CN108384773A - A kind of imbedded microbe gelled pill preparation facilities - Google Patents

A kind of imbedded microbe gelled pill preparation facilities Download PDF

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Publication number
CN108384773A
CN108384773A CN201810030654.7A CN201810030654A CN108384773A CN 108384773 A CN108384773 A CN 108384773A CN 201810030654 A CN201810030654 A CN 201810030654A CN 108384773 A CN108384773 A CN 108384773A
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CN
China
Prior art keywords
tank
gelled pill
crosslinking agent
preparation facilities
imbedded microbe
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Pending
Application number
CN201810030654.7A
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Chinese (zh)
Inventor
吴楠
曾明
张伟玉
杨磊
王莉
王葳
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Tianjin Agricultural University
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Tianjin Agricultural University
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Priority to CN201810030654.7A priority Critical patent/CN108384773A/en
Publication of CN108384773A publication Critical patent/CN108384773A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/04Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Dispersion Chemistry (AREA)
  • Biological Treatment Of Waste Water (AREA)

Abstract

The present invention provides a kind of imbedded microbe gelled pill preparation facilities, including embedding medium storage tank, activated sludge storage tank, the activated sludge tank bottom is connected with mixer, the embedding medium tank bottom is connected to mixer by peristaltic pump, the mixer bottom is connected with nozzle, cross-linking reaction tank is equipped with below the nozzle, cross-linking reaction tank side is connected with crosslinking agent storage tank by circulation mechanism, cross-linking reaction tank lower end is equipped with outlet, cleaning tank body is equipped with below the outlet, the cleaning tank body lower end is equipped with gelled pill collection port, the cleaning tank body is connected with water tank.Advantageous effect of the present invention:Convenient for the preparation of gelled pill, and the preparation used time is shorter, mass industrial production easy to implement.The imbedded microbe gelled pill produced can be widely used for sewage disposal.

