CN117281045B

CN117281045B - Temperature regulating system of plant tissue culture tank

Info

Publication number: CN117281045B
Application number: CN202311560711.XA
Authority: CN
Inventors: 刘冰; 刘金坤; 张洪喜; 张明臣; 高秀君
Original assignee: Shanghai Kedilian Technology Co ltd
Current assignee: Shanghai Kedilian Technology Co ltd
Priority date: 2023-11-22
Filing date: 2023-11-22
Publication date: 2024-02-13
Anticipated expiration: 2043-11-22
Also published as: CN117281045A

Abstract

The invention discloses a temperature regulating system of a plant tissue culture tank, which comprises a supply unit, a temperature control layer coated on the outer surface of the plant tissue culture tank, a conveying unit communicated with the supply unit and the temperature control layer, and a temperature regulating water tank communicated with the supply unit, wherein the supply unit carries various liquids with different temperatures and leads in and out the temperature control layer and/or the temperature regulating water tank through the conveying unit. According to the invention, the temperature regulating water tank is additionally arranged in the temperature regulating system, and the temperature regulating water tank and the temperature control layer are both connected to the supply unit, so that the temperature regulating water tank and the temperature control layer form a loop connected in parallel with the supply unit, the temperature regulating water tank and the temperature control layer can circulate heat exchange medium with the supply unit at the same time, and the temperature regulating water tank and the temperature control layer can be connected in series by the supply unit to form a temperature regulating loop, so that the temperature regulating water tank directly circulates with the temperature control layer, the temperature regulating loop can be used for independently regulating the temperature of the plant tissue culture tank when a supply source of the temperature regulating system is damaged and maintained, and the fault tolerance of the temperature regulating system is improved.

Description

Temperature regulating system of plant tissue culture tank

Technical Field

The invention belongs to the technical field of plant tissue culture devices, and particularly relates to a temperature regulating system of a plant tissue culture tank.

Background

Technological staff develop a method and a culture device for large-scale cultivation by utilizing isolated tissues or cells of plants. Callus and adventitious roots are induced by stripping roots, stems, leaves, etc. of plants, and then mass production is performed in a culture apparatus using these callus and adventitious roots.

In order to conveniently regulate the temperature of the culture solution in the culture device, the outer side of the culture device is coated with a temperature control layer which can perform heat transfer with the culture device, and liquid mediums with different temperatures can be introduced into the temperature control layer for regulating and maintaining the temperature of the culture solution. Typically, the temperature control layers of the culture apparatus are directly connected to a supply of liquid medium via a pipeline, and a plurality of temperature control layers are connected in parallel with one supply to form a circulation system capable of maintaining a plurality of culture apparatuses. However, this circulation system is extremely poor in fault tolerance, and when the supply source is damaged or overhauled, all the culture devices in the circulation system lose the ability to regulate the temperature of the culture solution, causing a great loss.

The invention patent of China with the application number of 202310491603.5 discloses a temperature-regulating fermentation tank for CHO cell culture, belongs to the technical field of cell culture, and solves the problems of inconvenient temperature regulation, low fermentation preparation efficiency of CHO cells and the like of the conventional fermentation tank. Including by preceding two prefabricated jar subassemblies, biax fermentation cylinder and the receiving tank that communicate in proper order and set up, the left side of biax fermentation cylinder sets gradually air compressor machine and seed tank, and the front side of seed tank sets up the steam tank, and the both sides of the prefabricated jar subassembly of second all set up the reposition of redundant personnel subassembly, and the steam tank is connected with the reposition of redundant personnel subassembly of homonymy, and the right side of biax fermentation cylinder sets up the cooler, and the front side of cooler sets up hot water circulation mechanism, hot water circulation mechanism and cooler all with the reposition of redundant personnel subassembly intercommunication of homonymy, seed tank and cooler all with biax fermentation cylinder intercommunication.

However, the hot water circulation mechanisms are connected with the fermentation tanks in a one-to-one correspondence manner, and belong to closed-loop heat exchange and temperature adjustment circuits, and heat exchange media can only be heated by the hot water circulation mechanisms and supplied to the fermentation tanks, so that the large-scale fermentation tanks occupy too large sites, and after the large-scale fermentation tanks are arranged, the temperature adjustment circuits of all the fermentation tanks are required to be combined and optimized so as to improve the heat exchange and temperature adjustment efficiency.

In view of this, the present invention has been made.

Disclosure of Invention

The invention aims to provide a temperature regulating system of plant tissue culture tanks, so as to realize the purposes of reducing equipment in a heat exchange loop corresponding to each plant tissue culture tank and ensuring that the heat exchange loop of each plant tissue culture tank can independently regulate temperature when the temperature regulating system is damaged and maintained, thereby improving the fault tolerance of the temperature regulating system.

In order to solve the technical problems, the invention adopts the basic conception of the technical scheme that:

the temperature regulating system of the plant tissue culture tank comprises a supply unit, a temperature control layer coated on the outer surface of the plant tissue culture tank, a conveying unit communicated with the supply unit and the temperature control layer, and a temperature regulating water tank communicated with the supply unit, wherein the supply unit carries various liquids with different temperatures and leads in and out of the temperature control layer and/or the temperature regulating water tank through the conveying unit;

The temperature-regulating water tank is provided with a water containing cavity, and the water containing cavity and the temperature-regulating layer are connected with the supply unit through the conveying unit to form a circulation loop; the conveying unit comprises a water inlet pipe and a water outlet pipe which are communicated with the supply unit and the water containing cavity;

the supply unit comprises a hot water supply main pipe and a hot water recovery main pipe which are arranged in parallel, the conveying unit comprises a hot water supply branch pipe and a hot water recovery branch pipe, one end of the hot water supply branch pipe is connected with the hot water supply main pipe, the other end of the hot water supply branch pipe is connected with the temperature control layer, one end of the hot water recovery branch pipe is connected with the temperature control layer, and the other end of the hot water recovery branch pipe is connected with the hot water recovery main pipe;

the water inlet pipe is connected with the hot water supply main pipe, the water outlet pipe is connected with the hot water recovery main pipe, and the water containing cavity and the temperature control layer are connected in series in the same loop;

the delivery unit comprises a liquid inlet pipe and a liquid outlet pipe which are communicated with the temperature-regulating water tank and the delivery unit and provided with a plurality of branches;

the temperature regulating system also comprises a heat exchange structure and a master control unit electrically connected with the pneumatic valves connected in series in each branch of the liquid inlet pipe, wherein the master control unit is used for controlling the proportion of the liquid inlet pipe to the chilled water and the high-temperature steam conveyed to the heat exchange structure of the temperature regulating water tank.

Further, the heat exchange structure is arranged against the water containing cavity.

Further, a water pump is arranged in the water inlet pipe in series and used for conveying the water in the water containing cavity to the temperature control layer.

Further, a branch pipe connected with the water pump in parallel is arranged on the water inlet pipe, and two ends of the branch pipe are respectively connected with a water inlet end and a water outlet end of the water pump communicated with the water inlet pipe.

Further, the supply unit comprises a cold row pipeline which is arranged in parallel with the chilled water supply main pipe,

the liquid inlet pipe comprises a chilled water liquid inlet pipe, the liquid outlet pipe comprises a chilled water liquid outlet pipe, one end of the chilled water liquid inlet pipe is connected with a chilled water supply main pipe, the other end of the chilled water liquid inlet pipe is connected with the heat exchange structure, and one end of the chilled water liquid outlet pipe is connected with the heat exchange structure, and the other end of the chilled water liquid outlet pipe is connected with a cold discharge pipeline.

Further, the supply unit comprises a heat exhaust pipe arranged in parallel with the steam supply main pipe,

the liquid inlet pipe comprises a steam liquid inlet pipe, the liquid outlet pipe comprises a steam liquid outlet pipe, one end of the steam liquid inlet pipe is connected with the steam supply main pipe, the other end of the steam liquid inlet pipe is connected with the heat exchange structure, and one end of the steam liquid outlet pipe is connected with the heat exchange structure, and the other end of the steam liquid outlet pipe is connected with the heat exhaust pipe.

Further, two parallel branches are arranged on the steam liquid inlet pipe, one branch is connected with a manual valve and a pneumatic valve in series, and the other branch is connected with a manual valve only in series and is used for manually controlling the on-off of the steam liquid inlet pipe when automatic control fails.

