CN113481086A

CN113481086A - Anti-pollution anaerobic bacteria culture device for clinical laboratory and operation method thereof

Info

Publication number: CN113481086A
Application number: CN202110656957.1A
Authority: CN
Inventors: 杨丽玮; 王玲玲; 卢佩佩; 庞秀慧; 贾海英
Original assignee: Peoples Hospital of Xinjiang Uygur Autonomous Region
Current assignee: Peoples Hospital of Xinjiang Uygur Autonomous Region
Priority date: 2021-06-11
Filing date: 2021-06-11
Publication date: 2021-10-08

Abstract

An anti-pollution type bacteria culture device for a clinical laboratory and an operation method thereof, the anti-pollution type anaerobic bacteria culture device for the clinical laboratory comprises an incubator, an operation display unit, a purification unit, a roller, a push-pull handle, a nitrogen supply unit, a reagent adding unit and a bacteria culture device control system, and a method of introducing nitrogen and exhausting air is adopted, can effectively solve the problems that the anaerobic condition of anaerobic bacteria culture can not be effectively ensured and is easy to be interfered by external factors in the prior art, the edge of the observation window is embedded with a sealing rubber strip, can prevent anaerobic bacteria from being easily polluted in the growth process, can better control the growth environment of the anaerobic bacteria by controlling the temperature and controlling the content of metabolite carbon dioxide of the anaerobic bacteria, promotes the formation of anaerobic bacterial colonies, and simultaneously, in the process of culturing the anaerobic bacteria, the adverse effect of manually and directly operating the culture dish is reduced to the maximum extent.

Description

Anti-pollution anaerobic bacteria culture device for clinical laboratory and operation method thereof

Technical Field

The invention relates to the technical field of disinfection and sterilization of clinical laboratory, in particular to an anti-pollution anaerobic bacteria culture device of clinical laboratory and an operation method thereof.

Background

The clinical laboratory includes many branches, such as clinical medicine, clinical microbiology, immunology, blood detection, body fluid detection, and bacterial culture, and each branch plays a role in analyzing and processing various patient samples. The bacteria culture is to culture collected bacteria sample in culture medium under certain conditions, to make bacteria grow and propagate by artificial method, to select culture method and culture medium according to bacteria type and purpose, to set culture conditions (temperature, pH value, time, oxygen demand, etc.). The general operation steps are that the specimen is firstly inoculated on a solid culture medium for separation culture. And further performing morphological, biochemical and serological reaction identification on the obtained single colony. The culture medium is usually prepared into liquid, semisolid, solid and the like by beef soup, peptone, sodium chloride, glucose, blood and the like and special substances required by certain bacteria. The general bacteria can grow in the presence of oxygen at 37 ℃ for 18-24 hours. Anaerobic bacteria need to grow after being placed in an anaerobic environment for 2-3 days, and the culture of individual bacteria such as tubercle bacillus needs 1 month. In the process of bacterial culture, the method deals with a plurality of bacteria, viruses and growth environments, and is extremely "critical" to the growth environments, such as culture medium, temperature, humidity, aerobic or anaerobic, if the growth conditions cannot be controlled, the bacterial culture is unsuccessful, and the test fails. In the prior art, the bacterial culture of the clinical laboratory is mostly the culture of aerobic bacteria, and the study on the culture of anaerobic bacteria does not arouse the attention of medical workers and medical researchers.

Anaerobic bacteria are important components of normal flora of human body, are widely present on the deep mucosal surface of skin and cavity of human body, and cause the oxygen concentration of local tissues to be reduced under the condition of tissue ischemia, necrosis or aerobic bacterial infection, so that the anaerobic bacterial infection is generated. Especially in recent years, anaerobic bacteria infection is more and more emphasized by clinical laboratory doctors, especially some medical scientific research institutions, and in clinical surgical infection cases, the detection rate of anaerobic bacteria is at least more than 50%. According to the data of Zhongshan hospital, the detection rate of anaerobic bacteria in abdominal infection is 60.67%, and the detection rate accounts for 70.58% of appendiceal abscess and incision suppuration after appendicectomy. Anaerobic bacteria not only cause severe thoraco-abdominal infections and abscesses, but many severe soft tissue necrotizing infections are almost exclusively associated with anaerobic bacteria. Therefore, how to create a better hypoxic environment, and rapidly and efficiently culture the anaerobic bacteria colonies with considerable colonies under the condition of hypoxia or even no oxygen becomes a technical problem which needs to be solved urgently by the technical personnel in the field.

