CN108522247A - A kind of bioreactor of culture ginseng adventitious root - Google Patents
A kind of bioreactor of culture ginseng adventitious root Download PDFInfo
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- CN108522247A CN108522247A CN201810398406.8A CN201810398406A CN108522247A CN 108522247 A CN108522247 A CN 108522247A CN 201810398406 A CN201810398406 A CN 201810398406A CN 108522247 A CN108522247 A CN 108522247A
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- tank body
- ontology
- bioreactor
- disk
- fixing piece
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- 241000208340 Araliaceae Species 0.000 title abstract description 19
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 title abstract description 19
- 235000003140 Panax quinquefolius Nutrition 0.000 title abstract description 19
- 235000008434 ginseng Nutrition 0.000 title abstract description 19
- 239000002184 metal Substances 0.000 claims abstract description 13
- 229910052751 metal Inorganic materials 0.000 claims abstract description 13
- 238000005245 sintering Methods 0.000 claims abstract description 12
- 238000011081 inoculation Methods 0.000 claims description 7
- 238000012546 transfer Methods 0.000 claims description 6
- 239000007769 metal material Substances 0.000 claims description 5
- 125000004122 cyclic group Chemical group 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 10
- 230000008569 process Effects 0.000 abstract description 7
- 238000009395 breeding Methods 0.000 abstract description 6
- 230000001488 breeding effect Effects 0.000 abstract description 6
- 238000012797 qualification Methods 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 238000000855 fermentation Methods 0.000 abstract description 2
- 230000004151 fermentation Effects 0.000 abstract description 2
- 239000007789 gas Substances 0.000 description 15
- 230000000694 effects Effects 0.000 description 6
- 238000001914 filtration Methods 0.000 description 5
- 239000010410 layer Substances 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 238000003466 welding Methods 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 229910001315 Tool steel Inorganic materials 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 229910000838 Al alloy Inorganic materials 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 230000003749 cleanliness Effects 0.000 description 1
- 230000001427 coherent effect Effects 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 208000002925 dental caries Diseases 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000003475 lamination Methods 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000011241 protective layer Substances 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 230000021749 root development Effects 0.000 description 1
- 230000002786 root growth Effects 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention provides a kind of bioreactors, it is cultivated for the adventitious root to biology, the reactor includes tank body, the bottom of the tank body is provided with air sparger, the air sparger includes the ontology snapped together and disk, the ontology includes the interface structure being connected to reactor outside, and the gas outside the reactor passes sequentially through the interface structure, the disk enters in the tank body, and the disk includes at least metal sintering stratum reticulare.Bioreactor provided by the invention can realize that large-scale breeding is cultivated to ginseng adventitious root, to produce the high industrialization ginseng adventitious root product of stable quality, qualification rate.Bioreactor in the present invention only needs to carry out centainly being transformed on general fermentation tank to can be used to ginseng adventitious root breeding, and reactor low cost is easy to get, and reduces the production cost in ginseng adventitious root cultivating process, and large-scale promotion is suitble to use.
Description
Technical field
The present invention relates to biological breeding apparatus fields, and in particular to a kind of bioreactor.
Background technology
With the development of society and the improvement of people's living standards, people are higher and higher to the attention degree of health.People
Ginseng is increasingly pursued as a kind of holy product of nourishing.The training method of ginseng is carried out with culturing and transplanting seedlings or direct-seeding mostly,
The speed of growth is slow, cannot be satisfied people to ginseng and the ever-increasing demand of ginseng adventitious root.
Laboratory stage is substantially in the culture of ginseng adventitious root in the prior art, in the mistake cultivated it
In journey using to reactor be laboratory scale small-sized biological reactor, use reactor carry out single culture reaction
Body product is between 5-20 liters.These experiments are mostly glass gas lift tank or PC gas lift buckets with small-sized biological reactor, not only body
Product is small, and equipment is simple, cannot achieve and efficiently cultivates ginseng adventitious root.
Invention content
It is an object of the invention to for the problem that ginseng adventitious root breeding amount is small, reproductive efficiency is low in the prior art, carry
For the bioreactor that a kind of culture scale is big, culture efficiency is high, the adventitious root qualification rate of culture is high.
