CN111257080B - Assay specimen processing device for hepatobiliary surgery - Google Patents
Assay specimen processing device for hepatobiliary surgery Download PDFInfo
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- CN111257080B CN111257080B CN202010132067.6A CN202010132067A CN111257080B CN 111257080 B CN111257080 B CN 111257080B CN 202010132067 A CN202010132067 A CN 202010132067A CN 111257080 B CN111257080 B CN 111257080B
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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Abstract
The invention discloses an assay specimen processing device for hepatobiliary surgery, which is characterized by comprising: the device comprises a base, a control box, a conveyor belt rotating shaft, a power line, a PLC (programmable logic controller), a conveyor belt control knob, a control button, a glass slide, an inoculation telescopic rod, an inoculation transverse rod, a cell sap dropper, a scraping telescopic rod, a scraping transverse telescopic rod, a scraping cotton head connecting rod, a scraping cotton head, a cover glass telescopic rod, a cover glass transverse rod, a vacuum sucker telescopic rod and a vacuum sucker; the device design machinery is automatic, and cell inoculation, cell scrape coating, cover glass slide and make and cultivate the sample and accomplish in proper order, and the manual work only needs to carry out control operation, and whole process realizes streamlined operation, especially is fit for the preparation of large batch cell culture sample.
Description
Technical Field
The invention belongs to the field of medical specimen assay, and relates to an assay specimen processing device for hepatobiliary surgery.
Background
Hepatobiliary surgery is mainly used for studying hepatocellular carcinoma, hepatobiliary calculus, posthepatitic cirrhosis and acute liver failure caused by severe hepatitis, and is a serious disease threatening human health. Puncture cells are obtained by puncturing the lesion part, and canceration can be determined or eliminated conveniently after the cells are cultured. Cell culture requires scraping of the punctured cells on a glass slide to make a culture specimen, and observation and detection are performed after a period of culture. Present assay sample processing apparatus single structure, degree of mechanization is low, needs the manual work to carry out the knife coating to the cell, and when cultivateing the great time of cell quantity, manpower work load is big, and easily causes the knife coating inhomogeneous, influences the cell observation and the detection after cultivateing.
Disclosure of Invention
In order to solve the technical problems, the invention designs the test specimen processing device for hepatobiliary surgery, which is designed to be mechanically automated, and sequentially complete cell inoculation, cell scraping and cover glass slide preparation and culture specimen preparation, only needs to be manually controlled, realizes streamlined operation in the whole process, and is particularly suitable for large-batch cell culture specimen preparation.
In order to realize the technical effects, the invention is realized by the following technical scheme: an assay sample processing device for hepatobiliary surgery, comprising: the device comprises a base, a control box, a conveyor belt rotating shaft, a power line, a PLC (programmable logic controller), a conveyor belt control knob, a control button, a glass slide, an inoculation telescopic rod, an inoculation transverse rod, a cell sap dropper, a scraping telescopic rod, a scraping transverse telescopic rod, a scraping cotton head connecting rod, a scraping cotton head, a cover glass telescopic rod, a cover glass transverse rod, a vacuum sucker telescopic rod and a vacuum sucker;
the base structure is a cuboid, the upper end face of the base structure is open, and the base structure is hollow; the right end face of the base is connected with a control box; the left side and the right side in the base are respectively provided with a conveying belt rotating shaft, and the conveying belt rotating shafts are connected with conveying belts; a power supply is arranged in the control box and connected to each electromechanical component, and a power line is led out from the inside and connected to the right side surface of the control box; a PLC controller is arranged in the control box; the upper surface of the control box is connected with a conveyor belt control knob; the front of the control box is provided with control buttons of all parts; placing a glass slide on the conveying belt; the left side behind the base is fixedly connected with an inoculation telescopic rod, the head end of the inoculation telescopic rod is fixedly connected with an inoculation cross rod, the head end of the inoculation cross rod is provided with a through hole, and a cell sap dropper is detachably connected in the through hole; the middle part of the back of the base is fixedly connected with a scraping telescopic rod, the head end of the scraping telescopic rod is fixedly connected with a scraping transverse rod, the interior of the scraping transverse rod is telescopically connected with a scraping transverse telescopic rod, the head end of the scraping transverse telescopic rod is fixedly connected with a scraping cotton head connecting rod, and the lower end of the scraping cotton head connecting rod is detachably connected with a scraping cotton head; the right side of the back of the base is fixedly connected with a cover glass telescopic rod, the head end of the cover glass telescopic rod is fixedly connected with a cover glass cross rod, the head end of the cover glass cross rod is fixedly connected with two vacuum sucker telescopic rods, and the lower ends of the vacuum sucker telescopic rods are connected with vacuum suckers;
furthermore, the PLC is connected with a conveyor belt rotating shaft, the conveyor belt rotating shaft is connected with a conveyor belt control knob through a power cord, the PLC is preset with a program, the conveyor belt rotating shaft is controlled to rotate to a specific position and then automatically stops, and the conveyor belt control knob needs to be manually controlled to rotate;
furthermore, three rows of control buttons are vertically arranged and respectively correspond to three functions of controlling inoculation, blade coating and cover glass;
furthermore, the vacuum chuck is used for sucking the glass slide when the vacuum chuck is powered on and dropping the glass slide when the vacuum chuck is powered off;
further, the scraping and coating cotton head is in a strip shape;
the invention also aims to provide a quantifiable cell sap dropper which consists of a push rod grab handle, a refrigeration outer cylinder, a push rod, a piston, a spring limiting part, a spring pressing block, a liquid adding opening, a quantitative liquid storage part switch and a dropper head; the refrigerating outer cylinder has a refrigerating function, the lower end of the refrigerating outer cylinder is connected with a quantitative liquid storage part, the quantitative liquid storage part is controlled by a switch, and the lower end of the quantitative liquid storage part is provided with a liquid dropper; the refrigeration outer barrel is fixedly connected with a spring limiting part close to the upper end part, a through hole is formed in the middle of the spring limiting part, and a spring is fixedly connected to the spring limiting part; a liquid feeding port is connected to the refrigerating outer cylinder; the push rod penetrates through the upper end of the refrigeration outer barrel and the spring limiting part and reaches the lower end of the refrigeration outer barrel, the lower end of the push rod is connected with the piston, and the outer diameter of the piston is equal to the inner diameter of the refrigeration outer barrel; the upper part of the push rod is fixedly connected with a spring pressing block;
the quantitative cell sap dropper has the working principle that: adding cell liquid into the refrigeration outer cylinder from the liquid adding port, pressing the push rod grab handle, and driving the push rod, the spring pressing block and the piston to move downwards together; the spring is extruded and deformed, the piston is pushed into the quantitative liquid storage part, the cell liquid starts to flow into the quantitative liquid storage part, after the quantitative liquid storage part is filled with the cell liquid, the handle of the push rod is loosened, the spring is restored, the spring drives the spring to press the pressing block, the push rod is driven, the piston is upward, and the piston plugs the lower end of the refrigeration outer cylinder; the quantitative liquid storage part stores quantitative cell liquid; the quantitative liquid storage part is controlled to be opened and closed, so that the cell sap flows out of the dropping head.
Another object of the present invention is to provide a method of using an assay sample processing device for hepatobiliary surgery, the method comprising:
1. connecting a device power cord to a power source;
2. cleaning the conveying belt to clean the surface, and placing the glass slide on the conveying belt and right below the cell sap dropper, or placing a clean isolation pad under the glass slide;
3. controlling a button of an inoculation telescopic rod to drive a cell sap dropper to descend to a proper height above the glass slide;
4. pressing the handle part of the push rod of the cell sap dropper to enable cell sap in the refrigeration outer cylinder to enter the quantitative liquid storage part, loosening the handle part of the push rod, storing the quantitative cell sap in the quantitative liquid storage part, and controlling the switch of the quantitative liquid storage part to enable the cell sap to be dripped in the middle of the glass slide;
5. operating a conveyor belt control knob, moving the glass slide to the position right below the scraping and coating cotton head on the conveyor belt, and automatically stopping the conveyor belt;
6. controlling a scraping telescopic rod control button to drive a scraping cotton head to descend to a proper height above the glass slide, and controlling a scraping transverse telescopic rod button to stretch back and forth to uniformly scrape and coat the cell sap;
7. operating the control knob of the conveyor belt to ensure that the conveyor belt automatically stops when the conveyor belt continuously drives the glass slide to rotate to the position right below the cover glass;
8. controlling a control button of a telescopic rod of the cover glass to enable the cover glass to be driven to descend to a proper height above the glass slide, controlling a telescopic rod button of a vacuum chuck to descend to enable one telescopic rod of the vacuum chuck to enable the cover glass to be attached to one side of the glass slide, and then enabling one telescopic rod of the vacuum chuck to descend to enable the cover glass to be slowly covered above the glass slide;
9. the electricity of the vacuum chuck is cut off, so that the chuck loosens the cover glass;
the invention has the beneficial effects that: the laboratory sample processing device for hepatobiliary surgery is mechanized in structure, and can perform inoculation, scraping and cover glass streamlined operation, wherein each part of the laboratory sample processing device only needs to be manually operated by a button without manual inoculation, scraping and cover glass; the quantitative cell sap dropper can ensure that the cell sap dripped on the glass slide is uniform every time, so that the phenomenon of nonuniform volume caused by manual inoculation is avoided, and the cell sap dropper adopts a refrigeration outer barrel, so that the cell sap in the cell sap dropper can be in a low-level environment, and the cell activity is kept; the automatic scraping function is matched with the strip-shaped scraping cotton head, so that the cell can be uniformly scraped, and the uniformity of the cell cannot be ensured by manual scraping; the cover glass needs to be carefully covered, when a large number of cell culture samples are manufactured, the manual work load is increased by manual cover glass, the manual cover glass is saved by automatic cover glass, and the cover glass can be accurately placed above the glass slide; the conveying belt controlled by the PLC can enable the glass slide driven by the conveying belt to stop at the same position every time, and therefore blade coating and accurate operation of cover glass are facilitated.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic view showing the construction of an assay sample processing device for hepatobiliary surgery;
FIG. 2 is a schematic view showing the internal structure of an assay sample processing device for hepatobiliary surgery;
FIG. 3 is a schematic view of a cell drip tube structure of an assay sample processing device for hepatobiliary surgery 1;
FIG. 4 is a schematic diagram of a pipette structure of the specimen processing device for hepatobiliary surgery, FIG. 2;
FIG. 5 is a schematic structural view of example 4 of an assay specimen processing device for hepatobiliary surgery;
in the drawings, the reference numbers indicate the following list of parts:
1-base, 2-control box, 3-conveyor belt, 4-conveyor belt rotating shaft, 5-power line, 6-PLC controller, 7-conveyor belt control knob, 8-control button, 9-glass slide, 10-inoculation telescopic rod, 11-inoculation transverse rod, 12-cell sap dropper, 121-push rod handle, 122-refrigeration outer cylinder, 123-push rod, 124-piston, 125-spring limiting part, 126-spring, 127-spring pressing block, 128-liquid adding port, 129-quantitative liquid storage part, 1210-quantitative liquid storage part switch, 1211-liquid dropping head, 13-knife coating telescopic rod, 14-knife coating transverse rod, 15-knife coating transverse telescopic rod, 16-knife coating cotton head connecting rod, 17-knife coating cotton head, 18-glass cover telescopic rod, 19-glass cover transverse rod, 20-vacuum suction cup telescopic rod, 21-vacuum suction cup, and 22-limit switch.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
Referring to fig. 1 to 4, an assay sample processing device for hepatobiliary surgery is characterized by comprising: the cell culture device comprises a base 1, a control box 2, a conveyor belt 3, a conveyor belt rotating shaft 4, a power line 5, a PLC (programmable logic controller) 6, a conveyor belt control knob 7, a control button 8, a glass slide 9, an inoculation telescopic rod 10, an inoculation transverse rod 11, a cell sap dropper 12, a scraping telescopic rod 13, a scraping transverse rod 14, a scraping transverse telescopic rod 15, a scraping cotton head connecting rod 16, a scraping cotton head 17, a cover glass telescopic rod 18, a cover glass transverse rod 19, a vacuum sucker telescopic rod 20 and a vacuum sucker 21;
the base 1 is in a cuboid structure, the upper end face of the base is open, and the interior of the base is hollow; the right end face of the base 1 is connected with a control box 2; a conveyor belt rotating shaft 4 is respectively arranged at the left side and the right side in the base 1, and the conveyor belt 3 is connected to the conveyor belt rotating shaft 4; a power supply is arranged in the control box 2 and connected to each electromechanical component, and a power line 5 is led out from the inside and connected to the right side surface of the control box 2; a PLC (programmable logic controller) 6 is installed in the control box 2; the upper surface of the control box 2 is connected with a conveyor belt control knob 7; the front of the control box 2 is provided with a control button 8 of each component; the slide glass 9 is placed on the conveyor belt 3; the left side of the back of the base 1 is fixedly connected with an inoculation telescopic rod 10, the head end of the inoculation telescopic rod 10 is fixedly connected with an inoculation cross rod 11, the head end of the inoculation cross rod 11 is provided with a through hole, and a cell sap dropper 12 is detachably connected in the through hole; a scraping telescopic rod 13 is fixedly connected to the middle of the back of the base 1, a scraping transverse rod 14 is fixedly connected to the head end of the scraping telescopic rod 13, a scraping transverse telescopic rod 15 is telescopically connected to the inside of the scraping transverse rod 14, a scraping cotton head connecting rod 16 is fixedly connected to the head end of the scraping transverse telescopic rod 15, and a scraping cotton head 17 is detachably connected to the lower end of the scraping cotton head connecting rod 16; the right side of the back of the base 1 is fixedly connected with a cover glass telescopic rod 18, the head end of the cover glass telescopic rod 18 is fixedly connected with a cover glass cross rod 19, the head end of the cover glass cross rod 19 is fixedly connected with two vacuum chuck telescopic rods 20, and the lower ends of the vacuum chuck telescopic rods 20 are connected with a vacuum chuck 21;
the PLC controller 6 is connected with the conveyor belt rotating shaft 4, the conveyor belt rotating shaft 4 is connected with the conveyor belt control knob 7 through the power cord 5, the PLC controller 6 is provided with a program in advance, the conveyor belt rotating shaft 4 is controlled to rotate to a specific position and stop automatically, and the conveyor belt control knob 7 needs to be controlled manually to rotate; the position where the slide glass 9 stops on the conveyor belt 3 is just opposite to the position where the cotton head 17 and the cover glass are scraped;
three rows of control buttons 8 are vertically arranged and respectively correspond to three parts of functions of inoculation, blade coating and cover glass control;
the vacuum sucker 21 sucks the glass slide when the power is on, and the glass slide falls when the power is off;
the scraping cotton head 17 is in a strip shape; the scrape coating cotton head 17 can be replaced, and the scrape coating is even guaranteed by the long-strip-shaped cotton head.
Example 2
A kind of hepatobiliary surgery uses the laboratory sample processing unit, its quantitative cell sap dropper 12, by the push rod grab handle 121, refrigerate outer cylinder 122, push rod 123, piston 124, spring spacing department 125, spring 126, spring pressing block 127, liquid filling opening 128, quantitative liquid storage part 129, quantitative liquid storage part switch 1210, drip head 1211 to make up; the refrigerating outer cylinder 122 has refrigerating function, the lower end is connected with a quantitative liquid storage part 129 and controlled by a quantitative liquid storage part switch 1210, and the lower end of the quantitative liquid storage part 129 is provided with a liquid dripping head 1211; the refrigeration outer cylinder 122 is fixedly connected with a spring limiting part 125 near the upper end part, a through hole is formed in the middle of the spring limiting part 125, and a spring 126 is fixedly connected on the spring limiting part 125; the refrigerating outer cylinder 122 is connected with a liquid adding port 128; the push rod 123 penetrates through the upper end of the refrigeration outer cylinder 122 and the spring limiting part 125 to reach the lower end of the refrigeration outer cylinder 122, the lower end of the push rod 123 is connected with the piston 124, and the outer diameter of the piston 124 is equal to the inner diameter of the refrigeration outer cylinder 122; the upper part of the push rod 123 is fixedly connected with a spring pressing block 127;
the cell sap dropper 12 capable of being quantified has the working principle that: cell liquid is added into the refrigerating outer cylinder 122 from the liquid adding port 128, and the push rod handle 121 is pressed to drive the push rod 123, the spring pressing block 127 and the piston 124 to move downwards together; the spring 126 is extruded and deformed, the piston 124 is pushed into the quantitative liquid storage part 129, the cell liquid starts to flow into the quantitative liquid storage part 129, when the quantitative liquid storage part 129 is filled with the cell liquid, the push rod handle 121 is released, the spring 126 is restored, the spring pressing block 127 and the push rod 123 are driven, the piston 124 is upwards, and the piston 124 blocks the lower end of the refrigerating outer cylinder 122; the quantitative liquid storage portion 129 stores a quantitative amount of cell liquid; the quantitative liquid reservoir switch 1210 is controlled to allow the cell liquid to flow out from the dripper 1211.
Example 3
A use method of an assay sample processing device for hepatobiliary surgery comprises the following steps:
1. connecting the device power cord 5 to a power source;
2. cleaning the conveying belt 3 to clean the surface, and placing the glass slide 9 on the conveying belt 3 and right below the cell sap dropper 12, or placing a clean isolation pad under the glass slide 9;
3. a button 10 of the inoculation telescopic rod is controlled to drive a cell sap dropper 12 to descend to a proper height above the glass slide 9;
4. pressing the push rod handle 121 part of the cell sap dropper 12 to make the cell sap in the refrigeration outer cylinder 122 enter the liquid storage part 129 filled with quantitative liquid, loosening the push rod handle 121 part to store the quantitative cell sap in the liquid storage part 129, controlling the switch 1210 of the liquid storage part to make the cell drip in the middle of the glass slide 9;
5. the conveyor belt control knob 7 is operated, the glass slide 9 moves under the scraping cotton coating head 17 on the conveyor belt 3, and the conveyor belt 3 automatically stops;
6. controlling a button of a scraping telescopic rod 13 to drive a scraping cotton head 17 to descend to a proper height above the glass slide 9, and controlling a button of a scraping transverse telescopic rod 15 to stretch back and forth to scrape cell sap uniformly;
7. operating the conveyor belt control knob 7 to enable the conveyor belt 3 to continuously drive the glass slide 9 to rotate to the position right below the cover glass, and then automatically stopping the conveyor belt 3;
8. controlling a control button of a cover glass telescopic rod 18 to enable the cover glass to be lowered to a proper height above a glass slide 9, controlling a button of a vacuum sucker telescopic rod 20 to lower one vacuum sucker telescopic rod 20 to enable one side of the cover glass to be attached to one side of the glass slide 9, and then lowering the other vacuum sucker telescopic rod 20 to enable the cover glass to slowly cover the upper side of the glass slide 9;
9. the vacuum chuck 21 is de-energized, causing the chuck to release the coverslip.
Example 4
Referring to fig. 1 to 4, an assay sample processing device for hepatobiliary surgery includes: the cell culture device comprises a base 1, a control box 2, a conveyor belt 3, a conveyor belt rotating shaft 4, a power line 5, a PLC (programmable logic controller) 6, a conveyor belt control knob 7, a control button 8, a glass slide 9, an inoculation telescopic rod 10, an inoculation transverse rod 11, a cell sap dropper 12, a scraping telescopic rod 13, a scraping transverse rod 14, a scraping transverse telescopic rod 15, a scraping cotton head connecting rod 16, a scraping cotton head 17, a cover glass telescopic rod 18, a cover glass transverse rod 19, a vacuum sucker telescopic rod 20, a vacuum sucker 21 and a limit switch 22;
the base 1 is in a cuboid structure, the upper end face of the base is open, and the base is hollow; the right end face of the base 1 is connected with a control box 2; a conveyor belt rotating shaft 4 is respectively arranged at the left side and the right side in the base 1, and the conveyor belt 3 is connected to the conveyor belt rotating shaft 4; a power supply is arranged in the control box 2 and connected to each electromechanical component, and a power line 5 is led out from the inside and connected to the right side surface of the control box 2; a PLC (programmable logic controller) 6 is arranged in the control box 2; the upper surface of the control box 2 is connected with a conveyor belt control knob 7; the front of the control box 2 is provided with a control button 8 of each component; a slide glass 9 is placed on the conveyor belt 3; the left side of the back of the base 1 is fixedly connected with an inoculation telescopic rod 10, the head end of the inoculation telescopic rod 10 is fixedly connected with an inoculation cross rod 11, the head end of the inoculation cross rod 11 is provided with a through hole, and a cell sap dropper 12 is detachably connected in the through hole; a scraping and coating telescopic rod 13 is fixedly connected to the middle of the back of the base 1, a scraping and coating transverse rod 14 is fixedly connected to the head end of the scraping and coating telescopic rod 13, a scraping and coating transverse telescopic rod 15 is telescopically connected to the inside of the scraping and coating transverse rod 14, a scraping and coating cotton head connecting rod 16 is fixedly connected to the head end of the scraping and coating transverse telescopic rod 15, and a scraping and coating cotton head 17 is detachably connected to the lower end of the scraping and coating cotton head connecting rod 16; the right side of the back of the base 1 is fixedly connected with a cover glass telescopic rod 18, the head end of the cover glass telescopic rod 18 is fixedly connected with a cover glass cross rod 19, the head end of the cover glass cross rod 19 is fixedly connected with two vacuum chuck telescopic rods 20, and the lower ends of the vacuum chuck telescopic rods 20 are connected with a vacuum chuck 21; two ends of the upper surface of the base 1 are respectively provided with a limit switch 22, and the limit switches 22 are connected with the conveyor belt rotating shaft 4;
the PLC 6 is connected with a conveyor belt rotating shaft 4, the conveyor belt rotating shaft 4 is connected with a conveyor belt control knob 7 through a power cord 5, a limit switch 22 is connected with the conveyor belt rotating shaft 4, the conveyor belt control knob 7 is a master switch, a program is preset in the PLC 6, the conveyor belt rotating shaft 4 is controlled to rotate to a specific position and automatically stop, and the conveyor belt control knob 7 needs to be manually controlled to rotate; the position of the slide glass 9 stopped on the conveyor belt 3 is just opposite to the position of the scraping cotton head 17 and the cover glass; when the conveyor belt 3 drives the glass slide 9 to move to the position below the rightmost cover glass and stop, the conveyor belt control knob 7 is started again, and the conveyor belt rotating shaft 4 rotates to the left under the action of the right limit switch 22, so that the conveyor belt 3 is driven to rotate to the left;
three rows of control buttons 8 are vertically arranged and respectively correspond to three functions of inoculation, blade coating and cover glass control;
the vacuum chuck 21 sucks the glass slide when the power is on, and the glass slide falls when the power is off;
the scraping cotton head 17 is in a strip shape; the scrape coating cotton head 17 can be replaced, and the scrape coating is even guaranteed by the long-strip-shaped cotton head.
In conclusion, the laboratory sample processing device for hepatobiliary surgery is mechanized in structure, can perform inoculation, scraping and cover glass streamlined operation, only needs to manually perform button operation on each component, and does not need manual inoculation, scraping and cover glass; the quantitative cell sap dropper can ensure that the cell sap dripped on the glass slide is uniformly measured each time, so that the phenomenon of nonuniform measurement caused by manual inoculation is avoided, and the cell sap dropper adopts a refrigeration outer barrel, so that the internal cell sap can be in a low-level environment, and the cell activity is kept; the automatic scraping function is matched with the strip-shaped scraping cotton head, so that the cell can be uniformly scraped, and the uniformity of the cell cannot be ensured by manual scraping; the cover glass needs to be carefully covered, when a large number of cell culture samples are manufactured, the manual work load is increased by manual cover glass, the manual cover glass is saved by automatic cover glass, and the cover glass can be accurately placed above the glass slide; the PLC-controlled conveying belt can enable the glass slides driven by the conveying belt to stop at the same position every time, and blade coating and accurate operation of cover glass are facilitated.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.
Claims (2)
1. An assay sample processing device for hepatobiliary surgery, comprising: the device comprises a base (1), a control box (2), a conveyor belt (3), a conveyor belt rotating shaft (4), a power line (5), a PLC (programmable logic controller) (6), a conveyor belt control knob (7), a control button (8), a glass slide (9), an inoculation telescopic rod (10), an inoculation transverse rod (11), a cell sap dropper (12), a scraping telescopic rod (13), a scraping transverse rod (14), a scraping transverse telescopic rod (15), a scraping cotton head connecting rod (16), a scraping cotton head (17), a cover glass telescopic rod (18), a cover glass transverse rod (19), a vacuum sucker telescopic rod (20) and a vacuum sucker (21);
the base (1) is in a cuboid structure, the upper end face of the base is open, and the interior of the base is hollow; the right end face of the base (1) is connected with a control box (2); a conveying belt rotating shaft (4) is respectively arranged at the left side and the right side in the base (1), and the conveying belt (3) is connected to the conveying belt rotating shaft (4); a power supply is arranged in the control box (2) and connected to each electromechanical component, and a power line (5) is led out from the inside and connected to the right side surface of the control box (2); a PLC (programmable logic controller) controller (6) is arranged in the control box (2); the upper surface of the control box (2) is connected with a conveyor belt control knob (7); the front of the control box (2) is provided with a control button (8) of each component; a slide glass (9) is placed on the conveyor belt (3); the left side of the back of the base (1) is fixedly connected with an inoculation telescopic rod (10), the head end of the inoculation telescopic rod (10) is fixedly connected with an inoculation cross rod (11), the head end of the inoculation cross rod (11) is provided with a through hole, and a cell sap dropper (12) is detachably connected in the through hole; a scraping and coating telescopic rod (13) is fixedly connected to the middle of the back of the base (1), a scraping and coating transverse rod (14) is fixedly connected to the head end of the scraping and coating telescopic rod (13), a scraping and coating transverse telescopic rod (15) is telescopically connected into the scraping and coating transverse rod (14), a scraping and coating cotton head connecting rod (16) is fixedly connected to the head end of the scraping and coating transverse telescopic rod (15), and a scraping and coating cotton head (17) is detachably connected to the lower end of the scraping and coating cotton head connecting rod (16); the right side of the back of the base (1) is fixedly connected with a cover glass telescopic rod (18), the head end of the cover glass telescopic rod (18) is fixedly connected with a cover glass cross rod (19), the head end of the cover glass cross rod (19) is fixedly connected with two vacuum sucker telescopic rods (20), and the lower ends of the vacuum sucker telescopic rods (20) are connected with a vacuum sucker (21);
the PLC controller (6) is connected with the conveyor belt rotating shaft (4), the conveyor belt rotating shaft (4) is connected with the conveyor belt control knob (7) through the power cord (5), a program is preset in the PLC controller (6), the conveyor belt rotating shaft (4) is controlled to rotate to a specific position and automatically stop, and the conveyor belt control knob (7) needs to be manually controlled to rotate;
three rows of control buttons (8) are vertically arranged and respectively correspond to three parts of functions of inoculation, blade coating and cover glass;
the vacuum sucker (21) sucks the glass slide when being powered on, and the glass slide falls when the power is off;
the scraping cotton head (17) is in a strip shape;
the cell sap dropper (12) consists of a push rod grab handle (121), a refrigeration outer cylinder (122), a push rod (123), a piston (124), a spring limiting part (125), a spring (126), a spring pressing block (127), a liquid adding port (128), a quantitative liquid storage part (129), a quantitative liquid storage part switch (1210) and a dropper (1211); the refrigerating outer cylinder (122) has refrigerating function, the lower end of the refrigerating outer cylinder is connected with a quantitative liquid storage part (129) and is controlled by a quantitative liquid storage part switch (1210), and the lower end of the quantitative liquid storage part (129) is provided with a liquid dropper (1211); the refrigeration outer cylinder (122) is fixedly connected with a spring limiting part (125) close to the upper end part, a through hole is formed in the middle of the spring limiting part (125), and a spring (126) is fixedly connected on the spring limiting part (125); a liquid adding port (128) is connected to the refrigerating outer cylinder (122); the push rod (123) penetrates through the upper end of the refrigeration outer cylinder (122) and the spring limiting part (125) and reaches the lower end of the refrigeration outer cylinder (122), the lower end of the push rod (123) is connected with the piston (124), and the outer diameter of the piston (124) is equal to the inner diameter of the refrigeration outer cylinder (122); the upper part of the push rod (123) is fixedly connected with a spring pressing block (127).
2. The device of claim 1, wherein the device is used by the method comprising:
1. connecting a device power cord (5) to a power source;
2. cleaning the conveyor belt (3) to clean the surface, and placing the glass slide (9) on the conveyor belt (3) and right below the cell sap dropper (12), or padding a clean isolation pad under the glass slide (9);
3. a button of an inoculation telescopic rod (10) is controlled to drive a cell sap dropper (12) to descend to a proper height above a glass slide (9);
4. pressing a push rod handle (121) part of a cell sap dropper (12) to enable cell sap in a refrigerating outer cylinder (122) to enter a quantitative liquid storage part (129) filled with the cell sap, loosening the push rod handle (121) part to store the quantitative cell sap in the quantitative liquid storage part (129), and controlling a quantitative liquid storage part switch (1210) to enable the cell sap to be dripped in the middle part of a glass slide (9);
5. operating a conveyor belt control knob (7), moving the glass slide (9) under the scraping cotton head (17) on the conveyor belt (3), and automatically stopping the conveyor belt (3);
6. controlling a control button of a scraping and coating telescopic rod (13) to drive a scraping and coating cotton head (17) to descend to a proper height above a glass slide (9), and controlling a scraping and coating transverse telescopic rod (15) button to stretch back and forth to uniformly scrape and coat cell sap;
7. operating a conveyor belt control knob (7) to enable the conveyor belt (3) to continuously rotate to the position right below the cover glass with the slide glass (9), and then automatically stopping the conveyor belt (3);
8. controlling a control button of a cover glass telescopic rod (18) to enable the cover glass to be lowered to a proper height on a glass slide (9) with the cover glass, controlling a button of a vacuum chuck telescopic rod (20), firstly lowering the vacuum chuck telescopic rod (20) to enable one side of the cover glass to be attached to one side of the glass slide (9), and then lowering the vacuum chuck telescopic rod (20) to enable the cover glass to be slowly covered above the glass slide (9);
9. the vacuum chuck (21) is de-energized, causing the chuck to release the coverslip.
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| CN202010132067.6A CN111257080B (en) | 2020-02-29 | 2020-02-29 | Assay specimen processing device for hepatobiliary surgery |
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| CN202010132067.6A CN111257080B (en) | 2020-02-29 | 2020-02-29 | Assay specimen processing device for hepatobiliary surgery |
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| CN111257080A CN111257080A (en) | 2020-06-09 |
| CN111257080B true CN111257080B (en) | 2023-03-28 |
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| CN113848098B (en) * | 2021-08-13 | 2024-05-17 | 青岛市胶州中心医院 | Sample treatment equipment for hepatobiliary surgery |
| CN115069669B (en) * | 2022-07-28 | 2022-11-04 | 山东第一医科大学附属省立医院(山东省立医院) | Laboratory sample waste treatment equipment suitable for hepatobiliary surgery |
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