CN108504543A - A kind of stem cell extractor that extraction effect is good - Google Patents
A kind of stem cell extractor that extraction effect is good Download PDFInfo
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- CN108504543A CN108504543A CN201810558749.6A CN201810558749A CN108504543A CN 108504543 A CN108504543 A CN 108504543A CN 201810558749 A CN201810558749 A CN 201810558749A CN 108504543 A CN108504543 A CN 108504543A
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- hose
- shell
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 69
- 238000000605 extraction Methods 0.000 title claims abstract description 47
- 230000000694 effects Effects 0.000 title claims abstract description 44
- 210000003954 umbilical cord Anatomy 0.000 claims abstract description 33
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims abstract description 18
- 210000004700 fetal blood Anatomy 0.000 claims description 30
- 210000002381 plasma Anatomy 0.000 claims description 26
- 210000003743 erythrocyte Anatomy 0.000 claims description 20
- 229920001971 elastomer Polymers 0.000 claims description 15
- 239000005060 rubber Substances 0.000 claims description 15
- 210000004369 blood Anatomy 0.000 claims description 12
- 239000008280 blood Substances 0.000 claims description 12
- 238000001914 filtration Methods 0.000 claims description 5
- 238000005119 centrifugation Methods 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 239000012780 transparent material Substances 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 3
- 239000002002 slurry Substances 0.000 claims description 3
- 229920001612 Hydroxyethyl starch Polymers 0.000 claims description 2
- 238000010521 absorption reaction Methods 0.000 claims description 2
- 239000000284 extract Substances 0.000 claims description 2
- 229940050526 hydroxyethylstarch Drugs 0.000 claims description 2
- 239000012535 impurity Substances 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000004804 winding Methods 0.000 description 2
- 208000019838 Blood disease Diseases 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 201000009030 Carcinoma Diseases 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 206010025323 Lymphomas Diseases 0.000 description 1
- 208000001738 Nervous System Trauma Diseases 0.000 description 1
- 206010029260 Neuroblastoma Diseases 0.000 description 1
- 206010041067 Small cell lung cancer Diseases 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 208000016361 genetic disease Diseases 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 208000014951 hematologic disease Diseases 0.000 description 1
- 238000011134 hematopoietic stem cell transplantation Methods 0.000 description 1
- 208000018706 hematopoietic system disease Diseases 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
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- 238000002360 preparation method Methods 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 208000000587 small cell lung carcinoma Diseases 0.000 description 1
- 238000011476 stem cell transplantation Methods 0.000 description 1
- 210000001550 testis Anatomy 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/05—Means for pre-treatment of biological substances by centrifugation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
Abstract
The invention discloses a kind of good stem cell extractors of extraction effect, including pedestal, the upper surface of the pedestal is fixedly connected with the bottom end of two fixed links respectively, and the opposite face of two fixed links is fixedly connected with the left and right sides of driving device respectively, the inner wall of the driving device is fixedly connected with shaft, the outer wall of the shaft is socketed with bearing, the bearing is connected to the upper surface of pedestal, the top of the shaft is fixedly connected with the lower surface of movable plate, the upper surface of the movable plate is fixedly connected with the lower surface of cabinet exterior, the cabinet wall is provided with filter screen.The good stem cell extractor of the extraction effect, pass through the collective effect of motor, driving wheel, belt, driven wheel, belt, shaft, movable plate and babinet, to realize the extraction of umbilical cord stem cells, and it is easy to operate, so that the DNA purity higher of umbilical cord stem cells, to ensure the using effect of umbilical cord stem cells.
Description
Technical field
The present invention relates to stem cells technology field, the good stem cell extractor of specially a kind of extraction effect.
Background technology
With deepening continuously for stem-cell research and stem cell clinical application, hematopoietic stem cell transplantation has become radical cure and dislikes
Property blood disease (leukaemia, malignant lymphoma, Huppert's disease), alpastic anemia, certain genetic diseases etc. have
Effect means.Stem cell transplantation in recent years gradually be applied to middle and advanced stage malignant solid tumor (Small Cell Lung Cancer, breast cancer, oophoroma,
Carcinoma of testis, neuroblastoma etc.), heart disease, nervous system injury, histoorgan reparation, diabetes, vascular diseases etc. control
It treats.In addition stem cell also has the tissue for enhancing human immunity, repairing lesion or aging, increases man's lifespan, changes mankind's life
The potential such as state are deposited, there is immeasurable medical value.
Umbilical cord stem cells needs extract in Cord blood, and the extraction element of present umbilical cord stem cells is complicated, and not
It is easy to operation, inferior separating effect so that there are a certain amount of blood plasma and red blood cells for the navel stem cell separated, to reduce
The using effect of umbilical cord stem cells.
Invention content
(1) the technical issues of solving
In view of the deficiencies of the prior art, it the present invention provides a kind of good stem cell extractor of extraction effect, solves existing
Umbilical cord stem cells extraction element it is complicated, and be not easy to operate, inferior separating effect so that the navel separated is dry thin
The problem of there are a certain amount of blood plasma and red blood cells by born of the same parents, using effect to reduce umbilical cord stem cells.
(2) technical solution
To achieve the above object, the present invention provides the following technical solutions：A kind of stem cell extractor that extraction effect is good, packet
Pedestal is included, the upper surface of the pedestal is fixedly connected with the bottom end of two fixed links respectively, and the opposite face of two fixed links point
It is not fixedly connected with the left and right sides of driving device, the inner wall of the driving device is fixedly connected with shaft, the shaft
Outer wall is socketed with bearing, and the bearing is connected to the upper surface of pedestal.
The top of the shaft is fixedly connected with the lower surface of movable plate, the upper surface of the movable plate and cabinet exterior
Lower surface is fixedly connected, and the cabinet wall is provided with filter screen, the left and right sides of the filter screen respectively in babinet
The left and right sides of wall are fixedly connected, and the upper surface of the babinet is provided with funnel, the bottom end of the funnel and the upper table of babinet
Face is connected, and the upper surface of the funnel and the lower surface of cover board overlap, and the upper surface of the cover board passes through hinge and fixed plate
Upper surface it is hinged, the lower surface of the fixed plate is fixedly connected with the upper surface of cabinet exterior.
The left side of the babinet is connected with sliding sleeve, and the inner wall of the sliding sleeve is socketed with hose, and the right end of the hose prolongs
The inside of babinet is extended to, and the left end of hose is connected with the right side of first shell, and the outer wall of hose is connected to fixed block
In the groove that front opens up, the left side of the fixed block is fixedly connected with the right end of connecting rod, the bottom end of the connecting rod with
The upper surface of first shell outer wall is fixedly connected, and the lower surface of the first shell is fixedly connected with the upper surface of movable plate, and
The outer wall of the inner wall of first shell and the first rubber pad overlaps, and the inner wall of first rubber pad is connected with the first push plate, and the
The left side of one push plate is fixedly connected with the right end of first support bar, the right side of the left end of the first support bar and the first push rod
Face is fixedly connected.
The upper surface of the movable plate is fixedly connected with the lower surface of second shell outer wall, the left side of the second shell
It is connected with the right end of discharge nozzle, the left end of the discharge nozzle extends to the bottom of cabinet wall, the inner wall of the second shell
It is overlapped with the outer wall of the second rubber pad, the inner wall of second rubber pad is connected with the second push plate, and the right side of the second push plate
It is fixedly connected with the left end of second support bar, the right end of the second support bar is fixedly connected with the left side of the second push rod.
Preferably, the driving device includes motor, the left and right sides of the motor body and the left and right two of fixed link
Side is fixedly connected, and the output shaft of motor is fixedly connected with the lower surface of driving wheel, the driving wheel by belt with it is driven
Wheel is sequentially connected, and the inner wall of the driven wheel is fixedly connected with shaft.
Preferably, the babinet is transparent material, and the first shell and second shell are transparent glass material.
Preferably, the shape of the cover board is rectangle, and the length and width of cover board is all higher than the diameter of funnel.
Preferably, the shape of the connecting rod is L-shaped, and the shape of the movable plate is rectangle.
Preferably, the upper surface of the pedestal is provided with power supply, and the upper surface of the power supply is provided with switch.
Preferably, the output end of the power supply is electrically connected with the input terminal of switch, the output end of the switch and motor
Input terminal is electrically connected, the model YVF7124 frequency control motors of the motor.
(3) advantageous effect
The present invention provides a kind of good stem cell extractors of extraction effect, have following advantageous effect：
(1), the good stem cell extractor of the extraction effect, by motor, driving wheel, belt, driven wheel, belt, shaft,
The output shaft band driving wheel of the collective effect of movable plate and babinet, motor rotates, and driving wheel leads to belt and rotated with driven wheel, from
And so that shaft is rotated with movable plate, so that body pivot so that Cord blood is divided under the action of rotating centrifugation
Layer to realize the extraction of umbilical cord stem cells, and operates so that umbilical cord stem cells are detached with blood plasma and red blood cell respectively
It is convenient, so that the DNA purity higher of umbilical cord stem cells, to ensure the using effect of umbilical cord stem cells.
(2), the good stem cell extractor of the extraction effect pulls the first push rod to the left by the way that the first push rod is arranged so that
First support bar is moved downward with the first push plate so that blood plasma enters hose under the action of hose adsorbs, so that blood
Slurry enters first shell from hose, to realize the extraction for blood plasma so that blood plasma and umbilical cord stem cells separation, to protect
Hinder the purity of navel blood stem cell extraction, and accelerates the rate of filtration of Cord blood.
(3), the good stem cell extractor of the extraction effect pulls to the right the second push rod by the way that the second push rod is arranged so that
Second support bar is moved right with the second push plate so that the red blood cell of lower layer is under the action of discharge nozzle adsorbs, from discharge nozzle
Into second shell, to realize the extraction for red blood cell so that red blood cell and umbilical cord stem cells separation, to ensure
The purity of navel blood stem cell extraction.
(4), the good stem cell extractor of the extraction effect, by be arranged filter screen so that Cord blood from funnel into
Row babinet, Cord blood filter out the impurity such as the clot condensed in Cord blood under the action of filter screen.
(5), the good stem cell extractor of the extraction effect, by the way that fixed block is arranged so that hose is connected on fixed block
Groove in, to realize the fixation of hose, it is therefore prevented that hose falling during movable plate rotates and wind, and this
Inventive structure is compact, reasonable design, highly practical.
Description of the drawings
Fig. 1 is the cross-sectional view that the present invention faces；
Fig. 2 is the structural schematic diagram that the present invention faces.
In figure：1 pedestal, 2 fixed links, 3 driving devices, 31 motors, 32 driving wheels, 33 belts, 34 driven wheels, 4 shafts, 5
Bearing, 6 movable plates, 7 babinets, 8 filter screens, 9 funnels, 10 cover boards, 11 hinges, 12 fixed plates, 13 sliding sleeves, 14 hoses, 15 grooves,
16 fixed blocks, 17 connecting rods, 18 first shells, 19 first push plates, 20 first support bars, 21 first push rods, 22 second shells, 23
Discharge nozzle, 24 second push plates, 25 second support bars, 26 second push rods, 27 power supplys, 28 switches, 29 first rubber pads, 30 second rubbers
Rubber cushion.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
As shown in Figs. 1-2, the present invention provides a kind of technical solution：A kind of stem cell extractor that extraction effect is good, including
The upper surface of pedestal 1, pedestal 1 is provided with power supply 27, and the upper surface of power supply 27 is provided with switch 28, by the way that switch 28 is arranged, makes
It is more convenient for the manipulation of 31 working condition of motor to obtain doctor, and the model LW5D-16 universal change-over switches of switch 28, electricity
The output end in source 27 is electrically connected with the input terminal of switch 28, and the output end of switch 28 is electrically connected with the input terminal of motor 31, motor
31 model YVF7124 frequency control motors are frequency control motor by the way that motor 31 is arranged, are exported hence for motor 31
The rotating speed of axis is controlled, more preferable to the centrifugal effect of Cord blood, so that the refining effect of umbilical cord stem cells is more preferable, bottom
The upper surface of seat 1 is fixedly connected with the bottom end of two fixed links 2 respectively, by the way that fixed link 2 is arranged so that the fuselage of motor 31 obtains
Fixation is arrived, it is therefore prevented that the rotation of 31 fuselage of the second motor, the opposite faces of two driving devices 3 left side with driving device 3 respectively
Right two sides are fixedly connected, and the inner wall of driving device 3 is fixedly connected with shaft 4, and driving device 3 includes motor 31,31 machine of motor
The left and right sides of body are fixedly connected with the left and right sides of fixed link 2, by the way that motor 31, the output shaft band master of motor 31 is arranged
Driving wheel 32 rotates, and driving wheel 32 leads to belt 33 and rotated with driven wheel 34, so that shaft 4 is rotated with movable plate 6, to
So that babinet 7 rotate so that Cord blood rotate centrifuge under the action of be layered so that umbilical cord stem cells respectively with blood
Slurry is detached with red blood cell, to realize the extraction of umbilical cord stem cells, and it is easy to operate, so that the extraction of umbilical cord stem cells
Purity higher, to ensure the using effect of umbilical cord stem cells, and the output shaft of motor 31 and the lower surface of driving wheel 32 are solid
Fixed connection, driving wheel 32 are sequentially connected by belt 33 and driven wheel 34, by the way that belt 33 is arranged, to realize driving wheel 32
It is fixedly connected with shaft 4 with the inner wall of the drive connection of driven wheel 34, driven wheel 34, the outer wall of shaft 4 is socketed with bearing 5, axis
Hold 5 upper surfaces for being connected to pedestal 1.
The top of shaft 4 is fixedly connected with the lower surface of movable plate 6, and the shape of movable plate 6 is rectangle, movable plate 6 it is upper
Surface is fixedly connected with the lower surface of 7 outer wall of babinet, and babinet 7 is transparent material, and the material by the way that babinet 7 is arranged is transparent material
Matter, the intake after layering and layering to facilitate doctor's observation Cord blood, 7 inner wall of babinet is provided with filter screen 8, by setting
Filter screen 8 is set, so that Cord blood carries out babinet 7 from funnel 9, Cord blood filters out Cord blood under the action of filter screen 8
The left and right sides of the impurity such as the clot of middle condensation, filter screen 8 are fixedly connected with the left and right sides of 7 inner wall of babinet respectively, case
The upper surface of body 7 is provided with funnel 9, and the bottom end of funnel 9 is connected with the upper surface of babinet 7, upper surface and the cover board 10 of funnel 9
Lower surface overlap joint, the shape of cover board 10 is rectangle, and the length and width of cover board 10 is all higher than the diameter of funnel 9, by setting
Cover board 10 is set, so as to avoid pollution of the impurity such as process dust extracted in umbilical cord stem cells for Cord blood, cover board 10
Upper surface is hinged by the upper surface of hinge 11 and fixed plate 12, and the lower surface of fixed plate 12 is solid with the upper surface of 7 outer wall of babinet
Fixed connection.
The left side of babinet 7 is connected with sliding sleeve 13, by the way that sliding sleeve 13 is arranged, reduces rubbing between sliding sleeve 13 and hose 14
It wipes, to ensure that the service life of hose 14, the inner wall of sliding sleeve 13 are socketed with hose 14, by the way that hose 14 is arranged, pushes soft
Pipe 14 is so that the right end of hose 14 extends toward 7 inner wall of babinet lower section so that the bottom end of hose 14 is contacted with plasma layer, to realize
Intake for blood plasma, the right end of hose 14 extend to the inside of babinet 7, and the right side of the left end of hose 14 and first shell 18
Side is connected, and the outer wall of hose 14 is connected in the groove 15 that 16 front of fixed block opens up, and by the way that fixed block 16 is arranged, makes
It obtains hose 14 to be connected in the groove 15 on fixed block 16, to realize the fixation of hose 14, it is therefore prevented that hose 14 is in activity
Falling and winding during the rotation of plate 6, the left side of fixed block 16 is fixedly connected with the right end of connecting rod 17, connecting rod 17
Bottom end be fixedly connected with the upper surface of 18 outer wall of first shell, the shape of connecting rod 17 is L-shaped, the lower surface of first shell 18
It is fixedly connected with the upper surface of movable plate 6, first shell 18 and second shell 22 are transparent glass material, and first shell 18
Inner wall and the outer wall of the first rubber pad 29 overlap, the inner wall of the first rubber pad 29 is connected with the first push plate 19, and the first push plate
19 left side is fixedly connected with the right end of first support bar 20, the right side of the left end of first support bar 20 and the first push rod 21
It is fixedly connected, by the way that the first push rod 21 is arranged, pulls the first push rod 21 to the left so that first support bar 20 is with the first push plate 19
It moves downward so that blood plasma enters hose 14 under the action of hose 14 adsorbs, so that blood plasma enters first from hose 14
Shell 18, to realize the extraction for blood plasma so that blood plasma and umbilical cord stem cells separation, to ensure navel blood stem cell
The purity of extraction, and accelerate the rate of filtration of Cord blood.
The upper surface of movable plate 6 is fixedly connected with the lower surface of 22 outer wall of second shell, the left side of second shell 22 with
The right end of discharge nozzle 23 is connected, and the left end of discharge nozzle 23 extends to the bottom of 7 inner wall of babinet, the inner wall of second shell 22 and
The outer wall 30 of two rubber pads overlaps, and the inner wall of the second rubber pad 30 is connected with the second push plate 24, and the right side of the second push plate 24
It being fixedly connected with the left end of second support bar 25, the right end of second support bar 25 is fixedly connected with the left side of the second push rod 26,
By the way that the second push rod 26 is arranged, the second push rod 26 is pulled to the right so that second support bar 25 is moved right with the second push plate 24,
So that the red blood cell of lower layer is under the action of discharge nozzle 23 adsorb, from the entrance second shell 22 of discharge nozzle 23, to realize pair
In the extraction of red blood cell so that red blood cell and umbilical cord stem cells separation, to ensure the purity of navel blood stem cell extraction.
In use, doctor is by pulling cover board 10 so that cover board 10 and funnel 9 detach, and then pour into umbilical cord into babinet 7
Hydroxyethyl starch solution is added in babinet 7, then covers cover board 10 again by blood, doctor so that Cord blood carries out case by strainer 8
It inside body 7, while turning left and pulling the first push rod 21, to accelerate the rate of filtration of Cord blood, when Cord blood is completely into case
When 7 inside of body, then by Operation switch 28 so that motor 31 works, the output shaft band driving wheel 32 of motor 31 rotates doctor,
Driving wheel 32 leads to belt 33 and is rotated with driven wheel 34, so that shaft 4 is rotated with movable plate 6, so that 7 turns of babinet
It is dynamic so that Cord blood is layered rotating the under the action of of centrifuging, after Cord blood centrifuges, the usual Operation switch of doctor 28
31 stop motion of motor, Cord blood is made to be separated into three layers, upper layer is blood plasma, and middle level is umbilical cord stem cells, and lower layer is red blood cell,
Then doctor is by pulling to the right the second push rod 26 so that second support bar 25 is moved right with the second push plate 24 so that under
The red blood cell of layer enters second shell 22 under the action of discharge nozzle 23 adsorbs, from discharge nozzle 23, after the completion of red blood cell absorption,
Doctor pulls hose 14 so that hose 14 and groove 15 detach, and push hose 14 so that the right end of hose 14 is toward under 7 inner wall of babinet
Fang Yanshen so that the bottom end of hose 14 is contacted with plasma layer, and then doctor by pulling the first push rod 21 to the left so that first
Strut 20 is moved downward with the first push plate 19 so that and blood plasma enters hose 14 under the action of hose 14 adsorbs, so that
Blood plasma enters first shell 18 from hose 14, after the completion of blood plasma extraction, the groove 15 on making hose 14 and fixed block 16
Clamping, then doctor by Operation switch 28 so that motor 31 works so that the output shaft of motor 31 with higher rotational speed,
Above step is repeated, so that storing the higher umbilical cord stem cells of purity in babinet 7.
It can to sum up obtain, 1, the good stem cell extractor of the extraction effect, by motor 31, driving wheel 32, belt 33, driven
The output shaft band driving wheel 32 of the collective effect of wheel 34, belt 33, shaft 4, movable plate 6 and babinet 7, motor 31 rotates, actively
32 logical belts 33 are taken turns to rotate with driven wheel 34, so that shaft 4 is rotated with movable plate 6, so that babinet 7 rotates,
So that Cord blood is layered under the action of rotating centrifugation, so that umbilical cord stem cells divide with blood plasma and red blood cell respectively
From, to realize the extraction of umbilical cord stem cells, and it is easy to operate, so that the DNA purity higher of umbilical cord stem cells, from
And the using effect of umbilical cord stem cells is ensured.
2, the good stem cell extractor of the extraction effect pulls the first push rod 21, makes to the left by the way that the first push rod 21 is arranged
First support bar 20 is obtained to move downward with the first push plate 19 so that blood plasma enters hose 14 under the action of hose 14 adsorbs,
So that blood plasma enters first shell 18 from hose 14, to realize the extraction for blood plasma so that blood plasma and umbilical cord are dry
Cell detaches, and to ensure the purity of navel blood stem cell extraction, and accelerates the rate of filtration of Cord blood.
3, the good stem cell extractor of the extraction effect pulls to the right the second push rod 26, makes by the way that the second push rod 26 is arranged
Second support bar 25 moved right with the second push plate 24 so that the red blood cell of lower layer under the action of discharge nozzle 23 adsorbs,
Enter second shell 22 from discharge nozzle 23, to realize the extraction for red blood cell so that red blood cell and umbilical cord stem cells point
From to ensure the purity of navel blood stem cell extraction.
4, the good stem cell extractor of the extraction effect, by be arranged filter screen 8 so that Cord blood from funnel 9 into
Row babinet 7, Cord blood filter out the impurity such as the clot condensed in Cord blood under the action of filter screen 8.
5, the good stem cell extractor of the extraction effect, by the way that fixed block 16 is arranged so that hose 14 is connected to fixed block
In groove 15 on 16, to realize the fixation of hose 14, it is therefore prevented that the falling during movable plate 6 rotates of hose 14
And winding, and structure of the invention is compact, reasonable design, it is highly practical.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of variations, modification, replace
And modification, the scope of the present invention is defined by the appended.
Claims (8)
1. a kind of good stem cell extractor of extraction effect, including pedestal (1), it is characterised in that：The upper surface of the pedestal (1)
Be fixedly connected respectively with the bottom end of two fixed links (2), and the opposite face of two fixed links (2) respectively with driving device (3)
Left and right sides are fixedly connected, and the inner wall of the driving device (3) is fixedly connected with shaft (4), the outer wall set of the shaft (4)
It is connected to bearing (5), the bearing (5) is connected to the upper surface of pedestal (1)；
The top of the shaft (4) is fixedly connected with the lower surface of movable plate (6), the upper surface of the movable plate (6) and babinet
(7) lower surface of outer wall is fixedly connected, and babinet (7) inner wall is provided with filter screen (8), the left and right of the filter screen (8)
Two sides are fixedly connected with the left and right sides of babinet (7) inner wall respectively, and the upper surface of the babinet (7) is provided with funnel (9),
The bottom end of the funnel (9) is connected with the upper surface of babinet (7), the following table of the upper surface and cover board (10) of the funnel (9)
Face overlaps, and the upper surface of the cover board (10) is hinged by the upper surface of hinge (11) and fixed plate (12), the fixed plate
(12) lower surface is fixedly connected with the upper surface of babinet (7) outer wall；
The left side of the babinet (7) is connected with sliding sleeve (13), and the inner wall of the sliding sleeve (13) is socketed with hose (14), described soft
The right end of pipe (14) extends to the inside of babinet (7), and the left end of hose (14) is connected with the right side of first shell (18),
And the outer wall of hose (14) is connected in the groove (15) that opens up of fixed block (16) front, the left side of the fixed block (16) with
The right end of connecting rod (17) is fixedly connected, and the bottom end of the connecting rod (17) is fixed with the upper surface of first shell (18) outer wall to be connected
Connect, the lower surface of the first shell (18) is fixedly connected with the upper surface of movable plate (6), and the inner wall of first shell (18) with
The outer wall of first rubber pad (29) overlaps, and the inner wall of first rubber pad (29) is connected with the first push plate (19), and first pushes away
The left side of plate (19) is fixedly connected with the right end of first support bar (20), and the left end of the first support bar (20) is pushed away with first
The right side of bar (21) is fixedly connected；
The upper surface of the movable plate (6) is fixedly connected with the lower surface of second shell (22) outer wall, the second shell (22)
Left side be connected with the right end of discharge nozzle (23), the left end of the discharge nozzle (23) extends to the bottom of babinet (7) inner wall,
The inner wall of the second shell (22) and the outer wall (30) of the second rubber pad overlap, the inner wall clamping of second rubber pad (30)
There is the second push plate (24), and the right side of the second push plate (24) is fixedly connected with the left end of second support bar (25), described second
The right end of supporting rod (25) is fixedly connected with the left side of the second push rod (26).
2. a kind of good stem cell extractor of extraction effect according to claim 1, it is characterised in that：The driving device
(3) include motor (31), the left and right sides of motor (31) fuselage are fixedly connected with the left and right sides of fixed link (2),
And the output shaft of motor (31) is fixedly connected with the lower surface of driving wheel (32), the driving wheel (32) by belt (33) with from
Driving wheel (34) is sequentially connected, and the inner wall of the driven wheel (34) is fixedly connected with shaft (4).
3. a kind of good stem cell extractor of extraction effect according to claim 1, it is characterised in that：The babinet (7)
For transparent material, the first shell (18) and second shell (22) are transparent glass material.
4. a kind of good stem cell extractor of extraction effect according to claim 1, it is characterised in that：The cover board (10)
Shape be rectangle, and the length and width of cover board (10) is all higher than the diameter of funnel (9).
5. a kind of good stem cell extractor of extraction effect according to claim 1, it is characterised in that：The connecting rod
(17) shape is L-shaped, and the shape of the movable plate (6) is rectangle.
6. a kind of good stem cell extractor of extraction effect according to claim 1-5, it is characterised in that：Pedestal (1)
Upper surface is provided with power supply (27), and the upper surface of the power supply (27) is provided with switch (28).
7. a kind of good stem cell extractor of extraction effect according to claim 1-6, it is characterised in that：Power supply (27)
Output end is electrically connected with the input terminal of switch (28), and the output end of the switch (28) is electrically connected with the input terminal of motor (31),
The model YVF7124 frequency control motors of the motor (31).
8. a kind of good stem cell extracting method of extraction effect, it is characterised in that：Doctor is by pulling cover board 10 so that cover board 10
It is detached with funnel 9, Cord blood is then poured into babinet 7, hydroxyethyl starch solution is added in babinet 7, then covers again by doctor
Upper cover plate 10 so that Cord blood is carried out by strainer 8 inside babinet 7, while being turned left and being pulled the first push rod 21, to accelerate navel
The rate of filtration with blood, when Cord blood is completely into 7 inside of babinet, then doctor makes 31 work of motor by Operation switch 28
Make, the output shaft band driving wheel 32 of motor 31 rotates, and driving wheel 32 leads to belt 33 and rotated with driven wheel 34, so that shaft
4 rotate with movable plate 6, so that babinet 7 rotates so that Cord blood is layered under the action of rotating centrifugation, works as navel
After band blood centrifugation, for the usual Operation switch 28 of doctor so that 31 stop motion of motor, Cord blood are separated into three layers, upper layer is blood
Slurry, middle level is umbilical cord stem cells, and lower layer is red blood cell, and then doctor is by pulling to the right the second push rod 26 so that the second support
Bar 25 is moved right with the second push plate 24 so that the red blood cell of lower layer is under the action of discharge nozzle 23 adsorbs, from discharge nozzle 23
Into second shell 22, after the completion of red blood cell absorption, doctor pulls hose 14 so that hose 14 and groove 15 detach, and push
Hose 14 is so that the right end of hose 14 extends toward 7 inner wall of babinet lower section so that the bottom end of hose 14 is contacted with plasma layer, is then cured
Raw pass through pulls the first push rod 21 to the left so that first support bar 20 is moved downward with the first push plate 19 so that blood plasma is soft
Pipe 14 enters hose 14 under the action of adsorbing, so that blood plasma enters first shell 18 from hose 14, is completed when blood plasma extracts
Afterwards, the groove 15 on making hose 14 and fixed block 16 is clamped, and then doctor makes motor 31 work by Operation switch 28,
So that the output shaft of motor 31 repeats above step with higher rotational speed, so that storage purity is higher in babinet 7
Umbilical cord stem cells.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810558749.6A CN108504543A (en) | 2018-06-01 | 2018-06-01 | A kind of stem cell extractor that extraction effect is good |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810558749.6A CN108504543A (en) | 2018-06-01 | 2018-06-01 | A kind of stem cell extractor that extraction effect is good |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108504543A true CN108504543A (en) | 2018-09-07 |
Family
ID=63402196
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810558749.6A Pending CN108504543A (en) | 2018-06-01 | 2018-06-01 | A kind of stem cell extractor that extraction effect is good |
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| Country | Link |
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| CN (1) | CN108504543A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111808720A (en) * | 2020-07-27 | 2020-10-23 | 湖北明德健康科技有限公司 | Stem cell separation device |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203395382U (en) * | 2013-06-24 | 2014-01-15 | 江阴大田科技服务有限公司 | Hose guide rack |
| CN104056728A (en) * | 2014-07-07 | 2014-09-24 | 古廷伟 | Centrifugal machine |
| WO2017139864A1 (en) * | 2016-01-19 | 2017-08-24 | Shanxi Zdgsy Bio-Scientific Co., Ltd. | Multifunctional biological substance separation device |
| CN206814758U (en) * | 2017-05-27 | 2017-12-29 | 云南新生命干细胞有限公司 | A kind of stem cell filter separator |
| CN206814738U (en) * | 2017-05-27 | 2017-12-29 | 云南新生命干细胞有限公司 | A kind of cord blood stem cell separator |
| CN207391420U (en) * | 2017-10-26 | 2018-05-22 | 天津百恩生物科技有限公司 | A kind of cord blood stem cell separating bag |
| CN108060082A (en) * | 2017-12-29 | 2018-05-22 | 天津普瑞赛尔生物科技有限公司 | A kind of stem cell filter separator |
| CN208617832U (en) * | 2018-06-01 | 2019-03-19 | 湖州聚业孵化器有限公司 | A kind of stem cell extractor that extraction effect is good |
-
2018
- 2018-06-01 CN CN201810558749.6A patent/CN108504543A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203395382U (en) * | 2013-06-24 | 2014-01-15 | 江阴大田科技服务有限公司 | Hose guide rack |
| CN104056728A (en) * | 2014-07-07 | 2014-09-24 | 古廷伟 | Centrifugal machine |
| WO2017139864A1 (en) * | 2016-01-19 | 2017-08-24 | Shanxi Zdgsy Bio-Scientific Co., Ltd. | Multifunctional biological substance separation device |
| CN206814758U (en) * | 2017-05-27 | 2017-12-29 | 云南新生命干细胞有限公司 | A kind of stem cell filter separator |
| CN206814738U (en) * | 2017-05-27 | 2017-12-29 | 云南新生命干细胞有限公司 | A kind of cord blood stem cell separator |
| CN207391420U (en) * | 2017-10-26 | 2018-05-22 | 天津百恩生物科技有限公司 | A kind of cord blood stem cell separating bag |
| CN108060082A (en) * | 2017-12-29 | 2018-05-22 | 天津普瑞赛尔生物科技有限公司 | A kind of stem cell filter separator |
| CN208617832U (en) * | 2018-06-01 | 2019-03-19 | 湖州聚业孵化器有限公司 | A kind of stem cell extractor that extraction effect is good |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111808720A (en) * | 2020-07-27 | 2020-10-23 | 湖北明德健康科技有限公司 | Stem cell separation device |
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