CN108060082A - A kind of stem cell filter separator - Google Patents

A kind of stem cell filter separator Download PDF

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Publication number
CN108060082A
CN108060082A CN201711489074.6A CN201711489074A CN108060082A CN 108060082 A CN108060082 A CN 108060082A CN 201711489074 A CN201711489074 A CN 201711489074A CN 108060082 A CN108060082 A CN 108060082A
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CN
China
Prior art keywords
filter
stem cell
drive link
filter device
driving motor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201711489074.6A
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Chinese (zh)
Inventor
徐悦
鲁振宇
韩洪起
张冰晶
秦臻
王瑶
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tianjin Puri Purcell Biotechnology Co Ltd
Original Assignee
Tianjin Puri Purcell Biotechnology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Tianjin Puri Purcell Biotechnology Co Ltd filed Critical Tianjin Puri Purcell Biotechnology Co Ltd
Priority to CN201711489074.6A priority Critical patent/CN108060082A/en
Publication of CN108060082A publication Critical patent/CN108060082A/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/18Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/24Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04Cell isolation or sorting

Abstract

The object of the present invention is to provide a kind of stem cell filter separator, including:Housing crushes filter device, driving motor, electric machine main shaft, drive link, transmission shaft, speed reducer, stirring shaft, upper agitating vane, lower agitating vane, filter screen, filter membrane, support arm, the first shock-absorption backing plate, support plate, support base, catabolic enzyme ascending pipe and automatic heating device, it is characterised in that:Driving motor is arranged on the one side of housing, and drive link is arranged on the one side of driving motor, and electric machine main shaft is arranged between driving motor and drive link, and driving motor is connected with each other by electric machine main shaft and drive link, and speed reducer is arranged on drive link one side.The present invention is provided with agitating vane, lower agitating vane and screw blade on stirring shaft, will not because shear history machinery exert oneself it is uneven caused by a large amount of cells damage, stem cell liquid can be stirred at many levels, so that stirring is evenly, it prevents stem cell particle from blocking filter screen, improves the efficiency of separation and screening.

Description

A kind of stem cell filter separator
Technical field
The present invention relates to stem cell filtration and separation techniques field more particularly to a kind of stem cell filter separators.
Background technology
At present, stem cell refers to the initial cell with self-renewing and multi-lineage potential, is the cells of origin of body, It can break up under given conditions as one or more cells for forming tissue or organ.Self-renewing refers to stem cell The size of stem cell population can be maintained by cell division;Multidirectional Differentiation refers to that stem cell has plasticity, in different rings Under border, different histocytes can be divided into so as to form the histoorgan of the various complexity of body.Fat stem cell is in recent years Carry out isolated a kind of stem cell with multi-lineage potential from adipose tissue, can break up under specific inductive condition For osteoblast, chondroblast and adipocyte etc..Neural stem cell (Neural Stem Cell, NSCs) is stem cell One kind, neural stem cell play a significant role in neurodevelopment and reparation damaged nerve tissue.Neural stem cells transplantation is to repair Effective ways that are multiple and replacing injured cerebral tissue, can rebuild partial loop and function.In addition neural stem cell can be used as gene to carry Body for the gene therapy of intracranial tumors and other neurological diseases, by the use of neural stem cell as gene therapy vector, compensates for The some shortcomings of viral vectors.In addition, neural stem cell is alternatively arranged as a kind of cell model to evaluate the toxicity of new drug and pharmacology Effect.Due to the equipment of extraction stem cell, its is complicated, and equipment weight, volume is big, the limitation of price high condition, only few at present Amount is applied to clinic, while the shear history of cell is typically manually to carry out, and can cause the damage of a large amount of cells, reduces dry thin The survival rate of born of the same parents causes blood waste, and the most mescenchymal stem cell separator of application is cell screen clothes at present, is passed through It rocks, stir and shakes to reach cell purpose separated with postdigestive tissue block, this method for separating stem cell, which exists, to be divided From less efficient, stir uneven and stem cell and be vulnerable to pollution.
The content of the invention
In view of the above-mentioned problems, the object of the present invention is to provide a kind of stem cell filter separators, the present invention is in stirring shaft On be provided with agitating vane, lower agitating vane and screw blade, will not be a large amount of thin caused by shear history machinery exerts oneself unevenness The damage of born of the same parents can at many levels be stirred stem cell liquid so that stirring evenly, prevents stem cell particle from blocking sieves Net improves the efficiency of separation and screening, and the present invention, which is provided with, holds the volume flow that flowmeter is used to measure stem cell solution, this hair Bright filter screen is V-arrangement stainless steel filter screen, has not only reduced the surface tension of its upper fluid, but also increases carrying liquid Surface area, volume and liquid lower floor pressure, are more advantageous under liquid flow, significantly improve the filter efficiency of stem cell, the present invention Seal rubber plug is provided with, can be effectively sealed to crushing filter device, the present invention is provided with degerming on liquid suction pipe Filter reduces the chance that pollution occurs, and improves the survival rate of stem cell.
The used to solve above-mentioned technical problem technical solution of the present invention is:A kind of stem cell filter separator, including: Housing, crush filter device, driving motor, electric machine main shaft, drive link, transmission shaft, speed reducer, stirring shaft, upper agitating vane, Lower agitating vane, filter screen, filter membrane, support arm, the first shock-absorption backing plate, support plate, support base and catabolic enzyme ascending pipe, It is characterized in that:The driving motor is arranged on the one side of the housing, and the drive link is arranged on the one of the driving motor Side, the electric machine main shaft are arranged between the driving motor and drive link, the driving motor by the electric machine main shaft with The drive link is connected with each other, and the speed reducer is arranged on the one side of the drive link, the transmission shaft be arranged on speed reducer with Between drive link, the drive link is connected with each other by transmission shaft and speed reducer, and the stirring shaft is arranged on the speed reducer Lower end, the stirring shaft and the speed reducer be connected with each other, the inside for crushing filter device and being arranged on the housing, The seal rubber plug is arranged on the upper end of the crushing filter device, and the seal rubber plug is mutually interconnected with the crushing filter device It connects, the lower end of the stirring shaft passes through the seal rubber plug and stretches into the inside for crushing filter device, the upper agitating vane Be arranged at the lower agitating vane on the stirring shaft, upper agitating vane and lower agitating vane with the stirring shaft It is mutually permanently connected, the filter screen is arranged on the lower end of the lower agitating vane, and the filter screen crushed with described Filter device is is detachably connected, and the filter membrane is arranged on the lower end of the filter screen, and the filter membrane crushed with described It filters device to be connected with each other, the support arm is arranged on the lower end of the crushing filter device, and first shock-absorption backing plate is arranged on The lower end of the support arm, first shock-absorption backing plate are mutually permanently connected with the support arm, and the support plate is arranged on institute The lower end of the first shock-absorption backing plate is stated, support plate is mutually permanently connected with the first shock-absorption backing plate, and the support base is arranged on described The lower end of housing, the catabolic enzyme ascending pipe are arranged on the upper end of the crushing filter device, the catabolic enzyme ascending pipe and powder Broken filter device is interconnected.
Further, the upper end for crushing filter device is provided with cell liquid ascending pipe, and solenoid valve is arranged on cell liquid note Enter on pipe, the lower end of the cell liquid ascending pipe is interconnected with being arranged on the cell liquid inlet opened up on seal rubber plug, institute The one side for stating crushing filter device is provided with liquid suction pipe, and drawing liquid control valve, drawing liquid pump and flowmeter are provided on liquid suction pipe, described One end of liquid suction pipe is interconnected with the crushing filter device, and the one side of the liquid suction pipe is provided with separator box, liquid suction pipe The other end is interconnected with separator box.
Further, it is provided with sterilizing filter on the liquid suction pipe.
Further, it is provided with supporting bracket between the support arm and crushing filter device.
Further, the filter screen is V-arrangement stainless steel filter screen.
Further, screw blade is provided on the stirring shaft, screw blade is mutually permanently connected with stirring shaft.
Further, the specification of the filter screen is 200 mesh.
Further, it is provided with the second shock-absorption backing plate between the support base and housing, support base and housing pass through the Two shock-absorption backing plates are connected with each other.
Further, the filter device that crushes is internally provided with automatic heating device, and automatic heating device is with crushed The madial wall for filtering device is connected with each other.
The advantage of the invention is that:The present invention provides a kind of stem cell filter separators, and the present invention is on stirring shaft Be provided with agitating vane, lower agitating vane and screw blade, will not because shear history machinery exert oneself unevenness caused by a large amount of cells Damage, stem cell liquid can be stirred at many levels so that stirring evenly, prevent stem cell particle block sieves Net, improves the efficiency of separation and screening, and the present invention is internally provided with heating unit, heating unit and powder crushing filter device The madial wall of broken filter device is connected with each other, and the temperature crushed in filter device can be maintained 37 DEG C by heating unit, can be with The activity of stem cell is effectively kept, the present invention, which is provided with, holds the volume flow that flowmeter is used to measure stem cell solution, this hair Bright filter screen is V-arrangement stainless steel filter screen, has not only reduced the surface tension of its upper fluid, but also increases carrying liquid Surface area, volume and liquid lower floor pressure, are more advantageous under liquid flow, significantly improve the filter efficiency of stem cell, the present invention Seal rubber plug is provided with, can be effectively sealed to crushing filter device, the present invention is provided with degerming on liquid suction pipe Filter reduces the chance that pollution occurs, and improves the survival rate of stem cell.
Description of the drawings
It, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical solution of the prior art Embodiment or attached drawing needed to be used in the description of the prior art are briefly described, it should be apparent that, in describing below Attached drawing is some embodiments of the present invention, for those of ordinary skill in the art, before not making the creative labor It puts, can also be obtained according to these attached drawings other attached drawings.
Fig. 1 is the structural diagram of the present invention.
Wherein:
1- housings;2- crushes filter device;3- seal rubber plugs;
4- cell liquid inlets;5- cell liquid ascending pipes;6- solenoid valves;
7- driving motors;8- electric machine main shafts;9- drive links;
10- transmission shafts;11- speed reducers;12- stirring shafts;
13- screw blades;The upper agitating vanes of 14-;Agitating vane under 15-;
16- filter screens;17- filter membranes;18- supporting brackets;
19- support arms;The first shock-absorption backing plates of 20-;21- support plates;
The second shock-absorption backing plates of 22-;23- support bases;24- liquid suction pipes;
25- drawing liquid control valves;26- drawing liquid pumps;27- sterilizing filters;
28- flowmeters;29- separator boxs;30- catabolic enzyme ascending pipes;
31- automatic heating devices
Specific embodiment
Technical scheme is clearly and completely described below in conjunction with attached drawing, it is clear that described implementation Example is part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, ordinary skill Personnel's all other embodiments obtained without making creative work, belong to the scope of protection of the invention.
In the description of the present invention, it is necessary to explanation, term " " center ", " on ", " under ", "left", "right", " vertical ", The orientation or position relationship of the instructions such as " level ", " interior ", " outer " be based on orientation shown in the drawings or position relationship, merely to Convenient for the description present invention and simplify description rather than instruction or imply signified device or element must have specific orientation, With specific azimuth configuration and operation, therefore it is not considered as limiting the invention.In addition, term " first ", " second ", " the 3rd " is only used for description purpose, and it is not intended that instruction or hint relative importance.
Fig. 1 is the structural diagram of the present invention, a kind of stem cell filter separator as shown in Figure 1, including:Housing 1, powder Broken filter device 2, seal rubber plug 3, cell liquid inlet 4, cell liquid ascending pipe 5, solenoid valve 6, driving motor 7, electric machine main shaft 8th, drive link 9, transmission shaft 10, speed reducer 11, stirring shaft 12, screw blade 13, upper agitating vane 14, lower agitating vane 15, Filter screen 16, filter membrane 17, supporting bracket 18, support arm 19, the first shock-absorption backing plate 20, support plate 21, the second shock-absorption backing plate 22, Support base 23, liquid suction pipe 24, drawing liquid control valve 25, drawing liquid pump 26, sterilizing filter 27, flowmeter 28, separator box 29, decomposition Enzyme ascending pipe 30 and automatic heating device 31, it is characterised in that:The driving motor 7 is arranged on the one side of the housing 1, described Drive link 9 is arranged on the one side of the driving motor 7, the electric machine main shaft 8 be arranged on the driving motor 7 and drive link 9 it Between, the driving motor 7 is connected with each other by the electric machine main shaft 8 and the drive link 9, and the speed reducer 11 is arranged on described The one side of drive link 9, the transmission shaft 10 are arranged between speed reducer 11 and drive link 9, and the drive link 9 passes through transmission shaft 10 It is connected with each other with speed reducer 11, the stirring shaft 12 is arranged on the lower end of the speed reducer 11, the stirring shaft 12 and institute It states speed reducer 11 to be connected with each other, the inside for crushing filter device 2 and being arranged on the housing 1, the seal rubber plug 3 is arranged on The upper end for crushing filter device 2, the seal rubber plug 3 are connected with each other with the crushing filter device 2, the stirring shaft 12 lower end through the seal rubber plug 3 and stretch into crush filter device 2 inside, the upper agitating vane 14 with it is described under stir Blade 15 is mixed to be arranged on the stirring shaft 12, upper agitating vane 14 and lower agitating vane 15 with the stirring shaft 12 Be mutually permanently connected, the filter screen 16 is arranged on the lower end of the lower agitating vane 15, the filter screen 16 with it is described Filter device 2 is crushed to be detachably connected, the filter membrane 17 is arranged on the lower end of the filter screen 16, the filter membrane 17 It being connected with each other with the crushing filter device 2, the support arm 19 is arranged on the lower end of the crushing filter device 2, and described the One shock-absorption backing plate 20 is arranged on the lower end of the support arm 19, and first shock-absorption backing plate 20 interfixes with the support arm 19 Connection, the support plate 21 are arranged on the lower end of first shock-absorption backing plate 20, and 21 and first shock-absorption backing plate 20 of support plate is mutually It is fixedly connected, the support base 23 is arranged on the lower end of the housing 1, and the catabolic enzyme ascending pipe 30 is arranged on the crushing The upper end of filter device 2, the catabolic enzyme ascending pipe 30 are interconnected with crushing filter device 2.
Working method:The present invention provides a kind of stem cell filter separator, including:Housing crushes filter device, driving Motor, electric machine main shaft, drive link, transmission shaft, speed reducer, stirring shaft, upper agitating vane, lower agitating vane, filter screen, mistake Filter membrane, support arm, the first shock-absorption backing plate, support plate, support base, catabolic enzyme ascending pipe and automatic heating device, feature exist In:Driving motor is arranged on the one side of housing, and drive link is arranged on the one side of driving motor, and electric machine main shaft is arranged on driving motor Between drive link, driving motor is connected with each other by electric machine main shaft and drive link, and speed reducer is arranged on the one side of drive link, is passed Moving axis is arranged between speed reducer and drive link, and drive link is connected with each other by transmission shaft and speed reducer, and stirring shaft is arranged on The lower end of speed reducer, stirring shaft are connected with each other with speed reducer, crush the inside that filter device is arranged on housing, and seal rubber plug is set It puts and is crushing the upper end of filter device, seal rubber plug is connected with each other with crushing filter device, and the lower end of stirring shaft passes through sealing Rubber plug simultaneously stretches into the inside for crushing filter device, and upper agitating vane is arranged at lower agitating vane on stirring shaft, upper stirring Blade is mutually permanently connected with lower agitating vane with stirring shaft, and filter screen is arranged on the lower end of lower agitating vane, filtering Sieve is with crushing filter device to be detachably connected, and filter membrane is arranged on the lower end of filter screen, and filter membrane is with crushing filtering dress Put interconnection, support arm is arranged on the lower end for crushing filter device, and the first shock-absorption backing plate is arranged on the lower end of support arm, and first Shock-absorption backing plate is mutually permanently connected with support arm, and support plate is arranged on the lower end of the first shock-absorption backing plate, support plate and the first damping Backing plate is mutually permanently connected, and support base is arranged on the lower end of housing.The present invention be provided on stirring shaft agitating vane, Lower agitating vane and screw blade, will not because shear history machinery exert oneself it is uneven caused by a large amount of cells damage, can be multi-level Stem cell liquid is stirred so that stirring evenly, prevents stem cell particle from blocking filter screen, improves what is separated and screen Efficiency, the present invention, which is provided with, to be held flowmeter and is used to measuring the volume flow of stem cell solution, filter screen of the invention for V-arrangement not Become rusty steel filter screen, had not only reduced the surface tension of its upper fluid, but also increases under surface area, volume and the liquid of carrying liquid Lamination is strong, is more advantageous under liquid flow, significantly improves the filter efficiency of stem cell, and the present invention is provided with seal rubber plug, can be with It is effectively sealed to crushing filter device, the present invention is provided with sterilizing filter on liquid suction pipe, reduces pollution Chance, improve the survival rate of stem cell.
Finally it should be noted that:The above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations;To the greatest extent Pipe is described in detail the present invention with reference to foregoing embodiments, and the logical technical staff of this field should be understood:It is still It can modify to the technical solution recorded in foregoing embodiments and either which part or all technical characteristic are carried out Equivalent substitution;And these modifications or replacement, the essence of appropriate technical solution is not made to depart from various embodiments of the present invention technical side The scope of case.

Claims (9)

1. a kind of stem cell filter separator, including:Housing crushes filter device, driving motor, electric machine main shaft, drive link, biography Moving axis, speed reducer, stirring shaft, upper agitating vane, lower agitating vane, filter screen, filter membrane, support arm, the first beam Plate, support plate, support base and catabolic enzyme ascending pipe, it is characterised in that:The driving motor is arranged on the one side of the housing, The drive link is arranged on the one side of the driving motor, the electric machine main shaft be arranged on the driving motor and drive link it Between, the driving motor is connected with each other by the electric machine main shaft and the drive link, and the speed reducer is arranged on the transmission The one side of bar, the transmission shaft are arranged between speed reducer and drive link, and the drive link is mutual with speed reducer by transmission shaft Connection, the stirring shaft are arranged on the lower end of the speed reducer, and the stirring shaft is connected with each other with the speed reducer, described The inside that filter device is arranged on the housing is crushed, the seal rubber plug is arranged on the upper end of the crushing filter device, institute It states seal rubber plug to be connected with each other with the crushing filter device, the lower end of the stirring shaft through the seal rubber plug and is stretched into The inside of filter device is crushed, the upper agitating vane is arranged on the stirring shaft with the lower agitating vane, above stirs It mixes blade to be mutually permanently connected with the stirring shaft with lower agitating vane, the filter screen is arranged on the lower paddle The lower end of piece, for the filter screen with the crushing filter device to be detachably connected, the filter membrane is arranged on the filtering The lower end of sieve, the filter membrane are connected with each other with the crushing filter device, and the support arm is arranged on described crush and filters The lower end of device, first shock-absorption backing plate are arranged on the lower end of the support arm, first shock-absorption backing plate and the support Arm is mutually permanently connected, and the support plate is arranged on the lower end of first shock-absorption backing plate, support plate and the first shock-absorption backing plate phase It is mutually fixedly connected, the support base is arranged on the lower end of the housing, and the catabolic enzyme ascending pipe is arranged on described crushed The upper end of device is filtered, the catabolic enzyme ascending pipe is interconnected with crushing filter device.
2. a kind of stem cell filter separator according to claim 1, it is characterised in that:It is described to crush the upper of filter device End is provided with cell liquid ascending pipe, and solenoid valve is arranged on cell liquid ascending pipe, the lower end of the cell liquid ascending pipe and setting The cell liquid inlet opened up on seal rubber plug is interconnected, and the one side for crushing filter device is provided with liquid suction pipe, takes out Drawing liquid control valve, drawing liquid pump and flowmeter are provided in liquid pipe, one end of the liquid suction pipe and the crushing filter device are mutual Connection, the one side of the liquid suction pipe are provided with separator box, and the other end and the separator box of liquid suction pipe are interconnected.
3. a kind of stem cell filter separator according to claim 2, it is characterised in that:It is provided with and removes on the liquid suction pipe Bacterium filter.
4. a kind of stem cell filter separator according to claim 1, it is characterised in that:The support arm is filtered with crushing Supporting bracket is provided between device.
5. a kind of stem cell filter separator according to claim 1, it is characterised in that:The filter screen for V-arrangement not Become rusty steel filter screen.
6. a kind of stem cell filter separator according to claim 1, it is characterised in that:It is provided on the stirring shaft Screw blade, screw blade are mutually permanently connected with stirring shaft.
7. a kind of stem cell filter separator according to claim 1, it is characterised in that:The specification of the filter screen is 200 mesh.
8. a kind of stem cell filter separator according to claim 1, it is characterised in that:The support base and housing it Between be provided with the second shock-absorption backing plate, support base is connected with each other with housing by the second shock-absorption backing plate.
9. a kind of stem cell filter separator according to claim 1, it is characterised in that:It is described to crush the interior of filter device Portion is provided with automatic heating device, and automatic heating device is connected with each other with crushing the madial wall of filter device.
CN201711489074.6A 2017-12-29 2017-12-29 A kind of stem cell filter separator Pending CN108060082A (en)

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Application Number Priority Date Filing Date Title
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Cited By (5)

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Publication number Priority date Publication date Assignee Title
CN108504543A (en) * 2018-06-01 2018-09-07 湖州聚业孵化器有限公司 A kind of stem cell extractor that extraction effect is good
CN109913363A (en) * 2019-04-03 2019-06-21 何春兰 A kind of cell separation system and cell isolation method
CN111111292A (en) * 2020-01-15 2020-05-08 福州三合元生物科技有限公司 Edulcoration purification device of vitamin C glucoside
CN111257080A (en) * 2020-02-29 2020-06-09 右江民族医学院附属医院 Assay sample processing apparatus for hepatobiliary surgery
CN112940916A (en) * 2021-04-07 2021-06-11 贝姿(广州)数码科技有限公司 Separating centrifuge based on tumour screening biomembrane

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CN205420437U (en) * 2015-12-23 2016-08-03 天津滨通生物科技有限公司 Fat stem cell separation and extraction device
CN206127287U (en) * 2016-07-08 2017-04-26 成都武侯恩喜整形医疗美容门诊部有限公司 Novel be used for separating fatty stem cell device
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Cited By (7)

* Cited by examiner, † Cited by third party
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CN108504543A (en) * 2018-06-01 2018-09-07 湖州聚业孵化器有限公司 A kind of stem cell extractor that extraction effect is good
CN109913363A (en) * 2019-04-03 2019-06-21 何春兰 A kind of cell separation system and cell isolation method
CN109913363B (en) * 2019-04-03 2020-04-10 北京泰盛生物科技有限公司 Cell separation system and cell separation method
CN111111292A (en) * 2020-01-15 2020-05-08 福州三合元生物科技有限公司 Edulcoration purification device of vitamin C glucoside
CN111257080A (en) * 2020-02-29 2020-06-09 右江民族医学院附属医院 Assay sample processing apparatus for hepatobiliary surgery
CN111257080B (en) * 2020-02-29 2023-03-28 右江民族医学院附属医院 Assay specimen processing device for hepatobiliary surgery
CN112940916A (en) * 2021-04-07 2021-06-11 贝姿(广州)数码科技有限公司 Separating centrifuge based on tumour screening biomembrane

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Application publication date: 20180522