CN112522099A - Cell culture bottle - Google Patents
Cell culture bottle Download PDFInfo
- Publication number
- CN112522099A CN112522099A CN201910877163.0A CN201910877163A CN112522099A CN 112522099 A CN112522099 A CN 112522099A CN 201910877163 A CN201910877163 A CN 201910877163A CN 112522099 A CN112522099 A CN 112522099A
- Authority
- CN
- China
- Prior art keywords
- bottle
- feeding
- cell culture
- tube
- cap
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000004113 cell culture Methods 0.000 title claims abstract description 51
- 238000009423 ventilation Methods 0.000 claims abstract description 21
- 239000004816 latex Substances 0.000 claims description 10
- 229920000126 latex Polymers 0.000 claims description 10
- 239000004033 plastic Substances 0.000 claims description 10
- 229920003023 plastic Polymers 0.000 claims description 10
- 230000002572 peristaltic effect Effects 0.000 claims description 9
- 238000007789 sealing Methods 0.000 claims description 8
- 230000002209 hydrophobic effect Effects 0.000 claims description 5
- 230000005611 electricity Effects 0.000 claims description 4
- 239000012528 membrane Substances 0.000 claims description 3
- 230000004913 activation Effects 0.000 claims 1
- 239000003814 drug Substances 0.000 abstract description 12
- 229940079593 drug Drugs 0.000 abstract description 5
- 239000000463 material Substances 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 17
- 239000007789 gas Substances 0.000 description 10
- 238000000034 method Methods 0.000 description 9
- 235000015097 nutrients Nutrition 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- 238000012546 transfer Methods 0.000 description 7
- 238000012258 culturing Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- -1 polyethylene Polymers 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000000071 blow moulding Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
Landscapes
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a cell culture bottle, which comprises an upper bottle body, a lower bottle body, a bottle neck, a bottle cap, a feeding needle head and a feeding device, wherein the upper bottle body is in a trapezoidal structure, the lower bottle body is in a cuboid structure, the lower end face of the upper bottle body is connected with the lower bottle body, the upper end face of the upper bottle body is connected with the bottle neck, the bottle cap is connected onto the bottle neck, the bottle cap comprises an outer cap body and an inner cap body, inner threads are arranged on the inner side wall of the inner cap body, external threads matched with the internal threads are arranged on the outer side wall of the bottle neck, one end of the feeding needle head sequentially penetrates through the outer cap body and the inner cap. The cell culture bottle can quickly and conveniently feed materials or drugs, is convenient for ventilation, and obviously improves the cell culture quality.
Description
Technical Field
The invention relates to the technical field of cell culture experiment consumables, in particular to a cell culture bottle.
Background
In vitro culture of cells and tissues has become an essential element in life research and practice, and the types of cells cultured are numerous, ranging from viruses to bacteria and fungi, and from human cells to animal cells and plant cells. Some cells and tissues can grow in suspension, but a significant proportion of mammalian cells require surface adherence. The cell culture flask is used for cell culture, and can obtain a large amount of experimental data about basic growth information and culture process parameters of cells under the conditions of limited manpower, material resources and space, so the cell culture flask is widely applied to laboratory research and industrial production.
However, the existing cell culture flasks still have the following problems: (1) the method is characterized in that a large amount of oxygen is consumed in the process of culturing cells in a cell culture bottle, so that gas exchange between the gas in the bottle and the outside is needed, a bottle cap and a bottle body of the conventional cell culture bottle are loosely connected, and the gas in the bottle can be exchanged with the outside in the process of culturing the cells, but the method cannot realize full and rapid exchange between the cells in the bottle and the outside gas, and the speed of gas generation and consumption in the bottle becomes faster gradually along with rapid propagation of the cells, so that the method cannot meet the ventilation requirement in the process of cell growth and propagation, and further inhibits the cell growth; (2) the adherent cells need to be intervened by a plurality of medicines regularly in the process of culturing the cells in the culture bottle or nutrient substances are supplemented, the bottle cap needs to be unscrewed during the existing operation, a gun head, a liquid transfer gun and other sample adding devices need to be introduced, and the probability of cell pollution in the culture bottle is greatly increased.
Disclosure of Invention
In order to solve the technical problems in the background art, the invention aims to provide a cell culture bottle which can quickly and conveniently feed or add medicine, is convenient for ventilation and obviously improves the cell culture quality.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention provides a cell culture bottle, which comprises an upper bottle body, a lower bottle body, a bottle neck, a bottle cap, a feeding needle head and a feeding device, wherein the upper bottle body is in a trapezoidal structure, the lower bottle body is in a cuboid structure, the lower end surface of the upper bottle body is connected with the lower bottle body, the upper end surface of the upper bottle body is connected with the bottle neck, the bottle neck is connected with the bottle cap, the bottle cap comprises an outer cap body and an inner cap body, the inner side wall of the inner cap body is provided with internal threads, the outer side wall of the bottle neck is provided with external threads matched with the internal threads, the center of the inner side of the outer cap body is vertically provided with a rotating rod, the center of the upper portion of the inner cap body is provided with a through hole, one end of the rotating rod is fixedly connected with the inner side of the outer cap body, the other end, the arc scavenge port has been seted up on the outer lid, the arc bleeder vent has been seted up to the last corresponding department of inner cup, and adjustable arc bleeder vent exposes the part when rotating relatively between outer lid and the inner cup, is located dwang outside cover in the holding tank is equipped with ventilation fan, ventilation fan is connected with the power electricity, interior lid is close to bottleneck one side is equipped with hydrophobic ventilated membrane, reinforced syringe needle one end passes in proper order outer lid with interior lid stretch into to in the lower bottle, reinforced syringe needle other end pass through feeding catheter with feeding device is linked together.
The improved structure is characterized in that the outer cover body and the inner cover body are close to the rotating rod, an elastic latex annular hole is formed in the rotating rod, and the feeding needle head sequentially penetrates through the outer cover body and the elastic latex annular hole in the inner cover body to stretch into the lower bottle body. Through setting up latex elasticity annular hole, be convenient for reinforced syringe needle pass outer lid and inner cup body and get into to bottle down in, also have good sealed effect simultaneously, guaranteed sterile environment in the cell culture bottle.
The improved structure is characterized in that a multi-way pipeline is arranged between the feeding guide pipe and the feeding device, the multi-way pipeline comprises a main pipeline and at least one feeding branch pipeline arranged on one side of the main pipeline, one end of the main pipeline is communicated with the feeding guide pipe, the other end of the main pipeline is arranged in a sealing mode, one end of the feeding branch pipeline is communicated with the feeding device through a feeding branch pipe, and a plastic buckle is arranged at the joint of the feeding branch pipeline and the feeding branch pipe. Through designing the quantity of reinforced branch pipe way in to the multichannel pipeline to realize tee bend, cross, five-way etc. conveniently to injecting into the medicine or the nutrient substance of multiple different grade type, mutual noninterference in the cell culture bottle, and can effectually avoid the cell in the cell culture bottle to appear polluting.
The further improvement is that the feeding device comprises a feeding bottle and a peristaltic pump, the peristaltic pump is installed on the feeding branch pipe, and the free end of the feeding branch pipe extends into the feeding bottle. When the feeding bottle is used, if a large amount of nutrient substances are required to be added into the cell culture bottle, the nutrient substances in the feeding bottle can be automatically conveyed into the cell culture bottle through the switching or the operation of the peristaltic pump.
The improved feeding device comprises a tube body, a piston and a piston rod, wherein one end of the tube body is communicated with the feeding branch tube, the piston is arranged inside the tube body, the piston is in sealing contact with the inner wall of the tube body and can slide up and down, one end of the piston rod is fixedly connected with the piston, and the other end of the piston rod penetrates through the rear end of the tube body and is provided with a handheld part. When the device is used, if a small amount of medicines with different types and complementary interference are required to be injected into the cell culture bottle, the medicines in the tube body can be added into the cell culture bottle by pressing the piston rod downwards.
The improvement is that the connecting part of the pipe body and the feeding branch pipe is provided with a plastic buckle, and the surface of the pipe body is provided with scale marks. The sealing of the connection part of the tube body and the feeding branch tube is ensured by arranging the plastic buckle, and the quantity of the medicine added into the cell culture bottle is strictly controlled by arranging the scale marks on the surface of the tube body.
