CN108998372B

CN108998372B - Microorganism immobilized sphere preparation device

Info

Publication number: CN108998372B
Application number: CN201811156048.6A
Authority: CN
Inventors: 李祥锴; 裴亚欣; 陶晨
Original assignee: Lanzhou University
Current assignee: Lanzhou University
Priority date: 2018-09-30
Filing date: 2018-09-30
Publication date: 2023-04-11
Anticipated expiration: 2038-09-30
Also published as: CN108998372A

Abstract

The invention relates to a device for preparing microorganism immobilized spheres, which belongs to the technical field of microorganisms; a microorganism suspension storage tank and a gel liquid storage tank are arranged on the right side of the top of the cabin shell in parallel; a plurality of drip pipes are erected at the top of the cabin shell, a top cover is hinged to an opening at the upper end of each drip pipe, and a first connector, a second connector and a third connector are sequentially connected to the top cover in a through mode from right to left; the liquid outlet of the microbial suspension storage tank and the liquid outlet of the gel liquid storage tank are both connected with peristaltic pumps, the inlet ends of the peristaltic pumps are both connected with liquid guide pipes, and the liquid guide pipes are respectively arranged in the microbial suspension storage tank and the gel liquid storage tank. The residue on the inner wall of the container in the process of preparing the gel cell mixed solution in the transfer container is avoided; preventing the gel cell mixed liquid from being polluted by gas source impurities; the immobilization time is shortened; the preparation is continuously carried out, the labor is saved, and the practicability is stronger.

Description

Microorganism immobilized sphere preparation device

Technical Field

The invention relates to the technical field of microorganisms, in particular to a device for preparing a microorganism immobilized sphere.

Background

In industrial wastewater treatment applications, microbial cell immobilization technology is a very promising technology. By combining immobilization with dominant bacteria capable of degrading specific substances, the loss of efficient degrading bacteria can be effectively reduced, the biological toxicity of toxic wastewater to microorganisms is reduced, and the degradation efficiency of pollutants is improved. The common immobilization methods at present include adsorption, embedding, covalent bonding, and crosslinking. The embedding method has better comprehensive performance and higher survival rate of cells, and the embedding body is flexibly applied in reaction engineering, thereby becoming the most widely applied immobilization method in the whole immobilized microorganism technology. The embedding method is to wrap cells in a network structure of gel or in a semi-permeable polymeric film by a physical method, so that small molecular substrates and products can freely come in and go out, and microorganisms cannot leak out. Polyvinyl alcohol (PVA) has good hydrophilicity, chemical stability and mechanical property, and has strong antimicrobial decomposition capability, so that PVA is one of the immobilized materials widely applied in the sewage treatment embedding method in recent years. The irreversible plasticization of the polymer by crosslinking with boric acid is the most common method for preparing the carrier. However, the immobilized spheres made of the material usually have the problems of high PVA viscosity, easy coagulation, dripping gel cooling at 4 ℃ and the like.

The immobilized cells can be prepared in various shapes, such as granules, blocks, strips, films, or irregular shapes, depending on the application and the preparation method. At present, most of immobilized cells are prepared into granular beads, because the immobilized cells with irregular shapes are easy to wear, are easy to deform under pressure in a reactor, particularly a column reactor, and have poor flow rate, and the defects can be overcome by adopting a spherical shape. In addition, the circular beads have relatively high production efficiency due to the large surface area and the large contact area with the substrate. The catalytic activity of the immobilized cells is mainly concentrated on the surface layer of the gel, so the size of gel particles or gel blocks inevitably influences the reaction rate of the immobilized cells, and the optimal size of the gel particles prepared by the embedding method is considered to be 2-3mm, so that the efficient and rapid preparation of immobilized spheres with uniform size is very important in the pollutant treatment process.

The dripping method is a simple and common method for preparing the spherical immobilized microorganism in a laboratory. There are two types of methods for preparing pellets by the dropping method commonly used at present: the first is peristaltic pump method, which is a composite pump capable of pumping liquid and adding liquid and pressurizing in fixed time and fixed quantity. The pump rolls the silicon rubber tube by utilizing a plurality of driven wheels of the pump head, thereby extruding the gel cell mixed liquid in the silicon rubber tube out of the tube opening intermittently and constantly, and dripping the gel cell mixed liquid into a container filled with a forming agent to form uniform spheres. Although the method has convenient speed regulation, high speed and easy control of the size of the sphere, and can be used for manufacturing a large batch of spherical carriers, the residual quantity of the pipe wall is large due to high viscosity of the mixed liquid, and a plurality of peristaltic pumps and dropping liquid pipes are required to be configured and correspond one to one aiming at the situation that the peristaltic pumps and the dropping liquid pipes can not be shared during different liquid storage, or the mixed liquid is mixed and polluted; the second type is an air pressure method, which is to pour the gel cell mixed solution into a sealable container, pressurize the air, drip the mixed solution from a small opening at the other end, and drip the mixed solution into a forming agent to obtain beads, but the single air source cannot be effectively utilized in the dripping process, and the method is only suitable for preparing a small amount of single immobilized spheres. In addition, the method relates to compressed air but has no air source filtering device, the sample is easily polluted by air source impurities, and meanwhile, the preparation process of the mixed liquid needs to be transferred from an open container to a closed container, and because of high viscosity, more gel residues exist in the container.

Meanwhile, when the gel cell mixed solution is actually prepared, the gel solution is usually added into the microbial cell suspension under the condition that the temperature is higher than the room temperature but lower than the intolerance temperature of the microbes, the prepared mixed solution is not easy to coagulate, has low viscosity and is easy to drip, and the viscosity of the gel cell mixed solution is increased along with the reduction of the temperature in the subsequent dripping process, so that the dripping speed is influenced. Both of the above methods fail to effectively solve this problem. Additionally, drop-formed spheres typically have to be transferred to 4 ℃ and left overnight for better solidification, which is time and labor intensive.

Disclosure of Invention

Aiming at the defects and shortcomings of the prior art, the invention provides the microorganism immobilized sphere preparation device which is simple in structure, reasonable in design and convenient to use, and avoids the residue on the inner wall of the container in the process of preparing the gel cell mixed liquid in the transfer container; preventing the mixed liquid of the gel cells from being polluted by gas source impurities; the immobilization time is shortened; the preparation is continuously carried out, the labor is saved, and the practicability is stronger.

In order to achieve the purpose, the invention adopts the technical scheme that: the device comprises a cabin shell, a dripping nozzle, an air conduit, an air flow meter, an air filter, a condenser, an air pump, a magnetic stirrer, a first collector, a compressor, a power adapter, a controller, a liquid storage tank heat-insulating layer, a liquid conduit, a peristaltic pump, a microorganism suspension conduit, a gel liquid conduit, a mixer, an air valve, a dripping liquid pipe heat-insulating layer, a liquid level sensor, a microorganism suspension storage tank, a gel liquid storage tank, a dripping liquid pipe, a top cover, a silicon rubber heating sheet, a first temperature sensor and a second temperature sensor; the right side of the top of the cabin shell is provided with a microorganism suspension storage tank and a gel liquid storage tank in parallel; a plurality of drip pipes are erected at the top of the cabin shell, a top cover is hinged to an opening at the upper end of each drip pipe, and a first connector, a second connector and a third connector are sequentially connected to the top cover in a through mode from right to left; a liquid outlet of the microbial suspension storage tank and a liquid outlet of the gel liquid storage tank are both connected with peristaltic pumps, inlet ends of the peristaltic pumps are both connected with liquid guide pipes, the liquid guide pipes are respectively arranged in the microbial suspension storage tank and the gel liquid storage tank, and an outlet end of the peristaltic pump at the top of the microbial suspension storage tank is connected with the third interface through the microbial suspension guide pipe; the outlet end of the peristaltic pump at the top of the gel liquid storage tank is connected with the first interface through a gel liquid conduit; the electric control end of the blending device is movably arranged on the top cover through a bracket, and the stirring end of the blending device is arranged in the dropping liquid pipe after passing through the second connector; a dropping liquid pipe heat-insulating layer is wrapped outside the dropping liquid pipe, a liquid level detection area is arranged between the bottom end of the dropping liquid pipe heat-insulating layer and the outlet edge of the inverted cone-shaped opening at the bottom of the dropping liquid pipe, and a liquid level sensor is arranged outside the liquid level detection area; the inverted cone-shaped opening is connected with a drip nozzle, the drip nozzles penetrate through the top cover and are arranged inside the cabin shell, and each drip nozzle is correspondingly arranged at an inlet at the upper end of a first collector inside the cabin shell; the outer wall of the gel liquid storage tank is wrapped with a liquid storage tank heat-insulating layer; a silicon rubber heating sheet and a second temperature sensor are embedded in the liquid storage tank heat insulation layer and the dropping liquid pipe heat insulation layer; a condenser is arranged in the outer shell of the cabin body, and the first collector is arranged in the condenser; a first temperature sensor is fixed on the inner wall of the outer shell of the cabin body; the compressor and the power adapter are arranged inside the cabin shell from bottom to top and are positioned on the right side outside the condenser; the controller is embedded on the front wall of the cabin shell; the air pump and the air filter are arranged inside the cabin shell from bottom to top and are positioned on the left side outside the condenser, the outlet end of the air pump is connected with the air filter, the air filter is connected with the air flow meter, the air flow meter is embedded on the front wall of the cabin shell, the air flow meter is connected with an air guide pipe, the air outlet end of the air guide pipe is connected with a plurality of air valves through a plurality of branch pipes, and the air outlet end of each air valve is communicated with the third connector through a pipeline; the bottom parts of the gel liquid storage tank and the first collector are respectively provided with a magnetic stirrer, and the power adapter is electrically connected with the condenser, the compressor, the controller, the first temperature sensor, the second temperature sensor, the silicon rubber heating sheet, the liquid level sensor, the peristaltic pump, the magnetic stirrer and the air pump; the first temperature sensor, the second temperature sensor 33 and the liquid level sensor are all connected with a controller, and the controller is connected with a compressor, a magnetic stirrer, a blending device, a silicon rubber heating sheet, a peristaltic pump and an air pump.

Furthermore, the first collector is replaced by a second collector, a support is arranged in the cabin shell, the second collectors are arranged on the upper side and the lower side of the transverse plate of the support, the transverse plate of the support is in split connection with the longitudinal plate of the support, and the second collectors can be placed into the support to facilitate pre-cooling of coagulants in the collectors in advance.

Furthermore, a buckle is screwed on one side, far away from the hinged end, of the top cover, and the movable clamp is arranged in a clamping groove in the side wall of the dropping liquid pipe.

Furthermore, a cabin door is hinged at the front opening of the cabin shell.

The working principle of the invention is as follows: the power adapter supplies power to the power consumption elements including the controller; the controller controls the compressor of the heat exchanger through the first temperature sensor, and enables the interior of the outer shell of the cabin body to maintain stable low temperature through the condenser;

in order to reduce the residue of the gel liquid and the gel cell mixture on the corresponding pipelines, all parts in contact with the gel liquid and the gel cell mixture are made of hydrophobic materials. After the dropping nozzles are plugged, gel liquid is directly prepared in dropping liquid pipes and gel liquid storage tanks which are separated from respective heat insulation layers and top covers, the gel liquid is cooled to a certain temperature after being heated and dissolved, the gel liquid is inserted into respective heat insulation layers, microorganism suspension liquid is added, the top covers are covered and the buckles are locked, and after uniform mixing, subsequent dropping liquid is carried out, so that gel residue during secondary transfer is avoided;

in order to prevent the coagulation of gel cell mixed liquid in the dropping liquid pipe and gel liquid in the gel liquid storage tank, a dropping liquid pipe heat-insulating layer and a liquid storage tank heat-insulating layer are covered on the outer wall of the container, a second temperature sensor and a silicon rubber heating sheet are arranged in the dropping liquid pipe heat-insulating layer and the liquid storage tank heat-insulating layer, the controller controls the silicon rubber heating sheet through the second temperature sensor to prevent the cooling and the coagulation of the gel cell mixed liquid and the gel liquid, the gel liquid does not contain microbial cells when existing alone, the storage temperature can be set to be slightly higher without considering cell inactivation, and the gel liquid is low in viscosity and is easier to convey through a peristaltic pump; in actual operation, a plurality of gel liquid storage tanks and a plurality of dropping liquid pipes can be combined and work simultaneously, so that a plurality of corresponding heat-insulating layers, temperature sensors and silicon rubber heating sheets in the heat-insulating layers and the corresponding heat-insulating layers are also provided, and other devices which can be combined and work simultaneously are similar to the heat-insulating layers;

the top cover is arranged at the upper part of the dropping liquid pipe, and a buckle is arranged on the top cover and used for locking and sealing the whole dropping liquid pipe; the top cover is provided with a third connector which is connected with the air conduit and is provided with an air valve, when the dropping liquid pipe stops working, the air valve keeps closed, the top cover is provided with a second connector and a first connector which are respectively connected with the microorganism suspension conduit and the gel liquid conduit, and liquid in the microorganism suspension storage tank and liquid in the gel liquid storage tank are delivered into the dropping liquid pipe through a peristaltic pump to form gel cell mixed liquid, and the mixed temperature of the two is lower than the initial temperature of gel, so that the activity of microorganisms is ensured; the controller controls the blending device on the top cover to blend the gel cell mixed liquid in the dropping liquid pipe, and the insertion depth of the blending device in the dropping liquid pipe can be adjusted (the blending device adopts an electric blending device for a biological laboratory in the prior art, and the adjustment of the installation height is realized by controlling the position of the electric control end of the electric blending device fixed on the bracket, so that the depth adjustment is realized); the lower part of the microorganism suspension storage tank is contacted with a magnetic stirrer, and magnetons are arranged in the tank for stirring to prevent microorganism cells from precipitating;

the controller controls the positive and negative rotation of the air pump wind pressure rotor through a reversing switch connected with the air pump so as to pump air or extract air; when the dropping liquid is dropped in the dropping liquid pipe, the air pump pumps air, one end of the air pump is connected with the air filter to filter air source impurities so as to prevent the gel cell mixed liquid in the dropping liquid pipe from being polluted, the air filter is connected with the air guide pipe through the air flow meter, and the air flow meter adjusts the air pressure in the air guide pipe by adjusting the air leakage flow, so that the dropping speed of the dropping liquid pipe is adjusted; when the individual dropping tube is used, the air valve on the corresponding air conduit is opened, and the air valves corresponding to the rest dropping tubes are closed to prevent air pressure from leaking; when a plurality of dropping pipes are used simultaneously, the time for completing the dropping is different due to different gel mixed liquid or different dropping speeds, and the air valve corresponding to the dropping pipe for completing the dropping is closed in time;

the lower part of the dropping pipe is an inverted cone-shaped opening, the inverted cone-shaped opening is connected with dropping nozzles with different apertures, the aperture of the dropping nozzle determines the size of a final immobilized sphere, an area which is not covered with a dropping pipe heat insulation layer is arranged above the installation position of the dropping nozzle, namely a liquid level detection area, one end of a liquid level sensor which is externally installed sends laser to penetrate through the liquid level detection area to reach a laser receiving area at the other end of the liquid level sensor, and when the liquid level of colloidal gel cells and mixed liquid is higher than that of the liquid level detection area, the laser is scattered due to the Tyndall effect, and the energy reaching the laser receiving area is lower; when the liquid level of the colloidal gel cell mixed liquid is lower than the liquid level detection area, the laser permeability is higher, the energy reaching the laser receiving area is higher, at the moment, the liquid level sensor sends a signal to the controller, the controller controls the peristaltic pump to convey a certain amount of microorganism suspension and gel liquid into the drip tube, meanwhile, the controller controls the air pump to change the pump air into pumping air to enable negative pressure in the drip tube to prevent the gel cell mixed liquid from dripping before being mixed evenly, after the peristaltic pump conducts infusion for a period of time, the controller automatically starts the mixing device, the mixing is stopped after the mixing for a period of time, when the temperature of the drip tube reaches a set temperature, the air pump changes the pumping air into pumping air again from pumping air, if the top cover needs to be opened and then the microorganism suspension is added, the dripping nozzle needs to be blocked in advance to prevent the gel cell mixed liquid from dripping before being mixed evenly, and when the gel cell mixed liquid is mixed, the silicon rubber heating sheet can heat the gel cell mixed liquid; a magnetic stirrer was used to prevent the incompletely gelled spheres in the collector one (collector two) from sticking to each other.

In actual operation, when a large amount of immobilized spheres need to be prepared, a peristaltic pump is needed for automatic liquid supplementation, and a collector (large capacity) is needed for collecting the immobilized spheres. When a small amount of immobilized spheres is prepared, fluid replacement may not be required and a second collector (small volume) is sufficient.

After adopting the structure, the invention has the beneficial effects that:

1. the gel liquid and the microbial cells are directly mixed in the hydrophobic conical dropping liquid tube, so that the residue on the inner wall of the container in the process of preparing the gel cell mixed liquid in the container transferring process is avoided;

2. filtering the compressed air by using an air source filtering device to prevent the gel cell mixed liquid from being polluted by air source impurities;

3. the outer wall of the conical dropping liquid pipe is covered with a heating sleeve, and the gel cell mixed liquid is kept at a constant temperature through a temperature controller so as to prevent viscosity from increasing, so that the dropping speed and the size of a final sphere are stabilized;

4. the air valve combination and the air conduit are used for connecting the single air source with the plurality of conical dropping liquid pipes, so that the preparation of immobilized spheres of the same type of microorganisms can be accelerated when the gel cell mixed liquid is contained, and the preparation of immobilized spheres of various types of microorganisms can be simultaneously carried out when the gel cell mixed liquid is contained;

5. the immobilization time is shortened by refrigeration while the microbial spheres are collected;

6. the preparation of the microorganism immobilized spheres can be continuously carried out by automatically supplementing gel liquid and microorganism cells, so that the labor is saved.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a schematic diagram of the present invention.

Fig. 2 is a schematic view of the internal structure of the present invention.

Fig. 3 is a schematic view of the installation positions of the silicone rubber heating sheet and the second temperature sensor in the present invention.

Fig. 4 is an electrical control block diagram of the present invention.

Description of reference numerals:

the device comprises a cabin shell 1, a liquid dropping nozzle 2, an air conduit 3, an air flow meter 4, an air filter 5, a condenser 6, an air pump 7, a magnetic stirrer 8, a second collector 9, a first collector 10, a compressor 11, a power adapter 12, a controller 13, a liquid storage tank heat-insulating layer 14, a liquid conduit 15, a peristaltic pump 16, a microorganism suspension conduit 17, a gel liquid conduit 18, a buckle 19, a blending machine 20, an air valve 21, a dropping liquid pipe heat-insulating layer 22, a liquid level sensor 23, a cabin door 24, a microorganism suspension storage tank 25, a gel liquid storage tank 26, a dropping liquid pipe 27, a liquid level detection zone 27-1, an inverted cone-shaped port 27-2, a top cover 28, a first interface 28-1, a second interface 28-2, a third interface 28-3, a support 29, a silicon rubber heating sheet 30, a first temperature sensor 31 and a second temperature sensor 32.

Detailed Description

The invention will be further described with reference to the accompanying drawings.

Referring to fig. 1 to 4, the technical solution adopted by the present embodiment is: the device comprises a cabin shell 1, a dropping nozzle 2, an air conduit 3, an air flow meter 4, an air filter 5, a condenser 6, an air pump 7, a magnetic stirrer 8, a first collector 10, a compressor 11, a power adapter 12, a controller 13, a liquid storage tank heat-insulating layer 14, a liquid conduit 15, a peristaltic pump 16, a microorganism suspension conduit 17, a gel liquid conduit 18, a blending machine 20, an air valve 21, a dropping liquid pipe heat-insulating layer 22, a liquid level sensor 23, a microorganism suspension storage tank 25, a gel liquid storage tank 26, a dropping liquid pipe 27, a top cover 28, a silicon rubber heating sheet 30, a first temperature sensor 31 and a second temperature sensor 32; a microorganism suspension storage tank 25 and a gel liquid storage tank 26 are arranged on the right side of the top of the cabin shell 1 in parallel; a plurality of liquid dropping pipes 27 are erected at the top of the outer shell 1 of the cabin body, a top cover 28 is hinged on an opening at the upper end of each liquid dropping pipe 27, and a first connector 28-1, a second connector 28-2 and a third connector 28-3 are sequentially connected on the top cover 28 in a penetrating manner from right to left; the liquid outlet of the microbial suspension storage tank 25 and the liquid outlet of the gel liquid storage tank 26 are both connected with a peristaltic pump 16, the inlet ends of the peristaltic pumps 16 are both connected with liquid guide pipes 15, the liquid guide pipes 15 are respectively arranged in the microbial suspension storage tank 25 and the gel liquid storage tank 26, and the outlet end of the peristaltic pump 16 at the top of the microbial suspension storage tank 25 is connected with a third connector 28-3 through a microbial suspension guide pipe 17; the outlet end of the peristaltic pump 16 at the top of the gel liquid storage tank 26 is connected with a first connector 28-1 through a gel liquid conduit 18; the electric control end of the blending device 20 is movably arranged on the top cover 28 through a bracket, and the stirring end of the blending device 20 is arranged in the dropping liquid pipe 27 after passing through the second connector 28-2; a dropping liquid pipe insulating layer 22 is wrapped outside the dropping liquid pipe 27, a liquid level detection area 27-1 is arranged between the bottom end of the dropping liquid pipe insulating layer 22 and the outlet edge of the inverted cone-shaped opening 27-2 at the bottom of the dropping liquid pipe 27, and a liquid level sensor 23 is arranged outside the liquid level detection area 27-1 (the liquid level sensor 23 is fixed on the upper surface of the top cover 28); the inverted cone-shaped port 27-2 is connected with the drip nozzles 2, the drip nozzles 2 are arranged in the cabin shell 1 after penetrating through the top cover 28, and each drip nozzle 2 is correspondingly arranged at an inlet at the upper end of the first collector 10 in the cabin shell 1; the outer wall of the gel liquid storage tank 26 is wrapped with a liquid storage tank heat-insulating layer 14; a silicon rubber heating sheet 30 and a second temperature sensor 32 are embedded in the liquid storage tank heat-insulating layer 14 and the dropping liquid pipe heat-insulating layer 22; a condenser 6 is arranged in the outer shell 1 of the cabin body, and the first collector 10 is arranged in the condenser 6; a first temperature sensor 31 is fixed on the inner wall of the outer shell 1 of the cabin body; the compressor 11 and the power adapter 12 are arranged inside the cabin shell 1 from bottom to top and are positioned on the right side outside the condenser 6; the controller 13 is embedded on the front wall of the outer shell 1 of the cabin body; the air pump 7 and the air filter 5 are arranged inside the cabin shell 1 from bottom to top and are positioned on the left side outside the condenser 6, wherein the outlet end of the air pump 7 is connected with the air filter 5, the air filter 5 is connected with the air flow meter 4, the air flow meter 4 is embedded on the front wall of the cabin shell 1, the air flow meter 4 is connected with the air conduit 3, the air outlet end of the air conduit 3 is connected with a plurality of air valves 21 through a plurality of branch pipes, and the air outlet end of each air valve 21 is communicated with the third connector 28-3 through a pipeline; the bottoms of the gel liquid storage tank 26 and the first collector 10 are respectively provided with a magnetic stirrer 8, and the power adapter 12 is electrically connected with the condenser 6, the compressor 11, the controller 13, the first temperature sensor 31, the second temperature sensor 32, the silicon rubber heating sheet 30, the liquid level sensor 23, the peristaltic pump 16, the magnetic stirrer 8 and the air pump 7; the first temperature sensor 31, the second temperature sensor 33 and the liquid level sensor 23 are all connected with the controller 13, and the controller 13 is connected with the compressor 11, the magnetic stirrer 8, the blending device 20, the silicon rubber heating sheet 30, the peristaltic pump 16 and the air pump 7.

Furthermore, a buckle 19 is screwed on one side of the top cover 28 away from the hinged end, and the buckle 19 is movably arranged in a clamping groove on the side wall of the dropping liquid pipe 27.

Further, a cabin door 24 is hinged at the front opening of the cabin shell 1.

The working steps of the embodiment are as follows:

1. preparing gel liquid in a dropping liquid pipe 27 and a gel liquid storage tank 26 (if liquid needs to be replenished) which are separated from the respective heat-insulating layers and a top cover 28, heating and dissolving, and inserting into the respective heat-insulating layers (the heat-insulating layers are the dropping liquid pipe heat-insulating layer 22 and the liquid storage tank heat-insulating layer 14, and both adopt heat-insulating sleeves);

2. switching on a power supply;

3. starting the device through the controller 13, setting the working time of the peristaltic pump 16 (if liquid is needed to be supplemented) and the working time of the blending device 20 in the liquid supplementing process, and setting the internal low temperature of the cabin shell 1, the temperature of the liquid storage tank heat-insulating layer 14 (if liquid is needed to be supplemented) and the temperature of the dropping liquid pipe heat-insulating layer 22;

4. opening a hatch door 24, putting the first collector 10, plugging the drip nozzle 2, and closing the hatch door 24;

5. starting an air pump 7 to pump air through a controller 13, adding the microorganism suspension, and closing the top cover 28 and the buckle 19;

6. the blocking of the liquid dropping nozzle 2 is released (the blocking is not needed in the automatic liquid supplementing process);

7. after the blending and heating process is finished, the controller 13 automatically changes the air pump 7 from pumping air to pumping air, and adjusts the air flow meter 4 to control the dropping speed;

8. the air valve 21 corresponding to the dropping pipe 27 where the dropping has ended is closed and the air flow meter 4 is adjusted to stabilize the dropping rate;

9. after the whole liquid dropping process is finished, the working state in the low-temperature cabin shell 1 is kept, and other devices are closed through the controller 13;

10. closing the whole device after the low-temperature immobilization is finished;

11. the power supply is disconnected.

After adopting above-mentioned structure, this embodiment beneficial effect does: the specific embodiment provides a device for preparing microorganism immobilized spheres, which avoids the residue on the inner wall of a container in the process of preparing gel cell mixed liquid in the transfer container; preventing the mixed liquid of the gel cells from being polluted by gas source impurities; the immobilization time is shortened; the preparation is continuously carried out, the labor is saved, and the practicability is stronger.

The first embodiment is as follows:

in this embodiment, the first collector 10 is replaced by the second collector 9, the support 29 is arranged inside the cabin body shell 1, the two second collectors 9 are arranged on the upper side of the transverse plate of the support 29, the two second collectors 9 are arranged on the lower side of the transverse plate of the support 29, the transverse plate of the support 29 is in split connection with the longitudinal plate of the support 29, the transverse plate is detached as required, and the plurality of second collectors 9 are placed so as to facilitate pre-cooling of the coagulants in the collectors in advance.

The above description is only for the purpose of illustrating the technical solutions of the present invention and not for the purpose of limiting the same, and other modifications or equivalent substitutions made by those skilled in the art to the technical solutions of the present invention should be covered within the scope of the claims of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. The utility model provides a microorganism immobilization spheroid preparation facilities which characterized in that: the device comprises a cabin shell (1), a liquid dropping nozzle (2), an air conduit (3), an air flow meter (4), an air filter (5), a condenser (6), an air pump (7), a magnetic stirrer (8), a first collector (10), a compressor (11), a power adapter (12), a controller (13), a liquid storage tank heat-insulating layer (14), a liquid conduit (15), a peristaltic pump (16), a microorganism suspension conduit (17), a gel liquid conduit (18), a blending machine (20), an air valve (21), a liquid dropping pipe heat-insulating layer (22), a liquid level sensor (23), a microorganism suspension storage tank (25), a gel liquid storage tank (26), a liquid dropping pipe (27), a top cover (28), a silicon rubber heating sheet (30), a first temperature sensor (31) and a second temperature sensor (32); a microorganism suspension storage tank (25) and a gel liquid storage tank (26) are arranged on the right side of the top of the cabin shell (1) in parallel; a plurality of liquid dropping pipes (27) are erected at the top of the cabin body shell (1), a top cover (28) is hinged to an opening at the upper end of each liquid dropping pipe (27), and a first connector (28-1), a second connector (28-2) and a third connector (28-3) are sequentially connected to the top cover (28) in a penetrating manner from right to left; the liquid outlet of the microbial suspension storage tank (25) and the liquid outlet of the gel liquid storage tank (26) are both connected with a peristaltic pump (16), the inlet end of the peristaltic pump (16) is connected with a liquid conduit (15), the liquid conduits (15) are respectively arranged in the microbial suspension storage tank (25) and the gel liquid storage tank (26), and the outlet end of the peristaltic pump (16) at the top of the microbial suspension storage tank (25) is connected with a third connector (28-3) through a microbial suspension conduit (17); the outlet end of a peristaltic pump (16) at the top of the gel liquid storage tank (26) is connected with a first interface (28-1) through a gel liquid conduit (18); the electric control end of the blending device (20) is movably arranged on the top cover (28) through a bracket, and the stirring end of the blending device (20) is arranged in the dropping liquid pipe (27) after passing through the second connector (28-2); a dropping liquid pipe heat-insulating layer (22) is wrapped outside the dropping liquid pipe (27), a liquid level detection area (27-1) is arranged between the bottom end of the dropping liquid pipe heat-insulating layer (22) and the outlet edge of an inverted cone-shaped opening (27-2) at the bottom of the dropping liquid pipe (27), and a liquid level sensor (23) is arranged outside the liquid level detection area (27-1); the inverted cone-shaped port (27-2) is connected with a dripping nozzle (2), the dripping nozzle (2) penetrates through the top cover (28) and is arranged inside the cabin shell (1), and each dripping nozzle (2) is correspondingly arranged at an inlet at the upper end of a first collector (10) inside the cabin shell (1); the outer wall of the gel liquid storage tank (26) is wrapped with a liquid storage tank heat-insulating layer (14); a silicon rubber heating sheet (30) and a second temperature sensor (32) are embedded in the liquid storage tank insulating layer (14) and the dropping liquid pipe insulating layer (22); a condenser (6) is arranged in the cabin shell (1), and the first collector (10) is arranged in the condenser (6); a first temperature sensor (31) is fixed on the inner wall of the cabin shell (1); the compressor (11) and the power adapter (12) are arranged inside the cabin shell (1) from bottom to top and are positioned on the right side outside the condenser (6); the controller (13) is embedded on the front wall of the outer shell (1) of the cabin body; the air pump (7) and the air filter (5) are arranged inside the cabin shell (1) from bottom to top and are positioned on the left side outside the condenser (6), wherein the outlet end of the air pump (7) is connected with the air filter (5), the air filter (5) is connected with the air flow meter (4), the air flow meter (4) is embedded on the front wall of the cabin shell (1), the air flow meter (4) is connected with the air conduit (3), the air outlet end of the air conduit (3) is connected with a plurality of air valves (21) through a plurality of branch pipes, and the air outlet end of each air valve (21) is communicated with the third connector (28-3) through a pipeline; the bottom of the gel liquid storage tank (26) and the bottom of the first collector (10) are respectively provided with a magnetic stirrer (8), and the power adapter (12) is electrically connected with the condenser (6), the compressor (11), the controller (13), the first temperature sensor (31), the second temperature sensor (32), the silicon rubber heating sheet (30), the liquid level sensor (23), the peristaltic pump (16), the magnetic stirrer (8) and the air pump (7); the first temperature sensor (31), the second temperature sensor (32) and the liquid level sensor (23) are all connected with a controller (13), and the controller (13) is connected with a compressor (11), a magnetic stirrer (8), a blending device (20), a silicon rubber heating sheet (30), a peristaltic pump (16) and an air pump (7); a buckle (19) is screwed on one side of the top cover (28) far away from the hinged end, and the buckle (19) is movably clamped in a clamping groove in the side wall of the dropping liquid pipe (27); a cabin door (24) is hinged at the front side opening of the cabin shell (1);

the power adapter (12) supplies power to power consumption devices including the controller (13); the controller (13) controls the compressor (11) of the heat exchanger through the first temperature sensor (31), and the condenser (6) enables the inner part of the cabin shell (1) to maintain stable low temperature;

after the dripping nozzle (2) is plugged, gel liquid is directly prepared in a dripping pipe (27) and a gel liquid storage tank (26) which are separated from a respective heat insulation layer and a top cover (28), the gel liquid is heated, dissolved and cooled to a certain temperature, the gel liquid is inserted into the respective heat insulation layer, microorganism suspension liquid is added, the top cover (28) is covered, and after uniform mixing, subsequent dripping is carried out, so that gel residue during secondary transfer is avoided;

in order to prevent the coagulation of gel cell mixed liquid in the dropping pipe (27) and gel liquid in the gel liquid storage tank (26), a dropping pipe heat-insulating layer (22) and a liquid storage tank heat-insulating layer (14) are covered on the outer wall of the container, a second temperature sensor (32) and a silicon rubber heating sheet (30) are arranged in the dropping pipe heat-insulating layer (22) and the liquid storage tank heat-insulating layer (14), and the controller (13) controls the silicon rubber heating sheet (30) through the second temperature sensor (32) to prevent the cooling coagulation of the gel cell mixed liquid and the gel liquid;

a top cover (28) is arranged at the upper part of the dropping pipe (27); the top cover (28) is provided with a third connector (28-3) which is connected with the air conduit (3) and is provided with an air valve (21), when the dripping pipe (27) stops working, the air valve (21) keeps closed, the top cover (28) is provided with a second connector (28-2) and a first connector (28-1) which are respectively connected with the microorganism suspension conduit (17) and the gel liquid conduit (18), and liquid in the microorganism suspension storage tank (25) and the gel liquid storage tank (26) is delivered into the dripping pipe (27) through a peristaltic pump (16) to form a gel cell mixed solution, and the temperature of the mixed solution is lower than the initial temperature of gel, so that the activity of microorganisms is ensured; the controller (13) controls the blending device (20) on the top cover (28) to blend the gel cell mixed solution in the dropping liquid pipe (27); the lower part of the microorganism suspension storage tank (25) is contacted with a magnetic stirrer (8), and magnetons are stirred in the tank to prevent microorganism cells from precipitating;

the controller (13) controls the forward and reverse rotation of a wind pressure rotor of the air pump (7) through a forward and reverse switch connected with the air pump (7) so as to pump air or extract air; when the dropping liquid pipe (27) drops liquid, the air pump (7) pumps air, one end of the air pump (7) is connected with the air filter (5) to filter air source impurities so as to prevent the gel cell mixed liquid in the dropping liquid pipe from being polluted, the air filter (5) is connected with the air conduit (3) through the air flow meter (4), and the air flow meter (4) adjusts the air pressure in the air conduit (3) by adjusting the air leakage flow, so that the dropping speed of the dropping liquid pipe is adjusted; when the individual dropping tube (27) is used, the air valve (21) on the corresponding air conduit (3) is opened, and the air valves (21) corresponding to the rest dropping tubes (27) are closed to prevent the air pressure from leaking; when a plurality of dropping tubes (27) are used simultaneously, the time for completing dropping is different due to different gel mixed liquid or different dropping rates in the dropping tubes, and the air valve (21) corresponding to the dropping tube (27) completing dropping is closed in time;

the lower part of the dropping pipe (27) is an inverted cone-shaped opening (27-2), the inverted cone-shaped opening (27-2) is connected with dropping nozzles (2) with different apertures, the aperture of the dropping nozzle (2) determines the size of a final immobilized sphere, an area which is not covered with a dropping pipe heat-insulating layer (22) is arranged above the installation position of the dropping nozzle (2), namely a liquid level detection area (27-1), one end of a liquid level sensor (23) which is externally installed emits laser which penetrates through the liquid level detection area (27-1) to reach a laser receiving area at the other end of the liquid level sensor (23), when the liquid level of colloidal gel cells and mixed liquid is higher than the liquid level detection area (27-1), the laser is scattered due to the Tyndall effect, and the energy reaching the laser receiving area is lower; when the liquid level of the colloidal gel cell mixed liquid is lower than a liquid level detection area (27-1), the laser permeability is high, the energy reaching a laser receiving area is high, at the moment, a liquid level sensor (23) sends a signal to a controller (13), the controller (13) controls a peristaltic pump (16) to convey a certain amount of microorganism suspension and gel liquid into a dropping liquid pipe (27), meanwhile, the controller (13) controls an air pump (7) to change pump air into suction air to enable the interior of the dropping liquid pipe (27) to be in negative pressure so as to prevent the gel cell mixed liquid from dripping before being mixed evenly, after the peristaltic pump (16) conducts infusion for a period of time, the controller (13) automatically starts a mixing device (20) to stop mixing for a period of time, when the temperature of the dropping liquid pipe (27) reaches a set temperature, the air pump (7) changes the suction air into pump air again, if a top cover (28) needs to be opened and then the microorganism suspension is added, a dripping nozzle (2) needs to be blocked in advance so as to prevent the gel cell mixed liquid from dripping without being mixed evenly, and when the gel cell is mixed liquid, a heating sheet is used for heating the silicon rubber mixed liquid; a magnetic stirrer (8) is used to prevent the incompletely gelled spheres in the first collector (10) from sticking to each other.

2. The apparatus for preparing microorganism-immobilized spheres of claim 1, wherein: the first collector (10) is replaced by a second collector (9), a support (29) is arranged inside the cabin body shell (1), the second collectors (9) are arranged on the upper side and the lower side of a transverse plate of the support (29), and the transverse plate of the support (29) is connected with a longitudinal plate of the support (29) in a split mode.