CN205368399U - Cell culture bottle - Google Patents
Cell culture bottle Download PDFInfo
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- CN205368399U CN205368399U CN201620109949.XU CN201620109949U CN205368399U CN 205368399 U CN205368399 U CN 205368399U CN 201620109949 U CN201620109949 U CN 201620109949U CN 205368399 U CN205368399 U CN 205368399U
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- bottleneck
- tissue culture
- culture flask
- rectangle
- cell
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Abstract
The utility model discloses a cell culture bottle relates to the bio -engineering technology field. This cell culture bottle includes body, bottleneck, rectangle lid and circular lid, and wherein the body lower part is the rectangle structure, and upper portion is the trapezium structure, the long limit and the rectangle structural connection of trapezium structure, and the minor face is connected with the bottleneck, it is uncovered that the one side of rectangle structure is equipped with the rectangle, and rectangle lid enclosing cover closes on the rectangle is uncovered, the tip of bottleneck is opened has the bottleneck, and the outside of bottleneck is equipped with the screw thread, and circular lid is twisted on the bottleneck through the screw thread soon, one deck collagen - silk fibroin complex film has been laid to the inner wall of body. The utility model discloses a lay collagen - silk fibroin complex film at the blake bottle inner wall, but promotes cell adheres and the adherence for cell fifferentiation hyperplasia improves cell output, for the blake bottle of biochemical peridium, have higher surface stability, and the transportation is the same with conventional consumptive material with the storage process, need not refrigerate or other special treatment, improved the cultivation that subtracts cell under serum or the serum -free condition.
Description
Technical field
This utility model relates to technical field of bioengineering, is specifically related to a kind of Tissue Culture Flask.
Background technology
In cell is cultivated, Tissue Culture Flask or culture dish are requisite utensils.Traditional Tissue Culture Flask many employings polystyrene material makes, and just can use after plasma surface treatment.This processing method is only applicable to the cell of routine and cultivates, and cultivates for subtracting the cell under serum or serum-free condition, then cannot meet the requirement of cell attachment.In view of the above problems, there is company to have developed biochemistry and be coated method, adopt albumen that culture bottle is coated, to promote absorption and the adherent growth of cell.But the culture bottle that this method processes generally requires cold preservation or other special handling, this is that the transport of culture bottle, storage etc. bring a lot of trouble.
Therefore, need badly a kind of easy to use, store simple, be suitable to the Tissue Culture Flask of cell attachment growth under subtracting serum or serum-free condition, to solve the problems referred to above.
Utility model content
The purpose of this utility model is in that the drawbacks described above overcoming prior art to exist, it is provided that one is suitable under subtracting serum or serum-free condition cell attachment growth, and store, the Tissue Culture Flask of convenient transportation.
This utility model is achieved through the following technical solutions:
A kind of Tissue Culture Flask, including body, bottleneck, rectangular cap and circular lid, the bottom of described body is rectangular configuration, and top is trapezium structure, and the long limit of described trapezium structure is connected with rectangular configuration, and minor face is connected with bottleneck；It is uncovered that the one side of described rectangular configuration is provided with rectangle, described rectangular cap enclosing cover be combined in rectangle uncovered on；The end of described bottleneck has bottleneck, and the outside of described bottleneck is provided with screw thread, and described circular lid passes through screwed on bottleneck；The inwall of described body is equipped with one layer of collagen-silk fibroin composite membrane.
As the preferred version of a kind of Tissue Culture Flask, described rectangular cap is connected on body by supersonic welding.
As the preferred version of a kind of Tissue Culture Flask, described rectangular cap is fastened on body by buckle structure.
As the preferred version of a kind of Tissue Culture Flask, the outside of described circular lid is evenly distributed with a plurality of anti-slip veins.
As the preferred version of a kind of Tissue Culture Flask, described circular lid is provided with passage and waterproof ventilated membrane.
As the preferred version of a kind of Tissue Culture Flask, described trapezium structure is provided with frosted and writes district, it is simple to record.
As the preferred version of a kind of Tissue Culture Flask, the side of described body is provided with containing mark, it is simple to observe culture fluid number.
As the preferred version of a kind of Tissue Culture Flask, described culture bottle adopts transparent polystyrene material to make.
As the preferred version of a kind of Tissue Culture Flask, described culture bottle is the sterile culture flask through gamma-ray irradiation or ultraviolet irradiation or soak with ethanol.
As the preferred version of a kind of Tissue Culture Flask, described culture bottle divides the specification having 5-1000ml by volume, and dividing by cell attachment area has 25～225cm2Specification.
