CN208462631U - A kind of bioreactor - Google Patents

A kind of bioreactor Download PDF

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Publication number
CN208462631U
CN208462631U CN201820625737.6U CN201820625737U CN208462631U CN 208462631 U CN208462631 U CN 208462631U CN 201820625737 U CN201820625737 U CN 201820625737U CN 208462631 U CN208462631 U CN 208462631U
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tank body
ontology
bioreactor
disk
fixing piece
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CN201820625737.6U
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刘汉石
刘禹
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

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Abstract

The utility model provides a kind of bioreactor, it is cultivated for the adventitious root to biology, reactor includes tank body, the bottom of tank body is provided with air sparger, air sparger includes the ontology snapped together and disk, and ontology includes the interface structure being connected to reactor outside, and the gas outside reactor passes sequentially through interface structure, disk enters in tank body, disk includes at least metal sintering stratum reticulare, and metal sintering stratum reticulare includes at least filter layer.Bioreactor provided by the utility model can realize that large-scale breeding is cultivated to ginseng adventitious root, to produce the industrialization ginseng adventitious root product that mass is stable, qualification rate is high.Bioreactor in the utility model, which only needs to carry out certain transformation on general fermentor, can be used to ginseng adventitious root breeding, and reactor low cost is easy to get, and reduces the production cost in ginseng adventitious root cultivating process, and large-scale promotion is suitble to use.

Description

A kind of bioreactor
Technical field
The utility model relates to biological breeding apparatus fields, and in particular to a kind of bioreactor.
Background technique
With the development of society and the improvement of people's living standards, people are higher and higher to the attention degree of health.People Ginseng is increasingly pursued as a kind of holy product of nourishing.The training method of ginseng is carried out mostly with culturing and transplanting seedlings or direct-seeding, The speed of growth is slow, is unable to satisfy people to ginseng and the ever-increasing demand of ginseng adventitious root.
Laboratory stage is substantially in the culture of ginseng adventitious root in the prior art, in the mistake cultivated it In journey using to reactor be laboratory scale small-sized biological reactor, use reactor carry out single culture reaction Body product is between 5-20 liter.These experiments are mostly glass gas lift tank or PC gas lift bucket with small-sized biological reactor, not only body Product is small, and equipment is simple, cannot achieve and efficiently cultivates ginseng adventitious root.
Utility model content
The purpose of this utility model is that being asked for ginseng adventitious root breeding amount is small in the prior art, reproductive efficiency is low Topic provides the bioreactor that a kind of culture scale is big, culture efficiency is high, the adventitious root qualification rate of culture is high.
In order to solve the above technical problems, the utility model adopts the following technical solution:
A kind of bioreactor is cultivated for the adventitious root to biology, and the reactor includes tank body, the tank body Bottom be provided with air sparger, the air sparger includes the ontology snapped together and disk, and the ontology includes With the interface structure being connected to outside the reactor, the gas outside the reactor passes sequentially through the interface structure, described Disk enters in the tank body, and the disk sintered metal mesh is made, and the metal sintering stratum reticulare includes at least filter layer.
Preferably, the ontology includes the middle part curved-surface structure that recess is formed downwards, between the ontology and the disk Cavity is formed, the ontology is formed by fixed structure with the disk and is removably fixedly connected, and/or,
The ontology is made of metal material.
Preferably, the fixed structure include the first fixing piece and the second fixing piece, first fixing piece with described Body is connected and is located at the side on the ontology far from the disk, and second fixing piece is connected with the disk and position In, far from the side of the ontology, first fixing piece and second fixing piece are fixed on by fastener in the disk Together, the ontology and the disk are clamped between first fixing piece and second fixing piece.
Preferably, first fixing piece and the second fixing piece structure annular in shape, first fixing piece and described Second fixing piece is located at the edge of the ontology and the disk.
Preferably, the bioreactor further includes agitating device, and the agitating device includes by the top of the tank body The column structure extended to the bottom of the tank body,
Propeller blade is arranged at interval of predetermined length on the column structure.
Preferably, one end of the bottom on the column structure positioned at the tank body is provided with propeller type shear-blade.
Preferably, the agitating device further includes power part, and institute is located on the power part and the column structure The one end for stating top of the tank is fixedly connected, and the power part drives the column structure rotation and/or swing, to the tank Intracorporal adventitious root is disturbed.
Preferably, the wall structure of the bottom of the tank body is in back taper, and the air sparger is arranged in the back taper The bottom end of shape, the outside that the interface structure extend out to the tank body are connected with vent line.
Preferably, it is provided with transfer outlet on the wall structure of the bottom of the tank body, the transfer is exported for by position Adventitious root in the bottom of the tank body is removed from the tank body, and/or,
Inoculation entrance is provided on the wall structure at the top of the tank body, the inoculation entrance is used for into the tank body It is inoculated with the adventitious root.
Preferably, outlet passageway structure, the outlet passageway structure are provided on the wall structure at the top of the tank body Including vertically extending condenser pipe.
Bioreactor provided by the utility model can realize that large-scale breeding is cultivated to ginseng adventitious root, to produce The industrialization ginseng adventitious root product that quality is stable, qualification rate is high.
Bioreactor in the utility model, which only needs to carry out certain transformation on general fermentor, can be used to people Join indefinite root propagation, reactor low cost is easy to get, and reduces the production cost in ginseng adventitious root cultivating process, is suitble to Large-scale promotion uses.
Detailed description of the invention
Fig. 1 shows the overall structure diagram of the bioreactor of the offer of the utility model embodiment 1;
Fig. 2 shows the mounting structure schematic diagram of the fixed structure of the offer of the utility model embodiment 1.
Wherein,
1. tank body, 11. tank ontologies, 12. wall structures, 13. inoculation entrances,
2. air sparger, 21. ontologies, 211. interface structures, 22. disks, 23. cavitys,
3. bracket,
4. vent line,
5. fixed structure, 51. first fixing pieces, 52. second fixing pieces,
6. agitating device, 61. column structures, 62. power parts, 63. propeller blades, 64. propeller types are cut Cutting edge, 641. shear blades,
7. outlet passageway structure, 71. condenser pipes.
Specific embodiment
Bioreactor provided by the utility model is described in more detail with reference to the accompanying drawing.
Illustrate: upper and lower on the basis of direction shown in FIG. 1 during the utility model description.
Embodiment 1
As depicted in figs. 1 and 2, a kind of bioreactor is present embodiments provided, is trained for the adventitious root to biology It supports, such as the adventitious root of ginseng.The reactor includes tank body 1, and the tank body 1 includes tank ontology 11, and the tank body 1 is by metal Material is made, it is preferred to use high temperature resistant, corrosion resistant metal material, such as aluminium alloy or high-speed tool steel.The bottom of the tank body 1 The wall structure 12 in portion be in back taper, in the tank body 1 of back taper bottom wall structure 12 and the tank ontology 11 it Between can be structure as a whole or separate structure, when it is both above-mentioned be separate structure when, it is preferred to use welding manner is by two Person is fixed together.Be additionally provided with bracket 3 in the bottom of the tank body 1, to be supported to the tank body 1, the bracket 3 with It is preferably fixed together using welding manner between the tank body 1, to enhance intensity between the two, is prevented in the tank body 1 Weight is larger after culture solution and adventitious root is added, and the bracket 3 is because that the problem of damage can not occur due to load-bearing.
Further, the bottom of the tank body 1 is provided with air sparger 2, in the present embodiment, the air sparger 2 are arranged in the bottom end of the back taper.It is described since the adventitious root in the tank body 1 is gradually grown up during the cultivation process The weight of adventitious root is also to gradually increase, and therefore, the adventitious root can decline in its own gravity.By the tank body 1 The wall structure 12 of bottom be set as back taper, can allow the overall compact bottom for concentrating on back taper wall structure 12 of adventitious root Portion.Simultaneously as the bottom end of the back taper is arranged in the air sparger 2, so that the air sparger 2 was produced by boasting The adventitious root for being deposited in 1 bottom of tank body can preferably be blowed to the middle and upper part of the tank body 1 by bubble again, and it is indefinite to guarantee Root disperse in the tank body 1 it is more uniform, to guarantee that it is balanced that each adventitious root can be absorbed into the tank body 1 Nutrition, and then guarantee to cultivate reliability, improve the cultivation qualification rate of adventitious root.
The air sparger 2 includes the ontology 21 that snaps together and disk 22, the ontology 21 include with it is described anti- The interface structure 211 being connected to outside device is answered, the outside that the interface structure 211 extend out to the tank body 1 is connected with vent line 4 It connects, clean air, or other gas needed in adventitious root incubation can be passed through in the vent line 4 Body.Control valve (not shown), the control of the control valve and the bioreactor are provided in the interface structure 211 Device (not shown) processed is connected, and the control device can control the opening and closing of the control valve to control the interface knot The on-off of air-flow in structure 211.Gas outside the reactor passes sequentially through the interface structure 211, the disk 22 enters Into the tank body 1, the disk 22 includes metal sintering stratum reticulare, and metal sintering stratum reticulare is by sintered metal mesh materials for support.Institute It states metal sintering stratum reticulare and is combined using the wire of multilayer by the techniques such as special lamination compacting, vacuum-sintering and is fabricated 's.The metal sintering stratum reticulare includes five layers, filter layer, protective layer, supporting course, separating layer and enhancement layer, in the present embodiment Metal material for manufacturing the metal sintering stratum reticulare is preferably high-speed tool steel.The metal sintering stratum reticulare is existing uniformly steady Fixed filtering accuracy has higher intensity and rigidity, while compression strength also with higher and grade of filtration again.The metal Stratum reticulare is sintered since its work principle of filter is both surface filtration, and it is smooth by the mesh duct that wire is formed, therefore it is with excellent Backwash and regeneration performance, can be used for a long time repeatedly, can be used the methods of counter-flow water, filtrate, ultrasonic wave, melting, baking cleaning It is cleaned.Meanwhile the metal sintering net materials for forming the metal sintering stratum reticulare are easy to form, can add to it Work and welding operation, manufacture the various forms of filter elements such as circle, tubular, cone cell, corrugated in which can be convenient.Institute State having wide range of applications for sintered metal mesh, can be used in -200 DEG C~600 DEG C temperature environment and acid or alkali environment into Row filtering, is particularly suitable for the operating process of serialization and automation, any filtering material can not all be compared.
Further, the ontology 21 of the air sparger 2 includes that middle part is recessed downwards the curved-surface structure of formation, and described Cavity 23 is formed between body 21 and the disk 22, the ontology 21 is formed detachably with the disk 22 by fixed structure 5 Be fixedly connected, in order to guarantee the use reliability of air sparger 2, prevent the air sparger 2 to be corroded or gas leakage, institute It states ontology 21 to be made of metal material, it is preferable that the ontology 21 is by high-speed tool bracing members.The interface structure 211 and institute Stating ontology 21 is separate structure, and the interface structure 211 and the ontology 21 are preferably fixed together by welding manner.Institute It states interface structure 211 and the ontology 21 and enables to the scope of application of the air sparger 2 wider using separate structure, The device that connect can be needed different according to the air sparger 2, various sizes of described connect is welded on the ontology 21 Mouth structure.It is, of course, understood that the ontology 21 and the interface structure 211 can also be structure as a whole, Ke Yitong Casting integrated molding is crossed, in order to process.The gas in the air sparger 2 is entered from the interface structure 211, A period of time is stored in the cavity 23 that can be formed between the ontology 21 and the disk 22, it is therefore preferable that can be described It is arranged in cavity 23 such as with filtering function or with the device for increasing oxygen content in gas, enters the tank body to improve The cleanliness and oxygen content of gas in 1, to realize better cultivate effect, so that the adventitious root quality cultivated is higher.
Wherein, as shown in Fig. 2, the fixed structure 5 include the first fixing piece 51 and the second fixing piece 52, described first Fixing piece 51 is connected with the ontology 21 and is located at the side on the ontology 21 far from the disk 22, and described second is fixed Part 52 is connected with the disk 22 and is located at the side in the disk 22 far from the ontology 21, first fixing piece 51 and second fixing piece 52 be fixed together by fastener (not shown), by the ontology 21 and the disk 22 are clamped between first fixing piece 51 and second fixing piece 52.First fixing piece 51 and described second is fixed The structure annular in shape of part 52, first fixing piece 51 and second fixing piece 52 are located at the ontology 21 and the disk 22 edge.Preferably, the fastener uses screw and bolt, to guarantee the convenience of disassembly and installation, while can also Guarantee good fastening effect.The ontology 21 is fixed together with the disk 22 by the fixed structure 5, Neng Goufei It is often easily disassembled and installed, in order to which it is cleaned or is examined after a period of use in the air sparger 2 It repairs, to guarantee the using effect and use reliability of the air sparger 2.
As shown in Figure 1, the bioreactor further includes agitating device 6, the agitating device 6 includes by the tank body 1 The top column structure 61 and power part 52 that extend to the bottom of the tank body 1.The power part 62 with it is described The one end for being located at 1 top of tank body on column structure 61 is fixedly connected, and the power part 62 drives the column structure 61 Rotation and/or swing, to be disturbed to the adventitious root in the tank body 1.At interval of predetermined length on the column structure 61 It is arranged with propeller blade 63, when 61 rotation of column structure, the propeller blade 63 is in the column Rotation under the drive of body structure 61;When drive lower swing of the column structure 61 in the power part 62, the spiral Paddle stirring blade 63 is also swung while rotation.Preferably, the power part 62 includes motor, and the motor is excellent Be selected as can either output torque can export the motor of amplitude again, drive the column structure rotation and vibration to realize simultaneously. Wherein, the shape of the column structure 61 does not limit, and can may be square column for cylinder, can also be Special-Shaped Column, with side Just installing and use is standard.The column structure 61 extends to the specific location of the bottom of the tank body 1 according to the tank body 1 Volume and height be configured, it is preferable that described in one end that the column structure 61 is located at the bottom of the tank body 1 is set to At the boundary position of the wall structure 12 of tank ontology 11 and the back taper, to guarantee the indefinite of each position in the tank body 1 Root can experience disturbance.The quantity of the propeller blade 63 should not be excessive, two adjacent propellers The distance between formula stirring blade 63 should not be too large, to prevent from generating unnecessary injury to adventitious root.Since stirring blade is The blade construction of propeller type can effectively reduce the propeller blade 63 and subtract in its rotation and swing process Small shearing force guarantees to cultivate efficiency and cultivates quality to the mechanical wounding of the adventitious root of ginseng.Meanwhile passing through the propeller type The rotation of stirring blade 63 can also enhance the dissolved oxygen environment in the culture solution in the tank body 1, create better culture environment, Turn out the adventitious root of better quality.
Further, as adventitious root is constantly grown up, the volume and weight of adventitious root is increasing, adjacent adventitious root meeting Mutual continuous weave in needs to grow into the tank body 1 a degree of to obtain the adventitious root having good quality Adventitious root, which is transferred in the bigger bioreactor of volume, continue culture breeding.Therefore, institute is located on the column structure The one end for stating the bottom of tank body 1 is provided with propeller type shear-blade 64, during the 61 continuous rotation of column structure, institute It states propeller type shear-blade 64 constantly to rotate, to be sheared to adventitious root, be shifted the adventitious root shear off with facilitating It is cultivated into bigger bioreactor.The air sparger to being passed through gas inside the tank body 1 from bottom to top, institute The adventitious root for stating 1 bottom of tank body becomes dispersity, the propeller blade from coherent condition under the drive of air-flow The dispersion effect of adventitious roots is reinforced in 63 rotations, and in the process, the propeller type shear-blade 64 rotates, to it is scattered not Determine root to be sheared, to realize better shear effect.Preferably, there are four shear blades for the tool of propeller type shear-blade 64 641, to guarantee simultaneously to shear the adventitious root of multiple directions, shear effect is more preferable.
The adventitious root shear off is shifted for convenience, is arranged on the wall structure 12 of the bottom of the tank body 1 There is transfer outlet (not shown), the transfer exports the adventitious root for that will be located at the bottom of the tank body 1 from the tank It removes, and then is transferred in bigger reactor in body 1.Inoculation is provided on the wall structure 12 at the top of the tank body 1 Entrance 13, the inoculation entrance 13 is for being inoculated with the adventitious root into the tank body 1.
Further, outlet passageway structure 7, the outlet are provided on the wall structure 12 at the top of the tank body 1 Channel design 7 includes vertically extending condenser pipe 71.The outlet passageway structure 7 can will be in adventitious root growth and development process The metabolism gas of generation is discharged inside the tank body 1, to guarantee the cleaning of environment in the tank body 1, obtains preferably culture Breed environment.The condenser pipe 71 gas being discharged from the outlet passageway structure 7 can be carried out condensation and by condensed water from It is discharged in the condenser pipe 71.
Embodiment 2
Difference between the present embodiment and embodiment 1 is, passes through welding side between the ontology and disk of air sparger Formula or fastener are fixed together, without being fixed by fixed structure.
In addition, it is provided with filter device in the outlet passageway structure in the present embodiment, the filter device includes Drying section and adsorption section, the drying section to from the steam in the gas being discharged in tank body for adsorbing, to guarantee to be discharged Gas outside to the tank body be all it is dry, prevent the environment outside tank body excessively moist.The adsorption section be used for from The harmful substance in gas being discharged in the tank body is adsorbed, to guarantee that the air being discharged to outside the tank body is all by net Change, not can cause environmental pollution.

Claims (10)

1. a kind of bioreactor is cultivated for the adventitious root to biology, the reactor includes tank body, the tank body Bottom is provided with air sparger, it is characterised in that: the air sparger includes the ontology snapped together and disk, described Ontology includes the interface structure being connected to outside the reactor, and the gas outside the reactor passes sequentially through the interface knot Structure, the disk enter in the tank body, and the disk includes at least metal sintering stratum reticulare, and the metal sintering stratum reticulare is at least Including filter layer.
2. bioreactor as described in claim 1, it is characterised in that: the ontology includes the middle part song that recess is formed downwards Face structure, forms cavity between the ontology and the disk, the ontology is formed with the disk by fixed structure removable That unloads is fixedly connected, and/or,
The ontology is made of metal material.
3. bioreactor as claimed in claim 2, it is characterised in that: the fixed structure includes the first fixing piece and second Fixing piece, first fixing piece is connected with the ontology and is located at the side on the ontology far from the disk, described Second fixing piece be connected with the disk and be located in the disk far from the ontology side, first fixing piece and Second fixing piece is fixed together by fastener, and the ontology and the disk are clamped in first fixing piece Between second fixing piece.
4. bioreactor as claimed in claim 3, it is characterised in that: first fixing piece and second fixing piece are in Cyclic structure, first fixing piece and second fixing piece are located at the edge of the ontology and the disk.
5. bioreactor as described in claim 1, it is characterised in that: the bioreactor further includes agitating device, institute Stating agitating device includes the column structure extended from the top of the tank body to the bottom of the tank body,
Propeller blade is arranged at interval of predetermined length on the column structure.
6. bioreactor as claimed in claim 5, it is characterised in that: be located at the bottom of the tank body on the column structure One end be provided with propeller type shear-blade.
7. bioreactor as claimed in claim 6, it is characterised in that: the agitating device further includes power part, described Power part is fixedly connected with the one end for being located at the top of the tank on the column structure, and the power part drives the column The rotation of body structure and/or swing, to be disturbed to the intracorporal adventitious root of the tank.
8. bioreactor as described in claim 1, it is characterised in that: the wall structure of the bottom of the tank body is in back taper The bottom end of the back taper is arranged in shape, the air sparger, and the interface structure extend out to the outside of the tank body and leads to Air pipe is connected.
9. bioreactor as described in claim 1, it is characterised in that: be provided on the wall structure of the bottom of the tank body Transfer outlet, the transfer outlet are used to remove the adventitious root for being located at the bottom of the tank body from the tank body, and/or,
Inoculation entrance is provided on the wall structure at the top of the tank body, the inoculation entrance into the tank body for being inoculated with The adventitious root.
10. bioreactor as described in claim 1, it is characterised in that: be arranged on the wall structure at the top of the tank body There is outlet passageway structure, the outlet passageway structure includes vertically extending condenser pipe.
CN201820625737.6U 2018-04-28 2018-04-28 A kind of bioreactor Active CN208462631U (en)

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Application Number Priority Date Filing Date Title
CN201820625737.6U CN208462631U (en) 2018-04-28 2018-04-28 A kind of bioreactor

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Application Number Priority Date Filing Date Title
CN201820625737.6U CN208462631U (en) 2018-04-28 2018-04-28 A kind of bioreactor

Publications (1)

Publication Number Publication Date
CN208462631U true CN208462631U (en) 2019-02-05

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