CN209352926U - A new type of easy-to-use cell isolation culture box - Google Patents

Abstract

The utility model discloses a novel and easy-to-use cell isolation culture box, which comprises a box body, a storage cavity is opened inside the box body, grooves are opened on four sides of the box body, and grooves of the groove A socket is opened through the middle part, a filter is clamped in the groove, and a filter hole is opened on the filter. This new type of easy-to-use cell isolation culture box has a simple structure, low price, and strong versatility. It not only solves the problem of cross-contamination in the incubator, but also avoids direct air contact between the cells and the environment during the transfer process, greatly reducing the number of cells. During the cultivation process, the probability of biological pollution; it can not only ensure that the carbon dioxide concentration and humidity of the internal environment are the same as the outside world, but also isolate the entry and exit of common bacteria and reduce cell contamination; the filter used is a routine consumable in the laboratory and can be replaced; isolate cells The culture box has a simple structure, can be repeatedly sterilized and reused, and saves resources.

Description

A new type of easy-to-use cell isolation culture box

technical field

The utility model relates to the technical field of culture boxes, in particular to a novel and easy-to-use cell isolation culture box.

Background technique

Cell culture is a commonly used research method in medicine and biology. Pollution during the culture process is inevitable. Pollution can be divided into three categories: physical, chemical, and biological pollution. Among them, biological pollution is common and the consequences are relatively serious. Once the cultured cells are contaminated by microorganisms, they will be irreversible. In the lightest cases, the polluted cells will be discarded, and in severe cases, other cells in the incubator or even the entire laboratory will be polluted. The environment has brought serious losses and threats to scientific research. The commonly used cell incubator monitors the carbon dioxide concentration and humidity as a whole. Each culture bottle or dish communicates with each other through the air in the incubator. Once one of them is contaminated by microorganisms, it is likely to cause cell contamination in the entire incubator. In addition, we take the cells out of the incubator and operate them in a biological safety cabinet. During this process, the culture dish or culture bottle is communicated with the surrounding air, and there is also the risk of being contaminated by microorganisms in the outside air. If there is a container that can provide an air-permeable but impermeable microorganism-proof isolation culture environment, and has no influence on the existing mature cell culture technology, it can significantly reduce cell contamination and be convenient and easy to use. Traditional partitioned incubators are expensive, low in popularity, and have a fixed space.

Utility model content

(1) Solved technical problems

Aiming at the deficiencies of the prior art, the utility model provides a novel and easy-to-use cell isolation culture box, which solves the troublesome problem of using the existing cell culture box.

(2) Technical solutions

In order to achieve the above purpose, the utility model provides the following technical solutions: a novel and easy-to-use cell isolation culture box, including a box body, the inside of the box body is provided with a storage cavity, and the four sides of the box body are provided with A groove, the middle part of the groove is opened with a socket, and a filter is clamped in the groove, and a filter hole is opened on the filter, and one side of the filter passes through the socket and extends into the storage cavity , a culture dish and a culture plate are respectively placed in the storage cavity, a culture tank is provided on the top of the culture plate, a buckle convex ridge is arranged on the top of the box body, and the top of the buckle convex ridge is fastened with a lid.

Preferably, a sealing plastic ring is bonded to the inner edge of the box cover.

Preferably, the bottom of the box is provided with a trapezoidal opening, and heat insulation pads are attached to the left and right sides of the bottom of the box.

Preferably, the number of the culture dishes is 5-8, the number of the culture plates is 2-3, and the culture dishes and the culture plates are stacked on each other in a stacking manner.

Preferably, the filter holes have a pore size of 0.2-0.22 microns.

(3) Beneficial effects

The utility model provides a novel and easy-to-use cell isolation culture box. Has the following beneficial effects:

This new type of easy-to-use cell isolation culture box has a simple structure, low price, and strong versatility. It not only solves the problem of cross-contamination in the incubator, but also avoids direct air contact between the cells and the environment during the transfer process, greatly reducing the number of cells. During the cultivation process, the probability of biological pollution; it can not only ensure that the carbon dioxide concentration and humidity of the internal environment are the same as the outside world, but also isolate the entry and exit of common bacteria and reduce cell contamination; the filter used is a routine consumable in the laboratory and can be replaced; isolate cells The culture box has a simple structure, can be sterilized repeatedly, and saves resources; and the box body is small, and can be applied to the original incubators of different sizes without additional high investment, which is extremely versatile and popular.

Description of drawings

Fig. 1 is a front view of the structure of the utility model;

Fig. 2 is a front sectional view of the utility model box structure;

Fig. 3 is a top sectional view of the utility model box structure;

Fig. 4 is a bottom view of the lid structure of the utility model.

In the figure: 1, box body; 101, storage cavity; 2, groove; 201, socket; 3, filter; 4, filter hole; 5, culture dish; 6, culture plate; 7, culture tank; 8, buckle 9. Box cover; 10. Sealing plastic ring; 11. Trapezoidal opening; 12. Heat insulation pad.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. example. Based on the embodiments of the present utility model, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the scope of protection of the present utility model.