In a further improvement, the power supply is arranged inside the outer cover body and is electrically connected with the starting switch positioned outside the outer cover body.
Compared with the prior art, the invention has the following beneficial effects:
(1) the bottle cap of the cell culture bottle comprises an outer cap body and an inner cap body, wherein the outer cap body and the inner cap body are rotatably connected through a rotating rod, and arc-shaped ventilation holes formed in corresponding positions on the outer cap body are matched with arc-shaped ventilation holes formed in corresponding positions on the inner cap body through arc-shaped ventilation holes in the outer cap body, so that the exposed parts of the arc-shaped ventilation holes can be flexibly regulated and controlled, the gas exchange speed between the cell culture bottle and the outside can be further regulated, the gas requirements of cells in different production stages can be met, a ventilation fan is arranged between the outer cap body and the inner cap body, gas accumulated in the cell culture bottle is actively and quickly discharged and ventilated, and a hydrophobic and breathable film is arranged on one side, close to a bottle;
(2) the feeding needle head conveniently and rapidly penetrates through the latex elastic annular holes in the outer cover body and the inner cover body to enter the lower bottle body, a good sealing effect is achieved, the sterile environment in the cell culture bottle is guaranteed, the other end of the feeding needle head is communicated with the multi-way pipeline through the feeding guide pipe, the number of the feeding branch pipelines in the multi-way pipeline is designed to achieve three-way, four-way and five-way functions and the like, various medicines or nutrient substances which are different in types and do not interfere with each other can be conveniently injected into the cell culture bottle through the feeding device, and cells in the cell culture bottle can be effectively prevented from being polluted.
Drawings
The invention is described in further detail below with reference to specific embodiments and with reference to the following drawings.
FIG. 1 is a schematic view showing the structure of a cell culture flask and a feeder in accordance with example 1;
fig. 2 is a schematic structural view of the bottle cap according to embodiment 1;
fig. 3 is a schematic view of the inner structure of the bottle cap according to embodiment 1;
FIG. 4 is a schematic structural view of an upper lid body in embodiment 1;
FIG. 5 is a schematic structural view of a lower cover in embodiment 1;
FIG. 6 is a schematic view showing the structure of the cell culture flask and the feeder device in accordance with example 2;
wherein, the specific reference numbers are: go up bottle 1, lower bottle 2, bottleneck 3, bottle lid 4, dwang 5, arc scavenge port 6, starting switch 7, interior lid 8, arc bleeder vent 9, through-hole 10, latex elasticity annular hole 11, hydrophobic ventilated membrane 12, ventilation fan 13, power 14, reinforced syringe needle 15, feeding pipe 16, enclosing cover 17, trunk line 18, reinforced small transfer line 19, plastics buckle 20, feeding branch pipe 21, peristaltic pump 22, reinforced bottle 23, body 24, piston 25, piston rod 26, handheld portion 27, scale sign 28.
Detailed Description
Example 1
Embodiment 1 of the present invention discloses a cell culture bottle, as shown in fig. 1, comprising an upper bottle body 1, a lower bottle body 2, a bottle neck 3, a bottle cap 4, a feeding needle 15 and a feeding device, wherein the upper bottle body 1 is in a trapezoidal structure, the lower bottle body 2 is in a rectangular structure, the lower end surface of the upper bottle body 1 is connected with the lower bottle body 2, the upper end surface of the upper bottle body 1 is connected with the bottle neck 3, the bottle cap 4 is connected with the bottle neck 3, as shown in fig. 2 to 5, the bottle cap 4 comprises an outer cap body 17 and an inner cap body 8, the inner side wall of the inner cap body 8 is provided with an internal thread, the outer side wall of the bottle neck 3 is provided with an external thread matching with the internal thread, the center of the inner side of the outer cap body 17 is vertically provided with a rotating rod 5, the center of the inner cap body 8 is provided with a through hole 10, the holding tank has been formed between interior lid 8 and the enclosing cover body 17, arc scavenge port 6 has been seted up on the enclosing cover body 17, arc bleeder vent 9 has been seted up to corresponding department on the interior lid 8, adjustable arc bleeder vent 9 exposes the part when rotating relatively between enclosing cover body 17 and the interior lid 8, and then can adjust the speed that cell culture bottle and external gas exchange carried out, thereby satisfy different production phase cells to gaseous demand, the dwang 5 outside cover that is located in the holding tank is equipped with ventilation fan 13, ventilation fan 13 is connected with power 14 electricity, power 14 installs at the enclosing cover body 17 inboardly, and power 14 is connected with starting switch 7 that is located the enclosing cover body 17 outside electricity, the gaseous quick discharge of accumulation in the cell culture bottle is initiatively discharged through installation ventilation fan 13, and take a breath.