The beneficial effects of the utility model are:
This utility model, by laying collagen-silk fibroin composite membrane at culture bottle inwall, can promote cell adhesion and adherent, accelerates cell differentiation propagation, improves cell yield；The operation that cell is cultivated, the processing and fabricating simplifying culture bottle is facilitated by the design of rectangular cap；The sealed environment of culture bottle is ensure that, it is therefore prevented that outside contamination by the design of circular lid.This Tissue Culture Flask, relative to biochemical coated culture bottle, has higher surface stability, and transport is identical with conventional consumptive material with the process of storage, does not need cold preservation or other special handling, substantially improves and subtract the cultivation of cell under serum or serum-free condition.
Accompanying drawing explanation
Explanation embodiment of the present utility model in order to become apparent or technical scheme of the prior art, simply introduce the accompanying drawing used required in embodiment or description of the prior art is done one below, drawings discussed below is embodiments more of the present utility model, for those of ordinary skill in the art, under the premise not paying creative work, it is also possible to obtain other accompanying drawing according to these accompanying drawings.
Fig. 1 is the overall structure schematic diagram of the Tissue Culture Flask that this utility model detailed description of the invention provides.
Fig. 2 is the mounting structure schematic diagram of the Tissue Culture Flask that this utility model detailed description of the invention provides.
In figure:
1, body；2, bottleneck；3, rectangular cap；4, circular lid；5, anti-slip veins.
Detailed description of the invention
Below in conjunction with drawings and Examples, this utility model is described in further detail.It is understood that specific embodiment described herein is used only for explaining this utility model, but not to restriction of the present utility model.It also should be noted that, for the ease of describing, accompanying drawing illustrate only the part relevant to this utility model but not entire infrastructure, and accompanying drawing is only for the purpose of description, and map not according to life size.
As shown in Figure 1 and Figure 2, present embodiments provide for a kind of Tissue Culture Flask, including body 1, bottleneck 2, rectangular cap 3 and circular lid 4, wherein body 1 can be divided into two parts, bottom rectangular shaped, the trapezoidal shape in top, and the long limit of trapezoidal shape is connected with rectangular shape, minor face is connected with bottleneck 2；It is uncovered that the one side of rectangle part is provided with rectangle, rectangular cap 3 enclosing cover be combined in rectangle uncovered on, here, the size that the size of rectangular cap 3 should be uncovered with rectangle is suitable, with ensure cover time culture bottle sealing.Further, the end of bottleneck 2 has common bottleneck, and the outside of bottleneck is provided with screw thread, and circular lid 4 passes through screwed on bottleneck；Further, on the inwall of body 1, uniform spreading is provided with one layer of collagen-silk fibroin composite membrane, specifically, this collagen-silk fibroin composite membrane is fixed on culture bottle inwall by viscose glue, such as light-sensitive emulsion etc., it addition, this collagen-silk fibroin composite membrane can also be attached on culture bottle inwall by the mode of physical absorption or covalent cross-linking.
This utility model, by arranging collagen-silk fibroin composite membrane at inwall, effectively improves Growth of Cells microenvironment, can make cell applied solid on culture bottle wall, and cell growth state is good, and cultivation effect is obvious, and cell yield is greatly improved；Relative to cellar culture bottle, its surface has higher stability, and transport and storage process do not need cold preservation or other special handling.
For convenience of the access that culture bottle inwall is laid collagen-silk fibroin composite membrane and cell, this utility model is provided with on body 1 that rectangle is uncovered and rectangular cap 3.As preferably, this rectangular cap 3 after cell is inoculated, can be connected on body 1 by supersonic welding or be fastened on body 1 by buckle structure.The mode of welding can provide good sealed environment for the growth of cell, it is ensured that the training quality of cell, also can avoid the pollution using other binding agent that culture fluid is caused simultaneously；And the mode of buckle is more flexible, it is possible to open and close rectangular cap 3 easily, be conducive to the cultivation situation of observational study cell at any time.It is preferred that, above-mentioned buckle structure includes the card convex being arranged in rectangular cap 3 and the draw-in groove being arranged on body 1, when card convex snaps in draw-in groove, it may be achieved its clamping function；Certainly, above-mentioned buckle structure can also be the card convex being arranged on body 1 and the draw-in groove being arranged in rectangular cap 3, can body 1 and rectangular cap 3 closely be engaged equally.
As preferably, in present embodiment, the outside of circular lid 4 is uniformly provided with a plurality of longitudinal anti-slip veins 5, in order to hold with a firm grip and rotary bottle cover；The end face of circular lid 4 is provided with passage and waterproof ventilated membrane, in order to culture bottle and the external world carry out gas exchange, and prevent extraneous dust, antibacterial etc. from entering in bottle.Further, the trapezoidal portions of this culture bottle body 1 is provided with frosted and writes district, can be used for recording the relevant information of cell；The side of body 1 is provided with containing mark, it is simple to the number of observation of cell solution or culture fluid；The bottom of culture bottle is provided with product batch number, it is simple to quality tracing.It addition, the bottleneck 2 of this culture bottle is inclined upwardly certain angle relative to body 1, this angle range preferably from 30-60 °；Being provided with of torticollis is beneficial to pipet or cell scrapes entrance body, is possible to prevent culture fluid to flow to bottle cap owing to rocking simultaneously.
Owing to often needing to observe the growth of bottle inner cell and adherent situation in cell cultivation process, this culture bottle should select the good plastic production of transparency, as: polystyrene；And, this culture bottle should carry out disinfection through gamma-ray irradiation or the method such as ultraviolet irradiation or soak with ethanol before use, and independent packaging seals in bag in medical tearing mode, to ensure the cleaning of culture bottle, aseptic.
This culture bottle divides by volume and is provided with the specification of 5-1000ml, conventional such as 125ml, 250ml, 500ml etc.；Divide by cell attachment area and be provided with 25～225cm2Specification, conventional such as 752、150㎝2、175㎝2Deng；The design of plurality of specifications meets the demand that different cell is cultivated well, improves cell training quality and efficiency, avoids the waste of culture fluid simultaneously.
This utility model preparation technology is simple, transport, convenient storage, it is to avoid cellar culture bottle needs the trouble of cold preservation and other process, is suitable for attached cell, especially subtracts the cultivation of attached cell under serum or serum-free condition.
Obviously, above-described embodiment of the present utility model is only used to clear this utility model example that illustrates, and is not the restriction to this utility model embodiment.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here without also cannot all of embodiment be given exhaustive.All any amendment, equivalent replacement and improvement etc. made within spirit of the present utility model and principle, should be included within this utility model scope of the claims.
Claims (10)
1. a Tissue Culture Flask, including body (1), bottleneck (2), rectangular cap (3) and circular lid (4), it is characterized in that, the bottom of described body (1) is rectangular configuration, top is trapezium structure, the long limit of described trapezium structure is connected with rectangular configuration, and minor face is connected with bottleneck (2)；It is uncovered that the one side of described rectangular configuration is provided with rectangle, described rectangular cap (3) enclosing cover be combined in rectangle uncovered on；The end of described bottleneck (2) has bottleneck, and the outside of described bottleneck is provided with screw thread, described circular lid (4) by screwed on bottleneck；The inwall of described body (1) is equipped with one layer of collagen-silk fibroin composite membrane.
2. Tissue Culture Flask according to claim 1, it is characterised in that described rectangular cap (3) is connected on body (1) by supersonic welding.
3. Tissue Culture Flask according to claim 1, it is characterised in that described rectangular cap (3) is fastened on body (1) by buckle structure.
4. Tissue Culture Flask according to claim 1, it is characterised in that the outside of described circular lid (4) is evenly distributed with a plurality of anti-slip veins (5).
5. the Tissue Culture Flask according to claim 1 or 4, it is characterised in that described circular lid (4) is provided with passage and waterproof ventilated membrane.
6. Tissue Culture Flask according to claim 1, it is characterised in that described trapezium structure is provided with frosted and writes district, it is simple to record.
7. Tissue Culture Flask according to claim 1, it is characterised in that the side of described body (1) is provided with containing mark, it is simple to observe culture fluid number.
8. Tissue Culture Flask according to claim 1, it is characterised in that described culture bottle adopts transparent polystyrene material to make.
9. the Tissue Culture Flask according to claim 1 or 8, it is characterised in that described culture bottle is the sterile culture flask through gamma-ray irradiation or ultraviolet irradiation or soak with ethanol.
10. Tissue Culture Flask according to claim 1, it is characterised in that described culture bottle divides the specification having 5-1000ml by volume, divides by cell attachment area and has 25～225cm2Specification.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620109949.XU CN205368399U (en) | 2016-02-03 | 2016-02-03 | Cell culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620109949.XU CN205368399U (en) | 2016-02-03 | 2016-02-03 | Cell culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205368399U true CN205368399U (en) | 2016-07-06 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620109949.XU Expired - Fee Related CN205368399U (en) | 2016-02-03 | 2016-02-03 | Cell culture bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205368399U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109706077A (en) * | 2019-01-21 | 2019-05-03 | 英诺维尔智能科技（苏州）有限公司 | A kind of Novel breathable culture bottle |
| CN111363681A (en) * | 2020-03-19 | 2020-07-03 | 江苏省人民医院（南京医科大学第一附属医院） | Integrated primary cell climbing culture bottle |
-
2016
- 2016-02-03 CN CN201620109949.XU patent/CN205368399U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109706077A (en) * | 2019-01-21 | 2019-05-03 | 英诺维尔智能科技（苏州）有限公司 | A kind of Novel breathable culture bottle |
| CN111363681A (en) * | 2020-03-19 | 2020-07-03 | 江苏省人民医院（南京医科大学第一附属医院） | Integrated primary cell climbing culture bottle |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160706 Termination date: 20210203 |
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| CF01 | Termination of patent right due to non-payment of annual fee |