In clinical laboratory, medical staff usually need to carry out the bacterial culture work to the body fluid of the patient who gathers, urine sample or secretion, and the cultivation that contains anaerobe, usually need add medicament or sample observation in the process of cultivating, and this just leads to the bacterial culture process can receive the influence of external environment to reduce the accuracy of bacterial culture result, culture efficiency is lower.

In addition, the temperature has great influence on the culture of anaerobic bacteria, and the bacteria can be classified into Psychrophiles (Psychrophiles) according to the temperature suitable for the growth of the bacteria, and the optimal growth temperature is (10-20 ℃); mesophilic bacteria (Mesophiles) at 20-40 deg.C; thermophilic bacteria (Thermophiles) grow best up to 56-60 ℃. Pathogenic bacteria are mesophilic bacteria, the temperature of mesophilic anaerobe is generally between 30-40 ℃, and in the actual culture process, the temperature of human body temperature, namely 37 ℃, is generally taken for bacterial culture. Therefore, in the clinical laboratory, it is important to maintain the temperature condition suitable for anaerobic bacteria growth during the process of culturing bacteria.

Disclosure of Invention

The invention aims to provide an anti-pollution anaerobic bacteria culture device for a clinical laboratory and an operation method thereof, and aims to solve the technical problems that anaerobic conditions for anaerobic bacteria culture cannot be effectively guaranteed, the anaerobic conditions are easily interfered by external factors, anaerobic bacterial colonies are easily polluted in the growth process, and the temperature cannot be effectively adjusted in the prior art.

In order to achieve the above purpose, the invention provides the following technical scheme:

the utility model provides an anti-pollution formula anaerobe culture apparatus of clinical laboratory, includes incubator, operation display element, purification unit, gyro wheel, push-and-pull handle, supplies nitrogen unit, reagent interpolation unit and bacteria culture apparatus control system. The upper part of the incubator is connected with the operation display unit, one side of the incubator is fixedly connected with the purification unit, one side of the incubator is fixedly connected with a door-shaped push-pull handle, and the bottom of the incubator is provided with a plurality of rollers; one side of incubator is put the fixed nitrogen unit that supplies of placing of fixed station through holding, supply the nitrogen unit to include nitrogen gas holder, gas-supply pipe and be located the control valve on the gas-supply pipe, be provided with a plurality of rooms of cultivateing, every in the incubator cultivate the room all with purification unit gas circuit intercommunication, every cultivate indoor all being provided with reagent interpolation unit, purification unit, control valve, operation display unit all are connected with bacteria cultivation device control system electricity.

Every the culture room all has the rectangle viewing aperture that forms on the incubator panel, one side of rectangle viewing aperture articulates there is the observation window, the observation window adopts transparent double-layer material to make, the edge of observation window inlays and is equipped with joint strip for when the observation window lock is on the rectangle viewing aperture, seal up the lock gap.

The control unit of the bacteria culture device is arranged at the top of the incubator and is electrically connected with an external power supply, the control unit of the bacteria culture device and the operation display unit are correspondingly arranged on the same horizontal plane, the operation display unit is a touch screen type control panel, and doctors can set a plurality of control parameters through corresponding buttons and can display the parameters detected by the sensors.

Every be fixed with convex slide rail on the both sides lateral wall face of culture room, place on the slide rail and place the dish, place and set up the fixed slot that a plurality of arrays were arranged on the dish, the fixed slot is used for placing the culture dish of cultivateing anaerobe, every the fixed slot next door all is provided with the pilot lamp, the pilot lamp is used for instructing the state of culture dish.

The indicator light has three states, namely a red light state, a yellow light state and an off state, wherein the off state is used for indicating that no culture dish is placed in a certain fixing groove, the yellow light state is used for indicating that a culture dish is placed in the certain fixing groove, and the red light state is used for indicating that the culture dish needs to be added with a reagent or needs to be taken out of the fixing groove. When the culture dish needs to be taken out, a doctor can open the observation window, draw out the placing tray, quickly take out the culture dish needing to be taken out, push in the placing tray and close the observation window.

