A kind of bioreactor for cultivating plant cell
Technical field
The utility model relates to plant cells to cultivate field, and in particular to a kind of bioreactor.
Background technique
With the development of society and the improvement of people's living standards, people are higher and higher to the attention degree of health.In
Medicine is as the time-honored traditional medicine culture in China, the deep love and esteem by the numerous people masses.Chinese medicine due to its Small side effects,
Also it is widely used in dietotherapy field, the taste of the distinctive taste of Chinese medicine and food is fused together, such as Radix Angelicae Sinensis, fructus lycii etc.
Chinese medicine is just often used as the material of Baoshang, so that the soup stewed out is not only nutritious but also delicious flavour.But due to most of
The training method of Chinese medicine is to be carried out with culturing and transplanting seedlings or direct-seeding, and the speed of growth is slow, is unable to satisfy people and increases Chinese medicine
Demand, and then lead to the price virtual height of some Chinese medicines.
In the prior art to the culture of the plants such as Chinese herbal medicine, need to use in incubation since culture plant cell
Culture medium all in the experimental stage, carry out the reactor volume of single culture between 5-20 liter using reactor.To it
The reactor used during being cultivated is based on shaking table triangular flask or culture bag or microbe fermentor
It is cultivated, cannot achieve the effect efficiently cultivated plant cell, and training quality is barely satisfactory always.
Utility model content
The purpose of this utility model is that being asked for plant cell breeding amount is small in the prior art, reproductive efficiency is low
Topic provides the bioreactor that a kind of culture scale is big, culture efficiency is high, the cell line qualification rate of culture is high.
In order to solve the above technical problems, the utility model adopts the following technical solution:
A kind of bioreactor, for cultivating plant cell, the reactor includes tank body, in the tank body
It is provided with light source generator, the light source generator is arranged in the outside wall surface of the tank body by mounting structure, described
Light source generator include light source and be arranged in outside the light source for protecting the protective jacket of the light source, the light source and supply
Electric line is connected.
Preferably, the light source generator is elongated, and one end of the protective jacket is fixed on described by mounting structure
In the outside wall surface of tank body, the other end of the protective jacket is extend into the tank body;
The mounting structure includes cassette container, and casing seat is provided on the protective jacket, and described sleeve pipe seat snaps onto institute
It states in cassette container, outside wall surface of the cassette container also with the tank body is fastenedly connected, so that the protective jacket and the tank
Body is fixed together.
Preferably, the protective jacket is open at one end, and other end closing, the light source is inserted into from the open end of the protective jacket
Into the protective jacket, it is provided with pressure ring between the inner wall and the light source at the aperture position of the protective jacket, it will be described
Light source is fixed in the protective jacket, and the shield abutted against with it is provided with above the light source, is set above the shield
It is equipped with the end cap for closing the protective jacket.
Preferably, the bottom of the tank body is provided with air sparger, and the air sparger includes snapping together
Ontology and disk, the ontology include with the interface structure that is connected to outside the reactor, gas outside the reactor according to
Secondary to be entered in the tank body by the interface structure, the disk, the disk includes at least metal sintering stratum reticulare.
Preferably, the ontology includes the middle part curved-surface structure that recess is formed downwards, between the ontology and the disk
Cavity is formed, the ontology is formed by fixed structure with the disk and is removably fixedly connected.
Preferably, the fixed structure include the first fixing piece and the second fixing piece, first fixing piece with described
Body is connected and is located at the side on the ontology far from the disk, and second fixing piece is connected with the disk and position
In, far from the side of the ontology, first fixing piece and second fixing piece are fixed on by fastener in the disk
Together, the ontology and the disk are clamped between first fixing piece and second fixing piece.
Preferably, first fixing piece and the second fixing piece structure annular in shape, first fixing piece and described
Second fixing piece is located at the edge of the ontology and the disk.
Preferably, the wall structure of the bottom of the tank body is in back taper, and the air sparger is arranged in the back taper
The bottom end of shape, the outside that the interface structure extend out to the tank body are connected with vent line.
