CN206418144U - A kind of permeability type lymphocyte blake bottle - Google Patents
A kind of permeability type lymphocyte blake bottle Download PDFInfo
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- CN206418144U CN206418144U CN201720041247.7U CN201720041247U CN206418144U CN 206418144 U CN206418144 U CN 206418144U CN 201720041247 U CN201720041247 U CN 201720041247U CN 206418144 U CN206418144 U CN 206418144U
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- 210000004698 lymphocyte Anatomy 0.000 title claims abstract description 11
- 230000035699 permeability Effects 0.000 title claims 4
- 239000004816 latex Substances 0.000 claims abstract description 13
- 229920000126 latex Polymers 0.000 claims abstract description 13
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims abstract description 8
- 229910052782 aluminium Inorganic materials 0.000 claims abstract description 8
- 239000011888 foil Substances 0.000 claims abstract description 8
- 239000012528 membrane Substances 0.000 claims abstract description 7
- 238000005119 centrifugation Methods 0.000 claims 1
- 239000003550 marker Substances 0.000 claims 1
- 239000001963 growth medium Substances 0.000 abstract description 15
- 238000000034 method Methods 0.000 abstract description 7
- 238000007789 sealing Methods 0.000 abstract description 7
- 238000011081 inoculation Methods 0.000 abstract description 6
- 244000005700 microbiome Species 0.000 abstract description 4
- 241000894006 Bacteria Species 0.000 abstract description 3
- 238000011109 contamination Methods 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 210000004976 peripheral blood cell Anatomy 0.000 abstract description 3
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 238000004113 cell culture Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000013013 elastic material Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
本实用新型公开了一种透气式淋巴细胞培养瓶，包括瓶体，瓶体的顶部设有相配合的瓶盖，瓶体是下端设有一尖端的细长离心管式结构，瓶体的瓶口处设有铝箔封口垫片，瓶盖上设有透气膜和乳胶孔，透气膜上设有若干透气孔。本实用新型的瓶盖上设有乳胶孔，可以方便接种，避免了细菌污染培养基；本实用新型的瓶盖上设有透气膜，即可以防止其它微生物进入培养瓶内，污染培养基，又可以使外周血细胞在培养过程中，可以与外界环境相连通，达到透气的效果；本实用新型的刻度标识线可以对培养瓶中的培养基的含量起到标识作用；本实用新型的铝箔封口垫片可以防止运输过程中培养基沾染到瓶口或者透气滤膜上，造成培养基染菌，使用前将其抽出即可。
The utility model discloses a breathable lymphocyte culture bottle, which comprises a bottle body, the top of the bottle body is provided with a matching bottle cap, the bottle body is a slender centrifuge tube structure with a tip at the lower end, the bottle mouth of the bottle body There is an aluminum foil sealing gasket at the place, a breathable film and latex holes are provided on the bottle cap, and a number of air holes are provided on the breathable film. The bottle cap of the utility model is provided with a latex hole, which can facilitate inoculation and avoid bacterial contamination of the culture medium; the bottle cap of the utility model is provided with a breathable film, which can prevent other microorganisms from entering the culture bottle and contaminate the culture medium. During the culture process, the peripheral blood cells can be connected with the external environment to achieve a breathable effect; the scale marking line of the utility model can mark the content of the culture medium in the culture bottle; the aluminum foil sealing pad of the utility model The sheet can prevent the culture medium from contaminating the bottle mouth or the air-permeable filter membrane during transportation, causing the culture medium to be contaminated with bacteria, just pull it out before use.
Description
技术领域technical field
本实用新型涉及生物技术领域，具体来说，涉及一种透气式淋巴细胞培养瓶。The utility model relates to the field of biotechnology, in particular to a gas-permeable lymphocyte culture bottle.
背景技术Background technique
细胞培养需要无微生物污染环境，即需要对所需细胞进行无菌的隔离培养，以避免环境例如空气中的各种微生物进入细胞培养体系，导致微生物污染。现有的淋巴细胞培养瓶在接种时，需要拧开螺旋瓶盖，而开闭瓶盖的这一过程中不可避免的会增加污染培养基的机率。Cell culture requires an environment free of microbial contamination, that is, the desired cells need to be cultured in a sterile isolation environment to prevent various microorganisms in the environment such as the air from entering the cell culture system and causing microbial contamination. When the existing lymphocyte culture bottle is inoculated, the screw bottle cap needs to be unscrewed, and the process of opening and closing the bottle cap will inevitably increase the probability of contaminating the culture medium.
实用新型内容Utility model content
针对相关技术中的上述技术问题，本实用新型提出一种透气式淋巴细胞培养瓶，能够克服现有技术的上述不足。Aiming at the above-mentioned technical problems in the related art, the utility model proposes a gas-permeable lymphocyte culture bottle, which can overcome the above-mentioned deficiencies in the prior art.
为实现上述技术目的，本实用新型的技术方案是这样实现的：For realizing above-mentioned technical purpose, technical scheme of the present utility model is realized like this:
一种透气式淋巴细胞培养瓶，包括瓶体，所述的瓶体的顶部设有相配合的瓶盖，所述的瓶体是下端设有一尖端的细长离心管式结构，所述的瓶体的瓶口处设有铝箔封口垫片，所述的瓶盖上设有透气膜和乳胶孔，所述的透气膜上设有若干透气孔，所述的透气孔的孔径为0.22微米。A gas-permeable lymphocyte culture bottle, including a bottle body, the top of the bottle body is provided with a matching bottle cap, and the bottle body is a slender centrifuge tube structure with a tip at the lower end. The bottle mouth of the body is provided with an aluminum foil sealing gasket, the bottle cap is provided with a breathable film and latex holes, the breathable film is provided with a number of air holes, and the diameter of the air holes is 0.22 microns.
进一步的，所述的瓶体上设有刻度标识线。Further, the bottle body is provided with scale marking lines.
优选的，所述的乳胶孔位于瓶盖上壁的中央。Preferably, the latex hole is located at the center of the upper wall of the bottle cap.
本实用新型的有益效果：本实用新型的瓶盖上设有乳胶孔，可以方便接种，避免了由于开闭瓶盖这一过程增加的污染培养基的机率；本实用新型的瓶盖上设有透气膜，即可以防止其它微生物进入培养瓶内，污染培养基，又可以使外周血细胞在培养过程中，可以与外界环境相连通，达到透气的效果；本实用新型的刻度标识线可以对培养瓶中的培养基的含量起到标识作用；本实用新型的铝箔封口垫片可以防止运输过程中培养基沾染到瓶口或者透气滤膜上，造成培养基染菌，使用前将其抽出即可。Beneficial effects of the present utility model: the bottle cap of the present utility model is provided with latex holes, which can facilitate inoculation, and avoids the probability of polluting the culture medium due to the process of opening and closing the bottle cap; the bottle cap of the present utility model is provided with The gas-permeable film can prevent other microorganisms from entering the culture bottle and contaminate the culture medium, and can make the peripheral blood cells communicate with the external environment during the culture process to achieve a breathable effect; The content of the culture medium in the medium plays the role of identification; the aluminum foil sealing gasket of the utility model can prevent the culture medium from contaminating the bottle mouth or the air-permeable filter membrane during transportation, causing the culture medium to be contaminated with bacteria, and it can be pulled out before use.
附图说明Description of drawings
为了更清楚地说明本实用新型实施例或现有技术中的技术方案，下面将对实施例中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图仅仅是本实用新型的一些实施例，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据这些附图获得其他的附图。In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings required in the embodiments will be briefly introduced below. Obviously, the accompanying drawings in the following description are only the present invention. For some embodiments of the present invention, those of ordinary skill in the art can also obtain other drawings based on these drawings on the premise of not paying creative efforts.
图1是根据本实用新型实施例所述的一种透气式淋巴细胞培养瓶的结构示意图；Fig. 1 is a schematic structural view of a gas-permeable lymphocyte culture bottle according to an embodiment of the present invention;
图2是根据本实用新型实施例所述的瓶盖的俯视图；Fig. 2 is a top view of the bottle cap according to the embodiment of the present invention;
图中：1、刻度标识线；2、铝箔封口垫片；3、透气膜；4、乳胶孔；5、瓶体；6、尖端；7、瓶盖。In the figure: 1. Scale marking line; 2. Aluminum foil sealing gasket; 3. Breathable membrane; 4. Latex hole; 5. Bottle body; 6. Tip; 7. Bottle cap.
具体实施方式detailed description
下面将结合本实用新型实施例中的附图，对本实用新型实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本实用新型一部分实施例，而不是全部的实施例。基于本实用新型中的实施例，本领域普通技术人员所获得的所有其他实施例，都属于本实用新型保护的范围。The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. example. All other embodiments obtained by persons of ordinary skill in the art based on the embodiments of the present invention belong to the protection scope of the present invention.
如图1-2所示，根据本实用新型实施例所述的一种透气式淋巴细胞培养瓶，包括瓶体5，所述的瓶体5的顶部配合连接有瓶盖7，所述的瓶体5是下端设有一尖端6的细长离心管式结构，所述的瓶体5的瓶口处设有铝箔封口垫片，所述的瓶盖7上设有透气膜3和乳胶孔4，所述的透气膜3上设有若干透气孔，所述的透气孔的孔径为0.22微米。其中，所述的瓶体的上部设有外螺纹，所述的瓶盖的侧壁上设有内螺纹，所述的瓶体5通过内螺纹与外螺纹配合与瓶盖7连接。As shown in Figure 1-2, a gas-permeable lymphocyte culture bottle according to the embodiment of the present utility model includes a bottle body 5, the top of the bottle body 5 is fitted with a bottle cap 7, and the bottle body 5 is The body 5 is a slender centrifuge tube structure with a tip 6 at the lower end. The bottle mouth of the bottle body 5 is provided with an aluminum foil sealing gasket, and the bottle cap 7 is provided with a gas-permeable film 3 and a latex hole 4. The air-permeable membrane 3 is provided with a number of air-ventilating holes, and the diameter of the air-permeable holes is 0.22 microns. Wherein, the upper part of the bottle body is provided with an external thread, and the side wall of the bottle cap is provided with an internal thread, and the bottle body 5 is connected with the bottle cap 7 through the cooperation of the internal thread and the external thread.
在一具体实施例中，所述的乳胶孔4位于瓶盖7上壁的中央。In a specific embodiment, the latex hole 4 is located at the center of the upper wall of the bottle cap 7 .
在一具体实施例中，所述的瓶体5上设有刻度标识线1。In a specific embodiment, the bottle body 5 is provided with a scale marking line 1 .
在一具体实施例中，所述的乳胶孔4位于瓶盖7上壁的中央。In a specific embodiment, the latex hole 4 is located at the center of the upper wall of the bottle cap 7 .
在具体使用时，本实用新型的瓶盖上设有乳胶孔，可以方便接种，在接种时注射器针头直接进入其中，接种后抽出针头，乳胶孔由于其弹性材质而自动闭合。此设计即可以使接种操作简便，无需拧开螺旋瓶盖，直接进出接种针头，又避免了由于开闭瓶盖这一过程增加的污染培养基的机率；本实用新型的瓶盖上设有透气膜，即可以防止其它微生物进入培养瓶内，污染培养基，又可以使外周血细胞在培养过程中，可以与外界环境相连通，达到透气的效果；本实用新型的刻度标识线可以对培养瓶中的培养基的含量起到标识作用；本实用新型的铝箔封口垫片可以防止运输过程中培养基沾染到瓶口或者透气滤膜上，造成培养基染菌，使用前将其抽出即可。In specific use, the bottle cap of the present utility model is provided with latex holes, which can facilitate inoculation. When inoculating, the needle of the syringe directly enters it, and the needle is drawn out after inoculation, and the latex holes are automatically closed due to its elastic material. This design can make the inoculation operation easy, without unscrewing the screw cap, and the inoculation needle can directly enter and exit, and avoids the probability of polluting the medium due to the process of opening and closing the cap; the cap of the utility model is equipped with a ventilating The film can prevent other microorganisms from entering the culture bottle and pollute the culture medium, and can make the peripheral blood cells communicate with the external environment during the culture process, so as to achieve the effect of ventilation; The content of the culture medium plays the role of identification; the aluminum foil sealing gasket of the utility model can prevent the culture medium from contaminating the bottle mouth or the air-permeable filter membrane during transportation, causing the culture medium to be contaminated with bacteria, and it can be pulled out before use.
以上所述仅为本实用新型的较佳实施例而已，并不用以限制本实用新型，凡在本实用新型的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本实用新型的保护范围之内。The above descriptions are only preferred embodiments of the present utility model, and are not intended to limit the present utility model. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present utility model shall be included in the Within the protection scope of the present utility model.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720041247.7U CN206418144U (en) | 2017-01-13 | 2017-01-13 | A kind of permeability type lymphocyte blake bottle |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720041247.7U CN206418144U (en) | 2017-01-13 | 2017-01-13 | A kind of permeability type lymphocyte blake bottle |
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| CN206418144U true CN206418144U (en) | 2017-08-18 |
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| CN201720041247.7U Expired - Fee Related CN206418144U (en) | 2017-01-13 | 2017-01-13 | A kind of permeability type lymphocyte blake bottle |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109022279A (en) * | 2018-10-11 | 2018-12-18 | 广州洁特生物过滤股份有限公司 | A kind of cell-culturing rotating bottle |
| CN111117887A (en) * | 2020-01-10 | 2020-05-08 | 浙江师范大学 | Zebra fish embryo culture device |
| CN112410217A (en) * | 2020-11-06 | 2021-02-26 | 英诺维尔智能科技（苏州）有限公司 | Centrifugal culture bottle |
-
2017
- 2017-01-13 CN CN201720041247.7U patent/CN206418144U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109022279A (en) * | 2018-10-11 | 2018-12-18 | 广州洁特生物过滤股份有限公司 | A kind of cell-culturing rotating bottle |
| CN111117887A (en) * | 2020-01-10 | 2020-05-08 | 浙江师范大学 | Zebra fish embryo culture device |
| CN112410217A (en) * | 2020-11-06 | 2021-02-26 | 英诺维尔智能科技（苏州）有限公司 | Centrifugal culture bottle |
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Address after: 100070 No. 53 Building, No. 26 Waihuan West Road, Fengtai District, Beijing Patentee after: Beijing Warnoy Omei Medical Laboratory Co.,Ltd. Address before: 100070 No. 53 Building, No. 26 Waihuan West Road, Fengtai District, Beijing Patentee before: BEIJING HUANUO AOMEI MEDICAL LABORATORY CO.,LTD. |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170818 |
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