CN109913363A - A kind of cell separation system and cell isolation method - Google Patents
A kind of cell separation system and cell isolation method Download PDFInfo
- Publication number
- CN109913363A CN109913363A CN201910265880.8A CN201910265880A CN109913363A CN 109913363 A CN109913363 A CN 109913363A CN 201910265880 A CN201910265880 A CN 201910265880A CN 109913363 A CN109913363 A CN 109913363A
- Authority
- CN
- China
- Prior art keywords
- fixedly connected
- plate
- gear
- cell
- sieve
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000926 separation method Methods 0.000 title claims abstract description 83
- 238000002955 isolation Methods 0.000 title abstract description 6
- 238000002156 mixing Methods 0.000 claims abstract description 39
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims abstract description 30
- 230000000712 assembly Effects 0.000 claims abstract description 26
- 238000000429 assembly Methods 0.000 claims abstract description 26
- 230000005540 biological transmission Effects 0.000 claims description 44
- 238000003756 stirring Methods 0.000 claims description 32
- 238000005096 rolling process Methods 0.000 claims description 17
- 238000000034 method Methods 0.000 claims description 13
- 244000309464 bull Species 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- 239000002699 waste material Substances 0.000 claims description 4
- 230000008878 coupling Effects 0.000 claims description 3
- 238000010168 coupling process Methods 0.000 claims description 3
- 238000005859 coupling reaction Methods 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 239000011259 mixed solution Substances 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 239000011295 pitch Substances 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
- 238000001914 filtration Methods 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 70
- 238000010586 diagram Methods 0.000 description 11
- 230000000903 blocking effect Effects 0.000 description 3
- 238000013519 translation Methods 0.000 description 3
- 238000010626 work up procedure Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000002146 bilateral effect Effects 0.000 description 2
- 238000000432 density-gradient centrifugation Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 241000237858 Gastropoda Species 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 238000011072 cell harvest Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Landscapes
- Filtration Of Liquid (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A kind of cell separation system of the present invention and cell isolation method, it is related to technical field of cell separation, including pedestal, separation cartridge module, Screen assemblies, Power Component, reciprocating drive mechanism, driving pulley assembly, mixing component and anti-blocking plug assembly, advantageous effects are that the present invention can be such that cell is sufficiently separated with mixed liquor；Gradient filtration separation is carried out to cell using the filter opening that aperture is gradually reduced, preferable rejection can be kept to various sizes of cell；Effectively sieve can be avoided to block.Separation cartridge module is fixedly connected on pedestal, Screen assemblies are fixedly connected between pedestal and separation cartridge module, Power Component is fixedly connected on the upper end of separation cartridge module, reciprocating drive mechanism is fixedly connected on the upper end of cylinder component internal, Power Component is slidably connected on reciprocating drive mechanism, there are two driving pulley assembly settings, and two driving pulley assemblies are symmetrically fixedly connected on separation cartridge module.
Description
Technical field
The present invention relates to technical field of cell separation, are in particular a kind of cell separation system and cell separation side
Method.
Background technique
Cell sorting is cell biology, the most important technical method of molecular biology and prerequisite, at present technology ratio
More mature and application is wide, mainly has: density-gradient centrifugation method, immune Density Gradient Centrifugation, immunomagnetic beads method and streaming technology
Deng.Although these isolation technics can be realized the separation of specific cell type, but complicated for operation, low separation efficiency, at high cost, limit
Its large-scale application clinically is made, and the existing research much tested is all based on a filter membrane and same size
Aperture is separated, and will lead to the loss of specific dimensions cell in this way；When specimen quality is poor, it is stifled to be also easy to generation filter membrane
Plug, influences separating effect.Its low separation efficiency, stem cell harvest yield is few, time-consuming and laborious.
Summary of the invention
The object of the present invention is to provide a kind of cell separation system and cell isolation method, its advantages are energy of the present invention
Enough it is sufficiently separated cell with mixed liquor；Gradient filtration separation is carried out to cell using the filter opening that aperture is gradually reduced, it can be right
Various sizes of cell keeps preferable rejection；Effectively sieve can be avoided to block.
The purpose of the present invention is achieved through the following technical solutions:
A kind of cell separation system, including pedestal, separation cartridge module, Screen assemblies, Power Component, reciprocating drive mechanism,
Driving pulley assembly, mixing component and anti-blocking plug assembly, the separation cartridge module are fixedly connected on pedestal, and Screen assemblies are fixed
It is connected between pedestal and separation cartridge module, Power Component is fixedly connected on the upper end of separation cartridge module, and reciprocating drive mechanism is solid
Surely it is connected to the upper end of cylinder component internal, Power Component is slidably connected the driving pulley assembly on reciprocating drive mechanism
There are two settings, two driving pulley assemblies be symmetrically fixedly connected on separation cartridge module on, the both ends of reciprocating drive mechanism respectively with
Two driving pulley assemblies are connected by meshing transmission, and mixing component is connected on Power Component and separation cartridge module, two driving wheel groups
The inner end of part is all connect with mixing component clearance fit, and anti-blocking plug assembly is fixedly connected on the front end of separation cartridge module, anti-clogging
Component is connected by meshing transmission with mixing component.
The separation cartridge module includes bowl body, sieve, arc seat board and supporting leg；The front end of bowl body
It is provided with discharge through-hole, sieve is fixedly connected on the inside of discharge through-hole, filter opening I, the lower end of bowl body are provided on sieve
Two arc seat boards symmetrically are fixedly connected with, the lower end of two arc seat boards is respectively fixedly connected with two supporting legs, four supporting legs
All it is fixedly connected on pedestal.
The Screen assemblies include riser, transverse slat, the first sieve plate, the second sieve plate, side plate, L-type seat board, the first collection box
With the second collection box；The both ends of riser are respectively fixedly connected in bowl body and pedestal, and the front end of riser is fixedly connected with two
Transverse slat, the first sieve plate and the second sieve plate are respectively fixedly connected on two transverse slats, and the second sieve plate is located at the lower section of the first sieve plate, the
Inclined downward, the second sieve plate tilt one sieve plate to the right to the left, and the left and right ends of transverse slat are respectively fixedly connected with a side plate, and two
It is respectively fixedly connected with a L-type seat board on a side plate, the first collection box and the second collection box is respectively set on two L-type seat boards,
First collection box is located at the lower section of the first sieve plate left end, and the second collection box is located at the lower section of the second sieve plate right end；First sieve
It is provided with filter opening II on plate, filter opening III is provided on the second sieve plate；First sieve plate is located at the lower section of sieve.
The Power Component includes horizontal frame plate, motor, transmission shaft, driving pulley, bull stick, driving lever and L-type pedestal plate；
Horizontal frame plate is fixedly connected on the upper end of bowl body, and motor is fixedly connected on horizontal frame plate by motor rack, motor
Output shaft connects transmission shaft by shaft coupling, and the middle-end of transmission shaft is rotatably connected on horizontal frame plate by rolling bearing units, actively
Belt wheel and bull stick are respectively fixedly connected with the upper and lower ends in transmission shaft, driving pulley and mixing component and are sequentially connected by belt,
The outer end of bull stick is fixedly connected with driving lever, and two L-type pedestal plates are symmetrically fixedly connected on the left and right ends of horizontal frame plate.
The reciprocating drive mechanism includes rectangle sliding sash, T-type sliding block, trapezoidal raised line, fixed transverse slat, connecting plate, level
Rack gear, driving gear, worm screw, bridge plate, worm gear, worm wheel shaft and drive bevel gear；The rear and front end of rectangle sliding sash is solid respectively
Surely connecting plate and T-type sliding block are connected, T-type sliding block is slidably connected on trapezoidal raised line, and trapezoidal raised line is fixedly connected on fixation
On transverse slat, fixed transverse slat is fixedly connected on the upper end on the inside of bowl body；The lower end of the connecting plate is fixedly connected with horizontal teeth
Item, horizontal rack are connected by meshing transmission driving gear, and driving gear is fixedly connected on the middle-end of worm screw, and the rear end of worm screw passes through band
Seat bearing is rotatably connected on bridge plate, and bridge plate is fixedly connected on fixed transverse slat, and the front end of worm screw is connected by meshing transmission snail
Wheel, worm gear are fixedly connected on the middle-end of worm wheel shaft, and the both ends of worm wheel shaft pass through rolling bearing units respectively and are rotatably connected on two L
On profile shaft frame plate, the outer end at worm wheel shaft both ends is respectively fixedly connected with a drive bevel gear；The drive bevel gear and transmission
Wheel assembly is connected by meshing transmission；Driving lever clearance fit is connected in rectangle sliding sash.
The driving pulley assembly include driving gear, rotary gear shaft, U-shaped pinion stand, driven wheel of differential, vertical rack gear and
Shift fork；Driving gear is fixedly connected on rotary gear shaft, and rotary gear shaft is rotatably connected on pinion stand by rolling bearing units, gear
Seat is fixedly connected on upper end on the inside of bowl body, and driven wheel of differential is fixedly connected on rotary gear shaft, driven wheel of differential with
Drive bevel gear is connected by meshing transmission；The driving gear is connected by meshing transmission with vertical rack gear, and vertical rack gear is located at U-shaped gear
In seat, the outer end face of vertical rack gear is bonded with the medial surface of U-shaped pinion stand, and the lower end of vertical rack gear is fixedly connected with shift fork, shift fork
Clearance fit is connected on mixing component.
The mixing component include central axis, driven pulley, partial gear, disk, axle sleeve, annular groove, lateral shelf plate,
Blade rotor and stirring blade；The both ends of central axis pass through rolling bearing units respectively and are rotatably connected on horizontal frame plate and bowl body
The center of inside bottom surface, upper end, middle-end and the lower end of central axis are respectively fixedly connected with driven pulley, partial gear and disk,
Driven pulley connects driving pulley by belt V belt translation, and partial gear is connected by meshing transmission with anti-blocking plug assembly, and disk is sliding
Dynamic to be cooperatively connected in axle sleeve, a perforative convex block sliding slot is respectively set in the rear and front end of axle sleeve, is fixedly connected with two on disk
A convex block, two convex blocks are slidably connected respectively in two convex block sliding slots, and the left and right ends of axle sleeve are respectively fixedly connected with one
A lateral shelf plate is respectively fixedly connected with a blade rotor on two lateral shelf plates, is respectively fixedly connected with one on two blade rotors
Stirring blade；The upper end of axle sleeve is provided with annular groove, and clearance fit is connected to the both ends of annular groove respectively for the inside of two shift forks.
The anti-blocking plug assembly includes agitating shaft, axle bed plate, linkage gear and agitating plate；The upper and lower ends of agitating shaft point
It is not rotatably connected on the bottom surface on the inside of axle bed plate and bowl body by rolling bearing units, axle bed plate is fixedly connected on cylinder
On ontology medial surface, linkage gear is fixedly connected on the upper end of agitating shaft, and linkage gear is connected by meshing transmission with partial gear,
The lower end of agitating shaft is fixedly connected with muti-piece agitating plate；The agitating plate is located at the rear end of sieve.
Successively successively decrease in the aperture of the filter opening I, filter opening II and filter opening III；The material of sieve, the first sieve plate and the second sieve plate
Matter is all stainless steel.
Using a kind of above-mentioned method that cell separation system carries out cell separation, method includes the following steps:
Step 1: Power Component is powered after unlatching, and Power Component drives two stirring blades on mixing component to be revolved
Turn stirring and upper and lower stirring movement；
Step 2: mixed liquor is persistently poured into bowl body, and two stirring blades are sirred and separated mixed solution, point
The cell and waste liquid separated out is flowed out by the filter opening I on sieve, realizes side feed liquor, side separation, side drain；
Step 3: the cell isolated first is fallen on the first sieve plate, and the cell that cell size is greater than filter opening II, which slides, to be collected
Into in the first collection box, the cell that cell size is less than filter opening II passes through filter opening II and falls on the second sieve plate, on the second sieve plate
Cell is collected into the second collection box, and realization carries out steps filtering, screening and separation to various sizes of cell.
A kind of cell separation system of the invention has the beneficial effect that
A kind of cell separation system of the present invention, the present invention by Stirring and the mixing stirred up and down make cell and
The purpose that mixed liquor is sufficiently separated avoids cell separation incomplete；Anti-blocking plug assembly effectively avoids sieve from blocking and influence cell
The problem of separative efficiency；One side feed liquor, one side separating liquor drainage may be implemented.
A kind of method of cell separation of the present invention has the beneficial effect that
A kind of method of cell separation of the present invention, the present invention carry out gradient mistake to cell using the filter opening that aperture is gradually reduced
Filter separation can keep preferable rejection to various sizes of cell, can select different inspections for various sizes of cell
Survey mode；On one side feed liquor, on one side separate, while drain improve cell separation efficiency.
Detailed description of the invention
Fig. 1 is overall structure diagram one of the invention；
Fig. 2 is overall structure diagram two of the invention；
Fig. 3 is interior section structural schematic diagram of the invention；
Fig. 4 is partial cross-sectional structural diagram of the invention；
Fig. 5 is pedestal and the structural schematic diagram for separating cartridge module；
Fig. 6 is the structural schematic diagram of Screen assemblies；
Fig. 7 is the structural schematic diagram of Power Component；
Fig. 8 is the structural schematic diagram of reciprocating drive mechanism；
Fig. 9 is the structural schematic diagram of driving pulley assembly；
Figure 10 is the structural schematic diagram of mixing component；
Figure 11 is the structural schematic diagram of anti-blocking plug assembly.
In figure: pedestal 1；Separate cartridge module 2；Bowl body 2-1；Sieve 2-2；Arc seat board 2-3；Supporting leg 2-4；Sieve
Subassembly 3；Riser 3-1；Transverse slat 3-2；First sieve plate 3-3；Second sieve plate 3-4；Side plate 3-5；L-type seat board 3-6；First collection box
3-7；Second collection box 3-8；Power Component 4；Horizontal frame plate 4-1；Motor 4-2；Transmission shaft 4-3；Driving pulley 4-4；Bull stick 4-
5；Driving lever 4-6；L-type pedestal plate 4-7；Reciprocating drive mechanism 5；Rectangle sliding sash 5-1；T-type sliding block 5-2；Trapezoidal raised line 5-3；It is fixed
Transverse slat 5-4；Connecting plate 5-5；Horizontal rack 5-6；Driving gear 5-7；Worm screw 5-8；Bridge plate 5-9；Worm gear 5-10；Worm wheel shaft
5-11；Drive bevel gear 5-12；Driving pulley assembly 6；Drive gear 6-1；Rotary gear shaft 6-2；U-shaped pinion stand 6-3；From mantle tooth
Take turns 6-4；Vertical rack gear 6-5；Shift fork 6-6；Mixing component 7；Central axis 7-1；Driven pulley 7-2；Partial gear 7-3；Disk
7-4；Axle sleeve 7-5；Annular groove 7-6；Lateral shelf plate 7-7；Blade rotor 7-8；Stirring blade 7-9；Anti-blocking plug assembly 8；Agitating shaft 8-
1；Axle bed plate 8-2；Linkage gear 8-3；Agitating plate 8-4.
Specific embodiment
The present invention will be further described in detail for 1-11 and specific embodiment with reference to the accompanying drawing.
Specific embodiment 1:
Illustrate present embodiment below with reference to Fig. 1-11, a kind of cell separation system, including pedestal 1, separation cartridge module 2,
Screen assemblies 3, Power Component 4, reciprocating drive mechanism 5, driving pulley assembly 6, mixing component 7 and anti-blocking plug assembly 8, point
It is fixedly connected on pedestal 1 from cartridge module 2, Screen assemblies 3 are fixedly connected between pedestal 1 and separation cartridge module 2, Power Component
4 are fixedly connected on the upper end of separation cartridge module 2, and reciprocating drive mechanism 5 is fixedly connected on the upper end inside separation cartridge module 2, moves
Power component 4 is slidably connected on reciprocating drive mechanism 5, and there are two the settings of driving pulley assembly 6, and two driving pulley assemblies 6 are right
Title is fixedly connected on separation cartridge module 2, and the both ends of reciprocating drive mechanism 5 connect with two 6 engaged transmissions of driving pulley assembly respectively
Connect, mixing component 7 be connected to Power Component 4 and separation cartridge module 2 on, the inner end of two driving pulley assemblies 6 all with mixing component 7
Clearance fit connection, anti-blocking plug assembly 8 are fixedly connected on the front end of separation cartridge module 2, and anti-blocking plug assembly 8 is nibbled with mixing component 7
Close transmission connection.In the use of the present invention, by Power Component 4 be connected to power initiation after, Power Component 4 drive mixing component 7 into
The work of row Stirring, while Power Component 4 also drives reciprocating drive mechanism 5 to work, reciprocating drive mechanism 5 drives two transmissions
Wheel assembly 6 works, the stirring work up and down that two driving pulley assemblies 6 drive mixing component 7 to be recycled, by mixing to be separated
Liquid is persistently poured into separation cartridge module 2, and mixing component 7 be mixed with rotation up and down to mixed liquor, divides cell sufficiently
From the cell and mixed liquor isolated are discharged from the filter opening I of separation 2 lower end of cartridge module, filter opening II and filter in Screen assemblies 3
The aperture in hole III is successively successively decreased, and is filtered, sieves, is separated to various sizes of cell, to various sizes of cell keep compared with
Good rejection, mixing component 7 also drive anti-blocking plug assembly 8 to work, and anti-blocking plug assembly 8 avoids filter opening I from blocking and influence cell
The problem of separative efficiency, on one side feed liquor, on one side separate, while drain improve cell separation efficiency.
Specific embodiment 2:
Illustrate present embodiment below with reference to Fig. 1-11, the separation cartridge module 2 includes bowl body 2-1, sieve
2-2, arc seat board 2-3 and supporting leg 2-4；The front end of bowl body 2-1 is provided with discharge through-hole, and the inside of discharge through-hole is solid
Surely sieve 2-2 is connected, is provided with filter opening I on sieve 2-2, the lower end of bowl body 2-1 is symmetrically fixedly connected with two arc-shaped seats
Plate 2-3, the lower end of two arc seat board 2-3 are respectively fixedly connected with two supporting leg 2-4, and four supporting leg 2-4 are all fixedly connected
On pedestal 1.When in use, mixed liquor is poured into bowl body 2-1 for the separation cartridge module 2, the cell after separation
And waste liquid is discharged by the filter opening I on sieve 2-2.
Specific embodiment 3:
Illustrate that present embodiment, the Screen assemblies 3 include riser 3-1, transverse slat 3-2, first below with reference to Fig. 1-11
Sieve plate 3-3, the second sieve plate 3-4, side plate 3-5, L-type seat board 3-6, the first collection box 3-7 and the second collection box 3-8；Riser 3-1's
Both ends are respectively fixedly connected in bowl body 2-1 and pedestal 1, and the front end of riser 3-1 is fixedly connected with two transverse slat 3-2, and first
Sieve plate 3-3 and the second sieve plate 3-4 are respectively fixedly connected on two transverse slat 3-2, and the second sieve plate 3-4 is located at the first sieve plate 3-3's
Lower section, inclined downward, the second sieve plate 3-4 are tilted the first sieve plate 3-3 to the right to the left, and the left and right ends of transverse slat 3-2 are solid respectively
Surely it connects to be respectively fixedly connected on side plate a 3-5, two side plate 3-5 on L-type seat board a 3-6, two L-type seat board 3-6 and divide
First collection box 3-7 is not set and the second collection box 3-8, the first collection box 3-7 is located at the lower section of the first left end sieve plate 3-3, the
Two collection box 3-8 are located at the lower section of the second sieve plate 3-4 right end；Filter opening II, the second sieve plate 3- are provided on the first sieve plate 3-3
Filter opening III is provided on 4；The first sieve plate 3-3 is located at the lower section of sieve 2-2.The Screen assemblies 3 when in use, separate
Cell out is first fallen on the first sieve plate 3-3, and the cell landing that cell size is greater than filter opening II is collected into the first collection box 3-7
Interior, the cell that cell size is less than filter opening II passes through filter opening II and falls on the second sieve plate 3-4, and the cell on the second sieve plate 3-4 is received
Collect into the second collection box 3-8, realization carries out steps filtering, screening and separation to various sizes of cell.
Specific embodiment 4:
Illustrate present embodiment below with reference to Fig. 1-11, the Power Component 4 include horizontal frame plate 4-1, motor 4-2,
Transmission shaft 4-3, driving pulley 4-4, bull stick 4-5, driving lever 4-6 and L-type pedestal plate 4-7；Horizontal frame plate 4-1 is fixedly connected on separation
The upper end of cylinder ontology 2-1, motor 4-2 are fixedly connected on horizontal frame plate 4-1 by motor rack, and the output shaft of motor 4-2 passes through
Shaft coupling connects transmission shaft 4-3, and the middle-end of transmission shaft 4-3 is rotatably connected on horizontal frame plate 4-1 by rolling bearing units, main belt
Wheel 4-4 and bull stick 4-5 is respectively fixedly connected with the upper and lower ends in transmission shaft 4-3, and driving pulley 4-4 and mixing component 7 pass through skin
V belt translation connection, the outer end of bull stick 4-5 are fixedly connected with driving lever 4-6, and two L-type pedestal plate 4-7 are symmetrically fixedly connected on level frame
The left and right ends of plate 4-1.The Power Component 4 passes through wire connecting power and control switch simultaneously when in use, by motor 4-2
It opens, motor 4-2 drives transmission shaft 4-3 around own axis, and transmission shaft 4-3 drives driving pulley 4-4 and bull stick 4-5 to turn
Dynamic, driving pulley 4-4 drives mixing component 7 to work, and bull stick 4-5 drives driving lever 4-6 to move in a circle.
Specific embodiment 5:
Illustrate present embodiment below with reference to Fig. 1-11, the reciprocating drive mechanism 5 is sliding including rectangle sliding sash 5-1, T-type
Block 5-2, trapezoidal raised line 5-3, fixed transverse slat 5-4, connecting plate 5-5, horizontal rack 5-6, driving gear 5-7, worm screw 5-8, bridge
Plate 5-9, worm gear 5-10, worm wheel shaft 5-11 and drive bevel gear 5-12；The rear and front end of rectangle sliding sash 5-1 is respectively fixedly connected with
On trapezoidal raised line 5-3, trapezoidal raised line 5-3 is fixed to be connected for connecting plate 5-5 and T-type sliding block 5-2, T-type sliding block 5-2 sliding connection
It connects on fixed transverse slat 5-4, fixed transverse slat 5-4 is fixedly connected on the upper end on the inside of bowl body 2-1；The connecting plate 5-5
Lower end be fixedly connected with horizontal rack 5-6, horizontal rack 5-6 is connected by meshing transmission driving gear 5-7, and driving gear 5-7 is fixed
It is connected to the middle-end of worm screw 5-8, the rear end of worm screw 5-8 is rotatably connected on bridge plate 5-9 by rolling bearing units, bridge plate 5-9
It is fixedly connected on fixed transverse slat 5-4, the front end of worm screw 5-8 is connected by meshing transmission worm gear 5-10, and worm gear 5-10 is fixedly connected on
The middle-end of worm wheel shaft 5-11, the both ends of worm wheel shaft 5-11 pass through rolling bearing units respectively and are rotatably connected on two L-type pedestal plates
On 4-7, the outer end at the both ends worm wheel shaft 5-11 is respectively fixedly connected with a drive bevel gear 5-12；The drive bevel gear 5-12
It is connected by meshing transmission with driving pulley assembly 6；Driving lever 4-6 clearance fit is connected in rectangle sliding sash 5-1.The reciprocal driving machine
When in use, driving lever 4-6 moves in a circle in rectangle sliding sash 5-1 drives rectangle sliding sash 5-1 bilateral reciprocation to structure 5, and T-type is sliding
Block 5-2 and trapezoidal raised line 5-3 plays the role of support limit guiding to rectangle sliding sash 5-1, and rectangle sliding sash 5-1 passes through connecting plate
5-5 drives horizontal rack 5-6 bilateral reciprocation, and horizontal rack 5-6 drives driving gear 5-7 around own axes reciprocating rotation,
Driving gear 5-7 drives worm screw 5-8 around own axes reciprocating rotation, and worm screw 5-8 drives worm gear 5-10 around own axes reciprocating rotary
Dynamic, worm gear 5-10 drives worm wheel shaft 5-11 around own axes reciprocating rotation, and worm wheel shaft 5-11 drives two drive bevel gears
For 5-12 around own axes reciprocating rotation, two drive bevel gear 5-12 drive two driving pulley assemblies 6 to work respectively.
Specific embodiment 6:
Illustrate present embodiment below with reference to Fig. 1-11, the driving pulley assembly 6 includes driving gear 6-1, gear turn
Axis 6-2, U-shaped pinion stand 6-3, driven wheel of differential 6-4, vertical rack gear 6-5 and shift fork 6-6；Driving gear 6-1 is fixedly connected on tooth
It takes turns on shaft 6-2, rotary gear shaft 6-2 is rotatably connected on pinion stand 6-3 by rolling bearing units, and pinion stand 6-3 is fixedly connected on
Upper end on the inside of bowl body 2-1, driven wheel of differential 6-4 are fixedly connected on rotary gear shaft 6-2, driven wheel of differential 6-4 with
Drive bevel gear 5-12 is connected by meshing transmission；The driving gear 6-1 is connected by meshing transmission with vertical rack gear 6-5, vertical rack gear
6-5 is located in U-shaped pinion stand 6-3, and the outer end face of vertical rack gear 6-5 is bonded with the medial surface of U-shaped pinion stand 6-3, vertical rack gear
The lower end of 6-5 is fixedly connected with shift fork 6-6, and shift fork 6-6 clearance fit is connected on mixing component 7.The driving pulley assembly 6 exists
In use, drive bevel gear 5-12 drives driven wheel of differential 6-4 around own axes reciprocating rotation, driven wheel of differential 6-4 band movable tooth
Shaft 6-2 is taken turns around own axes reciprocating rotation, rotary gear shaft 6-2 drives driving gear 6-1 around own axes reciprocating rotation, driving
Gear 6-1 drives vertical rack gear 6-5 to move up and down, and vertical rack gear 6-5 drives shift fork 6-6 to move up and down；Vertical rack gear 6-5's is outer
End face is bonded with the medial surface of U-shaped pinion stand 6-3, and U-shaped pinion stand 6-3 plays the role of limit to vertical rack gear 6-5, and it is vertical to make
Rack gear 6-5 can only be moved up and down.
Specific embodiment 7:
Illustrate present embodiment below with reference to Fig. 1-11, the mixing component 7 includes central axis 7-1, driven pulley 7-
2, partial gear 7-3, disk 7-4, axle sleeve 7-5, annular groove 7-6, lateral shelf plate 7-7, blade rotor 7-8 and stirring blade 7-9；
The both ends of central axis 7-1 pass through rolling bearing units respectively and are rotatably connected on horizontal frame plate 4-1 and bowl body 2-1 inside bottom surface
Center, upper end, middle-end and the lower end of central axis 7-1 are respectively fixedly connected with driven pulley 7-2, partial gear 7-3 and disk 7-
4, driven pulley 7-2 connect driving pulley 4-4,8 engaged transmission of partial gear 7-3 and anti-blocking plug assembly by belt V belt translation
Connection, disk 7-4 are slidably connected in axle sleeve 7-5, and it is sliding that a perforative convex block is respectively set in the rear and front end of axle sleeve 7-5
Slot is fixedly connected with two convex blocks on disk 7-4, and two convex blocks are slidably connected respectively in two convex block sliding slots, axle sleeve 7-5
Left and right ends be respectively fixedly connected on lateral shelf plate a 7-7, two lateral shelf plate 7-7 and be respectively fixedly connected with a blade rotor 7-
A stirring blade 7-9 is respectively fixedly connected on 8, two blade rotor 7-8；The upper end of axle sleeve 7-5 is provided with annular groove 7-6,
Clearance fit is connected to the both ends of annular groove 7-6 respectively for the inside of two shift fork 6-6.The mixing component 7 is when in use, main
Movable belt pulley 4-4 drives driven pulley 7-2 around own axes reciprocating rotation by belt, driven pulley 7-2 drive central axis 7-1 around
Own axes reciprocating rotation, central axis 7-1 drive partial gear 7-3 and disk 7-4 around own axes reciprocating rotation, not exclusively
Gear 7-3 drives anti-blocking plug assembly 8 to work, and disk 7-4 drives axle sleeve 7-5 around own axes reciprocating rotation by two convex blocks,
Axle sleeve 7-5 drives the stirring blade 7-9 on two blade rotor 7-8 to move in a circle by two lateral shelf plate 7-7, realizes rotation
Stir work；Axle sleeve 7-5 is driven to move up and down when two shift fork 6-6 are moved up and down, the convex block on disk 7-4 can be in convex block sliding slot
Interior sliding, axle sleeve 7-5 drive two stirring blade 7-9 to carry out stirring work up and down, realize two stirring blade 7-9 or more with
Rotate the work being mixed.
Specific embodiment 8:
Illustrate present embodiment below with reference to Fig. 1-11, the anti-blocking plug assembly 8 includes agitating shaft 8-1, axle bed plate 8-
2, linkage gear 8-3 and agitating plate 8-4；The upper and lower ends of agitating shaft 8-1 pass through rolling bearing units respectively and are rotatably connected on axle bed plate
On bottom surface on the inside of 8-2 and bowl body 2-1, axle bed plate 8-2 is fixedly connected on bowl body 2-1 medial surface, linkage
Gear 8-3 is fixedly connected on the upper end of agitating shaft 8-1, and linkage gear 8-3 is connected by meshing transmission with partial gear 7-3, stirring
The lower end of axis 8-1 is fixedly connected with muti-piece agitating plate 8-4；The agitating plate 8-4 is located at the rear end of sieve 2-2.The anti-clogging
When in use, partial gear 7-3 reciprocating rotation drives linkage gear 8-3 around own axis, linkage gear 8-3 to component 8
Drive agitating shaft 8-1 around own axis, agitating shaft 8-1 drives muti-piece agitating plate 8-4 rotation, and agitating plate 8-4 is to sieve 2-2
Neighbouring mixed liquor is stirred, and avoids the problem that the filter opening I on sieve 2-2 is blocked and influences cell separative efficiency by cell.
Specific embodiment 9:
Illustrate present embodiment below with reference to Fig. 1-11, the aperture of the filter opening I, filter opening II and filter opening III is successively passed
Subtract；The material of sieve 2-2, the first sieve plate 3-3 and the second sieve plate 3-4 are all stainless steel.The aperture of filter opening III is less than to be separated thin
The minimum diameter of born of the same parents.
A kind of method of cell separation, method includes the following steps:
Step 1: Power Component 4 is powered after unlatching, and Power Component 4 drives two stirring blade 7-9 on mixing component 7
Carry out Stirring and upper and lower stirring movement；
Step 2: mixed liquor is persistently poured into bowl body 2-1, and two stirring blade 7-9 stir mixed solution
Separation, the cell and waste liquid isolated are flowed out by the filter opening I on sieve 2-2, realize side feed liquor, side separation, side drain；
Step 3: the cell isolated first is fallen on the first sieve plate 3-3, and the cell that cell size is greater than filter opening II, which slides, to be received
Collecting into the first collection box 3-7, the cell that cell size is less than filter opening II passes through filter opening II and falls on the second sieve plate 3-4, and second
Cell on sieve plate 3-4 is collected into the second collection box 3-8, is realized and is carried out steps filtering, sieve to various sizes of cell
Divide and separates.
The working principle of a kind of cell separation system of the present invention and cell isolation method:
In the use of the present invention, Power Component 4 drives mixing component 7 to be revolved after Power Component 4 is connected to power initiation
Turn stirring work, while Power Component 4 also drives reciprocating drive mechanism 5 to work, reciprocating drive mechanism 5 drives two driving wheel groups
Part 6 works, and mixed liquor to be separated is held in the stirring work up and down that two driving pulley assemblies 6 drive mixing component 7 to be recycled
Continuous to pour into separation cartridge module 2, mixing component 7 be mixed with rotation up and down to mixed liquor, is sufficiently separated cell, point
The cell and mixed liquor separated out is discharged from the filter opening I of separation 2 lower end of cartridge module, the filter opening II and filter opening III in Screen assemblies 3
Aperture successively successively decrease, various sizes of cell is filtered, sieve, is separated, preferable is kept to various sizes of cell
Rejection, mixing component 7 also drive anti-blocking plug assembly 8 to work, and anti-blocking plug assembly 8 avoids filter opening I from blocking and influences cell separation
The problem of efficiency, on one side feed liquor, on one side separate, while drain improve cell separation efficiency.
Certainly, above description is not limitation of the present invention, and the present invention is also not limited to the example above, the art
The variations, modifications, additions or substitutions that those of ordinary skill is made within the essential scope of the present invention also belong to guarantor of the invention
Protect range.
Claims (10)
1. a kind of cell separation system, including it is pedestal (1), separation cartridge module (2), Screen assemblies (3), Power Component (4), reciprocal
Driving mechanism (5), driving pulley assembly (6), mixing component (7) and anti-blocking plug assembly (8), it is characterised in that: the cylinder
Component (2) is fixedly connected on pedestal (1), and Screen assemblies (3) are fixedly connected between pedestal (1) and separation cartridge module (2), is moved
Power component (4) is fixedly connected on the upper end of separation cartridge module (2), and reciprocating drive mechanism (5) is fixedly connected on separation cartridge module (2)
Internal upper end, Power Component (4) are slidably connected on reciprocating drive mechanism (5), and driving pulley assembly (6) is provided with two
A, two driving pulley assemblies (6) are symmetrically fixedly connected in separation cartridge module (2), the both ends of reciprocating drive mechanism (5) respectively with
Two driving pulley assemblies (6) are connected by meshing transmission, and mixing component (7) is connected on Power Component (4) and separation cartridge module (2),
The inner end of two driving pulley assemblies (6) is all connect with mixing component (7) clearance fit, and anti-blocking plug assembly (8) is fixedly connected on point
Front end from cartridge module (2), anti-blocking plug assembly (8) are connected by meshing transmission with mixing component (7).
2. a kind of cell separation system according to claim 1, it is characterised in that: the separation cartridge module (2) includes
Bowl body (2-1), sieve (2-2), arc seat board (2-3) and supporting leg (2-4)；The front end of bowl body (2-1) is set
It is equipped with discharge through-hole, is fixedly connected on the inside of discharge through-hole sieve (2-2), filter opening I, cylinder sheet are provided on sieve (2-2)
The lower end of body (2-1) is symmetrically fixedly connected with two arc seat boards (2-3), and the lower end of two arc seat boards (2-3) is fixed respectively to be connected
Two supporting legs (2-4) are connect, four supporting legs (2-4) are all fixedly connected on pedestal (1).
3. a kind of cell separation system according to claim 2, it is characterised in that: the Screen assemblies (3) include perpendicular
Plate (3-1), transverse slat (3-2), the first sieve plate (3-3), the second sieve plate (3-4), side plate (3-5), L-type seat board (3-6), first are collected
Box (3-7) and the second collection box (3-8)；The both ends of riser (3-1) are respectively fixedly connected in bowl body (2-1) and pedestal
(1), the front end of riser (3-1) is fixedly connected with two transverse slats (3-2), and the first sieve plate (3-3) and the second sieve plate (3-4) are fixed respectively
It is connected on two transverse slats (3-2), the second sieve plate (3-4) is located at the lower section of the first sieve plate (3-3), and the first sieve plate (3-3) is to the left
Inclined downward, the second sieve plate (3-4) tilt to the right, and the left and right ends of transverse slat (3-2) are respectively fixedly connected with a side plate (3-
5) it, is respectively fixedly connected with a L-type seat board (3-6) on two side plates (3-5), is respectively set first on two L-type seat boards (3-6)
Collection box (3-7) and the second collection box (3-8), the first collection box (3-7) are located at the lower section of the left end the first sieve plate (3-3), and second
Collection box (3-8) is located at the lower section of the second sieve plate (3-4) right end；Filter opening II, the second sieve are provided on first sieve plate (3-3)
Filter opening III is provided on plate (3-4)；First sieve plate (3-3) is located at the lower section of sieve (2-2).
4. a kind of cell separation system according to claim 3, it is characterised in that: the Power Component (4) includes water
Flatrack plate (4-1), motor (4-2), transmission shaft (4-3), driving pulley (4-4), bull stick (4-5), driving lever (4-6) and L-type pedestal
Plate (4-7)；Horizontal frame plate (4-1) is fixedly connected on the upper end of bowl body (2-1), and motor (4-2) is fixed by motor rack
It is connected on horizontal frame plate (4-1), the output shaft of motor (4-2) connects transmission shaft (4-3) by shaft coupling, transmission shaft (4-3)
Middle-end be rotatably connected on horizontal frame plate (4-1) by rolling bearing units, driving pulley (4-4) and bull stick (4-5) are fixed respectively
The upper and lower ends of transmission shaft (4-3) are connected to, driving pulley (4-4) and mixing component (7) are sequentially connected by belt, bull stick
The outer end of (4-5) is fixedly connected driving lever (4-6), and two L-type pedestal plates (4-7) are symmetrically fixedly connected on horizontal frame plate (4-1)
Left and right ends.
5. a kind of cell separation system according to claim 4, it is characterised in that: reciprocating drive mechanism (5) packet
Include rectangle sliding sash (5-1), T-type sliding block (5-2), trapezoidal raised line (5-3), fixed transverse slat (5-4), connecting plate (5-5), horizontal rack
(5-6), driving gear (5-7), worm screw (5-8), bridge plate (5-9), worm gear (5-10), worm wheel shaft (5-11) and active conical tooth
It takes turns (5-12)；The rear and front end of rectangle sliding sash (5-1) is respectively fixedly connected with connecting plate (5-5) and T-type sliding block (5-2), T-type sliding block
(5-2) is slidably connected on trapezoidal raised line (5-3), and trapezoidal raised line (5-3) is fixedly connected on fixed transverse slat (5-4), Gu
Determine the upper end that transverse slat (5-4) is fixedly connected on the inside of bowl body (2-1)；The lower end of the connecting plate (5-5) is fixedly connected
Horizontal rack (5-6), horizontal rack (5-6) are connected by meshing transmission driving gear (5-7), and driving gear (5-7) is fixedly connected on
The rear end of the middle-end of worm screw (5-8), worm screw (5-8) is rotatably connected on bridge plate (5-9) by rolling bearing units, bridge plate (5-
9) it is fixedly connected on fixed transverse slat (5-4), the front end of worm screw (5-8) is connected by meshing transmission worm gear (5-10), worm gear (5-10)
It is fixedly connected on the middle-end of worm wheel shaft (5-11), the both ends of worm wheel shaft (5-11) are rotatably connected on by rolling bearing units respectively
On two L-type pedestal plates (4-7), the outer end at the both ends worm wheel shaft (5-11) is respectively fixedly connected with a drive bevel gear (5-
12)；The drive bevel gear (5-12) is connected by meshing transmission with driving pulley assembly (6)；Driving lever (4-6) clearance fit is connected to square
In shape sliding sash (5-1).
6. a kind of cell separation system according to claim 5, it is characterised in that: the driving pulley assembly (6) includes
It drives gear (6-1), rotary gear shaft (6-2), U-shaped pinion stand (6-3), driven wheel of differential (6-4), vertical rack gear (6-5) and dials
It pitches (6-6)；Driving gear (6-1) is fixedly connected on rotary gear shaft (6-2), and rotary gear shaft (6-2) is rotated by rolling bearing units
It is connected on pinion stand (6-3), pinion stand (6-3) is fixedly connected on the upper end on the inside of bowl body (2-1), driven wheel of differential
(6-4) is fixedly connected on rotary gear shaft (6-2), and driven wheel of differential (6-4) is connected by meshing transmission with drive bevel gear (5-12)；
The driving gear (6-1) is connected by meshing transmission with vertical rack gear (6-5), and vertical rack gear (6-5) is located at U-shaped pinion stand (6-3)
Interior, the outer end face of vertical rack gear (6-5) is bonded with the medial surface of U-shaped pinion stand (6-3), and the lower end of vertical rack gear (6-5) is fixed
It connects shift fork (6-6), shift fork (6-6) clearance fit is connected on mixing component (7).
7. a kind of cell separation system according to claim 6, it is characterised in that: during the mixing component (7) includes
Mandrel (7-1), driven pulley (7-2), partial gear (7-3), disk (7-4), axle sleeve (7-5), annular groove (7-6), side frame
Plate (7-7), blade rotor (7-8) and stirring blade (7-9)；The both ends of central axis (7-1) are rotated by rolling bearing units respectively to be connected
It connects in the center of horizontal frame plate (4-1) and bowl body (2-1) inside bottom surface, upper end, middle-end and the lower end of central axis (7-1)
It is respectively fixedly connected with driven pulley (7-2), partial gear (7-3) and disk (7-4), driven pulley (7-2) passes through belt band
It is sequentially connected driving pulley (4-4), partial gear (7-3) is connected by meshing transmission with anti-blocking plug assembly (8), and disk (7-4) is sliding
Dynamic to be cooperatively connected in axle sleeve (7-5), a perforative convex block sliding slot, disk (7- is respectively set in the rear and front end of axle sleeve (7-5)
4) two convex blocks are fixedly connected on, two convex blocks are slidably connected respectively in two convex block sliding slots, the left and right of axle sleeve (7-5)
Both ends are respectively fixedly connected with a lateral shelf plate (7-7), are respectively fixedly connected with a blade rotor (7- on two lateral shelf plates (7-7)
8), a stirring blade (7-9) is respectively fixedly connected on two blade rotors (7-8)；The upper end of axle sleeve (7-5) is provided with annular
Slot (7-6), clearance fit is connected to the both ends of annular groove (7-6) respectively for the inside of two shift forks (6-6).
8. a kind of cell separation system according to claim 7, it is characterised in that: the anti-blocking plug assembly (8) includes
Agitating shaft (8-1), axle bed plate (8-2), linkage gear (8-3) and agitating plate (8-4)；The upper and lower ends of agitating shaft (8-1) are distinguished
It is rotatably connected on the bottom surface on the inside of axle bed plate (8-2) and bowl body (2-1) by rolling bearing units, axle bed plate (8-2) is solid
Surely it is connected on bowl body (2-1) medial surface, linkage gear (8-3) is fixedly connected on the upper end of agitating shaft (8-1), linkage
Gear (8-3) is connected by meshing transmission with partial gear (7-3), and the lower end of agitating shaft (8-1) is fixedly connected with muti-piece agitating plate (8-
4)；The agitating plate (8-4) is located at the rear end of sieve (2-2).
9. a kind of cell separation system according to claim 8, it is characterised in that: filter opening I, filter opening II and the filter opening
Successively successively decrease in III aperture；The material of sieve (2-2), the first sieve plate (3-3) and the second sieve plate (3-4) is all stainless steel.
10. the method for carrying out cell separation using a kind of cell separation system as claimed in claim 9, it is characterised in that: the party
Method the following steps are included:
Step 1: Power Component (4) is powered after unlatching, and Power Component (4) drives two stirring blades on mixing component (7)
(7-9) carries out Stirring and upper and lower stirring movement；
Step 2: mixed liquor persistently being poured into bowl body (2-1), and two stirring blades (7-9) stir mixed solution
Separation, the cell and waste liquid isolated are flowed out by the filter opening I on sieve (2-2), realize side feed liquor, side separation, side drain；
Step 3: the cell isolated first is fallen on the first sieve plate (3-3), and the cell that cell size is greater than filter opening II, which slides, to be collected
In into the first collection box (3-7), the cell that cell size is less than filter opening II passes through filter opening II and falls on the second sieve plate (3-4), the
Cell on two sieve plates (3-4) is collected into the second collection box (3-8), and realization carries out steps mistake to various sizes of cell
Filter, screening and separation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910265880.8A CN109913363B (en) | 2019-04-03 | 2019-04-03 | Cell separation system and cell separation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910265880.8A CN109913363B (en) | 2019-04-03 | 2019-04-03 | Cell separation system and cell separation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109913363A true CN109913363A (en) | 2019-06-21 |
| CN109913363B CN109913363B (en) | 2020-04-10 |
Family
ID=66968360
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910265880.8A Active CN109913363B (en) | 2019-04-03 | 2019-04-03 | Cell separation system and cell separation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109913363B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110420592A (en) * | 2019-07-31 | 2019-11-08 | 倪亚丽 | A kind of chemical industry solutions automatically configure equipment |
| CN111073796A (en) * | 2020-03-04 | 2020-04-28 | 山东第一医科大学(山东省医学科学院) | Special separator of circulation tumor cell |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017083705A1 (en) * | 2015-11-13 | 2017-05-18 | The Johns Hopkins University | Cell culture system and method of use thereof |
| CN108060082A (en) * | 2017-12-29 | 2018-05-22 | 天津普瑞赛尔生物科技有限公司 | A kind of stem cell filter separator |
| CN108855928A (en) * | 2018-08-29 | 2018-11-23 | 武汉新国峰科技开发有限公司 | A kind of bio-pharmaceuticals screening installation |
| CN109453542A (en) * | 2018-12-27 | 2019-03-12 | 张隆荣 | A kind of ultrasonic extraction tank equipped with filter device |
-
2019
- 2019-04-03 CN CN201910265880.8A patent/CN109913363B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017083705A1 (en) * | 2015-11-13 | 2017-05-18 | The Johns Hopkins University | Cell culture system and method of use thereof |
| CN108060082A (en) * | 2017-12-29 | 2018-05-22 | 天津普瑞赛尔生物科技有限公司 | A kind of stem cell filter separator |
| CN108855928A (en) * | 2018-08-29 | 2018-11-23 | 武汉新国峰科技开发有限公司 | A kind of bio-pharmaceuticals screening installation |
| CN109453542A (en) * | 2018-12-27 | 2019-03-12 | 张隆荣 | A kind of ultrasonic extraction tank equipped with filter device |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110420592A (en) * | 2019-07-31 | 2019-11-08 | 倪亚丽 | A kind of chemical industry solutions automatically configure equipment |
| CN111073796A (en) * | 2020-03-04 | 2020-04-28 | 山东第一医科大学(山东省医学科学院) | Special separator of circulation tumor cell |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109913363B (en) | 2020-04-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN211330237U (en) | Sieving mechanism is used in rice processing | |
| CN109913363A (en) | A kind of cell separation system and cell isolation method | |
| CN111013767A (en) | A grinder for chinese-medicinal material processing | |
| CN211756860U (en) | Structure for reducing dust content in sand through filter stone for sand washer | |
| CN212790104U (en) | Sweet potato starch production is with deposiing device | |
| CN116921078B (en) | Abidol hydrochloride centrifugal machine for production | |
| CN213349671U (en) | Green tea trembles sieve machine convenient to maintenance | |
| CN211912876U (en) | Fungus residue multi-stage filtering device for preparing edible fungus composite polysaccharide | |
| CN113058313A (en) | Filtering process for producing and processing skimmed milk powder | |
| CN210357531U (en) | Brewing raw material water floats sieving mechanism | |
| CN218392665U (en) | A sediment liquid separator for fruit wine fermentation | |
| CN111318450B (en) | Automatic change diamond micropowder layering sorting unit | |
| CN109550294A (en) | A kind of self-cleaning filter | |
| CN115228730A (en) | Vibrating type impurity removal equipment for lithium battery raw materials and impurity removal method thereof | |
| CN113528260A (en) | Food fermentation system and method | |
| CN112661152A (en) | Pretreatment method for raw material for preparing powdered activated carbon | |
| CN217888630U (en) | A high-efficient tea foam separator for tealeaves production | |
| CN219002253U (en) | Coal slime solid-liquid separation sieve basket | |
| CN218130490U (en) | Automatic filter for boiling lees | |
| CN217809398U (en) | Liver-derived stem cell separation device | |
| CN216825112U (en) | Filtration and separation device for camellia oil purification | |
| CN220968833U (en) | Processing deslagging machine for white granulated sugar | |
| CN211734029U (en) | Glass processing sewage treatment system | |
| CN219785154U (en) | Tea beverage production centrifugal device | |
| CN221740214U (en) | Juice filtering device for beer production |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20200317 Address after: 102200 Changping District science and Technology Park, Zhongguancun Life Science Park, life Park Road, No. 8, No. 1 hospital, building 1, floor 10, floor Applicant after: BEIJING TAISHENG BIOLOGICAL TECHNOLOGY Co.,Ltd. Address before: 161000 1 groups of Haig village, Changqing Township, Fulaerji District, Qigihar, Heilongjiang Applicant before: He Chunlan |
|
| TA01 | Transfer of patent application right | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |