CN211394501U

CN211394501U - Suspension cell culture bottle

Info

Publication number: CN211394501U
Application number: CN201921999129.2U
Authority: CN
Inventors: 戴以武; 缪金伟
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-11-19
Filing date: 2019-11-19
Publication date: 2020-09-01
Anticipated expiration: 2029-11-19

Abstract

The utility model discloses a suspension cell culture bottle, which comprises an air inlet hole, a hydrophobic breathable film, a sample inlet, a sampling port, a first ring and a second ring, wherein the air inlet hole is used for further facilitating air to enter the bottle body, thereby further ensuring oxygen and carbon dioxide required by cell growth, the hydrophobic breathable film is used for facilitating air to enter the bottle body through the hole arranged at the top of the bottle cap and the air inlet hole, simultaneously avoiding the leakage of liquid in the bottle body, when the bottle body needs to be shaken, an operator firstly extends a thumb into the first ring and further inserts a forefinger and a second finger into the two second rings respectively, thereby realizing the fixation of the positions of the first ring and the second ring on the fingers of the operator, improving the stability when the operator holds the bottle body, avoiding the situation that the operator slides down when holding the bottle body, the practicability of the equipment is improved.

Description

Suspension cell culture bottle

Technical Field

The utility model relates to a culture bottle specifically is a suspension cell culture bottle.

Background

Suspension culture refers to a tissue culture system for culturing single cells and small cell clusters in a liquid culture medium which is constantly stirred or shaken, and is a culture mode of anchorage-independent cells. Certain anchorage-dependent cells can also be cultured using this method, after adaptation and selection. It is relatively simple to increase the scale of suspension culture, as long as the volume is increased. Beyond 5mm in depth, the medium needs to be agitated to ensure adequate gas exchange. A culture method in which cells are always in a dispersed suspension in a culture solution by shaking or rotating a device;

the abundant entering of outside air can't be guaranteed to current suspension cell culture bottle to can't guarantee required oxygen of cell growth and carbon dioxide, current suspension cell culture bottle is simultaneously because the surface is smooth, and stability when operating personnel handheld rock is relatively poor, the condition of landing appears easily, and current suspension cell culture bottle does not possess the function of carrying out the stirring to liquid medium simultaneously, can't guarantee the abundant contact of liquid medium and air.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a suspension cell culture bottle to solve the problem that proposes in the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme:

a suspension cell culture bottle comprises a bottle body, wherein a bottle cap is arranged at the bottle opening of the bottle body, the bottle cap is in threaded connection with the bottle opening of the bottle body, a bottom cap is arranged at the bottom end of the bottle body, the bottom cap is in threaded connection with the bottom end of the bottle body, a plurality of air inlet holes are formed in the outer side of the bottle body, a hydrophobic breathable film is fixedly connected to the inner wall of each air inlet hole and the inner wall of the bottle cap, a bottleneck is respectively arranged at two sides of the bottle body, the bottleneck is made of the same material as the bottle body and is integrally formed, one side of the bottle body is connected with a sample inlet through the bottleneck, the sample inlet is in threaded connection with the bottleneck, the other side of the bottle body is connected with a sample inlet through the bottleneck, the sample inlet is in threaded connection with the bottleneck, a, the bottle top is seted up and is used for the through-hole that the rotating rod top passed, the bottle includes outer bottle and inner bottle body, the fixed cover in the inner bottle body outside is equipped with outer bottle, be provided with the cavity between inner bottle body and the outer bottle body, the air intake and the one end of air-supply line of cavity are connected, and the air outlet and the one end of air-out pipe of cavity are connected.

As a further aspect of the present invention: the bottle cap top is provided with a plurality of holes.

As a further aspect of the present invention: the bottom of the bottle body is designed to be an opening.

As a further aspect of the present invention: the bottle is characterized in that one side of the bottle body is located at a position below the sample inlet and fixedly connected with a first ring, and the other side of the bottle body is located at a position below the sample inlet and fixedly connected with two second rings from top to bottom in sequence.

As a further aspect of the present invention: and anti-skid grains are arranged on the outer side of the top end of the rotating rod.

Compared with the prior art, the beneficial effects of the utility model are that: the top of the bottle cap is provided with a plurality of holes for facilitating the external air to enter the bottle body, so that the air in the bottle body is sufficient, the oxygen and the carbon dioxide required by cell production are provided, the bottom of the bottle body is designed to facilitate the operator to clean the inside of the bottle body when the bottle body is not used by adopting an opening, the practicability of the equipment is improved, the oxygen and the carbon dioxide required by cell growth are further ensured by arranging an air inlet for facilitating the air to enter the bottle body by adopting the opening, the hydrophobic breathable film is arranged for facilitating the air to enter the inside of the bottle body by the holes arranged at the top of the bottle cap and the air inlet, meanwhile, the liquid in the bottle body is prevented from leaking, the practicability of the equipment is improved, the sample introduction and the sampling in the bottle body are realized by arranging the sample inlet and the sample outlet, the operation is simple, when the bottle body needs to be shaken, the operator firstly stretches, further, the forefinger and the middle finger are respectively inserted into the two second finger rings, so that the first finger ring and the second finger ring are fixed on the fingers of the operator, the stability of the operator when the operator holds the bottle body by hands is improved, the situation that the operator slides when holding the bottle body by hands is avoided, the practicability of the equipment is improved, the anti-skid veins are arranged to increase the friction force between the hand of the operator and the outer side of the top end of the rotating rod, so that the operator can conveniently rotate the rotating rod and drive the stirring rod connected on the rotating rod to stir the liquid culture medium in the bottle body, the full contact between the liquid culture medium in the bottle body and the air is ensured, the practicability of the equipment is improved, when the temperature in the bottle body needs to be adjusted, hot air or cold air enters the cavity through the air inlet pipe, the quick adjustment of the temperature in the bottle body is realized, and the proper temperature in the bottle body is ensured, thereby ensuring the normal growth of cells and improving the practicability of the equipment.

Drawings

FIG. 1 is a schematic diagram of a suspension cell culture flask.

FIG. 2 is a schematic diagram of the structure of a flask in a suspension cell culture flask.

FIG. 3 is a partial structural view of a flask in a suspension cell culture flask.

Shown in the figure: the bottle comprises a bottle cap 1, an air inlet pipe 2, anti-skid lines 3, a through hole 4, a bottle body 5, an air inlet hole 6, a sample inlet 7, a first finger ring 8, a bottom cover 9, a stirring rod 10, a limiting cylinder 11, a rotating rod 12, a second finger ring 13, a sampling port 14, a hydrophobic breathable film 15, an air outlet pipe 16, an outer bottle body 17, an inner bottle body 18 and a cavity 19.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1 to 3, in an embodiment of the present invention, a suspension cell culture bottle includes a bottle cap 1, an air inlet pipe 2, anti-slip patterns 3, a through hole 4, a bottle body 5, an air inlet hole 6, an injection port 7, a first finger ring 8, a bottom cap 9, a stirring rod 10, a limiting cylinder 11, a rotating rod 12, a second finger ring 13, a sampling port 14, a hydrophobic breathable film 15, and an air outlet pipe 16, wherein the bottle cap 1 is disposed at a mouth of the bottle body 5, the bottle cap 1 is in threaded connection with the mouth of the bottle body 5, a plurality of holes are disposed at the top of the bottle cap 1, the plurality of holes are disposed at the top of the bottle cap 1 for facilitating external air to enter the bottle body 5, thereby ensuring sufficient air in the bottle body 5 and providing oxygen and carbon dioxide required for cell production, the bottom of the bottle body 5 is designed to be an opening, and the bottom of the bottle body 5 is designed for facilitating, the practicability of the equipment is improved, the bottom end of the bottle body 5 is provided with the bottom cover 9, the bottom cover 9 is in threaded connection with the bottom end of the bottle body 5, the outer side of the bottle body 5 is provided with a plurality of air inlet holes 6, the air is further convenient to enter the bottle body 5 through the air inlet holes 6, so that oxygen and carbon dioxide required by cell growth are further ensured, the inner walls of the air inlet holes 6 and the inner wall of the bottle cap 1 are fixedly connected with hydrophobic breathable films 15, the hydrophobic breathable films 15 are arranged to facilitate air to enter the bottle body 5 through the holes formed in the top of the bottle cap 1 and the air inlet holes 6, meanwhile, the liquid in the bottle body 5 is prevented from leaking, and the;

the bottle body 5 is provided with a bottleneck at two sides respectively, the bottleneck is made of the same material as the bottle body 5 and is integrally formed, one side of the bottle body 5 is connected with a sample inlet 7 through the bottleneck, the sample inlet 7 is in threaded connection with the bottleneck, the other side of the bottle body 5 is connected with a sample inlet 14 through the bottleneck, the sample inlet 14 is in threaded connection with the bottleneck, sample introduction and sampling in the bottle body 5 are realized through arranging the sample inlet 7 and the sample inlet 14, the operation is simple, a first finger ring 8 is fixedly connected at a position under the sample inlet 7 at one side of the bottle body 5, two second finger rings 13 are fixedly connected at a position under the sample inlet 14 at the other side of the bottle body 5 from top to bottom in sequence, when the bottle body 5 needs to be shaken, an operator firstly extends a thumb into the first finger ring 8, and further inserts a forefinger and a middle finger into the two second finger rings 13 respectively, thereby realizing the fixation of the first finger ring 8 and the second finger ring 13, the stability of the bottle body 5 when an operator holds the bottle body 5 is improved, the situation that the bottle body 5 is slid when the operator holds the bottle body 5 to shake is avoided, the practicability of the equipment is improved, the limiting cylinder 11 is fixedly arranged at the bottom of the inner side of the bottom cover 9, the top end of the limiting cylinder 11 is opened and the bottom end of the rotating rod 12 is arranged in the limiting cylinder, the rotating rod 12 is fixedly arranged with a plurality of stirring rods 10, when the bottom cover 9 is connected with the bottom end of the bottle body 5 in a threaded manner, the rotating rod 12 and the stirring rods 10 enter the bottle body 5, the top of the bottle body 5 is provided with a through hole 4 for the top end of the rotating rod 12 to pass through, the outer side of the top end of the rotating rod 12 is provided with anti-skid grains 3, the friction force between the hand of the operator and the outer side of the top end of the rotating rod 12 is increased through the anti-skid grains 3, thereby guarantee the abundant contact of liquid medium and air in the bottle 5, improved the practicality of equipment, the bottle 5 includes outer bottle 17 and inner bottle 18, the fixed cover in the inner bottle 18 outside is equipped with outer bottle 17, be provided with cavity 19 between inner bottle 18 and the outer bottle 17, the air intake and the one end of air-supply line 2 of cavity 19 are connected, and the air outlet and the one end that goes out tuber pipe 16 of cavity 19 are connected, when the temperature in the bottle 5 needs to be adjusted, make hot-blast or cold wind pass through in the air-supply line 2 gets into cavity 19 to realized the quick adjustment to the temperature in the bottle 5, ensured that the temperature is suitable in the bottle 5, thereby guaranteed the normal growth of cell, improved the practicality of equipment, the air in the cavity 19 further discharges through going out tuber pipe 16.

The utility model discloses a theory of operation is: the top of the bottle cap 1 is provided with a plurality of holes for facilitating the external air to enter the bottle body 5, so that the air in the bottle body 5 is sufficient, the oxygen and the carbon dioxide required by cell production are provided, the bottom of the bottle body 5 is designed to facilitate the cleaning of the inside of the bottle body 5 when the bottle is not used by an operator by adopting an opening, the practicability of the equipment is improved, the air is further facilitated to enter the bottle body 5 by arranging the air inlet hole 6, so that the oxygen and the carbon dioxide required by the cell growth are further ensured, the hydrophobic breathable film 15 is arranged for facilitating the air to enter the inside of the bottle body 5 through the holes arranged at the top of the bottle cap 1 and the air inlet hole 6 and avoiding the leakage of the liquid in the bottle body 5, the practicability of the equipment is improved, the sample introduction and the sampling in the bottle body 5 are realized by arranging the sample inlet 7 and the sample inlet 14, the operation is, an operator firstly extends a thumb into the first finger ring 8, and further inserts a forefinger and a middle finger into the two second finger rings 13 respectively, so that the fixing of the positions of the first finger ring 8 and the second finger ring 13 on the fingers of the operator is realized, the stability of the operator when holding the bottle body 5 by hand is improved, the condition that the operator slides down when holding the bottle body 5 by hand is avoided, and the practicability of the equipment is improved, when the bottom cover 9 is in threaded connection with the bottom end of the bottle body 5, the rotating rod 12 and the stirring rod 10 enter the bottle body 5, the friction force between the hand of the operator and the outer side of the top end of the rotating rod 12 is increased by arranging the anti-sliding lines 3, so that the operator can conveniently rotate the rotating rod 12 and drive the stirring rod 10 connected on the rotating rod 12 to rotate to stir the liquid culture medium in the bottle body 5, and the full contact between the liquid culture medium in the bottle body 5 and the air is, the practicality of equipment has been improved, when the temperature in the bottle 5 needs to be adjusted, make in hot-blast or cold wind gets into cavity 19 through air-supply line 2 to realized the quick adjustment to the temperature in the bottle 5, it is suitable to ensure the temperature in the bottle 5, thereby has guaranteed the normal growth of cell, has improved the practicality of equipment, and the air in the cavity 19 is further discharged through going out tuber pipe 16.

Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.

Claims

1. The suspension cell culture bottle comprises a bottle body (5) and is characterized in that a bottle cap (1) is arranged at the bottle opening of the bottle body (5), the bottle cap (1) is in threaded connection with the bottle opening of the bottle body (5), a bottom cover (9) is arranged at the bottom end of the bottle body (5), the bottom cover (9) is in threaded connection with the bottom end of the bottle body (5), a plurality of air inlet holes (6) are formed in the outer side of the bottle body (5), hydrophobic breathable films (15) are fixedly connected to the inner walls of the air inlet holes (6) and the inner wall of the bottle cap (1), a bottle neck is respectively arranged on each of two sides of the bottle body (5), the bottle neck and the bottle body (5) are made of the same material and are integrally formed, a sample inlet (7) is connected to one side of the bottle body (5) through the bottle neck, the sample, sample connection (14) and bottleneck threaded connection, bottom (9) inboard bottom fixed mounting has spacing section of thick bamboo (11), spacing section of thick bamboo (11) top opening and inside are provided with the bottom of rotating stick (12), it has a plurality of stirring rod (10) to rotate on stick (12), through-hole (4) that are used for rotating stick (12) top and pass are seted up at bottle (5) top, bottle (5) are including outer bottle (17) and inner bottle (18), the fixed cover in inner bottle (18) outside is equipped with outer bottle (17), be provided with cavity (19) between inner bottle (18) and the outer bottle (17), the air intake and the one end of air-supply line (2) of cavity (19) are connected, and the air outlet and the one end of air-out pipe (16) of cavity (19) are connected.

2. The suspension cell culture bottle according to claim 1, wherein the top of the bottle cap (1) is provided with a plurality of holes.

3. A suspension cell culture flask according to claim 1, characterized in that the bottom of the flask body (5) is open.

4. The suspension cell culture bottle according to claim 1 or 3, wherein a first finger ring (8) is fixedly connected to a position of one side of the bottle body (5) right below the sample inlet (7), and two second finger rings (13) are fixedly connected to positions of the other side of the bottle body (5) right below the sample inlet (14) from top to bottom.

5. The suspension cell culture flask according to claim 1, wherein the rotating rod (12) is provided with anti-slip lines (3) on the outside of the top end.