CN203403093U - Cell culture bottle - Google Patents
Cell culture bottle Download PDFInfo
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- CN203403093U CN203403093U CN201320331854.9U CN201320331854U CN203403093U CN 203403093 U CN203403093 U CN 203403093U CN 201320331854 U CN201320331854 U CN 201320331854U CN 203403093 U CN203403093 U CN 203403093U
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- Prior art keywords
- bottle
- tissue culture
- culture flask
- cell
- woven fabrics
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Abstract
The utility model discloses a cell culture bottle in the field of medical equipment. The cell culture bottle comprises a bottle body and a bottle cap, wherein the bottle cap is fixed on a bottle mouth of the bottle body; a ring-shaped cell adherence dielectric layer is fixed in an inner cavity of the bottle body; the cell adherence dielectric layer is separated from the inner wall of the bottle body. The cell culture bottle has the technical effects that the cell adherence dielectric layer is added into the inner cavity of the bottle body of the cell culture bottle, so that the surface area for cell adherence culture in the cell culture bottle can be enlarged, and therefore, the cell adherence density in the cell culture bottle is increased in the cell culture process, and the one-time cell culture quantity of the cell culture bottle can be increased.
Description
Technical field
The utility model relates to a kind of Tissue Culture Flask in medical equipment field.
Background technology
Tissue Culture Flask is the important equipment that carries out drug test and implantable medical devices test, and the stopping property of Tissue Culture Flask and security are quite high, thereby are subject to pharmaceutical industries and medical device industry researchist's extensive favor.Tissue Culture Flask can be divided into according to material: glass Tissue Culture Flask and plastic cell culture bottle, the shape of Tissue Culture Flask bottom can be circle, square, rectangle, trilateral etc.The volume of Tissue Culture Flask has the plurality of specifications such as 500ml to 3L.The adherent area of Tissue Culture Flask, the surface-area that can carry out cell attachment cultivation in Tissue Culture Flask, comprises 275cm
2～3000cm
2plurality of specifications.
Yet current Tissue Culture Flask all exists a technical bottleneck, it is the requirement that the amount of a culturing cell of Tissue Culture Flask cannot meet experiment, therefore need more than one Tissue Culture Flask to carry out cell cultures, cause the increase of cost in medicine and medicine equipment R&D process, the waste of resource.
Cause the reason of the problems referred to above to be: the amount that mainly improves at present a culturing cell of Tissue Culture Flask by increasing the volume of Tissue Culture Flask.But this thinking can not improve the amount of a culturing cell of Tissue Culture Flask safely, and the sealing property of Tissue Culture Flask also may significantly reduce, in cell cultivation process, contaminated risk strengthens.
Utility model content
The purpose of this utility model is in order to overcome the deficiencies in the prior art, and a kind of Tissue Culture Flask is provided, and it can improve in cell cultivation process, cell adherent density in Tissue Culture Flask, thus improve the amount of a culturing cell of Tissue Culture Flask.
A kind of technical scheme that realizes above-mentioned purpose is: a kind of Tissue Culture Flask, comprise bottle and bottle cap, and described bottle cap is fixed on the bottleneck of described bottle;
In the inner chamber of described bottle, be fixed with annular cell attachment medium layer, described cell attachment medium layer is separated with the inwall of described bottle.
Further, described cell attachment medium layer is non-woven fabrics plicated layer.
Further, the center of the intracavity bottom of described bottle is provided with projection, and described non-woven fabrics plicated layer is radially fixing in the inner chamber of described bottle.
Further, the thickness of the non-woven fabrics that described non-woven fabrics plicated layer is used is 2～3mm, and is formed by trevira braiding, and the diameter of described trevira is 20～30 μ m.
Further, vacuum plasma surface treatment is all passed through on the two sides of the inwall of described bottle and described non-woven fabrics plicated layer.
Further, described bottle cap is a kind of in closed cover, polyester lid, ventilating cover and dottle pin lid.
Further, described bottle is made by glass.
Further, described bottle is made by borosilicate glass.
Further, described bottle is made of plastics.
Further, described bottle is made by polystyrene or polyester.
Adopt the technical scheme of a kind of Tissue Culture Flask of the present utility model,, in the inner chamber of the bottle of Tissue Culture Flask, be fixed with annular cell attachment medium layer, the technical scheme that described cell attachment medium layer is separated with the inwall of described bottle.Its technique effect is: in the inner chamber due to the bottle at Tissue Culture Flask, increased cell attachment medium layer, increased and can carry out the surface-area of cell attachment cultivation in Tissue Culture Flask, therefore, in cell cultivation process, cell adherent density in Tissue Culture Flask increases, thereby improves the amount of a culturing cell of Tissue Culture Flask.
