CN108998372A - A kind of microbial immobilized sphere preparation facilities - Google Patents
A kind of microbial immobilized sphere preparation facilities Download PDFInfo
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- CN108998372A CN108998372A CN201811156048.6A CN201811156048A CN108998372A CN 108998372 A CN108998372 A CN 108998372A CN 201811156048 A CN201811156048 A CN 201811156048A CN 108998372 A CN108998372 A CN 108998372A
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Abstract
A kind of microbial immobilized sphere preparation facilities, the present invention relates to microorganisms technical fields；Right side of cabin cover top portion or so is provided with microorganism suspension holding vessel and coagulant liquid holding vessel side by side；The top shelf of cabin shell is equipped with several minim pipettes, and top cover is hinged in the upper end opening of minim pipette, successively penetrates through from right to left on top cover and is connected with No.1 interface, No. two interfaces and No. three interfaces；The liquid outlet of microorganism suspension holding vessel and the liquid outlet of coagulant liquid holding vessel are respectively connected with peristaltic pump, and the arrival end of peristaltic pump is respectively connected with liquid conduits, and liquid conduits are respectively placed in microorganism suspension holding vessel and coagulant liquid holding vessel.It avoids preparing residual of gel cell mixture during transfer vessel on container inner wall；Prevent gel cell mixture from being polluted by gas source impurity；Shorten the time of immobilization；Preparation is carried out continuously, and is saved manually, practicability is stronger.
Description
Technical field
The present invention relates to microorganisms technical fields, and in particular to a kind of microbial immobilized sphere preparation facilities.
Background technique
In Industrial Wastewater Treatment application, microbial cell immobilization technology is a kind of very promising application technology.
The loss that efficient degrading bacteria can be effectively reduced in conjunction with the superiority bacteria spp for the predetermined substance that can degrade by immobilization mitigates toxic useless
Water poisons the biology of microorganism, improves the degradation efficiency to pollutant.The method of immobilization common at present has absorption method, packet
Bury method, covalent coupling method, cross-linking method.Wherein investment has preferable comprehensive performance, and the survival rate of cell is higher, and embeds body
It is flexible in application in reaction engineering, become the process for fixation being most widely used in entire immobilized microorganism technique.Embedding
Method is in the network structure of gel or to be wrapped in cell encapsulation in semipermeable polymeric film, small point using the method for physics
The substrate and product of son can freely enter and leave, and microorganism is without leakage.Polyvinyl alcohol (PVA) is good hydrophilic because having
Property, chemical stability and mechanical performance, Resistance to microbes ability, which becomes by force in sewage treatment investment in recent years, to be widely applied
One of immobilization material.It is the most common method in carrier for preparing that irreversible plasticizing, which occurs, with boric acid crosslinking in it.But with this
It is excessive that it often is faced with PVA viscosity during material production immobilization sphere, easily condenses, and drippage gel need to be in 4 °C of coolings etc.
Problem.
Immobilized cell is obtained different depending on the application with the difference of preparation method, can be granular, blocky, item
Shape, film-form or irregular etc..Currently, immobilized cell prepares the bead that granulates mostly, because of the fixation of irregular shape
Change cell it is easy to wear, vulnerable to compressive strain especially in column reactor in reactor, flow velocity is bad, and use it is spherical can
To overcome these disadvantages.In addition, round bead is since its surface area is larger, and it is larger with substrate contact area, so production efficiency
It is relatively high.The catalytic activity of immobilized cell is concentrated mainly on gel surface layer, so the size of gel particle or gel piece must
Its reaction rate is so influenced, now thinks that the most suitable size of gel particle of investment preparation should be 2-3mm, so obtaining efficient
It is particularly significant during pollutant process quickly to prepare uniform immobilization sphere.
" drop method " is that laboratory prepares that ball shape fixation microorganism is fairly simple, common method.Currently used drop
The method that the method for falling prepares bead has two classes: the first kind is wriggling syringe pump method, peristaltic pump be one kind can timing, quantitative, two-way drawing liquid and
The combination pump of liquid feeding pressurization.The pump rolls silicone rubber tube using multiple followers of pump head, thus the gel in silicone rubber tube
Cell mixture interruption squeezes out to constant current nozzle, instills in the container equipped with forming agent, forms uniform sphere.With this
Although method speed regulation is convenient, speed is fast, sphere system easy to control in size, can be used for the production of high-volume spheroid carrier, due to mixing
Liquid viscosity is larger to cause tube wall residual quantity big, and be directed to different liquid storages when peristaltic pump and minim pipette cannot share, need to configure multiple
Peristaltic pump and minim pipette correspond, otherwise can composite pollution；Second class is air pressure method, and gel cell mixture is fallen
Air pressurized, mixed liquor instill in forming agent from the thin mouth drippage of the other end, bead can be obtained after entering closed container, but
Fail to effectively utilize single gas source in drip process, is only applicable to a small amount of immobilization sphere preparation of single.In addition, this method
Be related to compressed air but without gas source filter device, sample is easily polluted by gas source impurity, meanwhile, Compound mixed solution process need from
Open-top receptacle is transferred to closed container, and more gel residue is had because viscosity is larger, in container.
Meanwhile when actually preparing gel cell mixture, microbial cell suspension is usually to be higher than room temperature but be lower than
Microorganism does not tolerate and coagulant liquid is added under conditions of temperature, and the mixed liquor prepared at this time is not easy to condense, viscosity is lower and easy
Drippage, in subsequent drip process gel cell mixture can be with the reduction of temperature and viscosity becomes larger, to influence to drip rate.
Both the above method all not can effectively solve this problem.In addition, the sphere of drip shaping is usually also needed to be transferred to 4 °C and be placed
It is night, time-consuming and laborious more preferably to solidify.
Summary of the invention
In view of the defects and deficiencies of the prior art, the present invention intends to provide a kind of structure is simple, design rationally, make
With convenient microbial immobilized sphere preparation facilities, avoid preparing gel cell mixture container during transfer vessel
Residual on inner wall；Prevent gel cell mixture from being polluted by gas source impurity；Shorten the time of immobilization；Preparation is carried out continuously,
It saves manually, practicability is stronger.
To achieve the above object, the technical solution adopted by the present invention is that: it include cabin shell, dropping liquid mouth, air conduit,
Air flow meter, air filter, condenser, air pump, magnetic stirring apparatus, No.1 collector, compressor, power supply adaptor,
Controller, fluid reservoir insulating layer, liquid conduits, peristaltic pump, microorganism suspension conduit, gel fluid catheter, vortex mixer, air valve,
Minim pipette insulating layer, liquid level sensor, microorganism suspension holding vessel, coagulant liquid holding vessel, minim pipette, top cover, silicon rubber heating
Piece, No.1 temperature sensor, No. two temperature sensors；Right side of cabin cover top portion or so is provided with the storage of microorganism suspension side by side
Deposit tank and coagulant liquid holding vessel；The top shelf of cabin shell is equipped with several minim pipettes, is hinged with top in the upper end opening of minim pipette
It covers, successively perforation is connected with No.1 interface, No. two interfaces and No. three interfaces from right to left on top cover；Microorganism suspension holding vessel
The liquid outlet of liquid outlet and coagulant liquid holding vessel is respectively connected with peristaltic pump, and the arrival end of peristaltic pump is respectively connected with liquid conduits,
Liquid conduits are respectively placed in microorganism suspension holding vessel and coagulant liquid holding vessel, wherein at the top of microorganism suspension holding vessel
The outlet end of peristaltic pump is connect by microorganism suspension conduit with No. three interfaces；The outlet of peristaltic pump at the top of coagulant liquid holding vessel
End is connect by gel fluid catheter with No.1 interface；The automatically controlled end of vortex mixer is movably arranged on top cover by bracket, vortex mixer
Stirring end pass through No. two interfaces after, be set to inside minim pipette；The external packet of minim pipette is equipped with minim pipette insulating layer, the dropping liquid
It is level sensing area between the bottom end of pipe insulating layer and the back taper mouth outlet edge of dropping liquid bottom of the tube, outside the level sensing area
Side is equipped with liquid level sensor；It is connected with dropping liquid mouth on the back taper mouth and is set to cabin shell after dropping liquid mouth passes through top cover
Inside, and each dropping liquid mouth is correspondingly arranged in the upper end inlet of the No.1 collector of cabin interior of shell；The gel
The outer wall packet of liquid holding vessel is equipped with fluid reservoir insulating layer；It is embedded in the fluid reservoir insulating layer and minim pipette insulating layer
Silicon rubber heating sheet and No. two temperature sensors；The inside of the cabin shell is equipped with condenser, the No.1 collector
It is set to the inside of condenser；The inner wall of cabin shell is fixed with No.1 temperature sensor；The compressor, power supply adaptor
It is all set in the inside of cabin shell from the bottom to top, and is located on the right side of condenser external；The controller is embedded outside cabin
On the antetheca of shell；The air pump, air filter are all set in the inside of cabin shell from the bottom to top, and are located at condenser
The outlet end of outer left side, air pump is connect with air filter, and air filter is connect with air flow meter, air stream
Meter is entrenched on the antetheca of cabin shell, and air conduit is connected in air flow meter, and the outlet side of air conduit passes through number
A branch pipe is connected with several air valves, and the outlet side of each air valve passes through pipeline and No. three interface perforations connect；Described
The bottom of coagulant liquid holding vessel and No.1 collector is equipped on magnetic stirring apparatus, the power supply adaptor and condenser,
Compressor, controller, No.1 temperature sensor, No. two temperature sensors, silicon rubber heating sheet, liquid level sensor, peristaltic pump, magnetic
Power blender and air pump electrical connection；No.1 temperature sensor, No. two temperature sensors 33 and liquid level sensor with control
Device connection processed, controller are connect with compressor, magnetic stirring apparatus, vortex mixer, silicon rubber heating sheet, peristaltic pump and air pump.
