CN215593083U

CN215593083U - Carbon dioxide cell culture case with water supply function

Info

Publication number: CN215593083U
Application number: CN202121330091.7U
Authority: CN
Inventors: 刘树民; 乌德春; 文瑜
Original assignee: Shenzhen Kangyuan Jiuyuan Biotechnology Co ltd
Current assignee: Shenzhen Kangyuan Jiuyuan Biotechnology Co ltd
Priority date: 2021-06-15
Filing date: 2021-06-15
Publication date: 2022-01-21
Anticipated expiration: 2031-06-15

Abstract

The utility model discloses a carbon dioxide cell culture box with a water supply function, and relates to the technical field of carbon dioxide cell culture equipment, a glass water tank is arranged in a water supply box for storing water, an ultraviolet lamp bar in a cavity can sterilize and disinfect the interior of the glass water tank, bacterial microorganisms in water and the like can be prevented from entering the interior of a box body to pollute cell culture in a culture dish, and smooth cell culture is ensured; the inside air exchange of box can be realized through the blow vent that sets up in box top both sides, and the blow vent top sets up annular cutting ferrule, and the inside of annular cutting ferrule sets up HEPA high efficiency filter screen, can effectively filter bacterium and dust particle in the outside air, eliminates and prevents outside air and the inside cross contamination of box, makes to be in sterile condition in the incubator.

Description

Carbon dioxide cell culture case with water supply function

Technical Field

The utility model relates to the technical field of carbon dioxide cell culture equipment, in particular to a carbon dioxide cell culture box with a water supply function.

Background

The carbon dioxide cell culture box is widely applied to research and production in the scientific fields of microorganisms, medicine, pharmacy, environmental protection, food, livestock breeding and the like. The method is commonly used in the research fields of cell dynamics research, collection of secretion of mammalian cells, carcinogenic or toxicological effects of various physical and chemical factors, research and production of antigens, production of antibodies by culturing hybridoma cells, in-vitro fertilization (IVF), stem cells, tissue engineering, drug screening and the like.

Most current carbon dioxide cell culture case, it is very inconvenient to humidity, temperature and carbon dioxide's control, need artifical regular examining, the adjustment, it is intelligent not high, seriously influence the growing environment of cell in the incubator, frequent carbon dioxide cell culture case that opens and shuts is very unfavorable to the growth of cell, moreover, when supplying water to the incubator inside, cause bacterial microorganism etc. of aquatic to get into the inside cell culture that causes the pollution to the culture dish in easily, therefore we need provide a carbon dioxide cell culture case with water supply function.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a carbon dioxide cell culture box with a water supply function, which can prevent bacterial microorganisms and the like in water from entering the box body to pollute cell culture in a culture dish, and ensure smooth cell culture so as to solve the problems in the background art.

In order to achieve the purpose, the utility model provides the following technical scheme:

the utility model provides a carbon dioxide cell culture case with water supply function, includes the box, one side of box is provided with the chamber door, the inside of box is provided with the support, be provided with the tray on the support, the equidistance array is provided with the standing groove that is used for placing the culture dish on the tray, the interior bottom of box is provided with carbon dioxide consistency transmitter and humidity transducer respectively, the top of box has set gradually carbon dioxide and has added mechanism and humidification mechanism, the interior top of box is provided with temperature sensor, be provided with heating mechanism on the inner wall of box, the top both sides of box are provided with the blow vent.

Preferably, the support is symmetrically provided with two groups, one side of the support is fixedly welded with a supporting block, and the supporting block is provided with a clamping groove.

Preferably, the bottoms of the two sides of the tray are symmetrically provided with clamping blocks, the tray is clamped and fixed on the supporting block through the clamping blocks, and four groups of the trays are arranged on the support at equal intervals.

Preferably, the carbon dioxide adds the mechanism and includes carbon dioxide generator and carbon dioxide conveyer pipe, top one side fixed mounting of box has carbon dioxide generator, carbon dioxide generator's output fixedly connected with carbon dioxide conveyer pipe, the one end of carbon dioxide conveyer pipe runs through the top of box and extends to the interior top fixedly connected with carbon dioxide export of box.

Preferably, the humidifying mechanism comprises a humidifier and a humidifying pipe, the humidifier is fixedly mounted on the other side of the top of the box body, and the output end of the humidifier is fixedly connected with the humidifying pipe.

Preferably, one end of the humidifying pipe penetrates through the top of the box body and extends to the interior of the box body, the top of the humidifying pipe is fixedly connected with a humidifying sprayer, a supporting rod is arranged at the top of the humidifier, a water supply box is arranged at the upper end of the supporting rod, and a glass water tank is embedded in the water supply box.