Preferably, the bioreactor further includes agitating device, and the agitating device includes by the top of the tank body
To the column structure of the bottom of tank body extension, propeller is arranged at interval of predetermined length on the column structure
Blade.
Preferably, the agitating device further includes power part, and institute is located on the power part and the column structure
The one end for stating top of the tank is fixedly connected, and the power part drives the column structure rotation and/or swing, to the tank
Intracorporal cell line is disturbed.
Bioreactor provided by the utility model can realize that large-scale breeding is cultivated to plant cell, to produce
The important plant cell product of the industrialization that quality is stable, qualification rate is high, with provided to market stablize it is qualified it is important effectively at
Point.
Bioreactor in the utility model, which only needs to carry out certain transformation on general fermentor, can be used to plant
The breeding of object cell line, reactor low cost are easy to get, and reduce the production cost in plant cell cultivating process, are suitble to
Large-scale promotion uses.
Detailed description of the invention
Fig. 1 shows the overall structure diagram of the bioreactor of the offer of the utility model embodiment 1;
Fig. 2 shows the mounting structure schematic diagram of the fixed structure of the offer of the utility model embodiment 1;
Fig. 3 shows the structural schematic diagram of the light source generator of the offer of the utility model embodiment 1.
Wherein,
1. tank body, 11. tank ontologies, 12. wall structures, 13. inoculation entrances,
2. air sparger, 21. ontologies, 211. interface structures, 22. disks, 23. cavitys,
3. bracket,
4. vent line,
5. fixed structure, 51. first fixing pieces, 52. second fixing pieces,
6. agitating device, 61. column structures, 62. power parts, 63. propeller blades,
7. outlet passageway structure, 71. condenser pipes,
8. light source generator, 81. light sources, 82. protective jackets, 83. cassette containers, 84. casing seats, 85. pressure rings, 86. shields
Cover, 87. end caps, 88. sealing rings.
Specific embodiment
Feed packaging system provided by the utility model is described in more detail with reference to the accompanying drawing.
Illustrate: upper and lower on the basis of direction shown in FIG. 1 during the utility model description.
Embodiment 1
As depicted in figs. 1 and 2, a kind of bioreactor is present embodiments provided, is trained for the cell line to biology
It supports, such as the cell line of the Chinese medicines such as Radix Angelicae Sinensis, ginseng.The reactor includes tank body 1, and the tank body 1 includes tank ontology 11, institute
It states tank body 1 to be made of metal material, it is preferred to use high temperature resistant, corrosion resistant metal material, such as aluminium alloy or high-speed tool steel.
The wall structure 12 of the bottom of the tank body 1 is in back taper, wall structure 12 and institute in the bottom of the tank body 1 of back taper
Stating can be structure as a whole or separate structure between tank ontology 11, when it is both above-mentioned be separate structure when, it is preferred to use
The two is fixed together by welding manner.It is additionally provided with bracket 3 in the bottom of the tank body 1, to prop up the tank body 1
Support, is preferably fixed together using welding manner between the bracket 3 and the tank body 1, to enhance intensity between the two, is prevented
Weight is larger after culture solution and cell line only is added in the tank body 1, the bracket 3 because can not load-bearing due to asking for occurring damaging
Topic.
Further, the bottom of the tank body 1 is provided with air sparger 2, in the present embodiment, the air sparger
2 are arranged in the bottom end of the back taper.It is described since the cell line in the tank body 1 is gradually grown up during the cultivation process
The weight of cell line is also to gradually increase, and therefore, the cell line can decline under its own gravity.By the tank body
The wall structure 12 of 1 bottom is set as back taper, can allow the overall compact back taper wall structure 12 that concentrates on of cell line
Bottom.Simultaneously as the bottom end of the back taper is arranged in the air sparger 2, so that the air sparger 2 is produced by boasting
Bubble the cell line for being deposited in 1 bottom of tank body can be blowed to preferably to the middle and upper part of the tank body 1 again, guarantee thin
Born of the same parents tie up in the tank body 1 disperse it is more uniform, to guarantee that each cell line can be absorbed into equilibrium in the tank body 1
Nutrition, and then guarantee cultivate reliability, improve the cultivation qualification rate of cell line.
The air sparger 2 includes the ontology 21 that snaps together and disk 22, the ontology 21 include with it is described anti-
The interface structure 211 being connected to outside device is answered, the outside that the interface structure 211 extend out to the tank body 1 is connected with vent line 4
It connects, clean air, or other gas needed in cell line incubation can be passed through in the vent line 4
Body.Control valve (not shown), the control of the control valve and the bioreactor are provided in the interface structure 211
Device (not shown) processed is connected, and the control device can control the opening and closing of the control valve to control the interface knot
The on-off of air-flow in structure 211.Gas outside the reactor passes sequentially through the interface structure 211, the disk 22 enters
Into the tank body 1, the disk 22 includes at least metal sintering stratum reticulare.The metal sintering stratum reticulare uses the wire of multilayer
It combines by techniques such as special lamination compacting, vacuum-sinterings and to be fabricated.The metal sintering stratum reticulare includes five layers, mistake
Filtering layer, protective layer, supporting course, separating layer and enhancement layer, for manufacturing the metal of the metal sintering stratum reticulare in the present embodiment
Material is preferably high-speed tool steel.The existing uniform and stable filtering accuracy of metal sintering stratum reticulare have again higher intensity and just
Degree, while compression strength also with higher and grade of filtration.The metal sintering stratum reticulare is surface layer mistake due to its work principle of filter
Filter, and it is smooth by the mesh duct that wire is formed, therefore it can make for a long time repeatedly with excellent backwash and regeneration performance
With, can be used the methods of counter-flow water, filtrate, ultrasonic wave, melting, baking cleaning it is cleaned.Meanwhile forming the metal
The metal sintering net materials for being sintered stratum reticulare are easy to form, can be processed and be welded operation to it, with can be convenient the system of processing
It is made the various forms of filter elements such as circle, tubular, cone cell, corrugated.The sintered metal mesh has wide range of applications, energy
The temperature environment and acid or alkali environment for being enough used in -200 DEG C~600 DEG C are filtered, and are particularly suitable for serialization and automatic
The operating process of change, any filtering material can not all be compared.
Further, the ontology 21 of the air sparger 2 includes that middle part is recessed downwards the curved-surface structure of formation, and described
Cavity 23 is formed between body 21 and the disk 22, the ontology 21 is formed detachably with the disk 22 by fixed structure 5
Be fixedly connected, in order to guarantee the use reliability of air sparger 2, prevent the air sparger 2 to be corroded or gas leakage, institute
It states ontology 21 to be made of metal material, it is preferable that the ontology 21 is by high-speed tool bracing members.The interface structure 211 and institute
Stating ontology 21 is separate structure, and the interface structure 211 and the ontology 21 are preferably fixed together by welding manner.It is described
Interface structure 211 and the ontology 21 enable to the scope of application of the air sparger 2 wider using separate structure, can be with
The device for needing to connect according to the air sparger 2 is different, and the various sizes of interface knot is welded on the ontology 21
Structure.It is, of course, understood that the ontology 21 and the interface structure 211 can also be structure as a whole, casting can be passed through
It is integrally formed, in order to process.The gas in the air sparger 2 is entered from the interface structure 211, it can be in institute
It states and stores a period of time in the cavity 23 formed between ontology 21 and the disk 22, it is therefore preferable that can be in the cavity 23
Middle setting such as with filtering function or with the device for increasing oxygen content in gas, is entered in the tank body 1 with improving
The cleanliness and oxygen content of gas, to realize better cultivate effect, so that the cell line quality cultivated is higher.
Wherein, as shown in Fig. 2, the fixed structure 5 includes the first fixing piece 51 and the second fixing piece 52, described first is solid
Determine part 51 to be connected with the ontology 21 and be located at the side on the ontology 21 far from the disk 22, second fixing piece
52 are connected with the disk 22 and are located at the side in the disk 22 far from the ontology 21,51 He of the first fixing piece
Second fixing piece 52 is fixed together by fastener (not shown), and the ontology 21 and the disk 22 are pressed from both sides
Tightly between first fixing piece 51 and second fixing piece 52.First fixing piece 51 and second fixing piece 52
Structure annular in shape, first fixing piece 51 and second fixing piece 52 are located at the ontology 21 and the disk 22
Edge.Preferably, the fastener uses screw and bolt, to guarantee the convenience of disassembly and installation, can also ensure that simultaneously
Good fastening effect.The ontology 21 is fixed together with the disk 22 by the fixed structure 5, can be very convenient
Ground is disassembled and installed, in order to which it is cleaned or is overhauled after a period of use in the air sparger 2, to protect
Demonstrate,prove the using effect and use reliability of the air sparger 2.
Light is needed to irradiate since plant cell ties up in cultivating process, to guarantee the quality sum number for the cell line cultivated
Amount, therefore, as shown in figures 1 and 3, is provided with light source generator 8 in the tank body in the present embodiment.The light source occurs
Device 8 is arranged in the outside wall surface of the tank body 1 by mounting structure, and the light source generator 8 includes light source 81 and setting
The protective jacket 82 for being used to protect the light source 81 outside the light source 81.Specifically, the light source generator 8 is generally long
Shape, the protective jacket 82 and the light source 81 being set in the protective jacket 82 are also elongated.The protective jacket 82 is preferably using saturating
Bright glass material is manufactured, and can effectively pass through the protective jacket 82 to the tank body in the pipeline for guaranteeing the light source 81
Cell line in 1 is irradiated, while the light source 81 can also be effectively protected, and prevents the light source 81 by institute
The corrosion of culture solution in tank body 1 is stated, can also prevent the light source 81 from polluting to the culture solution in the tank body 1.Institute
The one end for stating protective jacket 82 is fixed in the outside wall surface of the tank body 1 by mounting structure, to facilitate the outside from the tank body 1
It is connected to the light source 81 access power supply line and is powered.The other end of the protective jacket 82 is extend into the tank body 1,
The luminous component of the light source 81 is located in the tank body 1, to provide needed for growth to the plant cell in the tank body 1
Illumination.Wherein, the mounting structure includes cassette container 83, and casing seat 84, described sleeve pipe seat are provided on the protective jacket 82
84 snap onto the cassette container 83, and outside wall surface of the cassette container 83 also with the tank body 1 is fastenedly connected, so that described
Protective jacket 83 and the tank body 1 are fixed together.The protective jacket 82 is open at one end, and other end closing, the light source 81 is from institute
The open end for stating protective jacket 82 is inserted into the protective jacket 82, the inner wall at the aperture position of the protective jacket 82 and the light
It is provided with pressure ring 85 between source 81, the light source 81 is fixed in the protective jacket 82, the top setting of the light source 81
There is the shield 86 abutted against with it, the top of the shield 86 is provided with the end cap 87 for closing the protective jacket 82, to protect
Shield light source 81 makes it be not easily susceptible to the influence of biology and sundries in ambient enviroment, guarantees the intensity and illumination duration of illumination.For
Guarantee sealing effect, is provided with sealing ring 88, the sealing ring 88 is preferably O-shaped between the end cap 87 and the sheath 86
Rubber seal, be not only convenient for purchasing, and sealing effect is good, reduce the equipment cost of bioreactor.In the present embodiment
In, the light source generator 8 is inserted into the tank body 1 by the top of the tank body 1, and is extended in the tank body 1
Lower part, so that each position of the tank body 1 can be by illumination.It is preferable to use LED light for the light source 81, both can guarantee photograph
Bright stability, and electric energy can be saved.The light source 81 can be radiation according to the use demand of cell line in the tank body 1
Light source or lighting source, or there is the electric light source of illumination and radiation function simultaneously.
As shown in Figure 1, the bioreactor further includes agitating device 6, the agitating device 6 includes by the tank body 1
The top column structure 61 and power part 52 that extend to the bottom of the tank body 1.The power part 62 and the column
The one end for being located at 1 top of tank body in body structure 61 is fixedly connected, and the power part 62 drives the column structure 61 certainly
Turn and/or swing, to be disturbed to the cell line in the tank body 1.At interval of predetermined length set on the column structure 61
Equipped with propeller blade 63, when 61 rotation of column structure, the propeller blade 63 is in the cylinder
Rotation under the drive of structure 61;When drive lower swing of the column structure 61 in the power part 62, the propeller
Formula stirring blade 63 is also swung while rotation.Preferably, the power part 62 includes motor, and the motor is preferred
For can either output torque can export the motor of amplitude again, drive the column structure rotation and vibration to realize simultaneously.Its
In, the shape of the column structure 61 does not limit, and can may be square column for cylinder, can also be Special-Shaped Column, with convenient
Installing and using is standard.The column structure 61 extends to the specific location of the bottom of the tank body 1 according to the tank body 1
Volume and height are configured, it is preferable that one end that the column structure 61 is located at the bottom of the tank body 1 is set to the tank
At the boundary position of the wall structure 12 of ontology 11 and the back taper, to guarantee the cell line of each position in the tank body 1
Disturbance can be experienced.The quantity of the propeller blade 63 should not be excessive, two adjacent propeller types
The distance between stirring blade 63 should not be too large, to prevent from generating unnecessary injury to cell line.Since stirring blade is spiral shell
The blade construction of rotary propeller type can effectively reduce the propeller blade 63 and reduce in its rotation and swing process
The mechanical wounding of the cell line of shearing force pair guarantees to cultivate efficiency and cultivates quality.Meanwhile passing through the propeller leaf
The rotation of piece 63 can also enhance the dissolved oxygen environment in the culture solution in the tank body 1, create better culture environment, turn out
The cell line of better quality.During the cultivation process, at interval of a period of time, the air sparger to inside the tank body 1 by
It is passed through gas upwards down, the cell line of 1 bottom of tank body becomes dispersity from coherent condition under the drive of air-flow, described
The dispersion effect of cell line is reinforced in the rotation of propeller blade 63.
Cell line is shifted for convenience, transfer outlet is provided on the wall structure 12 of the bottom of the tank body 1
(not shown), the transfer outlet are used to remove the cell line for being located at the bottom of the tank body 1 from the tank body 1,
And then it is transferred in bigger reactor.Inoculation entrance 13 is provided on the wall structure 12 at the top of the tank body 1, it is described
Inoculation entrance 13 is for being inoculated with the cell line into the tank body 1.
Further, outlet passageway structure 7, the outlet are provided on the wall structure 12 at the top of the tank body 1
Channel design 7 includes vertically extending condenser pipe 71.The outlet passageway structure 7 can will be in cell line growth growth course
The metabolism gas of generation is discharged inside the tank body 1, to guarantee the cleaning of environment in the tank body 1, obtains preferably culture
Breed environment.The condenser pipe 71 gas being discharged from the outlet passageway structure 7 can be carried out condensation and by condensed water from
It is discharged in the condenser pipe 71.
Embodiment 2
Difference between the present embodiment and embodiment 1 is, passes through welding side between the ontology and disk of air sparger
Formula or fastener are fixed together, without being fixed by fixed structure.
In addition, it is provided with filter device in the outlet passageway structure in the present embodiment, the filter device includes
Drying section and adsorption section, the drying section to from the steam in the gas being discharged in tank body for adsorbing, to guarantee to be discharged
Gas outside to the tank body be all it is dry, prevent the environment outside tank body excessively moist.The adsorption section be used for from
The harmful substance in gas being discharged in the tank body is adsorbed, to guarantee that the air being discharged to outside the tank body is all by net
Change, not can cause environmental pollution.
Embodiment 3
Difference between the present embodiment and embodiment 1 is, the entire length ratio of the light source generator in the present embodiment
The length of light source generator described in embodiment 1 is short, and specifically, the length of the light source generator in the present embodiment is about
The radial dimension of half of tank body.Light source generator in the present embodiment is uniformly arranged along the short transverse of the tank body, and
The light source generator is transversely arranged, with guarantee can in the entire short transverse and entire transverse direction of the tank body
It is hard to bear to be irradiated to uniform light source, guarantee the cultivation reliability of cell line.