Description

A kind of imbedded microbe gelled pill preparation facilities
Technical field
The invention belongs to the preparing technical fields of imbedded microbe gelled pill, solidifying more particularly, to a kind of imbedded microbe Glue bead preparation facilities.
Background technology
The currently used method for preparing imbedded microbe gelled pill is drop method, i.e., bacterium solution and gel embedding agent is equal Even mixing, mixed liquor are dropped into crosslinking agent, achieve the purpose that immobilization, are rinsed with water crosslinked fluid later and be can be carried out dirt The processing of water.Although method is simple to operation, cost is more cheap.But flow is more, needs more human cost, the used time compared with It is long, it is difficult to realize mass production.
Invention content
In view of this, the present invention is directed to propose a kind of imbedded microbe gelled pill preparation facilities, to solve the above problems Shortcoming.
In order to achieve the above objectives, the technical proposal of the invention is realized in this way:
A kind of imbedded microbe gelled pill preparation facilities, including embedding medium storage tank, activated sludge storage tank, the activity are dirty Mud tank bottom is connected with mixer, and the embedding medium tank bottom is connected to mixer, the mixer bottom by peristaltic pump Portion is connected with nozzle, is equipped with cross-linking reaction tank below the nozzle, cross-linking reaction tank side is connected with by circulation mechanism Crosslinking agent storage tank, cross-linking reaction tank lower end are equipped with outlet, cleaning tank body, the cleaning are equipped with below the outlet Tank body lower end is equipped with gelled pill collection port, and the cleaning tank body is connected with water tank.
Further, the mixer is static mixer.
Further, the nozzle is umbrella-shaped structure, and lower surface is uniformly provided with multiple spray orifices.
Further, the injection diameter is 1mm.
Further, blender is equipped in the cross-linking reaction tank.
Further, the circulation mechanism includes crosslinking agent inlet pipe, crosslinking agent outlet pipe, and the crosslinking agent storage tank passes through friendship Connection agent outlet pipe is connected to the outlet on the downside of cross-linking reaction tank, and the crosslinking agent storage tank is connected to crosslinking instead by crosslinking agent inlet pipe The import on the upside of tank, the crosslinking agent inlet pipe is answered to be equipped with peristaltic pump, the crosslinking agent outlet pipe is equipped with valve.
Further, valve is equipped at the outlet.
Further, the water tank is connected to the water inlet of cleaning tank body by water inlet pipe, and the water inlet pipe is equipped with Peristaltic pump.
Further, it is additionally provided with discharge outlet in the cleaning tank body, drainpipe, the draining are connected at the discharge outlet Pipe is equipped with valve.
Further, valve is equipped at the gelled pill collection port.
Compared with the existing technology, imbedded microbe gelled pill preparation facilities of the present invention has the advantage that:
Imbedded microbe gelled pill preparation facilities of the present invention is convenient for the preparation of gelled pill in preparation process, And the preparation used time is shorter, mass industrial production easy to implement.The imbedded microbe gelled pill produced can be widely used for dirt Water process.
Description of the drawings
The attached drawing for constituting the part of the present invention is used to provide further understanding of the present invention, schematic reality of the invention Example and its explanation are applied for explaining the present invention, is not constituted improper limitations of the present invention.In the accompanying drawings:
Fig. 1 is the imbedded microbe gelled pill preparation facilities structural schematic diagram described in the embodiment of the present invention;
Fig. 2 be prepared with the imbedded microbe gelled pill preparation facilities device described in the embodiment of the present invention it is solidifying Glue bead.
Reference sign:
1- embedding medium storage tanks;2- activated sludge storage tanks;3- peristaltic pumps;4- mixers;5- nozzles;6- blenders;7- is crosslinked Retort;8- crosslinking agent inlet pipes;9- crosslinking agent storage tanks;10- crosslinking agent outlet pipes;11- valves;12- outlets;13- water tanks; 14- water inlet pipes;15- cleaning tank bodies;16- drainpipes;17- gelled pill collection ports;18- gelled pills.
Specific implementation mode
It should be noted that in the absence of conflict, the feature in embodiment and embodiment in the present invention can phase Mutually combination.
In the description of the present invention, it is to be understood that, term "center", " longitudinal direction ", " transverse direction ", "upper", "lower", The orientation or positional relationship of the instructions such as "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outside" is It is based on the orientation or positional relationship shown in the drawings, is merely for convenience of description of the present invention and simplification of the description, rather than instruction or dark Show that signified device or element must have a particular orientation, with specific azimuth configuration and operation, therefore should not be understood as pair The limitation of the present invention.In addition, term " first ", " second " etc. are used for description purposes only, it is not understood to indicate or imply phase To importance or implicitly indicate the quantity of indicated technical characteristic.The feature for defining " first ", " second " etc. as a result, can To express or implicitly include one or more this feature.In the description of the present invention, unless otherwise indicated, " multiple " It is meant that two or more.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation ", " phase Even ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or be integrally connected;It can Can also be electrical connection to be mechanical connection;It can be directly connected, can also indirectly connected through an intermediary, Ke Yishi Connection inside two elements.For the ordinary skill in the art, above-mentioned term can be understood by concrete condition Concrete meaning in the present invention.