Further, the steam liquid outlet pipe is split into two pipelines at one end connected with the temperature-regulating water tank, one pipeline is connected with a one-way valve and a pneumatic valve in series, and the other pipeline is connected with the pneumatic valve only in series.

By adopting the technical scheme, compared with the prior art, the invention has the following beneficial effects.

1. The temperature adjusting system is additionally provided with the temperature adjusting water tank, and the temperature adjusting water tank and the temperature control layer are connected to the supply unit, so that the temperature adjusting water tank and the temperature control layer form a loop connected with the supply unit in parallel, the temperature adjusting water tank and the temperature control layer can circulate heat exchange medium with the supply unit at the same time, and the temperature adjusting water tank and the temperature control layer can be connected in series by the supply unit to form a temperature adjusting loop, so that the temperature adjusting water tank can circulate with the temperature control layer directly, the temperature adjusting loop can be used for independently adjusting the temperature of the plant tissue culture tank when a supply source of the temperature adjusting system is damaged and maintained, and the fault tolerance of the temperature adjusting system is improved.

2. The temperature-regulating water tank and the plant tissue culture tank are communicated with the supply unit in parallel, so that the supply unit can simultaneously convey hot water to the temperature-regulating water tank and the temperature-controlling layer, and the temperature-regulating water tank can convey hot water to the temperature-controlling layer, thereby adding a standby heat supply source for the temperature-regulating system, and ensuring that the plant tissue culture tank can normally maintain and regulate the temperature of nutrient solution in the plant tissue culture tank when the supply unit is damaged or maintained.

3. Through setting up heat transfer structure in the temperature adjusting water tank for the hot water in the temperature adjusting water tank keeps at the settlement temperature always when the supply unit is operated, thereby ensures that the temperature adjusting water tank can be immediately to the temperature control layer transport hot water when the supply unit stops supplying or maintaining, need not to wait for the water of temperature adjusting water tank to heat, guarantees that the reserve heat transfer circuit of temperature adjusting system can take over main heat transfer circuit to maintain the normal temperature of plant tissue culture jar fast.

The following describes the embodiments of the present invention in further detail with reference to the accompanying drawings.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention. It is evident that the drawings in the following description are only examples, from which other drawings can be obtained by a person skilled in the art without the inventive effort. In the drawings:

FIG. 1 is a schematic diagram of a tempering system for a plant tissue culture tank according to the present invention;

FIG. 2 is a schematic diagram of a piping structure of a continuous culture apparatus according to the present invention;

FIG. 3 is a schematic diagram showing a piping structure of another continuous culture apparatus according to the present invention;

FIG. 4 is a schematic illustration of an inlet structure of the present invention coupled to a plant tissue culture tank;

FIG. 5 is a schematic view of an air intake structure according to the present invention;

fig. 6 is a schematic cross-sectional view of an air intake structure according to the present invention.

Wherein: a100, a main air supply pipe; b100, a steam supply main pipe; c100, a chilled water recovery main pipe; d100, a cooling water recovery main pipe; e100, a hot water recovery main pipe; c200, a chilled water supply main pipe; e200, a hot water supply main pipe; d200, a cooling water supply main pipe; 200. a plant tissue culture tank; 201. a seed tank; 202. a fermentation tank; 300. a cold row pipeline; 400. a heat exhaust pipe; 410. a depressurization filter; 420. a discharge branch; c20, chilled water supply branch pipe; d20, cooling water supply branch pipes; e20, hot water supply branch pipes; c10, chilled water recycling branch pipes; d10, cooling water recycling branch pipes; e10, hot water recovery branch pipes; 1. a main pipeline; 2. dividing pipelines; 3. a continuous culture device; 4. a pneumatic valve; 5. a manual valve; 6. an input tube; 11. a tank wall; 40. an air intake structure; 41. an air inlet part; 42. an aeration section; 43. a conduit; 431. an air inlet section; 432. an air outlet section; 433. a transition section; 44. a seat plate; 45. quick release joint; 451. sealing grooves; 51. an interface; 52. stacking the pressing piece; 521. a through hole; 53. a hub kit; 531. a support ring; 532. a groove; 533. a connection hole; 54. a seal ring; a110, an air inlet branch; a120, a check valve; a130, a filtering device; a140, filtering and eliminating branches; a150, a tank elimination branch; a160, a standby branch; a170, an exhaust pipeline; a180, a gas filter; a1, a supply unit; a2, a conveying unit; 31. a temperature control layer; a3, a temperature-regulating water tank; a301, a water containing cavity; a21, a water inlet pipe; a211, a water pump; a212, branch pipes; a22, a water outlet pipe; a23, a heat exchange structure; a24, a liquid inlet pipe; a241, chilled water inlet pipe; a242, a steam liquid inlet pipe; a25, a liquid outlet pipe; a251, freezing the water outlet pipe; a252, a steam liquid outlet pipe.

It should be noted that these drawings and the written description are not intended to limit the scope of the inventive concept in any way, but to illustrate the inventive concept to those skilled in the art by referring to the specific embodiments.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions in the embodiments will be clearly and completely described with reference to the accompanying drawings in the embodiments of the present invention, and the following embodiments are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

In the description of the present invention, it should be noted that the azimuth or positional relationship indicated by the terms "inner", "outer", etc. are based on the azimuth or positional relationship shown in the drawings, and are merely for convenience of describing the present invention and simplifying the description, and are not indicative or implying that the apparatus or element in question must have a specific azimuth, be constructed and operated in a specific azimuth, and thus should not be construed as limiting the present invention.

In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "contacting," and "communicating" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.

Example 1

Referring to fig. 1 and 3, in an embodiment of the present invention, a temperature regulating system for a plant tissue culture tank 200 is described. The temperature regulating system comprises a supply unit A1, a temperature control layer 31 coated on the outer surface of the plant tissue culture tank 200 and a conveying unit A2 communicated with the supply unit A1 and the temperature control layer 31. The supply unit A1 carries a plurality of liquids having different temperatures, and introduces and removes the temperature control layer 31 via the transport unit A2. The supply unit A1 comprises a plurality of main pipelines 1 which are arranged in parallel, and each main pipeline 1 is used for respectively leading in and leading out different substances to the plant tissue culture tank 200 or the temperature control layer.

In particular, the tempering system further comprises a tempering tank A3 in communication with the supply unit A1. The temperature-adjusting water tank A3 is connected to the supply unit A1 in parallel with the plant tissue culture tank 200. The supply unit A1 is capable of carrying a plurality of liquids having different temperatures and introducing and discharging the liquid into and from the temperature-adjusting water tank A3 via the conveying unit A2. The water in the temperature-regulating water tank A3 circulates to the temperature-controlling layer to exchange heat with the plant tissue culture tank 200 and then flows back to the temperature-regulating water tank A3.

In this embodiment, the temperature-adjusting water tank A3 is added in the temperature-adjusting system, and the temperature-adjusting water tank A3 and the temperature-controlling layer 31 are both connected to the supply unit A1, so that the temperature-adjusting water tank A3 and the temperature-controlling layer 31 form a loop parallel to the supply unit A1, the temperature-adjusting water tank A3 and the temperature-controlling layer 31 can exchange heat medium with the supply unit A1 at the same time, and the temperature-adjusting water tank A3 and the temperature-controlling layer 31 can be connected in series by the supply unit A1 to form a temperature-adjusting loop, so that the temperature-adjusting water tank A3 can directly circulate with the temperature-controlling layer 31, and the temperature-adjusting loop can be used for independently adjusting the temperature of the plant tissue culture tank 200 when the supply source of the temperature-adjusting system is damaged and maintained, thereby improving the fault tolerance of the temperature-adjusting system.

Referring to fig. 1 and 3, in an embodiment of the present invention, a temperature regulating system for a plant tissue culture tank 200 is described. The temperature regulating system is additionally provided with a temperature regulating water tank A3 and a heat exchange structure A23.

Specifically, the temperature-adjusting water tank A3 is provided with a water containing cavity a301 for containing and heating the liquid that can be input into the temperature-controlling layer. In addition, the heat exchange structure A23 is arranged close to the water containing cavity A301 and can exchange heat with the liquid in the water containing cavity A301, so that a circulation loop capable of supplying heat to the temperature control layer is formed between the temperature adjusting water tank A3 and the temperature control layer.

