CN109913363B - Cell separation system and cell separation method - Google Patents
Cell separation system and cell separation method Download PDFInfo
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- CN109913363B CN109913363B CN201910265880.8A CN201910265880A CN109913363B CN 109913363 B CN109913363 B CN 109913363B CN 201910265880 A CN201910265880 A CN 201910265880A CN 109913363 B CN109913363 B CN 109913363B
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Abstract
The invention relates to a cell separation system and a cell separation method, which relate to the technical field of cell separation and comprise a base, a separation cylinder assembly, a screening assembly, a power assembly, a reciprocating driving mechanism, a transmission wheel assembly, a stirring assembly and an anti-blocking assembly, and have the beneficial technical effects that cells can be fully separated from mixed liquid; the filter pores with gradually reduced pore diameters are adopted to carry out gradient filtration separation on the cells, so that the cells with different sizes can be kept at a better retention rate; can effectively avoid the blockage of the screen. Separating cartridge subassembly fixed connection is on the base, screening subassembly fixed connection is between base and separating cartridge subassembly, power component fixed connection is in the upper end of separating cartridge subassembly, reciprocating drive mechanism fixed connection is in the inside upper end of separating cartridge subassembly, power component sliding fit connects on reciprocating drive mechanism, the drive wheel subassembly is provided with two, two drive wheel subassembly symmetry fixed connection are on separating cartridge subassembly.
Description
Technical Field
The invention relates to the technical field of cell separation, in particular to a cell separation system and a cell separation method.
Background
Cell sorting is the most important technical method and necessary premise in cell biology and molecular biology, and the current technology is mature and widely applied, and mainly comprises the following steps: density gradient centrifugation, immuno-density centrifugation, immunomagnetic bead method, flow technology, etc. Although these separation techniques can separate specific types of cells, they are complicated in operation, low in separation efficiency and high in cost, which limits the large-scale clinical application, and many of the existing experimental studies are based on a filter membrane and a pore size of the same size for separation, which results in the loss of cells of a specific size; when the quality of the specimen is poor, the filter membrane is easy to block, and the separation effect is influenced. The separation efficiency is low, the yield of stem cells is less, and time and labor are wasted.
Disclosure of Invention
The invention aims to provide a cell separation system and a cell separation method, which have the advantages that the cell and the mixed solution can be fully separated; the filter pores with gradually reduced pore diameters are adopted to carry out gradient filtration separation on the cells, so that the cells with different sizes can be kept at a better retention rate; can effectively avoid the blockage of the screen.
The purpose of the invention is realized by the following technical scheme:
a cell separation system comprises a base, a separation barrel assembly, a screening assembly, power assemblies, a reciprocating driving mechanism, transmission wheel assemblies, stirring assemblies and an anti-blocking assembly, wherein the separation barrel assembly is fixedly connected to the base, the screening assembly is fixedly connected between the base and the separation barrel assembly, the power assemblies are fixedly connected to the upper end of the separation barrel assembly, the reciprocating driving mechanism is fixedly connected to the upper end inside the separation barrel assembly, the power assemblies are connected to the reciprocating driving mechanism in a sliding fit manner, the number of the transmission wheel assemblies is two, the two transmission wheel assemblies are symmetrically and fixedly connected to the separation barrel assembly, the two ends of the reciprocating driving mechanism are respectively in meshing transmission connection with the two transmission wheel assemblies, the stirring assemblies are connected to the power assemblies and the separation barrel assembly, the inner ends of the two transmission wheel assemblies are in clearance fit connection with the stirring assemblies, and the anti-blocking assembly is fixedly, the anti-blocking component is in meshing transmission connection with the stirring component.
The separating cylinder assembly comprises a separating cylinder body, a screen, an arc-shaped seat plate and supporting legs; the front end of separator body is provided with row material through-hole, arranges the inboard fixed connection screen cloth of material through-hole, is provided with filtration pore I on the screen cloth, two arc bedplate of lower extreme symmetry fixed connection of separator body, two supporting legs of two arc bedplate's lower extreme difference fixed connection, four all fixed connection of supporting leg are on the base.
The screening component comprises a vertical plate, a transverse plate, a first screening plate, a second screening plate, a side plate, an L-shaped base plate, a first collecting box and a second collecting box; the two ends of the vertical plate are respectively and fixedly connected to the separating cylinder body and the base, the front end of the vertical plate is fixedly connected with two transverse plates, a first sieve plate and a second sieve plate are respectively and fixedly connected to the two transverse plates, the second sieve plate is positioned below the first sieve plate, the first sieve plate inclines towards the lower left side, the second sieve plate inclines towards the lower right side, the left end and the right end of each transverse plate are respectively and fixedly connected with a side plate, the two side plates are respectively and fixedly connected with an L-shaped base plate, the two L-shaped base plates are respectively provided with a first collecting box and a second collecting box, the first collecting box is positioned below the left end of the first sieve plate, and the second collecting box is positioned below the right end of the; the first sieve plate is provided with a filter hole II, and the second sieve plate is provided with a filter hole III; the first screen plate is positioned below the screen mesh.
The power assembly comprises a horizontal frame plate, a motor, a transmission shaft, a driving belt wheel, a rotating rod, a deflector rod and an L-shaped frame plate; horizontal frame plate fixed connection is in the upper end of cylinder body, the motor passes through motor frame fixed connection on horizontal frame plate, the output shaft of motor passes through the coupling joint transmission shaft, the middle-end of transmission shaft is rotated through taking a pedestal bearing and is connected on horizontal frame plate, driving pulley and bull stick fixed connection are respectively at the upper and lower both ends of transmission shaft, driving pulley passes through belt drive with the stirring subassembly and is connected, the outer end fixed connection driving lever of bull stick, both ends about horizontal frame plate are connected to two L type axle frame plate symmetry fixed connection.
The reciprocating driving mechanism comprises a rectangular sliding frame, a T-shaped sliding block, a trapezoidal convex strip, a fixed transverse plate, a connecting plate, a horizontal rack, a driving gear, a worm, a rod frame plate, a worm gear rotating shaft and a driving bevel gear; the front end and the rear end of the rectangular sliding frame are respectively fixedly connected with a connecting plate and a T-shaped sliding block, the T-shaped sliding block is connected to the trapezoidal raised line in a sliding fit mode, the trapezoidal raised line is fixedly connected to a fixed transverse plate, and the fixed transverse plate is fixedly connected to the upper end of the inner side of the separating cylinder body; the lower end of the connecting plate is fixedly connected with a horizontal rack which is meshed with a driving gear in a transmission way, the driving gear is fixedly connected to the middle end of a worm, the rear end of the worm is rotatably connected to a rod frame plate through a bearing with a seat, the rod frame plate is fixedly connected to a fixed transverse plate, the front end of the worm is meshed with a worm wheel in a transmission way, the worm wheel is fixedly connected to the middle end of a worm wheel rotating shaft, two ends of the worm wheel rotating shaft are respectively rotatably connected to two L-shaped shaft frame plates through bearings with seats, and the outer ends of two ends of the worm wheel rotating shaft are; the driving bevel gear is in meshed transmission connection with the driving wheel assembly; the deflector rod is connected in the rectangular sliding frame in a clearance fit manner.
The driving wheel assembly comprises a driving gear, a gear rotating shaft, a U-shaped gear seat, a driven bevel gear, a vertical rack and a shifting fork; the driving gear is fixedly connected to a gear rotating shaft, the gear rotating shaft is rotatably connected to a gear seat through a bearing with a seat, the gear seat is fixedly connected to the upper end of the inner side of the separating cylinder body, the driven bevel gear is fixedly connected to the gear rotating shaft, and the driven bevel gear is in meshing transmission connection with the driving bevel gear; the driving gear is in meshed transmission connection with the vertical rack, the vertical rack is located in the U-shaped gear seat, the outer end face of the vertical rack is attached to the inner side face of the U-shaped gear seat, the lower end of the vertical rack is fixedly connected with the shifting fork, and the shifting fork is in clearance fit connection with the stirring assembly.
The stirring assembly comprises a central shaft, a driven belt wheel, an incomplete gear, a disc, a shaft sleeve, an annular groove, a side frame plate, a blade rotating shaft and stirring blades; the two ends of the central shaft are respectively and rotatably connected with the centers of the horizontal frame plate and the inner bottom surface of the separating cylinder body through belt seat bearings, the upper end, the middle end and the lower end of the central shaft are respectively and fixedly connected with a driven belt wheel, an incomplete gear and a disc, the driven belt wheel is connected with a driving belt wheel through belt transmission, the incomplete gear is in meshing transmission connection with the anti-blocking assembly, the disc is connected in a shaft sleeve in a sliding fit manner, the front end and the rear end of the shaft sleeve are respectively provided with a through lug chute, the disc is fixedly connected with two lugs, the two lugs are respectively and slidably connected in the two lug chutes, the left end and the right end of the shaft sleeve are respectively and fixedly connected with a side frame plate, the two side frame; the upper end of the shaft sleeve is provided with an annular groove, and the inner sides of the two shifting forks are respectively connected with the two ends of the annular groove in a clearance fit manner.
The anti-blocking component comprises a stirring shaft, a shaft seat plate, a linkage gear and a stirring plate; the upper end and the lower end of the stirring shaft are respectively rotatably connected to a shaft seat plate and the bottom surface of the inner side of the separating cylinder body through bearings with seats, the shaft seat plate is fixedly connected to the inner side surface of the separating cylinder body, a linkage gear is fixedly connected to the upper end of the stirring shaft, the linkage gear is in meshing transmission connection with an incomplete gear, and the lower end of the stirring shaft is fixedly connected with a plurality of stirring plates; the stirring plate is positioned at the rear end of the screen.
The pore diameters of the filter pores I, the filter pores II and the filter pores III are sequentially decreased; the screen cloth, the first sieve plate and the second sieve plate are all made of stainless steel.
A method for cell separation using one of the above cell separation systems, the method comprising the steps of:
the method comprises the following steps: after the power assembly is electrified and started, the power assembly drives the two stirring blades on the stirring assembly to perform rotary stirring and up-and-down stirring movement;
step two: continuously pouring the mixed solution into the separation cylinder body, stirring and separating the mixed solution by the two stirring blades, and allowing the separated cells and waste liquid to flow out through the filtering holes I on the screen mesh to realize liquid inlet, separation and liquid discharge simultaneously;
step three: the separated cells fall on the first sieve plate firstly, the cells with the cell size larger than the filter hole II slide and are collected into the first collection box, the cells with the cell size smaller than the filter hole II penetrate through the filter hole II and fall on the second sieve plate, and the cells on the second sieve plate are collected into the second collection box, so that the cells with different sizes are subjected to stepped filtration, screening and separation.
The cell separation system has the beneficial effects that:
the cell separation system disclosed by the invention has the advantages that the purpose of fully separating cells from mixed liquid is realized through the mixing and stirring of rotary stirring and vertical stirring, and the incomplete cell separation is avoided; the anti-blocking component effectively avoids the problem that the cell separation efficiency is influenced by the blockage of the screen; the liquid can be fed and discharged at the same time.
The cell separation method has the beneficial effects that:
the invention relates to a cell separation method, which adopts filter pores with gradually reduced pore diameters to carry out gradient filtration separation on cells, can keep better retention rate on cells with different sizes, and can select different detection modes aiming at the cells with different sizes; the efficiency of cell separation is improved by feeding liquid, separating and discharging liquid.
Drawings
FIG. 1 is a first general structural diagram of the present invention;
FIG. 2 is a second overall view of the present invention;
FIG. 3 is a schematic view of the internal structure of the present invention;
FIG. 4 is a schematic view of the present invention in partial cross-section;
FIG. 5 is a schematic structural view of the base and separator bowl assembly;
FIG. 6 is a schematic illustration of a screen assembly;
FIG. 7 is a schematic structural view of a power assembly;
FIG. 8 is a schematic structural view of the reciprocating drive mechanism;
FIG. 9 is a schematic view of the structure of the driving wheel assembly;
FIG. 10 is a schematic view of the stirring assembly;
fig. 11 is a schematic structural view of the anti-clogging assembly.
In the figure: a base 1; a separator cylinder assembly 2; a separation cylinder body 2-1; 2-2 of a screen mesh; 2-3 of an arc-shaped seat plate; supporting legs 2-4; a screen assembly 3; a vertical plate 3-1; a transverse plate 3-2; 3-3 parts of a first sieve plate; 3-4 parts of a second sieve plate; 3-5 of a side plate; 3-6 parts of an L-shaped seat plate; a first collecting box 3-7; a second collecting box 3-8; a power assembly 4; 4-1 of a horizontal frame plate; a motor 4-2; 4-3 of a transmission shaft; 4-4 of a driving belt wheel; 4-5 of a rotating rod; 4-6 of a deflector rod; 4-7 of an L-shaped shaft bracket plate; a reciprocating drive mechanism 5; a rectangular sliding frame 5-1; 5-2 of a T-shaped sliding block; 5-3 trapezoidal convex strips; fixing a transverse plate 5-4; 5-5 of a connecting plate; 5-6 of horizontal racks; 5-7 of a driving gear; 5-8 parts of worm; 5-9 parts of a lever rack plate; 5-10 parts of worm wheel; 5-11 parts of worm wheel rotating shaft; 5-12 parts of a drive bevel gear; a driving wheel assembly 6; drive gear 6-1; a gear rotating shaft 6-2; 6-3 of a U-shaped gear seat; driven bevel gears 6-4; 6-5 of a vertical rack; 6-6 of a shifting fork; a stirring component 7; a central shaft 7-1; a driven pulley 7-2; 7-3 of an incomplete gear; 7-4 of a disc; 7-5 of a shaft sleeve; annular grooves 7-6; 7-7 of side frame plates; 7-8 of a blade rotating shaft; 7-9 parts of stirring blades; an anti-clogging element 8; a stirring shaft 8-1; a bearing plate 8-2; 8-3 of a linkage gear; and 8-4 of stirring plates.
Detailed Description
The present invention is described in further detail below with reference to the accompanying figures 1-11 and the detailed description.
The first embodiment is as follows:
the cell separation system of the present embodiment is described below with reference to fig. 1-11, and comprises a base 1, a separation cylinder assembly 2, a screening assembly 3, a power assembly 4, a reciprocating driving mechanism 5, a driving wheel assembly 6, a stirring assembly 7 and an anti-clogging assembly 8, wherein the separation cylinder assembly 2 is fixedly connected to the base 1, the screening assembly 3 is fixedly connected between the base 1 and the separation cylinder assembly 2, the power assembly 4 is fixedly connected to the upper end of the separation cylinder assembly 2, the reciprocating driving mechanism 5 is fixedly connected to the upper end inside the separation cylinder assembly 2, the power assembly 4 is connected to the reciprocating driving mechanism 5 in a sliding fit manner, two driving wheel assemblies 6 are provided, the two driving wheel assemblies 6 are symmetrically and fixedly connected to the separation cylinder assembly 2, two ends of the reciprocating driving mechanism 5 are respectively engaged and drivingly connected to the two driving wheel assemblies 6, the stirring assembly 7 is connected to the power assembly 4 and the separation, the inner ends of the two driving wheel assemblies 6 are in clearance fit connection with the stirring assembly 7, the anti-blocking assembly 8 is fixedly connected to the front end of the separating cylinder assembly 2, and the anti-blocking assembly 8 is in meshing transmission connection with the stirring assembly 7. When the invention is used, after the power component 4 is communicated with a power supply to start, the power component 4 drives the stirring component 7 to perform rotary stirring work, meanwhile, the power component 4 also drives the reciprocating driving mechanism 5 to work, the reciprocating driving mechanism 5 drives the two driving wheel components 6 to work, the two driving wheel components 6 drive the stirring component 7 to perform circulating up-and-down stirring work, mixed liquid to be separated is continuously poured into the separating cylinder component 2, the stirring component 7 performs up-and-down and rotary mixing stirring on the mixed liquid, so that cells are fully separated, the separated cells and the mixed liquid are discharged from a filter hole I at the lower end of the separating cylinder component 2, the pore diameters of a filter hole II and a filter hole III on the screening component 3 are sequentially reduced, cells with different sizes are filtered, screened and separated, the cells with different sizes are kept with good retention rate, and the stirring component 7 also drives the anti-blocking component 8 to work, prevent stifled subassembly 8 and avoid filtration pore I to block up and influence the problem of cell separation efficiency, on one side feed liquor, separate, on the other side the flowing back has improved the efficiency of cell separation.
The second embodiment is as follows:
the present embodiment will be described with reference to fig. 1 to 11, wherein the separator bowl assembly 2 comprises a separator bowl body 2-1, a screen 2-2, an arc-shaped seat plate 2-3 and support legs 2-4; the front end of the separating cylinder body 2-1 is provided with a discharging through hole, the inner side of the discharging through hole is fixedly connected with the screen 2-2, the screen 2-2 is provided with a filtering hole I, the lower end of the separating cylinder body 2-1 is symmetrically and fixedly connected with two arc-shaped seat plates 2-3, the lower ends of the two arc-shaped seat plates 2-3 are respectively and fixedly connected with two supporting legs 2-4, and the four supporting legs 2-4 are fixedly connected on the base 1. When the separating cylinder assembly 2 is used, the mixed liquid is poured into the separating cylinder body 2-1, and the separated cells and waste liquid are discharged through the filtering holes I on the screen 2-2.
The third concrete implementation mode:
referring to fig. 1-11, the screen assembly 3 includes a vertical plate 3-1, a horizontal plate 3-2, a first screen plate 3-3, a second screen plate 3-4, a side plate 3-5, an L-shaped base plate 3-6, a first collecting box 3-7 and a second collecting box 3-8; two ends of a vertical plate 3-1 are respectively fixedly connected with a separating cylinder body 2-1 and a base 1, the front end of the vertical plate 3-1 is fixedly connected with two transverse plates 3-2, a first sieve plate 3-3 and a second sieve plate 3-4 are respectively fixedly connected with the two transverse plates 3-2, the second sieve plate 3-4 is positioned below the first sieve plate 3-3, the first sieve plate 3-3 inclines towards the lower left, the second sieve plate 3-4 inclines towards the lower right, the left end and the right end of the transverse plate 3-2 are respectively fixedly connected with a side plate 3-5, an L-shaped seat plate 3-6 is respectively fixedly connected with the two side plates 3-5, a first collecting box 3-7 and a second collecting box 3-8 are respectively arranged on the two L-shaped seat plates 3-6, the first collecting box 3-7 is positioned below the left end of the first sieve plate 3-3, the second collecting box 3-8 is positioned below the right end of the second sieve plate 3-4; the first sieve plate 3-3 is provided with a filter hole II, and the second sieve plate 3-4 is provided with a filter hole III; the first screen plate 3-3 is positioned below the screen 2-2. When the screening component 3 is used, separated cells fall on the first screening plate 3-3, cells with the cell size larger than the filtering hole II slide and are collected into the first collection box 3-7, cells with the cell size smaller than the filtering hole II pass through the filtering hole II and fall on the second screening plate 3-4, and cells on the second screening plate 3-4 are collected into the second collection box 3-8, so that cells with different sizes are subjected to stepped filtration, screening and separation.
The fourth concrete implementation mode:
the present embodiment is described below with reference to fig. 1 to 11, and the power assembly 4 includes a horizontal frame plate 4-1, a motor 4-2, a transmission shaft 4-3, a driving pulley 4-4, a rotating rod 4-5, a shift lever 4-6 and an L-shaped frame plate 4-7; the horizontal frame plate 4-1 is fixedly connected to the upper end of the separating cylinder body 2-1, the motor 4-2 is fixedly connected to the horizontal frame plate 4-1 through a motor frame, an output shaft of the motor 4-2 is connected with the transmission shaft 4-3 through a coupler, the middle end of the transmission shaft 4-3 is rotatably connected to the horizontal frame plate 4-1 through a bearing with a seat, the driving pulley 4-4 and the rotating rod 4-5 are respectively and fixedly connected to the upper end and the lower end of the transmission shaft 4-3, the driving pulley 4-4 is in transmission connection with the stirring assembly 7 through a belt, the outer end of the rotating rod 4-5 is fixedly connected with the deflector rod 4-6, and the two L-shaped frame plates 4-7 are symmetrically and fixedly connected to the left. When the power assembly 4 is used, the motor 4-2 is connected with a power supply and a control switch through a lead and is started, the motor 4-2 drives the transmission shaft 4-3 to rotate around the axis of the motor 4-2, the transmission shaft 4-3 drives the driving belt wheel 4-4 and the rotating rod 4-5 to rotate, the driving belt wheel 4-4 drives the stirring assembly 7 to work, and the rotating rod 4-5 drives the deflector rod 4-6 to do circular motion.
The fifth concrete implementation mode:
the embodiment is described below with reference to fig. 1 to 11, wherein the reciprocating driving mechanism 5 includes a rectangular sliding frame 5-1, a T-shaped slider 5-2, a trapezoidal convex strip 5-3, a fixed transverse plate 5-4, a connecting plate 5-5, a horizontal rack 5-6, a driving gear 5-7, a worm 5-8, a lever frame plate 5-9, a worm wheel 5-10, a worm wheel rotating shaft 5-11 and a driving bevel gear 5-12; the front end and the rear end of the rectangular sliding frame 5-1 are respectively fixedly connected with a connecting plate 5-5 and a T-shaped slide block 5-2, the T-shaped slide block 5-2 is connected on a trapezoidal convex strip 5-3 in a sliding fit mode, the trapezoidal convex strip 5-3 is fixedly connected on a fixed transverse plate 5-4, and the fixed transverse plate 5-4 is fixedly connected at the upper end of the inner side of the separating cylinder body 2-1; the lower end of the connecting plate 5-5 is fixedly connected with a horizontal rack 5-6, the horizontal rack 5-6 is meshed with a driving gear 5-7 in a transmission way, the driving gear 5-7 is fixedly connected to the middle end of a worm 5-8, the rear end of the worm 5-8 is rotatably connected to a rod frame plate 5-9 through a bearing with a seat, the rod frame plate 5-9 is fixedly connected to a fixed transverse plate 5-4, the front end of the worm 5-8 is meshed with a worm wheel 5-10 in a transmission way, the worm wheel 5-10 is fixedly connected to the middle end of a worm wheel rotating shaft 5-11, two ends of the worm wheel rotating shaft 5-11 are respectively rotatably connected to two L-shaped shaft frame plates 4-7 through bearings with seats, and the outer ends of two ends of the worm wheel rotating shaft 5-; the driving bevel gears 5-12 are in meshed transmission connection with the transmission wheel assembly 6; the deflector rod 4-6 is connected in the rectangular sliding frame 5-1 in a clearance fit manner. When the reciprocating driving mechanism 5 is used, the deflector rod 4-6 circularly moves in the rectangular sliding frame 5-1 to drive the rectangular sliding frame 5-1 to reciprocate left and right, the T-shaped slide block 5-2 and the trapezoidal convex strip 5-3 play a role in supporting, limiting and guiding the rectangular sliding frame 5-1, the rectangular sliding frame 5-1 drives the horizontal rack 5-6 to reciprocate left and right through the connecting plate 5-5, the horizontal rack 5-6 drives the driving gear 5-7 to reciprocate around the axis of the horizontal rack, the driving gear 5-7 drives the worm 5-8 to reciprocate around the axis of the horizontal rack, the worm 5-8 drives the worm wheel 5-10 to reciprocate around the axis of the horizontal rack, the worm wheel 5-10 drives the worm wheel rotating shaft 5-11 to reciprocate around the axis of the horizontal rack, the worm wheel rotating shaft 5-7 drives the worm 5-8 to reciprocate around the axis of the horizontal rack, the two driving bevel gears 5-12 respectively drive the two driving wheel assemblies 6 to work.
The sixth specific implementation mode:
referring to fig. 1-11, the driving wheel assembly 6 includes a driving gear 6-1, a gear shaft 6-2, a U-shaped gear seat 6-3, a driven bevel gear 6-4, a vertical rack 6-5 and a shifting fork 6-6; the driving gear 6-1 is fixedly connected to the gear rotating shaft 6-2, the gear rotating shaft 6-2 is rotatably connected to the gear seat 6-3 through a bearing with a seat, the gear seat 6-3 is fixedly connected to the upper end of the inner side of the separating cylinder body 2-1, the driven bevel gear 6-4 is fixedly connected to the gear rotating shaft 6-2, and the driven bevel gear 6-4 is in meshing transmission connection with the driving bevel gear 5-12; the driving gear 6-1 is in meshed transmission connection with a vertical rack 6-5, the vertical rack 6-5 is located in a U-shaped gear seat 6-3, the outer end face of the vertical rack 6-5 is attached to the inner side face of the U-shaped gear seat 6-3, the lower end of the vertical rack 6-5 is fixedly connected with a shifting fork 6-6, and the shifting fork 6-6 is in clearance fit connection with a stirring assembly 7. When the transmission wheel assembly 6 is used, the driving bevel gear 5-12 drives the driven bevel gear 6-4 to rotate around the axis of the driven bevel gear in a reciprocating manner, the driven bevel gear 6-4 drives the gear rotating shaft 6-2 to rotate around the axis of the driven bevel gear in a reciprocating manner, the gear rotating shaft 6-2 drives the driving gear 6-1 to rotate around the axis of the driven bevel gear in a reciprocating manner, the driving gear 6-1 drives the vertical rack 6-5 to move up and down, and the vertical rack 6-5 drives the shifting fork 6-6 to move up; the outer end face of the vertical rack 6-5 is attached to the inner side face of the U-shaped gear seat 6-3, and the U-shaped gear seat 6-3 plays a limiting role on the vertical rack 6-5, so that the vertical rack 6-5 can only move up and down.
The seventh embodiment:
referring to fig. 1-11, the stirring assembly 7 includes a central shaft 7-1, a driven pulley 7-2, an incomplete gear 7-3, a circular disk 7-4, a shaft sleeve 7-5, an annular groove 7-6, a side frame plate 7-7, a blade rotating shaft 7-8 and a stirring blade 7-9; two ends of a central shaft 7-1 are respectively and rotatably connected with the center of the horizontal frame plate 4-1 and the inner bottom surface of the separating cylinder body 2-1 through bearings with seats, the upper end, the middle end and the lower end of the central shaft 7-1 are respectively and fixedly connected with a driven pulley 7-2, an incomplete gear 7-3 and a disc 7-4, the driven pulley 7-2 is connected with a driving pulley 4-4 through belt transmission, the incomplete gear 7-3 is in meshing transmission connection with an anti-blocking assembly 8, the disc 7-4 is in sliding fit connection in a shaft sleeve 7-5, the front end and the rear end of the shaft sleeve 7-5 are respectively provided with a through lug chute, the disc 7-4 is fixedly connected with two lugs which are respectively in sliding fit connection in the two lug chutes, the left end and the right end of the shaft sleeve 7-5 are respectively and fixedly connected, the two side frame plates 7-7 are respectively fixedly connected with a blade rotating shaft 7-8, and the two blade rotating shafts 7-8 are respectively fixedly connected with a stirring blade 7-9; the upper end of the shaft sleeve 7-5 is provided with an annular groove 7-6, and the inner sides of the two shifting forks 6-6 are respectively connected with the two ends of the annular groove 7-6 in a clearance fit manner. When the stirring assembly 7 is used, the driving belt wheel 4-4 drives the driven belt wheel 7-2 to rotate around the self axis in a reciprocating manner through a belt, the driven belt wheel 7-2 drives the central shaft 7-1 to rotate around the self axis in a reciprocating manner, the central shaft 7-1 drives the incomplete gear 7-3 and the disc 7-4 to rotate around the self axis in a reciprocating manner, the incomplete gear 7-3 drives the anti-blocking assembly 8 to work, the disc 7-4 drives the shaft sleeve 7-5 to rotate around the self axis in a reciprocating manner through the two convex blocks, and the shaft sleeve 7-5 drives the stirring blades 7-9 on the two blade rotating shafts 7-8 to do circular motion through the two side frame plates 7-7, so that the rotary stirring work; when the two shifting forks 6-6 move up and down, the shaft sleeves 7-5 are driven to move up and down, the bumps on the disks 7-4 can slide in the bump chutes, and the shaft sleeves 7-5 drive the two stirring blades 7-9 to stir up and down, so that the up and down and rotary mixing and stirring work of the two stirring blades 7-9 is realized.
The specific implementation mode is eight:
the embodiment is described below with reference to fig. 1 to 11, and the anti-clogging assembly 8 includes a stirring shaft 8-1, a shaft seat plate 8-2, a linkage gear 8-3 and a stirring plate 8-4; the upper end and the lower end of the stirring shaft 8-1 are respectively rotatably connected to a shaft seat plate 8-2 and the bottom surface of the inner side of the separating cylinder body 2-1 through bearings with seats, the shaft seat plate 8-2 is fixedly connected to the inner side surface of the separating cylinder body 2-1, a linkage gear 8-3 is fixedly connected to the upper end of the stirring shaft 8-1, the linkage gear 8-3 is in meshing transmission connection with an incomplete gear 7-3, and the lower end of the stirring shaft 8-1 is fixedly connected with a plurality of stirring plates 8-4; the stirring plate 8-4 is positioned at the rear end of the screen 2-2. When the anti-blocking assembly 8 is used, the incomplete gear 7-3 rotates in a reciprocating mode to drive the linkage gear 8-3 to rotate around the axis of the linkage gear 8-3, the linkage gear 8-3 drives the stirring shaft 8-1 to rotate around the axis of the linkage gear 8-1, the stirring shaft 8-1 drives the stirring plates 8-4 to rotate, and the stirring plates 8-4 stir mixed liquid near the screen 2-2, so that the problem that the cell separation efficiency is influenced due to the fact that cells block filter holes I in the screen 2-2 is solved.
The specific implementation method nine:
the following describes the present embodiment with reference to fig. 1 to 11, wherein the pore diameters of the filter pores i, ii and iii are sequentially decreased; the screen 2-2, the first screen plate 3-3 and the second screen plate 3-4 are all made of stainless steel. The pore size of filter pore III is smaller than the smallest diameter of the cells to be separated.
A method of cell separation, the method comprising the steps of:
the method comprises the following steps: after the power component 4 is powered on and started, the power component 4 drives the two stirring blades 7-9 on the stirring component 7 to perform rotary stirring and up-and-down stirring movement;
step two: continuously pouring the mixed solution into a separation cylinder body 2-1, stirring and separating the mixed solution by two stirring blades 7-9, and allowing the separated cells and waste liquid to flow out through a filtering hole I on a screen 2-2 to realize liquid inlet, separation and liquid discharge at the same time;
step three: the separated cells fall on the first sieve plate 3-3 firstly, the cells with the cell size larger than the filter hole II slide and are collected into the first collection box 3-7, the cells with the cell size smaller than the filter hole II pass through the filter hole II and fall on the second sieve plate 3-4, and the cells on the second sieve plate 3-4 are collected into the second collection box 3-8, so that the cells with different sizes are subjected to step filtration, screening and separation.
The invention relates to a cell separation system and a cell separation method, and the working principle is as follows:
when the invention is used, after the power component 4 is communicated with a power supply to start, the power component 4 drives the stirring component 7 to perform rotary stirring work, meanwhile, the power component 4 also drives the reciprocating driving mechanism 5 to work, the reciprocating driving mechanism 5 drives the two driving wheel components 6 to work, the two driving wheel components 6 drive the stirring component 7 to perform circulating up-and-down stirring work, mixed liquid to be separated is continuously poured into the separating cylinder component 2, the stirring component 7 performs up-and-down and rotary mixing stirring on the mixed liquid, so that cells are fully separated, the separated cells and the mixed liquid are discharged from a filter hole I at the lower end of the separating cylinder component 2, the pore diameters of a filter hole II and a filter hole III on the screening component 3 are sequentially reduced, cells with different sizes are filtered, screened and separated, the cells with different sizes are kept with good retention rate, and the stirring component 7 also drives the anti-blocking component 8 to work, prevent stifled subassembly 8 and avoid filtration pore I to block up and influence the problem of cell separation efficiency, on one side feed liquor, separate, on the other side the flowing back has improved the efficiency of cell separation.
It is to be understood that the above description is not intended to limit the present invention, and the present invention is not limited to the above examples, and that various changes, modifications, additions and substitutions which are within the spirit and scope of the present invention and which may be made by those skilled in the art are also within the scope of the present invention.
Claims (4)
1. The utility model provides a cell separation system, includes base (1), separator subassembly (2), screening subassembly (3), power component (4), reciprocating drive mechanism (5), drive wheel subassembly (6), stirring subassembly (7) and prevents blocking up subassembly (8), its characterized in that: the separation cylinder assembly (2) is fixedly connected on the base (1), the screening assembly (3) is fixedly connected between the base (1) and the separation cylinder assembly (2), the power assembly (4) is fixedly connected at the upper end of the separation cylinder assembly (2), the reciprocating driving mechanism (5) is fixedly connected at the upper end inside the separation cylinder assembly (2), the power assembly (4) is connected on the reciprocating driving mechanism (5) in a sliding fit manner, two transmission wheel assemblies (6) are arranged, the two transmission wheel assemblies (6) are symmetrically and fixedly connected on the separation cylinder assembly (2), two ends of the reciprocating driving mechanism (5) are respectively connected with the two transmission wheel assemblies (6) in a meshing transmission manner, the stirring assembly (7) is connected on the power assembly (4) and the separation cylinder assembly (2), the inner ends of the two transmission wheel assemblies (6) are connected with the stirring assembly (7) in a clearance fit manner, the anti-blocking component (8) is fixedly connected to the front end of the separating cylinder component (2), and the anti-blocking component (8) is in meshing transmission connection with the stirring component (7);
the separating cylinder assembly (2) comprises a separating cylinder body (2-1), a screen (2-2), an arc-shaped seat plate (2-3) and supporting legs (2-4); a discharge through hole is formed in the front end of the separating cylinder body (2-1), a screen (2-2) is fixedly connected to the inner side of the discharge through hole, a filter hole I is formed in the screen (2-2), the lower end of the separating cylinder body (2-1) is symmetrically and fixedly connected with two arc-shaped seat plates (2-3), the lower ends of the two arc-shaped seat plates (2-3) are fixedly connected with two supporting legs (2-4) respectively, and the four supporting legs (2-4) are all fixedly connected to the base (1);
the power assembly (4) comprises a horizontal frame plate (4-1), a motor (4-2), a transmission shaft (4-3), a driving pulley (4-4), a rotating rod (4-5), a deflector rod (4-6) and an L-shaped frame plate (4-7); the horizontal frame plate (4-1) is fixedly connected to the upper end of the separating cylinder body (2-1), the motor (4-2) is fixedly connected to the horizontal frame plate (4-1) through a motor frame, an output shaft of the motor (4-2) is connected with the transmission shaft (4-3) through a coupler, the middle end of the transmission shaft (4-3) is rotatably connected to the horizontal frame plate (4-1) through a bearing with a seat, the driving pulley (4-4) and the rotating rod (4-5) are respectively and fixedly connected to the upper end and the lower end of the transmission shaft (4-3), the driving pulley (4-4) is in transmission connection with the stirring assembly (7) through a belt, the outer end of the rotating rod (4-5) is fixedly connected with the deflector rod (4-6), and the two L-shaped frame plates (4-7) are symmetrically and fixedly connected to the left end and the right;
the reciprocating driving mechanism (5) comprises a rectangular sliding frame (5-1), a T-shaped sliding block (5-2), a trapezoidal convex strip (5-3), a fixed transverse plate (5-4), a connecting plate (5-5), a horizontal rack (5-6), a driving gear (5-7), a worm (5-8), a rod frame plate (5-9), a worm gear (5-10), a worm gear rotating shaft (5-11) and a driving bevel gear (5-12); the front end and the rear end of the rectangular sliding frame (5-1) are respectively fixedly connected with a connecting plate (5-5) and a T-shaped sliding block (5-2), the T-shaped sliding block (5-2) is connected on a trapezoidal raised line (5-3) in a sliding fit mode, the trapezoidal raised line (5-3) is fixedly connected on a fixed transverse plate (5-4), and the fixed transverse plate (5-4) is fixedly connected at the upper end of the inner side of the separating cylinder body (2-1); the lower end of the connecting plate (5-5) is fixedly connected with a horizontal rack (5-6), the horizontal rack (5-6) is in meshed transmission connection with a driving gear (5-7), the driving gear (5-7) is fixedly connected to the middle end of a worm (5-8), the rear end of the worm (5-8) is rotatably connected to a rod frame plate (5-9) through a bearing with a seat, the rod frame plate (5-9) is fixedly connected to a fixed transverse plate (5-4), the front end of the worm (5-8) is in meshed transmission connection with a worm wheel (5-10), the worm wheel (5-10) is fixedly connected to the middle end of a worm wheel rotating shaft (5-11), two ends of the worm wheel rotating shaft (5-11) are respectively rotatably connected to two L-shaped shaft frame plates (4-7) through bearings with a seat, and the outer ends of two ends of the worm wheel (5-11) are respectively and fixedly connected with a; the driving bevel gears (5-12) are in meshed transmission connection with the transmission wheel assembly (6); the deflector rod (4-6) is connected in the rectangular sliding frame (5-1) in a clearance fit manner;
the transmission wheel assembly (6) comprises a driving gear (6-1), a gear rotating shaft (6-2), a U-shaped gear seat (6-3), a driven bevel gear (6-4), a vertical rack (6-5) and a shifting fork (6-6); the driving gear (6-1) is fixedly connected to the gear rotating shaft (6-2), the gear rotating shaft (6-2) is rotatably connected to the gear seat (6-3) through a bearing with a seat, the gear seat (6-3) is fixedly connected to the upper end of the inner side of the separating cylinder body (2-1), the driven bevel gear (6-4) is fixedly connected to the gear rotating shaft (6-2), and the driven bevel gear (6-4) is in meshing transmission connection with the driving bevel gear (5-12); the driving gear (6-1) is in meshed transmission connection with a vertical rack (6-5), the vertical rack (6-5) is located in a U-shaped gear seat (6-3), the outer end face of the vertical rack (6-5) is attached to the inner side face of the U-shaped gear seat (6-3), the lower end of the vertical rack (6-5) is fixedly connected with a shifting fork (6-6), and the shifting fork (6-6) is in clearance fit connection with a stirring assembly (7);
the stirring assembly (7) comprises a central shaft (7-1), a driven belt wheel (7-2), an incomplete gear (7-3), a circular disc (7-4), a shaft sleeve (7-5), an annular groove (7-6), a side frame plate (7-7), a blade rotating shaft (7-8) and stirring blades (7-9); two ends of a central shaft (7-1) are respectively and rotatably connected with the center of the inner bottom surface of a horizontal frame plate (4-1) and a separating cylinder body (2-1) through belt seat bearings, the upper end, the middle end and the lower end of the central shaft (7-1) are respectively and fixedly connected with a driven belt pulley (7-2), an incomplete gear (7-3) and a disc (7-4), the driven belt pulley (7-2) is connected with a driving belt pulley (4-4) through belt transmission, the incomplete gear (7-3) is in meshing transmission connection with an anti-blocking component (8), the disc (7-4) is in sliding fit connection in a shaft sleeve (7-5), the front end and the rear end of the shaft sleeve (7-5) are respectively provided with a penetrating bump chute, the disc (7-4) is fixedly connected with two bumps, the two bumps are respectively in sliding fit connection in the two bump, the left end and the right end of the shaft sleeve (7-5) are respectively fixedly connected with a side frame plate (7-7), two blade rotating shafts (7-8) are respectively and fixedly connected with the two side frame plates (7-7), and two stirring blades (7-9) are respectively and fixedly connected with the two blade rotating shafts (7-8); the upper end of the shaft sleeve (7-5) is provided with an annular groove (7-6), and the inner sides of the two shifting forks (6-6) are respectively connected with the two ends of the annular groove (7-6) in a clearance fit manner;
the anti-blocking component (8) comprises a stirring shaft (8-1), a shaft seat plate (8-2), a linkage gear (8-3) and a stirring plate (8-4); the upper end and the lower end of the stirring shaft (8-1) are respectively rotatably connected to a shaft seat plate (8-2) and the bottom surface of the inner side of the separating cylinder body (2-1) through bearings with seats, the shaft seat plate (8-2) is fixedly connected to the inner side surface of the separating cylinder body (2-1), a linkage gear (8-3) is fixedly connected to the upper end of the stirring shaft (8-1), the linkage gear (8-3) is in meshing transmission connection with an incomplete gear (7-3), and the lower end of the stirring shaft (8-1) is fixedly connected with a plurality of stirring plates (8-4); the stirring plate (8-4) is positioned at the rear end of the screen (2-2).
2. A cell separation system according to claim 1, wherein: the screening component (3) comprises a vertical plate (3-1), a transverse plate (3-2), a first screening plate (3-3), a second screening plate (3-4), a side plate (3-5), an L-shaped seat plate (3-6), a first collecting box (3-7) and a second collecting box (3-8); two ends of a vertical plate (3-1) are respectively fixedly connected with a separating cylinder body (2-1) and a base (1), the front end of the vertical plate (3-1) is fixedly connected with two transverse plates (3-2), a first sieve plate (3-3) and a second sieve plate (3-4) are respectively fixedly connected with the two transverse plates (3-2), the second sieve plate (3-4) is positioned below the first sieve plate (3-3), the first sieve plate (3-3) inclines towards the left lower side, the second sieve plate (3-4) inclines towards the right lower side, the left end and the right end of the transverse plate (3-2) are respectively fixedly connected with a side plate (3-5), the two side plates (3-5) are respectively fixedly connected with an L-shaped seat plate (3-6), the two L-shaped seat plates (3-6) are respectively provided with a first collecting box (3-7) and a second collecting box (3-8), the first collecting box (3-7) is positioned below the left end of the first sieve plate (3-3), and the second collecting box (3-8) is positioned below the right end of the second sieve plate (3-4); the first sieve plate (3-3) is provided with a filter hole II, and the second sieve plate (3-4) is provided with a filter hole III; the first sieve plate (3-3) is positioned below the sieve (2-2).
3. A cell separation system according to claim 2, wherein: the pore diameters of the filter pores I, the filter pores II and the filter pores III are sequentially decreased; the screen (2-2), the first sieve plate (3-3) and the second sieve plate (3-4) are all made of stainless steel.
4. A method of cell separation using a cell separation system according to claim 3, wherein: the method comprises the following steps:
the method comprises the following steps: after the power component (4) is electrified and opened, the power component (4) drives two stirring blades (7-9) on the stirring component (7) to perform rotary stirring and up-and-down stirring movement;
step two: continuously pouring the mixed solution into a separation cylinder body (2-1), stirring and separating the mixed solution by two stirring blades (7-9), and allowing the separated cells and waste liquid to flow out through a filtering hole I on a screen (2-2) to realize liquid inlet, separation and liquid discharge at the same time;
step three: the separated cells fall on the first sieve plate (3-3) firstly, the cells with the cell size larger than the filter hole II slide and are collected into the first collection box (3-7), the cells with the cell size smaller than the filter hole II pass through the filter hole II and fall on the second sieve plate (3-4), and the cells on the second sieve plate (3-4) are collected into the second collection box (3-8), so that the cells with different sizes are subjected to stepped filtration, screening and separation.
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| CN111073796A (en) * | 2020-03-04 | 2020-04-28 | 山东第一医科大学(山东省医学科学院) | Special separator of circulation tumor cell |
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| CN108060082A (en) * | 2017-12-29 | 2018-05-22 | 天津普瑞赛尔生物科技有限公司 | A kind of stem cell filter separator |
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