CN209128460U - A kind of culture apparatus can be used for continuous perfusion culture stem cell - Google Patents
A kind of culture apparatus can be used for continuous perfusion culture stem cell Download PDFInfo
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- CN209128460U CN209128460U CN201821818681.2U CN201821818681U CN209128460U CN 209128460 U CN209128460 U CN 209128460U CN 201821818681 U CN201821818681 U CN 201821818681U CN 209128460 U CN209128460 U CN 209128460U
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- incubator
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- continuous perfusion
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Abstract
The utility model provides a kind of culture apparatus that can be used for continuous perfusion culture stem cell, including incubator, end cap is provided at the top of the incubator, middle position at the top of the end cap is provided with operation port, hinge is provided at the top of operation port at the top of the end cap, capping is provided on the hinge, the specification of the capping is adapted with the specification of operation port, and pull ring is provided at the top of capping, the incubator is internally provided with incubator liner, the inside of the incubator liner is contained with culture solution, the bottom of incubator side outer wall is provided with liquid outlet, and the inside of liquid outlet is plugged with the first silicone tube, the utility model is conducive to detect the environmental factor inside incubator, structure novel, design is reasonable, it can continuously realize the perfusion to stem cell, it improves pure when stem cell is cultivated Degree enhances quality when stem cell uses, substantially increases the efficiency of stem cell culture.
Description
Technical field
The utility model relates to technical field of stem cell culture, and in particular to one kind can be used for continuous perfusion culture stem cell
Culture apparatus.
Background technique
Stem cell refers to the initial cell with self-renewing and multi-lineage potential, is the cells of origin of body, in spy
The cell as one or more composition tissues or organ can be broken up under conditions of fixed.Self-renewing refers to that stem cell can
The size of stem cell population is maintained by cell division；Multidirectional Differentiation refers to that stem cell has plasticity, in different environments,
Different histocytes (such as mescenchymal stem cell can break up neuroblast, osteoblast) can be divided into constitute
The histoorgan of the various complexity of body is being cultivated currently, generalling use traditional T bottles for the culture of the treatments such as stem cell cell
Static batch culture in case.
Traditional T bottles of only one operation port cannot mend in time without this structure setting of two logical culture solution inlet and outlet
It fills nutrients and removes metabolin, be unfavorable for the High Density Cultivation of cell.Also, T bottles of tradition cannot online grasp cell
Make, such as online enzymic digestion, piping and druming, sampling and amplification, it is poor to the Environmental Control Effect of cell culture, it seriously affects and cell is trained
Feeding quality.Therefore, need to design a kind of culture apparatus that can be used for continuous perfusion culture stem cell to solve the above problems.
Utility model content
In view of the deficienciess of the prior art, the purpose of this utility model is to provide one kind can be used for continuous perfusion culture
The culture apparatus of stem cell, it is especially a kind of to can be used for continuous perfusion culture stem cell, the culture environment control effect to cell
Good culture apparatus.
To achieve the goals above, the utility model provides the following technical solutions: it is dry that one kind can be used for continuous perfusion culture
The culture apparatus of cell, including incubator are provided with end cap at the top of the incubator, and the middle position at the top of the end cap is opened
There is operation port, is provided with hinge at the top of operation port at the top of the end cap, capping, the envelope are provided on the hinge
The specification of blanking cover is adapted with the specification of operation port, and pull ring is provided at the top of capping, the inside setting of the incubator
There is incubator liner, the inside of the incubator liner is contained with culture solution, and the bottom of incubator side outer wall is provided with out
Liquid mouth, and the inside of liquid outlet is plugged with the first silicone tube, first silicone tube are internally provided with filter device, and described
One silicone tube is provided with collecting box far from one end of incubator, is provided with liquid inlet at the top of the outer wall of the incubator side, and enter
Liquid mouth is internally provided with the second silicone tube, and second silicone tube is provided with feed box far from one end of incubator.
Further, the filter device is internally provided with filter membrane, and filter membrane is fiber membrane, fiber membrane
Aperture is micron.
Further, it is provided with waste liquid outlet on the outer wall of the incubator bottom side, and is provided on waste liquid outlet useless
Liquid pipe.
Further, it is provided with mounting rack on the side inner wall of the incubator liner, is respectively set on the mounting rack
There are humidity sensor and temperature sensor, and humidity sensor and temperature sensor are located at the liquid level of culture solution or less.
Further, the protrusion equidistantly distributed, the incubator are provided on the inner wall of the incubator liner at the bottom
Albumin coated layer is provided on the inner wall of inner container side surface.
Further, the side at the top of the end cap is provided with mounting hole, and mounting hole is internally provided with propeller, described
Propeller is collectively formed by sealing shell and mobile plunger, and the sealing shell is mounted on the inside of mounting hole, and mobile plunger
It is plugged on the inside of sealing shell.
Further, it is provided with air vent on the side outer wall of the sealing shell, and is provided with and leads on the inner wall of air vent
Tracheae, gas-guide tube are connected with gas tank far from one end of sealing shell.
Compared with prior art, the utility model has the beneficial effects that
1, by the temperature sensor of setting and humidity sensor, be conducive to control the environmental factor inside incubator
System, ensure that the normal habitat of stem cell, substantially increase the efficiency of stem cell culture.
2, since protrusion is evenly distributed on container bottom, stem cell assembles on a small quantity in the bottom of incubator, connects with culture solution
Touching more evenly, by the albumin coated layer of setting, to greatly reduce stem cell in the residual rate that can found culture vessel, has
Conducive to preventing, stem cell is adherent, maintains the suspended state of neural stem cell.
3, by the incubator of setting, separator box and collecting box, the perfusion to stem cell can be continuously realized, reaching can
Continuously to the effect of stem cell culture.
4, by the filter device of setting, filter membrane and fiber membrane, it can be filtered operation to stem cell, improve
Purity when stem cell is cultivated enhances quality when stem cell uses.
Detailed description of the invention
Fig. 1 be the utility model proposes a kind of culture apparatus that can be used for continuous perfusion culture stem cell overall structure
Perspective view.
Fig. 2 be the utility model proposes a kind of culture apparatus that can be used for continuous perfusion culture stem cell structure section view
Figure.
Fig. 3 be the utility model proposes a kind of culture apparatus that can be used for continuous perfusion culture stem cell filter device
Structural schematic diagram.
Fig. 4 be the utility model proposes a kind of culture apparatus that can be used for continuous perfusion culture stem cell propeller knot
Structure schematic diagram.
Main element symbol description: 1- incubator；2- operation port；3- hinge；4- capping；5- incubator liner；6- training
Nutrient solution；The first silicone tube of 7-；8- filter device；9- collecting box；10- feed box；The second silicone tube of 11-；12- mounting rack；13- is wet
Spend sensor；14- temperature sensor；15- end cap；16- albumin coated layer；17- protrusion；18- filter membrane；19- sewer pipe；
20- sealing shell；21- moves plunger；22- gas tank.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
Every other embodiment obtained, fall within the protection scope of the utility model.
It should be noted that it can be directly on another component when component is referred to as " being fixed on " another component
Or there may also be components placed in the middle.When a component is considered as " connection " another component, it, which can be, is directly connected to
To another component or it may be simultaneously present component placed in the middle.When a component is considered as " being set to " another component, it
It can be and be set up directly on another component or may be simultaneously present component placed in the middle.Term as used herein is " vertical
", " horizontal ", "left", "right" and similar statement for illustrative purposes only.
Unless otherwise defined, all technical and scientific terms used herein are led with the technology for belonging to the utility model
The normally understood meaning of the technical staff in domain is identical.Terminology used in the description of the utility model herein only be
The purpose of description specific embodiment, it is not intended that in limitation the utility model.Term " and or " used herein includes
Any and all combinations of one or more related listed items.