Accompanying drawing explanation
Fig. 1 is the front view of a kind of Tissue Culture Flask of the present utility model
Fig. 2 is that the A-A of Fig. 1 is to view.
Embodiment
Refer to Fig. 1 and Fig. 2, contriver of the present utility model is in order to understand the technical solution of the utility model better, below by embodiment particularly, and is elaborated by reference to the accompanying drawings:
Refer to Fig. 1, a kind of Tissue Culture Flask of the present utility model, comprises bottle 1 and bottle cap 2, and bottle cap 2 is fixed on the bottleneck 11 of bottle 1, to prevent that the cell in Tissue Culture Flask from being polluted.
In the inner chamber 12 of bottle 1, be fixed with annular cell attachment medium layer 3, the bottom of the inner chamber 12 of the bottom of cell attachment medium layer 3 and bottle 1 is fixed, and cell attachment medium layer 3 is separated with the inwall of bottle 1.
The object of design is like this: the inner and outer wall of cell attachment medium layer 3 all can carry out cell attachment cultivation, like this, the surface-area that can carry out cell attachment cultivation in Tissue Culture Flask at least increases to original three times, in cell cultivation process, cell adherent density in Tissue Culture Flask increases to original three times, and the amount of a culturing cell of Tissue Culture Flask can increase to original three times.Therefore, the amount of a culturing cell of Tissue Culture Flask can meet the requirement of experiment, cost in medicine and medicine equipment R&D process, and the loss of resource reduces.
In the present embodiment, in the inner chamber 12 of the bottle 1 of Tissue Culture Flask, what cell attachment medium layer 3 adopted is non-woven fabrics plicated layer.The described non-woven fabrics plicated layer that cell attachment medium layer 3 adopts non-woven fabrics to make further increases the surface-area that can carry out cell attachment cultivation in Tissue Culture Flask.Adopt after described non-woven fabrics plicated layer, the surface-area that can carry out cell attachment cultivation in Tissue Culture Flask at least increases to original five to seven times, in cell cultivation process, cell adherent density in Tissue Culture Flask increases to original five to seven times, and the amount of a culturing cell of Tissue Culture Flask can increase to original five to seven times.The amount of a culturing cell of Tissue Culture Flask can further meet the requirement of experiment, and in medicine and medicine equipment R&D process, cost further reduces, and the loss of resource reduces.
Adopt the another one reason of described non-woven fabrics plicated layer to be: the perviousness of non-woven fabrics and permeability are all fine, can guarantee the inner and outer wall at described non-woven fabrics plicated layer, and the supply of the required nutriment of adherent Growth of Cells and/or oxygen can obtain well and meets on the inwall of bottle 1, the surviving rate of cell in culturing process obtained significant raising.
In the present embodiment, described non-woven fabrics plicated layer preferred polyester fabric nonwoven cloth, because its cellular affinity is good, the thickness of polyester fiber non-woven fabric is 2～3mm preferably, and such thickness can effectively be guaranteed described non-woven fabrics plicated layer structural stability, mechanical property, perviousness and ventilation property.Described polyester fiber non-woven fabric is that the trevira braiding that is 20～30 μ m by diameter forms.Select the object of trevira in this to be: described non-woven fabrics plicated layer structural stability, mechanical property, perviousness and ventilation property can effectively be guaranteed.
In order to guarantee the structural stability of described non-woven fabrics plicated layer, the center of inner chamber 12 bottoms of bottle 1 is provided with projection 13, and described non-woven fabrics plicated layer is radially fixing in the inner chamber 12 of bottle 1.
The bottle cap 2 of Tissue Culture Flask mainly contains at present: closed cover, polyester lid, ventilating cover, dottle pin lid etc.
Interior pad is not with in the bottom of described closed cover, for the airtight cultivation of cell, guarantees the stopping property of Tissue Culture Flask in cell cultivation process.
Described polyester lid is usually used in open cell cultures, adopting Tissue Culture Flask to come in the process of culturing cell, only need unscrew gently described polyester lid, just can make gas in Tissue Culture Flask and the gas in environment exchange, meanwhile, the described non-woven fabrics plicated layer that described polyester lid is also easy to be woven into trevira is fixed.
The bottom of described ventilating cover has increased the hydrophobic filter membrane (not shown) of 0.2 μ m, to provide aseptic gas exchange for carrying out cultured cells in Tissue Culture Flask, reduces the contaminated risk of cell in Tissue Culture Flask.Use the Tissue Culture Flask of described ventilating cover, be usually used in the open culture of cell, be particluarly suitable for CO
2incubator carries out cell cultures, is particularly useful for carrying out the experiment of long term cell culture.
The bottom of described dottle pin lid has increased dottle pin (not shown), the top of described dottle pin and described non-woven fabrics plicated layer is fixed, on described dottle pin with precut mouth, can make suction nozzle like this, or the transfer pipet of the following specification of 5ml thrusts this dottle pin, Tissue Culture Flask is carried out to the operation of liquid feeding, imbibition or harvested cell, reduce the contaminated chance of cell in cell dispensing bottle, maintain Tissue Culture Flask inner sealing sterile environment.
The angle consideration of cultivating from cell attachment, the material of the bottle 1 of Tissue Culture Flask can be selected glass or plastics.In the present embodiment, the preferred borosilicate glass of bottle 1, polystyrene or the polyester of Tissue Culture Flask.Borosilicate glass, polyester or polystyrene are good to the affinity of cell on the one hand, and one side corrosion resistance and good, is beneficial to cell in the cultivation of Tissue Culture Flask, especially long-term cultivation in addition.
Simultaneously, the inwall of bottle 1, and the inner and outer wall of described non-woven fabrics plicated layer all can pass through special surface modification, the inwall of the bottle 1 further improving, and the cellular affinity of the inner and outer wall of described non-woven fabrics plicated layer, be convenient to carry out more cellulous adherent culture, simultaneously cell the two sides of the inwall of bottle 1 and described non-woven fabrics plicated layer breed with adherent after, relatively easily digest.Described special surface modification comprises vacuum plasma surface treatment etc.
Claims (10)
1. a Tissue Culture Flask, comprises bottle (1) and bottle cap (2), and described bottle cap (2) is fixed on the bottleneck (11) of described bottle (1), it is characterized in that:
In the inner chamber (12) of described bottle (1), be fixed with annular cell attachment medium layer (3), described cell attachment medium layer (3) is separated with the inwall of described bottle (1).
2. a kind of Tissue Culture Flask according to claim 1, is characterized in that: described cell attachment medium layer (3) is non-woven fabrics plicated layer.
3. a kind of Tissue Culture Flask according to claim 2, is characterized in that: the center of inner chamber (12) bottom of described bottle (1) is provided with projection (13), and described non-woven fabrics plicated layer is radially fixing in the inner chamber (12) of described bottle (1).
4. according to a kind of Tissue Culture Flask described in any one in claims 1 to 3, it is characterized in that: a kind of in closed cover, polyester lid, ventilating cover and dottle pin lid of described bottle cap (2).
5. according to a kind of Tissue Culture Flask described in any one in claims 1 to 3, it is characterized in that: described bottle (1) is made by glass.
6. a kind of Tissue Culture Flask according to claim 5, is characterized in that: described bottle (1) is made by borosilicate glass.
7. according to a kind of Tissue Culture Flask described in any one in claims 1 to 3, it is characterized in that: described bottle (1) is made of plastics.
8. a kind of Tissue Culture Flask according to claim 7, is characterized in that: described bottle (1) is made by polystyrene or polyester.
9. according to a kind of Tissue Culture Flask described in claim 2 or 3, it is characterized in that: the thickness of the non-woven fabrics that described non-woven fabrics plicated layer is used is 2～3mm, and formed by trevira braiding, the diameter of described trevira is 20～30 μ m.
10. according to a kind of Tissue Culture Flask described in claim 2 or 3, it is characterized in that: vacuum plasma surface treatment is all passed through on the inwall of described bottle (1) and the two sides of described non-woven fabrics plicated layer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320331854.9U CN203403093U (en) | 2013-06-08 | 2013-06-08 | Cell culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320331854.9U CN203403093U (en) | 2013-06-08 | 2013-06-08 | Cell culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203403093U true CN203403093U (en) | 2014-01-22 |
Family
ID=49939031
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320331854.9U Expired - Fee Related CN203403093U (en) | 2013-06-08 | 2013-06-08 | Cell culture bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203403093U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103289897A (en) * | 2013-06-08 | 2013-09-11 | 上海日泰医药设备工程有限公司 | Cell culture bottle |
-
2013
- 2013-06-08 CN CN201320331854.9U patent/CN203403093U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103289897A (en) * | 2013-06-08 | 2013-09-11 | 上海日泰医药设备工程有限公司 | Cell culture bottle |
| CN103289897B (en) * | 2013-06-08 | 2014-11-19 | 上海日泰医药设备工程有限公司 | Cell culture bottle |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140122 Termination date: 20210608 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |