CN214628868U

CN214628868U - Plant tissue culture bottle

Info

Publication number: CN214628868U
Application number: CN202120967902.8U
Authority: CN
Inventors: 孙于佳; 孙子恒
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-05-08
Filing date: 2021-05-08
Publication date: 2021-11-09
Anticipated expiration: 2031-05-08

Abstract

The utility model belongs to plant tissue culture device field, concretely relates to plant tissue culture bottle, including upper end open-ended bottle, the bottle comprises last bottle and lower bottle, the lateral wall thickness of bottle and lower bottle is the same on its characterized in that, the lower extreme of going up the bottle is equipped with the bottle spacing collar, the outside of going up the bottle spacing collar flushes with last bottle outside, thickness is half of last bottle lateral wall thickness, lower bottle upper end is equipped with down the bottle spacing collar, the inboard of lower bottle spacing collar flushes with lower bottle inboard, thickness is half of lower bottle lateral wall thickness, goes up the bottle spacing collar and highly the same of lower bottle spacing collar, upper portion one side of bottle is equipped with the water seal groove down, goes up along highly being less than down the last height of bottle spacing collar in the water seal groove. The utility model has the advantages of reasonable structure, good sealing effect and convenient use.

Description

Plant tissue culture bottle

Technical Field

The utility model belongs to plant tissue culture device field, concretely relates to plant tissue culture bottle.

Background

Plant tissue culture (Plant tissue culture) is a technique in which isolated Plant organs (roots, stems, leaves, flowers, etc.), tissues (cambium, pollen, anthers, etc.), and cells are cultured on an artificially prepared culture medium under sterile conditions, and then cultured under appropriate culture conditions such as temperature and light, etc., to grow them into complete plants. With the continuous deepening of Chinese education reform and the comprehensive implementation of new lessons, plant tissue culture laboratories are established in many middle schools, and students can operate the laboratories by hands. The tissue culture bottle is a culture container for plant tissue growth and is a common experimental device in a plant tissue culture laboratory, the conventional tissue culture bottle is generally in a shape with a small bottle mouth and a thick and long bottle body, the small bottle mouth aims to reduce the contamination probability by reducing the contact area between the sterile environment in the bottle and the air outside the bottle, the defect is that the operation is inconvenient due to the small bottle mouth when a tissue block is inoculated and a bottle seedling is taken out of the bottle, in addition, when the bottle seedling is taken out of the bottle, the operation of peeling off the tissue culture seedling is difficult due to the small bottle mouth, the fragile tissue culture seedling is easily damaged, in order to solve the technical problem, a tissue culture bottle is invented by a technical person in the field, the publication number is CN108040878A, the publication date is 2018, 05 and 18 days, the bottle body is connected with the bottle bottom in a detachable threaded sealing mode, and the operation is convenient after the bottle body is separated from the bottle bottom. The applicant discovers that after studying the technology, because the bottle body and the bottle bottom are made of glass materials and the bottle body is thin in thickness, threads are machined on the surface of the glass, the difficulty is high, the precision is poor, the sealing effect of the combined part is poor after the bottle body and the bottle bottom are connected through the threads, external air can enter the bottle body and the bottle bottom along the gap, and the sterile environment in the bottle is damaged.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a plant tissue culture bottle, adopt the components of a whole that can function independently structure from top to bottom of its bottle, bottle processing is convenient, and the joint position utilizes the water seal to seal, and the air can't get into inside the bottle from the body upper and lower joint position, and is sealed effectual.

The utility model discloses a realize through following technical scheme:

the plant tissue culture bottle comprises a bottle body with an upper end opened, wherein the bottle body consists of an upper bottle body and a lower bottle body, the side wall thickness of the upper bottle body and the side wall thickness of the lower bottle body are the same, the lower end of the upper bottle body is provided with an upper bottle body limiting ring protruding downwards, the outer side of the upper bottle body limiting ring is flush with the outer side of the upper bottle body, the thickness is half of the thickness of the side wall of the upper bottle body, the upper end of the lower bottle body is provided with a lower bottle body limiting ring protruding upwards, the inner side of the lower bottle body limiting ring is flush with the inner side of the lower bottle body, the thickness is half of the thickness of the side wall of the lower bottle body, the upper bottle body limiting ring and the lower bottle body limiting ring are the same in height, a water seal groove is arranged on one side of the upper portion of the lower bottle body, the upper edge of the water seal groove is lower than the upper edge height of the lower bottle body limiting ring, and the inner bottom surface of the water seal groove is flush or lower end of the upper bottle body limiting ring.

The utility model discloses an it links together convenient operation to adopt the mode of going up bottle spacing collar and lower bottle spacing collar suit between the bottle and the lower bottle.

The utility model discloses an go up and adopt prior art suppression or blowing technology integrated into one piece between the bottle body spacing collar and the last bottle body, go up and adopt prior art suppression or blowing technology integrated into one piece between bottle body spacing collar and the last bottle body. The processing is convenient, and the matching precision of the upper bottle body limiting ring and the lower bottle body limiting ring is high.

The utility model discloses an inside bottom surface of water seal groove flushes or is less than the lower extreme of last bottle spacing collar, and the last edge of water seal groove is less than the upper end of bottle spacing collar down, pours into the water back of take the altitude into at the water seal inslot, and water is sealed up the lower extreme of bottle spacing collar completely for the air can't get into inside the bottle through last bottle and lower bottle combination position.

The utility model discloses an going up of water seal groove along must not being higher than the upper end of lower bottle spacing collar, prevents that the water in the water seal from getting into inside the bottle under the atmospheric pressure effect.

The utility model discloses a bottleneck is the tang design, and the bottleneck is provided with the ventilated membrane or has the sealed cap of ventilated membrane. The breathable film or the sealing cap is a product in the prior art.

Further, the utility model discloses a lower extreme of going up the bottle spacing collar and the upper end of bottle spacing collar down are equipped with bottle sealing washer and lower bottle sealing washer respectively, and the thickness of going up bottle sealing washer and lower bottle sealing washer is the same.

By using the sealing ring, the sealing effect between the upper bottle body and the lower bottle body is better.

Furthermore, an annular water seal groove cover is arranged on the outer side of the upper bottle body, the inner diameter of the water seal groove cover is matched with the outer diameter of the upper bottle body, and the outer diameter of the water seal groove cover is equal to or larger than the outer diameter of the water seal groove.

The water seal groove cover is made of plastic/silica gel/rubber, is sleeved on the outer side of the upper bottle body and covers the upper end of the water seal groove, so that water in the water seal groove can be prevented from evaporating too fast, and the water seal effect is ensured.

The utility model has the advantages of reasonable structure and convenient operation. Use the utility model discloses, with upper and lower bottle separation can be convenient put into nutrition base and tissue piece, utilize water to seal after upper and lower bottle combination, it is sealed effectual, when the bottle seedling goes out the bottle, with upper and lower bottle separately, can be convenient, harmless take out the tissue culture seedling from the bottle.

Drawings

Fig. 1 is a schematic structural view of the present invention;

fig. 2 is a schematic sectional structure of the present invention;

fig. 3 is an enlarged schematic view of a portion a in fig. 2.

As shown in the figure: 1. a bottle mouth; 2. the bottle body is arranged; 3. a water seal tank cover; 4. water sealing the tank; 5. discharging the bottle body; 6. an upper bottle body limiting ring; 7. a lower bottle body sealing ring; 8. a lower bottle body limiting ring; 9. and an upper bottle body sealing ring.

Detailed Description

Embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and therefore are only examples, and the protection scope of the present invention is not limited thereby. It is to be noted that unless otherwise specified, technical or scientific terms used herein shall have the ordinary meaning as understood by those skilled in the art to which the present invention belongs.

As shown in fig. 1, 2, and 3: the bottle body consists of an upper bottle body 2 and a lower bottle body 5, the side wall thicknesses of the upper bottle body 2 and the lower bottle body 5 are the same, the height of the upper bottle body 2 accounts for 2/3 of the height of the whole bottle body, the bottle opening at the upper end of the bottle body is a narrow opening, the lower end of the upper bottle body 2 is provided with an upper bottle body limiting ring 6 which protrudes downwards, the outer side of the upper bottle body limiting ring 6 is flush with the outer side of the upper bottle body 2, the wall thickness is 1/2 of the bottle body thickness, the upper end of the lower bottle body 5 is provided with a lower bottle body limiting ring 8 which protrudes upwards, the inner side of the lower bottle body limiting ring 8 is flush with the inner side of the lower bottle body 5, the wall thickness is 1/2 of the bottle body wall thickness, the outer side of the upper end of the lower bottle body 5 is provided with an annular water sealing groove 4 with an upper opening, the inner bottom surface of the water sealing groove 4 is flush with the lower end of the upper bottle body limiting ring 6, the upper edge of the side wall of the water sealing groove 4 is lower bottle body limiting ring 8, the outer part of the upper bottle body 2 is sleeved with an annular water sealing groove cover 3, the inner diameter of the water seal groove cover 3 is matched with the outer diameter of the upper bottle body 2, the outer diameter is larger than the outer diameter of the water seal groove 4, and the water seal groove cover covers the upper end of the water seal groove 4.

The utility model discloses during the use, will go up bottle 2 and lower bottle 5 separation, directly put into nutrition base and tissue piece in lower bottle 5, will go up bottle 2 and install on bottle 5, set up the ventilated membrane or have the sealed lid of ventilated membrane in the bottleneck department of last bottle 2, inject water in water seal tank 4, cover water seal capping 3 in water seal tank 4 upper end, can carry out the cultivation of tissue piece. After the culture is finished, when the seedlings are taken out of the bottle, the upper bottle body 2 and the lower bottle body 5 are separated, and the tissue culture seedlings can be conveniently and nondestructively taken out of the lower bottle body 5.

The above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; such modifications and substitutions do not substantially depart from the scope of the embodiments of the present invention, and are intended to be covered by the claims and the specification.

Claims

1. The utility model provides a plant tissue culture bottle, includes upper end open-ended bottle, and the bottle comprises last bottle and lower bottle, and the lateral wall thickness of bottle is the same with lower bottle in its characterized in that, and the lower extreme of going up the bottle is equipped with the bottle spacing collar, and the outside of going up the bottle spacing collar flushes with last bottle outside, and thickness is half of last bottle lateral wall thickness, and lower bottle upper end is equipped with down the bottle spacing collar, and the inboard of lower bottle spacing collar flushes with lower bottle inboard, and thickness is half of lower bottle lateral wall thickness, goes up the bottle spacing collar and highly the same of lower bottle spacing collar, the upper portion one side of bottle is equipped with the water seal groove down, and the last edge height that highly is less than down the bottle spacing collar of water seal groove.

2. The plant tissue culture bottle according to claim 1, wherein the upper and lower bottle body sealing rings are provided at the lower end of the upper bottle body stopper ring and the upper end of the lower bottle body stopper ring, respectively, and have the same thickness.

3. The plant tissue culture bottle according to claim 1, wherein the outer side of the upper bottle body is provided with an annular water seal groove cover, the inner diameter of the water seal groove cover matches with the outer diameter of the upper bottle body, and the outer diameter of the water seal groove cover is equal to or larger than the outer diameter of the water seal groove.