CN111808720A - Stem cell separation device - Google Patents

Stem cell separation device Download PDF

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Publication number
CN111808720A
CN111808720A CN202010731801.0A CN202010731801A CN111808720A CN 111808720 A CN111808720 A CN 111808720A CN 202010731801 A CN202010731801 A CN 202010731801A CN 111808720 A CN111808720 A CN 111808720A
Authority
CN
China
Prior art keywords
separation
stem cell
chamber
movable
centrifugal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202010731801.0A
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Chinese (zh)
Inventor
潘飞
谢建敏
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hubei Mingde Health Technology Co ltd
Original Assignee
Hubei Mingde Health Technology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hubei Mingde Health Technology Co ltd filed Critical Hubei Mingde Health Technology Co ltd
Priority to CN202010731801.0A priority Critical patent/CN111808720A/en
Publication of CN111808720A publication Critical patent/CN111808720A/en
Pending legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04Cell isolation or sorting
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B5/00Other centrifuges
    • B04B5/10Centrifuges combined with other apparatus, e.g. electrostatic separators; Sets or systems of several centrifuges

Abstract

The invention provides a stem cell separation device, which relates to the technical field of cell extraction and separation, and comprises a base, a centrifugal mechanism and a separation and extraction mechanism, wherein a support plate is fixedly connected on the inner wall of a separation chamber, a through hole is arranged on the inner side of the support plate, a bearing is arranged in the through hole, a connecting rod is fixedly connected between a mounting plate and a rotating shaft, the rotating rod is rotatably connected at the position, close to the left side of the separation chamber, of the top of the base, a gear is arranged at the top of the rotating rod, a movable hole is arranged on the left side of the separation chamber, and a movable plate is slidably connected on the inner side of the movable hole. The method keeps a phase-closed centrifugal environment, avoids the pollution of stem cells, and improves the quality of the separated stem cells.

