CN111257080A - Assay sample processing apparatus for hepatobiliary surgery - Google Patents
Assay sample processing apparatus for hepatobiliary surgery Download PDFInfo
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- CN111257080A CN111257080A CN202010132067.6A CN202010132067A CN111257080A CN 111257080 A CN111257080 A CN 111257080A CN 202010132067 A CN202010132067 A CN 202010132067A CN 111257080 A CN111257080 A CN 111257080A
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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Abstract
The invention discloses an assay sample processing device for hepatobiliary surgery, which is characterized by comprising: the device comprises a base, a control box, a conveyor belt rotating shaft, a power line, a PLC (programmable logic controller), a conveyor belt control knob, a control button, a glass slide, an inoculation telescopic rod, an inoculation transverse rod, a cell sap dropper, a scraping telescopic rod, a scraping transverse telescopic rod, a scraping cotton head connecting rod, a scraping cotton head, a cover glass telescopic rod, a cover glass transverse rod, a vacuum sucker telescopic rod and a vacuum sucker; the device design machinery is automatic, and cell inoculation, cell scrape coating, cover glass slide and make and cultivate the sample and accomplish in proper order, and the manual work only needs to carry out control operation, and whole process realizes streamlined operation, especially is fit for the preparation of large batch cell culture sample.
Description
Technical Field
The invention belongs to the field of medical specimen assay, and relates to an assay specimen processing device for hepatobiliary surgery.
Background
Liver and gall surgery mainly studies hepatocellular carcinoma, hepatobiliary calculus, posthepatitic cirrhosis and acute liver failure caused by severe hepatitis are serious diseases threatening human health. Puncture cells are obtained by puncturing the lesion part, and canceration can be determined or eliminated conveniently after the cells are cultured. Cell culture requires scraping of punctured cells onto a glass slide to make a culture specimen, which is observed and detected after a period of culture. Present assay sample processing apparatus single structure, degree of mechanization is low, needs the manual work to carry out the knife coating to the cell, and when cultivateing the great time of cell quantity, manpower work load is big, and easily causes the knife coating inhomogeneous, influences the cell observation and the detection after cultivateing.
Disclosure of Invention
In order to solve the technical problems, the invention designs the test specimen processing device for hepatobiliary surgery, which is designed to be mechanically automated, and sequentially complete cell inoculation, cell scraping and cover glass slide preparation and culture specimen preparation, only needs to be manually controlled, realizes streamlined operation in the whole process, and is particularly suitable for large-batch cell culture specimen preparation.
In order to realize the technical effects, the invention is realized by the following technical scheme: an assay sample processing device for hepatobiliary surgery, comprising: the device comprises a base, a control box, a conveyor belt rotating shaft, a power line, a PLC (programmable logic controller), a conveyor belt control knob, a control button, a glass slide, an inoculation telescopic rod, an inoculation transverse rod, a cell sap dropper, a scraping telescopic rod, a scraping transverse telescopic rod, a scraping cotton head connecting rod, a scraping cotton head, a cover glass telescopic rod, a cover glass transverse rod, a vacuum sucker telescopic rod and a vacuum sucker;
the base structure is a cuboid, the upper end face of the base structure is open, and the base structure is hollow; the right end face of the base is connected with a control box; the left side and the right side in the base are respectively provided with a conveying belt rotating shaft, and the conveying belt rotating shafts are connected with conveying belts; a power supply is arranged in the control box and connected to each electromechanical component, and a power line is led out from the inside and connected to the right side surface of the control box; a PLC controller is arranged in the control box; the upper surface of the control box is connected with a conveyor belt control knob; the front of the control box is provided with control buttons of all parts; placing a glass slide on the conveying belt; the left side behind the base is fixedly connected with an inoculation telescopic rod, the head end of the inoculation telescopic rod is fixedly connected with an inoculation cross rod, the head end of the inoculation cross rod is provided with a through hole, and a cell sap dropper is detachably connected in the through hole; the middle part of the back of the base is fixedly connected with a scraping telescopic rod, the head end of the scraping telescopic rod is fixedly connected with a scraping transverse rod, the interior of the scraping transverse rod is telescopically connected with a scraping transverse telescopic rod, the head end of the scraping transverse telescopic rod is fixedly connected with a scraping cotton head connecting rod, and the lower end of the scraping cotton head connecting rod is detachably connected with a scraping cotton head; the right side of the back of the base is fixedly connected with a cover glass telescopic rod, the head end of the cover glass telescopic rod is fixedly connected with a cover glass cross rod, the head end of the cover glass cross rod is fixedly connected with two vacuum sucker telescopic rods, and the lower ends of the vacuum sucker telescopic rods are connected with vacuum suckers;
furthermore, the PLC is connected with a conveyor belt rotating shaft, the conveyor belt rotating shaft is connected with a conveyor belt control knob through a power cord, the PLC is preset with a program, the conveyor belt rotating shaft is controlled to rotate to a specific position and then automatically stops, and the conveyor belt control knob needs to be manually controlled to rotate;
furthermore, three rows of control buttons are vertically arranged and respectively correspond to three functions of controlling inoculation, blade coating and cover glass;
furthermore, the vacuum chuck is used for sucking the glass slide when the vacuum chuck is powered on and dropping the glass slide when the vacuum chuck is powered off;
further, the scraping and coating cotton head is in a strip shape;
the invention also aims to provide a quantifiable cell sap dropper which consists of a push rod grab handle, a refrigeration outer cylinder, a push rod, a piston, a spring limiting part, a spring pressing block, a liquid adding opening, a quantitative liquid storage part switch and a dropper head; the refrigerating outer cylinder has a refrigerating function, the lower end of the refrigerating outer cylinder is connected with a quantitative liquid storage part, the quantitative liquid storage part is controlled by a switch, and the lower end of the quantitative liquid storage part is provided with a liquid dropper; the cold storage outer cylinder is fixedly connected with a spring limiting part close to the upper end part, a through hole is formed in the middle of the spring limiting part, and a spring is fixedly connected with the spring limiting part; a liquid feeding port is connected to the refrigerating outer cylinder; the push rod penetrates through the upper end of the refrigeration outer barrel and the spring limiting part and reaches the lower end of the refrigeration outer barrel, the lower end of the push rod is connected with the piston, and the outer diameter of the piston is equal to the inner diameter of the refrigeration outer barrel; the upper part of the push rod is fixedly connected with a spring pressing block;
the quantitative cell sap dropper has the working principle that: adding cell liquid into the refrigeration outer cylinder from the liquid adding port, pressing the push rod grab handle, and driving the push rod, the spring pressing block and the piston to move downwards together; the spring is extruded and deformed, the piston is pushed into the quantitative liquid storage part, the cell liquid starts to flow into the quantitative liquid storage part, after the quantitative liquid storage part is filled with the cell liquid, the grab handle of the push rod is loosened, the spring is restored, the spring is driven to press the pressing block, the push rod is pushed, the piston is upward, and the piston plugs the lower end of the refrigeration outer cylinder; the quantitative liquid storage part stores quantitative cell liquid; controlling the switch of the quantitative liquid storage part to make the cell liquid flow out from the liquid dropping head.
Another object of the present invention is to provide a method of using an assay sample processing device for hepatobiliary surgery, the method comprising:
firstly, connecting a device power line to a power supply;
cleaning the conveying belt to clean the surface, and placing the glass slide on the conveying belt and right below the cell sap dropper, or padding a clean isolation pad under the glass slide;
thirdly, controlling a button of an inoculation telescopic rod to drive a cell sap dropper to descend to a proper height above the glass slide;
pressing a handle part of a push rod of the cell sap dropper to enable cell sap in the refrigeration outer cylinder to enter a liquid storage part filled with quantitative liquid, loosening the handle part of the push rod, storing the quantitative cell sap in the liquid storage part, and controlling a switch of the liquid storage part to enable the cell sap to be dripped in the middle of the glass slide;
fifthly, operating a control knob of the conveyor belt, moving the glass slide to the position right below the scraping cotton head on the conveyor belt, and automatically stopping the conveyor belt;
sixthly, controlling a scraping and coating telescopic rod control button to drive a scraping and coating cotton head to descend to a proper height above the glass slide, and controlling a scraping and coating transverse telescopic rod button to stretch back and forth to uniformly scrape and coat the cell sap;
seventhly, operating the control knob of the conveyor belt to enable the conveyor belt to continuously drive the glass slide to rotate to the position right below the cover glass, and automatically stopping the conveyor belt;
eighthly, controlling a control button of a telescopic rod of the cover glass to enable the cover glass to be driven to descend to a proper height above the glass slide, controlling a telescopic rod button of a vacuum sucker to descend to enable one telescopic rod of the vacuum sucker to enable one side of the cover glass to be attached to one side of the glass slide, and then enabling one telescopic rod of the vacuum sucker to descend to enable the cover glass to slowly cover the upper side of the glass slide;
ninthly, disconnecting the power of the vacuum sucker to ensure that the sucker loosens the cover glass;
the invention has the beneficial effects that: the laboratory sample processing device for hepatobiliary surgery is mechanized in structure, and can perform inoculation, scraping and cover glass streamlined operation, wherein each part of the laboratory sample processing device only needs to be manually operated by a button without manual inoculation, scraping and cover glass; the quantitative cell sap dropper can ensure that the cell sap dripped on the glass slide is uniform every time, so that the phenomenon of nonuniform volume caused by manual inoculation is avoided, and the cell sap dropper adopts a refrigeration outer barrel, so that the cell sap in the cell sap dropper can be in a low-level environment, and the cell activity is kept; the automatic scraping function is matched with the strip-shaped scraping cotton head, so that cells can be uniformly scraped, and the uniformity of the cells cannot be ensured by manual scraping; the cover glass needs to be careful when in cover glass production, when a large number of cell culture samples are produced, the manual work load is increased by the manual cover glass, the manual work is saved by the automatic cover glass, and the cover glass can be accurately placed above the glass slide; the conveying belt controlled by the PLC can enable the glass slide driven by the conveying belt to stop at the same position every time, and therefore blade coating and accurate operation of cover glass are facilitated.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic view showing the construction of an assay sample processing device for hepatobiliary surgery;
FIG. 2 is a schematic view showing the internal structure of an assay sample processing device for hepatobiliary surgery;
FIG. 3 is a schematic view of a cell drip tube structure 1 of an assay sample processing device for hepatobiliary surgery;
FIG. 4 is a schematic diagram of a pipette structure of the specimen processing device for hepatobiliary surgery, FIG. 2;
FIG. 5 is a schematic structural view of the specimen processing device according to example 4 for hepatobiliary surgery;
in the drawings, the components represented by the respective reference numerals are listed below:
1-base, 2-control box, 3-conveyor belt, 4-conveyor belt rotating shaft, 5-power line, 6-PLC controller, 7-conveyor belt control knob, 8-control button, 9-glass slide, 10-inoculation telescopic rod, 11-inoculation transverse rod, 12-cell sap dropper, 121-push rod grab handle, 122-refrigeration outer cylinder, 123-push rod, 124-piston, 125-spring limiting part, 126-spring, 127-spring pressing block, 128-liquid adding opening, 129-quantitative liquid storage part, 1210-quantitative liquid storage part switch, 1211-liquid dropping head, 13-knife coating telescopic rod, 14-knife coating transverse rod, 15-knife coating transverse telescopic rod, 16-knife coating cotton head connecting rod, 17-knife coating cotton head, 18-cover glass telescopic rod, 19-cover glass cross bar, 20-vacuum sucker telescopic bar, 21-vacuum sucker, 22-limit switch.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
Referring to fig. 1 to 4, an assay sample processing device for hepatobiliary surgery includes: the cell culture device comprises a base 1, a control box 2, a conveyor belt 3, a conveyor belt rotating shaft 4, a power line 5, a PLC (programmable logic controller) 6, a conveyor belt control knob 7, a control button 8, a glass slide 9, an inoculation telescopic rod 10, an inoculation transverse rod 11, a cell sap dropper 12, a scraping telescopic rod 13, a scraping transverse rod 14, a scraping transverse telescopic rod 15, a scraping cotton head connecting rod 16, a scraping cotton head 17, a cover glass telescopic rod 18, a cover glass transverse rod 19, a vacuum sucker telescopic rod 20 and a vacuum sucker 21;
the base 1 is in a cuboid structure, the upper end face of the base is open, and the base is hollow; the right end face of the base 1 is connected with a control box 2; a conveyor belt rotating shaft 4 is respectively arranged at the left side and the right side in the base 1, and the conveyor belt 3 is connected to the conveyor belt rotating shaft 4; a power supply is arranged in the control box 2 and connected to each electromechanical component, and a power line 5 is led out from the inside and connected to the right side surface of the control box 2; a PLC (programmable logic controller) 6 is arranged in the control box 2; the upper surface of the control box 2 is connected with a conveyor belt control knob 7; the front of the control box 2 is provided with a control button 8 of each component; a slide glass 9 is placed on the conveyor belt 3; the left side of the back of the base 1 is fixedly connected with an inoculation telescopic rod 10, the head end of the inoculation telescopic rod 10 is fixedly connected with an inoculation cross rod 11, the head end of the inoculation cross rod 11 is provided with a through hole, and a cell sap dropper 12 is detachably connected in the through hole; a scraping telescopic rod 13 is fixedly connected to the middle of the back of the base 1, a scraping transverse rod 14 is fixedly connected to the head end of the scraping telescopic rod 13, a scraping transverse telescopic rod 15 is telescopically connected to the inside of the scraping transverse rod 14, a scraping cotton head connecting rod 16 is fixedly connected to the head end of the scraping transverse telescopic rod 15, and a scraping cotton head 17 is detachably connected to the lower end of the scraping cotton head connecting rod 16; the right side of the back of the base 1 is fixedly connected with a cover glass telescopic rod 18, the head end of the cover glass telescopic rod 18 is fixedly connected with a cover glass cross rod 19, the head end of the cover glass cross rod 19 is fixedly connected with two vacuum sucker telescopic rods 20, and the lower ends of the vacuum sucker telescopic rods 20 are connected with vacuum suckers 21;
the PLC 6 is connected with the conveyor belt rotating shaft 4, the conveyor belt rotating shaft 4 is connected with the conveyor belt control knob 7 through the power cord 5, the PLC 6 is preset with a program, the conveyor belt rotating shaft 4 is controlled to rotate to a specific position and automatically stops, and the conveyor belt control knob 7 needs to be manually controlled to rotate; the position where the slide glass 9 stops on the conveyor belt 3 is just opposite to the position where the cotton head 17 and the cover glass are scraped;
three rows of control buttons 8 are vertically arranged and respectively correspond to three functions of inoculation, blade coating and cover glass control;
the vacuum sucker 21 sucks the glass slide when the power is on, and the glass slide falls when the power is off;
the scraping cotton head 17 is in a strip shape; the scrape coating cotton head 17 can be replaced, and the scrape coating is even guaranteed by the long-strip-shaped cotton head.
Example 2
A kind of hepatobiliary surgery uses the specimen processing unit of the assay, its quantitative cell sap dropper 12, it is made up of push rod grab handle 121, cold storage outer cylinder 122, push rod 123, piston 124, spring spacing part 125, spring 126, spring pressing block 127, filling opening 128, quantitative stock solution part 129, quantitative stock solution part switch 1210, drip 1211; the refrigerating outer cylinder 122 has refrigerating function, the lower end is connected with a quantitative liquid storage part 129, a quantitative liquid storage part switch 1210 is used for controlling, and the lower end of the quantitative liquid storage part 129 is provided with a liquid dripping head 1211; the refrigeration outer cylinder 122 is fixedly connected with a spring limiting part 125 near the upper end part, a through hole is arranged in the middle of the spring limiting part 125, and a spring 126 is fixedly connected on the spring limiting part 125; the refrigerating outer cylinder 122 is connected with a liquid adding port 128; the push rod 123 penetrates through the upper end of the refrigeration outer cylinder 122 and the spring limiting part 125 to reach the lower end of the refrigeration outer cylinder 122, the lower end of the push rod 123 is connected with the piston 124, and the outer diameter of the piston 124 is equal to the inner diameter of the refrigeration outer cylinder 122; the upper part of the push rod 123 is fixedly connected with a spring pressing block 127;
the cell sap dropper 12 capable of being quantified has the working principle that: cell liquid is added into the refrigeration outer cylinder 122 from the liquid adding port 128, the push rod grab handle 121 is pressed, and the push rod 123, the spring pressing block 127 and the piston 124 are driven to move downwards together; the spring 126 is extruded and deformed, the piston 124 is pushed into the quantitative liquid storage part 129, the cell liquid starts to flow into the quantitative liquid storage part 129, when the quantitative liquid storage part 129 is filled with the cell liquid, the push rod handle 121 is released, the spring 126 is restored, the spring pressing block 127 and the push rod 123 are driven, the piston 124 faces upwards, and the piston 124 blocks the lower end of the refrigerating outer cylinder 122; the quantitative liquid storage portion 129 stores a quantitative amount of cell liquid; the quantitative liquid reservoir switch 1210 is controlled to allow the cell liquid to flow out from the dripper 1211.
Example 3
A use method of an assay sample processing device for hepatobiliary surgery comprises the following steps:
firstly, connecting a device power line 5 to a power supply;
secondly, cleaning the conveyor belt 3 to clean the surface, and placing the glass slide 9 on the conveyor belt 3 and right below the cell sap dropper 12, or padding a clean isolation pad under the glass slide 9;
thirdly, controlling a button 10 of the inoculation telescopic rod to drive the cell sap dropper 12 to descend to a proper height above the glass slide 9;
fourthly, pressing the push rod handle 121 part of the cell sap dropper 12 to make the cell sap in the refrigeration outer cylinder 122 enter the liquid storage part 129 filled with quantitative liquid, loosening the push rod handle 121 part to store the quantitative cell sap in the liquid storage part 129, and controlling the switch 1210 of the liquid storage part to make the cell drip in the middle of the glass slide 9;
fifthly, operating the conveyor belt control knob 7, moving the glass slide 9 under the scraping cotton coating head 17 on the conveyor belt 3, and automatically stopping the conveyor belt 3;
sixthly, controlling a button of a scraping and coating telescopic rod 13 to drive a scraping and coating cotton head 17 to descend to a proper height above the glass slide 9, and controlling a button of a scraping and coating transverse telescopic rod 15 to enable the scraping and coating transverse telescopic rod to stretch back and forth to scrape and coat cell sap uniformly;
seventhly, operating the conveyor belt control knob 7 to enable the conveyor belt 3 to continuously drive the glass slide 9 to rotate to the position right below the cover glass, and enabling the conveyor belt 3 to automatically stop;
eighthly, controlling a control button of a cover glass telescopic rod 18 to enable the cover glass to be lowered to a proper height above the glass slide 9 with the cover glass, controlling a button of a vacuum sucker telescopic rod 20 to lower one vacuum sucker telescopic rod 20 to enable one side of the cover glass to be attached to one side of the glass slide 9, and then enabling the other vacuum sucker telescopic rod 20 to be lowered to enable the cover glass to be slowly covered above the glass slide 9;
and ninthly, disconnecting the power of the vacuum suction cup 21 to ensure that the suction cup releases the cover glass.
Example 4
Referring to fig. 1 to 4, an assay sample processing device for hepatobiliary surgery includes: the cell culture device comprises a base 1, a control box 2, a conveyor belt 3, a conveyor belt rotating shaft 4, a power line 5, a PLC (programmable logic controller) 6, a conveyor belt control knob 7, a control button 8, a glass slide 9, an inoculation telescopic rod 10, an inoculation transverse rod 11, a cell sap dropper 12, a scraping telescopic rod 13, a scraping transverse rod 14, a scraping transverse telescopic rod 15, a scraping cotton head connecting rod 16, a scraping cotton head 17, a cover glass telescopic rod 18, a cover glass transverse rod 19, a vacuum sucker telescopic rod 20, a vacuum sucker 21 and a limit switch 22;
the base 1 is in a cuboid structure, the upper end face of the base is open, and the base is hollow; the right end face of the base 1 is connected with a control box 2; a conveyor belt rotating shaft 4 is respectively arranged at the left side and the right side in the base 1, and the conveyor belt 3 is connected to the conveyor belt rotating shaft 4; a power supply is arranged in the control box 2 and connected to each electromechanical component, and a power line 5 is led out from the inside and connected to the right side surface of the control box 2; a PLC (programmable logic controller) 6 is arranged in the control box 2; the upper surface of the control box 2 is connected with a conveyor belt control knob 7; the front of the control box 2 is provided with a control button 8 of each component; a slide glass 9 is placed on the conveyor belt 3; the left side of the back of the base 1 is fixedly connected with an inoculation telescopic rod 10, the head end of the inoculation telescopic rod 10 is fixedly connected with an inoculation cross rod 11, the head end of the inoculation cross rod 11 is provided with a through hole, and a cell sap dropper 12 is detachably connected in the through hole; a scraping telescopic rod 13 is fixedly connected to the middle of the back of the base 1, a scraping transverse rod 14 is fixedly connected to the head end of the scraping telescopic rod 13, a scraping transverse telescopic rod 15 is telescopically connected to the inside of the scraping transverse rod 14, a scraping cotton head connecting rod 16 is fixedly connected to the head end of the scraping transverse telescopic rod 15, and a scraping cotton head 17 is detachably connected to the lower end of the scraping cotton head connecting rod 16; the right side of the back of the base 1 is fixedly connected with a cover glass telescopic rod 18, the head end of the cover glass telescopic rod 18 is fixedly connected with a cover glass cross rod 19, the head end of the cover glass cross rod 19 is fixedly connected with two vacuum sucker telescopic rods 20, and the lower ends of the vacuum sucker telescopic rods 20 are connected with vacuum suckers 21; two ends of the upper surface of the base 1 are respectively provided with a limit switch 22, and the limit switches 22 are connected with the conveyor belt rotating shaft 4;
the PLC 6 is connected with a conveyor belt rotating shaft 4, the conveyor belt rotating shaft 4 is connected with a conveyor belt control knob 7 through a power cord 5, a limit switch 22 is connected with the conveyor belt rotating shaft 4, the conveyor belt control knob 7 is a master switch, a program is preset in the PLC 6, the conveyor belt rotating shaft 4 is controlled to rotate to a specific position and automatically stop, and the conveyor belt control knob 7 needs to be manually controlled to rotate; the position where the slide glass 9 stops on the conveyor belt 3 is just opposite to the position where the cotton head 17 and the cover glass are scraped; when the conveyor belt 3 drives the glass slide 9 to move to the position below the rightmost cover glass and stop, the conveyor belt control knob 7 is started again, and the conveyor belt rotating shaft 4 rotates to the left under the action of the right limit switch 22, so that the conveyor belt 3 is driven to rotate to the left;
three rows of control buttons 8 are vertically arranged and respectively correspond to three functions of inoculation, blade coating and cover glass control;
the vacuum sucker 21 sucks the glass slide when the power is on, and the glass slide falls when the power is off;
the scraping cotton head 17 is in a strip shape; the scrape coating cotton head 17 can be replaced, and the scrape coating is even guaranteed by the long-strip-shaped cotton head.
In conclusion, the laboratory sample processing device for hepatobiliary surgery is mechanized in structure, can perform inoculation, scraping and cover glass streamlined operation, only needs to manually perform button operation on each component, and does not need manual inoculation, scraping and cover glass; the quantitative cell sap dropper can ensure that the cell sap dripped on the glass slide is uniform every time, so that the phenomenon of nonuniform volume caused by manual inoculation is avoided, and the cell sap dropper adopts a refrigeration outer barrel, so that the cell sap in the cell sap dropper can be in a low-level environment, and the cell activity is kept; the automatic scraping function is matched with the strip-shaped scraping cotton head, so that cells can be uniformly scraped, and the uniformity of the cells cannot be ensured by manual scraping; the cover glass needs to be careful when in cover glass production, when a large number of cell culture samples are produced, the manual work load is increased by the manual cover glass, the manual work is saved by the automatic cover glass, and the cover glass can be accurately placed above the glass slide; the conveying belt controlled by the PLC can enable the glass slide driven by the conveying belt to stop at the same position every time, and therefore blade coating and accurate operation of cover glass are facilitated.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.
Claims (8)
1. An assay sample processing device for hepatobiliary surgery, comprising: the device comprises a base (1), a control box (2), a conveyor belt (3), a conveyor belt rotating shaft (4), a power line (5), a PLC (programmable logic controller) (6), a conveyor belt control knob (7), a control button (8), a glass slide (9), an inoculation telescopic rod (10), an inoculation transverse rod (11), a cell sap dropper (12), a scraping telescopic rod (13), a scraping transverse rod (14), a scraping transverse telescopic rod (15), a scraping cotton head connecting rod (16), a scraping cotton head (17), a cover glass telescopic rod (18), a cover glass transverse rod (19), a vacuum sucker telescopic rod (20) and a vacuum sucker (21);
the base (1) is in a cuboid structure, the upper end face of the base is open, and the base is hollow; the right end face of the base (1) is connected with a control box (2); a conveyor belt rotating shaft (4) is respectively arranged at the left side and the right side in the base (1), and the conveyor belt (3) is connected on the conveyor belt rotating shaft (4); a power supply is arranged in the control box (2) and connected to each electromechanical component, and a power line (5) is led out from the inside and connected to the right side surface of the control box (2); a PLC (programmable logic controller) controller (6) is arranged in the control box (2); the upper surface of the control box (2) is connected with a conveyor belt control knob (7); the front of the control box (2) is provided with a control button (8) of each component; a slide glass (9) is placed on the conveyor belt (3); the left side of the back of the base (1) is fixedly connected with an inoculation telescopic rod (10), the head end of the inoculation telescopic rod (10) is fixedly connected with an inoculation cross rod (11), the head end of the inoculation cross rod (11) is provided with a through hole, and a cell sap dropper (12) is detachably connected in the through hole; a scraping and coating telescopic rod (13) is fixedly connected to the middle of the back of the base (1), a scraping and coating transverse rod (14) is fixedly connected to the head end of the scraping and coating telescopic rod (13), a scraping and coating transverse telescopic rod (15) is telescopically connected to the inside of the scraping and coating transverse rod (14), a scraping and coating cotton head connecting rod (16) is fixedly connected to the head end of the scraping and coating transverse telescopic rod (15), and a scraping and coating cotton head (17) is detachably connected to the lower end of the scraping and coating cotton head connecting rod (16); the glass slide telescopic rod (18) is fixedly connected to the right side of the back of the base (1), the head end of the glass slide telescopic rod (18) is fixedly connected with a glass slide cross rod (19), the head end of the glass slide cross rod (19) is fixedly connected with two vacuum sucker telescopic rods (20), and the lower ends of the vacuum sucker telescopic rods (20) are connected with vacuum suckers (21).
2. The specimen processing device according to claim 1, wherein the PLC controller (6) is connected to the belt rotating shaft (4), the belt rotating shaft (4) is connected to the belt control knob (7) through the power cord (5), the PLC controller (6) is programmed in advance to control the belt rotating shaft (4) to rotate to a specific position to stop automatically, and the belt control knob (7) is controlled manually to rotate.
3. The specimen processing device for hepatobiliary surgery according to claim 1, wherein the control buttons (8) are vertically arranged in three rows, and respectively control the functions of inoculation, scraping and cover glass.
4. The specimen processing device for hepatobiliary surgery according to claim 1, wherein the vacuum chuck (21) is configured to hold the slide when powered on and to drop the slide when powered off.
5. The specimen processing device for hepatobiliary surgery according to claim 1, wherein the doctor blade cotton head (17) is elongated.
6. The specimen processing device for hepatobiliary surgery according to claim 1, wherein the pipette (12) comprises a plunger grip (121), a refrigerated outer cylinder (122), a plunger (123), a piston (124), a spring stopper (125), a spring (126), a spring pressing block (127), a filling port (128), a quantitative liquid storage part (129), a quantitative liquid storage part switch (1210), and a dropper (1211); the refrigerating outer cylinder (122) has refrigerating function, the lower end of the refrigerating outer cylinder is connected with a quantitative liquid storage part (129) and is controlled by a quantitative liquid storage part switch (1210), and the lower end of the quantitative liquid storage part (129) is provided with a liquid dropper (1211); the refrigeration outer cylinder (122) is fixedly connected with a spring limiting part (125) close to the upper end part, a through hole is formed in the middle of the spring limiting part (125), and a spring (126) is fixedly connected on the spring limiting part (125); a liquid adding opening (128) is connected to the refrigerating outer cylinder (122); the push rod (123) penetrates through the upper end of the refrigeration outer cylinder (122) and the spring limiting part (125) and reaches the lower end of the refrigeration outer cylinder (122), the lower end of the push rod (123) is connected with the piston (124), and the outer diameter of the piston (124) is equal to the inner diameter of the refrigeration outer cylinder (122); the upper part of the push rod (123) is fixedly connected with a spring pressing block (127).
7. A use method of an assay sample processing device for hepatobiliary surgery comprises the following steps:
firstly, connecting a device power line (5) to a power supply;
secondly, cleaning the conveyor belt (3) to clean the surface, and placing the glass slide (9) on the conveyor belt (3) and right below the cell sap dropper (12), or padding a clean isolation pad under the glass slide (9);
thirdly, controlling a button of an inoculation telescopic rod (10) to drive a cell sap dropper (12) to descend to a proper height above the glass slide (9);
fourthly, pressing a push rod handle (121) part of the cell sap dropper (12) to enable cell sap in the refrigeration outer cylinder (122) to enter a quantitative liquid storage part (129) filled with the cell sap, loosening the push rod handle (121) part, storing the quantitative cell sap in the quantitative liquid storage part (129), and controlling a quantitative liquid storage part switch (1210) to enable the cell sap to be dripped in the middle part of the glass slide (9);
fifthly, operating a conveyor belt control knob (7), moving the glass slide (9) under the scraping cotton head (17) on the conveyor belt (3), and automatically stopping the conveyor belt (3);
sixthly, controlling a control button of a scraping and coating telescopic rod (13) to drive a scraping and coating cotton head (17) to descend to a proper height above the glass slide (9), and controlling a scraping and coating transverse telescopic rod (15) button to stretch back and forth to uniformly scrape and coat the cell sap;
seventhly, operating a conveyor belt control knob (7) to enable the conveyor belt (3) to continuously drive the glass slide (9) to rotate to the position right below the cover glass, and enabling the conveyor belt (3) to automatically stop;
eighthly, controlling a control button of a cover glass telescopic rod (18) to enable the cover glass to be lowered to a proper height on the glass slide (9) with the cover glass, controlling a vacuum sucker telescopic rod (20) button to lower a vacuum sucker telescopic rod (20) to enable the cover glass to be attached to one side of the glass slide (9), and then enabling a vacuum sucker telescopic rod (20) to be lowered to enable the cover glass to be slowly covered above the glass slide (9);
and ninthly, disconnecting the power of the vacuum suction cup (21) to ensure that the suction cup loosens the cover glass.
8. According to any one of claims 1-7, there is disclosed a use of an assay sample processing device for hepatobiliary surgery in the field of medical sample assay.
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