Further, the No.1 collector replaces with No. two collectors, and cabin interior of shell is equipped with bracket, and several two
Number collector is set to the two sides up and down of the transverse slat of bracket, is split type connection between the transverse slat of bracket and the stringer board of bracket, can
Multiple No. two collectors are put into, convenient for coagulator in collector is pre-chilled in advance.
Further, the side on the top cover far from hinged end is screwed with buckle, and buckle activity A Ka society is in dropping liquid
In the card slot of pipe side wall.
Further, hatch door is hinged at the front openings of the cabin shell.
The working principle of the invention: power supply adaptor is provides electric power with electric original copy including controller；Controller
It controls the compressor of heat exchanger by No.1 temperature sensor, and makes to maintain stable low in cabin shell by condenser
Temperature；
To reduce the residual of coagulant liquid and gel cell mixture in respective line, the hydrophobic material in all parts contacted
Material production.After dropping liquid mouth is blocked, coagulant liquid is directly prepared in the minim pipette and coagulant liquid for being detached from respective insulating layer and top cover
In holding vessel, it is cooled to certain temperature after heating for dissolving, is inserted into respective insulating layer, microbial suspension is added, covers top
It covers and locking buckles, after mixing, carry out subsequent dropping liquid, avoid gel residue when secondary transferring；
The condensation of minim pipette inner gel cell mixture and coagulant liquid holding vessel inner gel liquid in order to prevent, covers on container outer wall
Covered minim pipette insulating layer and fluid reservoir insulating layer, have in minim pipette insulating layer and fluid reservoir insulating layer No. two temperature sensors and
Silicon rubber heating sheet, controller control silicon rubber heating sheet by No. two temperature sensors, prevent gel cell mixture and coagulate
Glue cooling condensation, microbial cell is not contained when coagulant liquid individualism, without considering cell inactivation so can will store
Temperature setting it is little higher, such viscosity is smaller to be conveyed more easily by peristaltic pump；In practical operation, coagulant liquid holding vessel
With minim pipette can have it is multiple combine, work at the same time, so corresponding insulating layer and its interior temperature sensor and silicon rubber
Heating sheet also has multiple, other are similar in combination with devices for working at the same time；
Top cover is arranged at minim pipette top, has buckle on top cover for locking and seals entire minim pipette；There are No. three interfaces on top cover, with
Air conduit connects and has air valve, and when minim pipette stops working, air valve is remained turned-off, have on top cover No. two interfaces and
No.1 interface is connect with microorganism suspension conduit and gel fluid catheter respectively, and passes through peristaltic pump for microorganism suspension holding vessel
With liquid delivery in coagulant liquid holding vessel into minim pipette, gel cell mixture is formed, the mixed temperature of the two can be lower than
The initial temperature of gel, to guarantee the activity of microorganism；Controller controls the vortex mixer on top cover to the gel in minim pipette
Cell mixture is mixed, and the depth that vortex mixer is inserted into minim pipette is adjustable, and (vortex mixer use is given birth in the prior art
The electronic vortex mixer of object use for laboratory is fixed on the position on bracket by controlling its automatically controlled end and realizes the tune of mounting height
Section, to reach depth adjustment)；Contacted below microorganism suspension holding vessel with magnetic stirring apparatus, there is magneton to stir in tank to prevent
Only microbial cell precipitates；
Controller controls the positive and negative rotation of air pump wind pressure rotor by the two-direction switch connecting with air pump to realize pump gas or pumping
Gas；In minim pipette dropping liquid, air pump pumps gas, and air pump one end is connected to filter gas source impurity to prevent dirt with air filter
The gel cell mixture in minim pipette is contaminated, air filter is connected by air flow meter with air conduit, air flow meter
By adjusting air drainage amount to adjust the air pressure in air conduit, and then adjust the rate of minim pipette dropping liquid；When making
When with individual minim pipettes, the air valve on corresponding air conduit is opened, and the corresponding air valve of remaining minim pipette is closed, to prevent
Air pressure leaks；When multiple minim pipettes simultaneously in use, due to its inner gel mixed liquor how much different or drop velocities
Difference, the time for causing dropping liquid to be completed is different, and the corresponding air valve of minim pipette for completing dropping liquid is closed in time；
Minim pipette lower part is back taper mouth, and back taper mouth connects the dropping liquid mouth of different pore size, and the aperture of dropping liquid mouth determines final solid
Surely change the size of sphere, have the region for not covering minim pipette insulating layer, i.e. level sensing area above dropping liquid mouth installation site,
The laser that one end of the liquid level sensor of outside installation issues the laser light level sensing area arrival liquid level sensor other end connects
By region, when the liquid level of the gel cell of colloidal and mixed liquor is higher than level sensing area, due to Tyndall effect, laser quilt
Scattering, the energy for reaching laser region of acceptance are lower；When the liquid level of the gel cell mixture of colloidal is lower than level sensing area
When, the permeability of laser is higher, and the energy for reaching laser region of acceptance is higher, believes at this point, liquid level sensor is sent to controller
Number, controller control peristaltic pump conveys quantitative microorganism suspension and coagulant liquid into minim pipette, meanwhile, controller controls air
Pump, which becomes pumping from pump gas, to be made just to drip before in negative pressure to prevent gel cell mixture from not mixing in minim pipette, works as peristaltic pump
It is infused after a period of time, controller starts vortex mixer automatically, stops after mixing a period of time, when minim pipette temperature reaches setting temperature
When spending, air pump is become pumping gas again from pumping, and microorganism suspension is then added if necessary to open top cover, then in advance need to be by dropping liquid
Mouth is blocked to prevent gel cell mixture from not mixing and just drip, and gel cell mixture is when being mixed, silicon rubber heating sheet
It can be completed to heat；The sphere of incomplete gelation No.1 collector (No. two collectors) Nei is prevented using magnetic stirring apparatus
Stick to each other.
