CN207918869U

CN207918869U - Tissue Culture Flask

Info

Publication number: CN207918869U
Application number: CN201721907166.7U
Authority: CN
Inventors: 卢震辉; 周瑜
Original assignee: General Hospital of Ningxia Medical University
Current assignee: General Hospital of Ningxia Medical University
Priority date: 2017-12-29
Filing date: 2017-12-29
Publication date: 2018-09-28
Anticipated expiration: 2027-12-29

Abstract

A kind of Tissue Culture Flask includes cell culture bottle,Cell culture lid,The cell culture bottle is tightly connected with cell culture lid,Cell culture lid includes the first lid,Second lid,Third lid,The lower surface of first lid is equipped with shaft,The center of second lid is equipped with through-hole,Second lid is set in the shaft of the first lid,So that the first lid and the second lid are rotatablely connected,The first arcuate socket is additionally provided on first lid,Accordingly,Second lid is equipped with the second arcuate socket,First arcuate socket and the second arcuate socket match,To generate ventilation mouth when rotating the first lid or the second lid,To adjust Tissue Culture Flask and extraneous air charge rate,The inner wall of second lid is equipped with internal thread,The outer wall of corresponding third lid is equipped with external screw thread,So that the second lid and third lid are threadedly coupled,The upper end of third lid is equipped with cell filtering net,Pollution is generated in Tissue Culture Flask to prevent fungi from entering,The inner wall of third lid is equipped with internal thread.

Description

Tissue Culture Flask

Technical field

The utility model is related to bioengineering vessel technical field more particularly to a kind of Tissue Culture Flasks.

Background technology

Tissue Culture Flask is in cell culture.Either zooblast, plant cell or mushroom are in incubation In can all absorb gas and generate new gas where to change gaseous environment in Tissue Culture Flask or change cell Liquid environment, for example, liquid pH value.Therefore when carrying out cell culture, Tissue Culture Flask can not be made to be fully sealed.Usually The bottle cap of Tissue Culture Flask is threadedly coupled with body, and when carrying out cell culture, bottle cap is connect with body more to be loosened, so that bottle It is interior to carry out gas exchanges with extraneous.But this mode can not accurately allow gas and ambient atmos complete exchange in bottle. Meanwhile during cell proliferation, with increasing for cell, gas generates or the speed of consumption is also becoming faster.Aforesaid way is past Toward the ventilation needs that cannot be satisfied cell.And when bottle cap is poorly sealed with body, the fungi in air is easily accessible cell training Bottle is supported, to generate pollution to the culture environment in Tissue Culture Flask.

Invention content

In view of this, it is necessary to provide a kind of Tissue Culture Flasks simple in structure, convenient for ventilation.

A kind of Tissue Culture Flask includes cell culture bottle, cell culture lid, and the cell culture bottle is trained with cell It supports lid to be tightly connected, cell culture lid includes the first lid, the second lid, third lid, the following table of first lid Face is equipped with shaft, and the center of the second lid is equipped with through-hole, and the second lid is set in the shaft of the first lid, so that the first lid It is rotatablely connected with the second lid, the first arcuate socket is additionally provided on the first lid, correspondingly, the second lid is equipped with the second arc Hole, the first arcuate socket and the second arcuate socket match, to generate ventilation mouth when rotating the first lid or the second lid, to adjust Ganglion cell's culture bottle and extraneous air charge rate, the inner wall of the second lid are equipped with internal thread, and the outer wall of corresponding third lid is set There is external screw thread, so that the second lid and third lid are threadedly coupled, the upper end of third lid is equipped with cell filtering net, true to prevent Bacterium, which enters, generates pollution in Tissue Culture Flask, the inner wall of third lid is equipped with internal thread, so that third lid and Tissue Culture Flask Body is threadedly coupled.