As shown in Figures 1-4, the utility model provides a technical solution: a novel and easy-to-use cell isolation culture box, including a box body 1, a storage chamber 101 is opened inside the box body 1, and four compartments of the box body 1 Both sides are provided with a groove 2, and the middle part of the groove 2 is provided with a socket 201, and a filter 3 is clamped in the groove 2, and a filter hole 4 is opened on the filter 3, and the diameter of the filter hole 4 is 0.2-0.22 microns One side of the filter 3 runs through the socket 201 and extends into the storage chamber 101. The box body 1 and the box cover 9 are rectangular, and the polypropylene material that can withstand autoclaving is used, and there are different sizes according to different cell culture dishes 5. The size of the box body 1, there are grooves 2 on the four sides of the box body 1, and the sockets 201 of the external filter 3 are respectively provided in the center of the grooves 2, and the external filter 3 is connected to the socket 201, and the filter 3 is commonly used in the laboratory. The filter can be replaced, so as to achieve the purpose of air permeability but no bacteria. The culture dish 5 and the culture plate 6 are respectively placed in the storage chamber 101. The number of the culture dish 5 is 5-8, and the number of the culture plate 6 is 2-3. Each, the culture dish 5 and the culture plate 6 are stacked on each other in a stacking manner, the top of the culture plate 6 is provided with a culture tank 7, the top of the box body 1 is provided with a buckle convex ridge 8, and the top buckle of the buckle convex ridge 8 It is combined with a box cover 9, and the height of the buckled convex ridge 8 is 1 mm, which is convenient to fasten with the box cover 9, so that the isolation culture box can be stacked stably, has a simple structure, can be used repeatedly, and is extremely easy to use and popularize. The inner edge of the box cover 9 is fitted with a sealing plastic ring 10, the bottom of the box body 1 is provided with a trapezoidal opening 11, and the left and right sides of the bottom of the box body 1 are fitted with heat insulating pads 12, and the height of the trapezoidal opening 11 is 2 cm. , so that the bottom surface is suspended, when there are cells in the box, even if the isolation culture box is placed on a table with a large temperature difference, the cells in it will not have excessive temperature changes in a short period of time.

During use, the box body 1 and the box cover 9 are rectangular, and the polypropylene material that can withstand autoclaving is used. There are different box body 1 sizes according to different cell culture dishes 5 sizes, and four sides of the box body 1 are respectively provided with Groove 2, the middle position of groove 2 is respectively provided with the socket 201 of external filter 3, the socket 201 is connected with filter 3 inside and outside, filter 3 is the filter commonly used in the laboratory, can be replaced, thereby achieves the purpose of breathable but impervious to bacteria, The height of the trapezoidal opening 11 is 2 cm, so that the bottom surface is suspended. When there are cells in the box, even if the isolation culture box is placed on a table with a large temperature difference, the cells in it will not have excessive temperature changes in a short period of time. The height of the raised ridge 8 is 1 mm, which is convenient to fasten with the lid 9, so that the isolation culture box can be stacked firmly, has a simple structure, can be used repeatedly, and is extremely easy to use and popularize.

To sum up, this new type of easy-to-use cell isolation culture box has a simple structure, low price, and strong versatility. It not only solves the problem of cross-contamination in the incubator, but also avoids direct air contact between the cells and the environment during the transfer process. Contact greatly reduces the probability of biological contamination during the cell culture process; it can not only ensure that the carbon dioxide concentration and humidity of the internal environment are the same as the outside world, but also isolate the entry and exit of common bacteria and reduce cell contamination; the filter 3 used is a routine laboratory consumable , can be replaced; the isolated cell culture box has a simple structure, can be repeatedly sterilized and reused, and saves resources; and the box body is small, and it can be applied to the original incubators of different sizes without additional high investment, which is extremely versatile and popular powerful.

It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply that there is a relationship between these entities or operations. any such actual relationship or order exists between them. Furthermore, the term "comprises", "comprises" or any other variation thereof is intended to cover a non-exclusive inclusion such that a process, method, article or apparatus comprising a set of elements includes not only those elements, but also includes elements not expressly listed. other elements of or also include elements inherent in such a process, method, article, or apparatus.

Although the embodiments of the present invention have been shown and described, those skilled in the art can understand that various changes and modifications can be made to these embodiments without departing from the principle and spirit of the present invention , replacements and modifications, the scope of the present utility model is defined by the appended claims and their equivalents.

Claims (5)

1. a kind of novel simple and easy-to-use cell isolation culture box, including box body (1), the inside of the box body (1), which offers, is set Object chamber (101), it is characterised in that: four sides of the box body (1) open up fluted (2), and the middle part of the groove (2), which is run through, opens Equipped with socket (201), it is connected with filter (3) in the groove (2), is offered filter opening (4) on the filter (3), it is described The side of filter (3) is through socket (201) and extends in object placing cavity (101), is placed with respectively in the object placing cavity (101) Culture dish (5) and culture plate (6) are offered culture tank (7) at the top of the culture plate (6), are set at the top of the box body (1) It is equipped with the convex bank of snapping (8), the top of the convex bank of snapping (8), which fastens, there are box cover (9).

2. the novel simple and easy-to-use cell isolation culture box of one kind according to claim 1, it is characterised in that: the box cover (9) edge on the inside of is fitted with sealed plasthetic circle (10).

3. the novel simple and easy-to-use cell isolation culture box of one kind according to claim 1, it is characterised in that: the box body (1) bottom offers trapezoidal mouth (11), and the left and right sides of box body (1) bottom is fitted with heat insulating mattress (12).

4. the novel simple and easy-to-use cell isolation culture box of one kind according to claim 1, it is characterised in that: the culture The quantity of ware (5) is 5-8, and the quantity of the culture plate (6) is 2-3, and the culture dish (5) and culture plate (6) are adopted It is stacked with the mode of stacking.

5. the novel simple and easy-to-use cell isolation culture box of one kind according to claim 1, it is characterised in that: the filter opening (4) aperture is 0.2-0.22 microns.