KR200245301Y1 - The culturing apparatus for panax ginseng roots - Google Patents

Abstract

The present invention relates to a culture root (Roots) incubator of Panax ginseng CA Meyer, the production cost is much lower than the conventional incubator, light and shock-resistant, easy to manage, but also moist heat sterilization, cultivation of ginseng culture root An efficient incubator is provided.

In addition, the incubator of the present invention can be usefully applied to plant cell culture such as tobacco, carrot, and the like, which are cultured by air flotation apparatus without stirring the culture.

Description

Ginseng culture root incubator {THE CULTURING APPARATUS FOR PANAX GINSENG ROOTS}

The present invention relates to a culture root incubator of Panax ginseng C.A.Meyer.

Ginseng is a perennial herb of the Araliaceae , which sprouts in March and blooms in mid-May. It grows up to 40 ~ 60cm.

Ginseng is very difficult to grow, so it is difficult to grow on farms. In recent years, studies have been conducted to produce large amounts of cells, roots and hairy roots using tissue culture methods. Especially, tissue culture methods can produce large amounts of raw ginseng regardless of the season within a short period of time. It can produce unprocessed pollution-free ginseng.

Known techniques related to tissue culture of ginseng include the method of producing somatic embryos and seedlings by suspension culture of embryogenic cells of ginseng and its use (Korean patent application 10-1999-0013865), ginseng cells in growth-free culture vessel Although there are a number of cultivation methods, such as mass production of larvae and production methods of seedlings (Korean Patent Application No. 10-1999-005865), little is explained about the incubator.

On the other hand, in the balloon-type air-reaction bioreactor for cell culture (Korean Patent Application No. 10-1997-035751), there is related to the incubator for culturing cells, such as yew, ginseng, tobacco, carrot, etc., the incubator of more than 100L scale body material This stainless steel does not provide light, so it is difficult to apply to ginseng hair root cultivation.

Conventionally, 20 l incubators, such as those used in the cultivation of carrot or tobacco cells, can provide light because the body material is made of glass, but the manufacturing cost is high, and it is difficult to carry and manage due to being fragile and heavy.

The present invention to solve the above problems, the production cost is cheaper than the conventional incubator, light and impact resistant to easy to manage and moist heat sterilization to provide an efficient incubator for ginseng culture root culture.

1 is a conventional incubator

Figure 2 is a perspective view of the ginseng culture root incubator of the present invention

3 is a detailed structure of the air injection unit

Figure 4 is an exploded perspective view of the air injection parts

5 is a detailed structure of the air discharge unit

6 is an exploded perspective view of the air exhaust parts

7 is a culture example of ginseng cultured root using the incubator of the present invention

* Explanation of symbols for main parts of the drawings

10 body 13 air filter

20: body 21: neck

22: plug 23: air purification filter

24: air volume regulator 27: rubber tube

29: aluminum foil 30: air injection unit

31: O-ring 32: Silicone member

33: funnel-shaped glass tube 34: sparger

50: air outlet 51: stainless steel pipe

52 silicon member 64 cotton

71: air bubble 72: ginseng culture root

73: pedestal 74: air purification filter

75: compressor

Ginseng culture root incubator of the present invention is composed of a cylindrical body 20 made of a transparent plastic material, the air injection portion 30, the air discharge portion 50 and the connecting parts.

Hereinafter, a detailed description will be given with reference to FIGS. 2 to 6.

Cylindrical body 20 is made of a plastic, such as polycarbonate (Polycarbonote) having a transparent and impact resistance like the material of the normal bottled water, the shape of the body is similar to the bottle of water of 18ℓ size, but the neck portion (21) ) Is larger than the diameter of the neck of the bottle, and after several experiments, it is about half the diameter of the body to be suitable for the liquid floating culture, and the other side forms a flat surface. It can be erected without a support device for easy storage.

At the end of the neck portion 21 of the air inlet 30, there is a turning and closing plastic stopper 22, which opens and closes the stopper so that the medium and hairy roots can be put or harvested, and a circular sparger in the middle of this stopper ( The funnel-shaped glass tube 33, which is attached to 34, is fixed by the silicone member 32 while being pushed outwards from the inside of the stopper, and has an O-ring 31 at the inner end of the stopper to close the container by turning the stopper. It is firmly attached to the body of the to prevent leakage of the culture medium.

There are various forms of sparger such as cross, rhombus, and bilateral. After several experiments, circular sparger was most suitable for liquid floating culture of hairy root.

The funnel-shaped glass tube 33 of the air inlet is connected to the air purifying filter 23 and the air volume regulator 24 through the rubber tube 27 so as to control the amount of air supplied or to block the supply of unnecessary air when not in use. It is.

On the other hand, the structure of the air discharge portion 50 on the side of the upper side of the body is fixed while drilling a hole in the body and pushing the silicon member 52, the stainless steel tube 51 is inserted from the inside of the body of the culture vessel to the outside This prevents the air discharge part 50 from escaping from the water pressure or the air pressure.

The stainless steel tube is connected to a rubber tube 27 containing a cotton 64 for pollution prevention, and the end is covered with an aluminum foil 29 for pollution prevention.

The incubator of the present invention having the above-described structure is very efficient for culturing ginseng hairy roots and is also useful for culturing plant cells such as tobacco and carrots grown by air flotation without stirring.

The culture example of the ginseng culture root using the incubator of the present invention will be described.

EXAMPLES Cultivation of Ginseng Cultured Roots Using the Incubator of the Present Invention

(1) After the incubator body was turned upside down with a capacity of 20ℓ, the stopper was opened and 50 g of culture medium and ginseng culture root were added.

(2) After turning the stopper closed, the neck of the incubator was placed upright on the pedestal, and then the air injection tube was connected.

(3) An aluminum foil was wrapped around the end of the air discharge tube.

(4) The air volume regulator was opened to supply fine air bubbles through the sparger.

(5) Incubated for 45 days while maintaining the proper temperature while supplying light and air.

(6) About 2 kg of ginseng cultured root of good quality was harvested.

According to the present invention, the production cost is much lower than that of the conventional incubator, and the light and impact resistant is easy to manage, but also provides an efficient incubator for cultivating ginseng cultured roots.

Claims (2)

In the ginseng culture root incubator composed of a body, an air inlet, an air outlet, and a connecting part, the cylindrical body 20 is made of a transparent plastic such as polycarbonate, and the diameter of the lower neck 21 is the diameter of the body. It is about half the size, and there is an air injection unit 30 at the end of the neck, and the glass tube of the air injection unit is connected to the air purification filter 23 and the air volume regulator 24 through the rubber tube 27, and the upper part of the body 20. The air outlet part 50 is formed at the side circumference, and the cotton 54 is filled in the inner end of the rubber tube 27 connected to the stainless steel tube of the air outlet part, and the outer end of the rubber tube is covered with an aluminum foil 29. Ginseng culture root incubator is characterized in that. According to claim 1, the structure of the air injection portion 30, the silicon member 32 while pushing the funnel-shaped glass tube 33 is attached to the central portion of the stopper 21 with a spherical sparger 34 from the inside of the stopper to the outside. Ginseng culture root incubator characterized in that the structure is fixed using.