CN209010531U - A kind of microscope cell cultivation equipment - Google Patents
A kind of microscope cell cultivation equipment Download PDFInfo
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- CN209010531U CN209010531U CN201820747709.1U CN201820747709U CN209010531U CN 209010531 U CN209010531 U CN 209010531U CN 201820747709 U CN201820747709 U CN 201820747709U CN 209010531 U CN209010531 U CN 209010531U
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- cell
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- observation
- cultivation equipment
- microscope
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Abstract
The utility model discloses a kind of microscope cell cultivation equipments, comprising: cell culture chamber, cell observation cover, Tissue Culture Dish, cell gas control equipment, pedestal, side supporting surface, observation port, heating device, the first temperature sensor, second temperature sensor, air inlet, gas outlet, door, temperature controller；The utility model uses cell observation cover, and the environment of observation of constant temperature can be provided for cell culture by being provided with temperature controller, and fog is avoided to generate, and facilitates operation and observation.Using cell gas control assembly, the gas concentration in cell culture room can detecte, gas content needed for reasonable control cell culture.The design of the ware lid upper groove of culture dish simultaneously, reduces the air space above culture dish internal media, and the water mist that bottom portion of groove can be effectively prevented is formed, consequently facilitating observation.
Description
Technical field
The utility model belongs to cell cultivation equipment field more particularly to a kind of microscope cell cultivation equipment.
Background technique
Cell culture technology is also clone technology, for entire bioengineering field, cell culture be all one must
Indispensable process, cell culture technology can become simple unicellular or few differentiation by mass propgation by a cell
Many cells, or cell culture is provided and obtains a large amount of cell or its metabolite, cell culture, which refers to, to be manually provided
Under the conditions of, the growth that cell is carried out in vitro, the cell grown during the cultivation process is no longer divided into tissue, is biological skill
The basic and important means of production in art.Its purpose is to harvest cell itself or obtain products of cellular metabolism.Cell training
Supporting is one of modern biology common technology.
Cell culture is to be put into progress temperature control adjusting in temperature control device, simulation by cell culture dish holding, then by culture dish
Physiological environment, in order to cell growth.However, existing culture dish generally includes the ware body of tubular and is placed on the top surface of the ware body
It is more due to having above culture solution from culture dish is put at room temperature after removing on temperature control device for the ware lid of plane
The gas compartment forms water mist inside ware lid, is not easy to observe.Tissue Culture Dish is common vessel in cell culture, by one
The bottom of a flat circle plate-like and a lid composition, are generally made of glass or plastics.Tissue Culture Dish occupies in use
Space it is more, when cultivating cell, by ware lid gap, adjust and the gas exchanges in culture environment.Because of Tissue Culture Dish
It should not control in use, ware lid is easily slided, falls off.
In conclusion needing to design a kind of cell cultivation equipment for overcoming above-mentioned technical problem at present.
Utility model content
In view of the shortcomings of the prior art, the utility model provides a kind of microscope cell cultivation equipments, comprising: cell
Culturing room, cell observation cover, Tissue Culture Dish, cell gas control equipment, pedestal, side supporting surface, observation port, heating device,
First temperature sensor, second temperature sensor, air inlet, gas outlet, door, temperature controller.
The cell culture chamber includes pedestal and side stand component face.Wherein the side stand component face is arranged in pedestal four
Form the obturator of an all round closure in week.Cell observation cover is provided on the cell culture chamber, wherein the cell
Observation port is offered on observation cover, and the observation port is additionally provided with one for closing the shelves lid of observation port.The cell
Several gas outlets and air inlet are also provided on observation cover.Heating device, and the heating are provided in the pedestal
Device is connect with temperature controller.The top of the pedestal is inwardly recessed to form a cavity, is provided with first above the cavity
Temperature sensor, and second temperature sensor is provided in the side supporting surface.Wherein first temperature sensing
Device and the signal end of second temperature sensor are connect with temperature controller.Be additionally provided in the cell culture room Tissue Culture Dish and
Cell gas control equipment；The cell gas control equipment is through setting on the side stand component face comprising gas
Detection unit, the gas introduction unit being connect with the gas detection cell, and it is gentle with the gas detection cell
The control unit that body import unit is electrically connected.Wherein the gas detection cell includes oxygen concentration sensor and titanium dioxide
Concentration of carbon sensor.The gas introduction unit includes the gas inducting device being electrically connected with the control unit, Yi Jiyu
The object gas storage tank group of the gas inducting device connection.The control unit includes one for showing in cell culture room
Gas concentration and the operation interface that parameter is set convenient for experimenter.The Tissue Culture Dish is arranged in the cavity of pedestal,
It includes ring, wherein the ring with a thickness of 1mm, which forms the edge part of the connection culture dish of base portion
Point, the edge is on the outside of ring.Ware lid, recessed down, shape in the middle part of the ware lid are provided with above the edge
Cheng Youyi groove.Bottom film is attached at the bottom side of the ring, and the bottom film can be opened closed circular object from below
Mouthful.The inside of the ring and bottom film are formed together protrusion, and the protrusion is filled with and bonds made of silicone resin
Agent filler, to form slightly convex surface.
Be provided with control valve in the air inlet and air outlet, the control unit also with the control of air inlet/outlet
Valve is electrically connected.
The heating device is heater strip.
The bottom of the cavity is made of organic glass, forms transparent observation area.
It is additionally provided between the gas inducting device and the object gas storage tank group electrical with the gas inducting device
The gas solenoid valve of connection
The ring with a thickness of 1mm.
The ring and edge is by optically transparent material injection molding.
Protrusion is provided on the inside of the ware lid, the protrusion is in rectangular shape, offsets with edge.
The bottom film with a thickness of 100 μm.
The utility model has the advantages that
A kind of microscope cell cultivation equipment of the utility model, using cell observation cover, being provided with temperature controller can be
Cell culture provides the environment of observation of constant temperature, and fog is avoided to generate, and facilitates operation and observation.Using cell gas control group
Part can detecte the gas concentration in cell culture room, gas content needed for reasonable control cell culture.Culture dish simultaneously
Ware lid upper groove design, reduce the air space above culture dish internal media, bottom portion of groove can be effectively prevented
Water mist formed, consequently facilitating observation.It is simple and reasonable, reliable performance, low manufacture cost, practical in addition, the utility model
Property is strong, has a vast market foreground.
Detailed description of the invention:
Fig. 1 is a kind of structural schematic diagram of microscope cell cultivation equipment of the utility model；
Fig. 2 is the structural schematic diagram of the utility model Tissue Culture Dish；
Fig. 3 is the structural schematic diagram of the utility model cell gas control equipment.
Specific embodiment
Below by drawings and examples, the technical solution of the utility model is described in further detail.
It is as depicted in figs. 1 and 2 a kind of microscope cell cultivation equipment, comprising: cell culture chamber 1, cell
Observation cover 2, Tissue Culture Dish 3, cell gas control equipment 4, pedestal 5, side supporting surface 6, observation port 7, heating device 9, first
Temperature sensor 10, second temperature sensor 11, air inlet 12, gas outlet 13, door 14, temperature controller 15.
The cell culture chamber 1 includes pedestal 5 and side stand component face 6.Wherein the side stand component face 6 is arranged the bottom of at
5 surroundings of seat, form the obturator of an all round closure.It is provided with cell observation cover 2 on the cell culture chamber 1, wherein institute
Observation port 7 is offered on the cell observation cover 2 stated, and the observation port 7 is additionally provided with one for closing the shelves lid of observation port 7
14.Several gas outlets 13 and air inlet 12 are also provided on the cell observation cover 2.Heating is provided in the pedestal 5
Device 9, wherein the heating device 9 is heater strip, and the heating device 9 is connect with temperature controller 15.The bottom
The top of seat 5 is inwardly recessed to form a cavity, and the bottom of the cavity is made of organic glass, forms transparent observation area.Institute
It is provided with the first temperature sensor 10 above the cavity stated, and is provided with second temperature sensing in the side supporting surface 6
Device 11.Wherein first temperature sensor 10 and the signal end of second temperature sensor 11 are connect with temperature controller 15.It is logical
The temperature that temperature controller 15 controls the heating device 9 is crossed, to control the growing environment of culture solution, and passes through temperature sensing
The temperature that device will test is transmitted back to temperature controller by signal.Tissue Culture Dish 3 and cell are additionally provided in the cell culture chamber 1
Gas control equipment 4；The cell gas control equipment 4 is through setting on the side stand component face 6 comprising gas inspection
Survey unit 24, the gas introduction unit 25 that connect with the gas detection cell 24, and with the gas detection cell
24 and gas introduction unit 25 be electrically connected control unit 27.Wherein the gas detection cell 24 is passed including oxygen concentration
Sensor 28 and gas concentration lwevel sensor 29.It is detected by oxygen concentration sensor 28 and gas concentration lwevel sensor 29
Gas concentration information is passed to control unit 27 by oxygen and gas concentration lwevel in cell culture chamber 1.The gas imports
Unit 25 includes the gas inducting device being electrically connected with the control unit 27, and the mesh connecting with the gas inducting device
Mark gas storage tanks group 26.When control unit 27 judges that oxygen concentration is inadequate or gas concentration lwevel is inadequate, control is single
Member 27 opens gas inducting device and corresponding gas storage tanks, is filled with gas into cell culture chamber 1.The control unit 27
Including one for showing gas concentration in cell culture chamber 1 and the operation interface of parameter being arranged convenient for experimenter.The gas
The gas electromagnetism being electrically connected with the gas inducting device is additionally provided between body inducting device and the object gas storage tank group 26
Valve.By the opening and closing of solenoid valve, the opening and closing of gas storage tanks is controlled.In addition, the control unit 27 also with air inlet/outlet
Control valve 16 is electrically connected, and the air intake-exhaust of air inlet and air outlet is controlled by control unit.
The Tissue Culture Dish 3 is arranged in the cavity of pedestal 5 comprising ring 32, wherein the ring
32 with a thickness of 1mm, which forms 33 part of edge of the connection culture dish of base portion, and the edge 33 is in ring
32 outsides.The ring 32 and edge 33 is by optically transparent material injection molding.Ware is provided with above the edge 33
Lid 34 is provided with protrusion 35 on the inside of the ware lid 34, and protrusions 35 are in rectangular shape, the protrusion 35 and 33 phase of edge
It supports.34 middle part of ware lid is recessed down, is formed with a groove 36, the air reduced above culture dish internal media is empty
Between, the water mist that 36 bottom of groove can be effectively prevented is formed, consequently facilitating observation.Bottom is attached at the bottom side of the ring 32
Portion's film 37, the thickness of the bottom film 37 are less than the thickness of bottom annular object 32, and the bottom film 37 can closed-loop from below
Shape object opening.Wherein the bottom membrane material is polyethylene-polypropylene composite material, bottom film 37 with a thickness of 100 μm.Institute
The inside 38 for the ring 32 stated and bottom film 37 are formed together protrusion, and the protrusion is filled with and bonds made of silicone resin
Agent filler 39, to form slightly convex surface 40.
In conclusion the content of the utility model is not limited in the above embodiments, those skilled in the art can
To propose other embodiments within technological guidance's thought of the invention, but these embodiments are included in the scope of the present invention
Within.
Claims (9)
1. a kind of microscope cell cultivation equipment characterized by comprising cell culture chamber, cell observation cover, cell culture
Ware, cell gas control equipment, pedestal, side supporting surface, observation port, heating device, the first temperature sensor, second temperature sensing
Device, air inlet, gas outlet, door, temperature controller；
The cell culture chamber includes pedestal and side stand component face；Wherein the side stand component face is arranged in pedestal surrounding,
Form the obturator of an all round closure；Cell observation cover is provided on the cell culture chamber, wherein the cell is seen
It examines and offers observation port on cover, and the observation port is additionally provided with one for closing the shelves lid of observation port；The cell is seen
It examines and is also provided with several gas outlets and air inlet on cover；Heating device, and the heating dress are provided in the pedestal
It sets and is connect with temperature controller；The top of the pedestal is inwardly recessed to form a cavity, and the first temperature is provided with above the cavity
Sensor is spent, and is provided with second temperature sensor in the side supporting surface；Wherein first temperature sensor
It is connect with temperature controller with the signal end of second temperature sensor；Tissue Culture Dish and thin is additionally provided in the cell culture room
Born of the same parents' gas control equipment；The cell gas control equipment is through setting on the side stand component face comprising gas inspection
Survey unit, the gas introduction unit that connect with the gas detection cell, and with the gas detection cell and gas
The control unit that import unit is electrically connected；Wherein the gas detection cell includes oxygen concentration sensor and carbon dioxide
Concentration sensor；The gas introduction unit includes the gas inducting device being electrically connected with the control unit, and with institute
State the object gas storage tank group of gas inducting device connection；The control unit includes one for showing gas in cell culture room
Bulk concentration and the operation interface that parameter is set convenient for experimenter；The Tissue Culture Dish is arranged in the cavity of pedestal,
Including ring, wherein the ring with a thickness of 1mm, which forms the edge part of the connection culture dish of base portion
Point, the edge is on the outside of ring；Ware lid, recessed down, shape in the middle part of the ware lid are provided with above the edge
Cheng Youyi groove；Bottom film is attached at the bottom side of the ring, and the bottom film can be opened closed circular object from below
Mouthful；The inside of the ring and bottom film are formed together protrusion, and the protrusion is filled with and bonds made of silicone resin
Agent filler, to form slightly convex surface.
2. a kind of microscope cell cultivation equipment according to claim 1, it is characterised in that: the air inlet and go out
Control valve is provided in port, the control unit is also electrically connected with the control valve of air inlet/outlet.
3. a kind of microscope cell cultivation equipment according to claim 1, it is characterised in that: the heating device is
Heater strip.
4. a kind of microscope cell cultivation equipment according to claim 1, it is characterised in that: the bottom of the cavity
It is made of organic glass, forms transparent observation area.
5. a kind of microscope cell cultivation equipment according to claim 1, it is characterised in that: the gas inducting device
The gas solenoid valve being electrically connected with the gas inducting device is additionally provided between the object gas storage tank group.
6. a kind of microscope cell cultivation equipment according to claim 1, it is characterised in that: the thickness of the ring
Degree is 1mm.
7. a kind of microscope cell cultivation equipment according to claim 1, it is characterised in that: the ring and side
Cause optically transparent material injection molding.
8. a kind of microscope cell cultivation equipment according to claim 1, it is characterised in that: set on the inside of the ware lid
It is equipped with protrusion, the protrusion is in rectangular shape, offsets with edge.
9. a kind of microscope cell cultivation equipment according to claim 1, it is characterised in that: the thickness of the bottom film
Degree is 100 μm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820747709.1U CN209010531U (en) | 2018-05-19 | 2018-05-19 | A kind of microscope cell cultivation equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820747709.1U CN209010531U (en) | 2018-05-19 | 2018-05-19 | A kind of microscope cell cultivation equipment |
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| Publication Number | Publication Date |
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| CN209010531U true CN209010531U (en) | 2019-06-21 |
Family
ID=66828340
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820747709.1U Active CN209010531U (en) | 2018-05-19 | 2018-05-19 | A kind of microscope cell cultivation equipment |
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| CN (1) | CN209010531U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112481129A (en) * | 2021-01-15 | 2021-03-12 | 北京图腾猎技科技有限公司 | Cell culture instrument for immune cell amplification |
| WO2022087862A1 (en) * | 2020-10-28 | 2022-05-05 | 陆倩雯 | Multifunctional carbon dioxide petri dish |
-
2018
- 2018-05-19 CN CN201820747709.1U patent/CN209010531U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022087862A1 (en) * | 2020-10-28 | 2022-05-05 | 陆倩雯 | Multifunctional carbon dioxide petri dish |
| CN112481129A (en) * | 2021-01-15 | 2021-03-12 | 北京图腾猎技科技有限公司 | Cell culture instrument for immune cell amplification |
| CN112481129B (en) * | 2021-01-15 | 2021-07-09 | 北京达济康华生物科技有限责任公司 | Cell culture instrument for immune cell amplification |
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