In order to solve the above technical problems, the present invention adopts the following technical scheme that：
A kind of bioreactor is cultivated for the adventitious root to biology, and the reactor includes tank body, the tank body
Bottom be provided with air sparger, the air sparger includes the ontology snapped together and disk, and the ontology includes
With the interface structure that is connected to outside the reactor, the gas outside the reactor passes sequentially through the interface structure, described
Disk enters in the tank body, and the disk includes at least metal sintering stratum reticulare.
Preferably, the ontology includes the middle part curved-surface structure that recess is formed downwards, between the ontology and the disk
Cavity is formed, the ontology is formed by fixed structure with the disk and is removably fixedly connected, and/or,
The ontology is made of metal material.
Preferably, the fixed structure include the first fixing piece and the second fixing piece, first fixing piece with described
Body is connected and is located at the side far from the disk on the ontology, and second fixing piece is connected with the disk and position
In the side far from the ontology in the disk, first fixing piece and second fixing piece are fixed on by fastener
Together, the ontology and the disk are clamped between first fixing piece and second fixing piece.
Preferably, first fixing piece and the second fixing piece structure annular in shape, first fixing piece and described
Second fixing piece is located at the edge of the ontology and the disk.
Preferably, the bioreactor further includes agitating device, and the agitating device includes by the top of the tank body
The column structure extended to the bottom of the tank body,
On the column structure propeller blade is arranged at interval of predetermined length.
Preferably, one end of the bottom on the column structure positioned at the tank body is provided with propeller type shear-blade.
Preferably, the agitating device further includes power part, and institute is located on the power part and the column structure
The one end for stating top of the tank is fixedly connected, and the power part drives the column structure rotation and/or swing, with to the tank
Internal adventitious root is disturbed.
Preferably, the wall structure of the bottom of the tank body is in back taper, and the air sparger is arranged in the back taper
The bottom end of shape, the outside that the interface structure extend out to the tank body are connected with vent line.
Preferably, it is provided with transfer outlet on the wall structure of the bottom of the tank body, the transfer is exported for by position
Adventitious root in the bottom of the tank body is removed from the tank body, and/or,
Inoculation entrance is provided on the wall structure at the top of the tank body, the inoculation entrance is used for into the tank body
It is inoculated with the adventitious root.
Preferably, outlet passageway structure, the outlet passageway structure are provided on the wall structure at the top of the tank body
Including vertically extending condenser pipe.
Bioreactor provided by the invention can realize that large-scale breeding is cultivated to ginseng adventitious root, to produce mass
Stablize, the industrialization ginseng adventitious root product that qualification rate is high.
Bioreactor in the present invention only needs to carry out centainly being transformed on general fermentation tank to can be used to ginseng not
Determine root propagation, reactor low cost is easy to get, and reduces the production cost in ginseng adventitious root cultivating process, is suitble to big rule
Mould is promoted the use of.
Description of the drawings
Fig. 1 show the overall structure diagram for the bioreactor that the embodiment of the present invention 1 provides；
Fig. 2 show the mounting structure schematic diagram for the fixed structure that the embodiment of the present invention 1 provides.
Wherein,
1. tank body, 11. tank ontologies, 12. wall structures, 13. inoculation entrances,
2. air sparger, 21. ontologies, 211. interface structures, 22. disks, 23. cavitys,
3. holder,
4. vent line,
5. fixed structure, 51. first fixing pieces, 52. second fixing pieces,
6. agitating device, 61. column structures, 62. power parts, 63. propeller blades, 64. propeller types are cut
Cutting edge, 641. shear blades,
7. outlet passageway structure, 71. condenser pipes.
Specific implementation mode
Bioreactor provided by the invention is described in more detail below in conjunction with the accompanying drawings.
Explanation：It is upper and lower on the basis of direction shown in FIG. 1 during present invention description.
