CN109082377A - A kind of medicine and pharmacology cell culture apparatus - Google Patents
A kind of medicine and pharmacology cell culture apparatus Download PDFInfo
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- CN109082377A CN109082377A CN201811046458.5A CN201811046458A CN109082377A CN 109082377 A CN109082377 A CN 109082377A CN 201811046458 A CN201811046458 A CN 201811046458A CN 109082377 A CN109082377 A CN 109082377A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/40—Manifolds; Distribution pieces
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/26—Conditioning fluids entering or exiting the reaction vessel
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/14—Incubators; Climatic chambers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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Abstract
The invention discloses a kind of medicine and pharmacology cell culture apparatus, including device noumenon, door body, intelligent controller；It is divided into constant temperature incubation room and refrigerating chamber inside device noumenon, constant temperature incubation chamber interior is welded with first partition and constant temperature incubation room is divided into four floor culture chamber, every layer of culture chamber top is equipped with isocon, refrigerating chamber and constant temperature incubation room upper end middle position are bonded with intelligent controller, inner wall is uniformly provided with four cooling pieces from top to bottom on the right side of refrigerating chamber and constant temperature incubation room, and being connected by a hinge on the left of refrigerating chamber front end face right side, constant temperature incubation room front end face has door body；It can be to adding culture solution in the indoor culture dish of constant temperature incubation in the case where not opening door body, cell can be moved in refrigerating chamber and be saved after the completion of cell culture, and constant temperature incubation room, the indoor air of refrigeration can circulate, convenient for the growth and survival of cell.
Description
Technical field
The present invention relates to technical field of cell culture, specially a kind of medicine and pharmacology cell culture apparatus.
Background technique
Cell clone technology is also in cell culture, be a large amount of cell or its metabolite are obtained by cell culture, and
Because biological product is got by cell, cell culture technology is most crucial, most basic technology in biotechnology, carefully
Born of the same parents' culture needs dependent cells culture apparatus, and most of cell carries out aerobic respiration, needs to consume the air inside culture apparatus,
But existing part culture apparatus is not provided with air inlet in order to avoid the entrance of bacterium, and cell, which stops growing, to be eventually led to extremely
It dies, or is provided with air inlet, but without the air that purification enters, so that contamination of cells, causes cell culture to fail, trained in cell
In feeding process, cell may require that addition culture solution, usually all be, open the door of culture apparatus, and culture is instilled in culture dish
Liquid, this method will lead to bacterium and enter in culture apparatus, contamination of cells, and cell culture apparatus generally only cultivates cell
Function, can not store culture after cell, have a single function；To solve the above problems, now providing a kind of medicine and pharmacology cell training
Support device.
Summary of the invention
It, can be right in the case where not opening door body the object of the present invention is to provide a kind of medicine and pharmacology cell culture apparatus
Culture solution is added in the indoor culture dish of constant temperature incubation, cell can be moved in refrigerating chamber and be protected after the completion of cell culture
It deposits, and constant temperature incubation room, the indoor air of refrigeration can circulate, and convenient for the growth and survival of cell, solve in the prior art
Deficiency.
To achieve the above object, the invention provides the following technical scheme: a kind of medicine and pharmacology cell culture apparatus, including device
Ontology, door body, intelligent controller；The inside of described device ontology is divided into constant temperature incubation room and refrigerating chamber, and constant temperature incubation room is set to
Constant temperature incubation room there are three first partition and is divided into four floor culture chamber by the left side of refrigerating chamber, the welding of constant temperature incubation chamber interior, point
Not Wei first layer culture chamber, second layer culture chamber, third layer culture chamber, the 4th layer of culture chamber, the top of every layer of culture chamber is viscous
It is connected to isocon, isocon is in lower section, the constant temperature incubation ceiling portion by-level position of first partition, each first partition
Between lengthwise position, constant temperature incubation room top center lengthwise position offer through-hole, per two adjacent through-holes between be inserted in
There is protective jacket, the first diversion pipe, the second diversion pipe, third diversion pipe, the 4th diversion pipe, the first water conservancy diversion have been inserted in protective jacket
Pipe, the second diversion pipe are respectively arranged on the front of third diversion pipe, the 4th diversion pipe, and it is left that the first diversion pipe is set to the second diversion pipe
End, the first diversion pipe, the second diversion pipe, third diversion pipe, the 4th diversion pipe top both pass through constant temperature incubation room top center
The circular hole of lengthwise position is connected with wort cylinder, and supporting block, supporting block weldering are welded at left and right sides of the wort cylinder bottom
It connects at the top of device noumenon left end, the first diversion pipe is connected to the isocon on first layer culture chamber top, the second diversion pipe and
The isocon connection on two layers of culture chamber top, third diversion pipe are connected to the isocon on third layer culture chamber top, the 4th water conservancy diversion
The isocon on the tetra- layers of culture chamber top Guan Yu is connected to, and constant temperature incubation room lower end rear middle position offers the first air inlet
Mouthful, the first air inlet rear is equipped with the first air purifier A, the first air purifier A and is adhered to device noumenon rear, and first is empty
The air inlet end of gas purifier A is connected with the first exhaust fan, and constant temperature incubation room top left hand offers air outlet A, on air outlet A
End is connected with the first air purifier B, the first air purifier B and is welded in device noumenon top left hand, the first air purifier B
Upper end offers the first air outlet；The refrigerating chamber and constant temperature incubation room upper end middle position are bonded with intelligent controller, refrigerate
Inner wall is uniformly provided with four cooling pieces, refrigerating chamber front end face right side, constant temperature incubation room from top to bottom on the right side of room and constant temperature incubation room
Being connected by a hinge on the left of front end face has door body；
There are three second partitions, second partition to be classified as four layers of preservation chamber, every layer of preservation chamber, every layer for welding in the refrigerating chamber
It is equipped with pallet at left and right sides of culture chamber bottom, is placed with culture dish in pallet, every layer of right side for saving chamber, every layer of culture chamber
It is bonded with cooling piece among inner wall, refrigerating chamber lower end rear middle position offers the second air inlet, after the second air inlet
Side is equipped with the second air purifier A, the second air purifier A and is adhered to device noumenon rear, the air inlet of the second air purifier A
Mouth end is connected with the second exhaust fan, and refrigerating chamber top right side opening is equipped with air outlet B, and it is net that the upper end air outlet B is connected with the second air
Change device B, the second air purifier B is welded on the right side of device noumenon top, and the second upper end air purifier B offers the second outlet air
Mouthful.
Preferably, it is equipped with automatic temperature control system inside the intelligent controller, is equipped on the left of the front end face of intelligent controller
Left display screen, is equipped with right display screen on the right side of the front end face of intelligent controller, left display screen, right display screen lower section be equipped with constant temperature training
Room power switch, refrigerating chamber power switch are supported, constant temperature incubation room power switch is set on the left of refrigerating chamber power switch, constant temperature incubation
Room power switch, refrigerating chamber power switch lower section be equipped with operation button plate, one end of intelligent controller is divided by conducting wire connection
Other cooling piece, the first exhaust fan, the second exhaust fan, the first air purifier A, the first air purifier B, the second air purifier
A, the second air purifier B is connected using parallel way between cooling piece, and one end of intelligent controller is connected with electricity by conducting wire
Source connector.
Preferably, the door body includes doorframe, interior glass door, gap, outer glass door, handle, and doorframe is rectangular box-like knot
Structure, interior glass door are embedded in and are bonded on the inside of doorframe, and interior glass door and refrigerating chamber, constant temperature incubation room are bonded with rectangle at opening face
Sealing ring, outer glass door are embedded in and are bonded on the outside of doorframe, and the side of outer glass door folding is Nian Jie with handle, interior glass door with outside
There are gaps between glass door.
Preferably, the right side opening of the wort cylinder upper end is equipped with inlet, the first water conservancy diversion of wort cylinder bottom connection
Pipe, the second diversion pipe, third diversion pipe, the 4th diversion pipe upper end integrally formed with drop pot, drip pot top be equipped with flow velocity
Adjuster.
Preferably, left side dropper, the culture of the lower end face of left side dropper below have been inserted on the left of the isocon
Ware has been inserted in right side dropper, the culture dish of the lower end face of right side dropper below on the right side of isocon.
Preferably, it is bonded with the second insulating layer on the inner wall of the refrigerating chamber, is bonded on the inner wall of constant temperature incubation room
One insulating layer.
Preferably, the inner wall of the refrigerating chamber, the inner wall of constant temperature incubation room, first partition, second partition are all made of sterile
Plate is made.
Part is not directed in the device to be the same as those in the prior art or can be realized by using the prior art.
Compared with prior art, beneficial effects of the present invention are as follows: a kind of medicine and pharmacology cell culture apparatus, are equipped with constant temperature and train
Room and refrigerating chamber are supported, cell can be moved in refrigerating chamber after the completion of cell culture and be saved, it is thin after avoiding culture
Bacterium is carrying out using preceding contaminated and death, and pallet is convenient for the mobile culture dish of culture dish and is not contacted with culture dish；First takes out
Outside air is pumped into constant temperature incubation room and refrigerating chamber by blower, the second exhaust fan, the first air purifier A, the second air
Clarifier A purifies bacterium and harmful substance in the air being pumped by the first exhaust fan, the second exhaust fan, avoids pollution cell,
The air of first air purifier B, the second air purifier B purification discharge, avoids having in constant temperature incubation room and refrigerating chamber
Evil substance is discharged to outside contamination environment, and constant temperature incubation room, the indoor air of refrigeration can circulate, convenient for cell growth and deposit
It is living；First diversion pipe, the second diversion pipe, third diversion pipe, the 4th diversion pipe, isocon, left side dropper, right side dropper, drop pot,
The setting of flow regulator can need to instill culture solution by drop pot, flow regulator when culture solution to control in culture dish
Amount, can avoid the dirt of bacterium to culture solution is added in the indoor culture dish of constant temperature incubation in the case where not opening door body
Dye；Doorframe is set as double glazing door, when can check convenient for staff, additionally it is possible to play the role of thermal insulation, rectangle is close
Seal ring enough seals door body and constant temperature incubation room, refrigerating chamber, avoids the entrance of bacterium；The inner wall of refrigerating chamber, constant temperature incubation room it is interior
Wall, first partition, second partition, which are all made of sterile plate and are made, can effectively avoid the pollution of bacterium；Left display screen, the right side
The temperature that display screen shows constant temperature incubation room respectively, refrigeration indoor temperature and need to adjust, automatic temperature control system automatic adjustment temperature
Degree.
Detailed description of the invention
Fig. 1 is a kind of positive overall structure cross-sectional view of medicine and pharmacology cell culture apparatus；
Fig. 2 is a kind of door body structure cross-sectional view of medicine and pharmacology cell culture apparatus；
Fig. 3 is a kind of intelligent controller structure schematic diagram of medicine and pharmacology cell culture apparatus.
Figure label explanation: 1- device noumenon, 2- constant temperature incubation room, 3- first partition, the first insulating layer of 4-, 5- culture
Ware, 6- pallet, the left side 7- dropper, the first air outlet of the first air purifier of 8- B, 9-, 10- supporting block, 11- wort cylinder, 12-
First diversion pipe, the second diversion pipe of 13-, 14- inlet, 15- isocon, dropper, 17- intelligent controller, 18- the on the right side of 16-
Two air outlets, 19- the second air purifier B, 20- cooling piece, 21- second partition, the second insulating layer of 22-, 23- refrigerating chamber, 24-
Second air inlet, the first air inlet of 25-, 26- doorframe, glass door in 27-, the gap 28-, the outer glass door of 29-, the left display screen of 30-,
The right display screen of 31-, 32- constant temperature incubation room power switch, 33- refrigerating chamber power switch, 34- operation button plate.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments, is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
In the description of the present invention, it is to be understood that, term " on ", "lower", "front", "rear", "left", "right", "top",
The orientation or positional relationship of the instructions such as "bottom", "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings, merely to just
In description the present invention and simplify description, rather than the device or element of indication or suggestion meaning must have a particular orientation, with
Specific orientation construction and operation, therefore be not considered as limiting the invention.
Please refer to Fig. 1-3, a kind of medicine and pharmacology cell culture apparatus, including device noumenon 1, door body, intelligent controller 17；Dress
The inside for setting ontology 1 is divided into constant temperature incubation room 2 and refrigerating chamber 23, and the first insulating layer 4 is bonded on the inner wall of constant temperature incubation room 2,
For keeping the temperature, constant temperature incubation room 2 is set to the left side of refrigerating chamber 23, and first partition 3 that there are three 2 interior welds of constant temperature incubation room simultaneously will
Constant temperature incubation room 2 is divided for four floor culture chamber, respectively first layer culture chamber, second layer culture chamber, third layer culture chamber, the 4th layer
Culture chamber, convenient for placing the culture dish 5 of more culture cells, the top of every layer of culture chamber is bonded with isocon 15, is convenient for
Left side dropper 7, the culture dish of the lower end face of left side dropper 7 below have been inserted in the shunting of culture solution, the left side of isocon 15
5, convenient for adding culture solution into culture dish 5, right side dropper 16 has been inserted on the right side of isocon 15, and the lower of right side dropper 16 is rectified
To culture dish 5 below, convenient for adding culture solution into culture dish 5, isocon 15 is set to the lower section of first partition 3, constant temperature
2 top center horizontal position of culturing room, the intermediate lengthwise position of each first partition 3,2 top center lengthwise position of constant temperature incubation room
Through-hole is offered, protective jacket has been inserted between every two adjacent through-holes, for protecting the first diversion pipe 12, the second water conservancy diversion
Pipe 13, third diversion pipe, the 4th diversion pipe have been inserted in the first diversion pipe 12, the second diversion pipe 13, third water conservancy diversion in protective jacket
Pipe, the 4th diversion pipe, the first diversion pipe 12, the second diversion pipe 13 are respectively arranged on the front of third diversion pipe, the 4th diversion pipe, the
One diversion pipe 12 is set to 13 left end of the second diversion pipe, the first diversion pipe 12, the second diversion pipe 3, third diversion pipe, the 4th diversion pipe
Top both pass through the circular hole of 2 top center lengthwise position of constant temperature incubation room and be connected with wort cylinder 11,11 upper end of wort cylinder
Right side opening be equipped with inlet 14,11 bottom of wort cylinder connection the first diversion pipe 12, the second diversion pipe 13, third water conservancy diversion
It manages, the upper end of the 4th diversion pipe integrally formed with drop pot, drips the top of pot equipped with flow regulator, drips pot, flow regulator
Control the amount for instilling culture solution, it can be to being added in the culture dish 5 in constant temperature incubation room 2 in the case where not opening door body
Culture solution avoids the pollution of bacterium, is welded with supporting block 10 at left and right sides of 11 bottom of wort cylinder, supporting block 10 is welded on dress
It sets at the top of 1 left end of ontology, the first diversion pipe 12 is connected to the isocon 15 on first layer culture chamber top, the second diversion pipe 13 and the
The isocon 15 on two layers of culture chamber top is connected to, and third diversion pipe is connected to the isocon 15 on third layer culture chamber top, and the 4th
Diversion pipe is connected to the isocon 15 on the 4th layer of culture chamber top, and 2 lower end rear middle position of constant temperature incubation room offers first
Air inlet 25,25 rear of the first air inlet is equipped with the first air purifier A, after the first air purifier A is adhered to device noumenon 1
Side, extraneous air of evolving, the air inlet end of the first air purifier A are connected with the first exhaust fan, and 2 top of constant temperature incubation room is left
Side offers air outlet A, and the upper end air outlet A is connected with the first air purifier B8, for extraneous air of evolving, purifies constant temperature
The air that culturing room 2 is discharged, the first air purifier B8 are welded in 1 top left hand of device noumenon, on the first air purifier B8
End offers the first air outlet 9；Refrigerating chamber 23 and 2 upper end middle position of constant temperature incubation room are bonded with intelligent controller 17, intelligence
It is equipped with automatic temperature control system inside controller 17, is equipped with left display screen 30, intelligent control on the left of the front end face of intelligent controller 17
Be equipped with right display screen 31 on the right side of the front end face of device 17, left display screen 30, right display screen 31 lower section be equipped with constant temperature incubation room power supply
Switch 32, refrigerating chamber power switch 33, left display screen 30, right display screen 31 show constant temperature incubation room 2, in refrigerating chamber 23 respectively
Temperature and the temperature that need to be adjusted, automatic temperature control system automatically adjust temperature, and constant temperature incubation room power switch 32 is set to refrigerating chamber electricity
The left side of source switch 33, constant temperature incubation room power switch 32, refrigerating chamber power switch 33 lower section be equipped with operation button plate 34, intelligence
One end of controller 17 by conducting wire connect respectively cooling piece 20, the first exhaust fan, the second exhaust fan, the first air purifier A,
First air purifier B8, the second air purifier A, the second air purifier B19 are connected using parallel way between cooling piece 20
It connects, one end of intelligent controller 17 is connected with power connection by conducting wire；Refrigerating chamber 23 is with 2 right side inner wall of constant temperature incubation room by upper
Four cooling pieces 20 are uniformly provided under, for freezing, on the left of 23 front end face of refrigerating chamber right side, 2 front end face of constant temperature incubation room
Being connected by a hinge has door body, and door body includes doorframe 26, interior glass door 27, gap 28, outer glass door 29, handle, and doorframe 26 is
Rectangular box-like structure, interior glass door 27 are embedded in and are bonded in 26 inside of doorframe, interior glass door 27 and refrigerating chamber 23, constant temperature incubation room
It is bonded with rectangular seal ring at 2 opening faces, convenient for sealing door body and constant temperature incubation room 2, refrigerating chamber 23, the outer insertion of glass door 29 is simultaneously
It is bonded in 26 outside of doorframe, the side that outer glass door 29 opens and closes is Nian Jie with handle, convenient for opening door body, interior glass door 27, gap
28, outer glass door 29, convenient for watching the situation of cell, there are gap 28 between interior glass door 27 and outer glass door 29, enhancing every
Temp effect；
The second insulating layer 22 is bonded on the inner wall of refrigerating chamber 23, for keeping the temperature, there are three second partitions for welding in refrigerating chamber 23
21, second partition 21 is classified as four layers of preservation chamber, corresponding with the culture chamber in constant temperature incubation room 2, saves culture convenient for depositing
Cell afterwards is equipped with pallet 6 at left and right sides of every layer of preservation chamber, every layer of culture chamber bottom, culture dish 5 is placed in pallet 6,
It is bonded with cooling piece 20 among every layer of right side inner wall for saving chamber, every layer of culture chamber, for freezing, in 23 lower end rear of refrigerating chamber
Between position offer the second air inlet 24,24 rear of the second air inlet is equipped with the second air purifier A, purify extraneous air,
Bacterium and harmful substance is avoided to enter in refrigerating chamber 23, the second air purifier A is adhered to 1 rear of device noumenon, the second air
The air inlet end of clarifier A is connected with the second exhaust fan, and 23 top right side opening of refrigerating chamber is equipped with air outlet B, and the upper end air outlet B connects
It is connected to the second air purifier B19, the air being discharged in purification refrigerating chamber 23, the second air purifier B19 is welded in device sheet
On the right side of 1 top of body, the second upper end air purifier B19 offers the second air outlet 18；The inner wall of refrigerating chamber 23, constant temperature incubation room
2 inner wall, first partition 3, second partition 21 are all made of sterile plate and are made, and reduce the growth of bacterium, avoid germ contamination.
Working principle: the present invention in constant temperature incubation room 2 before use, powering on by power connection, passes through constant temperature incubation
Room power switch 32 opens the first air purifier A, the first air purifier B8, the first exhaust fan, perseverance in constant temperature incubation room 2
Cooling piece 20 in warm culturing room 2, by the temperature in 34 regulating thermostatic culturing room 2 of operation button plate to suitable temperature value,
Automatic temperature control system automatically adjusts the temperature in it, is disappeared by disinfectant to the inside of constant temperature incubation room 2,6 entirety of pallet
Pallet 6 is put by poison sterilizing, constant temperature incubation room 2 in use, by being put into pallet 6 equipped with the culture dish 5 of cell to be cultivated
It is cultivated in culture chamber, closing door body cultivates cell in the environment of constant temperature, and the mistake of cell culture can be checked by door body
Journey and degree, if the culture solution in culture dish 5 can pour into suitable culture solution to wort cylinder 11 by inlet 14 when less
It is interior, it is controlled by drop pot, flow regulator and adjusts the culture solution being added dropwise in each culture dish 5, for the growth of cell, carefully
After born of the same parents cultivate, the related culture dish 5 of cultured cell and pallet 6 are moved in refrigerating chamber 23；After 2 use of constant temperature incubation room
It is carried out disinfection sterilizing by inside of the disinfectant to constant temperature incubation room 2, closes constant temperature incubation room power switch 32, it is empty to disconnect first
Gas purifier A, the first air purifier B8, the first exhaust fan, cooling piece 20 in constant temperature incubation room 2 power supply, shut door body
?；
Refrigerating chamber 23 is before use, open the second air purifier A in refrigerating chamber 23, second by refrigerating chamber power switch 33
Air purifier B19, the second exhaust fan, the cooling piece 20 in refrigerating chamber 23, are adjusted in refrigerating chamber 23 by operation button plate 34
Temperature to suitable temperature value, automatic temperature control system automatically adjusts the temperature in it, by disinfectant in refrigerating chamber 23
Portion carries out disinfection sterilizing, then the related culture dish 5 of cultured cell and pallet 6 are moved in refrigerating chamber 23, and closing door body makes
Cell stores in the environment of constant low temperature, and the preservation state of cell culture can be checked by door body, leads to after 23 use of refrigerating chamber
It crosses disinfectant to carry out disinfection sterilizing to the inside of refrigerating chamber 23, closes refrigerating chamber power switch 33, disconnect the second air purifier
A, the power supply of the second air purifier B19, the second exhaust fan, cooling piece 2 in refrigerating chamber 23, shuts door body；
If constant temperature incubation room 2, refrigerating chamber 23 are when not in use, the connection of power interface and power supply can be disconnected, that is, disconnects intelligence control
The power supply of device 17 processed, has closed door body.
The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention, the technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention, the claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (7)
1. a kind of medicine and pharmacology cell culture apparatus, including device noumenon (1), door body, intelligent controller (17)；It is characterized by:
The inside of described device ontology (1) is divided into constant temperature incubation room (2) and refrigerating chamber (23), and constant temperature incubation room (2) are set to refrigerating chamber
(23) left side, constant temperature incubation room (2) interior welds are divided into the training of four floor there are three first partition (3) and by constant temperature incubation room (2)
Support chamber, respectively first layer culture chamber, second layer culture chamber, third layer culture chamber, the 4th layer of culture chamber, the top of every layer of culture chamber
End is bonded with isocon (15), and isocon (15) is set to the lower section of first partition (3), constant temperature incubation room (2) top center water
Prosposition is set, and the intermediate lengthwise position of each first partition (3), constant temperature incubation room (2) top center lengthwise position offer through-hole,
It has been inserted in protective jacket between per two adjacent through-holes, the first diversion pipe (12), the second diversion pipe have been inserted in protective jacket
(13), third diversion pipe, the 4th diversion pipe, the first diversion pipe (12), the second diversion pipe (13) are respectively arranged on third diversion pipe,
The front of four diversion pipes, the first diversion pipe (12) are set to the second diversion pipe (13) left end, the first diversion pipe (12), the second diversion pipe
(13), third diversion pipe, the 4th diversion pipe top both pass through constant temperature incubation room (2) top center lengthwise position circular hole connection
Have wort cylinder (11), is welded with supporting block (10) at left and right sides of wort cylinder (11) bottom, supporting block (10) welding
At the top of device noumenon (1) left end, the first diversion pipe (12) is connected to the isocon (15) on first layer culture chamber top, and second leads
Flow tube (13) is connected to the isocon (15) on second layer culture chamber top, the shunting of third diversion pipe and third layer culture chamber top
(15) connection is managed, the 4th diversion pipe is connected to the isocon (15) on the 4th layer of culture chamber top, constant temperature incubation room (2) lower end
Rear middle position offers the first air inlet (25), and the first air inlet (25) rear is equipped with the first air purifier A, and first is empty
Gas purifier A is adhered to device noumenon (1) rear, and the air inlet end of the first air purifier A is connected with the first exhaust fan, constant temperature
Culturing room (2) top left hand offers air outlet A, and the upper end air outlet A is connected with the first air purifier B(8), the first air is net
Change device B(8) it is welded in device noumenon (1) top left hand, the first air purifier B(8) upper end offers the first air outlet (9)；
The refrigerating chamber (23) and constant temperature incubation room (2) upper end middle position are bonded with intelligent controller (17), refrigerating chamber (23) and perseverance
Inner wall is uniformly provided with four cooling pieces (20), refrigerating chamber (23) front end face right side, constant temperature training from top to bottom on the right side of warm culturing room (2)
It supports to be connected by a hinge on the left of room (2) front end face and has door body；
There are three second partition (21), second partition (21) to be classified as four layers of preservation chamber for welding in the refrigerating chamber (23), often
It is equipped with pallet (6), is placed with culture dish (5) in pallet (6), every layer at left and right sides of layer preservation chamber, every layer of culture chamber bottom
It saves and is bonded with cooling piece (20) among the right side inner wall of chamber, every layer of culture chamber, refrigerating chamber (23) lower end rear interposition
It sets and offers the second air inlet (24), the second air inlet (24) rear is equipped with the second air purifier A, the second air purifier A
It is adhered to device noumenon (1) rear, the air inlet end of the second air purifier A is connected with the second exhaust fan, refrigerating chamber (23) top
Right side opening is held to be equipped with air outlet B, the upper end air outlet B is connected with the second air purifier B(19), the second air purifier B(19)
It is welded on the right side of device noumenon (1) top, the second air purifier B(19) upper end offers the second air outlet (18).
2. a kind of medicine and pharmacology cell culture apparatus according to claim 1, it is characterised in that: the intelligent controller (17)
Inside is equipped with automatic temperature control system, is equipped with left display screen (30) on the left of the front end face of intelligent controller (17), intelligent controller
(17) right display screen (31) are equipped on the right side of front end face, left display screen (30), right display screen (31) lower section be equipped with constant temperature incubation
Room power switch (32), refrigerating chamber power switch (33), constant temperature incubation room power switch (32) are set to refrigerating chamber power switch
(33) left side, constant temperature incubation room power switch (32), refrigerating chamber power switch (33) lower section be equipped with operation button plate (34), intelligence
The one end that can control device (17) is net by conducting wire connection difference cooling piece (20), the first exhaust fan, the second exhaust fan, the first air
Change device A, the first air purifier B(8), the second air purifier A, the second air purifier B(19), cooling piece is adopted between (20)
It is connected with parallel way, one end of intelligent controller (17) is connected with power connection by conducting wire.
3. a kind of medicine and pharmacology cell culture apparatus according to claim 1, it is characterised in that: the door body includes doorframe
(26), interior glass door (27), gap (28), outer glass door (29), handle, doorframe (26) are rectangular box-like structure, interior glass door
(27) it is embedded in and is bonded on the inside of doorframe (26), interior glass door (27) and refrigerating chamber (23), constant temperature incubation room (2) opening are glued at face
Be connected to rectangular seal ring, outer glass door (29), which is embedded in, to be simultaneously bonded on the outside of doorframe (26), the side of outer glass door (29) folding with
Handle bonding, there are gaps between interior glass door (27) and outer glass door (29).
4. a kind of medicine and pharmacology cell culture apparatus according to claim 1, it is characterised in that: on the wort cylinder (11)
The right side opening at end be equipped with inlet (14), wort cylinder (11) bottom connection the first diversion pipe (12), the second diversion pipe (13),
Third diversion pipe, the 4th diversion pipe upper end integrally formed with drop pot, drip pot top be equipped with flow regulator.
5. a kind of medicine and pharmacology cell culture apparatus according to claim 1, it is characterised in that: a left side for the isocon (15)
Left side dropper 9 has been inserted in side, and (culture dish (5) of lower end face below, isocon have been inserted in left side dropper on the right side of (15)
Right side dropper (16), the culture dish (5) of the lower end face of right side dropper (16) below.
6. a kind of medicine and pharmacology cell culture apparatus according to claim 1, it is characterised in that: the refrigerating chamber (23) it is interior
It is bonded on wall the second insulating layer (4), the first insulating layer (22) is bonded on the inner wall of constant temperature incubation room (2).
7. a kind of medicine and pharmacology cell culture apparatus according to claim 1, it is characterised in that: the refrigerating chamber (23) it is interior
Wall, the inner wall of constant temperature incubation room (2), first partition (3), second partition (22) are all made of sterile plate and are made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811046458.5A CN109082377A (en) | 2018-09-08 | 2018-09-08 | A kind of medicine and pharmacology cell culture apparatus |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811046458.5A CN109082377A (en) | 2018-09-08 | 2018-09-08 | A kind of medicine and pharmacology cell culture apparatus |
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| CN109082377A true CN109082377A (en) | 2018-12-25 |
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| CN201811046458.5A Pending CN109082377A (en) | 2018-09-08 | 2018-09-08 | A kind of medicine and pharmacology cell culture apparatus |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111257080A (en) * | 2020-02-29 | 2020-06-09 | 右江民族医学院附属医院 | Assay sample processing apparatus for hepatobiliary surgery |
| CN111374456A (en) * | 2018-12-27 | 2020-07-07 | 云南全控机电有限公司 | Culture dish storage cabinet with nitrogen |
| CN112251325A (en) * | 2020-11-03 | 2021-01-22 | 深圳市知猪互联科技有限公司 | Screening equipment for microbial fertilizer producing bacteria and fermentation process thereof |
| CN113481086A (en) * | 2021-06-11 | 2021-10-08 | 新疆维吾尔自治区人民医院 | Anti-pollution anaerobic bacteria culture device for clinical laboratory and operation method thereof |
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2018
- 2018-09-08 CN CN201811046458.5A patent/CN109082377A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111374456A (en) * | 2018-12-27 | 2020-07-07 | 云南全控机电有限公司 | Culture dish storage cabinet with nitrogen |
| CN111257080A (en) * | 2020-02-29 | 2020-06-09 | 右江民族医学院附属医院 | Assay sample processing apparatus for hepatobiliary surgery |
| CN111257080B (en) * | 2020-02-29 | 2023-03-28 | 右江民族医学院附属医院 | Assay specimen processing device for hepatobiliary surgery |
| CN112251325A (en) * | 2020-11-03 | 2021-01-22 | 深圳市知猪互联科技有限公司 | Screening equipment for microbial fertilizer producing bacteria and fermentation process thereof |
| CN112251325B (en) * | 2020-11-03 | 2021-08-27 | 沈阳丰恒泰农业科技有限公司 | Microbial fertilizer produces fungus screening installation |
| CN113481086A (en) * | 2021-06-11 | 2021-10-08 | 新疆维吾尔自治区人民医院 | Anti-pollution anaerobic bacteria culture device for clinical laboratory and operation method thereof |
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