Wherein, the water containing cavity a301 and the temperature control layer 31 are connected with the supply unit A1 through the conveying unit A2 to form a circulation loop. The conveying unit A2 comprises a water inlet pipe A21 and a water outlet pipe A22 which are communicated with the supply unit A1 and the water containing cavity A301, and the temperature of the plant tissue culture tank 200 is maintained by the temperature regulating water tank A3.

Further, the supply unit A1 includes a hot water supply main E200, a chilled water supply main C200, and a steam supply main B100. Each of which carries hot water, chilled water and high temperature steam.

The conveying unit A2 comprises a liquid inlet pipe A24 and a liquid outlet pipe A25 which are communicated with the temperature-regulating water tank A3 and the supply unit A1. The liquid inlet pipe A24 is provided with a plurality of branches, and can send chilled water and high-temperature steam into the temperature-regulating water tank A3 or the heat exchange structure A23.

The temperature regulating system further comprises a master control unit. The total control unit is electrically connected with the pneumatic valves connected in series in each branch of the liquid inlet pipe A24, and can adjust the flow ratio of the chilled water and the high-temperature steam to the temperature-adjusting water tank A3 and the heat exchange structure A23. Chilled water and high-temperature steam enter the temperature-regulating water tank A3 or the heat exchange structure A23 and can exchange heat with hot water in the water containing cavity A301, so that the hot water is ensured to meet the requirements of the plant tissue culture tank when being conveyed into the temperature-regulating layer 31. Even when the supply unit A1 cannot directly supply hot water to the temperature control layer 31, the hot water can be supplied to the temperature control layer 31 by the temperature control water tank A3, and the operation of the plant tissue culture tank 200 can be maintained.

In another embodiment, an electric heating structure can be arranged in the temperature-regulating water tank A3 to directly heat the liquid in the water containing cavity. Thus, when the hot water cannot be directly supplied from the hot water supply main pipe E200 to the temperature control layer 31, the hot water supply main pipe E200 and the hot water recovery main pipe can be controlled to directly communicate the temperature control layer 31 with the temperature control tank A3. Thus, a single circulation loop is formed between the temperature-adjusting water tank A3 and the temperature-controlling layer 31, and the temperature-adjusting water tank A3 can be utilized to directly convey hot water to the temperature-controlling layer 31, so that the requirement of the culture tank on the hot water temperature is ensured.

In particular, during normal operation of the supply unit A1, the temperature control layer 31 may be supplied with hot water directly supplied from the hot water supply main pipe or with hot water supplied from the temperature-adjusting water tank A3. At this time, the temperature of the hot water in the water containing cavity a201 can be further raised, and then the hot water is conveyed into the hot water supply main pipe, so that the hot water in the hot water supply main pipe can be subjected to temperature compensation, and the temperature of the culture tank can be adjusted and controlled more finely.

In this embodiment, the temperature-adjusting water tank A3 and the plant tissue culture tank 200 are connected in parallel to the supply unit A1, so that the supply unit A1 can simultaneously convey hot water to the temperature-adjusting water tank A3 and the temperature-controlling layer, and the temperature-adjusting water tank A3 can also convey hot water to the temperature-controlling layer, thereby adding a standby heat supply source to the temperature-adjusting system, and ensuring that the plant tissue culture tank 200 can normally maintain and adjust the temperature of the nutrient solution in the plant tissue culture tank 200 when the supply unit A1 is damaged or maintained.

Referring to fig. 1 and 3, in an embodiment of the present invention, a temperature regulating system for a plant tissue culture tank 200 is described. In the temperature control system, a temperature control water tank A3 is provided with a heat exchange structure A23, and in order to save resources, the heat exchange structure A23 is communicated with a supply unit A1, and hot water in a water containing cavity A301 can be heated or cooled by the supply unit A1.

Specifically, the temperature-adjusting water tank A3 has a heat exchanging structure a23 disposed against the water containing chamber a 301. The conveying unit A2 comprises a liquid inlet pipe A24 and a liquid outlet pipe A25 which are communicated with the supply unit A1 and the heat exchange structure A23 and are used for respectively leading in and leading out liquids with different temperatures to the heat exchange structure A23.

In this embodiment, by setting the heat exchange structure a23 in the temperature-adjusting water tank A3, the hot water in the temperature-adjusting water tank A3 is always kept at the set temperature when the supply unit A1 is running, so that the temperature-adjusting water tank A3 is ensured to be capable of immediately delivering hot water to the temperature-controlling layer when the supply unit A1 stops supplying or maintaining, the water of the temperature-adjusting water tank A3 does not need to wait for heating, and the standby heat exchange loop of the temperature-adjusting system is ensured to be capable of quickly taking over the main heat exchange loop to maintain the normal temperature of the plant tissue culture tank 200.

In an embodiment of the present invention, as shown in FIG. 1, a temperature regulating system for a plant tissue culture tank 200 is described.

The supply unit A1 comprises a hot water supply main pipe E200 and a hot water recovery main pipe E100 which are arranged in parallel, the conveying unit A2 comprises a hot water supply branch pipe E20 and a hot water recovery branch pipe E10, one end of the hot water supply branch pipe E20 is connected with the hot water supply main pipe E200, the other end is connected with the temperature control layer 31, one end of the hot water recovery branch pipe E10 is connected with the temperature control layer 31, and the other end is connected with the hot water supply main pipe E200.

The water inlet pipe A21 is connected with the hot water supply main pipe E200, the water outlet pipe A22 is connected with the hot water recovery main pipe E100, and the water containing cavity A301 and the temperature control layer 31 are connected in series in the same circuit.

In another embodiment, a water pump a211 is disposed in the water inlet pipe a21 in series, and is used for delivering the water in the water containing cavity a301 to the temperature control layer 31.

In this embodiment, the main pipes for supplying and recovering hot water are respectively provided in the supply unit A1, and the main pipes are connected into a complete circulation loop through the branch pipes corresponding to the main pipes, so that the supply unit A1 and the temperature-adjusting water tank A3 can exchange heat with the plant tissue culture tank 200, thereby providing stable growth conditions for the proliferation of plant in-vitro tissues such as adventitious roots. The water inlet pipe A21 of the temperature-adjusting water tank A3 is connected with the water pump A211 in series to provide power for a circulation loop formed by the temperature-adjusting water tank A3 and the temperature-controlling layer, so that hot water can be circulated independently.

Referring to fig. 1 and 3, in an embodiment of the present invention, a temperature regulating system for a plant tissue culture tank 200 is described.

In order to reduce the resistance of the hot water being transported between the supply unit A1 and the tempering tank A3 and to avoid that the water pump a211 blocks the hot water from flowing into the tempering tank A3, a branch pipe a212 connected in parallel with the water pump a211 is provided on the water inlet pipe a 21. Both ends of the branch pipe A212 are respectively connected with a water inlet end and a water outlet end of the water pump A211 communicated with the water inlet pipe A21, so that hot water flowing into the temperature-adjusting water tank A3 by the supply unit A1 bypasses the water pump A211 from the branch pipe A212.

In the present embodiment, by providing the branch pipe a212 in parallel to the water inlet pipe a21 provided with the water pump a211, the resistance of the supply unit A1 to the hot water supplied to the temperature-adjusting water tank A3 is reduced.

Referring to FIGS. 1 and 2, in an embodiment of the present invention, a temperature regulating system for a plant tissue culture tank 200 is described.

In order to reduce the temperature of the hot water in the tempering tank A3, the supply unit A1 comprises a chilled water supply main C200 and a cold drain pipe 300 arranged in parallel for delivering to the heat exchange structure a23 and for heat transfer with the hot water in the water containing chamber a 301.

In particular, the feed pipe a24 comprises a chilled water feed pipe a241. The drain pipe a25 includes a chilled water drain pipe a251. One end of the chilled water liquid inlet pipe A241 is connected with the chilled water supply main pipe C200, the other end is connected with the heat exchange structure A23, one end of the chilled water liquid outlet pipe A251 is connected with the heat exchange structure A23, and the other end is connected with the cold drain pipeline 300.