The present invention will be described in detail below with reference to the accompanying drawings and embodiments.
As shown in Figure 1, a kind of imbedded microbe gelled pill preparation facilities, including embedding medium storage tank 1, activated sludge storage tank 2,2 bottom of activated sludge storage tank is connected with mixer 4, and 1 bottom of embedding medium storage tank is connected to mixing by peristaltic pump 3 Device 4,4 bottom of the mixer are connected with nozzle 5, and 5 lower section of the nozzle is equipped with cross-linking reaction tank 7, the cross-linking reaction tank 7 one Side is connected with crosslinking agent storage tank 9 by circulation mechanism, and 7 lower end of cross-linking reaction tank is equipped with outlet 12, the outlet 12 lower sections are equipped with cleaning tank body 15, and 15 lower end of the cleaning tank body is equipped with gelled pill collection port 17, and the cleaning tank body 15 connects It is connected to water tank 13.
The mixer 4 is static mixer.
The nozzle 5 is umbrella-shaped structure, and lower surface is uniformly provided with multiple spray orifices.
The injection diameter is 1mm.
Blender 6 is equipped in the cross-linking reaction tank 7.
The circulation mechanism includes crosslinking agent inlet pipe 8, crosslinking agent outlet pipe 10, and the crosslinking agent storage tank 9 passes through crosslinking agent Outlet pipe 10 is connected to the outlet of 7 downside of cross-linking reaction tank, and the crosslinking agent storage tank 9 is connected to crosslinking by crosslinking agent inlet pipe 8 The import of 7 upside of retort, the crosslinking agent inlet pipe 8 are equipped with peristaltic pump 3, and the crosslinking agent outlet pipe 10 is equipped with valve 11, Realize the recycling of crosslinking agent.
Valve 11 is equipped at the outlet.
The water tank 13 is connected to the water inlet of cleaning tank body 15 by water inlet pipe 16, and the water inlet pipe 14 is equipped with compacted Dynamic pump 3.
It is additionally provided with discharge outlet in the cleaning tank body 15, drainpipe 16, the drainpipe 16 are connected at the discharge outlet It is equipped with valve 11.
Valve 11 is equipped at the gelled pill collection port 17.
The course of work of the present embodiment is as follows:
Gel embedding agent is housed, the gel embedding agent includes PVAC polyvinylalcohol and alginic acid in the embedding medium storage tank 1 Sodium SA, the built-in active sludge of the activated sludge storage tank 2 or specified microorganisms strain, the crosslinking agent storage tank 9 are built-in Crosslinking dose, crosslinking agent is mainly calcium chloride and boric acid.Sludge is directly entered mixer 4, the mixer 4 by its own gravity So that activated sludge and gel embedding agent is sufficiently mixed, so that the gelled pill 18 for being embedded with microorganism is dropped to crosslinking by nozzle 5 In retort 7, in cross-linking reaction tank 7, gelled pill 18 and crosslinking agent reaction cured, the gelled pill 18 after solidification from The outlet 12 of 7 bottom of cross-linking reaction tank is discharged, and falls into cleaning tank body 15 and cleaned, from gelled pill after the completion of cleaning Collection port 17 is discharged.
As shown in Fig. 2, for be prepared with the device embedded microorganism polyvinyl alcohol-sodium alginate gel bead, There is no the polyacrylamide gel of polyvinyl alcohol-sodium alginate gel bead of imbedded microbe and not no imbedded microbe small Ball is the polyvinyl alcohol-sodium alginate gel bead for having embedded microorganism in the vessel of the middle and upper parts Fig. 2 two, and two of middle part are There is no polyvinyl alcohol-sodium alginate gel bead of imbedded microbe, lower part be two not no imbedded microbes polypropylene Acrylamide gel bead.Finally observe that the grain size for the bead being prepared by the device is consistent, and regular shape, the dress It sets and is suitble to a variety of small ball materials of hydrogel of synthesis.
The removal efficiency conclusive table of the gelled pill OTC and COD of microorganism are embedded under the conditions of the different water flows of table 1
Through overtesting, it is found that the polyvinyl alcohol for being embedded with microorganism and sodium alginate gel that will be prepared using the device are small Ball is used to handle the terramycin (OTC) in water body, can preferably remove the terramycin in water, the results are shown in Table 1, with dampening The reduction of flow is flowed, water outlet OTC concentration is gradually reduced, and due to adding co-metabolic substance sucrose, OTC removal efficiency increases to 37%, the removal efficiency of COD (COD) reaches as high as 90%.
The gelled pill of 2 activated sludge of table and imbedded microbe is than endogenous respiration rate and maximum specific exogenous respiration rate Contrast table
Note:1Matrix A is that only OTC is carbon source;2Matrix B is existing OTC has sucrose for carbon source again
In addition, also research measurement is carried out to microbial respiratory rate, to evaluate influence of the embedding to microbial activity.It was found that Embedding can dramatically increase the respiratory rate of microorganism, i.e. microbial activity.The results are shown in Table 2, and the ratio of gelled pill is endogenous to exhale It inhales rate and maximum external source respiratory rate is all much larger than loose activated sludge, and imbedded microbe is most after sucrose is added Big specific exogenous respiration rate reaches maximum value.Specific data are shown in Table 2.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention With within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention god.

Claims (10)

1. a kind of imbedded microbe gelled pill preparation facilities, it is characterised in that:Including embedding medium storage tank, activated sludge storage tank, The activated sludge tank bottom is connected with mixer, and the embedding medium tank bottom is connected to mixer by peristaltic pump, institute It states mixer bottom and is connected with nozzle, be equipped with cross-linking reaction tank below the nozzle, cross-linking reaction tank side passes through cycle Mechanism is connected with crosslinking agent storage tank, and cross-linking reaction tank lower end is equipped with outlet, purge tank is equipped with below the outlet Body, the cleaning tank body lower end are equipped with gelled pill collection port, and the cleaning tank body is connected with water tank.
2. a kind of imbedded microbe gelled pill preparation facilities according to claim 1, it is characterised in that:The mixer For static mixer.
3. a kind of imbedded microbe gelled pill preparation facilities according to claim 1, it is characterised in that:The nozzle is Umbrella-shaped structure, and lower surface is uniformly provided with multiple spray orifices.
4. a kind of imbedded microbe gelled pill preparation facilities according to claim 3, it is characterised in that:The spray orifice is straight Diameter is 1mm.
5. a kind of imbedded microbe gelled pill preparation facilities according to claim 1, it is characterised in that:The crosslinking is anti- It answers and is equipped with blender in tank.
6. a kind of imbedded microbe gelled pill preparation facilities according to claim 1, it is characterised in that:The circulator Structure includes crosslinking agent inlet pipe, crosslinking agent outlet pipe, and the crosslinking agent storage tank is connected to by crosslinking agent outlet pipe under cross-linking reaction tank The outlet of side, the crosslinking agent storage tank are connected to the import on the upside of cross-linking reaction tank, the crosslinking agent by crosslinking agent inlet pipe Inlet pipe is equipped with peristaltic pump, and the crosslinking agent outlet pipe is equipped with valve.
7. a kind of imbedded microbe gelled pill preparation facilities according to claim 1, it is characterised in that:The outlet Place is equipped with valve.
8. a kind of imbedded microbe gelled pill preparation facilities according to claim 1, it is characterised in that:The water tank is logical The water inlet that water inlet pipe is connected to cleaning tank body is crossed, and the water inlet pipe is equipped with peristaltic pump.
9. a kind of imbedded microbe gelled pill preparation facilities according to claim 1 or 9, it is characterised in that:It is described clear It is additionally provided with discharge outlet on cleaning of evaporator body, drainpipe is connected at the discharge outlet, the drainpipe is equipped with valve.
10. a kind of imbedded microbe gelled pill preparation facilities according to claim 1, it is characterised in that:The gel Valve is equipped at bead collection port.
CN201810030654.7A 2018-01-12 2018-01-12 A kind of imbedded microbe gelled pill preparation facilities Pending CN108384773A (en)

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Application Number Priority Date Filing Date Title
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108998372A (en) * 2018-09-30 2018-12-14 兰州大学 A kind of microbial immobilized sphere preparation facilities
CN114698681A (en) * 2022-04-06 2022-07-05 韶关学院 Device and method for manufacturing ozone-rich gel water ball and gel water ball

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008004292A1 (en) * 2006-07-06 2008-01-10 Hitachi Plant Technologies, Ltd. Inclusive immobilization support, wastewater disposal apparatus and wastewater disposal method using the inclusive immobilization support
CN101519632A (en) * 2009-04-01 2009-09-02 天津大学 Device and technology for preparing engineering embedding fixing microorganism spheric bodies
CN106947754A (en) * 2017-03-28 2017-07-14 天津科技大学 A kind of High efficient encapsulation microorganism gel and preparation method thereof
CN107446911A (en) * 2017-07-28 2017-12-08 华南理工大学 A kind of microorganism embedded immobilization bead and its method and special purpose device and application

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008004292A1 (en) * 2006-07-06 2008-01-10 Hitachi Plant Technologies, Ltd. Inclusive immobilization support, wastewater disposal apparatus and wastewater disposal method using the inclusive immobilization support
CN101519632A (en) * 2009-04-01 2009-09-02 天津大学 Device and technology for preparing engineering embedding fixing microorganism spheric bodies
CN106947754A (en) * 2017-03-28 2017-07-14 天津科技大学 A kind of High efficient encapsulation microorganism gel and preparation method thereof
CN107446911A (en) * 2017-07-28 2017-12-08 华南理工大学 A kind of microorganism embedded immobilization bead and its method and special purpose device and application

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108998372A (en) * 2018-09-30 2018-12-14 兰州大学 A kind of microbial immobilized sphere preparation facilities
CN108998372B (en) * 2018-09-30 2023-04-11 兰州大学 Microorganism immobilized sphere preparation device
CN114698681A (en) * 2022-04-06 2022-07-05 韶关学院 Device and method for manufacturing ozone-rich gel water ball and gel water ball
CN114698681B (en) * 2022-04-06 2023-06-23 韶关学院 Device and method for manufacturing gel water ball rich in ozone and gel water ball

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