Please referring also to Fig. 1 to Fig. 4, a kind of culture apparatus can be used for continuous perfusion culture stem cell, including incubator
1, the top of the incubator 1 is provided with end cap 15, and end cap 15 is used with incubator 1 and is threadedly coupled, 15 top of end cap
Middle position is provided with operation port 2, and operation port 2 is completed to carry out online enzymic digestion, piping and druming, sampling, amplification, the end cap to cell
15 tops are provided with hinge 3 close to the top of operation port 2, are provided with capping 4 on the hinge 3, by the rotation of hinge 3,
Convenient for the unlatching and closure to capping 4, the specification of the capping 4 is adapted with the specification of operation port 2, and capping 4
Top is provided with pull ring, and the incubator 1 is internally provided with incubator liner 5, and the inside of the incubator liner 5 is contained with
The bottom of culture solution 6, the 1 side outer wall of incubator is provided with liquid outlet, and the inside of liquid outlet is plugged with the first silicone tube 7,
First silicone tube 7 is internally provided with filter device 8, and first silicone tube 7 is provided with receipts far from one end of incubator 1
Header 9, collecting box 9 can store the stem cell that culture is completed, be provided at the top of the 1 side outer wall of incubator into liquid
Mouthful, and liquid inlet is internally provided with the second silicone tube 11, second silicone tube 11 far from one end of incubator 1 be provided with into
Hopper 10 can store the stoste of stem cell.
Further, the filter device 8 is internally provided with filter membrane 18, and filter membrane 18 is fiber membrane, fiber
The aperture of film is 200 microns, achievees the effect that filtering, can guarantee the purity of stem cell culture.
Further, it is provided with waste liquid outlet on the outer wall of 1 bottom side of incubator, and is provided on waste liquid outlet useless
Liquid pipe 19, the waste liquid discharge after stem cell is cultivated, and cleaning of the operator to incubator can be convenient for.
Further, it is provided with mounting rack 12 on the side inner wall of the incubator liner 5, distinguishes on the mounting rack 12
It is provided with humidity sensor 13 and temperature sensor 14, and humidity sensor 13 and temperature sensor 14 are located at the liquid of culture solution 6
Face is hereinafter, humidity sensor 13 and temperature sensor 14 can be convenient the temperature and humidity inside detection incubator 1.
Further, the protrusion 17 equidistantly distributed, the culture are provided on the inner wall of 5 bottom of incubator liner
Albumin coated layer 16 is provided on the inner wall of 5 side of case liner, it is semicircle or semiellipse type, albumen packet that protrusion 17, which is cross section,
Tegillum 16 is human serum albumin coated layer, and albumin coated layer 16 is attached to incubator in a manner of physical absorption or covalent cross-linking
On 1 inner wall, by the setting of protrusion 17, the structure that traditionally incubator bottom is plane has been abandoned, and protrusion 17 increases
The surface area of incubator bottom makes it be more suitable for the culture of stem cell, accelerates the growth rate of stem cell, at the same reduce culture,
Loss during storage.
Further, the side at 15 top of the end cap is provided with mounting hole, and mounting hole is internally provided with propeller, institute
Propeller to be stated to be collectively formed by sealing shell 20 and mobile plunger 21, the sealing shell 20 is mounted on the inside of mounting hole, and
Mobile plunger 21 is plugged on the inside of sealing shell 20, enhances the pressure inside incubator 1, structure and drop needle in the market
The principle of pipe is identical, facilitates the progress filtered to stem cell, while the stem cell that culture is completed is transferred in collecting box 9
Portion.
Further, it is provided with air vent on the side outer wall of the sealing shell 20, and is provided on the inner wall of air vent
Gas-guide tube, gas-guide tube are connected with gas tank 22 far from one end of sealing shell 20, and gas tank 22 is the clean gas of propeller conveying, and
It will not influence the culture environment of stem cell.
Working principle: operator is added to the culture stoste of stem cell from the inside of feed box 10 inside of incubator 1,
It is grown and is bred so that stem cell is in the culture solution 6 inside incubator liner 5, while operator can open closure
Lid 4 completes online enzymic digestion, piping and druming, sampling, the amplification to stem cell, temperature sensor 14 and humidity in the inside of operation port 2
Sensor 13 detects the temperature and humidity inside incubator 1, and after completing to stem cell culture, operator passes through shifting
The dynamic mobile plunger 21 inside sealing shell 20, so that the pressure inside incubator 1 increases, by filter device 8 training
It supports the stem cell completed to be separated, so that the stem cell that culture is completed enters the inside of collecting box 9, complete continuously to dry
The culture of cell operates, and remaining waste liquid discharge the outside of incubator 1 by sewer pipe 19.
Above description is the detailed description for the preferable possible embodiments of the utility model, but embodiment is not limited to
The patent claim of the utility model, the same changes or modifications completed under technical spirit suggested by all the utility model
Change, should belong to the covered the scope of the patents of the utility model.
Claims (7)
1. a kind of culture apparatus that can be used for continuous perfusion culture stem cell, which is characterized in that including incubator (1), the training
It supports and is provided with end cap (15) at the top of case (1), the middle position at the top of the end cap (15) is provided with operation port (2), the end cap
(15) top is provided with hinge (3) at the top of operation port (2), is provided with capping (4), the envelope on the hinge (3)
The specification of blanking cover (4) is adapted with the specification of operation port (2), and pull ring, the incubator are provided at the top of capping (4)
(1) be internally provided with incubator liner (5), the inside of the incubator liner (5) is contained with culture solution (6), the culture
The bottom of case (1) side outer wall is provided with liquid outlet, and the inside of liquid outlet is plugged with the first silicone tube (7), first silica gel
Pipe (7) is internally provided with filter device (8), and first silicone tube (7) is provided with collecting box far from the one end of incubator (1)
(9), it is provided with liquid inlet at the top of the outer wall of incubator (1) side, and liquid inlet is internally provided with the second silicone tube (11),
Second silicone tube (11) is provided with feed box (10) far from the one end of incubator (1).
2. a kind of culture apparatus that can be used for continuous perfusion culture stem cell according to claim 1, which is characterized in that institute
Being internally provided with filter membrane (18) for filter device (8) is stated, and filter membrane (18) is fiber membrane, the aperture of fiber membrane is
200 microns.
3. a kind of culture apparatus that can be used for continuous perfusion culture stem cell according to claim 1, which is characterized in that institute
It states and is provided with waste liquid outlet on the outer wall of incubator (1) bottom side, and be provided with sewer pipe (19) on waste liquid outlet.
4. a kind of culture apparatus that can be used for continuous perfusion culture stem cell according to claim 1, which is characterized in that institute
It states and is provided on the side inner wall of incubator liner (5) mounting rack (12), humidity biography is respectively arranged on the mounting rack (12)
Sensor (13) and temperature sensor (14), and humidity sensor (13) and temperature sensor (14) are located at the liquid level of culture solution (6)
Below.
5. a kind of culture apparatus that can be used for continuous perfusion culture stem cell according to claim 1, which is characterized in that institute
The protrusion (17) for being provided with and equidistantly distributing on the inner wall of incubator liner (5) bottom is stated, incubator liner (5) side
Albumin coated layer (16) are provided on inner wall.
6. a kind of culture apparatus that can be used for continuous perfusion culture stem cell according to claim 1, which is characterized in that institute
It states the side at the top of end cap (15) and is provided with mounting hole, and mounting hole is internally provided with propeller, the propeller is outer by sealing
Shell (20) and mobile plunger (21) collectively form, and the sealing shell (20) is mounted on the inside of mounting hole, and mobile plunger
(21) it is plugged on the inside of sealing shell (20).
7. a kind of culture apparatus that can be used for continuous perfusion culture stem cell according to claim 6, which is characterized in that institute
It states and is provided with air vent on the side outer wall of sealing shell (20), and be provided with gas-guide tube on the inner wall of air vent, gas-guide tube is separate
One end of sealing shell (20) is connected with gas tank (22).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821818681.2U CN209128460U (en) | 2018-11-06 | 2018-11-06 | A kind of culture apparatus can be used for continuous perfusion culture stem cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821818681.2U CN209128460U (en) | 2018-11-06 | 2018-11-06 | A kind of culture apparatus can be used for continuous perfusion culture stem cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209128460U true CN209128460U (en) | 2019-07-19 |
Family
ID=67244047
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821818681.2U Active CN209128460U (en) | 2018-11-06 | 2018-11-06 | A kind of culture apparatus can be used for continuous perfusion culture stem cell |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209128460U (en) |
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2018
- 2018-11-06 CN CN201821818681.2U patent/CN209128460U/en active Active
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