In practical operation, when needing to prepare a large amount of immobilization spheres, peristaltic pump is needed to carry out automatic liquid supply, it is also necessary to use
No.1 collector (large capacity) collects immobilization sphere.When preparing a small amount of immobilization sphere, it may not be necessary to fluid infusion and
It is sufficient using No. two collectors (low capacity).
After adopting the above structure, the invention has the following beneficial effects:
1, coagulant liquid and microbial cell directly mix in hydrophobic taper minim pipette, avoid and prepare gel cell mixture
Residual during transfer vessel on container inner wall；
2, compressed air is filtered using gas source filter device, prevents gel cell mixture from being polluted by gas source impurity；
3, taper dropping liquid pipe outer wall cover heating mantle and by temperature controller make gel cell mixture holding constant temperature to prevent
Viscosity becomes larger, thus steady drip rate and final sphere size；
4, it is combined by air door and single gas source is connected with multiple taper minim pipettes with air conduit, it is thin to hold gel of the same race
The preparation that can accelerate same species of microorganism immobilization sphere when born of the same parents' mixed liquor, can be same when holding not gel cell mixture of the same race
Production of the Shi Jinhang to multiple-microorganism immobilization sphere；
5, shorten the time of immobilization by refrigeration while collecting microorganism sphere；
6, the preparation of microbial immobilized sphere can be made to be carried out continuously by being automatically replenished coagulant liquid and microbial cell, saved
Manually.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
It obtains other drawings based on these drawings.
Fig. 1 is structural schematic diagram of the invention.
Fig. 2 is schematic diagram of internal structure of the invention.
Fig. 3 is the schematic view of the mounting position of silicon rubber heating sheet and No. two temperature sensors in the present invention.
Fig. 4 is automatically controlled block diagram of the invention.
Description of symbols:
Cabin shell 1, dropping liquid mouth 2, air conduit 3, air flow meter 4, air filter 5, condenser 6, air pump 7, magnetic force
Blender 8, No. two collectors 9, No.1 collector 10, compressor 11, power supply adaptor 12, controller 13, fluid reservoir insulating layers
14, liquid conduits 15, peristaltic pump 16, microorganism suspension conduit 17, gel fluid catheter 18, buckle 19, vortex mixer 20, air valve
21, minim pipette insulating layer 22, liquid level sensor 23, hatch door 24, microorganism suspension holding vessel 25, coagulant liquid holding vessel 26, dropping liquid
Pipe 27, level sensing area 27-1, back taper mouth 27-2, top cover 28, No.1 interface 28-1, No. two interface 28-2, No. three interface 28-
3,31, No. two bracket 29, silicon rubber heating sheet 30, No.1 temperature sensor temperature sensors 32.
Specific embodiment
The present invention will be further described below with reference to the drawings.