In this embodiment, the supply unit A1 is provided with the chilled water supply main pipe C200 and the cold drain pipe 300 and is connected with the heat exchange structure a23, so that the temperature adjusting system can quickly adjust the heat exchange between the heat exchange structure a23 and the temperature adjusting water tank A3, and ensure that the hot water in the temperature adjusting water tank A3 is always kept at the set temperature.

In order to raise the temperature of the hot water in the tempering tank A3, the supply unit A1 comprises a main steam supply pipe B100 and a heat drain pipe 400 arranged in parallel for delivering to the heat exchange structure a23 and for heat transfer with the hot water in the water containing chamber a 301.

In particular, the feed pipe a24 includes a steam feed pipe a242. The liquid outlet pipe A25 comprises a steam liquid outlet pipe A252. One end of the steam liquid inlet pipe A242 is connected with the steam supply main pipe B100, the other end is connected with the heat exchange structure A23, one end of the steam liquid outlet pipe A252 is connected with the heat exchange structure A23, and the other end is connected with the heat drain pipe 400.

In this embodiment, the steam supply main pipe B100 and the heat drain pipe 400 are disposed in the supply unit A1 and connected to the heat exchange structure a23, so that the temperature adjusting system can quickly adjust the heat exchange between the heat exchange structure a23 and the temperature adjusting water tank A3, and ensure that the hot water in the temperature adjusting water tank A3 is always kept at the set temperature.

In order to ensure the reliability and safety of controlling the steam liquid inlet pipe A242, two parallel branches are arranged on the steam liquid inlet pipe A242: one is connected with a manual valve 5 and a pneumatic valve 4 in series, and the other is connected with the manual valve 5 only in series, so as to manually control the on-off of the steam liquid inlet pipe A242 in the event of automatic control failure.

In another embodiment, a steam outlet pipe A252 is connected between the temperature-adjusting water tank A3 and the hot drain pipe 400. The steam liquid outlet pipe A252 is split into two pipelines at one end connected with the temperature-regulating water tank A3, wherein one pipeline is connected with a one-way valve and a pneumatic valve 4 in series, and the other pipeline is connected with the pneumatic valve 4 only in series.

In this embodiment, two parallel pipelines for controlling the on-off state are respectively arranged on the steam liquid inlet pipe a242 and the steam liquid outlet pipe a252, so that the steam circulation loop of the temperature regulating water tank A3 can not only realize automatic control through pneumatic operation, but also switch the on-off state of the steam circulation loop manually when the automatic control fails or is maintained, thereby avoiding the situation that the supply unit A1 cannot be used for heating in the temperature regulating water tank A3, and improving the stability and reliability of the temperature regulating system.

Example two

In the embodiment of the present invention, as shown in FIGS. 2 and 3, a piping structure of a continuous culture apparatus 3 is described. Wherein the cascade culture apparatus 3 comprises at least two plant tissue culture tanks 200 for culturing plant in-vitro tissues or cells. In particular, two plant tissue culture tanks 200 are connected in parallel, and air, nutrients, heat, cleaning, disinfection and the like are supplied from a supply source to each plant tissue culture tank independently through a pipeline structure.

Specifically, the pipeline structure includes: a main pipeline 1 and a branch pipeline 2. The main pipeline 1 and the cascade culture device 3 are arranged separately.

Wherein the main pipeline 1 is connected with a plurality of devices in parallel, and a plurality of main pipelines 1 extend along a straight line and are parallel to each other. Each tank is provided with a branch line 2 for exchanging or circulating substances with the main line 1. The branch pipeline 2 is connected between the main pipeline 1 and the continuous culture device 3 and communicates the main pipeline 1 with the continuous culture device 3.

In the embodiment, the pipeline structures connected to the culture device are optimally arranged according to the size of the interception area, wherein the interception area of the main pipeline 1 is large and is used for intensively supplying substances; the cut-off area of the branch pipe 2 is small for introducing the substance of the main pipe 1 into the culture apparatus. Different main pipelines 1 are arranged in parallel, so that a plurality of branch pipelines 2 can be connected in the extending direction of each main pipeline 1, and the pipelines can be rapidly identified during maintenance, thereby improving the regularity of the pipeline structure and reducing the ineffective occupation of the pipelines to the space.

In the embodiment of the present invention, as shown in FIG. 2, a piping structure of a continuous culture apparatus 3 is described.

Specifically, the continuous culture apparatus is provided with at least two plant tissue culture pots 200 of unequal volumes. Take a culture apparatus with two plant tissue culture tanks as an example. For convenience of distinction, a small-sized plant tissue culture tank is called a seed tank, and a large-sized plant tissue culture tank is called a fermenter.

In order to reduce the number of times of inoculating the cultured adventitious roots, adventitious buds, etc. to the culture apparatus and to achieve the purpose of repeatedly proliferating the adventitious roots, adventitious buds, etc., a plant tissue culture tank for culturing isolated tissues or cells of a plant is separately provided as a seed tank 201 and a fermentation tank 202.

The seed pot 201 is used for inoculating adventitious roots and adventitious buds, and is used as seeds for rapidly breeding the adventitious roots and the adventitious buds in the next period of the fermentation pot 202.

In particular, the volume of the seed tank 201 is smaller than the volume of the fermenter 202 by 1/3 to 1/5 of the volume of the fermenter 202.

The process of transferring and returning seeds in actual production is that after all the culture solution and adventitious roots in the seed tank 201 are led into the fermentation tank 202, the nutrient solution in the fermentation tank 202 is lifted to a set water level to dilute the concentration of the adventitious roots, and then the mixed liquid of the adventitious roots and the nutrient solution after the concentration is diluted is immediately reversely conveyed into the seed tank 201.

Specifically, the continuous culture device 3 is provided with at least one seed tank 201 and one fermentation tank 202, respectively, and the seed tank 201 and the fermentation tank 202 are connected in parallel to the main pipeline 1 through the branch pipeline 2. A seed pipe and a seed reversing pipe are connected between the seed tank 201 and the fermenter 202.

The seed pipe is used to introduce all of the adventitious root culture solution in the seed tank 201 into the fermentation tank 200. The reverse pipe is used to return the diluted mixed liquor in the fermenter 202 to the seed tank 201.

In this embodiment, by setting the continuous culture apparatus as the seed tank 201 and the fermentation tank 202, the number of times of inoculation to the culture apparatus is reduced, the purpose of multiple circulation proliferation of adventitious roots, adventitious buds and the like is achieved, and the efficiency of mass production of plant tissues is greatly improved.

In the embodiment of the present invention, as shown in FIGS. 2 and 3, a piping structure of a continuous culture apparatus 3 is described. The main lines 1 are provided with a plurality of main lines 1 which are arranged in parallel, and each main line 1 carries different media.

In particular, the main line 1 comprises a hot water supply main E200 and a hot water recovery main E100. Accordingly, the branch pipe 2 includes a hot water supply branch pipe E20 and a hot water recovery branch pipe E10.

The hot water supply branch pipe E20 has one end connected to the continuous culture apparatus 3 and the other end connected to the hot water supply main pipe E200 for supplying hot water to the continuous culture apparatus 3. One end of the hot water recovery branch pipe E10 is communicated with the continuous culture device 3, and the other end is communicated with the hot water recovery main pipe E100 for leading out the hot water in the continuous culture device 3.

In another embodiment, the main pipeline 1 comprises a chilled water supply main pipe C200 and a chilled water recovery main pipe C100. Correspondingly, the branch pipe 2 comprises a chilled water supply branch pipe C20 and a chilled water recovery branch pipe C10.

The chilled water supply branch pipe C20 has one end connected to the continuous culture apparatus 3 and the other end connected to the chilled water supply main pipe C200, and is used for supplying chilled water to the continuous culture apparatus 3. One end of the chilled water recovery branch pipe C10 is communicated with the continuous culture device 3, and the other end is communicated with the chilled water recovery main pipe C100 for guiding out chilled water in the continuous culture device 3.

In another embodiment, the main pipeline 1 includes a cooling water supply main pipe D200 and a cooling water recovery main pipe D100. Accordingly, the sub-piping 2 includes a cooling water supply sub-piping D20 and a cooling water recovery sub-piping D10.

The cooling water supply branch pipe D20 has one end connected to the continuous culture apparatus 3 and the other end connected to the cooling water supply main pipe D200, and is used for supplying cooling water to the continuous culture apparatus 3. One end of the cooling water recovery branch pipe D10 is communicated with the continuous culture device 3, and the other end is communicated with the cooling water recovery main pipe D100 for guiding out the cooling water in the continuous culture device 3.

In this embodiment, the pipeline structures are respectively provided with the main pipelines 1 for supplying and recovering the medium with different temperatures, and the pipeline structures are connected into a complete circulation loop through the branch pipelines 2 corresponding to the main pipelines 1, so that the pipeline structures can exchange heat with the culture device, thereby providing stable growth conditions for the proliferation of the isolated tissues of the plant bodies such as adventitious roots. And the parallel arrangement among different main pipelines 1 makes the pipeline structure regular, and is convenient for maintain.

In the embodiment of the present invention, as shown in FIGS. 2 and 3, a piping structure of a continuous culture apparatus 3 is described.

In order to individually control the material circulation of each plant tissue culture tank 200 in the continuous culture apparatus, pneumatic valves 4 for controlling the on-off of the pipelines are provided on the hot water supply branch pipe E20, the chilled water supply branch pipe C20 and the cooling water supply branch pipe D20.

In this embodiment, the pneumatic valve 4 is connected in series on the pipeline, so that the pipeline of the continuous culture device can realize automatic control of the on-off state, and the on-off control of the pipeline can be more convenient and rapid.

In order to improve the reliability of pipeline control, the pipeline can still change the on-off state when the pneumatic valve 4 fails or is maintained so as to maintain the continuous operation of the culture device. The hot water supply branch pipe E20, the chilled water supply branch pipe C20 and the cooling water supply branch pipe D20 are also provided with a manual valve 5 for controlling the on-off of a pipeline.

Specifically, the manual valve 5 is connected in series with the pneumatic valve 4.

In this embodiment, the manual valve 5 is connected in series to the pipeline, so that the pipeline of the continuous culture device can still change the on-off state when the pneumatic valve 4 fails or is maintained, so as to maintain the continuous work of the continuous culture device, and improve the reliability of the pipeline structure.

In the embodiment of the present invention, as shown in FIG. 1, a piping structure of a continuous culture apparatus 3 is described.

In order to reduce the number of connection ports formed in the culture apparatus, the hot water supply branch pipe E20, the chilled water supply branch pipe C20 and the cooling water supply branch pipe D20 are simultaneously connected to one input pipe 6 provided in the cascade culture apparatus 3.

In this embodiment, by arranging the input tube 6 on the culture device and connecting multiple branch tubes with the input tube 6 in parallel, the connection ports of the culture device are reduced, the difficulty in manufacturing the culture device can be reduced, and the tightness of the culture device can be improved.

In the embodiment of the present invention, as shown in FIGS. 1, 2 and 3, a piping structure of a continuous culture apparatus 3 is described.

In order to improve the safety and reliability of the pipeline structure, a loop capable of directly discharging high-heat medium is additionally arranged on the pipeline structure, so that the maintenance of the circulation loop is facilitated. The main pipe 1 is further provided with a heat drain pipe 400 having a pressure reducing filter 410. A discharge branch 420 for communicating with the pressure reducing filter 410 is provided between the input pipe 6 and the branch pipe 2, and the medium supplied in the main pipe 1 can be directly communicated with the heat drain pipe 400 without passing through the cascade culture device.

In another embodiment, in order to accurately form a circulation loop after sharing one input pipe 6 by the branch pipe 2, the hot water recovery branch pipe E10, the chilled water recovery branch pipe C10 and the cooling water recovery branch pipe D10 are provided with a pneumatic valve 4 and/or a manual valve 5 for controlling on-off of the pipes.

The pneumatic valves 4 and/or the manual valves 5 on the hot water recovery branch pipe E10, the chilled water recovery branch pipe C10 and the cooling water recovery branch pipe D10 are correspondingly matched with the pneumatic valves 4 and/or the manual valves 5 on the hot water supply branch pipe E20, the chilled water supply branch pipe C20 and the cooling water supply branch pipe D20, so that a complete circulation loop is formed.

Example III

In an embodiment of the present invention, as shown in fig. 1 and 2, a gas supply system for a plant tissue culture tank 200 is described. The air supply system includes an air supply main pipe a100 and an air intake branch a110 connected to the air supply main pipe a 100.

In order to better break down the air into small bubbles that dissolve in the culture medium, and increase the dissolution rate of the air in the culture medium, the air supply system further includes an air intake structure 40 provided on the sidewall of the plant tissue culture tank 200. One end of the air inlet structure 40 extends into the plant tissue culture tank 200, and the other end of the air inlet structure 40 extends outside the plant tissue culture tank 200 in a hanging manner and is connected with the air inlet branch A110 for sending the air in the air supply main pipe A100 into the plant tissue culture tank 200.

In this embodiment, the air supply main pipe a100 is connected to the air supply device and can provide air with stable pressure, the plurality of plant tissue culture tanks 200 can be connected to the air supply main pipe a100 in parallel via the air inlet branches, the air carried in the air supply main pipe a100 is respectively conveyed to each plant tissue culture tank 200 through the plurality of air inlet branches in parallel, and then the air is dispersed and a large number of fine bubbles are formed in the culture solution through the air inlet structure 40, so that the air content in the culture solution is improved, and the growth and proliferation of adventitious roots and the like are better promoted.

Referring to fig. 1, 2 and 3, in an embodiment of the present invention, a gas supply system for a plant tissue culture tank 200 is described.

In order to prevent the liquid in the plant tissue culture pot 200 from flowing into the air inlet branch A110, a check valve A120 is also arranged in the air supply system. A check valve a120 is disposed in series between the intake structure 40 and the intake branch a110.

Specifically, as shown in fig. 4, 5 and 6, the air intake structure 40 includes an air intake portion 41, an aeration portion 42, and a conduit 43 connecting the air intake portion 41 and the aeration portion 42. Wherein the conduit 43 is formed in a straight bar shape, and the air inlet portion 41 and the aeration portion 42 are connected to both ends of the conduit 43, respectively.

In particular, the center of the aeration portion 42 is provided with a cavity. The cavity communicates with the conduit 43 and the walls of the cavity are provided with micropores which communicate from the outside of the aeration section 42 into the cavity. A large number of micropores are densely and uniformly distributed throughout the cavity wall. The check valve a120 is located outside the tank wall 11 of the plant tissue culture tank 200 and is connected at one end to the air inlet 41 and at the other end to the air inlet branch a110.

In this embodiment, by adding the check valve a120 to the air inlet portion 41 of the air inlet structure 40, the air inlet branch a110 communicated with the inside of the plant tissue culture tank 200 can only unidirectional convey air to the plant tissue culture tank 200, thereby avoiding the flowing back of adventitious roots and culture fluid in the plant tissue culture tank 200 into the pipeline and improving the safety and reliability of the plant tissue culture tank 200 system.

Since the air contains a large amount of impurities, microorganisms and bacteria, a filtering device A130 is arranged in the air supply system to avoid that microorganisms and bacteria and the like are propagated in the culture solution and then consume nutrients in the culture solution, and even cause in-vitro cytopathy and putrefaction of the plant body.

Specifically, the filter device a130 is disposed in series in the intake branch a 110. Thus, bacteria and impurities are removed by the air passing through the filtering means A130, so that the air entering the plant tissue culture tank 200 is largely purified.

The filter element of the existing filter device A130 basically adopts polytetrafluoroethylene and other materials, and the filter element made of polytetrafluoroethylene is easy to break down by impurities and the like after being used for a period of time, so that the filter element loses the capability of filtering bacteria and impurities, and the service life of the filter element is short. In order to extend the service life of the filter cartridge and increase the period of maintenance of the filter device a130, the filter cartridge is provided to be made of silicate material.

In this embodiment, the filter element is made of silicate material by improving the filter element material, so that the strength of the filter element is improved by utilizing the characteristics of high hardness and high strength of silicate, the service life of the filter element is greatly prolonged, the period of maintaining the filter device A130 is prolonged, and the plant tissue culture tank 200 can work for a longer time.

In an embodiment of the present invention, as shown in FIG. 1, a gas supply system for a plant tissue culture tank 200 is described.

In order to conveniently disinfect the air supply system, the air supply system is also communicated with a steam supply main pipe B100.

Specifically, the main steam supply pipe B100 is provided with a filtering branch a140 connected to the intake branch a 110. The connection end of the filtering branch a140 and the air inlet branch a110 is arranged in front of the connection end of the inlet of the filtering device a130 and the air inlet branch a 110.

In this embodiment, the filtering branch circuit a140 is used to connect the filtering device a130 and the steam supply main pipe B100, so that the filtering device a130 can be sterilized by introducing high-temperature steam before the air supply system is started, thereby ensuring that bacteria, impurities and the like on the filtering device a130 are cleaned up and are in good working conditions, greatly improving the filtering effect of the filtering device a130, ensuring the cleanliness of air entering the plant tissue culture tank 200, and being beneficial to maintaining the good growth environment of the plant tissue culture tank 200.

In an embodiment of the present invention, as shown in FIG. 1, a gas supply system for a plant tissue culture tank 200 is described. In order to enable high-temperature steam to be directly introduced into the plant tissue culture tank 200, a tank extinction branch A150 communicated with the plant tissue culture tank 200 is further provided.

Specifically, one end of the tank eliminating branch line a150 is connected to the steam supply main pipe B100, and the other end of the tank eliminating branch line a150 is connected after the connection end of the outlet of the filtering device a130 and the air inlet branch line a 110. So that the steam supply main pipe B100 and the air supply main pipe a100 share one pipe connected to the plant tissue culture tank 200. The connecting ports arranged on the plant tissue culture tank 200 are reduced, so that the difficulty in manufacturing the culture device can be reduced, and the tightness of the culture device can be improved.

The air supply system is also provided with a backup branch a160 for manually switching the on-off state. The inlet end of the standby branch A160 is connected with the main air supply pipe A100, and the outlet end of the standby branch A160 is connected before the connection end of the filtering branch A140 and the air inlet branch A110. So that the backup branch a160 is connected to the main air supply pipe a100 in parallel with the intake branch a110 and is simultaneously communicated to the front of the filter device a 130.

In this embodiment, the air of the air supply main pipe a100 can be conveyed to the plant tissue culture tank 200 through the air inlet branch a110 and can be conveyed to the plant tissue culture tank 200 through the standby branch a160, so that a standby control loop is added for the air supply system, the air content of the nutrient solution in the plant tissue culture tank 200 can be ensured to be normally maintained and regulated when the air inlet branch a110 is damaged or maintained, and the growth condition of adventitious roots is ensured to be stable and controllable.

Referring to FIGS. 1 and 2, in one embodiment of the present invention, a gas supply system for a plant tissue culture tank 200 is described.

To increase the air content of the culture solution in the plant tissue culture tank 200, the air supply system is added to the connection end for supplying air to the plant tissue culture tank 200, and at least two air inlet structures 40 are symmetrically distributed at the tank bottom of the plant tissue culture tank 200.

Specifically, the multiple air inlet structures 40 are connected to the same air inlet branch a110 and are connected to the tank eliminating branch a150 and behind the connection end of the air inlet branch a110, so that air in the air inlet branch a110 can be synchronously introduced into the plant tissue culture tank 200 through the multiple air inlet structures 40, and the contact surface between the air and the culture solution is greatly improved.

In this embodiment, the air supply system is communicated with the plant tissue culture tank 200 through the plurality of air inlet structures 40, so that air can enter the plant tissue culture tank 200 from a plurality of positions, the contact surface between the air and the culture solution is increased, the dissolution rate of the air is effectively improved, and the air is fully diffused into the culture solution from the periphery of the plant tissue culture tank 200.

To keep the air pressure in plant tissue culture tank 200 stable, the air supply system also includes an air exhaust line A170. One end of the exhaust pipeline A170 is connected with the top of the plant tissue culture tank 200, and the other end of the exhaust pipeline A170 is communicated with the outside, so that the exhaust gas in the plant tissue culture tank 200 can be directly discharged into the atmosphere.

In another embodiment, a gas filter A180 is also disposed in series in the exhaust line A170 for trapping moisture and nutrients contained in the gas in the plant tissue culture tank 200 within the plant tissue culture tank 200.

In order to ensure that the gas filter a180 is in a low temperature state for a long time to improve the effect of filtering moisture, nutrients and nutrients in the exhaust gas, the gas filter a180 is provided with a cooling structure for coating the filter element, so as to cool the gas filter a 180.

In particular, the cooling structure is provided with a pipeline for leading in and out liquid. The pipeline can be filled with low-temperature cooling water or low-temperature chilled water. The difference between chilled water and cooling water is that chilled water is 10-20 degrees warmer than cooling water.

In this embodiment, the top of the plant tissue culture tank 200 is provided with the exhaust pipeline a170, so that the air flowing into and out of the plant tissue culture tank 200 is controlled by the exhaust pipeline a170 and the air inlet structure 40, the air pressure value in the plant tissue culture tank 200 can be controlled to be stable by using the air supply system, and the air inlet and outlet speeds can be correspondingly adjusted according to the optimal growth air pressure of plant tissues, so that the adventitious roots can be ensured to obtain sufficient air. Meanwhile, the serial gas filter A180 in the exhaust pipeline A170 can prevent external impurities from entering the plant tissue culture tank 200 through the exhaust pipeline A170 and prevent water and nutrients in the plant tissue culture tank 200 from diffusing into the outside.

Example IV

In an embodiment of the present invention, as shown in FIG. 5, an air intake structure 40 is provided on a plant tissue culture tank 200. The air intake structure 40 includes an air intake portion 41, an aeration portion 42, and a conduit 43 connecting the air intake portion 41 and the aeration portion 42. Wherein the conduit 43 is formed in a straight bar shape, and the air inlet portion 41 and the aeration portion 42 are connected to both ends of the conduit 43, respectively.

In particular, the center of the aeration portion 42 is provided with a cavity. The cavity communicates with the conduit 43 and the walls of the cavity are provided with micropores which communicate from the outside of the aeration section 42 into the cavity. A large number of micropores are densely and uniformly distributed throughout the cavity wall.

Preferably, the aeration portion 42 is made of a titanium alloy material such that adventitious roots cannot adhere to the outer surface of the aeration portion 42, thereby avoiding adventitious root accumulation spoilage.

In this embodiment, a large number of micropores are densely formed on the side wall of the aeration portion 42, and all the micropores are communicated with a cavity formed in the center of the aeration portion 42, so that gas entering the cavity of the aeration portion 42 through the gas guide pipe can be sprayed into the solution from all the micropores at the same time, thereby forming a large number of small bubbles in the solution at the same time, the small bubbles move outwards from the outer surface of the aeration portion 42 and float upwards, so that the small bubbles are prevented from being polymerized into large bubbles, the contact and dissolution rate of the gas and the solution are improved, and the circulating flow of the solution is promoted by the floating movement of a large number of small bubbles in the solution in a dispersing manner, and the dissolved oxygen effect is further improved.

In another embodiment of the present invention, as shown in fig. 6, an aeration portion 42 of an air intake structure 40 is described. In order to reduce the time for opening the micropores in the aeration portion 42 and to ensure a more uniform distribution of micropores in the aeration portion 42, the air can be decomposed into small bubbles dissolved in the culture medium. The aeration portion 42 is formed by stacking a large number of small particles of a metal material having a diameter of less than 1mm and sintering them together to form a housing having a cavity in the center.

Specifically, the small particles are set to spherical shapes. A large number of small particles are closely adhered and there are gaps between adjacent small particles. The gaps are arranged in sequence and connected in the radial direction of the aeration portion 42 to form micropores extending from the inner surface of the chamber wall to the outer surface of the chamber wall. Thus, the outer surface of the aeration portion 42 is covered with micropores, similar to the meshes of a screen.

Preferably, the small particles are made of a metallic titanium alloy, so that the adhesion of adventitious roots to the outer surface of the aeration portion 42 can be avoided.

In this embodiment, the aeration portion 42 is provided by a method of sintering and adhering small particles, so that the distribution of micropores formed on the aeration portion 42 is more uniform, and therefore, air can be decomposed into small bubbles dissolved in the culture solution better, which is beneficial to increasing the dissolution rate of air in the culture solution.

In another embodiment of the present invention, as shown in FIG. 6, an air intake structure 40 is described that can be more conveniently mounted to a plant tissue culture tank.

Specifically, the aeration portion 42 provided with the air intake mechanism has a long cylindrical shape, and one end of the aeration portion 42 is connected to the conduit 43. The central axis of the aeration portion 42 is arranged to coincide with the central axis of the tip of the conduit 43 and to extend in a straight line in a direction away from the conduit 43. Meanwhile, the conduit 43 is long and straight, so that the aeration portion 42 and the conduit 43 are arranged to coincide with the central axis.

In this embodiment, the aeration portion 42 is formed in a long cylindrical shape and extends outward from the end of the conduit 43, so that the diameter of the opening in the plant tissue culture tank can be reduced, and the contact surface between the air inlet structure 40 and the culture medium can be made larger, which is advantageous for sufficiently dispersing and dissolving air in the culture medium.

In another embodiment of the present invention, as shown in fig. 6, an air intake structure 40 is described that can be more conveniently secured. A seat plate 44 provided on the air intake structure 40 is located in the middle of the air intake portion 41 and the aeration portion 42, separating the air intake portion 41 and the aeration portion 42.

Specifically, a seat plate 44 is attached to the duct 43 and extends radially outwardly of the duct 43, forming a shape in which the duct 43 passes vertically through the center of the seat plate 44. In particular, the seat plate 44 is located between both ends of the duct 43, the aeration portions 42 are distributed on one side of the seat plate 44, and the air intake portion 41 is disposed on the other side of the seat plate 44.

Preferably, the seat plate 44 is provided in a circular flat plate shape so that the air intake structure 40 can be rotated around the duct 43 when installed.

In this embodiment, the seat board 44 is disposed on the air inlet structure 40, so that the air inlet structure 40 and the plant tissue culture tank are more convenient to install and connect, and the air inlet structure 40 can rotate around the conduit 43, so that the difficulty of assembly work is reduced.

In another embodiment of the present invention, as shown in fig. 6, an intake structure 40 with improved intake performance is described. The conduit 43 of the air intake structure 40 is optimally adjusted for better dissolution of the air into the culture medium.

Specifically, the setting conduit 43 includes an elongated straight intake section 431. The air inlet sections 431 are respectively suspended to at least one length toward both sides of the seat plate 44, thereby facilitating the better connection and fixation of the aeration section 42 and the air inlet section 41 to the guide duct 43.

The aeration portion 42 and the air inlet portion 41 are respectively connected with the ends of the air inlet section 431 which extend out of the side surface of the seat plate 44, so that the aeration portion 42 and the air inlet portion 41 are separated on two sides of the seat plate 44.

In another embodiment of the present invention, in order to increase the position of the aeration section 42 in the plant tissue culture tank after being mounted to the plant tissue culture tank, the guide tube 43 is further provided with an air outlet section 432 disposed at an angle to the air inlet section 431.

An air outlet section 432 has one end connected to the air inlet section 431 and the other end connected to the aeration section 42. In particular, the angle between the outlet section 432 and the inlet section 431 is set to be greater than 90 °.

In another embodiment, the conduit 43 is further provided with a transition 433 connected between the inlet section 431 and the outlet section 432 for further widening the range of positions of the aeration section 42 in the plant tissue culture tank in cooperation with the seat plate 44.

In particular, the transition 433 is arranged parallel to the seat plate 44. One end of the transition section 433 is connected to the air inlet section 431, and the other end is connected to the air outlet section 432. The air outlet section 432 is disposed obliquely to the transition section 433 and extends from the transition section 433 in a direction away from the seat plate 44.

In this embodiment, the optimizing conduit 43 includes a plurality of straight sections and the adjacent straight sections are connected in a bending manner, so that when the air inlet structure 40 is installed in the plant tissue culture tank, the air inlet structure 40 is rotated to ensure that the aeration portion 42 can be positioned at more positions of the plant tissue culture tank, so that the hoverable position range of the aeration portion 42 in the plant tissue culture tank is enlarged, and bubbles can be fully diffused into the culture fluid from the periphery of the aeration portion 42.

In another embodiment of the present invention, as shown in fig. 5 and 6, an intake structure 40 capable of rapid connection is introduced. The air intake portion 41 of the air intake structure 40 includes a quick disconnect 45.

Specifically, a medium speed disconnect 45 is provided at the end of the conduit 43 in the air intake portion 41. The quick release connector 45 is conical in shape. The conical bottom surface of the quick release connector 45 is superposed on the air inlet end surface of the duct 43. The inlet of the duct 43 is located in the center of the bottom surface. The tapered surface of the quick release connector 45 is directed toward the seat plate 44 and gradually contracted to the outer peripheral surface of the guide tube 43.

In another embodiment of the present invention, as shown in fig. 6, a quick disconnect 45 having a sealed air intake structure 40 is described.

The conical bottom surface of the quick disconnect 45 is provided with a seal groove 451. The seal groove 451 is recessed from the bottom surface toward the inside of the quick release connector 45 and forms a recess on the bottom surface. In particular, a seal groove 451 surrounds the outer periphery of the inlet of said duct 43.

As shown in FIG. 4, the present invention also provides a plant tissue culture tank 200 having any of the above-described air intake structures 40. The bottom of plant tissue culture pot 200 is provided in an inverted conical shape. The conical side wall is provided with a connection 51 for connecting the air inlet structure 40. The air inlet structure 40 is connected with the interface 51, and the aeration part 42 is positioned in the cavity of the plant tissue culture tank 200, and the air inlet part 41 is overhanging outside the plant tissue culture tank 200.

In this embodiment, the plant tissue culture tank 200 is provided with the air inlet structure 40, so that a large number of small bubbles can be formed in the solution by air when adventitious roots are cultivated, the small bubbles move outwards and upwards from the aeration portion 42 to promote the circulation flow of the nutrient solution, and the large number of small bubbles are dispersed in the solution to further improve the contact and dissolution rate of the air and the nutrient solution, provide stable conditions for the proliferation of the adventitious roots, and facilitate the improvement of the cultivation efficiency and quality of the adventitious roots.

In another embodiment of the present invention, as shown in FIG. 4, a mounting assembly of an air intake structure capable of rotating at an arbitrary angle around its central axis when connected to a culture tank is introduced. The mounting assembly includes a stack 52 having an annular shape.

When the air inlet structure 40 is fixedly installed, the stacking piece 52 and the interface 51 on the culture tank are connected through end surfaces and the seat plate 44 is clamped between the stacking piece 52 and the interface 51, so that the seat plate 44 can be rotated when the stacking piece 52 and the interface 51 are not tightly attached, and the position and the posture of the air inlet structure 40 relative to the culture tank can be adjusted.

Specifically, the lamination 52 is provided as an annular structure having an annular hole in the center. The stacking piece 52 is sleeved with the seat plate 44 and abuts with the edge of the annular hole of the stacking piece against the plate surface of the seat plate 44 facing the outer side of the plant tissue culture tank, so as to clamp the air inlet structure 40 on the plant tissue culture tank.

In another embodiment, the mounting assembly further includes a hub sleeve 53 having a cylindrical outer peripheral surface. The hub sleeve 53 is nested in the interface 51 and connected to the interface 51 with its outer peripheral surface. In particular, the axis of the hub kit 53 is perpendicular to the surface of the tank wall 11 of the culture tank and projects inward and outward of the tank wall 11 in the axial direction of itself.

Meanwhile, an inner circumferential surface of the hub unit 53, in which a center hole is formed, is coupled to the seat plate 44.

Further, the end of the hub sleeve 53 facing into the tank wall 11 is provided with a support ring 531. The support ring 531 extends from the inner circumferential surface of the hub kit 53 in the radial direction toward the central axis of the hub kit 53, and forms a through hole 521 that can pass through the aeration portion 42.

The seat plate 44 is fitted to the inner peripheral surface of the hub sleeve 53 from the outside of the tank wall 11, and the seat plate 44 abuts against the support ring 531.

In another embodiment, a more leak-tight mounting assembly is described. The mounting assembly includes a seal 54 for filling the gap between the contact surfaces.

Specifically, the seal ring 54 is made of rubber material, and has elasticity and good ductility. The seal ring 54 is provided between the seat plate 44 and the support ring 531, and is sandwiched between an end surface of the seat plate 44 facing the inside of the can and an end surface of the support ring 531.

In particular, when the seat plate 44 and the support ring 531 sandwich the seal ring 54, the thickness of the seal ring 54 is reduced, so that the projections and recesses on the end surface of the seat plate 44, which is close to the support ring 531, are filled with the seal ring 54, and the tightness of the installation assembly of the air intake structure 40 is improved.

Further, a support ring 531 is provided at the end of the hub kit 53 that is located within the tank wall 11. The through hole 521 of the support ring 531 is arranged coincident with the central axis of the central hole of the hub unit 53. And, one end face of the support ring 531 is flush with the end face of the hub unit 53 and the other is located in the central bore of the hub unit 53.

In particular, the support ring 531 is provided with a recess 532 in the end surface of the hub sleeve 53 that is located in the central bore. The opening of the recess 532 is directed towards the outside of the tank wall 11. The seal ring 54 is embedded in the groove 532 and protrudes from the end surface of the support ring 531. When the fixed intake structure 40 is installed, the seat plate 44 presses the seal ring 54, so that the seal ring 54 deforms to fill the groove 532.

In this embodiment, the seat plate 44 of the mounting assembly is in a sleeved fit with the hub sleeve 53 in a gap, the seat plate 44 is sleeved in the central hole of the hub sleeve 53 to flexibly rotate, and the seat plate 44 is clamped and fixed in the hub sleeve 53 by pressing the lamination piece 52 from the other side of the seat plate 44.

Specifically, the end surface of the hub member 53 protruding outside the tank wall 11 is provided with a connection hole 533. The connection hole 533 extends along the axial direction of the hub sleeve 53 for fixing the lamination member 52.

The lamination 52 is connected to the hub sleeve 53 at an end face, and sandwiches the seat plate 44. And the lamination member 52 is provided with a through hole 521 corresponding to the connection hole 533 for passing through the fixing screw.

The foregoing description is only illustrative of the preferred embodiment of the present invention, and is not to be construed as limiting the invention, but is to be construed as limiting the invention to any simple modification, equivalent variation and variation of the above embodiments according to the technical matter of the present invention without departing from the scope of the invention.

Claims

1. The temperature regulating system of the plant tissue culture tank comprises a supply unit (A1), a temperature control layer (31) coated on the outer surface of the plant tissue culture tank and a conveying unit (A2) communicated with the supply unit (A1) and the temperature control layer (31), and is characterized in that,

the device also comprises a temperature-regulating water tank (A3) communicated with the supply unit (A1), wherein the supply unit (A1) carries a plurality of liquids with different temperatures and leads in and out the temperature-regulating layer (31) and/or the temperature-regulating water tank (A3) through the conveying unit (A2);

the temperature-regulating water tank (A3) is provided with a water containing cavity (A301), and the water containing cavity (A301) and the temperature-controlling layer (31) are connected with the supply unit (A1) through the conveying unit (A2) to form a circulation loop; the conveying unit (A2) comprises a water inlet pipe (A21) and a water outlet pipe (A22) which are communicated with the supply unit (A1) and the water containing cavity (A301);

the hot water supply main pipe (E200) and the hot water recovery main pipe (E100) are arranged in parallel, the conveying unit (A2) comprises a hot water supply branch pipe (E20) and a hot water recovery branch pipe (E10), one end of the hot water supply branch pipe (E20) is connected with the hot water supply main pipe (E200), the other end of the hot water supply branch pipe is connected with the temperature control layer (31), one end of the hot water recovery branch pipe (E10) is connected with the temperature control layer (31), and the other end of the hot water recovery branch pipe is connected with the hot water recovery main pipe (E100);

the water inlet pipe (A21) is connected with the hot water supply main pipe (E200), the water outlet pipe (A22) is connected with the hot water recovery main pipe (E100), and the water containing cavity (A301) and the temperature control layer (31) are connected in series in the same loop;

The supply unit (A1) comprises a chilled water supply main pipe (C200) and a steam supply main pipe (B100), and the conveying unit (A2) comprises a liquid inlet pipe (A24) and a liquid outlet pipe (A25) which are communicated with the temperature-regulating water tank (A3) and the supply unit (A1) and provided with a plurality of branches;

the temperature regulating system also comprises a heat exchange structure (A23) and a master control unit electrically connected with the pneumatic valves connected in series in each branch of the liquid inlet pipe (A24); the heat exchange structure (A23) is arranged close to the water containing cavity (A301), and the main control unit is used for controlling the proportion of the liquid inlet pipe (A24) for conveying chilled water and high-temperature steam to the temperature-adjusting water tank (A3) and the heat exchange structure (A23);

the conical tank bottom of the plant tissue culture tank (200) is provided with an air inlet structure (40), and the air inlet structure (40) can be communicated with a steam supply main pipe (B100);

the air inlet structure (40) comprises an aeration part (42) positioned in the plant tissue culture tank (200), an air inlet part (41) overhanging the outer side of the tank wall and a conduit (43) supporting the aeration part (42) to be spaced from the tank wall;

the conduit (43) comprises an inlet section (431) perpendicular to the tank wall, a transition section (433) parallel to the tank wall, and an outlet section (432) arranged at an obtuse angle to the transition section (433); the air inlet structure (40) is provided with a circular flat plate-shaped seat plate (44), and the seat plate (44) is arranged at an interface (51) of the plant tissue culture tank (200); the air inlet section (431) vertically passes through the center of the seat board (44) and respectively extends at least a length to two sides of the seat board (44);

The aeration part (42) extends along a straight line from the end of the air outlet section (432) to form a long column, the aeration part (42) is provided with a cavity (421) communicated with the guide pipe (43), the cavity wall of the cavity is provided with a plurality of densely distributed micropores communicated with the inside and the outside of the cavity (421), the micropores are formed by stacking a large number of small particles with diameters smaller than 1mm, and gaps among different small particles extend from the inner surface of the cavity wall to the outer surface of the cavity wall.

2. A plant tissue culture tank tempering system according to claim 1, wherein a water pump (a 211) is arranged in series in said water inlet pipe (a 21) for delivering water in said water containing chamber (a 301) to said temperature controlling layer (31).

3. The plant tissue culture tank temperature regulating system according to claim 2, wherein a branch pipe (a 212) connected in parallel with the water pump (a 211) is arranged on the water inlet pipe (a 21), and two ends of the branch pipe (a 212) are respectively connected with a water inlet end and a water outlet end of the water pump (a 211) communicated with the water inlet pipe (a 21).

4. A plant tissue culture tank tempering system according to claim 1, wherein said supply unit (A1) comprises a cold drain pipe (300) arranged in parallel with said chilled water supply main (C200),

The liquid inlet pipe (A24) comprises a chilled water liquid inlet pipe (A241), the liquid outlet pipe (A25) comprises a chilled water liquid outlet pipe (A251), one end of the chilled water liquid inlet pipe (A241) is connected with the chilled water supply main pipe (C200), the other end of the chilled water liquid inlet pipe is connected with the heat exchange structure (A23), one end of the chilled water liquid outlet pipe (A251) is connected with the heat exchange structure (A23), and the other end of the chilled water liquid outlet pipe is connected with the cold drain pipeline (300).

5. A plant tissue culture tank tempering system according to claim 4, wherein said supply unit (A1) comprises a heat drain pipe (400) arranged in parallel with said steam supply main pipe (B100),

the liquid inlet pipe (A24) comprises a steam liquid inlet pipe (A242), the liquid outlet pipe (A25) comprises a steam liquid outlet pipe (A252), one end of the steam liquid inlet pipe (A242) is connected with the steam supply main pipe (B100), the other end of the steam liquid inlet pipe is connected with the heat exchange structure (A23), one end of the steam liquid outlet pipe (A252) is connected with the heat exchange structure (A23), and the other end of the steam liquid outlet pipe is connected with the heat exhaust pipeline (400).

6. The plant tissue culture tank temperature regulating system according to claim 5, wherein the steam liquid inlet pipe (A242) is provided with two parallel branches, one branch is connected with a manual valve (5) and a pneumatic valve (4) in series, and the other branch is connected with the manual valve (5) only in series, so that the on-off of the steam liquid inlet pipe (A242) is manually controlled when automatic control fails.

7. A plant tissue culture tank tempering system according to claim 6, wherein said vapour outlet pipe (a 252) splits into two pipes at the end connected to said tempering tank (A3), one in series with a non-return valve and a pneumatic valve (4), the other in series with a pneumatic valve (4) only.