Preferably, the cell culture lid further includes air interchanger, to be carried out actively to the gas in Tissue Culture Flask Ventilation, the air interchanger includes mini-fan, battery, start stop switch, and the mini-fan is connect with battery with conducting wire, start and stop It switchs on the conducting wire being arranged between mini-fan and battery, is taken a breath to Tissue Culture Flask with controlling mini-fan, first The lower end of lid is equipped with groove, and mini-fan is arranged in the groove of the first lid, and battery is fixedly connected with the first lid, start and stop Switch is arranged on the upper surface of the first lid, to facilitate personnel to operate.

Preferably, the outer wall of first lid and the second lid is equipped with anti-skid chequer, to facilitate personnel to operate.

Preferably, the outer wall of the first lid and the second lid is equipped with cue mark, to facilitate personal observations' cell culture lid The size of the ventilation mouth of body.

Advantageous effect：The cell culture lid of the Tissue Culture Flask of the utility model includes the first lid, the second lid, the Three lids, the second arcuate socket on the first arcuate socket and the second lid on the first lid match, and can adjust cell culture Bottle and extraneous air exchanging rate, third lid are equipped with cell filtering net, can prevent fungi from entering Tissue Culture Flask, cause thin Born of the same parents' culture bottle pollutes.

Description of the drawings

Fig. 1 is the structural schematic diagram of the Tissue Culture Flask of the utility model.

Fig. 2 is the assembling structure schematic diagram of the cell culture lid of the Tissue Culture Flask of the utility model.

Fig. 3 is the fractionation structural representation of the cell culture lid of the Tissue Culture Flask of the utility model.

In figure：Tissue Culture Flask 10, cell culture bottle 20, cell culture lid 30, the first lid 301, shaft 3011, First arcuate socket 3012, the second lid 302, the second arcuate socket 3021, third lid 303, cell filtering net 3031, air interchanger 40, mini-fan 401, start stop switch 402, cue mark 50.

Specific implementation mode

It, below will be to required use in embodiment in order to illustrate more clearly of the technical solution of the utility model embodiment Attached drawing be briefly described, it should be apparent that, the accompanying drawings in the following description is some embodiments of the utility model, for this For the those of ordinary skill of field, without creative efforts, others are can also be obtained according to these attached drawings Attached drawing.

Fig. 1, Fig. 2 and Fig. 3 are please referred to, Tissue Culture Flask 10 includes cell culture bottle 20, cell culture lid 30, described Cell culture bottle 20 is tightly connected with cell culture lid 30, and cell culture lid 30 includes the first lid 301, the second lid 302, the lower surface of third lid 303, first lid 301 is equipped with shaft 3011, and the center of the second lid 302, which is equipped with, to be led to Hole, the second lid 302 are set in the shaft 3011 of the first lid 301, so that the first lid 301 and the second lid 302 rotate It connects, the first arcuate socket 3012 is additionally provided on the first lid 301, correspondingly, the second lid 302 is equipped with the second arcuate socket 3021, the first arcuate socket 3012 and the second arcuate socket 3021 match, with when rotating the first lid 301 or the second lid 302 Ventilation mouth is generated, to adjust Tissue Culture Flask 10 and extraneous air charge rate, the inner wall of the second lid 302 is equipped with internal thread, The outer wall of corresponding third lid 303 is equipped with external screw thread, so that the second lid 302 and third lid 303 are threadedly coupled, third lid The upper end of body 303 is equipped with cell filtering net 3031, and pollution, third lid are generated in Tissue Culture Flask 10 to prevent fungi from entering 303 inner wall is equipped with internal thread, so that third lid 303 is threadedly coupled with cell culture bottle 20.

In practical cell cultivation process, increase with the quantity of cell, the rate of cell ventilation is also being accelerated, this practicality Novel Tissue Culture Flask 10 can accurately be adjusted in incubation and be changed by rotating the first lid 301 and the second lid 302 The speed of gas, to meet the growth needs of cell.Simultaneously certain cells with external environment variation, for the demand of gas Also can be different, such as plant cell carries out photosynthesis daytime, when illumination is stronger, the carbon dioxide needed is more, in illumination When weaker, the carbon dioxide needed is less.At night, plant cell will be breathed, and the oxygen needed is more.The utility model The size of ventilation mouth can be adjusted flexibly, by the first bottle cap of simple rotation or the second bottle cap to meet different periods plant Demand of the cell for gas.The third lid 303 of Tissue Culture Flask 10 is equipped with cell filtering net 3031, can prevent in air Fungi enter Tissue Culture Flask 10, cause Tissue Culture Flask 10 pollute.

Further, the cell culture lid 30 further includes air interchanger 40, with to the gas in Tissue Culture Flask 10 Carry out active ventilation, the air interchanger 40 include mini-fan 401, battery, start stop switch 402, the mini-fan 401 with Battery is connected with conducting wire, and start stop switch 402 is arranged on the conducting wire between mini-fan 401 and battery, to control mini-fan 401 pairs of Tissue Culture Flasks 10 are taken a breath, and the lower end of the first lid 301 is equipped with groove, and mini-fan 401 is arranged in the first lid In 301 groove, battery is fixedly connected with the first lid 301, and start stop switch 402 is arranged on the upper surface of the first lid 301, To facilitate personnel to operate.

The gas that accumulated in bottle quickly can be discharged for the air interchanger 40 of cell culture lid 30, to reduce cell training Support gas harmful effect caused by cell in bottle 10.

Further, the outer wall of first lid, 301 and second lid 302 is equipped with anti-skid chequer, to facilitate personnel to operate.

Further, the outer wall of the first lid 301 and the second lid 302 is equipped with cue mark 50, to facilitate personal observations The size of the ventilation mouth of cell culture lid 30.

In a better embodiment, the cue mark 50 of the first lid 301 is number, the indicateing arm of the second lid 302 Line on the basis of note 50, with the size for mouth of being taken a breath by the digital judgement of datum line face.

Above disclosures are merely preferred embodiments of the utility model, with this utility model cannot be limited certainly Interest field, those skilled in the art can understand all or part of the processes for realizing the above embodiment, and according to this reality The equivalent variations made by novel claim, still fall within the scope of the utility model.

Claims

1. a kind of Tissue Culture Flask, it is characterised in that：Including cell culture bottle, cell culture lid, the Tissue Culture Flask Body and cell culture lid are tightly connected, and cell culture lid includes the first lid, the second lid, third lid, and described first The lower surface of lid is equipped with shaft, and the center of the second lid is equipped with through-hole, and the second lid is set in the shaft of the first lid, with So that the first lid and the second lid is rotatablely connected, the first arcuate socket is additionally provided on the first lid, correspondingly, the second lid is equipped with Second arcuate socket, the first arcuate socket and the second arcuate socket match, to generate ventilation when rotating the first lid or the second lid Mouthful, to adjust Tissue Culture Flask and extraneous air charge rate, the inner wall of the second lid is equipped with internal thread, corresponding third lid Outer wall be equipped with external screw thread so that the second lid and third lid are threadedly coupled, the upper end of third lid is equipped with cell filtering net, Pollution is generated in Tissue Culture Flask to prevent fungi from entering, the inner wall of third lid is equipped with internal thread so that third lid with it is thin Born of the same parents cultivate bottle and are threadedly coupled.

2. Tissue Culture Flask as described in claim 1, it is characterised in that：The cell culture lid further includes air interchanger, To carry out active ventilation to the gas in Tissue Culture Flask, the air interchanger includes mini-fan, battery, start stop switch, institute Mini-fan is stated to be connect with battery with conducting wire, it is micro- to control on the conducting wire that start stop switch is arranged between mini-fan and battery Type fan takes a breath to Tissue Culture Flask, and the lower end of the first lid is equipped with groove, and mini-fan is arranged in the recessed of the first lid In slot, battery is fixedly connected with the first lid, and start stop switch is arranged on the upper surface of the first lid, to facilitate personnel to operate.

3. Tissue Culture Flask as described in claim 1, it is characterised in that：The outer wall of first lid and the second lid is equipped with Anti-skid chequer, to facilitate personnel to operate.

4. Tissue Culture Flask as described in claim 1, it is characterised in that：The outer wall of first lid and the second lid is equipped with instruction Label, to facilitate the size of the ventilation mouth of personal observations' cell culture lid.