some elastic cushions 5, and the right side wall of blake bottle bottle 7 is set
There is an outwardly directed chase 6, the bottom inside blake bottle bottle 7 is equipped with some shelvings 4.
Further, the material of the disposable Tissue Culture Flask can select the various materials of the prior art, such as glass
Glass or plastics, preferably plastic bottle, such as polypropylene vial, polyethylene bottle.
Further, the volume of the blake bottle bottle 7 is 10mL to 250mL.
Further, the quantity of the elastic cushion 5 is 4, is distributed at the bottom four corners of blake bottle bottle 7;It is described
The quantity of shelving 4 is 6, the bottom being placed in parallel in inside blake bottle bottle 7, and it is to expand the disposable cell training that it, which is acted on,
The floor space of bottle is supported, improves the efficiency of cell culture.
Further, the section of the shelving 4 is triangle, highly in 0.5-1cm.
Further, the section of the chase 6 is triangle, and 6 side of chase is high, and side is low, makes liquid to a side
To flowing, easy to the absorption and collection of liquid.
Further, the elastic cushion 5 is dismountable rubber structure, is elasticity at the top center of elastic cushion 5
Cushion suction inlet 9,9 periphery of elastic cushion suction inlet are elasticoviscous property rubber cushion 10, are reached by the air for extruding 5 chassis interior of elastic cushion
Negative pressure state, is close to the bottom of blake bottle bottle 7.
Further, the bottle cap 1 is the contractile elastomeric cap made by polyethylene rubber, and the side of bottle cap 1
Equipped with bottle cap bayonet 12, opposite side is equipped with bottle cap and bottleneck connector 11, and bottle cap bayonet 12 fixes bottle cap by bottle card stupefied 13
1, bottle cap 1 is connected with bottleneck 3 by bottle cap with bottleneck connector 11.
Further, opening is arranged at the top of the blake bottle bottle 7, and the area of opening accounts for the 3/4 of upper surface area, opening
Place is equipped with upper cover 8, and the upper cover 8 is connected by card slot with blake bottle bottle 7.
Further, the upper cover 8 is equipped with breathable films, prevents bacterium and cell entry.
Embodiment 1
Exemplified by cultivating hepatoma Hep G 2 cells, illustrate, cell culture fluid chooses 1640 culture mediums, before the test, will
Cultivate cell to thaw, recovery, while prepare cell culture fluid 500ml, pipette, Tissue Culture Dish, Tissue Culture Flask are passed through
Autoclaving sterilization, puts superclean bench into after alcohol wipe sterilizes, proceeds as follows:
In superclean bench, 4 elastic cushions are respectively installed to the bottom of Tissue Culture Flask, then open upper cover 8,
Whether the breathable films of observation upper cover 8 have damaged, if having there is rupture, replace in time, continue to test if without damage.
First the distilled water of 300ml is imported into Tissue Culture Flask from upper cover 8, is jiggled, then by it at bottleneck 3
Opening export, so repeatedly 2 times, finally with after the cell culture fluid rinse Tissue Culture Flask of 100ml, it is opened at bottleneck 3
After mouthful export and cover bottle cap 1.
Then the cell culture fluid of 100ml is moved into Tissue Culture Flask at upper cover 8, then will have been recovered with pipette
Liver cancer cells are open through upper cover 8 and are moved into Tissue Culture Flask, allow glass pipette to blow and beat cell repeatedly, avoid its skewness, blow
After beating, bottle cap 1 is fastened, covers upper cover 8.Be put into cell incubator, continue culture 24 it is small when, 24 it is small when after, take out
Tissue Culture Flask, carries out changing liquid.
Tissue Culture Flask after alcohol volatilization, then is first opened into Tissue Culture Flask with 75% alcohol wipe outer surface
Bottle cap 1, extracts its liquid, is then shut off the bottle cap 1 of Tissue Culture Flask.
Then, upper cover 8 is opened, clean liquid is not drawn by remaining, using Tissue Culture Flask is tilted, enters liquid
Chase 6, is exhausted liquid with pipettor, and the nutrient solution 100ml newly prepared is moved into cell training by the upper cover 8 of Tissue Culture Flask
Support in bottle, then shut the upper cover 8 of Tissue Culture Flask, Tissue Culture Flask is put back in cell incubator.
The content not being described in detail in this specification belongs to the prior art known to professional and technical personnel in the field.