It should be noted that, after the substrate and the culture solution are added to the culture dish, it generally takes two days for anaerobic bacteria to start to grow, so the culture period of anaerobic bacteria is longer than that of aerobic bacteria, therefore, when a certain culture chamber is opened and closed instantly, the gas composition in the culture chamber will change to some extent, but the change is not so great, and the hypoxic/anoxic environment is not changed.

Further, still be provided with the image acquisition unit in the fixed slot, the image acquisition unit both can be used to the position of detection culture dish, also can be used to gather the colony image data in the culture dish.

The reagent adding unit is correspondingly arranged above the placing plate and consists of a first micro motor, a first threaded rod, a bearing, a cross beam, a reagent conveying pipe, a second micro motor, a second threaded rod, a first sliding joint, a second sliding joint, a reagent outlet pipe, a fixing frame and a reagent adding port. The first/second threaded rods are fixed on the two side wall surfaces of each culture chamber, one end of each first threaded rod is fixed with a first micro motor, the first/second threaded rods are provided with external threads, the first sliding joints are connected through the external threads, a cross beam is fixed between the two first sliding joints, the cross beam is a threaded rod with external threads, a second sliding joint is connected through the external thread, a second micromotor is fixedly connected with the upper part of the second sliding joint, the second micromotor is used for driving the second sliding joint to move on the cross beam, the lower part of the second sliding joint is provided with a reagent outlet pipe, the second sliding joint is internally provided with a corresponding connecting channel, the reagent outlet pipe is connected with the reagent delivery pipe through the connecting channel, the tail end of the reagent conveying pipe is connected with a reagent adding port arranged on the outer side of the incubator.

Through first micromotor and second sliding joint, can adjust the position of reagent outlet pipe in the plane, when the pilot lamp of a certain fixed slot is "red light" state, the position of this pilot lamp is detected and gathered to the image acquisition unit, and then transmits image data to bacteria cultivation device control unit on, and bacteria cultivation device control unit control reagent adds the unit action, reagent addition unit stops after removing the reagent outlet pipe to the top of this fixed slot, through bacteria cultivation device control unit control reagent outlet pipe to this culture dish in the fixed slot adds reagent.

The gas-supply pipe end divide into a plurality of air inlet branch, every be provided with the control valve on the air inlet branch, every air inlet branch is connected with one side gas circuit of cultivateing the room, every cultivate the opposite side of room and be connected with exhaust branch, every be provided with the control valve on the exhaust branch, a plurality of exhaust branch are connected with one side in confluence chamber, the opposite side in confluence chamber through purify the connecting pipe with purify the unit gas circuit and connect.

The invention also comprises a control system of the pollution-prevention anaerobic bacteria culture device for the clinical laboratory, wherein the control system is used for controlling the pollution-prevention anaerobic bacteria culture device for the clinical laboratory and comprises a memory, a processor, a controller, a power supply unit, an operation display unit, an information input end and an information output end, the memory is connected with the processor, the processor is connected with the input end of the controller, the information input end is connected with the memory, the memory is also connected with the display unit, and the information output end is connected with the output end of the controller.

The information input end comprises a CO2 concentration sensor, an oxygen concentration sensor, a temperature sensor, an image acquisition unit and an image information converter connected with the image acquisition unit. CO2 concentration sensor, oxygen concentration sensor, temperature sensor all set up in the cultivateing indoorly, CO2 concentration sensor is used for detecting the CO2 concentration in the cultivateing indoorly, oxygen concentration sensor is used for detecting the oxygen concentration in the cultivateing indoorly, temperature sensor is used for detecting the temperature in the cultivateing indoorly.

The information output end further comprises a PTC heating piece and a PTC refrigerating piece, and the PTC heating piece and the PTC refrigerating piece are connected with the bacteria culture device control unit and used for adjusting the temperature in the culture room.

The clinical laboratory practitioner can set the preset CO2 concentration value, the preset oxygen concentration value and the preset temperature range on the screen of the operation display unit.

The invention also comprises an operation method of the pollution-prevention type anaerobic bacteria culture device based on the clinical laboratory, which comprises the following steps:

SS1 when the CO2 concentration sensor detects that the concentration of CO2 in a certain incubator exceeds the preset value of the concentration of CO2, the controller controls to open the control valve on the exhaust branch pipe of the incubator and simultaneously starts the purification unit to sterilize and purify the exhaust.

SS2 when the oxygen concentration sensor detects that the oxygen concentration of a certain incubator exceeds the preset oxygen concentration value, the controller controls to open the control valves of the air inlet branch pipe and the air outlet branch pipe of the incubator at the same time, and then the controller controls to start the purification unit to sterilize and purify the exhaust gas.

SS3, the preset range of the temperature range set by the scheme is [30 ℃, 39 ℃), and when the temperature detected by the temperature sensor is lower than the preset range of the temperature range, namely the temperature is lower than 30 ℃, the controller controls the PTC heating sheet to heat; when the temperature detected by the temperature sensor is higher than the preset temperature range, namely the temperature is higher than 39 ℃, the controller controls the PTC refrigerating sheet to refrigerate.

SS4, when the image acquisition unit detects that a culture dish is placed in the fixed slot, the controller controls the indicator light to display as a yellow light, when the image acquisition unit does not detect that the culture dish is placed in the fixed slot, the controller controls the indicator light to turn off, when the memory compares the data uploaded by the image acquisition unit with preset colony data, the controller controls the indicator light to display as a red light after determining that a reagent needs to be added to the colony or the colony grows mature, and when the controller displays the red light, the controller drives the reagent adding unit to act, moves the reagent outlet pipe to the position right above the fixed slot where the reagent needs to be added, and controls the reagent adding unit to add the reagent to the culture dish in the fixed slot.

In conclusion, the anti-pollution anaerobic bacteria culture device for the clinical laboratory can effectively solve the problems that the anaerobic conditions for anaerobic bacteria culture cannot be effectively guaranteed and are easily interfered by external factors in the prior art by a method of introducing nitrogen and exhausting air, can prevent anaerobic bacteria from being easily polluted in the growth process by embedding the sealing rubber strips at the edge of the observation window, can better control the growth environment of the anaerobic bacteria by controlling the temperature and the content of metabolite carbon dioxide of the anaerobic bacteria to promote the formation of anaerobic bacterial colonies, and simultaneously reduces the adverse effect of manually and directly operating a culture dish to the maximum extent in the culture process of the anaerobic bacteria.

Drawings

FIG. 1 is a front elevation view of an anti-pollution anaerobic bacteria culture device for clinical laboratory;

FIG. 2 is a side view of an anti-pollution type anaerobic bacteria culture device for clinical laboratory;

FIG. 3 is a top view of a mobile reagent addition unit of the present invention;

FIG. 4 is a front elevation view of a mobile reagent addition unit of the present invention;

FIG. 5 is a schematic view of the gas circuit of an anti-pollution anaerobic bacteria culture device for clinical laboratory;

FIG. 6 is a schematic view of a control system of an anti-pollution anaerobic bacteria culture device for clinical laboratory according to the present invention;

in the figure, 1-incubator; 2-operation display unit, 3-purification unit, 4-roller; 5-placing a fixed platform; 6-nitrogen gas storage tank; 7-gas pipe; 8-push-pull handle; 9-observation window; 10-finger shaped grooves; 11-exhaust orifice; 12-a slide rail; 13-placing the tray; 14-a fixed groove; 15-culture dish; 16-an indicator light; 17-a first micromotor; 18-a first threaded rod; 19-a bearing; 20-a cross beam; 21-reagent conveying pipe; 22-a second micromotor; 23-a second threaded rod; 24-a first slip joint; 25-a second slip joint; 26-a reagent outlet tube; 27-a fixed mount; 28-a control valve; 29-a manifold chamber; 30-purge connection tube.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

