CN101486967B - Culture fluid gas/liquid and liquid/liquid exchanger for tissue engineering tissue bionic cultivation - Google Patents
Culture fluid gas/liquid and liquid/liquid exchanger for tissue engineering tissue bionic cultivation Download PDFInfo
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- CN101486967B CN101486967B CN2009101031824A CN200910103182A CN101486967B CN 101486967 B CN101486967 B CN 101486967B CN 2009101031824 A CN2009101031824 A CN 2009101031824A CN 200910103182 A CN200910103182 A CN 200910103182A CN 101486967 B CN101486967 B CN 101486967B
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Abstract
The invention discloses a culture fluid gas-to-fluid and fluid-to-fluid exchanger that is used for biomimetic tissue cultivation in tissue engineering, an inlet and an outlet of a circular culture fluid are arranged in the chamber of an exchanger shell, an inlet and an outlet of a fresh culture fluid are arranged on the shell, an inlet and an outlet that are used for the fresh culture fluid to pass through and for communicating a hydrophilic hollow fiber semi-permeable biomembrane bunch that implements fluid-to-fluid exchange of the circular culture fluid with the fresh culture fluid are arranged in the chamber, a gas outlet and a gas inlet are arranged on the shell, an inlet and an outlet that are used for gas to pass through and for communicating a hydrophobic hollow fiber semi-permeable biomembrane bunch that implements gas-to-fluid exchange of the circular culture fluid with the gas are arranged inside the chamber, and an airtight space is formed inside the chamber of the shell. Oxygen, carbon dioxide, the fresh culture fluid and the circular culture fluid can finish the gas-to-fluid and the fluid-to-fluid exchange and update inside the exchanger without opening the chamber, and the chamber is closed so as to ensure the pulse-typed circulation of the fresh culture fluid and the circular culture fluid and to support the biomimetic tissue cultivation in tissue engineering.
Description
Technical field
The present invention relates to biomedical engineering tissue cultivating field, particularly a kind of nutrient solution gas/liquid of tissue engineering tissue bionic cultivation and liquid/liquid interchanger.
Background technology
The bioengineered tissue cultivation need be placed on the tissue of being cultivated in the nutrient solution in the culturing room of engineering tissue culture system and cultivate, and provides required nutrition by nutrient solution for the tissue of cultivating.Because nutrient solution uses after for some time nutritive substance wherein to consume, therefore need upgrading to the nutrient solution in the culturing room, fresh medium can be brought into nutritive substance in the culturing room, allow the nutrient solution in the culturing room can keep enough nutritive ingredients, for the tissue of cultivating provides nutrition.Nutrient solution when common existing engineering tissue culture system uses is changed, be to adopt the replacing fresh medium that opens a position with culturing room, promptly open culturing room and take out the used nutrient solution of part, fresh medium reinjects, this opening a position changed the mode of nutrient solution, in each process of changing nutrient solution, destroy the stable state of culturing process biological chemistry microenvironment easily, and, influence the effect of tissue culture with the active factor of the nutrient solution lost part tissue secretion that takes out; And this replacing nutrient solution complex operation that opens a position, cause bacterial contamination easily, cause the tissue culture failure.Publication number is CN1155690C's the technical scheme of " circulation and physiological stress simulating engineering tissue three-dimensional cultivation device ", the culture apparatus that provides a kind of continous pouring that nutrient solution is circulated, it is by being provided with circulation nutrient solution storage bottle, circulation nutrient solution storage bottle is communicated with the formation circulation by silicone tube with culturing room, the new training liquid import of circulation nutrient solution storage bottle is communicated with the fresh medium storage bottle by a silicone tube, culturing room is communicated with the waste collection bottle by silicone tube, make the circulation nutrient solution in the circulation nutrient solution storage bottle under the driving of liquid stream drive unit, form circulation and discharging through culturing room, and make the new training liquid in the fresh medium storage bottle under the driving of liquid stream drive unit, replenish fresh medium to circulation nutrient solution storage bottle, this culture apparatus can make the circulation nutrient solution form cycling stream through culturing room, avoid culturing room in cultivating process, repeatedly to open a position and more renewed training liquid, compare progressive to some extent with traditional culture apparatus, but there is not loop structure between this fresh medium storage bottle and the circulation nutrient solution storage bottle, fresh medium just adds injection circulation nutrient solution storage bottle to be mixed with the circulation nutrient solution, and in circulation nutrient solution storage bottle, inject and add a certain amount of fresh medium, just must discharge a certain amount of circulation nutrient solution, can cause the fresh culture liquid measure in the fresh medium storage bottle fewer and feweri like this, until using up, can only satisfy the nutrient solution consumption of short period, cultivate for the long period tissue engineering tissue, also need uncork to add fresh medium, though reduced the number of times of uncork interpolation fresh medium, compare with traditional culture apparatus progressive to some extent, the possibility of still exist polluting; And this device is just finished the replacing of the amount of circulation nutrient solution and fresh medium, can not form the exchange of the nutritive ingredient of circulation nutrient solution and fresh medium, and the active factor of tissue secretion meeting partial loss along with the discharging of nutrient solution equally in the tissue culture procedures can not utilize again.Simultaneously, the circulation nutrient solution storage bottle of this device is a cavity structure, the upper end of circulation nutrient solution storage bottle is provided with venting port and communicates with ambient atmosphere, a silicone tube that is communicated with gas bomb inserts from the import of circulation nutrient solution storage bottle upper end, in the nutrient solution in the micropore air injector insertion circulation nutrient solution storage bottle that the silicone tube end is provided with, and fresh medium storage bottle upper end is provided with venting port equally and communicates with ambient atmosphere, cause in the circulation nutrient solution storage bottle, all do not form enclosed space in the fresh medium storage bottle, so the circulation of the nutrient solution that circulates drives the only suitable filling type circulation of adopting a peristaltic pump to flow drive unit as liquid, as adopt the pulsed expansion pump that the nutrient solution in the circulation nutrient solution storage bottle will be ejected from venting port under the driving pressure of expansion pump, therefore the bionical circulation of pulsed of simulation heart movement can't be formed, the tissue engineering tissue bionic cultivation of real meaning can not be constituted.In view of above-mentioned reason, there is not the device of simulated lung visceral-qi body function of exchange, renal excretion metabolic waste function and human nutrition functions of physical supply simultaneously at present in the tissue engineering tissue cultivation field, the exchange of substance that can't realize real meaning is bionical, therefore need a kind of neither opening a position just can upgrade nutrient solution, can realize the device of the renewal of nutrient solution nutritive ingredient and gaseous constituent again, realize the bionic cultivation of tissue engineering tissue.
And the mode of gas-liquid exchange mainly contains in the tissue engineering tissue cultivation field at present: culturing bottle upper end blow-by, and allow air contact with the nutrient solution liquid level, form air and nutrient solution and exchange naturally; In culturing bottle, insert oxygen hose, at the oxygen pipe end micropore air injector is set, contacts oxygenation by inject oxygen generation bubble in nutrient solution with nutrient solution, the shortcoming of this mode is that bubble can cause liquid level to produce a large amount of foams, breed bacteria forms and pollutes easily, causes the tissue culture failure; Also have a kind of employing semi-permeable membranes with the sealing of culturing room mouth, nutrient solution is full of culturing room, realize the gas-liquid exchange by semi-permeable membranes, causes the chance polluted though reduced the gas-liquid clearing house, and exchange efficiency is low, incompatibility tissue engineering tissue suitability for industrialized production.
Summary of the invention
The objective of the invention is at the deficiencies in the prior art, a kind of nutrient solution gas/liquid and liquid/liquid interchanger of tissue engineering tissue bionic cultivation are provided, it by in the chamber that is arranged on housing be used for gas through and carry out the biological semi-permeable membranes boundling of hydrophobic hollow fiber of gas/liquid exchange with the circulation nutrient solution and be used for fresh medium through and the biological semi-permeable membranes boundling of hydrophilic hollow fibre that carries out liquid/liquid exchange with the circulation nutrient solution, need not open a position and just can make oxygen and carbon dioxide, fresh medium, the circulation nutrient solution finishes gas/liquid in interchanger and liquid/liquid exchange is upgraded, and by airtight chamber's assurance fresh medium, the pulsed circulation of circulation nutrient solution is for the bionic cultivation of realizing tissue engineering tissue provides support.
The object of the present invention is achieved like this: the housing that comprises belt chamber, the chamber of housing is provided with and is used for the circulation nutrient solution entrance and exit that links to each other with culturing room, be provided for the fresh medium entrance and exit that links to each other with the fresh medium holder on the housing, be provided in the chamber fresh medium through and be communicated with the inlet of fresh medium with the biological semi-permeable membranes boundling of hydrophilic hollow fibre that the circulation nutrient solution carries out the exchange of liquid/liquid, outlet, pneumatic outlet and the gas inlet that is used for linking to each other with source of the gas are set on the housing, be provided in the chamber gas through and be communicated with the inlet of gas with the biological semi-permeable membranes boundling of hydrophobic hollow fiber that the circulation nutrient solution carries out gas/liquid exchange, outlet makes the chamber of housing form enclosed space.
Owing to adopted such scheme, the chamber of housing is provided with and is used for the circulation nutrient solution entrance and exit that links to each other with culturing room, make this chamber form circulation passage, the circulation nutrient solution can be circulated between this interchanger and culturing room, for the cultivation tissue in the culturing room provides nutrition with culturing room.On housing, be provided for the fresh medium entrance and exit that links to each other with the fresh medium holder, be provided in the chamber fresh medium through and be communicated with the inlet of fresh medium with the biological semi-permeable membranes boundling of hydrophilic hollow fibre that the circulation nutrient solution carries out the exchange of liquid/liquid, outlet, make fresh medium form relatively independent circulation from process in the chamber of the biological semi-permeable membranes of hydrophilic hollow fibre, when fresh medium is in through the chamber of the biological semi-permeable membranes of hydrophilic hollow fibre simultaneously, can utilize the wetting ability performance of the biological semi-permeable membranes of hydrophilic hollow fibre, carry out the exchange of nutritive substance and meta-bolites with the circulation nutrient solution in the chamber by the biological semi-permeable membranes of hydrophilic hollow fibre, increase the nutritive substance in the circulation nutrient solution, take away the meta-bolites in the circulation nutrient solution simultaneously, the nutritive ingredient of circulation nutrient solution is replenished, tissue metabolism's product obtains getting rid of, and exchanges between the liquid of simulation renal function realization nutrient solution and the liquid.Because fresh medium and circulation nutrient solution have the relatively independent separately recycle system, and can exchange mutually, therefore the circulation of nutrient solution drives and can adopt expansion pump anthropomorphic dummy systemic heart dancing pulse mode, nutrient solution is circulated provide nutrition for the tissue of cultivating.Pneumatic outlet and the gas inlet that is used for linking to each other with source of the gas are set on housing, be provided in the chamber gas through and be communicated with inlet, the outlet of gas with the biological semi-permeable membranes boundling of hydrophobic hollow fiber that the circulation nutrient solution carries out gas/liquid exchange, make and contain O
2Mixed gas can be in the chamber of biological semi-permeable membranes the time through hydrophobic hollow fiber to the circulation nutrient solution for O
2, and take away CO in the nutrient solution simultaneously
2, the simulated lung function realizes the gas/liquid exchange, promotes the renewal of nutrient solution.Because relatively independent gas passage of the biological semi-permeable membranes boundling formation of hydrophobic hollow fiber that is used for the gas process and carries out the gas/liquid exchange with the circulation nutrient solution, and liquid can not flow away from this passage, so make the chamber of housing form an enclosed space, even under the pressure of expansion pump anthropomorphic dummy systemic heart dancing pulse mode, the nutrient solution in the housing cavity can not eject housing yet.The nutrient solution gas/liquid of tissue engineering tissue bionic cultivation of the present invention and liquid/liquid interchanger forms gas/liquid and liquid/liquid integrated exchange, can realize not opening a position and just can upgrade nutrient solution, and make oxygen and carbon dioxide, fresh medium, the circulation nutrient solution finishes gas/liquid in interchanger and liquid/liquid exchange is upgraded, and nutrient solution upgrades at enclosed space internal recycle and exchange, the liquid level of nutrient solution can not produce foam yet, it is contaminated to stop nutrient solution fully, can realize nutrient solution pulsed circulation renewal again, thereby realize the blood of human body round-robin pulsed bionic cultivation of real meaning.Because nutrient solution upgrades at enclosed space internal recycle and exchange, in the tissue cultivating process, can not discharge nutrient solution, the nutrient solution total amount can not reduce, therefore just can finish the tissue engineering tissue cultivating process of long period with more a spot of fresh medium, both reduced the consumption of fresh medium, reduce cost, tissue engineering tissue is cultivated possessed the condition that industrialization is produced.And because the biological semi-permeable membranes boundling of tubular fibre is all adopted in gas/liquid and liquid/liquid exchange, the boundling that the biological semi-permeable membranes of some tubular fibres is formed can make exchange area increase, and improves exchange efficiency.As the visual plant in the tissue engineering tissue preparation system, can make the preparation of tissue engineering tissue form industrialization production with this gas/liquid and liquid/liquid interchanger.
Simultaneously, housing of the present invention adopts polycarbonate material to be made into cylindrical body or pocket, can reduce cost, and is used for clinical disposable use, avoids reusing the pollution that does not sternly cause because of cleaning and sterilizing.
The invention will be further described below in conjunction with drawings and Examples.
Description of drawings
Fig. 1 is that interchanger housing of the present invention is a kind of example structure synoptic diagram of cylindrical body;
Fig. 2 is that interchanger housing of the present invention is the another kind of example structure synoptic diagram of cylindrical body;
Fig. 3 is that interchanger housing of the present invention is a kind of example structure synoptic diagram of pocket;
Fig. 4 is that interchanger housing of the present invention is the another kind of example structure synoptic diagram of pocket
In the accompanying drawing, 1 is housing, and 2 is inner cap, and 3 is enclosing cover, 4 is the gas inlet, 5 are the fresh medium inlet, and 6 are circulation nutrient solution inlet, and 7 is the biological semi-permeable membranes boundling of hydrophilic hollow fibre, 8 is the biological semi-permeable membranes boundling of hydrophobic hollow fiber, 9 are the outlet of circulation nutrient solution, and 10 is pneumatic outlet, and 11 are the fresh medium outlet.
Embodiment
Referring to Fig. 1, the nutrient solution gas/liquid of the tissue engineering tissue bionic cultivation of present embodiment and liquid/liquid interchanger, the housing 1 that comprises the belt chamber that adopts the polycarbonate material making, the chamber of housing 1 is provided with and is used for the circulation nutrient solution inlet 6 that links to each other with culturing room and exports 9, be provided for the fresh medium inlet 5 that links to each other with the fresh medium holder on the housing 1 and export 11, be provided in the chamber fresh medium through and be communicated with the inlet 5 of fresh medium with the biological semi-permeable membranes boundling 7 of hydrophilic hollow fibre that the circulation nutrient solution carries out the exchange of liquid/liquid, outlet 11, pneumatic outlet 10 and the gas inlet 4 that is used for linking to each other with source of the gas are set on the housing 1, be provided in the chamber gas through and be communicated with gas inlet 4 with the biological semi-permeable membranes boundling 8 of hydrophobic hollow fiber that the circulation nutrient solution carries out gas/liquid exchange, outlet 10 makes the chamber of housing form enclosed space.The housing 1 of present embodiment is made as cylindrical body, be respectively arranged with on the barrel of cylindrical body respectively and export 9 connection as circulation nutrient solution inlet 6 and circulation nutrient solution, the connection and the cylindrical body of the connection of circulation nutrient solution inlet 6, circulation nutrient solution outlet 9 are one-body molded, the connection of circulation nutrient solution inlet 6 is used to connect silicone tube, and link to each other with the culturing room outlet by the expansion pump that is provided with, the connection of circulation nutrient solution outlet 9 is used for being connected with the continuous silicone tube of the import of culturing room.The two ends of described cylindrical body are provided with sealing cover respectively, and the inlet of gas and fresh medium, outlet are located at respectively on the sealing cover at cylindrical body two ends.Described sealing cover comprises inner cap 2, enclosing cover 3, and inner cap 2 is bonded in and forms sealing on the cylindrical body, and perhaps inner cap 2 is threaded on the cylindrical body and with sealing-ring and seals.Be respectively equipped with on the inner cap 2 be fixed for gas through and carry out the hole of end of the biological semi-permeable membranes boundling of hydrophobic hollow fiber of gas/liquid exchange with the circulation nutrient solution, with be fixed for fresh medium through and carry out the hole of end of the biological semi-permeable membranes boundling of hydrophilic hollow fibre of liquid/liquid exchange with the circulation nutrient solution, one of them hole is provided with connection, and to pass enclosing cover overhanging, and another hole is a through hole.The described hole that is provided with connection be fixed for gas through and carry out the hole of end of the biological semi-permeable membranes boundling of hydrophobic hollow fiber of gas/liquid exchange with the circulation nutrient solution, it is overhanging as gas inlet 4 or pneumatic outlet 10 that connection passes enclosing cover, adopt seal gum or sealing-ring sealing between connection and the enclosing cover, enclosing cover 3 be bonded in cylindrical body or in cover and form sealing, enclosing cover 3 by be threaded in cylindrical body or in cover and form sealing with sealing-ring, make between enclosing cover 3 and the inner cap 2 and form closed cavity, one-body molded another connection communicates with cavity on the enclosing cover 3, and this connection is as fresh medium inlet 5 or fresh medium outlet 11; Another hole on the inner cap 2 be fixed for gas through and carry out the through hole of end of the biological semi-permeable membranes boundling of hydrophobic hollow fiber of gas/liquid exchange with the circulation nutrient solution.Be used for gas through and be adhesively fixed on the hole that is provided with connection on the cylindrical body two ends inner cap 2 respectively with two ends that the circulation nutrient solution carries out the biological semi-permeable membranes boundling 8 of hydrophobic hollow fiber of gas/liquid exchange, described be used for fresh medium through and with two ends that the circulation nutrient solution carries out the biological semi-permeable membranes boundling 7 of hydrophilic hollow fibre of liquid/liquid exchange be adhesively fixed on respectively on the cylindrical body two ends inner cap 2 be fixed for fresh medium through and carry out the through hole of end of the biological semi-permeable membranes boundling of hydrophilic hollow fibre of liquid/liquid exchange with the circulation nutrient solution.Referring to Fig. 2, in order to improve the exchange effect, described be used for gas through and the biological semi-permeable membranes boundling 8 of hydrophobic hollow fiber that carries out the gas/liquid exchange with the circulation nutrient solution be used for fresh medium through and be staggered distributions in the chamber of housing 1 with the biological semi-permeable membranes boundling 7 of hydrophilic hollow fibre that the circulation nutrient solution carries out liquid/liquid exchange.It is good that the biological semi-permeable membranes of described hydrophilic hollow fibre, the biological semi-permeable membranes of hydrophobic hollow fiber all adopt the poly (ether sulfone) film effect.
Referring to Fig. 3, the nutrient solution gas/liquid of the tissue engineering tissue bionic cultivation of present embodiment and liquid/liquid interchanger, the housing 1 that comprises the belt chamber that adopts the polycarbonate material making, the chamber of housing 1 is provided with and is used for the circulation nutrient solution inlet 6 that links to each other with culturing room and exports 9, be provided for the fresh medium inlet 5 that links to each other with the fresh medium holder on the housing 1 and export 11, be provided in the chamber fresh medium through and be communicated with the inlet 5 of fresh medium with the biological semi-permeable membranes boundling 7 of hydrophilic hollow fibre that the circulation nutrient solution carries out the exchange of liquid/liquid, outlet 11, pneumatic outlet 10 and the gas inlet 4 that is used for linking to each other with source of the gas are set on the housing 1, be provided in the chamber gas through and be communicated with the gas inlet with the biological semi-permeable membranes boundling 8 of hydrophobic hollow fiber that the circulation nutrient solution carries out gas/liquid exchange, outlet makes the chamber of housing form enclosed space.The housing 1 of present embodiment is made as pocket, the both sides that pocket is relative are provided as the connection of circulation nutrient solution inlet 6 and circulation nutrient solution outlet 9 respectively, the connection of circulation nutrient solution inlet 6 is used to connect silicone tube, and link to each other with the culturing room outlet by the expansion pump that is provided with, the connection of circulation nutrient solution outlet 9 is used for being connected with the continuous silicone tube of the import of culturing room.The both sides that pocket is relative are provided with gas inlet 4, pneumatic outlet 10 and fresh medium inlet 5, fresh medium outlet 11 respectively, be used for gas through and be adhered to respectively in gas inlet, the outlet with two ends that the circulation nutrient solution carries out the biological semi-permeable membranes boundling 8 of hydrophobic hollow fiber of gas/liquid exchange, be used for fresh medium through and be adhered to respectively in fresh medium inlet, the outlet with two ends that the circulation nutrient solution carries out the biological semi-permeable membranes boundling 7 of hydrophilic hollow fibre of liquid/liquid exchange, the inlet of gas and fresh medium, outlet are equipped with connection.The connection of described circulation nutrient solution inlet 6, circulation nutrient solution outlet 9, gas inlet 4, pneumatic outlet 10, fresh medium inlet 5, fresh medium outlet 11 is all one-body molded with the housing of pocket, perhaps is fixedly connected on the pocket housing by heat seal.In the pocket housing, set be used for gas through and carry out the biological semi-permeable membranes boundling 8 of hydrophobic hollow fiber of gas/liquid exchange with the circulation nutrient solution and be used for fresh medium through and carry out the biological semi-permeable membranes boundling 7 of hydrophilic hollow fibre of liquid/liquid exchange with the circulation nutrient solution after, the heat seal of pocket housing is sealed.Referring to Fig. 4, in order to improve the exchange effect, described be used for gas through and the biological semi-permeable membranes boundling 8 of hydrophobic hollow fiber that carries out the gas/liquid exchange with the circulation nutrient solution be used for fresh medium through and be staggered distributions in the chamber of housing 1 with the biological semi-permeable membranes boundling 7 of hydrophilic hollow fibre that the circulation nutrient solution carries out liquid/liquid exchange.It is good that the biological semi-permeable membranes of described hydrophilic hollow fibre, the biological semi-permeable membranes of hydrophobic hollow fiber all adopt the poly (ether sulfone) film effect.
The nutrient solution gas/liquid of tissue engineering tissue bionic cultivation of the present invention and liquid/when the liquid interchanger is used in the tissue engineering tissue bionic cultivation system and device, circulation nutrient solution inlet, the outlet of this interchanger are communicated with the culturing room of tissue engineering tissue bionic cultivation system and device, by the expansion pump between circulation nutrient solution inlet and the culturing room's outlet nutrient solution are formed and circulate; The fresh medium inlet of this interchanger, outlet is communicated with the fresh medium storage bottle of tissue engineering tissue bionic cultivation system and device, by the expansion pump between fresh medium inlet and the outlet of fresh medium storage bottle fresh medium being formed circulates, fresh medium is when flowing through hydrophilic hollow fibre biology semi-permeable membranes boundling, the part fresh medium is from the chamber of the biological semipermeable membrane of hydrophilic hollow fibre outside film, take in nutritive substance to the circulation nutrient solution, the nutrient solution that circulates simultaneously also penetrates in the biological semi-permeable membranes of hydrophilic hollow fibre, meta-bolites in the circulation nutrient solution is pulled away with the fresh medium at the biological semi-permeable membranes boundling of hydrophilic hollow fibre internal recycle, forms the liquid/liquid exchange of fresh medium and circulation nutrient solution thus; The gas inlet of this interchanger by threeway respectively with O
2, CO
2, N
2Bomb communicate, pneumatic outlet is communicated with extraneous by escape pipe, O
2, CO
2, N
2Bomb under computer control, be the required mixed gas of this interchanger supply by throttling valve respectively, mixed gas enter from the gas inlet be used for gas through and carry out utilizing the dividing potential drop character of mixed gas in fresh medium and circulation nutrient solution, to take in O in the chamber of the biological semi-permeable membranes boundling of hydrophobic hollow fiber of gas/liquid exchange with the circulation nutrient solution
2, provide nutrient solution required O
2, and make CO in the circulation nutrient solution simultaneously
2Enter the biological semi-permeable membranes boundling of hydrophobic hollow fiber and discharge through escape pipe, finish the gas/liquid exchange, the pH value of nutrient solution, PCO with air-flow
2, PO
2More stable Deng culture condition, be beneficial to and cultivate histiocytic cultivation.After using the nutrient solution gas/liquid and liquid/liquid interchanger of this tissue engineering tissue bionic cultivation, drive the expansion pump that fresh medium and circulation nutrient solution circulate, the pulse mode of anthropomorphic dummy's body blood flow driving nutrient solution circulates under the control of computer, and gas/liquid and liquid/liquid integrated exchange, must not open a position and increase fresh medium and discharge cycle nutrient solution, only just can use 21 days with the 250ml fresh medium, finish once the long-term cycle of cultivating, realized the tissue engineering tissue bionic cultivation of the real meaning of medical field.
Claims (7)
1. the nutrient solution gas/liquid of a tissue engineering tissue bionic cultivation and liquid/liquid interchanger, it is characterized in that: the housing that comprises belt chamber, the chamber of housing is provided with and is used for the circulation nutrient solution entrance and exit that links to each other with culturing room, be provided for the fresh medium entrance and exit that links to each other with the fresh medium holder on the housing, be provided in the chamber fresh medium through and be communicated with the inlet of fresh medium with the biological semi-permeable membranes boundling of hydrophilic hollow fibre that the circulation nutrient solution carries out the exchange of liquid/liquid, outlet, pneumatic outlet and the gas inlet that is used for linking to each other with source of the gas are set on the housing, be provided in the chamber gas through and be communicated with the inlet of gas with the biological semi-permeable membranes boundling of hydrophobic hollow fiber that the circulation nutrient solution carries out gas/liquid exchange, outlet makes the chamber of housing form enclosed space.
2. the nutrient solution gas/liquid of tissue engineering tissue bionic cultivation according to claim 1 and liquid/liquid interchanger is characterized in that: described be used for gas through and the biological semi-permeable membranes boundling of hydrophobic hollow fiber that carries out the gas/liquid exchange with the circulation nutrient solution be used for fresh medium through and be staggered distributions in the chamber of housing with the biological semi-permeable membranes boundling of hydrophilic hollow fibre that the circulation nutrient solution carries out liquid/liquid exchange.
3. the nutrient solution gas/liquid of tissue engineering tissue bionic cultivation according to claim 1 and 2 and liquid/liquid interchanger is characterized in that: the biological semi-permeable membranes of described hydrophilic hollow fibre, the biological semi-permeable membranes of hydrophobic hollow fiber all adopt poly (ether sulfone) film.
4. the nutrient solution gas/liquid of tissue engineering tissue bionic cultivation according to claim 1 and liquid/liquid interchanger, it is characterized in that: described housing is a cylindrical body, be respectively arranged with connection on the barrel of cylindrical body as circulation nutrient solution inlet, outlet, the two ends of cylindrical body are provided with sealing cover respectively, and the inlet of gas and fresh medium, outlet are located at respectively on the sealing cover at cylindrical body two ends.
5. the nutrient solution gas/liquid of tissue engineering tissue bionic cultivation according to claim 4 and liquid/liquid interchanger, it is characterized in that: described sealing cover comprises inner cap, enclosing cover, inner cap is connected on the cylindrical body, in cover be respectively equipped be fixed for gas through and carry out the hole of end of the biological semi-permeable membranes boundling of hydrophobic hollow fiber of gas/liquid exchange with the circulation nutrient solution, with be fixed for fresh medium through and carry out the hole of end of the biological semi-permeable membranes boundling of hydrophilic hollow fibre of liquid/liquid exchange with the circulation nutrient solution, one of them hole is provided with connection, and to pass enclosing cover overhanging, another hole is a through hole, enclosing cover be connected cylindrical body or in cover, form cavity between enclosing cover and the inner cap, cover outward and be provided with another connection and communicate with cavity.
6. the nutrient solution gas/liquid of tissue engineering tissue bionic cultivation according to claim 1 and liquid/liquid interchanger, it is characterized in that: described housing is a pocket, the both sides that pocket is relative are provided as the connection of the entrance and exit of circulation nutrient solution respectively, the both sides that pocket is relative are respectively arranged with the inlet of gas and fresh medium, outlet, be used for gas through and be connected to the inlet of gas with the two ends that the circulation nutrient solution carries out the biological semi-permeable membranes boundling of hydrophobic hollow fiber of gas/liquid exchange, outlet, be used for fresh medium through and be connected to the inlet of fresh medium with the two ends that the circulation nutrient solution carries out the biological semi-permeable membranes boundling of hydrophilic hollow fibre of liquid/liquid exchange, outlet, the inlet of gas and fresh medium, outlet is equipped with connection.
7. according to the nutrient solution gas/liquid and the liquid/liquid interchanger of claim 1,2 or 4～6 arbitrary described tissue engineering tissue bionic cultivations, it is characterized in that: the material of described housing adopts polycarbonate material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101031824A CN101486967B (en) | 2009-02-13 | 2009-02-13 | Culture fluid gas/liquid and liquid/liquid exchanger for tissue engineering tissue bionic cultivation |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101031824A CN101486967B (en) | 2009-02-13 | 2009-02-13 | Culture fluid gas/liquid and liquid/liquid exchanger for tissue engineering tissue bionic cultivation |
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| Publication Number | Publication Date |
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| CN101486967A CN101486967A (en) | 2009-07-22 |
| CN101486967B true CN101486967B (en) | 2011-09-28 |
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| CN103555580B (en) * | 2013-11-11 | 2015-02-04 | 中国人民解放军第三军医大学第一附属医院 | Bionic tendon tissue incubator |
| US11306281B2 (en) | 2016-05-19 | 2022-04-19 | Koji Saito | Culture device, culture method and cultured organ produced by the culture method |
| CN106479889B (en) * | 2016-09-01 | 2019-03-22 | 奥凯（苏州）生物技术有限公司 | A kind of cell culture circulatory system |
| CN106467892B (en) * | 2016-09-01 | 2018-12-18 | 奥凯（苏州）生物技术有限公司 | A kind of automatic cytological amplification system |
| CN107805611B (en) * | 2017-11-14 | 2021-01-08 | 郑州轻工业学院 | Cell whole smoke exposure device and method |
| CN107904170A (en) * | 2017-12-18 | 2018-04-13 | 上海白泽医疗器械有限公司 | Media to cell culture modules and cell culture system |
| CN110074094B (en) * | 2019-05-24 | 2021-10-08 | 李毓光 | Organ transplantation cooling spraying treatment table |
| CN111154647A (en) * | 2020-01-17 | 2020-05-15 | 英诺维尔智能科技（苏州）有限公司 | Closed circulation external exchange biological product reactor |
| CN112940934B (en) * | 2021-01-29 | 2023-11-07 | 上海睿钰生物科技有限公司 | In-vitro life support perfusion culture system and control method thereof |
| CN112831416A (en) * | 2021-01-29 | 2021-05-25 | 上海睿钰生物科技有限公司 | In-vitro life-sustaining perfusion culture system and control method thereof |
| CN113481100A (en) * | 2021-07-14 | 2021-10-08 | 天津工业大学 | Air-permeable biological culture bottle cap and preparation method and use method thereof |
| CN114113490B (en) * | 2021-12-08 | 2023-11-10 | 中国人民解放军海军特色医学中心 | Pulmonary gas exchange simulation detection system and method in diving decompression sickness process |
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