Preferably, the end of intaking of humidifier leads to pipe and communicates in the interior bottom of glass water tank, the cavity has been seted up to the both sides inner wall of water supply box, the internally mounted of cavity has ultraviolet lamp strip.

Preferably, the heating mechanism comprises a transparent glass cover and a far infrared heating plate, the transparent glass cover is fixedly mounted on the inner walls of the two sides of the box body, and the far infrared heating plate is mounted inside the transparent glass cover.

Preferably, the top of the vent is clamped with an annular clamping sleeve, a filter screen is arranged in the annular clamping sleeve, and the filter screen is an HEPA high-efficiency filter screen layer.

Preferably, a box door is hinged to one side of the box body through a hinge, a visible window is formed in the box door, and a sealing rubber strip is arranged on the edge of the box door.

Compared with the prior art, the utility model has the beneficial effects that:

1. according to the utility model, the carbon dioxide concentration sensor, the humidity sensor and the temperature sensor are sequentially arranged in the box body, the carbon dioxide adding mechanism and the humidifying mechanism are sequentially arranged at the top of the box body, and the heating mechanism is arranged on the inner wall of the box body, so that the control on the carbon dioxide concentration, the temperature and the humidity in the incubator can be realized, the normal growth of cells is ensured, the culture efficiency is improved, manual regular operation is not required, and the working intensity of workers is reduced;

2. the glass water tank is arranged in the water supply box and used for storing water, and the ultraviolet lamp strip in the cavity can sterilize and disinfect the interior of the glass water tank, so that bacterial microorganisms and the like in water can be prevented from entering the box body to pollute cell culture in a culture dish, and smooth cell culture is guaranteed;

3. the inside air exchange of box can be realized through the blow vent that sets up in box top both sides, and the blow vent top sets up annular cutting ferrule, and the inside of annular cutting ferrule sets up HEPA high efficiency filter screen, can effectively filter bacterium and dust particle in the outside air, eliminates and prevents outside air and the inside cross contamination of box, makes to be in sterile condition in the incubator.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic structural diagram of a fixture block according to the present invention;

FIG. 3 is a schematic view showing the construction of a water service box according to the present invention;

FIG. 4 is a schematic structural view of a heating mechanism according to the present invention;

fig. 5 is a schematic structural view of the tray of the present invention.

In the figure: 1. a box body; 2. a box door; 3. a support; 4. a tray; 5. a placement groove; 6. a carbon dioxide concentration sensor; 7. a humidity sensor; 8. a carbon dioxide addition mechanism; 9. a humidifying mechanism; 10. a temperature sensor; 11. a heating mechanism; 12. a vent; 13. a support block; 14. a card slot; 15. a clamping block; 16. a carbon dioxide outlet; 17. a humidifying spray head; 18. a support bar; 19. a water supply box; 20. a glass water tank; 21. a cavity; 22. an ultraviolet lamp bar; 23. an annular ferrule; 24. a filter screen; 25. sealing rubber strips; 801. a carbon dioxide generator; 802. a carbon dioxide delivery pipe; 901. a humidifier; 902. a humidifying pipe; 111. a transparent glass cover; 112. far infrared heating plate.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-5, the present invention provides a technical solution:

the utility model provides a carbon dioxide cell culture case with water supply function, including box 1, one side of box 1 is provided with chamber door 2, the inside of box 1 is provided with support 3, be provided with tray 4 on the support 3, equidistant array is provided with standing groove 5 that is used for placing the culture dish on tray 4, the interior bottom of box 1 is provided with carbon dioxide concentration sensor 6 and humidity transducer 7 respectively, the top of box 1 has set gradually carbon dioxide and has added mechanism 8 and humidification mechanism 9, the interior top of box 1 is provided with temperature sensor 10, be provided with heating mechanism 11 on the inner wall of box 1, the top both sides of box 1 are provided with blow vent 12.

The support 3 symmetry is provided with two sets ofly, and the fixed welding of one side of support 3 has supporting shoe 13, has seted up draw-in groove 14 on the supporting shoe 13, and the both sides bottom symmetry of tray 4 is provided with fixture block 15, and tray 4 passes through fixture block 15 joint to be fixed on supporting shoe 13, and tray 4 is equidistant four sets of being provided with on support 3.

Carbon dioxide adds mechanism 8 and includes carbon dioxide generator 801 and carbon dioxide conveyer pipe 802, top one side fixed mounting of box 1 has carbon dioxide generator 801, carbon dioxide generator 801's output fixedly connected with carbon dioxide conveyer pipe 802, the one end of carbon dioxide conveyer pipe 802 runs through the top of box 1 and extends to box 1 in top fixedly connected with carbon dioxide export 16, heating mechanism 11 includes transparent glass cover 111 and far infrared heating board 112, fixed mounting has transparent glass cover 111 on the both sides inner wall of box 1, the internally mounted of transparent glass cover 111 has far infrared heating board 112.

Humidification mechanism 9 includes humidifier 901 and humidification pipe 902, top opposite side fixed mounting of box 1 has humidifier 901, humidifier 901's output fixedly connected with humidification pipe 902, humidification pipe 902's one end runs through box 1's top and extends to box 1 internal top fixedly connected with humidification shower nozzle 17, humidifier 901's top is provided with bracing piece 18, bracing piece 18's upper end is provided with water supply box 19, water supply box 19's inside is inlayed and is equipped with glass water tank 20, humidifier 901's the end of intaking leads to pipe and communicates in glass water tank 20's interior bottom, cavity 21 has been seted up to water supply box 19's both sides inner wall, cavity 21's internally mounted has ultraviolet lamp strip 22.

The top joint of blow vent 12 has annular cutting ferrule 23, and the inside of annular cutting ferrule 23 is provided with filter screen 24, and filter screen 24 sets up to HEPA high efficiency filter net layer, and chamber door 2 has been installed through hinge joint in one side of box 1, and visual window has been seted up to chamber door 2, and the edge of chamber door 2 is provided with joint strip 25.

According to the utility model, the carbon dioxide concentration sensor 6, the humidity sensor 7 and the temperature sensor 10 are sequentially arranged in the box body 1, the carbon dioxide adding mechanism 8 and the humidifying mechanism 9 are sequentially arranged at the top of the box body 1, and the heating mechanism 11 is arranged on the inner wall of the box body 1, so that the control of the concentration, the temperature and the humidity of the carbon dioxide in the incubator can be realized, the normal growth of cells is ensured, the culture efficiency is improved, manual regular operation is not needed, and the working intensity of workers is reduced;

the glass water tank 20 is arranged in the water supply box 19 for storing water, and the ultraviolet lamp strip 22 in the cavity 21 can sterilize and disinfect the interior of the glass water tank 20, so that bacterial microorganisms and the like in water are prevented from entering the interior of the box body 1 to pollute cell culture in a culture dish, and smooth cell culture is guaranteed;

can realize the inside air exchange of box 1 through vent 12 that sets up in 1 top both sides of box, vent 12 tops set up annular cutting ferrule 23, and the inside of annular cutting ferrule 23 sets up HEPA high efficiency filter screen, can effectively filter bacterium and dust particle in the outside air, eliminates and prevents outside air and the inside cross contamination of box 1, makes the incubator in locate at sterile condition.

When the cell culture dish is used, a cell culture dish is placed in the placing groove 5 on the tray 4, the clamping block 15 at the bottom of the tray 4 is clamped in the clamping groove 14 on the supporting block 13 to fix the tray 4, the box door 2 is closed, during cell culture, the carbon dioxide concentration sensor 6, the humidity sensor 7 and the temperature sensor 10 can monitor the concentration, humidity and temperature of carbon dioxide in the box body 1 in real time, when the carbon dioxide concentration deviation occurs, the carbon dioxide concentration sensor 6 transmits signals to the carbon dioxide adding mechanism 8 which comprises the carbon dioxide generator 801 and the carbon dioxide conveying pipe 802 and is arranged at the top of the box body 1, the carbon dioxide generator 801 is started to generate carbon dioxide and conveys the carbon dioxide to the inside of the box body 1 through the carbon dioxide conveying pipe 802 so as to improve the concentration of the carbon dioxide in the box body 1; when the humidity in the box body 1 is low, the humidity sensor 7 transmits a signal to the humidifying mechanism 9 which comprises a humidifier 901 and a humidifying pipe 902 and is arranged at the top of the box body 1, the humidifier 901 atomizes water in the water supply box 19 and transmits the atomized water to the interior of the box body 1 through the humidifying pipe 902 so as to improve the humidity in the box body 1, wherein the glass water tank 20 is arranged in the water supply box 19 for storing the water, and the ultraviolet lamp strip 22 in the cavity 21 can sterilize and disinfect the interior of the glass water tank 20, so that bacterial microorganisms and the like in the water are prevented from entering the interior of the box body 1 to pollute the cell culture in a culture dish, and the smooth proceeding of the cell culture is ensured; when the temperature in the box body 1 is low, the temperature sensor 10 transmits a signal to the heating mechanism 11 which comprises a transparent glass cover 111 and a far infrared heating plate 112 and is arranged on the inner wall of the box body 1, and the far infrared heating plate 112 works to keep the temperature in the box body 1; the air vents 12 arranged on the two sides of the top of the box body 1 can realize air exchange in the box body 1, the annular clamping sleeve 23 is arranged on the top of the air vent 12, and the HEPA high-efficiency filter screen is arranged in the annular clamping sleeve 23, so that bacteria and dust particles in the outside air can be effectively filtered, cross contamination between the outside air and the inner chamber of the incubator is eliminated and prevented, and the inside of the incubator is in an aseptic state; this device can realize controlling carbon dioxide concentration, temperature and the humidity inside the incubator, has guaranteed the normal growth of cell, has improved cultivation efficiency, need not artifical and regularly operates, has reduced staff's working strength.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a carbon dioxide cell culture case with water supply function, includes box (1), its characterized in that: one side of box (1) is provided with chamber door (2), the inside of box (1) is provided with support (3), be provided with tray (4) on support (3), equidistant array is provided with standing groove (5) that are used for placing the culture dish on tray (4), the interior bottom of box (1) is provided with carbon dioxide concentration sensor (6) and humidity transducer (7) respectively, the top of box (1) has set gradually carbon dioxide and has added mechanism (8) and humidification mechanism (9), the interior top of box (1) is provided with temperature sensor (10), be provided with heating mechanism (11) on the inner wall of box (1), the top both sides of box (1) are provided with blow vent (12).

2. The carbon dioxide cell incubator with the function of supplying water according to claim 1, characterized in that: the support is characterized in that the support (3) is symmetrically provided with two groups, a supporting block (13) is fixedly welded on one side of the support (3), and a clamping groove (14) is formed in the supporting block (13).

3. The carbon dioxide cell incubator with the function of supplying water according to claim 2, wherein: the tray is characterized in that clamping blocks (15) are symmetrically arranged at the bottoms of two sides of the tray (4), the tray (4) is clamped and fixed on the supporting block (13) through the clamping blocks (15), and four groups of the tray (4) are equidistantly arranged on the support (3).

4. The carbon dioxide cell incubator with the function of supplying water according to claim 1, characterized in that: carbon dioxide adds mechanism (8) and includes carbon dioxide generator (801) and carbon dioxide conveyer pipe (802), top one side fixed mounting of box (1) has carbon dioxide generator (801), the output fixedly connected with carbon dioxide conveyer pipe (802) of carbon dioxide generator (801), the one end of carbon dioxide conveyer pipe (802) runs through the top of box (1) and extends to top fixedly connected with carbon dioxide export (16) in box (1).

5. The carbon dioxide cell incubator with the function of supplying water according to claim 1, characterized in that: the humidifying mechanism (9) comprises a humidifier (901) and a humidifying pipe (902), the humidifier (901) is fixedly installed on the other side of the top of the box body (1), and the output end of the humidifier (901) is fixedly connected with the humidifying pipe (902).

6. The carbon dioxide cell incubator with water supply function according to claim 5, wherein: one end of the humidifying pipe (902) penetrates through the top of the box body (1) and extends to the inner top of the box body (1) to be fixedly connected with a humidifying sprayer (17), a supporting rod (18) is arranged at the top of the humidifier (901), a water supply box (19) is arranged at the upper end of the supporting rod (18), and a glass water tank (20) is embedded in the water supply box (19).

7. The carbon dioxide cell incubator with water supply function according to claim 6, wherein: the water inlet end of the humidifier (901) is communicated with the inner bottom of the glass water tank (20) through a water pipe, cavities (21) are formed in the inner walls of the two sides of the water supply box (19), and ultraviolet lamp bars (22) are mounted inside the cavities (21).

8. The carbon dioxide cell incubator with the function of supplying water according to claim 1, characterized in that: heating mechanism (11) include transparent glass cover (111) and far infrared heating board (112), fixed mounting has transparent glass cover (111) on the both sides inner wall of box (1), the internally mounted of transparent glass cover (111) has far infrared heating board (112).

9. The carbon dioxide cell incubator with the function of supplying water according to claim 1, characterized in that: the top of the air vent (12) is clamped with an annular clamping sleeve (23), a filter screen (24) is arranged inside the annular clamping sleeve (23), and the filter screen (24) is an HEPA high-efficiency filter screen layer.

10. The carbon dioxide cell incubator with the function of supplying water according to claim 1, characterized in that: one side of the box body (1) is hinged with a box door (2) through a hinge, a visible window is formed in the box door (2), and a sealing rubber strip (25) is arranged at the edge of the box door (2).