Embodiment 1
As depicted in figs. 1 and 2, a kind of bioreactor is present embodiments provided, is trained for the adventitious root to biology
Support, for example, ginseng adventitious root.The reactor includes tank body 1, and the tank body 1 includes tank ontology 11, and the tank body 1 is by metal
Material is made, it is preferred to use high temperature resistant, corrosion resistant metal material, such as aluminium alloy or high-speed tool steel.The bottom of the tank body 1
The wall structure 12 in portion is in back taper, in the tank body 1 of back taper bottom wall structure 12 and the tank ontology 11 it
Between can be structure as a whole or separate structure, when it is both above-mentioned be separate structure when, it is preferred to use welding manner is by two
Person is fixed together.Be additionally provided with holder 3 in the bottom of the tank body 1, to be supported to the tank body 1, the holder 3 with
It is preferably fixed together using welding manner between the tank body 1, to enhance intensity between the two, is prevented in the tank body 1
Weight is larger after culture solution and adventitious root is added, and the holder 3 due to load-bearing because that the problem of damage can not occur.
Further, the bottom of the tank body 1 is provided with air sparger 2, in the present embodiment, the air sparger
2 are arranged in the bottom end of the back taper.It is described since the adventitious root in the tank body 1 is gradually grown up in incubation
The weight of adventitious root is also to gradually increase, and therefore, the adventitious root can decline in its own gravity.By the tank body 1
The wall structure 12 of bottom be set as back taper, can allow the overall compact bottom for concentrating on back taper wall structure 12 of adventitious root
Portion.Simultaneously as the air sparger 2 is arranged in the bottom end of the back taper so that the air sparger 2 was produced by boasting
The adventitious root for being deposited in 1 bottom of the tank body can preferably be blowed to the middle and upper part of the tank body 1 by bubble again, and it is indefinite to ensure
Root disperses more uniformly in the tank body 1, to ensure that each adventitious root can be absorbed into equilibrium in the tank body 1
Nutrition, and then ensure to cultivate reliability, improve the cultivation qualification rate of adventitious root.
The air sparger 2 includes the ontology 21 that snaps together and disk 22, the ontology 21 include with it is described anti-
The interface structure 211 being connected to outside device is answered, the outside that the interface structure 211 extend out to the tank body 1 is connected with vent line 4
It connects, clean air can be passed through in the vent line 4, or other gas needed in adventitious root incubation
Body.Control valve (not shown), the control of the control valve and the bioreactor are provided in the interface structure 211
Device (not shown) processed is connected, and the control device can control the opening and closing of the control valve to control the interface knot
The break-make of air-flow in structure 211.Gas outside the reactor passes sequentially through the interface structure 211, the disk 22 enters
Into the tank body 1, the disk 22 includes at least metal sintering stratum reticulare.The metal sintering stratum reticulare uses the wire of multilayer
It is combined by techniques such as special lamination compacting, vacuum-sinterings and to be fabricated.The metal sintering stratum reticulare includes five layers, mistake
Filtering layer, protective layer, supporting course, separating layer and enhancement layer, the metal in the present embodiment for manufacturing the metal sintering stratum reticulare
Material is preferably high-speed tool steel.The existing uniform and stable filtering accuracy of metal sintering stratum reticulare have again higher intensity with just
Degree, while also there is higher compression strength and grade of filtration.The metal sintering stratum reticulare is surface layer mistake due to its work principle of filter
Filter, and it is smooth by the mesh duct that wire is formed, therefore it can make for a long time repeatedly with excellent backwash and regeneration performance
With, can be used the methods of counter-flow water, filtrate, ultrasonic wave, melting, baking cleaning it is cleaned.Meanwhile forming the metal
The metal sintering net materials of sintering stratum reticulare are easy to be molded, and can be processed and weld operation to it, can the system of easily processing
It is made the various forms of filter elements such as circle, tubular, taper, corrugated.The sintered metal mesh has wide range of applications, energy
The temperature environment and acid or alkali environment for being enough used in -200 DEG C~600 DEG C are filtered, be particularly suitable for serialization with it is automatic
The operating process of change, which is any type filtering material, can not all compare.
Further, the ontology 21 of the air sparger 2 includes that middle part is recessed downwards the curved-surface structure of formation, described
Cavity 23 is formed between body 21 and the disk 22, the ontology 21 is formed detachably with the disk 22 by fixed structure 5
Be fixedly connected, in order to ensure the use reliability of air sparger 2, prevent the air sparger 2 to be corroded or gas leakage, institute
It states ontology 21 to be made of metal material, it is preferable that the ontology 21 is by high-speed tool bracing members.The interface structure 211 and institute
It is separate structure to state ontology 21, and the interface structure 211 and the ontology 21 are preferably fixed together by welding manner.It is described
Interface structure 211 and the ontology 21 enable to the scope of application of the air sparger 2 wider using separate structure, can be with
It needs the device connected different according to the air sparger 2, the various sizes of interface knot is welded on the ontology 21
Structure.It is, of course, understood that the ontology 21 and the interface structure 211 can also be structure as a whole, casting can be passed through
It is integrally formed, in order to process.The gas in the air sparger 2 is entered from the interface structure 211, it can be in institute
It states and stores a period of time in the cavity 23 formed between ontology 21 and the disk 22, it is therefore preferable that can be in the cavity 23
Middle setting is entered with improving in the tank body 1 such as with filtering function or with the device for increasing oxygen content in gas
The cleanliness factor and oxygen content of gas, to realize better cultivate effect so that the adventitious root quality higher cultivated.
Wherein, as shown in Fig. 2, the fixed structure 5 includes the first fixing piece 51 and the second fixing piece 52, described first is solid
Determine part 51 to be connected with the ontology 21 and be located at the side far from the disk 22 on the ontology 21, second fixing piece
52 are connected with the disk 22 and are located at the side far from the ontology 21 in the disk 22,51 He of the first fixing piece
Second fixing piece 52 is fixed together by fastener (not shown), and the ontology 21 and the disk 22 are pressed from both sides
Tightly between first fixing piece 51 and second fixing piece 52.First fixing piece 51 and second fixing piece 52
Structure annular in shape, first fixing piece 51 and second fixing piece 52 are located at the ontology 21 and the disk 22
Edge.Preferably, the fastener uses screw and bolt, to ensure the convenience of disassembly and installation, can also ensure that simultaneously
Good fastening effect.The ontology 21 is fixed together with the disk 22 by the fixed structure 5, can be very convenient
Ground is disassembled and installed, in order to which it is cleaned or is overhauled after a period of use in the air sparger 2, to protect
Demonstrate,prove the using effect and use reliability of the air sparger 2.
As shown in Figure 1, the bioreactor further includes agitating device 6, the agitating device 6 includes by the tank body 1
The column structure 61 that extends to the bottom of the tank body 1 of top and power part 52.The power part 62 and the column
The one end for being located at 1 top of the tank body in body structure 61 is fixedly connected, and the power part 62 drives the column structure 61 certainly
Turn and/or swing, to be disturbed to the adventitious root in the tank body 1.At interval of predetermined length set on the column structure 61
Equipped with propeller blade 63, when 61 rotation of column structure, the propeller blade 63 is in the cylinder
Rotation under the drive of structure 61；When drive lower swing of the column structure 61 in the power part 62, the propeller
Formula stirring blade 63 is also swung while rotation.Preferably, the power part 62 includes motor, and the motor is preferred
For can either output torque can export the motor of amplitude again, drive the column structure to rotate and vibration to realize simultaneously.Its
In, the shape of the column structure 61 does not limit, and can may be square column for cylinder, Special-Shaped Column be can also be, with convenient
Installing and using is standard.The column structure 61 extends to the specific location of the bottom of the tank body 1 according to the tank body 1
Volume and height are configured, it is preferable that one end that the column structure 61 is located at the bottom of the tank body 1 is set to the tank
At the boundary position of the wall structure 12 of ontology 11 and the back taper, to ensure the adventitious root of each position in the tank body 1
Disturbance can be experienced.The quantity of the propeller blade 63 should not be excessive, adjacent two propeller types
The distance between stirring blade 63 should not be too large, to prevent from generating unnecessary injury to adventitious root.Since stirring blade is spiral shell
The blade construction of rotary propeller type can effectively reduce the propeller blade 63 and reduce in its rotation and swing process
Shearing force ensures to cultivate efficiency and cultivates quality to the mechanical wounding of the adventitious root of ginseng.Meanwhile it being stirred by the propeller type
Dissolved oxygen environment in culture solution in the tank body 1 can also be enhanced by mixing the rotation of blade 63, created better culture environment, trained
Raise the adventitious root of better quality.
Further, as adventitious root is constantly grown up, the volume and weight of adventitious root is increasing, adjacent adventitious root meeting
Mutual continuous weave in needs to grow into the tank body 1 a degree of to obtain the adventitious root having good quality
Adventitious root, which is transferred in the bioreactor of volume bigger, continue culture breeding.Therefore, institute is located on the column structure
The one end for stating the bottom of tank body 1 is provided with propeller type shear-blade 64, during the 61 continuous rotation of column structure, institute
It states propeller type shear-blade 64 constantly to rotate, to be sheared to adventitious root, be shifted the adventitious root shear off with facilitating
To being cultivated in the bioreactor of bigger.The air sparger to being passed through gas inside the tank body 1 from bottom to top, institute
State the adventitious root of 1 bottom of tank body becomes dispersity, the propeller blade under the drive of air-flow from coherent condition
The dispersion effect of adventitious roots is reinforced in 63 rotations, and in the process, the propeller type shear-blade 64 rotates, to it is scattered not
Determine root to be sheared, to realize better shear effect.Preferably, there are four shear blades for the tool of propeller type shear-blade 64
641, to ensure simultaneously to shear the adventitious root of multiple directions, shear effect is more preferable.
The adventitious root shear off is shifted for convenience, is arranged on the wall structure 12 of the bottom of the tank body 1
There is transfer outlet (not shown), the transfer outlet is for the adventitious root will be located at the bottom of the tank body 1 from the tank
It removes, and then is transferred in the reactor of bigger in body 1.It is provided with inoculation on the wall structure 12 at the top of the tank body 1
Entrance 13, the inoculation entrance 13 is for being inoculated with the adventitious root into the tank body 1.
Further, outlet passageway structure 7, the outlet are provided on the wall structure 12 at the top of the tank body 1
Channel design 7 includes vertically extending condenser pipe 71.The outlet passageway structure 7 can will be in adventitious root growth and development process
The metabolism gas of generation obtains preferably culture from 1 inside discharge of the tank body to ensure the cleaning of environment in the tank body 1
Breed environment.The condenser pipe 71 gas being discharged from the outlet passageway structure 7 can be carried out condensation and by condensed water from
It is discharged in the condenser pipe 71.
Embodiment 2
Difference lies in pass through welding side between the ontology and disk of air sparger between the present embodiment and embodiment 1
Formula or fastener are fixed together, without being fixed by fixed structure.
In addition, it is provided with filter device in the outlet passageway structure in the present embodiment, the filter device includes
Drying section and adsorption section, the drying section are used for being adsorbed from the steam in the gas being discharged in tank body, to ensure to be discharged
Gas outside to the tank body is all dry, prevents the environment outside tank body excessively moist.The adsorption section be used for from
Harmful substance in the gas being discharged in the tank body is adsorbed, to ensure that the air being discharged to outside the tank body is all by net
Change, not can cause environmental pollution.
Claims (10)
1. a kind of bioreactor is cultivated for the adventitious root to biology, the reactor includes tank body, the tank body
Bottom is provided with air sparger, it is characterised in that：The air sparger includes the ontology snapped together and disk, described
Ontology includes the interface structure being connected to reactor outside, and the gas outside the reactor passes sequentially through the interface knot
Structure, the disk enter in the tank body, and the disk includes at least metal sintering stratum reticulare.
2. bioreactor as described in claim 1, it is characterised in that：The ontology includes the middle part song that recess is formed downwards
Face structure, forms cavity between the ontology and the disk, the ontology is formed with the disk by fixed structure removable
That unloads is fixedly connected, and/or,
The ontology is made of metal material.
3. bioreactor as claimed in claim 2, it is characterised in that：The fixed structure includes the first fixing piece and second
Fixing piece, first fixing piece is connected with the ontology and is located at the side far from the disk on the ontology, described
Second fixing piece be connected with the disk and be located in the disk far from the ontology side, first fixing piece and
Second fixing piece is fixed together by fastener, and the ontology and the disk are clamped in first fixing piece
Between second fixing piece.
4. bioreactor as claimed in claim 3, it is characterised in that：First fixing piece and second fixing piece are in
Cyclic structure, first fixing piece and second fixing piece are located at the edge of the ontology and the disk.
5. bioreactor as described in claim 1, it is characterised in that:The bioreactor further includes agitating device, institute
It includes the column structure extended from the top of the tank body to the bottom of the tank body to state agitating device,
On the column structure propeller blade is arranged at interval of predetermined length.
6. bioreactor as claimed in claim 5, it is characterised in that：It is located at the bottom of the tank body on the column structure
One end be provided with propeller type shear-blade.
7. bioreactor as claimed in claim 6, it is characterised in that：The agitating device further includes power part, described
Power part is fixedly connected with the one end for being located at the top of the tank on the column structure, and the power part drives the column
The rotation of body structure and/or swing, to be disturbed to the adventitious root in the tank body.
8. bioreactor as described in claim 1, it is characterised in that:The wall structure of the bottom of the tank body is in back taper
Shape, the air sparger are arranged in the bottom end of the back taper, and the interface structure extend out to the outside of the tank body and leads to
Air pipe is connected.
9. bioreactor as described in claim 1, it is characterised in that：It is provided on the wall structure of the bottom of the tank body
Transfer outlet, the transfer outlet are used to remove the adventitious root for being located at the bottom of the tank body from the tank body, and/or,
Inoculation entrance is provided on the wall structure at the top of the tank body, the inoculation entrance into the tank body for being inoculated with
The adventitious root.
10. bioreactor as described in claim 1, it is characterised in that：It is arranged on the wall structure at the top of the tank body
There is an outlet passageway structure, the outlet passageway structure includes vertically extending condenser pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810398406.8A CN108522247A (en) | 2018-04-28 | 2018-04-28 | A kind of bioreactor of culture ginseng adventitious root |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810398406.8A CN108522247A (en) | 2018-04-28 | 2018-04-28 | A kind of bioreactor of culture ginseng adventitious root |
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| Publication Number | Publication Date |
|---|---|
| CN108522247A true CN108522247A (en) | 2018-09-14 |
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| CN201810398406.8A Pending CN108522247A (en) | 2018-04-28 | 2018-04-28 | A kind of bioreactor of culture ginseng adventitious root |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022206423A1 (en) * | 2021-04-02 | 2022-10-06 | 山东安然纳米实业发展有限公司 | Biological reaction device and culture method for adventitious roots of panax ginseng c. a. mey. |
| CN117256478A (en) * | 2023-11-22 | 2023-12-22 | 上海科缔联科技有限公司 | Continuous fermentation culture system for plant tissues |
| CN117256479A (en) * | 2023-11-22 | 2023-12-22 | 上海科缔联科技有限公司 | Industrial automatic biological reaction device and control method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022206423A1 (en) * | 2021-04-02 | 2022-10-06 | 山东安然纳米实业发展有限公司 | Biological reaction device and culture method for adventitious roots of panax ginseng c. a. mey. |
| CN117256478A (en) * | 2023-11-22 | 2023-12-22 | 上海科缔联科技有限公司 | Continuous fermentation culture system for plant tissues |
| CN117256479A (en) * | 2023-11-22 | 2023-12-22 | 上海科缔联科技有限公司 | Industrial automatic biological reaction device and control method thereof |
| CN117256478B (en) * | 2023-11-22 | 2024-02-02 | 上海科缔联科技有限公司 | Continuous fermentation culture system for plant tissues |
| CN117256479B (en) * | 2023-11-22 | 2024-02-06 | 上海科缔联科技有限公司 | Industrial automatic biological reaction device and control method thereof |
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