CN208562389U - A kind of activity ventilation component and its active aeration type bioreactor and cell culture apparatus - Google Patents
A kind of activity ventilation component and its active aeration type bioreactor and cell culture apparatus Download PDFInfo
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- CN208562389U CN208562389U CN201820610454.4U CN201820610454U CN208562389U CN 208562389 U CN208562389 U CN 208562389U CN 201820610454 U CN201820610454 U CN 201820610454U CN 208562389 U CN208562389 U CN 208562389U
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Abstract
The utility model relates to a kind of activity ventilation component and its active aeration type bioreactors and cell culture apparatus, wherein the activity ventilation component includes the gas exchanger that internal gas flow channel is connected, peristaltic pump pump line and gas-guide tube three parts element, it is connected to form closed loop with the air inlet pipe and an air outlet pipe of bioreactor or cell culture apparatus by gas-guide tube, under the driving of peristaltic pump, the internal gas circulation of the bioreactor or cell culture apparatus flows to gas exchanger and realizes that the disperse of across micro-porous permeable bacteriological filtration film exchanges with extraneous gas under the action of partial pressure difference.When being placed in carbon dioxide incubator and three gas incubators in use, can not only save the gas regulating equipment of bioreactor and cell culture apparatus complex and expensive, but it is easy to operate.
Description
Technical field
The utility model relates to biofermentation field of cell culture, more particularly to a kind of activity ventilation component and its activity
Aeration type bioreactor and cell culture apparatus.
Background technique
One, cell culture apparatus and bioreactor: cell culture apparatus is the container of cell culture, such as common cell culture
Bottle, multi-layer board culture bottle, multi-layer cellular incubator-cell factory and cell culture bags (membrane material of permeable watertight) etc., by
In the lower (1-2ml/cm of liquid level2), without mixing the transmitting that can meet oxygen and nutriment, it is usually placed in incubator and adopts
State type culture is taken, it is easy to operate.So-called bioreactor refers to biological cell and its bioactive substance chemical reaction
Container, it usually needs the dynamic mixing of driving internal-response liquid stream come realize oxygen or with the transmitting of nutriment, such as agitator tank
Bioreactor, mantle bag bioreactor (airtight fluid-tight barrier film material) and airlift bioreactor etc. are outstanding
The bioreactor of floating culture formula.From the mode of ventilation and gas exchange, small-sized cell culture container and biological respinse
The gas exchanges of device are to realize container by the airspace between container cover and nozzle or the micro-porous permeable bacteriological filtration film covered
Inside and outside gas exchanges, and the cell culture bags of static gas wave refrigerator then realize bag by the gas dispersion of its own permeable watertight film
Inside and outside gas exchanges, this ventilating mode are the mode of the spontaneous ventilation under normal pressure；For fairly large cell culture apparatus (example
Such as 40 layers or more), fairly large stirring type bioreactor, wave mantle bioreactor and gas-lifting type biological respinse
Device, spontaneous ventilation are not able to satisfy its requirement to ventilation, then need active aeration type (or claiming active aeration type) fermentation and training
It supports, i.e., into oxygen-containing high live gas, the high metabolism gas of carbonated is held in discharge for positive drum pressure into incubator and reactor,
Wherein mantle bag bioreactor (as well known wave mantle bag bioreactor), which is removed, meets metabolism institute using activity ventilation
It needs outside the requirement of gas exchanges, it is also necessary to three-dimensional endoluminal in the positive pressure holding bag ventilated using activity, and gas-lifting type biological respinse
No matter device scale is required to active ventilation, mixing is above and below the rising driving a reaction liquid by blasting gas from bottom to realize
The transmitting of gas exchanges and nutriment.
Two, bio-incubators and its gas regulating: bio-incubator includes to be trained using the constant temperature of clean surrounding air
Case is supported, using the carbon dioxide incubator of clean surrounding air and a certain proportion of carbon dioxide gas, and utilizes cleaning
O2、CO2And N2Three gas incubator three types of a certain proportion of mixed gas.The interior environment of mammalian cell growth
In need to exist the carbon dioxide that centainly divides also in addition to needing oxygen to keep stable pH and meet certain work of cell
It is dynamic.Carbon dioxide in incubator needs to maintain between 2~10%, and usual 5% to keep the titanium dioxide dissolved in culture solution
The concentration of carbon.Since the gas concentration lwevel in air is very low, if cell is not cultivated in carbon dioxide incubator, culture solution
In HCO3It can be depleted, will affect the normal growth of cell in this way.So the culture of most zooblasts needs dioxy
Change carbon incubator or three gas incubators.Realize that accurate gas ratio is prepared, it is particularly significant to the growth for meeting different cells,
CO2And O2Sensor technology is mature, is commonly equipped with CO2And O2Two kinds of sensors, nitrogen=100%-CO2Concentration-O2Concentration.It is real
The monitoring and regulation of gas in existing incubator.For CO2For incubator, using the oxygen in environment pure air, do not need
Oxygen concentration is measured, CO is only measured and control2Concentration will be provided with CO in case2CO in sensor and controller detection case2
Concentration, and will test result and pass to the control control devices such as electric appliance and solenoid valve, when detecting CO in case2When concentration is relatively low,
Solenoid valve, CO will be automatically opened2Into in cabinet, until CO2Concentration reaches set concentration, and solenoid valve can just close, case
Interior CO2Cutting, reaches stable state.Gas mixing pump is by bottom CO in case2Gas mixes well with air, after uniform, again
It injects in case, avoids CO2Layering or non-uniform phenomenon.If controlling oxygen concentration, carbon dioxide incubator can not
Culture needs the environment of hyperoxia (being higher than 22%) or hypoxemia (lower than 20%), needs using three gas incubators, is exactly in tradition two
Oxygen and nitrogen are increased on the basis of carbonoxide incubator and configure special oxygen concentration probe.O is set in case2Gas is dense
Spend the oxygen sensor and solenoid valve controller of detection.Currently, carbon dioxide incubator and three gas incubators are current biology doctors
The equipment and CO that medicine field laboratory generally uses2And O2One of gas detection and the most accurate equipment of regulation.
The existing active ventilating mode of three: (such as multi-layer cellular incubator-is thin for existing activity aeration type cell culture apparatus
Born of the same parents factory) and bioreactor (such as stirred-tank reactor, mantle reactor, gas lift reactor) ventilating mode be usually will
The gas of external air source, the gas that the compressed gas or gas compressor for such as storing gas cylinder generate, is filtered, surveys
Determine and mix the ratio of gas with various ingredient, positive pressure inputs in biofermentation incubator or bioreactor, while will be inside it
Gas discharge is realized that existing activity ventilation is characterized in that full gas filters transient discharge formula, in order to prevent outlet mouth of pipe end
Germ contamination, be also equipped with sterilizing filter in outlet port.The shortcomings that existing activity aerating system, needs positive pressure
Gas source, inlet port and outlet port are required to setting dead-end filtration formula sterilizing filter, need oxygen O2And CO2Sensor and
The controller of gas mixer, pressure and flow is accurate to ensure various gas ratios, also needs on the filter of gas outlet sometimes
The problem of needing the blocking filter membrane when heater is arranged to prevent a large amount of band water vapor gas discharges in gas discharge outlet, it is therefore, existing
Bioreactor and cell culture apparatus active aerating system it is not only expensive but also complicated for operation, be especially unsuitable for small rule
The biofermentation of mould (incubator scale) and cell culture.In addition, lasting transient exclusion gas, also results in clean gas
Waste.
The conveying of the fluid of four, peristaltic pumps: peristaltic pump is also known as peristaltic pump, including peristaltic pump motor driver, peristaltic pump
Head and 3 part of peristaltic pump pump line, motor driver are the power section of peristaltic pump, and Peristaltic pump head has pipe clamp peristaltic pump pump line
Card slot, be provided with multiple (usual 2-3) rollers on the rotor by driver driving inside set, peristaltic pump pump line is high resiliency
The hose of anti-extrusion, when motor driver drives rotor rotation, roller repeatedly extrudes peristaltic pump pump line, to squeeze driving tube
Interior fluid (liquid or gas) is flowed to a direction, and biggest advantage is that non-intervention free of contamination gas or liquid are defeated
Give and all pump classes in, unique non-intervention free of contamination gas or liquid delivery pump, especially in biomedicine field by wide
General application.
Five, shaking tables: shaking table is to realize the commonly used equipment of liquid mixing in container by shaking and shaking, in biofermentation and
Field of cell culture, especially to small-sized biological reactor using very extensive, usually used shaking table shake and concussion driving biology
Inside reactor react culture solution non-intervention type mixing, thus realize nutriment transmitting and gas gas-liquid interface friendship
It changes.Shaking table is divided into plane convolution concussion shaking table according to the mode that it shakes, and the variety classes such as three-dimensional shaking table and seesaw shaking table can
According to the small suitable shaking table of the structure choice efficiency high shear force of different reactor.
The utility model problem to be solved: existing equipment, such as carbon dioxide incubator and three gas incubators are utilized
Accuracy controlling to gas ratio and the non-intervention free of contamination conveying using peristaltic pump to gas, it is logical to develop novel active
Device of air and novel active aeration type bioreactor and cell culture apparatus are logical with the activity for solving existing transient discharge formula
Gas bioreactor and cell culture apparatus need the problem of gas conditioning system and complex operations costly.
Utility model content
The shortcomings that the purpose of the utility model is to overcome the above-mentioned prior arts provides a kind of without individual complex and expensive
Gas filtration, CO2And O2Sensor and controller, gas mixer, pressure and flow control system are saved gas and are not needed
Gas Flowrate Control System, merely with existing incubator such as carbon dioxide incubator or three gas incubators to the automatic of gas
Activity ventilation component and its activity ventilation are developed in the non-intervention gas conveying of the mixing and peristaltic pump of regulation and shaking table driving
Formula bioreactor and cell culture apparatus.
To achieve the goals above, the activity ventilation component of the utility model and active aeration type bioreactor and thin
Born of the same parents' incubator uses following scheme:
The activity ventilation component includes at least three parts element that internal gas flow channel is connected:
Gas exchanger, the gas exchanger be with internal gas flow channel and serial communication in two pipeline section of gas-guide tube it
Between cavity, sealed set, which has, at least one wall surface of the cavity allows gas to penetrate to realize gas exchanges inside and outside cavity
But the micro-porous permeable bacteriological filtration film for stopping bacterium to penetrate；
Peristaltic pump pump line, the peristaltic pump pump line are Peristaltic pump head pipe clamp and the bullet for squeezing its internal gas flow of driving
Property hose section；
Gas-guide tube, the gas-guide tube is between the connection peristaltic pump pump line and the gas exchanger and the two is distinguished
Gas delivery conduit between the air inlet pipe and an air outlet pipe of stand-by bioreactor or cell culture apparatus.
The gas exchanger is to have internal gas flow channel made of the fusion of the micro-porous permeable bacteriological filtration film as described in two layers
Micro-porous permeable bacteriological filtration bag cavity, the both ends fusion of the micro-porous permeable bacteriological filtration bag has a connection ozzle, the connection ozzle with lead
Between tracheae by be closely connected or integral fusion connection.
The gas exchanger is to have internal gas flow channel made of the fusion of the micro-porous permeable bacteriological filtration film as described in two layers
Micro-porous permeable bacteriological filtration bag cavity, the micro-porous permeable bacteriological filtration bag one end fusion there are two be connected to ozzle, two connection ozzles
Between two layers of mantle merge to extend to form into bag and be connected to the U-shaped airflow channel that is connected of ozzles, the communicating pipe with two
Between mouth and gas-guide tube by be closely connected or integral fusion connection.
The gas exchanger is to be provided with two layers of barrier property mantle of the transom of ventilative bacteriological filtration film sealing to merge
The micro-porous permeable bacteriological filtration bag cavity with internal gas flow channel, micro-porous permeable bacteriological filtration bag both ends fusion has connection ozzle,
Between connection ozzle and the gas-guide tube by be closely connected or integral fusion connection.
The gas exchanger is to be provided with two layers of barrier property mantle of the transom of ventilative bacteriological filtration film sealing to merge
The micro-porous permeable bacteriological filtration bag cavity with internal gas flow channel, the micro-porous permeable bacteriological filtration bag one end fusion there are two connection
Ozzle, two two layers of mantles being connected between ozzles, which merge to extend to form into bag, is connected to the U-shaped gas that ozzles are connected with two
Circulation road, between connection ozzle and the gas-guide tube by be closely connected or integral fusion connection.
The gas exchanger has described micro- for sealing fusion in the braced frame of the hard material of both ends band connection ozzle
Hard skeleton cavity made of the ventilative bacteriological filtration film in hole, by being closely connected between connection ozzle and the gas-guide tube.
It is attached to band venthole net outside the micro-porous permeable bacteriological filtration film and plays support protection micro-porous permeable bacteriological filtration film
Mesh strip.
The gas-guide tube is connected to two or more gas exchangers by three-way connection or multiple-pass joint in parallel or series
To increase gas exchange efficiency, or it is connected to two or more bioreactor or cell culture apparatus in parallel or series to increase training
The scale of supporting.
Pipe of the gas-guide tube between gas exchanger and the bioreactor or the escape pipe of cell culture apparatus
The air pressure adjustment for adjusting pipe diameter size and adjusting the bioreactor or cell culture apparatus air pressure inside is provided in section
Device.
The active aeration type bioreactor configurations have the activity ventilation component；The bioreactor is
Wave bioreactor, airlift bioreactor, shaking flask bioreactor and stirring type bioreactor, the biology are anti-
Answer the air inlet pipe and an air outlet pipe that supplied gas disengaging is provided on device, the gas-guide tube and the biological respinse of the activity ventilation component
The air inlet pipe and an air outlet pipe of device is connected to form airflow channel closed loop.
The bioreactor is mantle bioreactor, and the mantle bioreactor is to be merged by 2 layers of mantle
Plane (2D) mantle bioreactor, or three-dimensional (3D) mantle bioreactor, described three-dimensional (3D) mantle bioreactor
Surface area at least 1 layer mantle in 2 layers of mantle in its fusion circle is greater than 5% or more the surrounded area of plane of fusion circle.
The air inlet pipe and an air outlet pipe perforation of the mantle bioreactor be blended in two layers of the mantle reactor it is soft
The fusion side of film, or perforation are blended on plane mantle, or perforation is blended in the mouthpiece cover that the nozzle of mantle bag is configured, institute
It states perforation and is blended in the air inlet pipe and an air outlet pipe of mouthpiece cover and be respectively arranged at different mouthpiece cover or same mouthpiece cover, be set to same
The air inlet pipe and an air outlet pipe of one mouthpiece cover extends into depth different in nozzle or in reactor.
The mantle bioreactor is to drive internal liquid formation wave to realize liquid mixing and gas by shaking
The wave mantle bioreactor of exchange.
The mantle bioreactor is to realize liquid mixing and gas exchanges and being aerated to its internal-response liquid
Gas-lifting type mantle bioreactor, the air inlet pipe of the gas-lifting type mantle bioreactor is provided with and communicated in bag to be extended to instead
The aeration tube for the solarization air cap for answering device bottom belt tiny.
The active aeration type cell culture apparatus is configured with the activity ventilation component, and the cell culture apparatus is
Rigid plastics cell culture container-cell factory of monolayer cell culture container-culture bottle or multilayer, the single-layer or multi-layer
Rigid plastics cell culture apparatus has one or more incubator nozzles, mouthpiece cover is configured on the incubator nozzle, in institute
State in mouthpiece cover perforation and be provided with air inlet pipe or/and escape pipe, the air inlet pipe and an air outlet pipe may be disposed at same mouthpiece cover or
It is respectively arranged at different mouthpiece covers, the air inlet pipe and an air outlet pipe for being set to same mouthpiece cover extends into nozzle or in reactor
Different depth, the gas-guide tube of the activity ventilation component is respectively with the air inlet pipe and an air outlet pipe of cell culture apparatus by being connected
Form the closed loop of airflow channel.
The meaning of the utility model: using the active aeration type bioreactor and cell culture apparatus of the utility model,
For circulating gas exchanges, without complicated pressure and flow control system and the mistake to all gas on airflow pipeline
It filters, the gas componant inside and outside cell culture apparatus or bioreactor, for example oxygen O2With carbon dioxide gas CO2Partial pressure and surrounding
Environmental gas realizes gas exchanges by the filter membrane partial pressure difference disperse of gas exchanger, saves gas, culture solution does not steam
Hair, does not block filter membrane, can use temperature, gas configuration ratio that incubator accurately controls, optimal humidity gaseous environment, no
Need the gas Flowrate Control System of individual valuableness.The activity ventilation component or gas exchanger of the utility model are placed in
CO2It is used in incubator or three gas incubators, utilizes gas ratio in the accurate regulating box of gas sensor and controller of incubator
Example realizes the active aeration type fermented and cultured of bioreactor and cell culture apparatus, had not only saved multiple equipment and gas but also operated letter
It is single.
Detailed description of the invention
Fig. 1 is the overall structure diagram of active aeration type bioreactor embodiment 1.
Fig. 2 is the structural schematic diagram of the activity ventilation component 1 of embodiment 1.
Fig. 3 is 1 structural schematic diagram of gas exchanger of embodiment 1.
Fig. 4 is 2 structural schematic diagram of gas exchanger of embodiment 1.
Fig. 5 is the structural schematic diagram of the gas exchanger 3 of embodiment 1.
Fig. 6 is the structural schematic diagram of the gas exchanger 4 of embodiment 1.
Fig. 7 is the structural schematic diagram of the gas exchanger 5 of embodiment 1.
Fig. 8 is the structural schematic diagram of the gas exchanger 6 of embodiment 1.
Fig. 9 is the structural schematic diagram of the gas exchanger 7 of embodiment 1.
Figure 10 is the structural schematic diagram of the activity ventilation component 2 of embodiment 1.
Figure 11 is the structural schematic diagram of the mouthpiece cover of the bioreactor of embodiment 1.
Figure 12 uses schematic diagram for active aeration type bioreactor embodiment 1.
Figure 13 is the overall structure diagram of active aeration type bioreactor embodiment 2.
Figure 14 uses schematic diagram for active aeration type bioreactor embodiment 2.
Figure 15 is the overall structure diagram of active aeration type bioreactor embodiment 3.
Figure 16 is the overall structure diagram of active aeration type bioreactor embodiment 4.
Figure 17 is the overall structure diagram of active aeration type bioreactor embodiment 5.
Figure 18 is the structural schematic diagram of active aeration type cell culture apparatus embodiment 1.
Figure 19 is the structural schematic diagram of active aeration type cell culture apparatus embodiment 2.
Figure 20 is outside carbon dioxide/tri- gas incubators of active aeration type bioreactor and cell culture apparatus using signal
Figure.
Appended drawing reference:
1 gas exchanger, 2 peristaltic pump pump line, 3 gas-guide tube, 4 barostat, 5 plane mantle wave bioreactor
(plane nozzle) 6 Peristaltic pump head 7 connects 8 support frame of ozzle, 9 10 mesh strip of micro-porous permeable bacteriological filtration film, 11 mouthpiece cover 12
18 rotor of air inlet pipe 13 escape pipe, 14 fixing clamp, 15 shaker platform, 16 shaking table, 17 peristaltic pump motor driver, 19 roller
20 plane mantle wave bioreactors (fusion side nozzle) 21 tee tube, 22 plane mantle gas lift bioreactor (flat tube
Mouthful) 23 liquid inlet/outlet pipe, 24 aeration tube 25,27 L shape branch of three-dimensional mantle wave reactor (plane nozzle) 26 stiff sheet shell support
28 culture bottle of frame, 29 multi-layer cellular incubator, 30 carbon dioxide/tri- gas incubators, 31 constant-temperature table 32 fusion circle, 33 filter
Mycoderm fusion is at the three-dimensional mantle wave bioreactor of 34 jointing 35 (nozzle when fusion)
Specific embodiment
In order to more clearly describe the technology contents of the utility model, carry out combined with specific embodiments below into one
The description of step.
Active aeration type bioreactor embodiment 1 (Fig. 1-12)
Shown in FIG. 1 is active 1 overall structure diagram of aeration type bioreactor embodiment, by activity ventilation component and
Bioreactor two parts composition.Activity ventilation component is made of 3 subelements that internal gas flow channel is connected, including gas
Exchanger 1, peristaltic pump pump line 2 and gas-guide tube 3.Gas exchanger 1 is with internal gas flow channel and serial communication is in gas-guide tube 3
Cavity between two pipeline sections allows gas to penetrate to realize in cavity by sealing fusion on the rigid support frame 8 of band connection ozzle 7
The outer gas exchanges but micro-porous permeable bacteriological filtration film 9 for stopping bacterium to penetrate forms, in flat box-like, both ends be provided with connection ozzle 7 with
Gas-guide tube 3 is fitted close connection.Peristaltic pump pump line 2 is 6 pipe clamp of Peristaltic pump head and the bullet for squeezing its internal gas flow of driving
Property hose section.For 2 pipe clamp of peristaltic pump pump line in the card slot of Peristaltic pump head 6, motor driver 17 drives rotor 18 to rotate, and turns
Roller 19 is provided on son 18, the rotation of rotor 18 drives roller 19 to repeatedly extrude peristaltic pump pump line 2, its internal gas is driven to pass through
Gas-guide tube 3 is to mantle reactor internal circulation flow.Gas-guide tube 3 be connection peristaltic pump pump line 2 and gas exchanger 1 between and two
Person's gas delivery conduit between the air inlet pipe 12 and escape pipe 13 of plane mantle wave bioreactor 5 respectively.Plane is soft
Film wave bioreactor 5, i.e., fusion reaction device nozzle forms by two layer plane mantle peripheral fusions and on the mantle of upper layer, week
Fusion circle 32 is formed at the fusion of side.Be threadedly engaged mouthpiece cover 11 on reactor nozzle, in mouthpiece cover 11 perforation be provided with into
Tracheae 12 or escape pipe 13.Air pressure tune in marsupial is set on the pipeline of the gas-guide tube 3 between gas exchanger 1 and escape pipe 13
Save device 4, by adjust gas-guide tube 3 bore adjust mantle bag holding heave state and air pressure inside, peristaltic pump pump line 2 with lead
It is connected between tracheae 3 by jointing 34.
In a kind of preferably embodiment, as shown in figure 3, gas exchanger 1 be the band of integrated thermoplastic shaping even
The 3-tier architecture that the support frame 8 of pressure inlet 7 and the fusion of micro-porous permeable bacteriological filtration film 9 of upper and lower surface sealed set are constituted, is in flat box-like
Shape.
In another preferably embodiment, as shown in figure 4, gas exchanger 1 be in the structure basis of Fig. 3, it is attached
Equipped with 5 layers of structure for supporting the upper and lower mesh strip 10 of protection micro-porous permeable bacteriological filtration film to constitute.
In another preferably embodiment, as shown in figure 5, the band that gas exchanger 1 is integrated thermoplastic shaping connects
The cylindric support frame 8 of pressure inlet 7, connection ozzle 7 are arranged on cylinder peripheral wall, and sealed set has micro-porous permeable bacteriological filtration on cylinder end face
Film 9 is simultaneously attached to the mesh strip 10 for supporting protection micro-porous permeable bacteriological filtration film outside micro-porous permeable bacteriological filtration film.
In another preferably embodiment, as shown in fig. 6, the band that gas exchanger 1 is integrated thermoplastic shaping connects
The cylindric support frame 8 of pressure inlet 7, connection ozzle 7 are arranged on cylinder end wall, and micro-porous permeable bacteriological filtration film 9 is provided on cylinder peripheral wall,
The mesh strip 10 for supporting protection micro-porous permeable bacteriological filtration film is attached to outside micro-porous permeable bacteriological filtration film 9.
In another preferably embodiment, as shown in fig. 7, gas exchanger 1 is the micro-porous permeable as described in two layers
Micro-porous permeable bacteriological filtration film bag with internal gas flow channel made of bacteriological filtration film 9 merges, micro-porous permeable bacteriological filtration film bag both ends
Fusion has connection ozzle 7.
In another preferably embodiment, as shown in figure 8, gas exchanger 1 is to be provided with ventilative bacteriological filtration film 9 to seal
Transom two layers of barrier property mantle fusion made of with internal gas flow channel micro-porous permeable bacteriological filtration bag cavity, obstructing
Property mantle transom merge place with ventilative bacteriological filtration film 9, form bacteriological filtration film and merge side 33, the micro-porous permeable bacteriological filtration bag both ends are melted
Conjunction has connection ozzle 7.
In another preferably embodiment, as shown in Fig. 9, gas exchanger 1 is to be provided with micro-porous permeable bacteriological filtration film
Micro-porous permeable bacteriological filtration bag cavity with internal gas flow channel made of two layers of barrier property mantle fusion of the transom of 9 sealings,
At the transom of barrier property mantle is merged with ventilative bacteriological filtration film 9, forms bacteriological filtration film and merge side 33, the micro-porous permeable bacteriological filtration bag
One end fusion merge and extend to form and two into bag there are two ozzle 7, two layers of mantle between two connection ozzles 7 are connected to
The U-shaped airflow channel that connection ozzle 7 is connected.
It is the activity ventilation component structure diagram of embodiment 1 as shown in Figure 10 in another preferably embodiment
2, it is integrated connection between peristaltic pump pump line 2 and gas-guide tube 3 with activity being a difference in that for component structure diagram 1 of ventilation,
That is peristaltic pump pump line 2 and gas-guide tube 3 elastic hose that is all peristaltic pump tubing matter, functionally defines peristaltic pump pump line and leads
The elastic hose section of the difference of tracheae, i.e. Peristaltic pump head institute pipe clamp is defined as peristaltic pump pump line, and is managed in Peristaltic pump head
All elastic hose other than card is then defined as gas-guide tube, rather than is to define from material on peristaltic pump pipe material-structure.It leads
Tracheae 3 is to act the pipe for being connected to airflow channel other than Peristaltic pump head pipe clamp and the pipeline section for squeezing driving internal gas flow
Road is still defined as gas-guide tube even if being peristaltic pump pump line in material.
Figure 11 show the structural representation of the mouthpiece cover 11 cooperated on the active aeration type bioreactor nozzle of embodiment 1
Scheme, perforation is provided with air inlet pipe 12 and escape pipe 13 in mouthpiece cover 11, and the two extends depth difference in mouthpiece cover.Certainly, exist
During others are implemented, air inlet pipe 12 and escape pipe 13 can also be separately positioned in two different mouthpiece covers.
Figure 12 show the use signal on shaking table of the active aeration type wave mantle bioreactor of embodiment 1
Figure is placed on the platform 15 of shaking table 16 in use, mantle reactor is fixed in fixed frame folder 14, peristaltic pump pump line 2 is blocked
Gu Peristaltic pump head 6 is installed in the wriggling pump controller of shaking table and in the card slot of Peristaltic pump head 6, shaker platform 15 is shaken
Dynamic drive makes internal media body form wave, thus realize gas and fluid exchange and mix, therefore also known as wave biological respinse
Device, under the driving of peristaltic pump, mantle inside reactor gas is recycled to gas exchanger 1 and under the action of partial pressure difference with outside
Portion's gas realizes the disperse exchange of across micro-porous permeable bacteriological filtration film 9, the O of the high keto sectional pressure outside gas exchanger 12Disperse enters gas friendship
In parallel operation 1, and the CO of internal high carbon dioxide partial pressure2It then permeates into outside gas exchanger 1.In gas exchanger 1 and escape pipe
The barostat 4 being arranged on gas-guide tube 3 between 13 can adjust the drum of mantle reactor by adjusting the bore of gas-guide tube 3
Play state and internal pressure.When be placed in carbon dioxide incubator or three gas incubators in use, using incubator gas
Regulator control system realizes that reactor to the accuracy controlling of oxygen and carbon dioxide gas, had not only saved the gas regulating equipment of reactor, but also
It is easy to operate.
The mode that shaking table 16 shakes according to it, is divided into planar tracks shaking table, three-dimensional shaking table and seesaw shaking table, in use,
The shaking table that can be suitble to according to the cell of culture, the structure of reactor, mixed effect and the small selection of shearing force.Microculture is usual
It is placed in common constant-temperature table, when carrying out animal cell culture, CO need to be placed in2Incubator or three gas incubators.
The needs of peristaltic pump pump line 2 are placed in the correct direction in 6 card slot of Peristaltic pump head, i.e., inside driving peristaltic pump pump line
Gas enters the direction of bioreactor or cell culture apparatus air inlet pipe, because opposite direction driving will lead to gas from gas exchanges
The ventilative bacteriological filtration membrane filtration of device goes out, so that influence gas exchange efficiency and mantle bioreactor heaves effect.
When needing largely with efficient gas exchanges, threeway or multiple-pass joint can be used can be by two by gas-guide tube 3
Or to increase gas exchange efficiency, when needing to increase culture scale, threeway can be used in multiple 1 serial or parallel connections of gas exchanger
Or multiple-pass joint passes through gas-guide tube 3 for two or more bioreactor serial or parallel connections to increase culture scale.
The material of peristaltic pump pump line 2 needs to have certain elasticity, and wearability bears the ability of pressure, and certain degree of hardness is airtight
The features such as property is good, and adsorptivity is low, temperature tolerance is good, non-aging, non-swelling, anticorrosive, precipitate is low.Commonly material includes:
Silicon rubber, fluorubber, Teflon, rubber, plastics, synthetic material etc..
The support frame of the skeleton of the band connection ozzle hard material of the present embodiment is formed by integrated thermoplastic shaping, can also be with
It is formed by two layers of stiff sheet frame with ozzle heat seal is connect.
The preferred hydrophobic micropore filter membrane of micro-porous permeable bacteriological filtration film 9,0.20 μm of aperture are used for aseptic filtration, such as poly-
The hydrophobic vent filter film of tetrafluoroethene PTFE has the characteristics such as fire-retardant permeable watertight, high temperature resistant, anti-strong acid-base, nontoxic, commonly uses
In the disperse of the cell culture apparatus of gas aseptic filtration and the small stationery culture of aeration type cell culture apparatus and bioreactor
The exchange of formula cross-film.
Gas exchanger is the cavity with internal gas flow channel and serial communication between two pipeline section of gas-guide tube, the chamber
There is sealed set at least one wall surface of body allows gas to penetrate to realize gas exchanges inside and outside cavity but bacterium is stopped to penetrate
Micro-porous permeable bacteriological filtration film.Gas exchanger is divided into soft-bag type and two kinds of rigid support frame, and soft-bag type gas exchanger can be
Micro-porous permeable bacteriological filtration film bag made of two layers of micro-porous permeable bacteriological filtration film merge with connection ozzle, can also for two layers barrier property mantle and
It is described to have transom every punching on property mantle bag every property mantle bag made of connection ozzle fusion, melt on the transom
Conjunction has micro-porous permeable bacteriological filtration film 9；Rigid support frame gas exchanger is that fusion is sealed on the rigid support frame of band connection ozzle
There is micro-porous permeable bacteriological filtration film to form, it can be in the cavity of the random geometries hard skeletons such as flat box-like or bobbin shape.
It is connected between the connection ozzle and gas-guide tube of the gas exchanger of micro-porous permeable bacteriological filtration film pocket type by being fitted close
Or integral fusion connection, the integral fusion connection as gas-guide tube directly merge connection with mantle bag.
The mantle bioreactor of the present embodiment is not provided with special liquid inlet/outlet pipe, and culture liquid material is by opening nozzle
Lid is injected and is removed by nozzle.When larger mantle bioreactor need to be arranged on mantle bag special liquid into
Outlet pipe is connected to setting liquid inlet/outlet pipe with air inlet pipe by 3 siphunculus.
In the present embodiment 1, the outer surface attachment of the micro-porous permeable bacteriological filtration film 9 of gas exchanger 1 is provided with to have and supports to protect
The mesh strip 10 with mesh of micro-porous permeable bacteriological filtration film is protected, micro-porous permeable bacteriological filtration film 9 can be fused in mesh strip 10 through ultrasound
Face.In others are implemented, it may also set up support outside the gas exchanger of micro-porous permeable bacteriological filtration film pocket type and protection be micro-
The mesh strip of the ventilative bacteriological filtration film in hole.
In a kind of preferably embodiment, leading between gas exchanger 1 and mantle bioreactor escape pipe 13
The barostat 4 that is arranged on tracheae 3 can adjust mantle reactor and heave state by adjusting the pipe diameter size of gas-guide tube 3
And internal pressure.
The gas exchanger may be in constant incubator/room in atmospheric environment carrying out the fermentation training of microorganism
It supports, but the culture of zooblast is then needed to be placed in gaseous environment provided by carbon dioxide incubator or three gas incubators
It uses.Gas inside and outside gas exchanger is real by the filter membrane gas dispersion of gas exchanger under the action of partial pressure difference
Existing gas exchanges, utilize gas ratio in the gas sensor and controller regulating box of carbon dioxide incubator or three gas incubators
Example, without complicated pressure and flow control system and filter.
Active aeration type bioreactor embodiment 2 (Figure 13-14)
Figure 13 is the overall structure diagram of the embodiment 2 of active aeration type bioreactor provided by the utility model,
With 1 structure of embodiment be a difference in that mantle bioreactor be nozzle be blended in mantle merge side infusion bag structure put down
Thin-skinned film wave bioreactor 20, the present embodiment have air inlet pipe 12 except mantle bioreactor merges perforation fusion on side, out
Tracheae 13 and liquid inlet/outlet pipe 23, other structures are the same as embodiment 1.
Figure 14 is the embodiment 2 of active aeration type bioreactor provided by the utility model using schematic diagram, infusion
The mantle bioreactor 20 of bag structure is fixed by fixing clamp 14, is placed in shaker platform 15 and is used by the inclination of L bracket 27,
Enter escape pipe to prevent culture solution, other usage modes mix and with embodiment 1, and by the liquid of wave mechanism in gas-liquid
What the gas exchanges mechanism in face was realized.
Advantage of this embodiment is that being formed newly using the widely applied infusion bag configuration activity ventilation component of medical field
The active aeration type mantle bioreactor of type.Infusion bag is commonly used medical supplies, has nontoxic no pyrogen,
Be sealed, ne-leakage, it is sterile the advantages that, with the utility model activity ventilation component combine, can be used for suspending culture.Certainly,
Also the mantle bag similar with infusion bag structure, such as transfusion bags and urinal can be used.It is similar using infusion bag or infusion bag structure
Mantle bag carries out cell culture, and maximum obstacle is active aerating system, ventilative using traditional steel cylinder discharge formula, needs gas
The equipment of the complex and expensives such as ratio pressure traffic filtering degerming, the utility model solve this problem in that.
Active aeration type bioreactor embodiment 3 (Figure 15)
Figure 15 show active 3 overall schematic of aeration type mantle bioreactor (gas lift bioreactor) embodiment,
The present embodiment is both plane mantle nozzle except the essentially identical place of structure of mantle bioreactor, is provided with air inlet in mouthpiece cover
Pipe 12, escape pipe 13 are a difference in that and are communicated with liquid inlet/outlet pipe 23 in air inlet pipe 12 by 3 siphunculus, exist in air inlet pipe 12
It is provided in bag and extends to the aeration tube 24 that bottom is covered with aerating pore, drive gas in peristaltic pump pump line to recycle by peristaltic pump
Aeration tube 24 is flowed to, microbubble is sprayed, microbubble rises, and cause fluid density to reduce, the high density gas supplement of two sides, because
This forms liquid and recycles mixing up and down and realize gas exchanges, therefore the mantle bioreactor of embodiment 3 is not need shaking table
The gas-lifting type mantle bioreactor for realizing that liquid recycles mixing and gas exchanges up and down is shaken in concussion.
Active aeration type mantle bioreactor embodiment 4 (Figure 16)
The bioreactor overall structure diagram of activity aeration type shown in Figure 16, the bioreactor of the present embodiment are three-dimensional
3D mantle bioreactor, so-called 3D mantle reactor are that two layers of mantle at least 1 layer of its surface area in fusion circle is greater than fusion
5% or more the area of plane that circle 32 is surrounded forms single side three-dimensional mantle bioreactor and two-sided three-dimensional mantle biological respinse
Device, the three-dimensional mantle wave bioreactor 25 of the present embodiment, upper layer mantle are plane mantle, there is with real perforation fusion on mantle
The identical reactor nozzle of example 1 and 3 is applied configured with mouthpiece cover 11, lower layer's mantle is three-dimensional mantle, to form through thermoplastic shaping
Hemispherical mantle cavity, three-dimensional mantle wave bioreactor 25 are combined with stiff sheet and pass through and the same mold thermoplastic shaping of three-dimensional mantle
3D stiff sheet shell support 26.The other activity ventilation components of the present embodiment and other structures are same as Example 1, due to the present embodiment
Inverted cone bottom or hemispherical shaking flask of three-dimensional mantle bioreactor (the single side three-dimensional mantle) structure similar to hard, therefore using flat
The face shaking table that circles round shakes mixing, compared with traditional shaking flask, has the advantages that efficiently simply aerating system and disposable.
Active aeration type three-dimensional mantle bioreactor embodiment 5 (Figure 17)
Figure 17 is the structural schematic diagram of active aeration type bioreactor embodiment 5, the bioreactor two of the present embodiment
Layer mantle has the two-sided three-dimensional mantle wave bioreactor 35 that the three-dimensional mantle of thermoplastic shaping is constituted, i.e., the face of two layers mantle
Product is all larger than fusion and encloses the surrounded area of plane.The air inlet pipe 12 and escape pipe 13 and liquid inlet/outlet pipe of activity ventilation component
23 merge on fusion circle 32, and two-sided three-dimensional mantle wave bioreactor 25 is combined with stiff sheet through same with three-dimensional mantle below
The 3D stiff sheet shell support 26 of mold thermoplastic shaping, 3D stiff sheet shell support 26, which is located in L bracket, makes two-sided three-dimensional mantle wave biology
Reactor 25, which inclines to setting, returns back out tracheae 13 on shaker platform with liquidproof filling.The present embodiment removes the structure of mantle bioreactor
Different from embodiment 1 outer, the gentle body circulation exchange principle of structure of activity ventilation component is identical with embodiment 1.2,4 and 5.
In other active aeration type bioreactors are implemented, activity ventilation component can be from other different bioreactors
Combination, bioreactor can be shaking flask bioreactor, including conical flask and cone bottom bottle, be also possible to agitator tank, including magnetic
The agitator tank of power drive and mechanically operated agitator tank.
Active aeration type cell culture apparatus embodiment 1 (shown in Figure 18):
Figure 18 show active 1 overall schematic of aeration type cell culture apparatus embodiment, and cell culture apparatus is cell monolayer
Culture bottle 28 is configured with mouthpiece cover 11 on the nozzle of monolayer cell culture bottle 28, and perforation is provided with air inlet pipe 12 in mouthpiece cover 11
On a different plane with the height of escape pipe 13,12 and escape pipe 13 in mouthpiece cover.What monolayer cell culture bottle 28 configured
Activity ventilation component with above-mentioned activity aeration type bioreactor 1-5 configured activity ventilate component it is identical, active ventilation group
In the card slot of Peristaltic pump head 6, Peristaltic pump head 6 is assemblied in wriggling pump controller the peristaltic pump pipe clamp of part.In gas
It is provided with barostat 4 on the pipeline section of gas-guide tube 3 between exchanger 1 and the escape pipe 13 of Tissue Culture Flask 28, passes through gas
The caliber size for pressing adjuster 4 to adjust gas-guide tube 3 is adjusted by adjusting the air pressure in Tissue Culture Flask 28, so that high pressure be caused to train
Support environment.The active aeration type monolayer cell culture bottle of the present embodiment is mainly used for the cell culture model for hyperbaric environment
Research, such as simulation intraocular hypertension is to intraocular cell, influence of the hypertension to vascular endothelial cell and high cranium pressure to nerve cell
Research.
Active aeration type cell culture apparatus embodiment 2 (Figure 19):
2 overall schematic of Figure 19 activity aeration type cell culture apparatus embodiment, the cell culture apparatus of the present embodiment are multilayer
Cell culture apparatus, such as cell factory, the structure of the activity ventilation component of the present embodiment and active aeration type bioreactor are real
It applies a 1-5 and active aeration type cell culture apparatus embodiment 1 is identical, the air inlet of the present embodiment multi-layer cellular incubator
Pipe 12 and escape pipe 13 are separately positioned in 2 mouthpiece covers of multi-layer cellular incubator and are connected to formation with gas-guide tube 3 respectively and close
Cyclization road.The active aeration type multi-layer cellular incubator of the present embodiment is mainly used for large-scale cell culture and biotechnology produces
The production of product is supported compared with traditional active aeration type multi-layer cellular incubator using carbon dioxide incubator or three air cultures
The operation of gas filtration, the equipment of proportion adjustment flow pressure control system valuableness and complexity is saved in case culture.
Active aeration type bioreactor and cell culture apparatus CO2Embodiment 8 (Figure 20) is used outside/tri- gas incubators:
The bioreactor of activity aeration type shown in Figure 20 and cell culture apparatus CO2Whole signal is used outside/tri- gas incubators
Figure, when cell culture apparatus or bioreactor volume excessive (when being greater than incubator receiving volume) or excessively more than CO2/ tri- air cultures
When supporting the carrying capacity of case or when the excessive heat production of volume and power of peristaltic pump or/and shaking table influences culture temperature control, work as use
Bioreactor and cell culture apparatus volume it is excessive, shaking table and wriggling pump volume or the excessive influence carbon dioxide of heat production and three
The temperature control of gas incubator should not be placed in when the wet environment of carbon dioxide and three gas incubators, can take following 4 kinds not
Same settling mode: 1) bioreactor or cell culture apparatus of activity ventilation component and its connection is placed in carbon dioxide training
It supports in case or three gas incubators；2) passing through chamber through-hole/pilot hole or incubator door sealing gap by gas-guide tube will wriggle
Pump (including pump line, pump head and motor driver) is placed in outside carbon dioxide incubator or three gas incubators, remaining passes through gas-guide tube
It is placed in incubator；3) by the gas exchanger of activity ventilation component and peristaltic pump pump line, (including Peristaltic pump head and motor are driven
Dynamic device) it is placed in carbon dioxide incubator or three gas incubators, and by stand-by cell culture apparatus or bioreactor by leading
Tracheae is placed in outside carbon dioxide incubator or three gas incubators across the 30 door sealing gap of incubator and uses；4) only gas is handed over
Parallel operation 1 is placed in carbon dioxide incubator or three gas incubators, remaining equipment is placed in CO2Thermostatic chamber outside/tri- gas incubators makes
With, it will be including macro-organism reactor 25 shown in Figure 20, multi-layer cellular incubator 29, large-scale peristaltic pump 17 is with multichannel wriggling
It pumps pump head, and large-scale constant-temperature table 31 etc. and carbon dioxide culture is placed in across incubator door sealing gap by gas-guide tube
Schematic diagram is used outside case or three gas incubators, large-scale common thermostatic chamber is realized and large-scale constant incubator can be by small-sized
CO2The breakthrough of/tri- gas incubator culture zooblasts.
Connect and used in parallel: gas-guide tube goes out multiple gas-guide tubes for described two by three-way connection or multiple-pass joint branch
It is connected by way of in parallel or series between a or multiple gas exchangers to increase gas exchange surface product or by two
Or above cell culture apparatus or bioreactor into and out of tracheae serial or parallel connection be connected to increase culture scale.
Peristaltic pump: peristaltic pump is the widely applied fluid of biomedicine field (liquids and gases) conveying device, maximum excellent
Point is without the pollution-free tube fluid of intervention.Peristaltic pump includes driver, pump head and pump line three parts, and wherein driver is usually electricity
The rotor of machine driving, Peristaltic pump head include the pump case of pipe clamp peristaltic pump pump line and the roller for squeezing pump line, and pump line is by compacted
Dynamic pump pump head pipe clamp simultaneously drives the elastomeric hose section of its fluid flow inside.
The air inlet pipe and an air outlet pipe and liquid inlet/outlet pipe of mantle bioreactor, which usually penetrate through, is blended in mantle fusion side,
Perhaps it is anti-to be blended in 3 kinds of modes and mantle in mouthpiece cover joined together by the nozzle of plane mantle for upper layer plane mantle or perforation
Device is answered to be connected to.
Nozzle be blended in fusion while be in infusion bag structure fusion while nozzle, nozzle perforation be blended in it is epipial be film
Facial canal mouth
The utility model provides a kind of activity ventilation culture formula cell culture apparatus, and the cell culture apparatus configuration is
The activity ventilation component being applied in cell culture apparatus and/or bioreactor stated described is applied to cell culture apparatus
And/or the gas exchange systems in bioreactor.
The activity ventilation component of the utility model enumerates wave bioreactor, gas lift bioreactor except application domain is above-mentioned
Outside cell culture apparatus, it is also applied for shaking flask bioreactor, stirring type bioreactor, by the active ventilation group of utility model
Part is configured at existing shaking flask bioreactor, and stirring type bioreactor forms activity ventilation shaking flask bioreactor and stirring
Formula bioreactor, this specification not enumerate one by one by picture, but the structure and working principle and implementation of itself and activity ventilation component
The embodiment enumerated is identical.
In a kind of preferably embodiment, the cell culture apparatus is Tissue Culture Flask, the training of single-layer or multi-layer cell
Support device.
The utility model provides a kind of bioreactor, and the bioreactor configurations, which have, described is applied to cell
Activity ventilation component and applied in cell culture apparatus and/or bioreactor in incubator and/or bioreactor
Active aeration cycle gas exchange systems, circulating gas exchange systems are series at aeration type bioreactor or cell culture apparatus
Air inlet pipe and an air outlet pipe between form closed loop, can under the driving of peristaltic pump internal gas circulation, will this be practical new
The circulating gas exchanger of type is placed in CO2In incubator or three gas incubators, gas sensor and control using incubator
Gas ratio in device regulating box, only inside and outside biofermentation incubator or bioreactor the different gas componant of partial pressure difference across gas
Body exchanger filter membrane disperse exchange, realizes the aeration type fermented and cultured of cell culture apparatus or bioreactor, had both saved multiple equipment
It is again easy to operate with gas.
Gas sensor is the probe for monitoring gas concentration, concentration including source gas ingredient and enters cell culture apparatus
Or the concentration of the gas componant in bioreactor and in incubator, controller are that control enters cell culture apparatus or biology is anti-
Answer in device and in incubator gas flow valve.Existing aeration type cell culture apparatus or bioreactor needs are especially matched
Pneumatic filter, sensor and controller are set, the circulating gas exchanger of the active ventilating component of the utility model is placed in life
It is used in the widely applied incubator in object laboratory, it is special with aeration type cell culture apparatus or bioreactor not only to save these
The expensive device of configuration, and save these cumbersome operation sequences.
In this description, the utility model is described with reference to its specific embodiment.But it is clear that still can be with
The spirit and scope that various modification can be adapted and converts without departing from the utility model.Therefore, the description and the appended drawings should be considered as
It is illustrative and not restrictive.
Claims (15)
1. a kind of activity ventilation component, which is characterized in that the activity ventilation component includes that internal gas flow channel is connected
At least three parts element:
Gas exchanger, the gas exchanger is with internal gas flow channel and serial communication is between two pipeline section of gas-guide tube
Cavity, sealed set, which has, at least one wall surface of the cavity allows gas to penetrate to realize gas exchanges but resistance inside and outside cavity
Keep off the micro-porous permeable bacteriological filtration film that bacterium penetrates；
Peristaltic pump pump line, the peristaltic pump pump line are that Peristaltic pump head pipe clamp and extruding drive the elasticity of its internal gas flow soft
Pipe pipeline section；
Gas-guide tube, the gas-guide tube be connected between the peristaltic pump pump line and the gas exchanger and the two respectively with to
Gas delivery conduit between bioreactor or the air inlet pipe and an air outlet pipe of cell culture apparatus.
2. activity ventilation component according to claim 1, which is characterized in that the gas exchanger is as described in two layers
Micro-porous permeable bacteriological filtration bag cavity with internal gas flow channel made of the fusion of micro-porous permeable bacteriological filtration film, the micro-porous permeable bacteriological filtration
Bag both ends fusion have connection ozzle, between connection ozzle and the gas-guide tube by be closely connected or integral fusion company
It connects.
3. activity ventilation component according to claim 1, which is characterized in that the gas exchanger is as described in two layers
Micro-porous permeable bacteriological filtration bag cavity with internal gas flow channel made of the fusion of micro-porous permeable bacteriological filtration film, the micro-porous permeable bacteriological filtration
There are two ozzle is connected to, two layers of mantle between two connection ozzles is merged into bag to be extended to form and two for one end fusion of bag
The U-shaped airflow channel that is connected of connection ozzle passes through between connection ozzle and the gas-guide tube and is closely connected or integrated
Fusion connection.
4. activity ventilation component according to claim 1, which is characterized in that the gas exchanger is to be provided with ventilative filter
Micro-porous permeable bacteriological filtration bag chamber with internal gas flow channel made of two layers of barrier property mantle fusion of the transom of mycoderm sealing
Body, the micro-porous permeable bacteriological filtration bag both ends fusion have connection ozzle, pass through tight fit between connection ozzle and the gas-guide tube
Connection or integral fusion connection.
5. activity ventilation component according to claim 1, which is characterized in that the gas exchanger is to be provided with ventilative filter
Micro-porous permeable bacteriological filtration bag chamber with internal gas flow channel made of two layers of barrier property mantle fusion of the transom of mycoderm sealing
Body, one end fusion of the micro-porous permeable bacteriological filtration bag is there are two ozzle is connected to, and two layers of mantle between two connection ozzles are to bag
Interior fusion, which is extended to form, is connected to the U-shaped airflow channel that ozzle is connected with two, passes through between connection ozzle and the gas-guide tube
It is closely connected or integral fusion connects.
6. activity ventilation component according to claim 1, which is characterized in that the gas exchanger is both ends band connection pipe
Sealing fusion has hard skeleton cavity made of the micro-porous permeable bacteriological filtration film, the company in the braced frame of the hard material of mouth
By being closely connected between pressure inlet and gas-guide tube.
The component 7. activity according to any one of claim 2 to 6 is ventilated, which is characterized in that the micro-porous permeable bacteriological filtration
The mesh strip for serving with venthole net and supporting protection micro-porous permeable bacteriological filtration film is attached to outside film.
8. activity ventilation component according to claim 1, which is characterized in that the gas-guide tube passes through three-way connection or more
Pass joint is connected to two or more gas exchangers in parallel or series to increase gas exchange efficiency, or is connected to two in parallel or series
A or above bioreactor or cell culture apparatus is to increase culture scale.
9. activity ventilation component according to claim 1, which is characterized in that the gas-guide tube is in gas exchanger and institute
It states to be provided on the pipeline section between bioreactor or the escape pipe of cell culture apparatus and adjusts pipe diameter size and adjust described
The barostat of bioreactor or cell culture apparatus air pressure inside.
10. a kind of activity aeration type bioreactor, which is characterized in that the active aeration type bioreactor configurations are had the right
Benefit require any one of 1 to 9 described in activity ventilation component；The bioreactor is wave bioreactor, gas lift
Formula bioreactor, shaking flask bioreactor or stirring type bioreactor, be provided on the bioreactor supplied gas into
Air inlet pipe and an air outlet pipe out, the air inlet pipe and an air outlet pipe phase of the gas-guide tube and the bioreactor of the activity ventilation component
Connection forms airflow channel closed loop.
11. activity aeration type bioreactor according to claim 10, which is characterized in that the bioreactor is soft
Membrane bioreactor, the mantle bioreactor are plane mantle bioreactor made of being merged as 2 layers of mantle, or three-dimensional
Mantle bioreactor, the three-dimensional mantle bioreactor merge the surface of at least 1 layer mantle in 2 layers of mantle in circle for it
Product is greater than fusion and encloses 5% or more the surrounded area of plane.
12. activity aeration type bioreactor according to claim 11, which is characterized in that the mantle biological respinse
The air inlet pipe and an air outlet pipe perforation of device is blended in the fusion side of two layers of mantle of the mantle bioreactor, or perforation is blended in
On plane mantle, or perforation is blended in the mouthpiece cover that the nozzle of mantle bag is configured, it is described penetrate through be blended in mouthpiece cover into
Tracheae and escape pipe are respectively arranged at different mouthpiece cover or same mouthpiece cover, are set to the air inlet pipe and outlet of same mouthpiece cover
Pipe extends into depth different in nozzle or in reactor.
13. activity aeration type bioreactor according to claim 12, which is characterized in that the mantle bioreactor
To drive internal liquid to form the wave mantle bioreactor that wave realizes liquid mixing and gas exchanges by shaking.
14. activity aeration type bioreactor according to claim 12, which is characterized in that the mantle bioreactor
For the gas-lifting type mantle bioreactor for realizing liquid mixing and gas exchanges and being aerated to its internal-response liquid, the gas
The air inlet pipe of lift-type mantle bioreactor is provided with and communicated with the exposure for extending to the tiny solarization air cap of reactor bottom band in bag
Tracheae.
15. a kind of cell culture apparatus, which is characterized in that the cell culture apparatus is configured with any one of claims 1 to 9 institute
The activity ventilation component stated, the cell culture apparatus are thin for the rigid plastics of monolayer cell culture container-culture bottle or multilayer
Born of the same parents' culture vessel, the rigid plastics cell culture apparatus of the single-layer or multi-layer have one or more incubator nozzles, the training
Support and be configured with mouthpiece cover on device nozzle, perforation is provided with air inlet pipe or/and escape pipe in the mouthpiece cover, the air inlet pipe and
Escape pipe may be disposed at same mouthpiece cover or be respectively arranged at different mouthpiece covers, is set to the air inlet pipe of same mouthpiece cover and goes out
Tracheae extends into depth different in nozzle or in reactor, the gas-guide tube of the activity ventilation component respectively with cell culture apparatus
Air inlet pipe and an air outlet pipe by being connected to form the closed loop of airflow channel.
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| CN201820610454.4U CN208562389U (en) | 2018-04-26 | 2018-04-26 | A kind of activity ventilation component and its active aeration type bioreactor and cell culture apparatus |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108315256A (en) * | 2018-04-26 | 2018-07-24 | 上海久博生物工程有限公司 | Activity ventilation component and its active aeration type bioreactor and cell culture apparatus |
| CN116814432A (en) * | 2023-08-29 | 2023-09-29 | 苏州赛普生物科技股份有限公司 | Cell closed culture device and method with high air permeability |
-
2018
- 2018-04-26 CN CN201820610454.4U patent/CN208562389U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108315256A (en) * | 2018-04-26 | 2018-07-24 | 上海久博生物工程有限公司 | Activity ventilation component and its active aeration type bioreactor and cell culture apparatus |
| WO2019206207A1 (en) * | 2018-04-26 | 2019-10-31 | 上海久博生物工程有限公司 | Active ventilation assembly, active-ventilation-type bioreactor and cell culture device |
| CN108315256B (en) * | 2018-04-26 | 2024-04-09 | 上海久博生物工程有限公司 | Active aeration component, active aeration bioreactor and cell culture device |
| CN116814432A (en) * | 2023-08-29 | 2023-09-29 | 苏州赛普生物科技股份有限公司 | Cell closed culture device and method with high air permeability |
| CN116814432B (en) * | 2023-08-29 | 2023-11-24 | 苏州赛普生物科技股份有限公司 | Cell closed culture device and method with high air permeability |
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