Referring to as Figure 1-Figure 4, present embodiment the technical solution adopted is that: it includes cabin shell 1, dropping liquid
Mouth 2, air conduit 3, air flow meter 4, air filter 5, condenser 6, air pump 7, magnetic stirring apparatus 8, No.1 collector
10, compressor 11, power supply adaptor 12, controller 13, fluid reservoir insulating layer 14, liquid conduits 15, peristaltic pump 16, microorganism are outstanding
Fluid catheter 17, gel fluid catheter 18, vortex mixer 20, air valve 21, minim pipette insulating layer 22, liquid level sensor 23, microorganism are outstanding
Liquid holding vessel 25, coagulant liquid holding vessel 26, minim pipette 27, top cover 28, silicon rubber heating sheet 30, No.1 temperature sensor 31, two
Number temperature sensor 32；The right side or so at 1 top of cabin shell is provided with microorganism suspension holding vessel 25 and coagulant liquid storage side by side
Deposit tank 26；The top shelf of cabin shell 1 is equipped with several minim pipettes 27, is hinged with top cover 28 in the upper end opening of minim pipette 27, pushes up
Successively perforation is connected with No.1 interface 28-1, No. two interface 28-2 and No. three interface 28-3 from right to left on lid 28；Microorganism is outstanding
The liquid outlet of liquid holding vessel 25 and the liquid outlet of coagulant liquid holding vessel 26 are respectively connected with peristaltic pump 16, the arrival end of peristaltic pump 16
Liquid conduits 15 are respectively connected with, liquid conduits 15 are respectively placed in microorganism suspension holding vessel 25 and coagulant liquid holding vessel 26,
Wherein the outlet end of the peristaltic pump 16 at 25 top of microorganism suspension holding vessel passes through microorganism suspension conduit 17 and No. three interface 28-
3 connections；The outlet end of the peristaltic pump 16 at 26 top of coagulant liquid holding vessel is connect by gel fluid catheter 18 with No.1 interface 28-1；
The automatically controlled end of vortex mixer 20 is movably arranged on top cover 28 by bracket, and the stirring end of vortex mixer 20 passes through No. two interface 28-2
Afterwards, it is set to inside minim pipette 27；The external packet of minim pipette 27 is equipped with minim pipette insulating layer 22, the bottom of the minim pipette insulating layer 22
It is level sensing area 27-1 between end and the back taper mouth 27-2 outlet edge of 27 bottom of minim pipette, level sensing area 27-1's
Outside is equipped with liquid level sensor 23(liquid level sensor 23 and is fixed on 28 upper surface of top cover)；Connect on the back taper mouth 27-2
Dropping liquid mouth 2 is connected to be set to inside cabin shell 1, and each dropping liquid mouth 2 is correspondingly arranged in after dropping liquid mouth 2 passes through top cover 28
The upper end inlet of No.1 collector 10 inside cabin shell 1；The outer wall packet of the coagulant liquid holding vessel 26 is equipped with liquid storage
Tank insulating layer 14；Silicon rubber heating sheet 30 and two are embedded in the fluid reservoir insulating layer 14 and minim pipette insulating layer 22
Number temperature sensor 32；The inside of the cabin shell 1 is equipped with condenser 6, and the No.1 collector 10 is set to condensation
The inside of device 6；The inner wall of cabin shell 1 is fixed with No.1 temperature sensor 31；The compressor 11, power supply adaptor 12 by
Under the supreme inside for being all set in cabin shell 1, and be located at the outside of condenser 6 right side；The controller 13 is embedded in cabin
On the antetheca of shell 1；The air pump 7, air filter 5 are all set in the inside of cabin shell 1 from the bottom to top, and are located at
The outlet end of 6 outer left side of condenser, air pump 7 is connect with air filter 5, air filter 5 and air flow meter 4
Connection, air flow meter 4 are entrenched on the antetheca of cabin shell 1, and air conduit 3, air conduit are connected in air flow meter 4
3 outlet side is connected with several air valves 21 by several branch pipes, and the outlet side of each air valve 21 passes through pipeline and No. three
Interface 28-3 perforation connection；The bottom of the coagulant liquid holding vessel 26 and No.1 collector 10 is equipped with magnetic stirring apparatus 8
On, the power supply adaptor 12 and 31, No. two condenser 6, compressor 11, controller 13, No.1 temperature sensor temperature pass
Sensor 32, silicon rubber heating sheet 30, liquid level sensor 23, peristaltic pump 16, magnetic stirring apparatus 8 and air pump 7 are electrically connected；No.1
31, No. two temperature sensors 33 of temperature sensor and liquid level sensor 23 are connect with controller 13, controller 13 and compression
Machine 11, magnetic stirring apparatus 8, vortex mixer 20, silicon rubber heating sheet 30, peristaltic pump 16 and air pump 7 connect.
Further, the side on the top cover 28 far from hinged end is screwed with buckle 19, buckles 19 activity A Ka societies
In the card slot of 27 side wall of minim pipette.
Further, hatch door 24 is hinged at the front openings of the cabin shell 1.
The work step of present embodiment is as follows:
1, the minim pipette 27 and coagulant liquid holding vessel 26(of respective insulating layer and top cover 28 are being detached from if necessary to fluid infusion) in prepare
Coagulant liquid, after heating for dissolving, is inserted into respective insulating layer that (above-mentioned insulating layer refers to that minim pipette insulating layer 22 and fluid reservoir are protected
Warm layer 14 both uses muff)；
2, power on；
3, pass through 13 starter of controller and be arranged during fluid infusion the operating time (if necessary to fluid infusion) of peristaltic pump 16 and
The operating time of vortex mixer 20, the inner cryogenic that cabin shell 1 is arranged, fluid reservoir insulating layer 14(are if necessary to fluid infusion) and dropping liquid
The temperature of pipe insulating layer 22；
4, hatch door 24 is opened, No.1 collector 10 is put into, blocks dropping liquid mouth 2, closes hatch door 24；
5, start air pump 7 by controller 13 to be evacuated, microorganism suspension is added and closes top cover 28 and buckle 19；
6, the closure (no longer needing to block during automatic liquid supply hereafter) to dropping liquid mouth 2 is released；
7, mix and heating process after controller 13 automatically by air pump 7 from pumping become pump gas, adjust air flow meter 4
To control drop velocity；
8, it closes the corresponding air valve 21 of minim pipette 27 that dropping liquid has terminated and adjusts air flow meter 4 to stablize drop velocity；
9, it after entire dropping liquid process, keeps the working condition in the cabin shell 1 of low temperature and it is closed by controller 13
Remaining device；
10, to low temperature immobilization after close whole device；
11, power supply is disconnected.