Description

Stem cell separation device
Technical Field
The invention relates to the technical field of cell extraction and separation, in particular to a stem cell separation device.
Background
Stem cells are a type of pluripotent cells with self-replication ability, which can be differentiated into various functional cells under certain conditions, and are classified into embryonic stem cells and adult stem cells according to the development stage of the stem cells, and are classified into three types according to the development potential of the stem cells: totipotent, pluripotent and unipotent stem cells; the stem cell is an insufficiently differentiated and immature cell, has potential functions of regenerating various tissues, organs and human bodies, and is called as a universal cell in the medical field; the stem cells are often mixed with other cells after being extracted, so a stem cell separation device is needed, and the separation step comprises the steps of firstly injecting stem cell stock solution, water and enzyme into a reaction container for reaction, and then collecting extract after the reaction is completed to complete the separation step.
However, the conventional stem cell separation device has a poor separation effect, and stem cells are exposed outside for a long time, are easily infected, affect the stem cell space, are inconvenient to extract after centrifugation, and have low efficiency.
Disclosure of Invention
The present invention is directed to a stem cell separation device to solve the above problems of the prior art.
In order to achieve the purpose, the invention provides the following technical scheme: a stem cell separation device comprises a base, a centrifugal mechanism and a separation and extraction mechanism, wherein the centrifugal mechanism comprises a motor, a rotating shaft, a connecting rod, a supporting plate, a bearing, a mounting plate, a centrifugal cylinder, a rotating rod, a gear and a movable plate, the top of the base is provided with a separation chamber, the motor is arranged at the bottom of the inner cavity of the separation chamber, the rotating shaft is fixedly connected at the output end of the motor, the supporting plate is fixedly connected on the inner wall of the separation chamber, the inner side of the supporting plate is provided with a through hole, the bearing is arranged in the through hole, the mounting plate is fixedly connected at the inner side of the bearing, the connecting rod is fixedly connected between the mounting plate and the rotating shaft, the centrifugal cylinder is fixedly connected at the middle position of the mounting plate, the rotating rod is rotatably connected at the position, close to the left, the movable plate is connected to the inner side of the movable hole in a sliding mode.
Preferably, the mechanism is drawn in the separation includes the cylinder, absorbs tubule, piston rod, handle and hangers, the top of separation chamber is equipped with the jack, cylinder swing joint is in the inboard of jack, absorb the tubule and install in the bottom of cylinder, and be located the inner chamber of centrifuge bowl, hangers fixed connection is in the outside of cylinder, and is located the top of jack, piston sliding connection is in the inner chamber of cylinder, the one end fixed connection of piston rod is at the top of piston, the other end at the piston rod is installed to the handle.
Preferably, the outer side of the rotating rod is provided with a driving wheel, the outer side of the rotating shaft is also provided with driving wheels, and a driving belt is sleeved between the two groups of driving wheels.
Preferably, a movable groove is formed in the middle of the movable plate, a tooth groove is formed in one side of the movable groove, the size of the gear is matched with that of the movable groove, a handle groove is formed in the left end of the movable plate, an opening is further formed in the right side of the movable plate, and the size of the opening is matched with that of the separating cylinder.
Preferably, the centrifugal cylinder is of a circular truncated cone shape, and the diameter of the top of the centrifugal cylinder is matched with the width of the movable plate.
Preferably, the right side of the treatment chamber is communicated with a feeding pipe, and the tail end of the feeding pipe is positioned above the centrifugal cylinder.
Compared with the prior art, the invention has the beneficial effects that:
(1) the centrifugal mechanism drives the cone-shaped centrifugal cylinder to rotate through the components such as the motor, the connecting rod and the like, so that stem cell tissue fluid is separated more quickly, extraction is facilitated, the rotating rod is driven to rotate through the driving belt, the movable plate covers the top of the centrifugal cylinder, a closed centrifugal environment is kept, stem cells are prevented from being polluted, and the quality of the separated stem cells is improved;
(2) according to the stem cell separation device, the separation cylinder is designed, the piston is driven to move through the handle, the separated stem cells are sucked and transferred into the separation cylinder, and the separation cylinder can be moved down and transferred, so that the efficiency and the quality of stem cell separation are improved.
Of course, it is not necessary for any product in which the invention is practiced to achieve all of the above-described advantages at the same time.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of a movable plate according to the present invention;
FIG. 3 is a schematic top view of the supporting plate of the present invention.
In the drawings, the components represented by the respective reference numerals are listed below:
1-base, 2-centrifugal mechanism, 201-motor, 202-rotating shaft, 203-connecting rod, 204-supporting plate, 205-bearing, 206-mounting plate, 207-centrifugal cylinder, 208-rotating rod, 209-gear, 210-movable plate, 3-separation and extraction mechanism, 301-separation cylinder, 302-suction tubule, 303-piston, 304-piston rod, 305-handle, 306-suspension loop, 4-separation chamber, 5-movable hole, 6-jack, 7-driving wheel, 8-driving belt, 9-movable groove, 10-tooth groove, 11-handle groove, 12-opening and 13-feeding pipe.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-3, the present invention provides a technical solution: a stem cell separation device comprises a base 1, a centrifugal mechanism 2 and a separation and extraction mechanism 3, wherein the centrifugal mechanism 2 comprises a motor 201, a rotating shaft 202, a connecting rod 203, a supporting plate 204, a bearing 205, a mounting plate 206, a centrifugal cylinder 207, a rotating rod 208, a gear 209 and a movable plate 210, the top of the base 1 is provided with a separation chamber 4, the motor 201 is arranged at the bottom of an inner cavity of the separation chamber 4, the rotating shaft 202 is fixedly connected with the output end of the motor 201, the supporting plate 204 is fixedly connected on the inner wall of the separation chamber 4, the inner side of the supporting plate 204 is provided with a through hole, the bearing 205 is arranged in the through hole, the mounting plate 206 is fixedly connected on the inner side of the bearing 205, the connecting rod 203 is fixedly connected between the mounting plate 206 and the rotating shaft 202, the centrifugal cylinder 207 is fixedly connected at the middle position of the mounting plate, the left side of the separation chamber 4 is provided with a movable hole 5, and the movable plate 5 is connected to the inner side of the movable hole in a sliding way.
Wherein, the mechanism 3 is drawed in the separation includes cylinder 301, absorb tubule 302, piston 303, piston rod 304, handle 305 and hangers 306, the top of separation chamber 4 is equipped with jack 6, cylinder 301 swing joint is in the inboard of jack 6, absorb tubule 302 and install the bottom at cylinder 301, and be located the inner chamber of centrifuge tube 207, hangers 306 fixed connection is in the outside of cylinder 301, and be located jack 6's top, piston 303 sliding connection is in the inner chamber of cylinder 301, the one end fixed connection of piston rod 304 is at the top of piston 303, the other end at piston rod 304 is installed to handle 305.
Wherein, the outer side of the rotating rod 208 is provided with a driving wheel 7, the outer side of the rotating shaft 202 is also provided with the driving wheels 7, and a driving belt 8 is sleeved between the two groups of driving wheels 7.
Wherein, the middle position of the movable plate 210 is provided with a movable groove 9, one side of the movable groove 9 is provided with a tooth socket 10, the size of the gear 209 is matched with the movable groove 9, the left end of the movable plate 210 is provided with a handle groove 11, the right side of the movable plate 210 is also provided with an opening 12, and the size of the opening 12 is matched with the size of the separation cylinder 301.
Wherein, the centrifugal cylinder 207 is of a truncated cone shape, and the diameter of the top of the centrifugal cylinder 207 matches with the width of the movable plate 210.
Wherein, the right side of the processing chamber 4 is communicated with a feeding pipe 13, and the end of the feeding pipe 13 is positioned above the centrifugal cylinder 207.
One specific application of this embodiment is: the device supplies electric energy to electrical elements in the device through an external power supply and controls the on-off of the electrical elements through a control switch;
when the centrifugal separation device is used, firstly, stem cell tissue fluid is injected into the separation cylinder 301 of the separation chamber 4 through the feeding pipe 13, then, the motor 201 is started, the motor 201 rotates to drive the mounting plate 206 to rotate, so that the centrifugal cylinder 207 starts to rotate, meanwhile, the rotating rod 208 rotates under the action of the driving wheel 7 and the driving belt 8, the gear 209 rotates along with the rotating rod, the movable plate 210 is driven to move rightwards, the top of the separation cylinder 301 is covered, so that the separation cylinder is kept closed, when the gear 209 moves to the left end of the movable groove 9, the gear is separated from the tooth space 10, at the moment, the movable plate 210 stops moving, the rotating speed of the motor 201 can be slightly increased, so that the separation oscillation effect of the separation cylinder 301 is better, after separation is completed, the piston 303 moves upwards by pulling the handle 305, the stem cells at the bottom layer are sucked into the separation cylinder 301.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims (6)

1. A stem cell separation device, comprising: comprises a base (1), a centrifugal mechanism (2) and a separation extraction mechanism (3), wherein the centrifugal mechanism (2) comprises a motor (201), a rotating shaft (202), a connecting rod (203), a supporting plate (204), a bearing (205), a mounting plate (206), a centrifugal cylinder (207), a rotating rod (208), a gear (209) and a movable plate (210), a separation chamber (4) is arranged at the top of the base (1), the motor (201) is arranged at the bottom of the inner cavity of the separation chamber (4), the rotating shaft (202) is fixedly connected at the output end of the motor (201), the supporting plate (204) is fixedly connected on the inner wall of the separation chamber (4), a through hole is arranged at the inner side of the supporting plate (204), the bearing (205) is arranged in the through hole, the mounting plate (206) is fixedly connected at the inner side of the bearing (205), the connecting rod (203) is fixedly connected between the mounting plate (206) and, centrifugal cylinder (207) fixed connection is in the intermediate position of mounting panel (206), bull stick (208) are rotated and are connected and are close to the left position of separation chamber (4) at base (1) top, the top at bull stick (208) is installed in gear (209), the left side of separation chamber (4) is equipped with movable hole (5), fly leaf (5) sliding connection is in the inboard of movable hole.
2. A stem cell separation device according to claim 1, wherein: mechanism (3) are drawed in the separation includes knockout drum (301), absorbs tubule (302), piston (303), piston rod (304), handle (305) and hangers (306), the top of separator (4) is equipped with jack (6), knockout drum (301) swing joint is in the inboard of jack (6), absorb tubule (302) and install the bottom at knockout drum (301), and be located the inner chamber of centrifuge tube (207), hangers (306) fixed connection is in the outside of knockout drum (301), and is located the top of jack (6), piston (303) sliding connection is at the inner chamber of knockout drum (301), the one end fixed connection of piston rod (304) is at the top of piston (303), the other end at piston rod (304) is installed in handle (305).
3. A stem cell separation device according to claim 1, wherein: the outer side of the rotating rod (208) is provided with a driving wheel (7), the outer side of the rotating shaft (202) is also provided with the driving wheel (7), and a driving belt (8) is sleeved between the two groups of driving wheels (7).
4. A stem cell separation device according to claim 2, wherein: the intermediate position of fly leaf (210) is equipped with movable groove 9, one side of movable groove (9) is equipped with tooth's socket (10), the size and the movable groove (9) phase-match of gear (209), the left end of fly leaf (210) is equipped with handle groove (11), the right side of fly leaf (210) still is equipped with opening (12), the size of opening (12) and the size phase-match of a separation section of thick bamboo (301).
5. A stem cell separation device according to claim 1, wherein: the centrifugal cylinder (207) is in a circular truncated cone shape, and the diameter of the top of the centrifugal cylinder (207) is matched with the width of the movable plate (210).
6. A stem cell separation device according to claim 1, wherein: the right side of the treatment chamber (4) is communicated with a feeding pipe (13), and the tail end of the feeding pipe (13) is positioned above the centrifugal cylinder (207).
CN202010731801.0A 2020-07-27 2020-07-27 Stem cell separation device Pending CN111808720A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010731801.0A CN111808720A (en) 2020-07-27 2020-07-27 Stem cell separation device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010731801.0A CN111808720A (en) 2020-07-27 2020-07-27 Stem cell separation device

Publications (1)

Publication Number Publication Date
CN111808720A true CN111808720A (en) 2020-10-23

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Application Number Title Priority Date Filing Date
CN202010731801.0A Pending CN111808720A (en) 2020-07-27 2020-07-27 Stem cell separation device

Country Status (1)

Country Link
CN (1) CN111808720A (en)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0446450A1 (en) * 1990-02-09 1991-09-18 Thomas Jefferson University Device for collecting and processing fat tissue to produce endothelial cell product
EP2943562A1 (en) * 2013-03-04 2015-11-18 Swiss Stem Cell Foundation A system for extraction of cells from a sample of tissue
CN107048770A (en) * 2017-04-21 2017-08-18 何朋飞 It is a kind of effectively to place the rack of bellows
CN108504543A (en) * 2018-06-01 2018-09-07 湖州聚业孵化器有限公司 A kind of stem cell extractor that extraction effect is good
CN208788044U (en) * 2018-09-26 2019-04-26 江西凯安智能股份有限公司 A kind of copper bar cutter device
CN209076739U (en) * 2018-11-12 2019-07-09 湖南博隆矿业开发有限公司 A kind of ore processing device
CN110777051A (en) * 2019-12-02 2020-02-11 安徽惠恩生物科技股份有限公司 Centrifugal separation and extraction device for tissue stem cells

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0446450A1 (en) * 1990-02-09 1991-09-18 Thomas Jefferson University Device for collecting and processing fat tissue to produce endothelial cell product
EP2943562A1 (en) * 2013-03-04 2015-11-18 Swiss Stem Cell Foundation A system for extraction of cells from a sample of tissue
CN107048770A (en) * 2017-04-21 2017-08-18 何朋飞 It is a kind of effectively to place the rack of bellows
CN108504543A (en) * 2018-06-01 2018-09-07 湖州聚业孵化器有限公司 A kind of stem cell extractor that extraction effect is good
CN208788044U (en) * 2018-09-26 2019-04-26 江西凯安智能股份有限公司 A kind of copper bar cutter device
CN209076739U (en) * 2018-11-12 2019-07-09 湖南博隆矿业开发有限公司 A kind of ore processing device
CN110777051A (en) * 2019-12-02 2020-02-11 安徽惠恩生物科技股份有限公司 Centrifugal separation and extraction device for tissue stem cells

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Application publication date: 20201023

RJ01 Rejection of invention patent application after publication