CN111718852B - Automatic change algae and separate fungus culture apparatus - Google Patents
Automatic change algae and separate fungus culture apparatus Download PDFInfo
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- CN111718852B CN111718852B CN202010649008.6A CN202010649008A CN111718852B CN 111718852 B CN111718852 B CN 111718852B CN 202010649008 A CN202010649008 A CN 202010649008A CN 111718852 B CN111718852 B CN 111718852B
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Abstract
The invention discloses an automatic algae isolation culture device, which comprises an algae culture control device and a transparent culture device; the algae culture control device comprises a water inlet valve, an electric three-way valve, a water pump, a thermostat and an ejector which are communicated through a pipeline, wherein the ejector is connected with the transparent culture device through a liquid inlet pipe; the ejector is also connected with a gas alga input device, the gas alga input device comprises an alga seed input device and a gas input device, and the alga seed input device comprises an alga seed input interface and a first check valve which are connected through pipelines; the gas input device comprises an air filter, an ozone machine, an air compressor and a second check valve which are connected through pipelines; the transparent culture device is connected with the electric three-way valve through a water return pipe. The invention adopts the algae cultivation control device to realize the input of algae species and the continuous automatic circulation of algae growth environment and water body in the transparent cultivation device, and realizes the cultivation of algae in a whole sterile and automatic way and the sustainable large-scale commercial application.
Description
Technical Field
The invention relates to the technical field of algae cultivation, in particular to an automatic algae bacteria-isolation cultivation device.
Background
Current algae basic culture mode:
pure culture and Single culture
1. Pure culture, i.e., sterile culture, refers to culture performed under conditions that exclude all organisms including bacteria. The pure culture requires facilities such as a sterile room, a sterilizing pot, an ultra-clean workbench and the like, and the container, the tool, the culture solution and the like are thoroughly sterilized, so that the operation is strict, the culture success rate is high, and the method is an indispensable link for biological science research.
2. One mode of culture that does not exclude the presence of bacteria in productive culture, but differs from pure culture, is called single culture.
(II) one-time culture, continuous culture and semi-continuous culture
1. Culturing in various containers at one time, preparing culture solution, inoculating small amount of algae, and culturing under appropriate environment
After culturing for a period of time (generally 5-7 days), the algae cells grow and propagate to reach higher density, and are harvested at one time. Disposable culture is the most common method for microalgae culture.
2. Semi-continuous culture is to harvest a part of algae liquid every day and supplement the same amount of culture liquid to continue culture when the cultured algae cells reach or approach the harvest density on the basis of one-time culture. Semi-continuous culture is also a common culture method for microalgae bait production, and the daily harvest is determined according to the needs of seedling culture.
From the background art, it is clear that there is no device or method capable of automatically cultivating algae at present, and the inventor of the present application has conducted intensive research on the above problems, and how to implement automatic cultivation of algae with bacteria isolated is the subject of research by those skilled in the art.
Disclosure of Invention
The invention provides an automatic algae isolation culture device which has large single culture quantity of algae, high survival rate and large-scale commercial culture value.
An automatic algae isolation culture device comprises an algae culture control device and a transparent culture device; the algae culture control device comprises a water inlet valve, an electric three-way valve, a water pump, a thermostat and an ejector which are communicated through a pipeline, wherein the ejector is connected with the transparent culture device through a liquid inlet pipe; the ejector is also connected with a gas alga input device, the gas alga input device comprises an alga seed input device and a gas input device, and the alga seed input device comprises an alga seed input interface and a first check valve which are connected through pipelines; the gas input device comprises an air filter, an ozone machine, an air compressor and a second check valve which are connected through pipelines; the transparent culture device is connected with the electric three-way valve through a water return pipe.
The transparent culture device comprises more than two mutually connected transparent pipe sections, and the more than two mutually connected transparent pipe sections are fastened and connected through flange plate matched bolts.
The outer side of the transparent culture device is respectively connected with an exhaust valve, a vacuum valve and a water level sensor; the outside of the water return pipe is connected with a temperature sensor; the water level sensor and the temperature sensor are both connected with the circuit control board.
The transparent culture device is internally provided with an integrated algae seed input, circulation culture and cleaning system, and the algae seeds are transfused. The system for entering, circulating culturing and cleaning comprises a algae seed input pipe rotary driving device positioned outside the transparent culturing device and a algae seed output pipe positioned outside the transparent culturing device
The algae seed input pipe inside the transparent culture device, the algae seed input pipe outlet is located the transparent culture device border, and algae seed input pipe outer wall is equipped with the cleaning nozzle.
The algae seed input pipe rotary driving device comprises a fixed connecting pipe connected with one end of a liquid inlet pipe, the other end of the fixed connecting pipe is connected with a bearing in an adapter, another independent bearing is arranged in the adapter, the independent bearing is connected with one end of a tubular rotary joint with a key pin surface, the periphery of the tubular rotary joint is connected with a driven gear, the driven gear is meshed with a driving gear on a connecting motor, the other end of the tubular rotary joint is connected with a tubular driving joint, and the other end of the tubular driving joint is connected with an algae seed input pipe.
The periphery of the tubular driving joint is respectively sleeved with a first flange plate, a fixed sealing ring, a rotary sealing ring and a second flange plate, and the first flange plate and the second flange plate are clamped and connected with the outer wall of the transparent culture device through a screw rod; the periphery of the rotary sealing ring is sleeved with a spring.
A plane bearing is arranged between the first flange plate and the fixed sealing ring, and a first plane bearing is arranged between the rotary sealing ring and the second flange plate.
The fixed sealing ring and the rotary sealing ring are internally provided with metal sealing rings, and the two metal sealing rings are mutually attached and sealed.
The ejector is of a three-way pipe structure, the straight line part of the three-way pipe structure is respectively connected with a constant temperature machine and a liquid inlet pipe, the branch pipe part of the three-way pipe structure is connected with an injection joint, a sealing ring is arranged in the injection joint, a sealing ball is matched with an inner hole of the sealing ring in a sealing mode, and the end part of the sealing ball is connected with a sealing spring arranged in the branch pipe.
A PH value sensor is connected to a pipeline between the thermostat and the water pump; and a flowmeter, an electromagnetic valve and an algae discharge tap are connected between the thermostat and the ejector through pipelines.
The outer periphery of the algae seed input pipe is connected with a support bearing, the outer periphery of the support bearing is connected with more than three support rods which are uniformly distributed, the end parts of the support rods are connected with support ejector rods through adjusting springs, and the support ejector rods are propped against the inner wall of the transparent pipe section.
From the above embodiments, it is apparent that the present invention has the following advantages:
1. the invention adopts the algae cultivation control device to realize the input of algae species and the continuous automatic circulation of algae growth environment and water body in the transparent cultivation device, and realizes the cultivation of algae in a whole sterile and automatic way and the sustainable large-scale commercial application.
2. The invention greatly increases the quantity of algae cultivated in a single time through more than two transparent pipe sections which are connected with each other, and improves the economic value of algae cultivation.
3. The invention solves the problems of automatic supply and circulation culture of algae seeds and cleaning after algae attach to the wall of the transparent culture device after long-term culture by arranging the integrated algae seed input, circulation culture and cleaning system in the transparent culture device.
Drawings
FIG. 1 is a schematic diagram of the principle and structure of an automatic algae bacteria-isolation culture device;
FIG. 2 is a schematic diagram showing a perspective structure of a rotary driving device for algae seed input pipes;
FIG. 3 is a schematic perspective view of the frying open structure of the injector according to the present invention.
Description of the embodiments
An automatic algae bacteria-isolation culture device as shown in fig. 1-3 comprises an algae culture control device and a transparent culture device, wherein the algae culture control device comprises a water inlet valve, an electric three-way valve, a water pump, a thermostat and an ejector which are communicated through a pipeline, and the ejector is connected with the transparent culture device through a liquid inlet pipe 30. The algae cultivation control apparatus is entirely located in the control box 22.
The structure optimization ejector 23 is also connected with a gas algae input device, wherein the gas algae input device comprises an algae seed input device and a gas input device, and the algae seed input device comprises an algae seed input interface and a first check valve which are connected through pipelines to realize one-way algae seed input performance; the gas input device comprises an air filter, an ozone machine, an air compressor and a second check valve which are connected through pipelines, the one-way input performance of the culture gas after sterilization treatment is realized, algae seeds and the gas are mixed and then are mixed into the culture environment in the transparent culture device, the oxygen content of the water body is continuously increased, the culture speed of the algae seeds is improved, and the death of the algae is avoided. As the automatic air pressure regulating structure of the transparent culture device, the outer side of the transparent culture device is respectively connected with an exhaust valve, a vacuum valve and a water level sensor, when the transparent culture device is used for feeding water, the exhaust valve is exhausted, when the algae is placed in the culture, the vacuum valve is automatically opened to avoid the occurrence of negative pressure, so that the algae cannot be placed, and the water level sensor is used for controlling the water level of the transparent culture device in real time to supplement water in time. The water entering from the water inlet valve is filtered by a filter, and the filter is connected with a drain valve. The transparent culture device is connected with an electric three-way valve through a water return pipe 29, and then liquid in the transparent culture device is output by driving a water pump to realize the internal circulation of algae culture water. The outside of the water return pipe 29 is connected with a temperature sensor; the water level sensor and the temperature sensor are both connected with the circuit control board.
The transparent culture device comprises more than two interconnected transparent tube sections 3, the more than two interconnected transparent tube sections 3 are fastened and connected through the flange 4 and the bolt 9, and the capacity of the transparent culture device is increased while water leakage is avoided. The transparent culture device is internally provided with an integrated algae seed input, circulation culture and cleaning system, the algae seed input, circulation culture and cleaning system comprises an algae seed input pipe rotary driving device positioned at the outer side of the transparent culture device and an algae seed input pipe 1 positioned in the transparent culture device, an outlet of the algae seed input pipe 1 is positioned at the edge of the transparent culture device, and liquid in the whole transparent culture device is driven by liquid discharged from the algae seed input pipe 1 to flow so as to prevent algae precipitation, thereby being more beneficial to algae culture.
The rotary driving device 8 for the algae seed input pipe, which is preferable as a structure, comprises a fixed connecting pipe 18 connected with one end of a liquid inlet pipe 30, wherein the other end of the fixed connecting pipe 18 is connected with a bearing in an adapter 17, the adapter 17 is internally provided with another independent bearing, the independent bearing is connected with one end of a tubular rotary joint 16 with a key pin surface 19, the periphery of the tubular rotary joint 16 is connected with a driven gear 6, the driven gear 6 is meshed with a driving gear connected with a motor 7, the other end of the tubular rotary joint 16 is connected with a tubular driving joint 31, the other end of the tubular driving joint 31 is connected with the algae seed input pipe 1, and the outer wall of the algae seed input pipe 1 is provided with a cleaning nozzle 2. The algae seed input pipe rotary driving device 8 realizes the rotary performance of the algae seed input pipe 1, the output liquid has rotary flow velocity to further prevent algae precipitation, the cleaning nozzle 2 utilizes the high pressure of the nozzle to achieve the cleaning performance of the transparent culture device to prevent algae from adhering, and the algae seed rotary driving device can also be used as an output port of algae seed liquid.
As the structure, preferably, the outer periphery of the algae seed input pipe 1 is connected with a supporting bearing 34, the outer periphery of the supporting bearing 34 is connected with more than three supporting rods 33 which are uniformly distributed, the end parts of the supporting rods 33 are connected with supporting ejector rods 31 through adjusting springs 32, the supporting ejector rods 31 are propped against the inner wall of the transparent pipe section 3, and the supporting structure ensures that the algae seed input pipe 1 which is connected with each other can not bend due to overlong and maintain the balance performance of a rotating state through the elastic deformation of the adjusting springs 32.
In order to further improve the sealing performance of the connection, the outer periphery of the tubular driving joint 31 is respectively sleeved with a first flange plate 20, a fixed sealing ring 15, a rotary sealing ring 21 and a second flange plate 11, and the first flange plate 20 and the second flange plate 11 are clamped and connected with the outer wall 10 of the transparent culture device through a screw rod. In order to further prevent the clamping between the first flange 20 and the second flange 11 against the outer wall 10 of the transparent culture device from loosening, a spring 13 is sleeved on the outer periphery of the rotary sealing ring 21.
As a structure, a planar bearing is preferably arranged between the first flange plate 20 and the fixed sealing ring 15, and a first planar bearing 12 is arranged between the rotary sealing ring 21 and the second flange plate 11, so that the friction resistance of the tubular driving joint 31 in the rotation process is reduced. The fixed sealing ring 15 and the rotary sealing ring 21 are internally provided with the metal sealing rings 14, and the two metal sealing rings 14 are mutually attached and sealed, so that the sealing of a mechanical face and a surface is realized, the overall sealing effect is improved, and water leakage is prevented. The axial cross section of the rotary sealing ring 21 is in a circular arc structure, and when the rotary sealing ring 21 is compressed, the inner wall can generate a pressing sealing force on the outer wall of the tubular driving joint 31, so that the sealing effect is further improved.
In order to form a siphon effect, the ejector 23 is of a three-way pipe structure, the straight line part of the three-way pipe structure is respectively connected with a thermostat and a liquid inlet pipe 30, the branch pipe part of the three-way pipe structure is connected with an injection joint 28, the inside of the injection joint 28 is provided with a sealing ring 26, the inner hole of the sealing ring 26 is hermetically matched with a sealing ball 25, the end part of the sealing ball 25 is connected with a sealing spring 24 arranged inside the branch pipe to realize unidirectional sealing, the diameter of the straight line pipe body is reduced in the liquid flowing process in the straight line part of the three-way pipe structure, and then the gas and algae seeds in the siphon effect suction and algae input device are formed by amplification.
The structure is preferably that a PH value sensor is connected on a pipeline between the thermostat and the water pump to control the inflow of the water inlet valve; the constant temperature machine and the ejector are connected with a flowmeter, an electromagnetic valve and an algae discharge tap through pipelines, so that automatic discharge of the cultured algae is realized.
Claims (7)
1. An automatic change algae and separate fungus culture apparatus which characterized in that: the automatic algae isolation culture device comprises an algae culture control device and a transparent culture device; the algae culture control device comprises a water inlet valve, an electric three-way valve, a water pump, a thermostat and an ejector which are communicated through a pipeline, wherein the ejector is connected with the transparent culture device through a liquid inlet pipe (30); the ejector is also connected with a gas alga input device, the gas alga input device comprises an alga seed input device and a gas input device, and the alga seed input device comprises an alga seed input interface and a first check valve which are connected through pipelines; the gas input device comprises an air filter, an ozone machine, an air compressor and a second check valve which are connected through pipelines; the transparent culture device is connected with an electric three-way valve through a water return pipe (29), and comprises more than two mutually connected transparent pipe sections (3), and the more than two mutually connected transparent pipe sections (3) are tightly connected through a flange plate (4) and a bolt (9); the transparent culture device is internally provided with an integrated algae seed input, circulation culture and cleaning system, the algae seed input, circulation culture and cleaning system comprises an algae seed input pipe rotary driving device positioned at the outer side of the transparent culture device and an algae seed input pipe (1) positioned in the transparent culture device, an outlet of the algae seed input pipe (1) is positioned at the edge of the transparent culture device, and a cleaning nozzle (2) is arranged on the outer wall of the algae seed input pipe (1); the algae seed input pipe rotary driving device (8) comprises a fixed connecting pipe (18) connected with one end of a liquid inlet pipe (30), one end of the fixed connecting pipe (18) is connected with a bearing in an adapter (17), another independent bearing is arranged in the adapter (17), the independent bearing is connected with one end of a tubular rotary joint (16) with a key pin surface (19), the periphery of the tubular rotary joint (16) is connected with a driven gear (6), the driven gear (6) is meshed with a driving gear on a connecting motor (7), the other end of the tubular rotary joint (16) is connected with a tubular driving joint (31), and the other end of the tubular driving joint (31) is connected with the algae seed input pipe (1).
2. An automated algae isolation culture apparatus according to claim 1, wherein: the outer side of the transparent culture device is respectively connected with an exhaust valve, a vacuum valve and a water level sensor; the outside of the water return pipe (29) is connected with a temperature sensor; the water level sensor and the temperature sensor are both connected with the circuit control board.
3. An automated algae isolation culture apparatus according to claim 1, wherein: the periphery of the tubular driving joint (31) is respectively sleeved with a first flange plate (20), a fixed sealing ring (15), a rotary sealing ring (21) and a second flange plate (11), and the first flange plate (20) and the second flange plate (11) are connected with the outer wall of the transparent culture device through screw clamping; the periphery of the rotary sealing ring (21) is sleeved with a spring (13).
4. An automated algae isolation culture apparatus according to claim 3, wherein: a plane bearing is arranged between the first flange plate (20) and the fixed sealing ring (15), and a first plane bearing (12) is arranged between the rotary sealing ring (21) and the second flange plate (11).
5. An automated algae isolation culture apparatus according to claim 3, wherein: the inner parts of the fixed sealing ring (15) and the rotary sealing ring (21) are respectively provided with a metal sealing ring (14), and the two metal sealing rings (14) are mutually attached and sealed.
6. An automated algae isolation culture apparatus according to claim 1, wherein: the ejector (23) is of a three-way pipe structure, a linear part of the three-way pipe structure is respectively connected with a thermostat and a liquid inlet pipe (30), a branch pipe part of the three-way pipe structure is connected with an injection joint (28), a sealing ring (26) is arranged in the injection joint (28), a sealing ball (25) is closely matched with an inner hole of the sealing ring (26), and the end part of the sealing ball (25) is connected with a sealing spring (24) arranged in the branch pipe.
7. An automated algae isolation culture apparatus according to claim 1, wherein: the algae seed input pipe (1) periphery is connected with supporting bearing (34), and supporting bearing (34) periphery is connected with three or more evenly distributed's bracing piece (33), and bracing piece (33) tip is connected with the support ejector pin through adjusting spring (32), and the support ejector pin top connects transparent pipe section (3) inner wall.
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