CN103289897B - a cell culture flask - Google Patents

Abstract

The invention discloses a cell culture bottle, which belongs to the field of medical equipment. The cell culture bottle comprises a bottle body and a bottle cap, wherein the bottle cap is fixed at the bottle mouth of the bottle body, and an annular cell adherence medium layer is fixed inside the cavity of the bottle body, and is separated from the inner wall of the bottle body. The cell culture bottle has the following technical effects that as the cell adherence medium layer is additionally arranged in the cavity of the bottle body of the cell culture bottle, the superficial area for cell adherence culture inside the cell culture bottle is increased, and therefore, the density of the cell adhered to the inner wall of the cell culture bottle is increased and the once cell culture amount of the cell culture bottle is improved in a cell culture process.

Description

a cell culture flask

technical field

The invention relates to a cell culture bottle in the field of medical equipment.

Background technique

Cell culture flasks are important equipment for drug testing and implantable medical device testing. The sealing and safety of cell culture flasks are quite high, so they are widely favored by researchers in the pharmaceutical industry and medical device industry. Cell culture flasks can be divided into glass cell culture flasks and plastic cell culture flasks according to their materials. The shape of the bottom of the cell culture flasks can be round, square, rectangular, triangular, etc. The volume of the cell culture bottle has various specifications ranging from 500ml to 3L. The adherent area of the cell culture flask, that is, the surface area within the cell culture flask capable of carrying out cell adherent culture, includes various specifications ranging from 275cm ² to 3000cm ² .

However, there is a technical bottleneck in the current cell culture flasks, that is, the amount of cells cultured in a cell culture flask at one time cannot meet the requirements of the experiment, so more than one cell culture flask is needed for cell culture, resulting in cost in the research and development of medicines and medical devices. increase, waste of resources.

The reason for the above problems is that at present, the amount of cells cultured in a cell culture bottle is mainly increased by increasing the volume of the cell culture bottle. However, this approach cannot safely increase the amount of cells cultured in a cell culture bottle at one time, and the sealing performance of the cell culture bottle may be significantly reduced, increasing the risk of contamination during cell culture.

Contents of the invention

The purpose of the present invention is to overcome the deficiencies in the prior art and provide a cell culture bottle, which can increase the density of cells attached to the wall in the cell culture bottle during the cell culture process, thereby increasing the amount of cells cultured in the cell culture bottle at one time.

A technical solution to achieve the above object is: a cell culture bottle, including a bottle body and a bottle cap, and the bottle cap is fixed on the mouth of the bottle body;

In the inner cavity of the bottle body, an annular cell attachment medium layer is fixed, and the cell attachment medium layer is separated from the inner wall of the bottle body.

Further, the cell attachment medium layer is a non-woven folded layer.

Still further, a protrusion is provided at the center of the bottom of the inner cavity of the bottle body to radially fix the non-woven folded layer in the inner cavity of the bottle body.

Still further, the thickness of the non-woven fabric used in the wrinkled non-woven fabric layer is 2-3 mm, and it is woven from polyester fibers, and the diameter of the polyester fibers is 20-30 μm.

Still further, the inner wall of the bottle body and both sides of the non-woven folded layer are subjected to vacuum plasma surface treatment.

Further, the bottle cap is one of an airtight cap, a polyester cap, a breathable cap and a septum cap.

Further, the bottle body is made of glass.

Still further, the bottle body is made of borosilicate glass.

Further, the bottle body is made of plastic.

Still further, the bottle body is made of polystyrene or polyester.

A technical scheme of a cell culture bottle of the present invention is adopted, that is, in the inner chamber of the bottle body of the cell culture bottle, an annular cell attachment medium layer is fixed, and the cell attachment medium layer is in contact with the inner wall of the bottle body Separate technical solutions. Its technical effect is: because the cell adherence medium layer is increased in the inner cavity of the bottle body of the cell culture bottle, the surface area that can carry out cell adherence culture in the cell culture bottle is increased. The density of adherence in the culture bottle is increased, thereby increasing the amount of cells cultured in the cell culture bottle at one time.

Description of drawings

Fig. 1 is the front view of a kind of cell culture bottle of the present invention

Fig. 2 is a view along the line A-A of Fig. 1 .

Detailed ways

Referring to Fig. 1 and Fig. 2, in order to better understand the technical solution of the present invention, the inventors of the present invention will describe in detail below through specific embodiments in conjunction with the accompanying drawings:

Referring to Fig. 1, a cell culture bottle of the present invention includes a bottle body 1 and a bottle cap 2, and the bottle cap 2 is fixed on the bottle mouth 11 of the bottle body 1 to prevent the cells in the cell culture bottle from being polluted.

In the inner cavity 12 of the bottle body 1, an annular cell attachment medium layer 3 is fixed. inner wall separation.

The purpose of this design is: the inner wall and the outer wall of the cell adherent medium layer 3 can carry out cell adherent culture, like this, the surface area that can carry out cell adherent culture in the cell culture bottle increases to be original three times at least, during the cell culture process , the density of cells adhered to the wall in the cell culture flask is increased to three times the original, and the amount of cells cultured in the cell culture flask at one time can be increased to three times the original. Therefore, the amount of cells cultured in the cell culture flask at one time can meet the requirements of the experiment, and the cost and resource consumption in the research and development of medicine and medical devices are reduced.

In this embodiment, in the inner cavity 12 of the bottle body 1 of the cell culture bottle, the cell attachment medium layer 3 is a non-woven folded layer. The cell attachment medium layer 3 is made of non-woven fabric wrinkled layer to further increase the surface area in the cell culture flask for cell attachment culture. After adopting the non-woven folded layer, the surface area of the cell culture bottle that can be adhered to the cell culture is increased by at least five to seven times. The amount of cultured cells in a cell culture bottle can be increased by five to seven times. The amount of cells cultured in the cell culture bottle can further meet the requirements of the experiment, and the cost of medicine and medical device research and development is further reduced, and the loss of resources is reduced.

Another reason for adopting the folded layer of non-woven fabric is that: the permeability and air permeability of the non-woven fabric are all very good, which can ensure that the inner wall and the outer wall of the folded layer of the non-woven fabric and the inner wall of the bottle body 1 are pasted. The supply of nutrients and/or oxygen required for the cell growth of the wall can be well satisfied, and the survival rate of the cells in the culture process has been significantly improved.

In this embodiment, the wrinkled layer of non-woven fabric is preferably polyester fiber non-woven fabric, because its cell affinity is good, and the thickness of polyester fiber non-woven fabric is preferably 2-3 mm, such thickness makes the non-woven fabric The structural stability, mechanical properties, permeability and air permeability of the pleated layer can be effectively guaranteed. The polyester fiber non-woven fabric is woven from polyester fibers with a diameter of 20-30 μm. The purpose of selecting the polyester fiber is to effectively ensure the structural stability, mechanical properties, permeability and air permeability of the wrinkled layer of the non-woven fabric.

In order to ensure the structural stability of the non-woven folded layer, a protrusion 13 is provided at the center of the bottom of the inner cavity 12 of the bottle body 1 to radially fix the non-woven folded layer in the inner cavity 12 of the bottle body 1 .

The bottle caps 2 of cell culture bottles mainly include: airtight caps, polyester caps, vent caps, septum caps, etc. at present.

The bottom of the airtight cover does not have an inner pad, which is used for the airtight culture of cells and ensures the airtightness of the cell culture bottle during the cell culture process.

The polyester cap is often used for open cell culture. During the process of culturing cells with the cell culture bottle, the gas in the cell culture bottle and the environment can be separated by simply unscrewing the polyester cap. Gas is exchanged, and at the same time, the polyester cover is also easy to fix with the non-woven folded layer woven from polyester fibers.

A 0.2 μm hydrophobic filter membrane (not shown in the figure) is added to the bottom of the air-permeable cap to provide sterile gas exchange for cells cultured in the cell culture flask and reduce the risk of contamination of the cells in the cell culture flask. The cell culture bottle with the vented cap is often used for open culture of cells, especially suitable for cell culture in a CO ₂ incubator, especially for experiments that require long-term cell culture.

A septum (not shown) is added to the bottom of the septum cover, and the septum is fixed to the top of the non-woven pleated layer, and the septum has a pre-cut cut, which allows the nozzle, Or a pipette with a specification below 5ml pierces the septum to add liquid, absorb liquid or harvest cells to the cell culture bottle, reduce the chance of cells in the cell dispensing bottle being polluted, and maintain the closed and sterile cell culture bottle environment of.

From the point of view of cell adherent culture, the material of the bottle body 1 of the cell culture bottle can be glass or plastic. In this embodiment, the bottle body 1 of the cell culture bottle is preferably borosilicate glass, polystyrene or polyester. On the one hand, borosilicate glass, polyester or polystyrene has good affinity to cells, and on the other hand, it has good corrosion resistance, which is beneficial to the culture of cells in cell culture flasks, especially for long-term culture.

At the same time, the inner wall of the bottle body 1, and the inner wall and the outer wall of the non-woven folded layer can be treated by special surface modification to further improve the inner wall of the bottle body 1, and the inner wall and the outer wall of the non-woven folded layer. The cell affinity is convenient for the adherent culture of more cells. At the same time, after the cells propagate and adhere to the inner wall of the bottle body 1 and the two sides of the non-woven folded layer, they are relatively easy to digest. The special surface modification treatment includes vacuum plasma surface treatment and the like.

Claims (5)

1. a cell culture bottle, comprising bottle body (1) and bottle cap (2), described bottle cap (2) is fixed on the bottleneck (11) of described bottle body (1), is characterized in that: In the inner cavity (12) of the bottle body (1), an annular cell attachment medium layer (3) is fixed, and the cell attachment medium layer (3) is separated from the inner wall of the bottle body (1); The cell attachment medium layer (3) is a non-woven folded layer; The thickness of the non-woven fabric used in the folded layer of non-woven fabric is 2-3 mm, and it is woven from polyester fibers, and the diameter of the polyester fibers is 20-30 μm; The inner wall of the bottle body (1) and the two sides of the non-woven folded layer are all subjected to vacuum plasma surface treatment; The center of the bottom of the inner cavity (12) of the bottle body (1) is provided with a protrusion (13), and the non-woven fabric folded layer is radially fixed in the inner cavity (12) of the bottle body (1); The bottle cap (2) is a polyester cap; the bottle cap (2) is fixed to the folded layer of non-woven fabric. 2. A cell culture bottle according to claim 1, characterized in that: the bottle body (1) is made of glass. 3. A cell culture bottle according to claim 2, characterized in that: the bottle body (1) is made of borosilicate glass. 4. A cell culture bottle according to claim 1, characterized in that: the bottle body (1) is made of plastic. 5. A cell culture bottle according to claim 4, characterized in that: the bottle body (1) is made of polystyrene or polyester.