After adopting the above structure, present embodiment has the beneficial effect that present embodiment provides a kind of microorganism
Immobilization sphere preparation facilities avoids preparing residual of gel cell mixture during transfer vessel on container inner wall；
Prevent gel cell mixture from being polluted by gas source impurity；Shorten the time of immobilization；Preparation is carried out continuously, and is saved manually, practical
Property is stronger.
Embodiment one:
No.1 collector 10 is replaced with into No. two collectors 9 in the present embodiment, is equipped with bracket 29 inside cabin shell 1, two two
Number collector 9 is set to the upside of the transverse slat of bracket 29, and two No. two collectors 9 are set to the downside of the transverse slat of bracket 29, props up
It is split type connection between the transverse slat of frame 29 and the stringer board of bracket 29, as needed removes transverse slat, multiple No. two receipts being put into
Storage 9, convenient for coagulator in collector is pre-chilled in advance.
The above is only used to illustrate the technical scheme of the present invention and not to limit it, and those of ordinary skill in the art are to this hair
The other modifications or equivalent replacement that bright technical solution is made, as long as it does not depart from the spirit and scope of the technical scheme of the present invention,
It is intended to be within the scope of the claims of the invention.
Claims (5)
1. a kind of microbial immobilized sphere preparation facilities, it is characterised in that: it includes cabin shell (1), dropping liquid mouth (2), sky
Airway (3), air flow meter (4), air filter (5), condenser (6), air pump (7), magnetic stirring apparatus (8), No.1
Collector (10), compressor (11), power supply adaptor (12), controller (13), fluid reservoir insulating layer (14), liquid conduits
(15), peristaltic pump (16), microorganism suspension conduit (17), gel fluid catheter (18), vortex mixer (20), air valve (21), dropping liquid
Pipe insulating layer (22), liquid level sensor (23), microorganism suspension holding vessel (25), coagulant liquid holding vessel (26), minim pipette (27),
Top cover (28), silicon rubber heating sheet (30), No.1 temperature sensor (31), No. two temperature sensors (32)；Cabin shell (1) top
The right side in portion or so is provided with microorganism suspension holding vessel (25) and coagulant liquid holding vessel (26) side by side；The top of cabin shell (1)
Portion is provided with several minim pipettes (27), is hinged in the upper end opening of minim pipette (27) top cover (28), on top cover (28) extremely by the right side
Left successively perforation is connected with No.1 interface (28-1), No. two interfaces (28-2) and No. three interfaces (28-3)；The storage of microorganism suspension
The liquid outlet of tank (25) and the liquid outlet of coagulant liquid holding vessel (26) are respectively connected with peristaltic pump (16), the entrance of peristaltic pump (16)
End is respectively connected with liquid conduits (15), and liquid conduits (15) are respectively placed in microorganism suspension holding vessel (25) and coagulant liquid storage
In tank (26), wherein the outlet end of the peristaltic pump (16) at the top of microorganism suspension holding vessel (25) passes through microorganism suspension conduit
(17) it is connect with No. three interfaces (28-3)；It is led by coagulant liquid the outlet end of peristaltic pump (16) at the top of coagulant liquid holding vessel (26)
Pipe (18) is connect with No.1 interface (28-1)；The automatically controlled end of vortex mixer (20) is movably arranged on top cover (28) by bracket, is mixed
After the stirring end of even device (20) passes through No. two interfaces (28-2), it is internal to be set to minim pipette (27)；The external packet of minim pipette (27)
Equipped with minim pipette insulating layer (22), the bottom end of the minim pipette insulating layer (22) and the back taper mouth (27-2) of minim pipette (27) bottom
It is level sensing area (27-1) that the outside in the level sensing area (27-1) is equipped with liquid level sensor (23) between outlet edge；Institute
It is connected with dropping liquid mouth (2) on the back taper mouth (27-2) stated, after dropping liquid mouth (2) passes through top cover (28), is set to cabin shell (1)
Inside, and each dropping liquid mouth (2) is correspondingly arranged in the upper end inlet of the internal No.1 collector (10) of cabin shell (1)；
The outer wall packet of the coagulant liquid holding vessel (26) is equipped with fluid reservoir insulating layer (14)；The fluid reservoir insulating layer (14) and
Silicon rubber heating sheet (30) and No. two temperature sensors (32) are embedded in minim pipette insulating layer (22)；The cabin shell
(1) inside is equipped with condenser (6), and the No.1 collector (10) is set to the inside of condenser (6)；Cabin shell (1)
Inner wall be fixed with No.1 temperature sensor (31)；The compressor (11), power supply adaptor (12) are respectively provided with from the bottom to top
In the inside of cabin shell (1), and it is located at condenser (6) external right side；The controller (13) is embedded in cabin shell (1)
Antetheca on；The air pump (7), air filter (5) are all set in the inside of cabin shell (1), and position from the bottom to top
Connect in the outlet end of condenser (6) outer left side, air pump (7) with air filter (5), air filter (5) with
Air flow meter (4) connection, air flow meter (4) are entrenched on the antetheca of cabin shell (1), and air flow meter connects on (4)
Have air conduit (3), the outlet side of air conduit (3) is connected with several air valves (21), each air valve by several branch pipes
(21) outlet side passes through pipeline and No. three interface (28-3) perforations connect；The coagulant liquid holding vessel (26) and No.1
The bottom of collector (10) is equipped on magnetic stirring apparatus (8), the power supply adaptor (12) and condenser (6), compressor
(11), controller (13), No.1 temperature sensor (31), No. two temperature sensors (32), silicon rubber heating sheet (30), liquid level pass
Sensor (23), peristaltic pump (16), magnetic stirring apparatus (8) and air pump (7) electrical connection；No.1 temperature sensor (31), No. two
Temperature sensor 33 and liquid level sensor (23) are connect with controller (13), controller (13) and compressor (11), magnetic force
Blender (8), vortex mixer (20), silicon rubber heating sheet (30), peristaltic pump (16) and air pump (7) connection.
2. a kind of microbial immobilized sphere preparation facilities according to claim 1, it is characterised in that: the No.1 is received
Storage (10) replaces with No. two collectors (9), is equipped with bracket (29) inside cabin shell (1), several No. two collectors (9) settings
It is split type connection between the transverse slat of bracket (29) and the stringer board of bracket (29) in the two sides up and down of the transverse slat of bracket (29).
3. a kind of microbial immobilized sphere preparation facilities according to claim 1, it is characterised in that: the top cover
(28) side on far from hinged end is screwed with buckle (19), card slot of buckle (19) the activity A Ka society in minim pipette (27) side wall
It is interior.
4. a kind of microbial immobilized sphere preparation facilities according to claim 1, it is characterised in that: outside the cabin
Hatch door (24) are hinged at the front openings of shell (1).
5. a kind of microbial immobilized sphere preparation facilities, it is characterised in that: its working principle: power supply adaptor (12) is packet
It includes and provides electric power with electric original copy including controller (13)；Controller (13) controls heat exchange by No.1 temperature sensor (31)
The compressor (11) of device, and make to maintain stable low temperature in cabin shell (1) by condenser (6)；
After dropping liquid mouth (2) are blocked, coagulant liquid directly prepares the minim pipette (27) of the respective insulating layer of disengaging and top cover (28) with
And in coagulant liquid holding vessel (26), certain temperature is cooled to after heating for dissolving, it is inserted into respective insulating layer, it is outstanding that microorganism is added
Supernatant liquid, top cover (28) after mixing, carry out subsequent dropping liquid, avoid gel residue when secondary transferring；
The condensation of minim pipette (27) inner gel cell mixture and coagulant liquid holding vessel (26) inner gel liquid in order to prevent, in container
Minim pipette insulating layer (22) and fluid reservoir insulating layer (14), minim pipette insulating layer (22) and fluid reservoir insulating layer are covered on outer wall
(14) there are No. two temperature sensors (32) and silicon rubber heating sheet (30) in, controller (13) passes through No. two temperature sensors (32)
It controls silicon rubber heating sheet (30), prevents gel cell mixture and coagulant liquid from cooling down and condense；
Top cover (28) are arranged at minim pipette (27) top；There are No. three interfaces (28-3) on top cover (28), simultaneously band is connect with air conduit (3)
There are air valve (21), when minim pipette (27) stops working, air valve (21) is remained turned-off, and there are No. two interfaces on top cover (28)
(28-2) and No.1 interface (28-1), connect with microorganism suspension conduit (17) and gel fluid catheter (18) respectively, and by compacted
Dynamic pump (16) in minim pipette (27), forms microorganism suspension holding vessel (25) and coagulant liquid holding vessel (26) interior liquid delivery
Gel cell mixture, the mixed temperature of the two can be lower than the initial temperature of gel, to guarantee the activity of microorganism；Control
Vortex mixer (20) in device (13) control top cover (28) mixes the gel cell mixture in minim pipette (27)；Microorganism
It is contacted below suspension holding vessel (25) with magnetic stirring apparatus (8), there is magneton to stir in tank to prevent microbial cell precipitates；
Controller (13) controls the positive and negative rotation of air pump (7) wind pressure rotor by the two-direction switch connecting with air pump (7) with reality
Now pump gas or pumping；In minim pipette (27) dropping liquid, air pump (7) pumps gas, air pump (7) one end and air filter (5) phase
Even the gel cell mixture to filter gas source impurity to prevent in pollution minim pipette, air filter (5) pass through air mass flow
Meter (4) is connected with air conduit (3), and air flow meter (4) is by adjusting air drainage amount to adjust in air conduit (3)
Air pressure, and then adjust the rate of minim pipette dropping liquid；When using individual minim pipettes (27), on corresponding air conduit (3)
Air valve (21) open, the corresponding air valve (21) of remaining minim pipette (27) is closed, to prevent air pressure from leaking；When multiple
Minim pipette (27) causes dropping liquid complete simultaneously in use, the how much different or drop velocities due to its inner gel mixed liquor are different
At time it is different, the corresponding air valve (21) of minim pipette (27) for completing dropping liquid is closed in time；
Minim pipette (27) lower part is back taper mouth (27-2), and back taper mouth (27-2) connects the dropping liquid mouth (2) of different pore size, dropping liquid
The aperture of mouth (2) determines the size of final immobilization sphere, has above dropping liquid mouth (2) installation site and does not cover minim pipette heat preservation
The region of layer (22), i.e., level sensing area (27-1), one end of the external liquid level sensor (23) installed issue laser light
Level sensing area (27-1) reaches the laser region of acceptance of liquid level sensor (23) other end, when the gel cell of colloidal and mixed
When closing the liquid level of liquid higher than level sensing area (27-1), due to Tyndall effect, laser is scattered, and reaches laser region of acceptance
Energy is lower；When the liquid level of the gel cell mixture of colloidal is lower than level sensing area (27-1), the permeability of laser compared with
Height, the energy for reaching laser region of acceptance is higher, at this point, liquid level sensor (23) sends signal, controller to controller (13)
(13) control peristaltic pump (16) conveying quantitative microorganism suspension and coagulant liquid into minim pipette (27), meanwhile, controller (13)
Control air pump (7) is become before pumping makes in minim pipette (27) in negative pressure to prevent gel cell mixture from not mixing from pump gas
With regard to drippage, after peristaltic pump (16) are infused a period of time, controller (13) starts vortex mixer (20) automatically, after mixing a period of time
Stop, when minim pipette (27) temperature reaches set temperature, air pump (7) is become pumping gas again from pumping, if necessary to open top
It covers (28) and microorganism suspension is then added, then dropping liquid mouth (2) need to be blocked in advance to prevent gel cell mixture from not mixing just
Drippage, when being mixed, silicon rubber heating sheet can be completed to heat gel cell mixture to it；Using magnetic stirring apparatus (8) with
Prevent the sphere stick to each other of incomplete gelation No.1 collector (10) Nei.
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| CN201811156048.6A CN108998372B (en) | 2018-09-30 | 2018-09-30 | Microorganism immobilized sphere preparation device |
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| CN201811156048.6A CN108998372B (en) | 2018-09-30 | 2018-09-30 | Microorganism immobilized sphere preparation device |
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| CN108998372B CN108998372B (en) | 2023-04-11 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109706072A (en) * | 2019-01-24 | 2019-05-03 | 王静 | A kind of microbe immobilized particles preparation facilities |
| CN111257080A (en) * | 2020-02-29 | 2020-06-09 | 右江民族医学院附属医院 | Assay sample processing apparatus for hepatobiliary surgery |
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| CN107236664A (en) * | 2017-08-08 | 2017-10-10 | 山东绿健生物技术有限公司 | A kind of immobilization device and its process for fixation for producing immobilised enzymes or cell |
| CN206646123U (en) * | 2017-04-06 | 2017-11-17 | 湖南三友环保科技股份有限公司 | The preparation facilities of immobilization material |
| CN108384773A (en) * | 2018-01-12 | 2018-08-10 | 天津农学院 | A kind of imbedded microbe gelled pill preparation facilities |
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| JPS6467189A (en) * | 1987-09-08 | 1989-03-13 | Yajima Mizuo | Immobilized gel and production thereof |
| CN203653543U (en) * | 2013-12-31 | 2014-06-18 | 上海海洋大学 | Production device of polyvinyl alcohol microorganism fixing granules |
| CN206646123U (en) * | 2017-04-06 | 2017-11-17 | 湖南三友环保科技股份有限公司 | The preparation facilities of immobilization material |
| CN107236664A (en) * | 2017-08-08 | 2017-10-10 | 山东绿健生物技术有限公司 | A kind of immobilization device and its process for fixation for producing immobilised enzymes or cell |
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| CN109706072A (en) * | 2019-01-24 | 2019-05-03 | 王静 | A kind of microbe immobilized particles preparation facilities |
| CN111257080A (en) * | 2020-02-29 | 2020-06-09 | 右江民族医学院附属医院 | Assay sample processing apparatus for hepatobiliary surgery |
| CN111257080B (en) * | 2020-02-29 | 2023-03-28 | 右江民族医学院附属医院 | Assay specimen processing device for hepatobiliary surgery |
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