One end of the feeding needle 15 sequentially penetrates through the outer cover body 17 and the inner cover body 8 to extend into the lower bottle body 2, and the other end of the feeding needle 15 is communicated with a feeding device through a feeding conduit 16. Wherein, be close to on enclosing cover body 17 and the interior lid 8 and locate to be provided with latex elasticity annular hole 11 in dwang 5, 15 heads of reinforced needle pass outer lid 17 in proper order with the interior latex elasticity annular hole 11 on the lid 8 stretch into to bottle 2 down in, through setting up latex elasticity annular hole 11, be convenient for reinforced syringe needle 15 pass outer lid 17 and interior lid 8 enter into to bottle 2 down in, also have good sealed effect simultaneously, guaranteed aseptic environment in the cell culture bottle. Be provided with the multi-pass pipe between feeding pipe 16 and the feeding device, the multi-pass pipe includes trunk line 18 and at least one sets up in the reinforced small transfer line 19 of trunk line 18 one side, trunk line 18 one end is linked together with feeding pipe 16, the sealed setting of trunk line 18 other end, 19 one end of reinforced small transfer line is linked together with the feeding device through feeding branch pipe 21, reinforced small transfer line 19 is provided with plastics buckle 20 with feeding branch pipe 21 junction, plastics buckle 20 can guarantee the sealed of reinforced small transfer line 19 with feeding branch pipe 21 junction, design through the quantity to reinforced small transfer line 19 in the multi-pass pipe, with realize the tee bend, the cross, five-way etc., conveniently to injecting multiple different grade type in the cell culture bottle, mutually noninterfere's medicine or nutrient substance, and can effectually avoid the interior cell of cell culture bottle to appear polluting. The feeding device comprises a feeding bottle 23 and a peristaltic pump 22, the peristaltic pump 22 is installed on the feeding branch pipe 21, and the free end of the feeding branch pipe 21 extends into the feeding bottle 23. When in use, if a large amount of nutrient substances are required to be added into the cell culture bottle, the nutrient substances in the feeding bottle 23 can be automatically conveyed into the cell culture bottle by switching on or off or operating the peristaltic pump 22.
The upper bottle body 1 and the lower bottle body 2 are made of polyethylene or polypropylene, can be formed by blow molding through injection molding, and can be repeatedly used.
The ventilation fan 13 may be a conventional micro ventilation fan, the minimum size of the ventilation fan 13 may be only 8 mm long and wide, 4 mm thick, similar to a shirt button, and may have a lifetime of 50000 hours, and the power source 14 may be driven by a button battery.
One side of the inner cover body 8 close to the bottleneck 3 is provided with a hydrophobic breathable film 12, which is beneficial to ventilation and ensures the gas exchange process to be sterile. The outer side walls of the outer cover body 17 and the inner cover body 8 are provided with indicating marks, the indicating marks on the first cover body are numbers, the indicating marks on the second cover body are reference lines, and the sizes of the arc-shaped ventilation ports 6 on the bottle caps 4 are judged and regulated through the numbers opposite to the reference lines.
Example 2
The embodiment 2 of the invention discloses a cell culture bottle, the embodiment 2 has the same basic characteristics as the embodiment 1, and the two embodiments have the following distinguishing technical characteristics: as shown in fig. 6, feeding device includes body 24, piston 25 and piston rod 26, body 24 one end is linked together with feeding branch 21, body 24 is inside to be provided with piston 25, piston 25 and 24 inner walls of body are sealing contact and can slide from top to bottom, piston rod 26 one end and piston 25 fixed connection, the piston rod 26 other end runs through body 24 rear end and installs handheld portion 27, in the time of the use, if need to inject multiple different grade type into the cell culture bottle, during the comparatively small amount of medicine of complementary interference, the accessible pushes down piston rod 26, add the medicine in the body 24 to the cell culture bottle. Wherein, the junction of body 24 and feeding branch 21 is provided with plastics buckle 20, and body 24 is equipped with scale mark 28 on the surface. The sealing of the connection part of the tube body 24 and the feeding branch tube 21 is ensured by arranging the plastic buckle 20, and the quantity of the medicine added into the cell culture bottle is strictly controlled by arranging the scale marks 28 on the surface of the tube body 24.
The present invention has been described in terms of specific examples, which are provided to aid understanding of the invention and are not intended to be limiting. For a person skilled in the art to which the invention pertains, several simple deductions, modifications or substitutions may be made according to the idea of the invention.
Claims (7)
1. A cell culture bottle is characterized by comprising an upper bottle body, a lower bottle body, a bottleneck, a bottle cap, a feeding needle head and a feeding device, wherein the upper bottle body is in a trapezoidal structure, the lower bottle body is in a cuboid structure, the lower end face of the upper bottle body is connected with the lower bottle body, the upper end face of the upper bottle body is connected with the bottleneck, the bottle cap comprises an outer cap body and an inner cap body, the inner side wall of the inner cap body is provided with an internal thread, the outer side wall of the bottleneck is provided with an external thread matched with the internal thread, the center of the inner side of the outer cap body is vertically provided with a rotating rod, the upper center of the inner cap body is provided with a through hole, one end of the rotating rod is fixedly connected with the inner side of the outer cap body, the other end of the rotating rod penetrates through the through, the arc scavenge port has been seted up on the outer lid, the arc bleeder vent has been seted up to the last corresponding department of inner cup, and adjustable arc bleeder vent exposes the part when rotating relatively between outer lid and the inner cup, is located dwang outside cover in the holding tank is equipped with ventilation fan, ventilation fan is connected with the power electricity, interior lid is close to bottleneck one side is equipped with hydrophobic ventilated membrane, reinforced syringe needle one end passes in proper order outer lid with interior lid stretch into to in the lower bottle, reinforced syringe needle other end pass through feeding catheter with feeding device is linked together.
2. The cell culture bottle of claim 1, wherein the outer cover and the inner cover are provided with elastic latex ring holes near the rotating rod, and the feeding needle sequentially passes through the elastic latex ring holes on the outer cover and the inner cover and extends into the lower bottle.
3. The cell culture bottle according to claim 2, wherein a multi-way pipeline is arranged between the feeding conduit and the feeding device, the multi-way pipeline comprises a main pipeline and at least one feeding branch pipeline arranged on one side of the main pipeline, one end of the main pipeline is communicated with the feeding conduit, the other end of the main pipeline is arranged in a sealing manner, one end of the feeding branch pipeline is communicated with the feeding device through a feeding branch pipe, and a plastic buckle is arranged at the joint of the feeding branch pipeline and the feeding branch pipe.
4. The cell culture bottle of claim 3, wherein the feeding device comprises a feeding bottle and a peristaltic pump, the peristaltic pump is mounted on the feeding branch pipe, and the free end of the feeding branch pipe extends into the feeding bottle.
5. The cell culture bottle according to claim 3, wherein the feeding device comprises a tube, a piston and a piston rod, one end of the tube is communicated with the feeding branch tube, the piston is arranged in the tube and is in sealing contact with the inner wall of the tube and can slide up and down, one end of the piston rod is fixedly connected with the piston, and the other end of the piston rod penetrates through the rear end of the tube and is provided with a handheld part.
6. The cell culture bottle of claim 5, wherein a plastic buckle is arranged at the connection part of the tube body and the feeding branch tube, and scale marks are arranged on the surface of the tube body.
7. The cell culture flask according to claim 1, wherein the power supply is mounted inside the outer lid, and the power supply is electrically connected to an activation switch located outside the outer lid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910877163.0A CN112522099A (en) | 2019-09-17 | 2019-09-17 | Cell culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910877163.0A CN112522099A (en) | 2019-09-17 | 2019-09-17 | Cell culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112522099A true CN112522099A (en) | 2021-03-19 |
Family
ID=74974724
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910877163.0A Pending CN112522099A (en) | 2019-09-17 | 2019-09-17 | Cell culture bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112522099A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2757148A1 (en) * | 2013-01-18 | 2014-07-23 | Eppendorf Ag | Culture bottle |
| CN207276632U (en) * | 2017-09-14 | 2018-04-27 | 北京旌准医疗科技有限公司 | Disposable Tissue Culture Flask |
| CN207418765U (en) * | 2017-11-08 | 2018-05-29 | 齐齐哈尔医学院 | Portable suspension cell culture and chemicals dosing plant |
| CN207672071U (en) * | 2017-11-08 | 2018-07-31 | 齐齐哈尔医学院 | Convenient for the attached cell culture bottle of dosing |
| CN207699603U (en) * | 2017-11-08 | 2018-08-07 | 齐齐哈尔医学院 | A kind of suspension cell synchronizes the device of inoculation and dosing |
| CN207918869U (en) * | 2017-12-29 | 2018-09-28 | 宁夏医科大学总医院 | Tissue Culture Flask |
-
2019
- 2019-09-17 CN CN201910877163.0A patent/CN112522099A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2757148A1 (en) * | 2013-01-18 | 2014-07-23 | Eppendorf Ag | Culture bottle |
| CN207276632U (en) * | 2017-09-14 | 2018-04-27 | 北京旌准医疗科技有限公司 | Disposable Tissue Culture Flask |
| CN207418765U (en) * | 2017-11-08 | 2018-05-29 | 齐齐哈尔医学院 | Portable suspension cell culture and chemicals dosing plant |
| CN207672071U (en) * | 2017-11-08 | 2018-07-31 | 齐齐哈尔医学院 | Convenient for the attached cell culture bottle of dosing |
| CN207699603U (en) * | 2017-11-08 | 2018-08-07 | 齐齐哈尔医学院 | A kind of suspension cell synchronizes the device of inoculation and dosing |
| CN207918869U (en) * | 2017-12-29 | 2018-09-28 | 宁夏医科大学总医院 | Tissue Culture Flask |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP2623587B1 (en) | Apparatus and method for automated replacement of culture medium and performing toxicity assays on live cells | |
| CN112522099A (en) | Cell culture bottle | |
| CN210916096U (en) | Liquid supplementing device for cell culture bag | |
| CN207608562U (en) | The attached cell of novel loading combines culture apparatus | |
| CN208964946U (en) | A kind of storage culture medium prevents the culture bottle of germ contamination | |
| CN203999609U (en) | Anerobe dialysis culturing device | |
| CN204198747U (en) | A kind of cell culture bags of convenient operation | |
| CN205170845U (en) | Cultivate device of alga filamentous | |
| CN211771277U (en) | Microorganism isolated culture device | |
| CN210065722U (en) | Biological culture experimental device | |
| RU45733U1 (en) | INSTALLATION FOR IMPLEMENTATION OF MICROBIOLOGICAL PROCESSES | |
| CN212077079U (en) | Cell culture bottle | |
| CN208964921U (en) | A kind of novel culture bottle storing culture medium | |
| CN106281989A (en) | Laboratory combination type tray solid-state fermentation installation | |
| CN207330807U (en) | A kind of quick preparation device of monoclonal antibody | |
| CN205907269U (en) | Hair form root liquid culture plate | |
| CN219297544U (en) | Partitioned culture device for mouse cells | |
| CN205329045U (en) | Two spore mushroom matrix fermenting installation | |
| CN204325353U (en) | A kind of screening installation of organic exhaust gas degradation bacteria | |
| CN104263637B (en) | A kind of screening installation of organic exhaust gas degradation bacteria | |
| CN211246258U (en) | Biological material mixing apparatus for bioengineering | |
| CN220788524U (en) | Sterile detection bottle for liquid culture | |
| CN217868918U (en) | Cell culture dish | |
| CN203187679U (en) | Biological fermentation culture bottle | |
| CN211394502U (en) | Culture apparatus for cell experiments |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |