CN117256479A

CN117256479A - Industrial automatic biological reaction device and control method thereof

Info

Publication number: CN117256479A
Application number: CN202311560558.0A
Authority: CN
Inventors: 刘冰; 刘金坤; 张洪喜; 张明臣; 高秀君
Original assignee: Shanghai Kedilian Technology Co ltd
Current assignee: Shanghai Kedilian Technology Co ltd
Priority date: 2023-11-22
Filing date: 2023-11-22
Publication date: 2023-12-22
Anticipated expiration: 2043-11-22
Also published as: CN117256479B

Abstract

The invention discloses an industrial automatic biological reaction device and a control method thereof, wherein the industrial automatic biological reaction device comprises a biological reactor, a gas distributor arranged at the bottom of the biological reactor and a shearing device arranged above the gas distributor; the gas distributor provides air with a certain pressure, so that the culture solution and plant tissues in the bioreactor can move along the rising and falling circulation of the track close to the paraboloid, and the culture solution and plant tissues are intermittently sheared by the shearing device and new callus tissues are provided in the falling process. When the gas distributor is used for providing gas for the inside of the bioreactor, the culture solution and the plant tissues are caused to generate circular motion close to a paraboloid, so that the contact area of the culture solution and the plant tissues with the gas is improved, the oxygen dissolution rate of the culture solution is improved, the plant tissues are prevented from being rotten and spoiled, and on the other hand, the plant tissues can be sheared by the shearing device, so that the winding is avoided, the discharging is facilitated, and the growth efficiency and quality of the plant tissues are improved as a whole.

Description

Industrial automatic biological reaction device and control method thereof

Technical Field

The invention belongs to the technical field of biological reaction devices, and particularly relates to an industrial automatic biological reaction device and a control method thereof.

Background

Along with the continuous improvement of living conditions, people generally pay more attention to strengthening physique through medicinal materials with nourishing effect by diet, and perform health care. Due to the huge population, the demand for medicinal plants has risen dramatically. In addition, the cultivation of medicinal plants by the traditional method not only requires a large amount of land and a long growth period, but also requires a proper climate. Any inappropriateness of conditions will limit and decrease the scale of cultivation of the medicinal plants and the yield of the medicinal plants.

Therefore, scientific workers develop a method and a culture device for large-scale cultivation by using isolated tissues or cells of plants. By combining the organs of plants: peeling roots, stems, leaves and the like, then placing the plant into a culture medium containing nutrient components for cultivation, simultaneously providing other environmental conditions such as temperature, illumination and the like suitable for growth, inducing organs of the plant into callus, adventitious buds and adventitious roots, finally, using the callus, the adventitious buds and the adventitious roots as seeds for cultivating the plant, and placing the plant into a cultivation device for cultivation. Furthermore, the defect that the cultivation of the medicinal plants depends on the conditions of land, climate and the like is overcome by culturing the adventitious roots through the culture device, and the yield of the medicinal plants can be improved by large-scale cultivation through a large number of culture devices.

In the prior art, before the seed is cultivated by the cultivation device, the cultivation device and the cultivation liquid are required to be sterilized, and gas required by cultivation is required to be provided in the cultivation device in the cultivation process, so that the quality of seed cultivation can be seriously affected when the sterilization efficiency is poor or the gas is insufficient.

When plant tissues are adventitious roots, the growth speed of the adventitious roots in a culture device is much faster than that of original roots, a large amount of adventitious roots can be intertwined with each other after a period of time, even are wound and agglomerated, the production speed and quality of the adventitious roots can be seriously influenced, and the discharge can be unsmooth.

The present invention has been made in view of this.

Disclosure of Invention

The invention aims to solve the technical problems of overcoming the defects of the prior art, and provides an industrial automatic biological reaction device, when a gas distributor is used for providing gas for the inside of a biological reactor, a culture solution and plant tissues are caused to generate a circular motion close to a paraboloid, so that on one hand, the contact area of the culture solution and the plant tissues with the gas is improved, the dissolved oxygen rate of the culture solution is improved, the plant tissues are prevented from being rotten and spoiled, on the other hand, the plant tissues can be sheared by a shearing device, the winding is avoided, the discharging is facilitated, and the growth efficiency and the quality of the plant tissues are improved as a whole.

In order to solve the technical problems, the invention adopts the basic conception of the technical scheme that:

an industrial automation bioreactor device comprises a bioreactor with a conical bottom wall, wherein a gas distributor and a shearing device arranged above the gas distributor are arranged on the bottom wall;

one end of the aeration part of the gas distributor is provided with a distance from the bottom wall, the other end of the aeration part is tangential to or intersects with the central line of the bioreactor, the gas provided by the aeration part enables the culture solution and plant tissues in the bioreactor to ascend along the area close to the central line and then spread to the peripheral side and circularly move along the track close to the outer paraboloid of the inner wall of the bioreactor, and the culture solution exerts certain force on the plant tissues in the ascending and descending processes along the track close to the paraboloid to enable the plant tissues to be basically arranged and flow in the length direction in a regular manner along the track close to the paraboloid;

the shearing device is vertically arranged on the bottom wall and comprises a rotatable cutter, a shearing plane where the cutter is located and a track which is close to the falling of the paraboloid form are acute angles, and the culture solution guides plant tissues which are regularly arranged into the cutter in the falling process along the track which is close to the paraboloid form.

Further, the cutter comprises double cutters which are distributed up and down, and most plant tissues are sheared into the size of the interval between the double cutters.

Further, the double-blade comprises a first blade and a second blade attached to the first blade, and the second blade rotates relative to the first blade to form a continuously opened and closed notch.

Further, the ends of the first blade and the second blade are respectively bent toward the side where the incision is formed, so that an approximately annular incision is formed.

Further, the ends of the second blades which are distributed up and down are connected through a frame body which is coated on the outer side of the first blade.

Further, the gas distributor is alternatively connected with the steam supply main pipe and the gas supply main pipe, and is used for introducing high-temperature steam for sterilization into the seed tank in the early stage of culture or introducing gas for culture into the seed tank in the culture process.

Further, the aeration portion of the gas distributor provides micro-bubbles into the culture solution of the bioreactor.

Further, the aeration part is provided with an extension length and is in a long column shape, the inside of the aeration part is hollow for air inlet, and the cavity wall is fully distributed with micropores for air outlet.

Further, the method further comprises the following steps:

the support frame is provided with functional pipelines, and the functional pipelines at least comprise a steam supply main pipe and a gas supply main pipe;

At least two bioreactors connected to each other, the bioreactors being disposed on one side of the support frame;

the gas distributor is arranged on the bioreactor and is alternatively connected with the steam supply main pipe and the gas supply main pipe, and high-temperature steam for sterilization or gas for culture is respectively introduced into the bioreactor.

Further, at least two gas distributors are symmetrically arranged on the conical bottom wall;

the gas inlet part of the gas distributor is connected with the same gas inlet pipeline, and the gas inlet pipeline is communicated with one of the steam supply main pipe and the gas supply main pipe.

Further, the air inlet pipeline is respectively connected with the steam supply main pipe and the air supply main pipe through three-way valves.

Further, the shearing device and the gas distributor are distributed on the bottom wall at intervals;

the shearing device has a gap between the ends extending toward the centerline of the bioreactor.

Further, the two bioreactors connected with each other comprise a seed tank and a culture tank, and the seed tank and the culture tank are connected through at least one seed transferring pipeline and at least one seed returning pipeline;

the seed transfer pipeline is used for transferring the culture solution containing plant tissues in the seed tank into the culture tank;

The seed returning pipeline is used for returning the part of the culture solution containing the plant tissues after dilution in the culture tank to the seed tank.

Further, the seed transfer pipeline stretches into the one end of cultivateing the jar is equipped with the discharge part, the seed return pipeline stretches into the one end of seed jar is equipped with feed back portion, discharge part and feed back portion are tubular structure, have an extension length, set up to the focus direction slope of jar body respectively.

A control method of an industrial automation biological reaction device, applied to the industrial automation biological reaction device, the method comprising:

the gas distributor is controlled to be opened, and air with certain pressure is provided for the bioreactor, so that the culture solution and plant tissues in the bioreactor can move up and down circularly along the track approaching to the paraboloid;

the cutting device is controlled to be opened intermittently, and new callus is provided by cutting plant tissues in the descending process.

Further, the method comprises the steps of: and controlling adjacent second blades connected by a frame body outside the first blades in the shearing device to rotate relative to the first blades which are respectively arranged in a pasting way, so that the incisions formed by the relative rotation of the first blades and the second blades are opened and closed simultaneously, and the plant tissues in the descending process are sheared into fixed-length small sections.

After the technical scheme is adopted, compared with the prior art, the invention has the following beneficial effects:

1. the invention provides an industrial automatic biological reaction device, which selectively opens and closes a passage between a gas distributor and a steam supply main pipe and a passage between the gas distributor and a gas supply main pipe through a three-way valve, so that high-temperature steam for sterilization or gas for cultivation can be provided for the inside of a biological reactor by using the gas distributor, and micro bubbles are provided for a culture solution of the biological reactor by an aeration part of the gas distributor, so that the contact area between the gas and the culture solution and the dissolution rate of the gas in the culture solution are improved, and the sterilization efficiency and the quality of plant tissues are improved; in addition, a large number of small bubbles enter the culture solution, so that the flow of plant tissues in the culture solution is promoted, and the plant tissues are prevented from being damaged due to the fact that the plant tissues are not contacted with gas; the high-temperature steam or gas can be supplied by using the same gas distributor, so that the number of the gas distributors is reduced, and the cost is reduced.

2. According to the invention, the shearing device and the gas distributor are arranged on the bottom wall at intervals, and the relative positions of the shearing device and the gas distributor are reasonably arranged, so that the gas distributor provides air with certain pressure for the inside of the bioreactor, the culture solution and plant tissues in the bioreactor ascend and descend along the track close to the paraboloid and circularly move, the culture solution and plant tissues are intermittently sheared by the shearing device and new callus tissues are provided in the descending process, the plant tissues are prevented from being agglomerated and wound together, smooth discharging is ensured, and meanwhile, the newly added callus tissues can improve the growth efficiency of the plant tissues.

3. According to the invention, by optimizing the setting positions of the shearing device and the gas distributor, the shearing plane where the cutter of the shearing device is positioned and the track approaching the paraboloid type descent form an acute angle, the culture solution guides the plant tissues which are regularly arranged into the cutter in the process of descending along the track approaching the paraboloid type descent, the plant tissues which are regularly arranged in the process of descending are sheared into small sections with shearing surfaces being inclined planes by the cutter, the surface areas of the callus tissues are increased by the shearing surfaces being inclined planes, more plant tissues can grow, the production efficiency is improved, the shearing uniformity is high, the wound plant tissues are prevented from being sheared unevenly or repeatedly sheared, and the shearing into uniform small sections is convenient for uniformly controlling the production degree, so that the discharge is facilitated, and the wound plant tissues are prevented from blocking the pipeline.

4. In the invention, the seed tank and the culture tank are connected through at least one seed shifting pipeline and a seed returning pipeline, and continuous culture is realized through seed shifting; the part of the culture solution containing plant tissues after dilution in the culture tank is returned to the seed tank by the seed return pipeline, new seeds are provided for the seed tank again, other equipment is not needed in the whole process, and the whole process is not contacted with the external environment, so that the probability of bacteria contamination is reduced, the success rate of seed culture is improved, meanwhile, the use of equipment is reduced, the cost is reduced, and the operation is convenient.

The following describes the embodiments of the present invention in further detail with reference to the accompanying drawings.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiments of the invention and together with the description serve to explain the invention, without limitation to the invention. It is evident that the drawings in the following description are only examples, from which other drawings can be obtained by a person skilled in the art without the inventive effort. In the drawings:

FIG. 1 is a side view of the gas distributor and shearing apparatus of the present invention on the bottom wall of a bioreactor;

FIG. 2 is a top view of the distribution structure of the gas distributor and shearing device on the bottom wall of the seed tank of the present invention;

FIG. 3 is a top view showing the distribution structure of the gas distributor and the shearing apparatus on the bottom wall of the culture tank according to the present invention;

FIG. 4 is a schematic view of the structure of a gas distributor according to the present invention;

FIG. 5 is a schematic cross-sectional view of a gas distributor according to the present invention;

FIG. 6 is a schematic view of the gas distributor of the present invention disposed on the bottom wall of a bioreactor;

FIG. 7 is a schematic view of the structure of the shearing device of the present invention disposed on the bottom wall of the bioreactor;

FIG. 8 is a schematic view of the structure of the shearing device in the present invention;

FIG. 9 is a schematic view of the structure of a shearing section of the shearing device in the present invention;

FIG. 10 is a schematic view of a functional circuit according to the present invention;

FIG. 11 is a schematic view of another construction of the functional pipeline according to the present invention;

FIG. 12 is a schematic view of another construction of the functional pipeline according to the present invention;

FIG. 13 is a schematic view of the connection of a gas distributor to a gas inlet line in accordance with the present invention;

FIG. 14 is a schematic structural view of a bioreactor apparatus according to the present invention;

FIG. 15 is a schematic view showing the structure of a culture tank according to the present invention;

FIG. 16 is an enlarged schematic view of the structure at A in FIG. 15;

FIG. 17 is a schematic view of the structure of the seed tank of the present invention;

FIG. 18 is an enlarged schematic view of the structure at B in FIG. 17;

FIG. 19 is a schematic view showing the structure of a five-way valve at the bottom of the bioreactor of the present invention;

FIG. 20 is a schematic view showing the structure of a feeding device on a bioreactor according to the present invention;

FIG. 21 is a schematic view of the feeding device of FIG. 20;

FIG. 22 is a schematic diagram showing the structure of the detecting device on the bioreactor according to the present invention.

In the figure: 100. a seed tank; 200. a culture tank; 11. a bottom wall; 1. a main pipeline; 2. dividing pipelines; 4. a pneumatic valve; 5. a manual valve; 6. an input tube; f1, a cold row pipeline; f2, a heat exhaust pipeline; 410. a depressurization filter; 420. a discharge branch; a100, a main air supply pipe; b100, a steam supply main pipe; c100, a chilled water recovery main pipe; d100, a cooling water recovery main pipe; e100, a hot water recovery main pipe; c200, a chilled water supply main pipe; e200, a hot water supply main pipe; d200, a cooling water supply main pipe; c20, chilled water supply branch pipe; d20, cooling water supply branch pipes; e20, hot water supply branch pipes; c10, chilled water recycling branch pipes; d10, cooling water recycling branch pipes; e10, hot water recovery branch pipes; a110, an air inlet branch; a120, a check valve; a130, a filtering device; a140, filtering and eliminating branches; a150, a tank elimination branch; a160, a standby branch; a170, an exhaust pipeline; a180, a gas filter; a1, a supply unit; a2, a conveying unit; 9. a temperature control layer; a3, a temperature-regulating water tank; a301, a water containing cavity; a21, a water inlet pipe; a211, a water pump; a212, branch pipes; a22, a water outlet pipe; a23, a heat exchange structure; a24, a liquid inlet pipe; a241, chilled water inlet pipe; a242, a steam liquid inlet pipe; a25, a liquid outlet pipe; a251, freezing the water outlet pipe; a252, a steam liquid outlet pipe; b8, a three-way pipe; a1107, branch; 40. a gas distributor; 41. an air inlet part; 42. an aeration section; 43. a conduit; 431. an air inlet section; 432. an air outlet section; 433. a transition section; 44. a seat plate; 45. quick release joint; 451. sealing grooves; 52. stacking the pressing piece; 521. a through hole; 53. a hub kit; 531. a support ring; 532. a groove; 533. a connection hole; 54. a seal ring; 3. a shearing device; 90. a tank cavity; 31. a spacer bush; 311. a convex column; 312. a flange edge; 32. a shearing part; 321. a pin shaft; 322. a blade set; 323. a first blade; 324. a second blade; 325. a rotating end; 326. blade ends; 327. closing the side edges; 328. unfolding the side edges; 33. a transmission part; 331. a discharging section; 332. a drive section; 333. a discharge port; 34. a power section; 35. a guide cover; 351. an inlet; 352. a mounting port; 353. an outlet; 110. a tank bottom valve; 120. a connection port; 210. a maintenance port; 220. a water inlet; 230. an inoculation port; 300. a seed transferring pipeline; 310. a seed moving valve; 320. a five-way valve; 330. a discharging part; 400. CIP cleaning the pipeline; 500. a steam line; 1000. washing the pipeline; 600. a discharge pipe; 610. a discharge valve; 700. a sewage drain pipe; 710. a blow-down valve; 800. CIP discharge pipe; 810. CIP discharge valve; 900. a seed returning pipeline; 910. a seed returning valve; 920. a material returning part; 7. a material supplementing device; 13. a material supplementing structure; 14. acid supplementing structure; 15. an alkali supplementing structure; 16. a defoaming liquid supplementing structure; 113. a feed supplement pneumatic valve; 114. acid supplementing pneumatic valve; 115. an alkali supplementing pneumatic valve; 116. a defoaming liquid pneumatic valve is supplemented; 131. a material supplementing air inlet pipe; 132. a material supplementing air outlet pipe; 141. an acid supplementing air inlet pipe; 142. an acid supplementing air outlet pipe; 151. an alkali supplementing air inlet pipe; 152. an alkali supplementing air outlet pipe; 161. an air inlet pipe for supplementing defoaming liquid; 162. an air outlet pipe for supplementing defoaming liquid; 133. a feed supplementing air inlet valve; 134. a material supplementing exhaust valve; 143. an acid supplementing air inlet valve; 144. acid supplementing exhaust valve; 153. alkali supplementing air inlet valve; 154. alkali supplementing exhaust valve; 163. an air inlet valve for supplementing defoaming liquid; 164. an exhaust valve for supplementing defoaming liquid; 130. supplementing a feed box; 140. acid supplementing box; 150. an alkali supplementing box; 160. a defoaming liquid box is filled; 105. a feed supplement hose; 106. acid supplementing hose; 107. an alkali supplementing hose; 108. a defoaming liquid supplementing hose; 8. and a detection device.

It should be noted that these drawings and the written description are not intended to limit the scope of the inventive concept in any way, but to illustrate the inventive concept to those skilled in the art by referring to the specific embodiments.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions in the embodiments will be clearly and completely described with reference to the accompanying drawings in the embodiments of the present invention, and the following embodiments are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

In the description of the present invention, it should be noted that the positional or positional relationship indicated by the terms such as "upper", "lower", "inner", "outer", "front", "rear", etc. are based on the positional or positional relationship shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the apparatus or element in question must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention.

In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "connected," "abutting," and the like are to be construed broadly, and may be, for example, detachably connected, mechanically connected, or electrically connected; can be directly connected or indirectly connected through an intermediate medium. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.

As one embodiment, the present invention provides an industrial automation bioreactor apparatus, as shown in fig. 11, comprising at least two bioreactors, including a seed tank 100 and a culture tank 200.

The culture tank 200 is used for rapidly culturing and proliferating plant tissues to realize quantitative production of the plant tissues; the seed pot 100 is used for inoculating plant tissue as seeds for rapid cultivation of proliferating plant tissue in the next cycle of the culture pot 200.

The plant tissue may be in vitro plant tissue or cells such as adventitious roots, adventitious buds, etc., and in this embodiment, the adventitious roots of the cultured ginseng are mainly used.

The volume of the seed tank 100 is smaller than that of the culture tank 200, and is 1/3 to 1/5 of that of the culture tank 200.

As shown in fig. 1, the bioreactor further comprises a gas distributor 40 arranged at the bottom of the bioreactor and a shearing device 3 arranged above the gas distributor 40.

The gas distributor 40 supplies air with a certain pressure to make the culture solution and plant tissue in the bioreactor move up and down circularly along the track approaching to the paraboloid, and the culture solution and plant tissue are cut intermittently by the cutting device 3 during the descending process to provide new callus.

In the present embodiment, when the gas distributor 40 is used to supply gas into the bioreactor, the culture solution and plant tissue are caused to produce a circular motion close to a paraboloid, so that on one hand, the contact area between the culture solution and plant tissue and the gas is increased, the oxygen dissolution rate of the culture solution is increased, and the plant tissue is prevented from being rotten and spoiled; on the other hand, the shearing device 3 can be used for shearing the plant tissues, so that the winding is avoided, and the discharging is convenient; and the newly added callus can improve the growth efficiency of plant tissues, and the growth efficiency and quality of the plant tissues are improved as a whole.

When the plant tissue in the bioreactor is cultivated to a certain degree or for a certain time, the shearing device 3 is controlled to be opened, the plant tissue with a certain length is sheared into small sections, the plant tissue is prevented from being wound to be fully contacted with gas to be rotten and deteriorated, and the specific shearing times are determined according to the cultivation condition of the plant tissue; when the plant tissue culture is completed and the transplanting or discharging is needed, the shearing device 3 is controlled to be opened to shear the plant tissue, so that the discharging is smooth.

The bioreactor has a conical bottom wall 11, and the gas distributor 40 is arranged on the bottom wall 11, and the parabolic track is an inner parabolic surface rising along the inner wall of the bioreactor and falling near the central axis area, or an outer parabolic surface rising near the central axis area and falling along the inner wall of the bioreactor.

One end of the aeration portion 42 of the gas distributor 40 is spaced from the bottom wall 11, and the other end is tangential to or intersects with the center line of the bioreactor, so that the gas supplied from the aeration portion 42 forms an outer paraboloid in the bioreactor rising near the center line and falling near the inner wall of the bioreactor.

For example, as shown in fig. 2, at least two gas distributors 40 and two shearing devices 3 are provided on the bottom wall 11 of the seed tank 100. The shearing device 3 and the gas distributor 40 are alternately spaced on the bottom wall 11, and the shearing device 3 is installed at a position vertically higher than the gas distributor 40.

Preferably, the line connecting the mounting positions of the two shearing devices 3 and the line connecting the mounting positions of the two gas distributors 40 pass through the center of the seed tank 100, respectively.

The aeration sections 42 of the two gas distributors 40 according to the invention are preferably arranged on the same plane when mounted and the projections of the extension directions in the seed tank 100 are approximately parallel, symmetrically arranged with respect to the center of the bioreactor.

As shown in fig. 2 and 6, the aeration section 42 has an extension length, one end connected to the conduit 43 is spaced from the bottom wall 11, and the other end extends in a direction approaching the center of the bioreactor while being tangent or intersecting at the center line of the bioreactor. The distance between the end of the aeration portion 42 connected with the conduit 43 and the bottom wall 11 is smaller than the distance between the other end of the aeration portion 42 and the bottom wall 11. That is, the length of the conduit 43 extending into the tank is short, so that the aeration portion 42 can be mounted on the bottom wall 11 and the mounting angle can be adjusted.

The gas supplied from the aeration unit 42 has a certain pressure, and the culture solution and plant tissue in the bioreactor are circulated along the outer parabolic path which is raised near the central axis and then spread to the peripheral side, and lowered near the inner wall of the bioreactor, and the culture solution is applied with a certain force to the plant tissue in the process of raising and lowering along the path near the parabolic path so as to make the plant tissue basically arranged and flowed in a regular manner along the length direction along the parabolic path.

The shearing device 3 has a gap between the ends extending toward the central line of the bioreactor, and the gap is used for passing the culture solution and plant tissues rising near the central line.

Plant tissue arranged in the same direction under the action of the parabolic air flow in the bioreactor flows downwards into the incision of the shearing device 3.

The shearing device 3 and the gas distributor 40 adopt the arrangement mode, the uniformly distributed gas can be provided in the bioreactor, and the distribution ratio of the aeration part 42 at the central line of the bioreactor is higher than that at the bottom wall 11, so that the kinetic energy provided by the gas at the central line area is higher than the potential energy and the upper pressure of the culture solution, the culture solution can move upwards under the driving of the air flow, when the culture solution moves to a certain height, the kinetic energy generated by the gas on the culture solution is far away from the aeration part 42 and is weakened and smaller than the potential energy and the upper pressure of the culture solution, and meanwhile, the potential energy and the upper pressure of the culture solution at the inner wall are higher than the kinetic energy provided by the gas, so that the culture solution can circularly move along the track of the outer paraboloid which is upwards and downwards near the inner wall of the bioreactor along the central line area, and the descending track is close to the vertical descending due to the gravity of the culture solution, on the one hand, the contact area of the gas, the culture solution and plant tissue is further improved, and the plant tissue is prevented from rotting due to the fact that the stirring device is not required to be arranged too much; on the other hand, the culture solution generates driving force to plant tissues in the ascending and descending processes along the track approaching to the paraboloid, so that the plant tissues are regularly arranged towards the same direction, and the plant tissues are prevented from being wound together in the growing process, and the growing efficiency is prevented from being influenced; and the moving culture solution guides the plant tissues to be regularly arranged and flow into the incision of the shearing device 3, and simultaneously, the cutters in the shearing device 3 rotate to generate vortex suction force, so that the regularly arranged plant tissues are further sucked into the incision of the shearing device 3, the shearing uniformity is ensured, and the wound plant tissues are prevented from being sheared unevenly or repeatedly sheared.

Further, the included angle β between the shearing device 3 and the bottom wall 11 is adjustable, the aeration portion 42 and the shearing device 3 extend and intersect at a point along the respective length direction to form an included angle β1, the included angle between the bottom wall 11 and the horizontal plane is β2, and the included angle β1+β2=β is satisfied, and preferably, when the included angle β is 90 degrees, the air inlet effect of the aeration portion 42 is optimal.

Also, as shown in fig. 3 and 6, at least four gas distributors 40 and two shearing devices 3 are provided on the bottom wall 11 of the culture tank 200 in a similar manner to the seed tank 100.

The four gas distributors 40 are uniformly arranged in the circumferential direction of the bottom wall 11 and are arranged at equal intervals. The aeration portion 42 of the gas distributor 40 is maintained horizontal and has a certain length. One end of any two opposite groups of aeration parts 42 connected with the guide pipe 43 is spaced from the bottom wall 11, the other end of the aeration parts is tangential or intersected at the central line of the bioreactor, and the projections in the culture tank 200 are approximately parallel and symmetrically arranged relative to the center of the bioreactor; the projections of two adjacent sets of aeration sections 42 in the culture tank 200 are approximately vertical.

The two gas distributors 40 are provided with a shearing device 3 at intervals, and the connecting line of the installation position of one shearing device 3 on the same side of the bottom wall 11 and the installation positions of two adjacent gas distributors 40 in the culture tank 200 approximately forms an isosceles triangle, and the installation position of the shearing device 3 is higher than the gas distributors 40 in the vertical direction.

By reasonably setting the relative positions of the shearing device 3 and the gas distributor 40, the gas provided by the aeration part 42 of the gas distributor 40 promotes the culture solution and plant tissues in the bioreactor to rise along the area close to the central axis, spread to the peripheral side and circularly move along the track of the outer paraboloid close to the inner wall of the bioreactor, and the culture solution exerts certain force on the plant tissues in the rising and falling processes along the track close to the paraboloid so as to enable the plant tissues to be basically arranged and flow in a regular way along the length direction.

Alternatively, the aeration section 42 of the gas distributor 40 is disposed adjacent to the bottom wall 11, and the gas supplied from the aeration section 42 causes the culture solution and plant tissue in the bioreactor to circulate along the track of the inner paraboloid which is raised near the inner wall of the bioreactor and lowered near the central axis region.

In the process of ascending and descending along the track approaching to the paraboloid, the culture solution exerts certain force on plant tissues to enable the plant tissues to basically and regularly arrange and flow along the length direction along the track approaching to the paraboloid, so that the plant tissues are conveniently sheared into small sections by the shearing device 3, and the plant tissues are prevented from being repeatedly sheared together.

The shearing device 3 comprises a rotatable cutter into which the culture medium is guided in a regular array of plant tissue during descent along a trajectory approaching a parabolic shape.

The cutter comprises double cutters which are distributed up and down, and most plant tissues are sheared into the size of the interval between the double cutters.

An included angle is formed between the shearing device 3 and the bottom wall 11, and an included angle is formed between the shearing plane where the double knife is positioned and the moving track of the plant tissue along the parabolic descending.

In this embodiment, the included angle between the shearing device 3 and the bottom wall 11 is preferably 90 °, that is, the shearing device 3 is vertically disposed on the bottom wall 11, at this time, the shearing plane where the double knives are located and the track where the paraboloid type descends are acute angles, the culture solution guides the regularly arranged plant tissues into the cutters in the descending process along the track which is close to the paraboloid type, so, when the plant tissues are sheared into small sections by the shearing device 3 by using the cutters, the shearing surface is an inclined plane, the area of the shearing surface is increased, seeds of more plant tissues such as ginseng adventitious roots can be cultivated, the production efficiency is improved, the shearing uniformity is high, the wound plant tissues are prevented from being sheared unevenly or repeatedly, the production degree of the wound plant tissues is conveniently controlled uniformly by shearing into uniform small sections, the discharging is also convenient, and the wound plant tissues are prevented from blocking the pipeline.

Or, the installation angle of the shearing device 3 is adjusted, so that the shearing plane where the double knife is positioned is approximately perpendicular to the paraboloid-shaped descending track, plant tissues can be opposite to the incision of the shearing device 3, the number of the plant tissues entering the shearing device 3 each time is increased, the shearing efficiency is improved, and the plant tissues are ensured to be sheared into small sections with approximate length and uniformity.

In another embodiment, a gas distributor 40 is described, and as shown in fig. 4 to 6 and 12, the gas distributor 40 includes a gas inlet portion 41, an aeration portion 42, and a conduit 43 connecting the gas inlet portion 41 and the aeration portion 42, and the gas inlet portion 41 and the aeration portion 42 are respectively connected to both ends of the conduit 43.

The air inlet portion 41 is provided on the bottom wall 11 in a penetrating manner, is in communication with the main steam supply pipe B100 and the main air supply pipe a100, and is used for introducing high-temperature steam for sterilization into the seed tank 100 in the early stage of cultivation and introducing gas for cultivation into the seed tank 100 during cultivation.

In this embodiment, the high temperature steam for sterilization and the gas for culture can be supplied into the bioreactor through the gas distributor 40, so that the gas distributor is multipurpose, and the inside of the gas distributor 40 can be cleaned and sterilized when the high temperature steam for sterilization is supplied, thereby solving the problem that the inside of the traditional gas supply part is difficult to clean.

The center of the aeration part 42 is provided with a cavity, the cavity is communicated with the air inlet part 41 and the guide pipe 43, and the cavity wall of the cavity is provided with micropores communicated from the outer side of the aeration part 42 into the cavity, and a large number of micropores are densely and uniformly distributed on the whole cavity wall. The check valve a120 is located outside the bottom wall 11 and connected to the air intake portion 41.

Preferably, the aeration portion 42 is made of a titanium alloy material such that plant tissue cannot adhere to the outer surface of the aeration portion 42, thereby avoiding the plant tissue from accumulating and spoiling.

In this embodiment, a large number of micropores are densely formed on the side wall of the aeration portion 42, and all the micropores are communicated with a cavity formed in the center of the aeration portion 42, so that gas entering the cavity of the aeration portion 42 through the conduit 43 can be sprayed into the culture solution from all the micropores at the same time, thereby forming a large number of small bubbles in the culture solution at the same time, and a large number of continuous small bubbles move outwards and float upwards from the outer surface of the aeration portion 42, so that the small bubbles are prevented from being polymerized into large bubbles, the driving force of circulating flow is generated on the culture solution and plant tissues in the tank body, the contact and dissolution rate of the gas and the culture solution are improved, and the circulating flow of the culture solution is further improved by the floating movement of a large number of small bubbles in the dispersion of the culture solution.

Preferably, the aeration portion 42 is formed by stacking a large number of small particles of a metal material having a diameter of less than 1mm and then sintering them together to form a housing having a cavity in the center.

The small particles are spherical, a large number of small particles are close to adhesion, and gaps are reserved between adjacent small particles. The gaps are sequentially arranged and connected in the radial direction of the aeration portion 42 to form micropores extending from the inner surface of the chamber wall to the outer surface of the chamber wall, and thus, the outer surface of the aeration portion 42 is covered with micropores, like the meshes of a screen mesh.

The aeration portion 42 has an extended length, and is in a long column shape, one end of the aeration portion 42 is connected with the conduit 43, the central axis of the aeration portion 42 coincides with the central axis of the end of the conduit 43 and extends in a direction away from the conduit 43 along a straight line, and meanwhile, the conduit 43 is in a long straight shape, so that the aeration portion 42 coincides with the central axis of the conduit 43.

The gas distributor 40 further comprises a seat plate 44, said seat plate 44 being connected to said duct 43 and extending radially outwards of the duct 43, forming a shape in which the duct 43 passes perpendicularly through the centre of the seat plate 44. In particular, the seat plate 44 is located between both ends of the duct 43, the aeration portions 42 are distributed on one side of the seat plate 44, and the air intake portion 41 is disposed on the other side of the seat plate 44.

Preferably, the seat plate 44 is provided in a circular flat plate shape so that the gas distributor 40 can be rotated around the guide duct 43 when installed.

Conduit 43 includes an elongated straight intake section 431. The air inlet sections 431 are respectively suspended to at least one length toward both sides of the seat plate 44, thereby facilitating the better connection and fixation of the aeration section 42 and the air inlet section 41 to the guide duct 43.

The duct 43 further includes an air outlet section 432, and one end of the air outlet section 432 is connected to the air inlet section 431, and the other end is connected to the aeration section 42. Preferably, the angle between the outlet section 432 and the inlet section 431 is greater than 90 °.

The duct 43 is further provided with a transition 433 connected between the inlet section 431 and the outlet section 432, the transition 433 being parallel to the seat plate 44.

In this embodiment, the optimizing conduit 43 includes a plurality of straight sections and the adjacent straight sections are connected in a bending manner, so that when the gas distributor 40 is installed in the bioreactor, the aeration portion 42 is ensured to be located at more positions of the bioreactor by rotating the gas distributor 40, so that the hoverable position range of the aeration portion 42 in the bioreactor is enlarged, and bubbles can be fully diffused into the culture solution from the periphery of the aeration portion 42.

Further, the air inlet 41 includes a quick release connector 45, the quick release connector 45 is conical and is provided at the end of the duct 43, and the conical bottom surface of the quick release connector 45 coincides with the air inlet end surface of the duct 43. The inlet of the duct 43 is located in the center of the bottom surface. The tapered surface of the quick release connector 45 is directed toward the seat plate 44 and gradually contracted to the outer peripheral surface of the guide tube 43.

The conical bottom surface of the quick release coupling 45 is provided with a seal groove 451, the seal groove 451 is recessed from the bottom surface toward the inside of the quick release coupling 45 and a recess is formed in the bottom surface, and the seal groove 451 surrounds the outer periphery of the air inlet of the guide pipe 43.

The bottom wall 11 is provided with an interface for installing the gas distributor 40, the aeration part 42 is positioned inside the bioreactor, and the gas inlet part 41 is suspended outside the bioreactor.

Preferably, the aeration section 42 is horizontally disposed inside the bioreactor.

And a ring-shaped lamination piece 52, wherein the lamination piece 52 is sleeved with the seat plate 44 and is abutted with the plate surface of the seat plate 44 facing the outer side of the bioreactor by the edge of the annular hole of the lamination piece, so as to clamp the gas distributor 40 on the bottom wall 11.

Also included is a hub sleeve 53 nested in the interface, the outer peripheral surface of which is connected to the interface and projects inward and outward of the bottom wall 11 in the axial direction thereof, and the inner peripheral surface of which is connected to the seat plate 44.

The hub unit 53 has a support ring 531 provided at one end in the bottom wall 11, and extending radially from the inner peripheral surface of the hub unit 53 to the center, and the seat plate 44 is fitted over the inner peripheral surface of the hub unit 53 from the outside of the bottom wall 11 and abuts against the support ring 531.

And a sealing ring 54 which is arranged between the seat plate 44 and the supporting ring 531 and is clamped by the end surface of the seat plate 44 facing the bioreactor and the supporting ring 531.

The support ring 531 is provided with a groove 532 opening out of the bottom wall 11, and the sealing ring 54 is installed in the groove 532 by pressing the seat plate 44.

The end surface of the hub sleeve 53 protruding outside the bottom wall 11 is provided with a connecting hole 533 extending along the axial direction thereof, and the stacking member 52 may be connected with the end surface of the hub sleeve 53 to clamp the seat plate 44, and is provided with a through hole 521 corresponding to the connecting hole 533 for penetrating a fixing screw.

The inner peripheral surface of the hub sleeve 53 is a cylindrical surface, the seat plate 44 is spherical and has a radius equal to that of the inner peripheral surface of the hub sleeve 53, and the lamination piece 52 and the support ring 531 rotatably clamp the seat plate 44 in the hub sleeve 53.

One end of the stacking piece 52 and the supporting ring 531 near the inner circumferential surface of the hub unit 53 is provided with a spherically curved surface.

In this embodiment, the seat plate 44 is connected to the bottom wall 11 by the crimp 52 and the clamping of the hub kit 53, so that the gas distributor 40 penetrating and fixed from the center of the seat plate 44 has more positions in the bioreactor. When the gas distributor 40 is installed, the seat plate 44 can rotate by any angle relative to the hub sleeve 53, and the posture and the position of the aeration portion 42 can be changed by rotating the seat plate 44, so that the installation of the gas distributor 40 is greatly facilitated. Preferably, when the aeration portion 42 is parallel to the horizontal plane, the screws are tightened to fix the gas distributor 40 to the bottom wall 11.

In another embodiment, a shearing device 3 is described, in which when the plant tissue is adventitious roots of ginseng, the plant tissue grows much faster in the pot than the native roots, and a large amount of plant tissue grows over a period of time to intertwine and even form clusters, which seriously affects the production speed and quality of the plant tissue. Therefore, the plant tissue needs to be sheared so as to facilitate seed transfer and seed returning, accurately control the length of the plant tissue, ensure the growth speed and improve the yield and quality of the plant tissue.

As shown in fig. 7 to 9, a detachable shearing device 3 is provided on the bottom wall 11 of the tank body of the seed tank 100 and the culture tank 200, and the axial direction of the shearing device 3 forms an included angle with the bottom wall 11, preferably 90 °.

Taking the example in which the shearing apparatus 3 is provided on the culture tank 200:

the shearing device 3 comprises a spacer 31, a shearing part 32, a transmission part 33, a power part 34 and a guide cover 35.

The bottom wall 11 of the culture tank 200 is provided with a circular mounting hole, and the outer circumferential surface of the spacer 31 is cylindrical and has the same diameter as the mounting hole. The spacer 31 is detachably fitted into the mounting hole in the bottom wall 11.

One side of the spacer 31 is connected to the shear part 32, and the other side is connected to the power part 34, for separating the shear part 32 and the power part 34 from each other inside and outside the culture tank 200. The spacer 31 can be directly inserted into the shear part 32 through the mounting hole by making the cross section larger than that of the shear part 32, so that the shear device 3 can be simply and quickly arranged on the culture tank 200.

In this embodiment, the shearing device 3 capable of shearing plant tissues is disposed in the culture tank 200, and the spacer 31 is disposed between the shearing portion 32 and the power portion 34, so that the shearing device 3 is installed in and outside the bottom wall 11 of the culture tank 200 in a scattered manner, thereby not only controlling the length of the plant tissues by the shearing device 3 to maintain and promote the cultivation efficiency of the plant tissues, but also avoiding germs or impurities from entering the culture tank 200 through the shearing device 3, and improving the tightness of the culture tank 200.

The spacer 31 protrudes from the surface of the bottom wall 11 toward the tank cavity 90 along the vertical direction of the bottom wall 11 of the culture tank 200. The spacer 31 is formed with a concave groove on the other side corresponding to the position where the spacer 31 protrudes toward the tank cavity 90 as seen from the outside of the bottom wall 11 of the culture tank 200.

The shearing part 32 is connected with the outer convex surface of the spacer bush 31, and the power part 34 is arranged at the concave groove. Preferably, the shearing part 32 is provided with a member which is sleeved on the periphery of the spacer 31 and can rotate relative to the spacer 31, and the power part 34 attracts and drives the member from the other side of the spacer 31 through the spacer 31, so as to drive the shearing part 32 to shear plant tissues.

In this embodiment, by optimizing the shape of the spacer 31, the spacer 31 protrudes into the cavity 90 of the tank body, so as to enhance the attraction and driving effect of the power portion 34 on the shearing portion 32, ensure that the shearing device 3 shears plant tissue better, avoid plant tissue winding and agglomerating, and promote faster growth of plant tissue.

The spacer 31 may be provided with an annular groove recessed in the direction of the central axis thereof on the outer circumference, and the middle portion of the spacer 31 is located between the groove bottom and the groove top in the direction of the central axis of the annular groove.

Preferably, the middle part of spacer 31 is flush with the top of the tank, i.e., the middle part of spacer 31 is flush with the surface of bottom wall 11 of culture tank 200.

Specifically, when the spacer 31 is provided on the culture tank 200, the annular groove of the spacer 31 extends from the surface of the bottom wall 11 toward the tank body cavity 90 along the perpendicular direction of the bottom wall 11 of the culture tank 200, and the notch of the annular groove is located on the surface of the bottom wall 11 of the culture tank 200, so that the spacer 31 forms an annular structure having the annular groove.

Preferably, the middle part of the spacer 31 protrudes toward the tank cavity 90 to form a cylindrical structure with a cylindrical groove. In this embodiment, by optimizing the shape of the spacer 31, the spacer 31 protrudes into the cavity 90 of the tank body, so as to enhance the attraction and driving effect of the power portion 34 on the shearing portion 32, ensure that the shearing device 3 shears plant tissue better, avoid plant tissue winding and agglomerating, and promote faster growth of plant tissue.

The shearing part 32 comprises a first blade 323 and a second blade 324 which are respectively connected with the power part 34, the first blade 323 and the second blade 324 are arranged in a pasting way, and can be driven by the power part 34 to rotate oppositely or reversely to open and close so as to cut plant tissues.

The shearing portion 32 includes a pin 321 connected in series with the first blade 323 and the second blade 324, and the second blade 324 rotates relative to the first blade 323 with the pin 321 as a central axis, and forms a notch by the sides of the first blade 323 and the second blade 324.

The first blade 323 and the second blade 324 are elongated, the pin shaft 321 is vertically inserted in the centers of the first blade 323 and the second blade 324, and the second blade 324 rotates relative to the first blade 323 to form two symmetrical notches relative to the pin shaft 321.

In another connection manner, the shearing portion 32 includes a plurality of blade groups 322 connected in series to the pin 321, where each blade group 322 is formed by matching the first blade 323 and the second blade 324 in pairs and is distributed at equal intervals along the axis direction of the pin 321, so as to form a multi-layer structure. The spacing between adjacent blade sets 322 is between 1cm and 2cm, preferably between 1cm and 1.5 cm.

Specifically, all the first blades 323 in the blade set 322 are parallel to each other and are fixed with the pin shaft 321, all the second blades 324 are parallel to each other and are connected at the ends of the second blades 324 in the length direction of the second blades to form a frame body coated on the outer side of the first blades 323, and the second blades 324 rotate relative to the first blades 323 to form a continuously opened and closed notch.

In this way, all the second blades 324 in the blade groups 322 are connected together through the frame body, when the second blades 324 are driven to rotate relative to the first blades 323, the second blades 324 in the adjacent blade groups 322 rotate relative to the respective first blades 323 together to form adjacent continuously open and close cuts, and simultaneously, the plant tissues entering the cutting parts 32 are cut, so that the lengths of the cut plant tissues are consistent with the intervals between the adjacent blade groups 322.

In this embodiment, the shearing part 32 is provided with a plurality of groups of blades, and the blade groups 322 are arranged at fixed intervals, so that plant tissues with consistent lengths can be obtained by cutting the plant tissues, the plant tissues in the culture tank 200 have the same growth state, the nutrient content of the nutrient solution can be conveniently and regularly adjusted, the plant tissues can be trimmed, the nutrient solution circulation in the culture tank 200 is promoted, stable growth conditions are maintained, and the cultivation efficiency and the quality of the plant tissues are improved.

The second blade 324 includes a rotating end 325 and a blade end 326, one end of the blade end 326 is connected to the rotating end 325, and the other end extends in a diameter direction of the rotating end 325 and is suspended at an outer circumference to form a cantilever shape. The rotating end 325 is sleeved on the pin 321, and when the second blade 324 rotates relative to the first blade 323, the blade end 326 can rotate around the pin 321 to form a continuously opened and closed notch.

The first blade 323 has the same structure and shape as the second blade 324.

Wherein the blade end 326 includes a closed side 327 for coining and an open side 328 opposite the closed side 327. The closed side 327 and the open side 328 extend radially from the rotating end 325 to the other end and gradually converge toward each other.

The ends of the closed side 327 are located on the same diameter line, and the closed side 327 extends from the rotational end 325 to the other end along an arc. Finally, the closed side 327 of the blade end 326 is formed with an inwardly concave recess.

The slit gradually closes from both ends of the closing side 327 toward the notch when the second blade 324 rotates relative to the first blade 323.

Further, the second blade 324 includes a plurality of the blade ends 326 evenly distributed along the outer circumference of the rotating end 325. For example, three blade ends 326 are provided at intervals of 120 ° at the outer periphery of the rotating end 325; four blade ends 326 are provided at the outer circumference of the rotating end 325 at 90 intervals.

Preferably, the second blade 324 is provided with two blade ends 326 symmetrically distributed about the rotational end 325.

In particular, the deployment side 328 extends along an arc from the rotational end 325 to the outer periphery, and the closure side 327 also extends along an arc from the rotational end 325 to the outer periphery. And, the open side 328 and the closed side 327 gradually converge at a point.

The second blade 324 is S-shaped and is formed of two blade ends 326 connected to a rotating end 325 and disposed at 180 ° intervals.

The blade end 326 is curved upwardly in a circumferential direction from the closed side 327 to the open side 328.

Preferably, the blade end 326 is configured as a spiral surface along the diameter of the rotating end 325.

Preferably, the first blade 323 has an elongated shape, and the first blade 323 is provided at both ends with a sharp corner inclined to the diameter thereof, and a side of the sharp corner opposite to the closed side 327 is recessed toward the first blade 323.

The central axis of the power part 34 coincides with the central axis of the shearing part 32, and the shearing part 32 is driven to shear plant tissues.

The power unit 34 is provided with a rotor that magnetically drives the shear unit 32 to open and close the slit through the bottom wall 11. Specifically, a magnetic attraction piece made of a magnetic material is fixedly arranged on the rotor. The magnetic attraction member may attract the cutout portion 32 via the bottom wall 11 and follow the rotation of the rotor around the central axis of the power portion 34.

In this embodiment, the power portion 34 is set to magnetically drive the shearing portion 32 to work, so that a traditional rotating shaft connection structure is omitted, and a sealing structure is not required to be arranged between the culture tank 200 and the power portion 34, so that not only is the sealing performance of the bottom wall 11 improved, but also greasy dirt or germs of the power portion 34 can be completely prevented from entering the tank body of the culture tank 200, the nutrient solution in the culture tank 200 can be ensured to keep a sterile environment for a long time, a stable and reliable environment is provided for the growth of plant tissues, and further the yield of the plant tissues and the quality of the plant tissues in the same culture period are greatly improved.

The power part 34 is provided with a magnetic attraction member rotatable around its central axis. The center axis of the power portion 34 is disposed coincident with the center axis of the second blade 324. The magnetic attraction member restrains the second blade 324 with a magnetic field across the bottom wall 11 and rotates together.

Alternatively, the power section 34 is provided with a stator and a rotor that is drivable by the stator. The stator is fixed to the outer side surface of the bottom wall 11. The rotor and the stator are mutually sleeved and matched, and a gap is reserved between the rotor and the stator. The rotor is energized by an energy source to excite the stator to apply a torque to the rotor relative to the central axis, thereby driving the rotor to variably rotate about the stator.

It is understood that the energy source includes an electrical power source, a high pressure gas or a high pressure liquid, and the like.

In the power section 34, the rotor is provided in a ring shape. And the stator is provided as a cylinder and the diameter of the stator is smaller than the diameter of the rotor annular hole. The rotor is sleeved on the periphery of the stator.

The magnetic attraction member is fixed to an end face of the rotor facing the bottom wall 11. The second blade 324 has an iron piece on its outer periphery that is easily attracted. The magnetic attraction member rotates around the normal of the second blade 324 against the bottom wall 11 and attracts the second blade 324 to rotate together.

Alternatively, the power section 34 may also include a stator and a rotor. The stator is provided in a ring shape and is made of a ferromagnetic material. The middle part of the stator is provided with a mounting hole which penetrates through two ends along the central axis of the stator.

The rotor is provided in a long rod shape. The rotor is sleeved in the mounting hole of the stator, and the rotor can rotate freely in the mounting hole of the stator. The magnetic attraction member is fixed in the rotor at an end facing the bottom wall 11.

Preferably, one end of the rotor is wound with a coil, and the other end is connected with the magnetic attraction piece. The magnetic attraction piece is cylindrical. The second blade 324 is further provided with a cylindrical transmission part 33, one end of the transmission part 33 is connected with the second blade 324, and the other end of the transmission part is sleeved on the periphery of the magnetic attraction piece.

Alternatively, the power section 34 includes a stator and a rotor. The rotor is wound with a plurality of coils. Energizing the coil creates a magnetic pole on the rotor that rotates about its central axis.

Specifically, the plurality of coils are uniformly distributed centering on the rotor, and only one of the coils is energized. Then, the energization is switched between the coils in a clockwise or counterclockwise order, so that a magnetic pole whose direction is continuously changed around its central axis can be generated on the rotor.

The stator is made of a magnetic material and generates a magnetic field with stable magnetic poles. The rotor is electrified to generate rotating magnetic poles, and then the rotor is driven to rotate relative to the stator by attraction force and repulsion force between the magnetic poles.

In this embodiment, a coil is provided on the rotor, and a magnetic pole can be generated by energizing the rotor. The coil energized is switched synchronously by the rotation of the rotor by the same pole repulsion and different pole attraction, so that the magnetic poles on the rotor are always inconsistent with the magnetic poles of the rotor, and the torque applied to the rotor by the stator is continuously acted, thereby greatly improving the function conversion efficiency of the power part 34.

Alternatively, the power section 34 includes a stator and a rotor, and the high pressure medium flows between the stator and the rotor and applies work to the rotor, causing the rotor to rotate relative to the stator.

Specifically, the stator is provided with a passage for conveying a high-pressure medium and a plurality of chambers. The passage communicates with all of the chambers and can convey high pressure medium between the chambers. At least a portion of the rotor is positioned between the chambers and conceals the passageways. The high pressure medium flows from the passage to the adjacent chamber and may exert a force on the rotor. The rotor is pushed by the high-pressure medium to rotate relative to the stator.

The high pressure medium pushes the stator from one chamber into the other and loses a portion of the pressure. The pressure is converted into kinetic energy of the rotor.

Preferably, the high pressure medium comprises a high pressure gas or a high pressure liquid.

The transmission part 33 is arranged between the shearing part 32 and the power part 34, one end of the transmission part 33 is connected with the shearing part 32, and the other end is arranged close to the power part 34. The power part 34 transmits torque to the transmission part 33 through magnetic force and can drive the transmission part 33 to rotate around the pin 321.

The second blade 324 is connected to the inner circumferential surface of the transmission part 33 at a longitudinal end.

Specifically, the transmission portion 33 has a cylindrical shape, and a center portion thereof has a hole penetrating both ends along a center axis thereof. The second blade 324 is inserted into the hole of the transmission part 33. The second blade 324 is connected with both ends thereof to the inner surface of the hole, and the length direction of the second blade 324 is parallel to the diameter of the hole.

In this embodiment, the cutting portion 32 is embedded in the hole of the transmission portion 33, and the transmission portion 33 is connected with the second blade 324 and isolated from the first blade 323, so that the second blade 324 can be driven to rotate relative to the first blade 323, and the plant tissue can be pushed to circulate along the axial direction of the transmission portion 33, thereby avoiding the plant tissue from being repeatedly cut, being beneficial to ensuring the length of the plant tissue and improving the quality of the plant tissue.

The shearing part 32 and the spacer 31 are arranged at intervals along the axial direction of the pin 321. The end face of the shearing part 32 and the end face of the spacer 31 together with the inner peripheral surface of the transmission part 33 enclose a chamber for accommodating materials.

The transmission part 33 is further provided with a discharge opening 333, and the discharge opening 333 is provided on a side wall of the transmission part 33 and penetrates the transmission part 33 in a radial direction. The transmission part 33 may be provided with a plurality of discharge ports 333. The discharge openings 333 are uniformly distributed on the side wall of the transmission part 33 in the circumferential direction.

In particular, in the axial direction of the transmission portion 33, the discharge opening 333 is located between the shearing portion 32 and the spacer 31. I.e. the length of the discharge opening 333 in the axial direction of the transmission part 33 is smaller than the distance between the shearing part 32 and the spacer 31.

The transmission part 33 is formed by abutting the discharge section 331 and the driving section 332.

Specifically, the discharge section 331 is sleeved on the pin 321. And the discharge section 331 is provided with said discharge opening 333 on the side wall. The driving section 332 is sleeved outside the spacer 31. And one end of the driving section 332 is flush with the top surface of the spacer 31.

Further, in order to avoid the plant tissue from being blocked by the side wall of the transmission part 33, the plant tissue is quickened to flow out from the discharge hole 333 after being cut by the cutting part 32. A plurality of discharge openings 333 are provided uniformly distributed on the side wall of the discharge section 331 in the circumferential direction. In particular, the end of the second blade 324 is connected to the side wall between adjacent discharge openings 333 so that the cut-out on the cutout 32 is aligned with the discharge openings 333.

The guide cover 35 is made of a thin-walled material and is sleeved on the periphery of the shearing part 32.

The air guide sleeve 35 is also provided with an inlet 351 and a mounting opening 352 which are mutually parallel. And the inlet 351 and the mounting 352 are parallel to the circular surface formed when the blade rotates.

In this embodiment, by providing the air guide cover 35 on the outer periphery of the shearing portion 32, it is possible to promote the plant tissue to flow from one side of the shearing portion 32 to the other side, increase the circulation flow rate of the plant tissue, and at the same time, it is possible to increase and promote the circulation flow area of the nutrient solution in the culture tank 200, ensure that the cut plant tissue flows from the shearing portion 32 to other areas rapidly, avoid the plant tissue circulating in a small range around the shearing portion 32, thereby reducing the repeated cutting rate of the plant tissue, preventing the occurrence of the problem of uneven length of the plant tissue, and effectively improving the quality of the plant tissue in the culture tank 200.

The spacer 31 penetrates into the pod 35 from the mounting port 352 of the pod 35 and is connected to the shear portion 32. Accordingly, the inlet 351 of the pod 35 is located on the side of the shear 32 facing away from the cup 31 in the axial direction of the shear 32.

In this embodiment, the air guide sleeve 35 is sleeved outside the spacer 31, and the air guide sleeve 35 can be simply, conveniently and rapidly arranged on the culture tank 200 by dismounting the spacer 31, so that the air guide sleeve 35 is convenient to maintain and replace.

In addition, spacer 31 includes cylindrical boss 311. The air guide sleeve 35 is provided with a cylindrical hollow sleeve body and is sleeved on the outer peripheral surface of the convex column 311. A space is provided between the cover surface of the air guide cover 35 and the outer surface of the convex column 311. The space forms an annular channel.

The spacer 31 is further provided with a flange 312 extending outwardly from the end edge of the boss 311. The flange 312 is annular. The pod 35 is coupled to the flange 312.

Preferably, the pod 35 is coupled to the outer annular edge of the flange 312.

The air guide sleeve 35 is provided as a cylindrical thin-walled cylinder. The pod 35 is also provided with cavities penetrating both ends in the direction of the central axis thereof. The inlet 351 and the mounting opening 352 are communicated with the cavity and are respectively positioned at two ends of the thin-walled cylinder.

Further, an outlet 353 is provided on the sidewall of the air guide sleeve 35. When the pod 35 is sleeved on the cutout 32, one end of the outlet 353 is aligned with the cutout 32 and the other end is adjacent to the mounting port 352 in the axial direction.

The inlet 351 directs the plant tissue into the incision of the shearing apparatus 3 and the outlet 353 is used to discharge the sheared plant tissue into the canister.

In this embodiment, the dome 35 is disposed on the outer periphery of the shear part 32, so that the circulating flow area of the nutrient solution in the culture tank 200 is increased, the plant tissue is promoted to circulate in the tank cavity 90 in a large range, the plant tissue in the area near the shear part 32 is prevented from being influenced by the shear part 32 to circulate in a small range, and the culture tank 200 is ensured to be in a good circulating state, so that the plant tissue can be trimmed.

It should be noted that, when the plant tissue enters the shearing device 3 under the action of the air flow, the blades in the shearing device 3 rotate to generate vortex suction force, the plant tissue is sucked into the notch of the shearing device 3 from the inlet 351 of the air guide sleeve 35, and the regularly arranged plant tissue is sheared by the blades, so that the plant tissue is sheared into small segments meeting the requirements, and then is discharged from the outlet 353 of the air guide sleeve 35, so that the plant tissue is prevented from agglomerating and winding together, the shearing effect is affected, and smooth discharging is ensured.

In another embodiment, as shown in fig. 10 to 12, the bioreactor includes a support frame having a certain extension length in all of the length direction, the width direction and the height direction.

The bioreactors are arranged at intervals in the length direction of the supporting frame and are connected in series to form continuous cultivation of plant tissues; the bioreactor may be supported by support legs or by a support frame extending in the width direction.

The support frame is provided with functional pipelines, and comprises a main pipeline 1 and branch pipelines 2, wherein the main pipeline 1 is provided with a plurality of pipelines, a certain extension length is arranged in the length direction of the support frame, the pipelines are arranged side by side in the height direction of the support frame, and each main pipeline 1 is used for supplying different substances; the branch pipe 2 is connected to the main pipe 1, extends in the width direction of the support frame, and is connected to the seed tank 100 and the culture tank 200.

The main pipeline 1 is large in interception and is used for centralized material supply, the branch pipelines 2 are small in interception and are used for conveying the material of the main pipeline 1 to the bioreactor, different main pipelines 1 are arranged in parallel, so that a plurality of branch pipelines 2 can be connected in the extending direction of each main pipeline 1, and the pipelines can be quickly identified during maintenance, thereby improving the regularity of the pipeline structure and reducing the ineffective occupation of the pipelines to the space.

The main pipeline 1 comprises a hot water supply main pipe E200 and a hot water recovery main pipe E100, and the branch pipeline 2 comprises a hot water supply branch pipe E20 and a hot water recovery branch pipe E10 correspondingly; the hot water supply branch pipe E20 has one end communicated with the bioreactor and the other end communicated with the hot water supply main pipe E200 for inputting hot water into the bioreactor. One end of the hot water recovery branch pipe E10 is communicated with the bioreactor, and the other end is communicated with the hot water recovery main pipe E100 for leading out the hot water in the bioreactor.

The main pipeline 1 comprises a chilled water supply main pipe C200 and a chilled water recovery main pipe C100, and the branch pipeline 2 comprises a chilled water supply branch pipe C20 and a chilled water recovery branch pipe C10; the chilled water supply branch pipe C20 is communicated with the bioreactor at one end and the chilled water supply main pipe C200 at the other end for inputting chilled water into the bioreactor, and the chilled water recovery branch pipe C10 is communicated with the bioreactor at one end and the chilled water recovery main pipe C100 at the other end for guiding out chilled water in the bioreactor.

The main pipeline 1 comprises a cooling water supply main pipe D200 and a cooling water recovery main pipe D100, and the branch pipeline 2 comprises a cooling water supply branch pipe D20 and a cooling water recovery branch pipe D10 correspondingly; one end of the cooling water supply branch pipe D20 is communicated with the bioreactor, the other end of the cooling water supply branch pipe D is communicated with the cooling water supply main pipe D200 for inputting cooling water into the bioreactor, one end of the cooling water recovery branch pipe D10 is communicated with the bioreactor, and the other end of the cooling water recovery branch pipe D is communicated with the cooling water recovery main pipe D100 for guiding out the cooling water in the bioreactor.

In this embodiment, the main pipeline 1 and the corresponding branch pipeline 2 are connected to form a complete circulation loop, so that the pipeline structure can exchange heat with the bioreactor, thereby providing stable growth conditions for proliferation of plant tissues, and the pipeline structure is regular due to parallel arrangement of different main pipelines 1, so that the maintenance is convenient.

In order to independently control the material circulation of each bioreactor, the hot water supply branch pipe E20, the chilled water supply branch pipe C20 and the cooling water supply branch pipe D20 are provided with a pneumatic valve 4 for controlling the on-off of a pipeline and a manual valve 5 connected with the pneumatic valve 4 in series, so that the pipeline connected with the bioreactors can realize the automatic control of the on-off state, and meanwhile, the pipeline of the bioreactors can still utilize the manual valve 5 to change the on-off state when the pneumatic valve 4 fails or is maintained, so that the continuous work of the bioreactors is maintained, and the reliability of the pipeline structure is improved.

Similarly, the hot water recovery branch pipe E10, the chilled water recovery branch pipe C10 and the cooling water recovery branch pipe D10 are provided with a pneumatic valve 4 and/or a manual valve 5 for controlling on-off of a pipeline.

The pneumatic valves 4 and/or the manual valves 5 on the hot water recovery branch pipe E10, the chilled water recovery branch pipe C10 and the cooling water recovery branch pipe D10 are correspondingly matched with the pneumatic valves 4 and/or the manual valves 5 on the hot water supply branch pipe E20, the chilled water supply branch pipe C20 and the cooling water supply branch pipe D20, so that a complete circulation loop is formed.

The hot water supply branch pipe E20, the chilled water supply branch pipe C20 and the cooling water supply branch pipe D20 are connected with an input pipe 6 arranged on the bioreactor, and various branch pipes are connected with the bioreactor through the input pipe 6, so that the connecting ports on the bioreactor are reduced, the difficulty in manufacturing the bioreactor can be reduced, and the tightness of the bioreactor can be improved.

In order to improve the safety and reliability of the pipeline structure, a loop capable of directly discharging high-heat medium is additionally arranged on the pipeline structure, so that the maintenance of the circulation loop is facilitated. The main pipe 1 is further provided with a heat drain pipe F2 provided with a pressure reducing filter 410. A discharge branch 420 communicating with the depressurization filter 410 is provided between the input pipe 6 and the branch pipe 2, and the medium supplied in the main pipe 1 may be directly introduced into the heat drain pipe F2 without passing through the bioreactor.

In another embodiment, a temperature regulating system of a bioreactor is described, as shown in fig. 10 to 12, the temperature regulating system comprises a supply unit A1, a temperature control layer 9 coated on the outer surface of the bioreactor, and a conveying unit A2 communicating the supply unit A1 and the temperature control layer 9. The supply unit A1 carries a plurality of liquids having different temperatures, and introduces and removes the temperature control layer 9 via the transport unit A2. The supply unit A1 comprises a plurality of main pipelines 1 arranged in parallel, and each main pipeline 1 is used for respectively leading in and leading out different substances to the bioreactor or the temperature control layer 9.

The tempering system further comprises a tempering tank A3 in communication with the supply unit A1. The temperature-adjusting water tank A3 is connected to the supply unit A1 in parallel with the bioreactor. The supply unit A1 is capable of carrying a plurality of liquids having different temperatures and introducing and discharging the liquid into and from the temperature-adjusting water tank A3 via the conveying unit A2. The water in the temperature-regulating water tank A3 circulates to the temperature-controlling layer 9 to exchange heat with the bioreactor and then flows back to the temperature-regulating water tank A3.

The temperature regulating system is additionally provided with the temperature regulating water tank A3, and the temperature regulating water tank A3 and the temperature control layer 9 are connected to the supply unit A1, so that the temperature regulating water tank A3 and the temperature control layer 9 form a loop connected with the supply unit A1 in parallel, the temperature regulating water tank A3 and the temperature control layer 9 can exchange heat medium with the supply unit A1 at the same time, and the temperature regulating water tank A3 and the temperature control layer 9 can be connected with the supply unit A1 in series to form a temperature regulating loop, so that the temperature regulating water tank A3 directly circulates with the temperature control layer 9 and is equivalent to a standby heat supply source, the temperature regulating loop can be used for independently regulating the temperature of the bioreactor when the supply source of the temperature regulating system is damaged and maintained, and the fault tolerance of the temperature regulating system is improved.

Specifically, the temperature-adjusting water tank A3 is provided with a water containing cavity a301 for containing and heating the liquid which can be input into the temperature-controlling layer 9, so that a circulation loop which can supply heat to the temperature-controlling layer 9 is formed between the temperature-adjusting water tank A3 and the temperature-controlling layer 9.

Wherein, the water containing cavity A301 and the temperature control layer 9 are connected with the supply unit A1 through the conveying unit A2 to form a circulation loop. The conveying unit A2 comprises a water inlet pipe A21 and a water outlet pipe A22 which are communicated with the supply unit A1 and the water containing cavity A301, and the temperature of the culture solution in the bioreactor can be maintained by the temperature regulating water tank A3.

The temperature-regulating water tank A3 is provided with a heat exchange structure A23 which is arranged close to the water containing cavity A301. The conveying unit A2 comprises a liquid inlet pipe A24 and a liquid outlet pipe A25 which are communicated with the supply unit A1 and the heat exchange structure A23 and are used for respectively leading in and leading out liquids with different temperatures to the heat exchange structure A23. Through setting up heat transfer structure A23 in the temperature-regulating water tank A3 for the hot water in the temperature-regulating water tank A3 keeps the settlement temperature always when supply unit A1 operates, thereby ensures that the temperature-regulating water tank A3 can be immediately to the heat control layer 9 when supply unit A1 stops supplying or maintaining, need not wait for the water of temperature-regulating water tank A3 to heat, guarantees that the reserve heat transfer circuit of temperature-regulating system can take over main heat transfer circuit to maintain the normal temperature of the culture solution in the bioreactor fast.

Of these, the supply unit A1 is a main line 1, and the delivery unit A2 is a branch line 2.

The water inlet pipe A21 is connected with the hot water supply main pipe E200, the water outlet pipe A22 is connected with the hot water recovery main pipe E100, and the water containing cavity A301 and the temperature control layer 9 are connected in series in the same circuit.

The water inlet pipe A21 is internally and serially provided with a water pump A211 for conveying water in the water containing cavity A301 to the temperature control layer 9, and the water pump A211 can independently circulate hot water by providing power for a circulation loop formed by the temperature control water tank A3 and the temperature control layer 9.

In order to reduce the resistance of the hot water being transported between the supply unit A1 and the tempering tank A3 and to avoid that the water pump a211 blocks the hot water from flowing into the tempering tank A3, a branch pipe a212 connected in parallel with the water pump a211 is provided on the water inlet pipe a 21. Both ends of the branch pipe A212 are respectively connected with a water inlet end and a water outlet end of the water pump A211 communicated with the water inlet pipe A21, so that hot water flowing into the temperature-adjusting water tank A3 from the supply unit A1 bypasses the water pump A211 from the branch pipe A212, and the resistance of the supply unit A1 for conveying the hot water to the temperature-adjusting water tank A3 is reduced.

In order to reduce the temperature of the hot water in the tempering tank A3, the supply unit A1 comprises a chilled water supply main C200 and a cold drain pipe F1 arranged in parallel for delivering to the heat exchange structure a23 and for heat transfer with the hot water in the water containing chamber a 301.

The liquid inlet pipe A24 comprises a chilled water liquid inlet pipe A241, the liquid outlet pipe A25 comprises a chilled water liquid outlet pipe A251, one end of the chilled water liquid inlet pipe A241 is connected with the chilled water supply main pipe C200, the other end of the chilled water liquid inlet pipe A241 is connected with the heat exchange structure A23, one end of the chilled water liquid outlet pipe A251 is connected with the heat exchange structure A23, and the other end of the chilled water liquid outlet pipe A251 is connected with the cold drain pipeline F1.

In order to raise the temperature of the hot water in the tempering tank A3, the supply unit A1 comprises a main steam supply pipe B100 and a heat drain pipe F2 arranged in parallel for delivering to the heat exchange structure a23 and for heat transfer with the hot water in the water containing chamber a 301.

The liquid inlet pipe A24 comprises a steam liquid inlet pipe A242, the liquid outlet pipe A25 comprises a steam liquid outlet pipe A252, one end of the steam liquid inlet pipe A242 is connected with the steam supply main pipe B100, the other end of the steam liquid inlet pipe A242 is connected with the heat exchange structure A23, one end of the steam liquid outlet pipe A252 is connected with the heat exchange structure A23, and the other end of the steam liquid outlet pipe A252 is connected with the heat drain pipe F2.

Two parallel branches are arranged on the steam liquid inlet pipe A242: one is connected with a manual valve 5 and a pneumatic valve 4 in series, and the other is connected with the manual valve 5 only in series, so as to manually control the on-off of the steam liquid inlet pipe A242 in the event of automatic control failure.

The steam liquid outlet pipe A252 is split into two pipelines at one end connected with the temperature-regulating water tank A3, wherein one pipeline is connected with a one-way valve and a pneumatic valve 4 in series, and the other pipeline is connected with the pneumatic valve 4 only in series.

Through set up two parallelly connected pipelines that are used for controlling the break-make state on steam feed liquor pipe A242 and steam drain pipe A252 respectively for the steam circulation loop of tempering tank A3 not only can realize automated control through pneumatics, can be through the break-make of manual switching steam circulation loop when automatic control became invalid or maintained again, avoided unable use the condition of supply unit A1 heating in the tempering tank A3, improved temperature regulating system's stability, reliability.

In another embodiment, a gas supply system of a bioreactor is described, and as shown in fig. 6, 10 to 12, the gas supply system comprises a gas supply main pipe a100 and a gas inlet branch pipe a110 connected with the gas supply main pipe a100, and is used for conveying gas for culture; also included is a main steam supply pipe B100 and a filtering branch a140 connected to the inlet branch a110 for delivering high temperature steam for sterilization.

The bottom wall 11 of the bioreactor is obliquely arranged, and the height of the bottom wall 11 gradually decreases from the periphery to the center to form a conical body with a large upper part and a small lower part.

The bottom wall 11 is provided with a gas distributor 40, and the gas distributor 40 is alternatively connected to the steam supply main pipe B100 and the gas supply main pipe a100, and high-temperature steam for sterilization or gas for culture is respectively introduced into the bioreactor.

The gas inlet pipeline of the gas distributor 40 is respectively connected with the steam supply main pipe B100 and the gas supply main pipe A100 through three-way valves.

Alternatively, the inlet line of the gas distributor 40 is connected to the inlet branch a 110. A check valve a120 may be further disposed between the gas distributor 40 and the gas inlet branch a110, so that the gas inlet branch a110 communicated with the bioreactor can only unidirectionally convey gas to the bioreactor, thereby avoiding backflow of plant tissues and culture fluid in the bioreactor into the pipeline.

Since the gas contains a large amount of impurities, microorganisms and germs, in order to avoid that microorganisms and germs and the like multiply in the culture solution and consume nutrients in the culture solution, even plant tissue is diseased and spoiled, a filtering device A130 is arranged in the gas supply system.

Specifically, the filter device a130 is disposed in series in the intake branch a 110. Thus, germs and impurities are filtered out when the air passes through the filtering device A130, and the air entering the bioreactor is largely purified.

The filter element of the existing filter device A130 basically adopts polytetrafluoroethylene and other materials, and the filter element made of polytetrafluoroethylene is easy to break down by impurities and the like after being used for a period of time, so that the filter element loses the capability of filtering germs and impurities, and the service life of the filter element is short. In order to extend the service life of the filter cartridge and increase the period of maintenance of the filter device a130, the filter cartridge is provided to be made of silicate material.

In the embodiment, the filter element is made of silicate materials by improving the filter element materials, the strength of the filter element is improved by utilizing the characteristics of high hardness and high strength of silicate, the service life of the filter element is greatly prolonged, the period of maintaining the filter device A130 is prolonged, and the bioreactor can work for a longer time.

The connection end of the filtering branch a140 and the air inlet branch a110 is arranged in front of the connection end of the inlet of the filtering device a130 and the air inlet branch a 110.

In this embodiment, the filtering branch circuit a140 is used to connect the filtering device a130 and the steam supply main pipe B100, so that the filtering device a130 can be sterilized by introducing high-temperature steam before the air supply system is started, thereby ensuring that germs, impurities and the like on the filtering device a130 are cleaned up and are in good working conditions, greatly improving the filtering effect of the filtering device a130, ensuring the cleanliness of air entering the bioreactor, and being beneficial to maintaining good growth environment of the bioreactor.

The steam supply main pipe B100 further comprises a tank elimination branch A150, wherein one end of the tank elimination branch A150 is connected to the steam supply main pipe B100, and the other end of the tank elimination branch A150 is connected to the rear of the connection end of the outlet of the filtering device A130 and the air inlet branch A110. Therefore, the steam supply main pipe B100 and the air supply main pipe A100 share a pipeline connected with the bioreactor, the connecting ports arranged on the bioreactor are reduced, the difficulty in manufacturing the bioreactor can be reduced, and the tightness of the bioreactor can be improved.

A standby branch a160 for manually switching on/off state is also provided, i.e. a standby control loop is added. The inlet end of the standby branch A160 is connected with the main air supply pipe A100, and the outlet end of the standby branch A160 is connected before the connection end of the filtering branch A140 and the air inlet branch A110. So that the backup branch a160 is connected to the main air supply pipe a100 in parallel with the intake branch a110 and is simultaneously communicated to the front of the filter device a 130.

To keep the air pressure in the bioreactor stable, the air supply system further comprises an air discharge line a170. One end of the exhaust pipeline A170 is connected with the top of the bioreactor, and the other end of the exhaust pipeline A170 is communicated with the outside, so that the waste gas in the bioreactor can be directly discharged into the atmosphere.

The exhaust pipeline A170 is also provided with a gas filter A180 in series for trapping moisture and nutrients contained in the gas in the bioreactor.

In order to ensure that the gas filter a180 is in a low temperature state for a long time to improve the effect of filtering moisture, nutrients and nutrients in the exhaust gas, the gas filter a180 is provided with a cooling structure for coating the filter element, so as to cool the gas filter a 180.

Preferably, as shown in fig. 13, when four gas distributors 40 are uniformly distributed on the bottom wall 11 of the culture tank 200, two gas inlet branches a110 are provided, one end of each gas inlet branch a110 is connected with the gas supply main pipe a100, the other end is connected with two branches a1107 through a three-way pipe B8, the extending length and the extending direction of each branch a1107 are the same and are respectively connected with one gas distributor 40, and the gas distributors 40 connected with the two branches a1107 of one gas inlet branch a110 are symmetrically arranged on the bottom wall 11. Thus, the uniformity of the air intake of the air distributor 40 into the culture tank 200 is ensured, and the culture quality of the plant tissue is improved.

The arrangement of the filtering and eliminating branch A140 can be also the same, so as to ensure the sterilization effect.

In another embodiment, as shown in fig. 14 to 19, the seed tank 100 and the culture tank 200 are connected by at least one seed transfer line 300 and at least one seed return line 900, respectively.

The seed transfer line 300 is used to transfer the culture solution containing plant tissue in the seed tank 100 to the culture tank 200.

The reseeding line 900 is used to transfer a portion of the culture solution containing plant tissue in the culture tank 200 back into the seed tank 100.

In the present embodiment, by providing at least one transplanting line 300 between the seed tank 100 and the culture tank 200, the plant tissue cultivated to a certain extent in the seed tank 100 is transferred into the culture tank 200 by using the transplanting line 300, and the continuous cultivation is achieved; through setting up at least one returns kind of pipeline 900 between seed tank 100 and culture tank 200, utilize returning kind of pipeline 900 to turn back the partial culture solution that contains plant tissue in the culture tank 200 to in the seed tank 100, so, provide new seed again to the seed tank 100 in, whole process need not with the help of other equipment, and do not contact external environment, not only reduced the probability of dying the fungus, improved seed cultivation's success rate, simultaneously, reduced the use of equipment, the cost is reduced, and convenient operation.

The concentration of the culture solution containing plant tissues in the seed transfer line 300 is greater than the concentration of the culture solution containing plant tissues in the seed returning line 900 during seed returning.

In this embodiment, when plant tissue in the seed tank 100 is cultured to a certain extent, the culture solution containing plant tissue in the seed tank 100 is transferred into the culture tank 200 by using the transplanting line 300, and then part of the culture solution containing plant tissue in the culture tank 200 is transferred back into the seed tank 100 by using the reseeding line 900, and since the volume of the culture tank 200 is much larger than that of the seed tank 100, the concentration of the culture solution containing plant tissue in the transplanting line 300 is larger than that of the reseeding line 900; when the low-concentration plant tissue-containing culture solution is cultured to a certain extent in the seed tank 100, the seed transfer and seed returning operations are performed again, thereby realizing the cyclic culture.

The seed tank 100 and the culture tank 200 respectively comprise tank bodies, the bottom wall 11 of each tank body is obliquely arranged and is in a conical shape, and a discharge hole is formed in the lowest position of the center of the bottom wall 11.

The ratio of the height to the diameter of the tank body is 1:1, wherein the height is from the top of the tank body to the lowest part of the bottom wall 11, and is represented by H; the diameter is the diameter of the inner peripheral wall of the can body, denoted by D.

The height and diameter of the tank body are set so that the pressure at the bottom is not too high when plant tissues are cultured in the tank body, the plant tissues are prevented from being mutually extruded, and the growth of the tissues is facilitated.

One end of the seed returning pipeline 900 is connected to a discharge port at the bottom of the culture tank 200, and the other end is connected to the top or upper side wall of the seed tank 100, preferably to the upper side wall.

The highest point of the seed returning line 900 is located at a level lower than the level at the top of the seed tank 100.

In this embodiment, the other end of the seed returning pipeline 900 is connected to the side wall of the seed tank 100, so that the level at the highest point of the seed returning pipeline 900 is lower than the level at the top of the seed tank 100, which is convenient for reducing the requirement on the pressure difference between the seed tank 100 and the culture tank 200 in the subsequent seed returning process, and simultaneously reducing the seed returning height, preventing the culture solution containing plant tissues from splashing on the tank wall during the transferring process to cause waste, and reducing the difficulty in cleaning the seed tank 100.

The side wall of the seed tank 100 is provided with a connecting port 120, the other end of the seed returning pipeline 900 is provided with a material returning part 920 communicated with the seed returning pipeline 900, and the material returning part 920 extends into the seed tank 100 through the connecting port 120, and the extending direction and the horizontal direction form an included angle.

The material returning part 920 is in a tubular structure, has an extension length, and is inclined to the gravity center direction of the seed tank 100, i.e. is arranged in a downward extending manner, and the material outlet of the material returning part 920 is higher than the gravity center of the seed tank 100 and is always higher than the level of the culture solution in the seed tank 100.

In this embodiment, by providing the return portion 920 at the other end of the seed returning line 900, the return portion 920 is extended downward, so that not only the seed returning height is further reduced, but also the culture solution containing plant tissue is directly sprayed downward into the seed tank 100 without being sprayed toward the tank wall, and the splashing and waste are reduced; the discharge hole of the feed back portion 920 is always higher than the level of the culture solution in the seed tank 100, so as to prevent the liquid level of the culture solution in the seed tank 100 from passing through the discharge hole during the seed returning process, thereby preventing the occurrence of the backflow phenomenon and ensuring the smooth seed returning process.

The material returning portion 920 may be a section of straight pipe, or a section of pipeline with radian, etc., and may be set as required.

The seed returning pipeline 900 is connected with the connection port 120 in a sealing manner, so as to prevent liquid leakage and air leakage.

The bottom of the culture tank 200 is provided with a tank bottom valve 110, the tank bottom valve 110 is vertically arranged and at least partially positioned in the seed returning pipeline 900, and the top of the tank bottom valve 110 is positioned at the joint of the discharge port and the seed returning pipeline 900 and is used for opening or closing the discharge port.

The seed returning pipeline 900 is provided with a seed returning valve 910 and a drain valve for discharging the cleaning waste liquid, and the seed returning valve 910 is matched with the tank bottom valve 110 and the drain valve for opening or closing the seed returning pipeline 900.

The seed returning pipeline 900 is connected with a functional pipeline, the connection part of the functional pipeline and the seed returning pipeline 900 is close to the position where the seed returning pipeline 900 stretches into the side wall of the seed returning pipeline 900, and the connection part is located upstream of the seed returning valve 910 along the seed moving direction.

The functional line can alternatively be connected to one of a steam line 500 for supplying high temperature steam, a CIP cleaning line 400 for supplying acid/alkali cleaning solution, and a water cleaning line 1000 for supplying cleaning water.

The functional pipeline is selectively communicated with the steam pipeline 500, the CIP cleaning pipeline 400 and the water washing pipeline 1000 through four-way valves.

Valves are provided on the CIP cleaning line 400, the water wash line 1000, and the steam line 500, respectively.

The device further comprises a five-way valve 320 arranged on the seed returning pipeline 900, wherein the five-way valve 320 is provided with five interfaces, two interfaces are respectively connected with the seed returning pipeline 900, and the remaining three interfaces are respectively connected with the discharging pipeline 600, the sewage draining pipeline 700 and the CIP discharging pipeline 800.

The drain valves on the return line 900 for draining the cleaning waste, i.e., the drain line 700 and the CIP drain line 800.

It should be noted that, in the process of reseeding, the culture solution containing plant tissue flows through the inside of the five-way valve 320, so that before and after reseeding, the five-way valve 320 needs to be cleaned and sterilized, that is, the functional pipeline connected to the reseeding pipeline 900 is utilized, and the reseeding pipeline 900, the five-way valve 320, the reseeding valve 910 and the tank bottom valve 110 are cleaned and sterilized.

The discharging pipeline 600 is used for discharging, a discharging valve 610 is arranged on the discharging pipeline, the discharging valve 610 is matched with the tank bottom valve 110, and the discharging pipeline 600 is opened or closed; when the plant tissue in the culture tank 200 is cultured to a certain extent and meets the discharging requirement, the tank bottom valve 110 and the discharging valve 610 are opened, namely the discharging pipeline 600 is opened, and the plant tissue is conveyed to the next processing equipment to finish discharging.

The drain pipe 700 is used for draining, a drain valve 710 is arranged on the drain pipe, the drain valve 710 is matched with the tank bottom valve 110, and the drain pipe 700 is opened or closed; when it is desired to drain contaminants in the tank, such as clean water, steam condensate, etc., the tank bottom valve 110 and the drain valve 710 are opened.

The CIP discharge pipe 800 is used for discharging acid/alkali washing liquid, a CIP discharge valve 810 is arranged on the CIP discharge pipe, the CIP discharge valve 810 is matched with the tank bottom valve 110, and the CIP discharge pipe 800 is opened or closed; after the pickling or alkaline washing of the inside of the tank is completed, the tank bottom valve 110 and the CIP drain valve 810 are opened to drain the pickling or alkaline washing liquid, which requires a separate pipe to drain and treat since the pickling or alkaline washing liquid has a certain acidity and alkalinity, thereby preventing environmental pollution.

It should be noted that only the tank and the pipeline in the new state are subjected to acid/alkali washing, passivation, cleaning and sterilization; in the subsequent culture process, the culture medium is only subjected to water washing and steam sterilization.

In this embodiment, when acid/alkali washing is performed on the reseeding pipeline 900, the tank bottom valve 110 and the reseeding valve 910 are closed, the five-way valve 320 and the CIP discharge pipeline 800 are opened, then the four-way valve and the valves on the CIP cleaning pipeline 400 are opened, acid/alkali washing liquid is introduced into the reseeding pipeline 900, the acid/alkali washing liquid flows in the pipeline for cleaning, and the acid/alkali washing liquid is discharged from the CIP discharge pipeline 800;

when the reseeding pipeline 900 is washed by water, the tank bottom valve 110 and the reseeding valve 910 are closed, the five-way valve 320 and the drain valve 710 are opened, then the four-way valve and the valves on the washing pipeline 1000 are opened, washing water is introduced into the reseeding pipeline 900, flows in the pipeline for washing, and is discharged from the drain pipeline 700;

when the seed returning pipeline 900 is subjected to steam sterilization, the tank bottom valve 110 and the seed returning valve 910 are closed, the five-way valve 320 and the sewage valve 710 are opened, then the four-way valve and the valves on the steam pipeline 500 are opened, high-temperature steam is introduced into the seed returning pipeline 900, the steam diffuses and flows in the pipeline, condenses after contacting with the pipe wall to achieve a sterilization effect, and is discharged from the sewage pipeline 700;

After the sterilization is completed, the tank bottom valve 110 and the seed returning valve 910 are opened, and the other valves are closed, so that the culture solution containing plant tissues flows out from the discharge port under the action of pressure difference and flows into the seed tank 100 through the seed returning pipeline 900.

When the steam is utilized for sterilization, the five-way valve 320 and the blow-off valve 710 are opened to have the function of releasing pressure on the seed returning pipeline 900, so that the steam can be ensured to enter continuously smoothly, the whole seed returning pipeline 900 is filled, and the sterilization and disinfection effects are ensured.

The device also comprises a liquid level detector, a pressure detector and a control module.

The liquid level detector is arranged on the seed tank 100 and/or the culture tank 200, and is used for detecting the liquid level of the culture solution in the tank.

The pressure detector is provided on the seed tank 100 and the culture tank 200 for detecting the pressure in the tank.

The control module is used for opening the tank bottom valve 110 and the seed returning valve 910 when the pressure difference between the seed tank 100 and the culture tank 200 reaches the preset seed returning pressure difference; when the level of the culture medium in the seed tank 100 and/or the culture tank 200 reaches a preset reseeding level, the tank bottom valve 110 and the reseeding valve 910 are closed.

Further, the bottom valve 110 is also disposed at the outlet of the seed tank 100 in the same manner as the bottom of the culture tank 200.

One end of the seed transfer pipeline 300 is connected with the discharge port of the seed tank 100, the other end is connected with the side wall of the culture tank 200, and the horizontal plane of the highest point of the seed transfer pipeline 300 is lower than the horizontal plane of the top of the culture tank 200.

The other end of the seed transfer pipeline 300 is provided with a discharging part 330, the discharging part 330 is in a tubular structure, one end of the discharging part 330 is communicated with the seed transfer pipeline 300, the other end of the discharging part is inclined towards the gravity center direction of the culture tank 200, namely, the discharging part extends downwards, and a discharging port of the discharging part 330 is higher than the gravity center of the culture tank 200 and is always higher than the level of the culture solution in the culture tank 200.

In this embodiment, one end of the discharging part 330 is communicated with the seed transferring pipeline 300, and the other end is extended downwards, so that not only the seed transferring height is further reduced, but also the culture solution containing plant tissues is directly sprayed downwards into the culture solution in the culture tank 200, and the culture solution is not sprayed towards the tank wall, so that the splashing and waste are reduced; the discharge port of the discharge portion 330 is higher than the gravity center of the culture tank 200 and always higher than the level of the culture solution in the culture tank 200, so as to prevent the liquid level of the culture solution in the culture tank 200 from overflowing one end of the discharge portion 330 during the seed transplanting process, thereby preventing the occurrence of the backflow phenomenon and ensuring the normal seed transplanting process.

Since the sterile environment is required for cultivation, the transfer line 300 is also provided with a cleaning and disinfection process capable of selecting one of the CIP cleaning line 400, the steam line 500, and the water washing line 1000 for communication with each other, and cleaning and disinfecting the transfer line 300.

The steam pipeline 500 on the seed transfer pipeline 300 and the steam pipeline 500 on the seed returning pipeline 900 can be connected to the same main steam pipeline and then connected to the same steam supply device, or can be connected to respective steam supply devices; the same is true of CIP cleaning line 400 and water wash line 1000.

Also, the five-way valve 320 is required to be disposed on the seed transfer pipeline 300, and is used for communicating with the discharging pipeline 600, the sewage draining pipeline 700 and the CIP discharging pipeline 800, so as to realize discharging and sewage draining, which has the same function as the five-way valve 320 on the seed returning pipeline 900.

When the seed transfer pipeline 300 is subjected to acid/alkali washing, the tank bottom valve 110 and the seed transfer valve 310 are closed, the five-way valve 320 and the CIP discharge pipeline 800 are opened, then the four-way valve and the valves on the CIP cleaning pipeline 400 are opened, acid/alkali washing liquid is introduced into the seed transfer pipeline 300, flows in the pipeline for washing, and is discharged from the CIP discharge pipeline 800;

When the seed transfer pipeline 300 is washed with water, the tank bottom valve 110 and the seed transfer valve 310 are closed, the five-way valve 320 and the drain valve 710 are opened, then the four-way valve and the valves on the water washing pipeline 1000 are opened, washing water is introduced into the seed transfer pipeline 300, flows in the pipeline for washing, and is discharged from the drain pipeline 700;

when the seed transfer pipeline 300 is subjected to steam sterilization, the tank bottom valve 110 and the seed transfer valve 310 are closed, the five-way valve 320 and the drain valve 710 are opened, then the four-way valve and the valves on the steam pipeline 500 are opened, high-temperature steam is introduced into the seed transfer pipeline 300, the steam diffuses and moves in the pipeline, condenses after contacting with the pipe wall to achieve a sterilization effect, and is discharged from the drain pipeline 700;

after the sterilization is completed, the bottom valve 110 and the seed transfer valve 310 are opened, and the other valves are closed, so that the culture solution containing plant tissues flows out from the discharge port under the action of the pressure difference and flows into the culture tank 200 through the seed transfer pipeline 300.

The top center position of the tank body is provided with a water inlet 220 for feeding cleaning water into the tank body and cleaning the inside of the tank body.

The top of the tank body is provided with a maintenance port 210 near the side wall, the maintenance port 210 is large in size, and maintenance workers can enter the tank body to maintain the components in the tank body.

The top of the tank body is also provided with an inoculation port 230 near the side wall, and a sterilizing device is arranged at the inoculation port 230. Because of practical needs, seeds in the seed tank 100 can be returned from the culture tank 200, and can also be inoculated from the inoculation port 230, so that various feeding modes are realized, and the use experience of users is improved.

As shown in fig. 12, the top of the tank body is further provided with an air outlet, which is communicated with the air exhaust pipeline a 170.

As shown in fig. 1, 15 and 17, a temperature control layer 9 is arranged on the outer side of the peripheral wall of the tank body, the upper side of the temperature control layer 9 is positioned below the connecting port 120, and the lower side of the temperature control layer 9 extends to the inclined bottom wall 11 and is positioned above the gas distributor 40 and the shearing device 3; the temperature control layer 9 is filled with a cooling medium or a heating medium, and the temperature in the tank body is controlled within a proper temperature range required by culture.

The components provided in the seed tank 100 may be provided in the culture tank 200, and the components provided in the culture tank 200 may be provided in the seed tank 100, and may have the same or different structures except for different volumes, and may be set as needed.

Before or after the seed tank 100 and the culture tank 200 are used, cleaning and sterilizing operations are required, and likewise, before or after the seed transfer and seed returning, cleaning and sterilizing operations are required for the seed transfer pipeline 300 and the seed returning pipeline 900, so that the whole culture system reaches the sterile standard and meets the culture conditions.

And the density detector is arranged on the seed tank 100 and/or the culture tank 200 and is used for detecting the culture density of plant tissues in the tank.

The process of seed transfer and seed returning is briefly described as follows:

the seed transfer process comprises the following steps: firstly, acid/alkali washing and water washing treatment are carried out on a seed tank 100, a culture tank 200 and a seed transferring pipeline 300, then a certain volume of culture solution is added into the seed tank 100, and high-temperature steam is conveyed into the seed tank 100 by a gas distributor 40 for sterilization treatment; inoculating into the seed tank 100, and shearing the plant tissue by the shearing device 3 to reduce the size of the plant tissue when the plant tissue grows to a certain extent; meanwhile, the seed transfer line 300 is sterilized by the steam line 500; adding a certain volume of culture solution into the culture tank 200, and delivering high-temperature steam into the culture tank 200 by using the gas distributor 40 for sterilization; after the culture tank 200 is maintained at a proper temperature, the pressures of the seed tank 100 and the culture tank 200 are changed, when the pressure difference satisfies the seed transfer requirement, the tank bottom valve 110 below the seed tank 100 and the seed transfer valve 310 on the seed transfer line 300 are opened, the plant tissue seeds in the seed tank 100 are transferred to the culture tank 200 for continuous culture, and when the liquid level of the seed tank 100 or the culture tank 200 reaches a preset seed transfer liquid level, the tank bottom valve 110 and the seed transfer valve 310 are closed. After the completion of the seed transfer, the seed tank 100 and the transfer line 300 are required to be cleaned and sterilized again.

The return process comprises the following steps: after the seed moving operation is completed, the pressures of the seed tank 100 and the culture tank 200 are changed, when the pressure detector detects that the pressure difference meets the seed returning requirement, the seed returning pipeline 900 is cleaned and steam sterilized, the tank bottom valve 110 below the culture tank 200 and the seed returning valve 910 on the seed returning pipeline 900 are opened, part of culture solution containing plant tissues in the culture tank 200 is transferred to the seed tank 100, and when the liquid level detector detects that the liquid level of the seed tank 100 or the culture tank 200 reaches the preset seed returning liquid level, the tank bottom valve 110 and the seed returning valve 910 are closed; when the reseeding is completed, the reseeding line 900 needs to be cleaned and sterilized again.

And (3) discharging: after the seed returning operation is completed, the plant tissues remained in the culture tank 200 are subjected to 2-level culture, and when the production length of the plant tissues reaches the preset length, the plant tissues are sheared by the shearing device 3 so as to reduce the size of the plant tissues; simultaneously, the steam pipeline 500 is utilized to sterilize the tank bottom valve 110, the seed returning pipeline 900, the five-way valve 320 and the discharging pipeline 600, and then the tank bottom valve 110 at the bottom of the culture tank 200, the five-way valve 320 and the discharging pipeline 600 on the seed returning pipeline 900 are opened to discharge the mature plant tissues to the next process.

In another embodiment, as shown in fig. 20 and 21, the seed tank 100 and the culture tank 200 further comprise a feeding device 7 for injecting nutrients into the tank body, and the feeding device 7 comprises a feeding box 130, an acid feeding box 140, an alkali feeding box 150 and a defoaming liquid feeding box 160, and the four sets of boxes are connected with the corresponding four sets of structures through hoses.

The replenishing tank 130 is used for replenishing the culture solution in the tank; the acid replenishing tank 140 is used for replenishing acid liquor in the tank to maintain the pH value; the alkali replenishing box 150 is used for replenishing alkali liquor in the tank to maintain the pH value; the defoaming liquid replenishing tank 160 is used for replenishing defoaming liquid into the tank to eliminate foam generated by the in-tank culture.

According to the conditions of the residual solution in the four groups of tanks or the conditions of the culture solution, the acid solution, the alkaline solution and the defoaming solution which are needed to be supplemented in the tank body, the material supplementing device 7 opens the corresponding pneumatic valve and supplements the corresponding solution to the tank body, or adjusts the acid, the alkalinity or the defoaming solution of the culture solution. The feeding process can be manually controlled or can be automatically controlled to supplement corresponding culture solution according to the pH value detected by the acid supplementing tank 140 and the alkali supplementing tank 150, the foam generating condition in the tank body detected by the foam supplementing liquid tank 160, and the like, and the culture solution needed in the tank body is supplemented in stages.

The feeding device 7 is fixed above the outer side wall of the tank body of the bioreactor, and the feeding device 7 comprises a feeding structure 13, an acid supplementing structure 14, an alkali supplementing structure 15 and a defoaming liquid supplementing structure 16 which are sequentially and transversely arranged. The material supplementing device 7 is provided with a material supplementing air inlet pipe 131, an acid supplementing air inlet pipe 141, an alkali supplementing air inlet pipe 151 and a defoaming liquid supplementing air inlet pipe 161 which are corresponding to each other, the four groups of air inlet pipes are connected with the air inlet branch A110, and the material supplementing device further comprises a material supplementing air outlet pipe 132, an acid supplementing air outlet pipe 142, an alkali supplementing air outlet pipe 152 and a defoaming liquid supplementing air outlet pipe 162 which are connected with the steam supply main pipe B100, and the interior of the four groups of structures is disinfected through a high-temperature steam pipe.

The feeding structure 13 further comprises a feeding air inlet valve 133 and a feeding air outlet valve 134; the acid supplementing structure 14 also comprises an acid supplementing air inlet valve 143 and an acid supplementing air outlet valve 144; the alkali supplementing structure 15 further comprises an alkali supplementing air inlet valve 153 and an alkali supplementing air outlet valve 154; the defoaming liquid replenishing structure 16 further includes a defoaming liquid replenishing intake valve 163 and a defoaming liquid replenishing exhaust valve 164.

One end of the feeding hose 105 is connected with the feeding structure 13, and the other end is connected with the feeding box 130. One end of the acid supplementing hose 106 is connected with the acid supplementing structure 14, and the other end is connected with the acid supplementing box 140. One end of the alkali supplementing hose 107 is connected with the alkali supplementing structure 15, the other end is connected with the alkali supplementing box 150, one end of the defoaming liquid supplementing hose 108 is connected with the defoaming liquid supplementing structure 16, and the other end is connected with the defoaming liquid supplementing box 160.

The acid make-up pneumatic valve 114 is arranged on the acid make-up hose 106, the alkali make-up pneumatic valve 115 is arranged on the alkali make-up hose 107, and the defoaming liquid make-up pneumatic valve 116 is arranged on the defoaming liquid make-up hose 108.

Specifically, the feeding device 7 includes a feeding structure 13, and the feeding structure 13 includes a feeding pneumatic valve 113, a feeding air inlet pipe 131, a feeding air outlet pipe 132, a feeding air inlet valve 133, a feeding air outlet valve 134, a feeding hose 105, and a feeding box 130. A feed pneumatic valve 113 is provided on the feed hose 105 for controlling the opening and closing of the feed line. One end of the material supplementing hose 105 is connected with the material supplementing structure 13, and the other end is connected with the material supplementing box 130. One end of the feeding structure 13 is connected with a feeding air inlet pipe 131, the other end is connected with a feeding air outlet pipe 132, a feeding air inlet valve 133 is arranged on the feeding air inlet pipe 131, and a feeding air outlet valve 134 is arranged on the feeding air outlet pipe 132. The feed air inlet pipe 131 is connected to the steam supply main pipe B100, and the feed air outlet pipe 132 is connected to the heat drain pipe F2, for discharging high-temperature steam for sterilization.

The connection mode of the acid supplementing structure 14, the alkali supplementing structure 15, the defoaming liquid supplementing structure 16 and the pipeline is the same as the connection mode of the material supplementing structure 13 and the pipeline, and the connection provides a more convenient food injection mode and realizes the systematic control of the food injection technology into the tank body.

The acid supplementing structure 14 is used for supplementing an acidic solution required in the tank body, and when the pH value of the culture solution in the tank body is less than 7, the solution is acidic, and when the acidity value of the culture solution is too high, the growth of plant tissues is affected, so that the propagation of the plant tissues is hindered, the quantity is reduced or the growth is stopped, and therefore, alkali supplementing is required according to the pH value in the tank body.

The alkaline supplementing structure 15 is used for supplementing alkaline solution required in the tank body, and when the pH value of the culture solution in the tank body is more than 7, the solution is alkaline, and when the alkaline value of the culture solution is too high, the growth of plant tissues can be affected, so that acid supplementing is required according to the pH value in the tank body. The pH value of the culture solution in the tank should be controlled within a proper range.

The foam-supplementing liquid-removing structure 16 is characterized in that liquid in the tank body can drive plant tissues to vertically turn up and down, the liquid generates bubbles, the bubbles enable the concentration of the culture liquid to change, the growth of the plant tissues can be affected when the bubbles are excessive, a value X is preset for the standard height of the bubbles in the tank body, the foam-supplementing liquid-removing pneumatic valve 116 is opened when the generated bubbles exceed the preset value X, and foam-supplementing liquid in the tank body is supplemented as required to eliminate or reduce the bubbles on the liquid surface of the culture liquid.

The waste steam in the tank body is discharged to the exhaust pipeline A170 through the exhaust device, and the steam in the exhaust pipeline A170 is filtered by the gas filter A180 and then is discharged to the air.

In another embodiment, as shown in fig. 22, the temperature control layer 9 has an opening, a detecting device 8 is disposed at the opening, and the detecting device 8 extends into the tank.

The temperature control layer 9 is provided with an opening, and the position of the detection device 8 is avoided. The sealing material ageing, deformation or failure easily caused by high temperature are avoided, the damage of the sealing structure caused by high temperature is prevented, the replacement maintenance frequency and cost are reduced, the leakage of the heat transfer medium can be effectively prevented, the risks of accidents and disasters are reduced, and the safety of a workplace is improved. The detection device 8 is placed in the opening, so that the device can be maintained and operated more conveniently, compared with the device which directly passes through the temperature control layer 9, the detection device only needs to be operated and adjusted at the opening, the process of disassembling and reinstalling the temperature control layer 9 is avoided, and the workload and the time consumption are reduced. And the temperature control in the tank body is not accurate due to the fact that heat transmission at the sealing position is different from that at other positions, and the growth speed of plant tissues is influenced.

The detection device 8 comprises a temperature sensor, a pH detection device, a dissolved oxygen amount detection device, a sampling device, a density detector, a liquid level detector, a pressure detector and the like, and the detection device 8 is circumferentially arranged on the tank body at the opening at intervals.

The detection device 8 is directly arranged on the tank wall, can monitor temperature change, nutrition parameters and the like in the tank body in real time, is beneficial to timely adjusting nutrition supply in the tank body, ensures that plant tissues normally grow under the condition of obtaining sufficient nutrition support, improves the yield and the quality, can ensure that the temperature is kept stable in a proper range, and avoids negative influence of temperature fluctuation on the growth of the plant tissues.

The detection device 8 is positioned at the middle part of the culture solution and extends inwards for about ten centimeters, and the shearing device 3 and the gas distributor 40 can enable the culture solution to form a vortex which rolls up and down, so that part of the culture solution detected by the detection device 8 is the most uniformly mixed part in the culture solution, the accuracy of the detection result is ensured, and the culture efficiency is improved.

In the present invention, each valve is connected to a hose capable of providing steam sterilization, and sterilization treatment is performed before each valve is used, except for valves for discharging exhaust gas and waste liquid.

The control cabinet is positioned at one end of the length direction of the supporting frame, is close to the support extending from the supporting frame to the width direction, has a certain distance with the bioreactor and is used for controlling the effective operation of each component and realizing the automatic control of the whole device.

The control cabinet is provided with a display window which can display all parameters detected by the device; the device is also provided with an operation interface, and can adjust setting parameters or control the opening and closing of each component according to the needs.

In another embodiment, a control method of an industrial automation bioreactor apparatus is provided, applied to the industrial automation bioreactor apparatus as described above, the method comprising:

Further, the gas distributor is controlled to be opened, and air with a certain pressure is provided for the bioreactor, so that the culture solution and plant tissues in the bioreactor circularly move along the inner parabolic track which is close to the inner wall of the bioreactor and descends in the area close to the central axis.

Or controlling to open the gas distributor, and providing air with a certain pressure into the bioreactor, so that the culture solution and plant tissues in the bioreactor can rise along the area close to the central axis and can circularly move along the track of the outer paraboloid which is close to the inner wall of the bioreactor and descends.

Further, the shearing device is controlled to be opened, and the plant tissues in the descending process along the track approaching to the paraboloid are sucked into the shearing device by the vortex suction force generated by the rotation of the cutter and sheared.

Further, adjacent second blades connected by a frame body outside the first blades in the shearing device are controlled to rotate relative to the first blades which are arranged in a pasting mode, so that cuts formed by the relative rotation of the first blades and the second blades are opened and closed simultaneously, and plant tissues in the descending process are sheared into fixed-length small sections.

The foregoing description is only illustrative of the preferred embodiment of the present invention, and is not to be construed as limiting the invention, but is to be construed as limiting the invention to any simple modification, equivalent variation and variation of the above embodiments according to the technical matter of the present invention without departing from the scope of the invention.

Claims

1. An industrial automation bioreactor device, characterized by comprising a bioreactor with a conical bottom wall (11), wherein a gas distributor (40) and a shearing device (3) arranged above the gas distributor (40) are arranged on the bottom wall (11);

one end of an aeration part (42) of the gas distributor (40) is provided with a distance from the bottom wall (11), the other end of the aeration part is tangential to or intersects with the central line of the bioreactor, the gas provided by the aeration part (42) enables culture solution and plant tissues in the bioreactor to ascend along a region close to the central line, then to diffuse to the periphery and circularly move along an outer parabolic track close to the inner wall of the bioreactor, and certain force is applied to the plant tissues in the ascending and descending processes along the track close to the parabolic track so that the plant tissues are basically regularly arranged and flow along the length direction;

the shearing device (3) is vertically arranged on the bottom wall (11) and comprises a rotatable cutter, a shearing plane where the cutter is located and a track which is close to the falling of the paraboloid form are acute angles, and the culture solution guides plant tissues which are regularly arranged into the cutter in the falling process along the track which is close to the paraboloid form.

2. An industrial automation bioreactor according to claim 1, wherein the cutting knife comprises two knives arranged vertically and shearing a substantial portion of the plant tissue to the size of the space between the two knives.

3. An industrial automation bioreactor according to claim 2, characterized in that the double knife comprises a first blade (323) and a second blade (324) attached to the first blade (323), the second blade (324) rotating relative to the first blade (323) to form a continuously open and shut incision.

4. An industrial automation bioreactor apparatus according to claim 3, wherein the ends of the first blade (323) and the second blade (324) are respectively bent toward the side where the slit is formed to form a substantially annular slit.

5. An industrial automation bioreactor according to claim 4, wherein the ends of the upper and lower, distributed second blades (324) are connected by a frame covering the outside of the first blades (323).

6. An industrial automation bioreactor according to any one of claims 1-5, characterized in that the gas distributor (40) is alternatively connected to the steam supply main (B100) and the gas supply main (a 100) for introducing high temperature steam for sterilization into the seed tank (100) during the pre-cultivation period or introducing gas for cultivation into the seed tank (100) during the cultivation period.

7. An industrial automation bioreactor apparatus according to claim 6, characterized in that the aeration section (42) of the gas distributor (40) provides micro-bubbles into the culture liquid of the bioreactor.

8. An industrial automation bioreactor according to claim 7, wherein the aeration portion (42) has an extended length, a long column shape, a hollow interior for air inlet and a hollow wall with micropores for air outlet.

9. A method of controlling an industrial automation bioreactor apparatus as claimed in any one of claims 1 to 8, the method comprising:

10. A method of controlling an industrial automation bioreactor apparatus according to claim 9, wherein the method comprises: and controlling adjacent second blades connected by a frame body outside the first blades in the shearing device to rotate relative to the first blades which are respectively arranged in a pasting way, so that the incisions formed by the relative rotation of the first blades and the second blades are opened and closed simultaneously, and the plant tissues in the descending process are sheared into fixed-length small sections.