Referring to fig. 1-2, the contamination-prevention bacteria culture apparatus for clinical laboratory of the present invention includes an incubator 1, an operation display unit 2, a purification unit 3, a roller 4, a push-pull handle 8, a nitrogen supply unit, a reagent adding unit, and a bacteria culture apparatus control unit. The incubator 1 is of a cuboid structure, the upper part of the incubator 1 is connected with the display unit 2, one side of the incubator 1 is fixedly connected with the operation panel 3, one side of the incubator 1 is fixedly connected with the door-shaped push-pull handle 8, the bottom of the incubator 1 is provided with a plurality of rollers 4, and the incubator 1 can be pushed, pulled or parked easily through the doctor push-pull handle 8 and the rollers 4; one side of incubator 1 is placed through holding fixed station 5 and is supplied the nitrogen unit, supply the nitrogen unit to include nitrogen gas holder 6, gas-supply pipe 7 and be located the control valve on the gas-supply pipe 7, be provided with a plurality of cultivateing rooms in the incubator 1, set up in every cultivates the indoor reagent and add the unit, reagent adds unit, control valve, display element 2, operating panel 3 and all is connected with bacteria culture apparatus control system electricity.

Every cultivates the room and all has the rectangle viewing aperture that forms on incubator 1 panel, and one side of rectangle viewing aperture articulates there is observation window 9, and observation window 9 is the rectangular plate-like structure, adopts double glazing to make, and the edge of observation window 9 is inlayed and is equipped with joint strip (not drawn in the picture) for when observation window 9 lock is on the rectangle viewing aperture, seal up the gap of lock.

The bacteria culture device control unit is arranged at the top of the incubator 1 and electrically connected with an external power supply, the bacteria culture device control unit and the operation display unit 2 are correspondingly installed on the same horizontal plane, the operation display unit 2 is a touch screen type control panel, and doctors can set a plurality of control parameters through corresponding buttons and can display the parameters detected by the sensors.

Referring to fig. 2-4, two side wall surfaces of each culture chamber are fixed with a protruding slide rail 12, a rectangular placing tray 13 is placed on the slide rail 12, a plurality of fixing grooves 14 arranged in a rectangular array are formed in the placing tray 13, the fixing grooves 14 are used for placing culture dishes 15 for culturing anaerobic bacteria, an indicator light 16 is arranged beside each fixing groove 14, and the indicator light 16 is used for indicating the state of the culture dishes 15. When the observation window 9 is opened, the clinical laboratory technician can take out the placing tray 13 from the inside of the incubation chamber or put the placing tray 13 into the inside of the incubation chamber.

The indicator light 16 has three states, namely, a "red light" state, a "yellow light" state and an "off" state, the "off" state is used to indicate that no culture dish is placed in a certain fixing groove 14, the "yellow light" state is used to indicate that a culture dish is placed in a certain fixing groove 14, and the "red light" state is used to indicate that the culture dish needs to be added with a reagent or taken out of the fixing groove. When the culture dish needs to be taken out, a doctor can open the observation window 9, draw out the placing tray 13, quickly take out the culture dish needing to be taken out, push in the placing tray 13 and close the observation window 9.

Further, still be provided with image acquisition unit in fixed slot 14, image acquisition unit both can be used to the position of detection culture dish, also can be used to gather the colony image data in the culture dish.

A reagent adding unit is correspondingly arranged above the placing plate 13 and consists of a first micromotor 17, a first threaded rod 18, a bearing 19, a cross beam 20, a reagent conveying pipe 21, a second micromotor 22, a second threaded rod 23, a first sliding joint 24, a second sliding joint 25, a reagent outlet pipe 26, a fixing frame 27 and a reagent adding port. A first threaded rod 18 and a second threaded rod 23 are fixed on two side wall surfaces of each culture chamber, a first micro motor 17 is fixed at one end of the first threaded rod 18, the first micro motor 17 is used for driving the first threaded rod 18 to rotate, external threads are arranged on the first threaded rod 18 and the second threaded rod 23, the first threaded rod 18 and the second threaded rod 23 are connected with a first sliding joint 24 through external threads, a cross beam 20 is fixed between the two first sliding joints 24, the cross beam 20 is a threaded rod with external threads and is connected with a second sliding joint 25 through the external threads, a second micro motor 22 is fixedly connected with the upper part of the second sliding joint 25, the second micro motor 22 is used for driving the second sliding joint 25 to move on the cross beam 20, a reagent outlet pipe 26 is arranged at the lower part of the second sliding joint 25, a corresponding connecting channel (not shown in the figure) is arranged in the second sliding joint 25, and the reagent outlet pipe 26 is connected with the reagent conveying pipe 21 through the connecting channel, the end of the reagent feed tube 21 is connected to a reagent addition port (not shown) provided outside the incubator 1.

Through first micromotor 17 and second sliding joint 25, can adjust the position of reagent outlet pipe 26 in the plane, when the pilot lamp 16 of a certain fixed slot 14 is "red light" state, the image acquisition unit detects and gathers the position of this pilot lamp 16, and then passes image data up to the bacteria cultivation device control unit, the action of bacteria cultivation device control unit control reagent addition unit, reagent addition unit stops after moving reagent outlet pipe 26 to the top of this fixed slot 14, add reagent to the culture dish 15 in this fixed slot 14 through bacteria cultivation device control unit control reagent outlet pipe 26.

Referring to fig. 5, the gas pipe 7 is divided into a plurality of gas inlet branch pipes, each gas inlet branch pipe is provided with a control valve 28, each gas inlet branch pipe is connected with a gas circuit on one side of the cultivation chamber, the other side of each cultivation chamber is connected with a gas outlet branch pipe, each gas outlet branch pipe is provided with a control valve 28, the plurality of gas outlet branch pipes are connected with one side of the confluence cavity 29, and the other side of the confluence cavity 29 is connected with the gas circuit of the purification unit 3 through a purification connecting pipe 30. When the oxygen content of a certain culture chamber needs to be reduced, the control valve 28 on the air inlet branch pipe connected with the culture chamber is opened, the control valve 28 on the air outlet branch pipe connected with the culture chamber is opened at the same time, and in the process of filling nitrogen, the exhaust gas is sterilized and purified through the purification unit 3 and then is discharged into the chamber, so that the environment pollution caused by the ineffective purification treatment of the exhaust gas can be prevented.

Example 2

Referring to fig. 5, the invention further comprises a control system of the pollution-prevention anaerobic bacteria culture device for clinical laboratory, which is used for controlling the pollution-prevention anaerobic bacteria culture device for clinical laboratory as described in embodiment 1, and the control system comprises a memory, a processor, a controller, a power supply unit, an operation display unit, an information input end and an information output end, wherein the memory is connected with the processor, the processor is connected with the input end of the controller, the information input end is connected with the memory, the memory is further connected with the display unit, and the information output end is connected with the output end of the controller.

The scheme also comprises a PTC heating piece and a PTC refrigerating piece, wherein the PTC heating piece and the PTC refrigerating piece are connected with the bacterial culture device control unit and used for adjusting the temperature in the culture chamber. The number of the PTC heating sheets or PTC refrigerating sheets arranged in each culture chamber is not more than 3.

Claims

1. The utility model provides an anti-pollution formula anaerobe culture apparatus of clinical laboratory, includes incubator, operation display element, purification unit, gyro wheel, push-and-pull handle, supplies nitrogen unit, reagent interpolation unit and bacteria culture apparatus control system, its characterized in that: the upper part of the incubator is connected with the operation display unit, one side of the incubator is fixedly connected with the purification unit, one side of the incubator is fixedly connected with a door-shaped push-pull handle, and the bottom of the incubator is provided with a plurality of rollers; one side of incubator is put the fixed nitrogen unit that supplies of placing of fixed station through holding, supply the nitrogen unit to include nitrogen gas holder, gas-supply pipe and be located the control valve on the gas-supply pipe, be provided with a plurality of rooms of cultivateing, every in the incubator cultivate the room all with purification unit gas circuit intercommunication, every cultivate indoor all being provided with reagent interpolation unit, purification unit, control valve, operation display unit all are connected with bacteria cultivation device control system electricity.

2. The contamination-preventive bacteria culture apparatus for clinical laboratory according to claim 1, wherein: every the culture room all has the rectangle viewing aperture that forms on the incubator panel, one side of rectangle viewing aperture articulates there is the observation window, the observation window adopts transparent double-layer material to make, the edge of observation window inlays and is equipped with joint strip for when the observation window lock is on the rectangle viewing aperture, seal up the lock gap.

3. The contamination-preventive bacteria culture apparatus for clinical laboratory according to any one of claims 1 to 2, wherein: the control unit of the bacterial culture device is arranged at the top of the incubator and electrically connected with an external power supply, the control unit of the bacterial culture device and the operation display unit are correspondingly arranged on the same horizontal plane, and the operation display unit is a touch screen type control panel.

4. A clinical laboratory contamination-preventive bacteria culture apparatus according to any one of claims 1 to 3, characterized in that: every be fixed with convex slide rail on the both sides lateral wall face of culture room, place on the slide rail and place the dish, place and set up the fixed slot that a plurality of arrays were arranged on the dish, the fixed slot is used for placing the culture dish of cultivateing anaerobe, every the fixed slot next door all is provided with the pilot lamp, the pilot lamp is used for instructing the state of culture dish.

5. The contamination-preventive bacteria culture apparatus for clinical laboratory according to any one of claims 1 to 4, wherein: and an image acquisition unit is also arranged in the fixing groove.

6. The contamination-preventive bacteria culture apparatus for clinical laboratory according to any one of claims 1 to 5, wherein: the reagent adding unit is correspondingly arranged above the placing plate and consists of a first micro motor, a first threaded rod, a bearing, a cross beam, a reagent conveying pipe, a second micro motor, a second threaded rod, a first sliding joint, a second sliding joint, a reagent outlet pipe, a fixing frame and a reagent adding port. The first/second threaded rods are fixed on the two side wall surfaces of each culture chamber, one end of each first threaded rod is fixed with a first micro motor, the first/second threaded rods are provided with external threads, the first sliding joints are connected through the external threads, a cross beam is fixed between the two first sliding joints, the cross beam is a threaded rod with external threads, a second sliding joint is connected through the external thread, a second micromotor is fixedly connected with the upper part of the second sliding joint, the second micromotor is used for driving the second sliding joint to move on the cross beam, the lower part of the second sliding joint is provided with a reagent outlet pipe, the second sliding joint is internally provided with a corresponding connecting channel, the reagent outlet pipe is connected with the reagent delivery pipe through the connecting channel, the tail end of the reagent conveying pipe is connected with a reagent adding port arranged on the outer side of the incubator.

7. The contamination-preventive bacteria culture apparatus for clinical laboratory according to any one of claims 1 to 6, wherein: the gas-supply pipe end divide into a plurality of air inlet branch, every be provided with the control valve on the air inlet branch, every air inlet branch is connected with one side gas circuit of cultivateing the room, every cultivate the opposite side of room and be connected with exhaust branch, every be provided with the control valve on the exhaust branch, a plurality of exhaust branch are connected with one side in confluence chamber, the opposite side in confluence chamber through purify the connecting pipe with purify the unit gas circuit and connect.

8. A control system of an anti-pollution type anaerobic bacteria culture device for clinical laboratories, which is used for the anti-pollution type bacteria culture device for clinical laboratories according to any one of claims 1 to 7, and comprises a memory, a processor, a controller, a power supply unit, an operation display unit, an information input end and an information output end, wherein the memory is connected with the processor, the processor is connected with the input end of the controller, the information input end is connected with the memory, the memory is further connected with a display unit, and the information output end is connected with the output end of the controller.

9. The control system of the anti-pollution type anaerobic bacteria culture device for the clinical laboratory according to claim 8, is characterized in that: the information input end comprises a CO2 concentration sensor, an oxygen concentration sensor, a temperature sensor, an image acquisition unit and an image information converter connected with the image acquisition unit. CO2 concentration sensor, oxygen concentration sensor, temperature sensor all set up in the cultivateing indoorly, CO2 concentration sensor is used for detecting the CO2 concentration in the cultivateing indoorly, oxygen concentration sensor is used for detecting the oxygen concentration in the cultivateing indoorly, temperature sensor is used for detecting the temperature in the cultivateing indoorly.

10. An operation method based on a clinical laboratory pollution prevention type anaerobic bacteria culture device and a clinical laboratory pollution prevention type anaerobic bacteria culture device control system are as follows: