CN207259496U - Nucleic acid hybridization oven - Google Patents
Nucleic acid hybridization oven Download PDFInfo
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- CN207259496U CN207259496U CN201721200708.7U CN201721200708U CN207259496U CN 207259496 U CN207259496 U CN 207259496U CN 201721200708 U CN201721200708 U CN 201721200708U CN 207259496 U CN207259496 U CN 207259496U
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Abstract
The utility model discloses a kind of nucleic acid hybridization oven, it includes board, pipetting device, washes model machine structure, pump liquid mechanism and control mechanism.The nucleic acid hybridization oven automatically controls sample-adding driving device by control mechanism, washes sample driving device and pump liquid mechanism action, reagent, automatic incubation, automatic cleaning can be added automatically, the whole process that is incubated is without manual intervention, it can be automatically performed, it is time saving and energy saving, and advantageously ensure that the uniformity of detection process, and then it is advantageously implemented normalizing operation, repeatability and the high throughput of nucleic acid hybridization in situ experiment.
Description
Technical field
Molecular Biological Detection field is the utility model is related to, more particularly, to a kind of nucleic acid hybridization oven.
Background technology
It is miscellaneous that RNA In-stitu hybridizations (RNA nucleic acid hybridization in situ) are also known as RNA original positions
Hand over histochemistry (RNAin situ hybridization histochemistry) or RNA in situ hybridizations (RNA in situ
hybridization RISH).The technology is a kind of with RNA tables in the probe in detecting cells such as cRNA or oligonucleotides and tissue
The hybridization in situ technique reached.Its basic principle is：Under conditions of cell or tissue structure remains unchanged, known to mark
RNA nucleotide fragments, by basepairing rule, are combined with corresponding genetic fragment in cell or tissue to be measured, are formed
Crossbred is after chromogenic reaction in light microscope or observed under electron microscope.RNA hybridization in situ technique through continuously improving, its
DNA hybridization in situ technique is far led in the field of application.It is as one of most effective molecular pathology technology, in gene point
It can make qualitative, positioning and quantitative analysis in terms of analysis and diagnosis, while can also effectively analyze low abundance and rare mRNA expression.
Although RNA hybridization in situ technique is a kind of common molecular detection technology, the technical step is cumbersome, time-consuming to take
Power, including many simple but cleaning that repeats and is incubated process, and manual operation face it is uninteresting it is cumbersome, take time and effort
The problems such as, and it is difficult to ensure that the uniformity of whole detection process, often influence accuracy and the confidence level of result.
Utility model content
Based on this, it is necessary to provide a kind of nucleic acid hybridization oven, taken with solving traditional RNA when nucleic acid hybridization procedure
Power and the problem of be difficult to ensure that detection process uniformity.
The technical solution that the utility model solves above-mentioned technical problem is as follows.
A kind of nucleic acid hybridization oven, including：
Board, the board have reagent area, Xi Zhen areas, are incubated area and washing lotion area, and the reagent area is used to place reagent,
The Xi Zhen areas, which have, washes pin pond, and the area that is incubated has the incubation slot for being used for placing response container, and the washing lotion area is used to put
Put cleaning solution；
Pipetting device, including sample needle and sample-adding driving device, it is described sample-adding driving device be located on the board and with
The sample needle connection moves for the driving sample needle between the reagent area, the Xi Zhen areas and the incubation area
It is dynamic；
Wash model machine structure, including injection needle, aspirating needle and wash sample driving device, the sample driving device of washing is located at the board
Above and with the injection needle and the aspirating needle it is connected respectively for driving the injection needle and the aspirating needle to be washed described
Moved between pin area and the incubation area；
Pump liquid mechanism, is connected respectively with the sample needle, pin pond, the injection needle and the aspirating needle washed；And
Control mechanism, with the sample-adding driving device, described wash sample driving device and the pump liquid mechanism is connected；
The control mechanism is used to control the sample-adding driving device that the sample needle is moved to the reagent area, and controls
The pump liquid mechanism is made via the sample needle from the reagent area pumping reagent；The control mechanism is additionally operable to add described in control
The sample needle is moved to the incubation area by sample driving device, and controls the pump liquid mechanism by the reagent in the sample needle
Pump out to the reaction vessel in the incubation slot；The control mechanism is additionally operable to control the sample-adding driving device described will add
Sample pin be moved to it is described wash in pin pond, and control the pump liquid mechanism from the cleaning solution in the washing lotion area pumping cleaning solution with point
The inner and outer wall of the sample needle is not cleaned；The control mechanism is additionally operable to control the sample-adding driving device by the sample-adding
Pin resets；The control mechanism is additionally operable to control the pump liquid mechanism to pump out the cleaning waste liquid washed in pin pond；The control
Mechanism processed is additionally operable to wash sample driving device described in control the injection needle is moved to the incubation area, and controls the pump liquid machine
Structure pumping cleaning solution and is pumped out into the reaction vessel from the cleaning solution in the washing lotion area by the injection needle；The control
Mechanism processed is additionally operable to wash sample driving device described in control the aspirating needle is moved to the incubation area, and controls the pump liquid machine
Structure pumps out the cleaning waste liquid in the reaction vessel via the aspirating needle；The control mechanism is additionally operable to wash sample described in control
Driving device by the injection needle and the aspirating needle be respectively moved to it is described wash in pin pond, and control the pump liquid mechanism from institute
Pumping cleaning solution is stated in the cleaning solution in washing lotion area to be respectively washed the injection needle and the aspirating needle；The control mechanism is also used
The injection needle and the aspirating needle is driven to reset in washing sample driving device described in control.
In one of the embodiments, the reagent area has reagent rack；The bottom of the reagent rack has the first heating
Device, the first heater are connected with the control mechanism.
In one of the embodiments, the bottom of the incubation slot is equipped with secondary heating mechanism, the secondary heating mechanism
It is connected with the control mechanism；And/or
The bottom of the incubation slot is equipped with jacking mechanism, and the jacking mechanism is connected with by the control with the control mechanism
The side of the reaction vessel in the incubation slot is jacked up to form inclination by mechanism controls processed；And/or
Temperature sensor is equipped with the incubation slot, the temperature sensor is electrically connected with the control mechanism；And/or
The top of the incubation slot is equipped with box lid.
In one of the embodiments, the nucleic acid hybridization oven further includes the box lid driving dress being connected with the control mechanism
Put, the box lid driving device is connected with the box lid for by driving the box lid to move incubation described in open or close
Groove.
In one of the embodiments, the washing lotion area is equipped with multiple bottle for handling liquid toilet or cosmetic substance, multiple bottle for handling liquid toilet or cosmetic substance respectively by pipeline with
The pump liquid mechanism connection；Each independent control valve, the control valve and institute are equipped with the corresponding pipeline of the bottle for handling liquid toilet or cosmetic substance
Control mechanism connection is stated to be opened or closed by control mechanism control.
In one of the embodiments, the injection needle and the aspirating needle are set in a row respectively, and rows of multiple institutes
Injection needle is stated to be arranged at intervals with rows of multiple aspirating needles.
In one of the embodiments, the pump liquid mechanism includes plunger pump and the first diaphragm pump, the plunger pump and institute
State the first diaphragm pump to concatenate with the sample needle, the plunger pump is used to control the sample needle to try from the reagent area pumping
Agent and the control sample needle pump out the reagent of pumping into the reaction vessel, and first diaphragm pump is used for from described
Washing lotion area pumping cleaning solution is simultaneously pumped into the sample needle and pumps out to described and wash in pin pond to clean in the sample needle
Wall and outer wall.
In one of the embodiments, the pump liquid mechanism further includes the second diaphragm pump, second diaphragm pump with it is described
Pin pond concatenation is washed, second diaphragm pump is used to pump out the waste liquid for washing pin pond.
In one of the embodiments, the pump liquid mechanism further includes the 3rd diaphragm pump and the 4th diaphragm pump, and the described 3rd
Diaphragm pump is concatenated with the injection needle to pump out from the washing lotion area pumping cleaning solution and via the injection needle, the described 4th every
Membrane pump is concatenated with the aspirating needle with by the aspirating needle, pumping is cleaned waste liquid and pumped out out of described reaction vessel.
In one of the embodiments, the nucleic acid hybridization oven further includes the waste liquid bottle interface being located on the board, institute
Waste liquid bottle interface is stated to be connected with second diaphragm pump and the 4th diaphragm pump.
Above-mentioned nucleic acid hybridization oven automatically controls sample-adding driving device by control mechanism, washes sample driving device and pump liquid mechanism
Action, can add automatically reagent, it is automatic be incubated, automatic cleaning, the whole process that is incubated can be automatically performed without manual intervention,
It is time saving and energy saving, and advantageously ensure that the uniformity of detection process, and then it is advantageously implemented the standardization of nucleic acid hybridization in situ experiment
Operation, repeatability and high throughput.
Brief description of the drawings
Fig. 1 is the structure diagram of the incubation slot open mode of the nucleic acid hybridization oven of an embodiment；
Fig. 2 is the structure diagram of the incubation slot closed mode of nucleic acid hybridization oven shown in Fig. 1；
Fig. 3 is the top view of nucleic acid hybridization oven shown in Fig. 2；
Fig. 4 is the structure diagram that driving device is loaded in Fig. 1；
Fig. 5 is the structure diagram that sample driving device is washed in Fig. 1；
Fig. 6 is the structure diagram of part-structure in nucleic acid hybridization oven shown in Fig. 1；
Fig. 7 is the structure diagram of jacking mechanism in Fig. 1；
Fig. 8 is the cooperation schematic diagram of jacking mechanism shown in Fig. 7 and the part-structure shown in Fig. 6；
Fig. 9 is the complete machine schematic diagram of nucleic acid hybridization oven shown in Fig. 1；
Figure 10 is the liquid channel system structural scheme of mechanism of nucleic acid hybridization oven shown in Fig. 1；
Figure 11 is injection needle in Fig. 1, the structure diagram of aspirating needle and needle stand；
Figure 12 is the structure diagram at another visual angle of injection needle shown in Figure 11, aspirating needle and needle stand.
Embodiment
For the ease of understanding the utility model, the utility model is more fully retouched below with reference to relevant drawings
State.The preferred embodiment of the utility model is given in attached drawing.But the utility model can come in fact in many different forms
It is existing, however it is not limited to embodiment described herein.On the contrary, the purpose for providing these embodiments is the public affairs made to the utility model
Open the understanding more thorough and comprehensive of content.
It should be noted that when element is referred to as " being fixed on " another element, it can be directly on another element
Or there may also be element placed in the middle.When an element is considered as " connection " another element, it can be directly connected to
To another element or it may be simultaneously present centering elements.
Unless otherwise defined, all of technologies and scientific terms used here by the article is led with belonging to the technology of the utility model
The normally understood implication of technical staff in domain is identical.It is simply in the term used in the description of the utility model herein
The purpose of description specific embodiment, it is not intended that in limitation the utility model.Term as used herein "and/or" includes
The arbitrary and all combination of one or more relevant Listed Items.
Incorporated by reference to Fig. 1, Fig. 2 and Fig. 3, the nucleic acid hybridization oven 10 of an embodiment includes board 100, pipetting device 200, washes
Model machine structure 300, pump liquid mechanism 400 and control mechanism 500.
Board 100 has reagent area 102, Xi Zhen areas 104, is incubated area 106 and washing lotion area 108.Reagent area 102 is used to place
Reagent.Xi Zhen areas 104, which have, washes pin pond 110.Being incubated area 106 has the incubation slot 120 for being used for placing response container 20.Washing lotion area
108 are used to place cleaning solution.
Pipetting device 200 includes sample needle 210 and sample-adding driving device 220.Sample-adding driving device 220 is located at board 100
Above and with sample needle 210 it is connected and is moved for driving sample needle 210 between reagent area 102, Xi Zhen areas 104 and incubation area 106
It is dynamic.
In one embodiment, being loaded driving device 220 includes sample-adding guide rail, sample-adding sliding block and sample-adding driving motor.Add
Sample guide rail, sample-adding sliding block and sample-adding driving motor form sample arm group.Sample needle 210 can by sample arm group drive it is forward and backward,
Left and right and upper and lower movement is (i.e. in three-dimensional arbitrary motion).Sample-adding driving motor preferably uses stepper motor, control accuracy
It is high.
Specifically, Fig. 4 please be join, in the embodiment shown in fig. 4, sample-adding guide rail includes X-axis sample-adding guide rail 221, Y-axis sample-adding is led
Rail 222 and Z axis sample-adding guide rail 223；Being loaded sliding block includes X-axis sample-adding sliding block 224, Y-axis sample-adding sliding block 225 and Z axis sample-adding sliding block
226；Correspondingly, sample-adding driving motor includes X-axis sample-adding motor 227, Y-axis sample-adding motor 228 and Z axis sample-adding motor 229.Y-axis
Sample-adding guide rail 222, Y-axis sample-adding sliding block 225 and Y-axis sample-adding motor 228 are integrally provided on X-axis sample-adding sliding block 224, by X-axis plus
The action of sample motor 227 can drive Y-axis sample-adding guide rail 222, Y-axis sample-adding sliding block 225 and 228 entirety of Y-axis sample-adding motor to be moved along X-axis
It is dynamic；Z axis sample-adding guide rail 223, Z axis sample-adding sliding block 226 and Z axis sample-adding motor 229 are integrally provided on Y-axis sample-adding sliding block 225, are led to
Crossing the Y-axis sample-adding action of motor 228 can drive Z axis sample-adding guide rail 223, Z axis sample-adding sliding block 226 and Z axis sample-adding motor 229 overall
Moved along Y-axis；Sample needle 210 sets Z axis to be loaded on sliding block 226, and being loaded motor 229 by Z axis acts, and can realize in Z axis
Move up and down.
More specifically, in the embodiment shown in fig. 4, sample-adding sliding block (such as X-axis sample-adding sliding block 224, Y-axis sample-adding sliding block 225 and
Z axis is loaded sliding block 226) (such as X-axis is loaded motor 227, Y-axis sample-adding motor 228 and Z axis sample-adding motor with sample-adding driving motor
229) it is sequentially connected between by transmission belt, sample-adding sliding block is fixed on the side of transmission belt, and sample-adding driving motor rotates being capable of band
The transmission belt is moved to rotate and then drive sample-adding slide block movement.
Washing model machine structure 300 includes injection needle 310, aspirating needle 320 and washes sample driving device 330.Sample driving device 330 is washed to set
It is connected on board 100 and respectively with injection needle 310 and aspirating needle 320 and is moved for driving injection needle 310 and aspirating needle 320
To incubation area 106.
In one embodiment, washing sample driving device 330 includes washing sample guide rail, wash sample sliding block and washing sample driving motor etc..
Wash sample guide rail and wash sample driving motor composition and wash sample arm group.Injection needle 310, aspirating needle 320 can be forward and backward by washing the drive of sample arm group
And upper and lower movement.Wash sample driving motor and preferably use stepper motor, control accuracy is high.
Specifically, Fig. 5 please be join, in the embodiment shown in fig. 5, wash that sample guide rail washes sample guide rail 331 including Y-axis and Z axis is washed
Sample guide rail 332；Wash that sample sliding block washes sample sliding block 333 including Y-axis and Z axis washes sample sliding block 334；Wash sample driving motor and wash sample including Y-axis
Motor 335 and Z axis wash sample motor 336.Z axis washes sample guide rail 332, Z axis washes sample sliding block 334 and Z axis washes sample motor 336 and is located at Y-axis
Wash on sample sliding block 333,335 drives edge Y-axis of sample motor can be washed by Y-axis and washes the 331 forward and backward movement of sample guide rail.Injection needle 310, inhale
Liquid pin 320 is located at Z axis and washes on sample sliding block 334, and 336 drives edge Z axis of sample motor can be washed by Z axis and washes the 332 upper and lower shifting of sample guide rail
It is dynamic.
Further, in the embodiment shown in fig. 5, Y-axis wash sample sliding block 333 and Y-axis wash sample motor 335 between pass through transmission
V belt translation connects, and Y-axis washes the side that sample sliding block 333 is fixed on transmission belt, and Y-axis, which washes the rotation of sample motor 335, can drive the transmission
Band rotates and then drives Y-axis to wash sample sliding block 333 and moves.Z axis washes sample motor 336 and washes sample sliding block by screw rod transmission component and Z axis
334 connections, drive Z axis to wash sample sliding block 334 and move up and down by screw rod transmission principle.
It is understood that in other embodiments, it is loaded between sliding block and sample-adding driving motor and washes sample sliding block and wash sample drive
Dynamic motor is not limited by the transmission of the transmission mechanisms such as above-mentioned transmission belt, screw rod transmission component.
Pump liquid mechanism 400 and sample needle 210, wash pin pond 110, injection needle 310 and aspirating needle 320 and be connected respectively.
Control mechanism 500 and sample-adding driving device 220, wash sample driving device 330 and pump liquid mechanism 400 is connected, with respectively
Control sample-adding driving device 220, wash sample driving device 330 and pump liquid mechanism 400 acts.
Specifically, in the present embodiment, control mechanism 500 is used to control sample-adding driving device 220 to move sample needle 210
Move to reagent area 102, and control pump liquid mechanism 400 via sample needle 210 from 102 pumping reagent of reagent area.Control mechanism 500 is also
Area 106 is incubated for controlling sample-adding driving device 220 to be moved to sample needle 210, and controls pump liquid mechanism 400 by sample needle
Reagent in 210 is pumped out to the reaction vessel in incubation slot 120.Control mechanism 500 is additionally operable to control sample-adding driving device 220
Sample needle 210 is moved to and is washed in pin pond 110, and controls the pumping cleaning solution from the cleaning solution in washing lotion area 108 of pump liquid mechanism 400
To be respectively washed the inner and outer wall of sample needle 210.Control mechanism 500 is additionally operable to control sample-adding driving device 220 by sample needle
210 reset.Control mechanism 500 is additionally operable to control pump liquid mechanism 400 and pumps out the cleaning waste liquid washed in pin pond 110.Control mechanism
500, which are additionally operable to control, washes sample driving device 330 and is moved to injection needle 310 and be incubated area 106, and controls pump liquid mechanism 400 from washing
Pumping cleaning solution and pumped out in the cleaning solution of liquid zone 108 by injection needle 310 into reaction vessel.Control mechanism 500 is additionally operable to
Control washes sample driving device 330 and aspirating needle 320 is moved to incubation area 106, and controls pump liquid mechanism 400 by reaction vessel
Cleaning waste liquid is pumped out via aspirating needle 320.Control mechanism 500 is additionally operable to control and washes sample driving device 330 by injection needle 310 and inhale
Liquid pin 320 is respectively moved to wash in pin pond 110, and controls the pumping cleaning solution from the cleaning solution in washing lotion area 108 of pump liquid mechanism 400
To be respectively washed injection needle 310 and aspirating needle 320.Control mechanism 500 is additionally operable to control and washes the drive injection needle of sample driving device 330
310 and aspirating needle 320 reset.
In one embodiment, reagent area 102 has reagent rack 130.Reagent bottle can be placed in the reagent rack 130.It is excellent
Choosing, reagent rack 130 have it is multiple, for placing various sizes of reagent bottle.
In one embodiment, the bottom of the reagent rack 130 is equipped with first heater (not shown).First heater
It is connected with control mechanism 500, for being heated to the reagent bottle 30 placed in reagent rack 130, to control examination to be added
The temperature of agent.
The top of incubation slot 120 is equipped with box lid 122.As shown in Fig. 5, Fig. 6 and Fig. 7, in a specific embodiment, box
Lid 122 is slidably located on box lid slide 124, and is also correspondingly provided with box lid driving device 126.Box lid driving device 126 is used
Moved in driving box lid 122 along box lid slide 124, to close or open incubation slot 120.More specifically, in this embodiment, box
Lid driving device 126 is sequentially connected by belt drive component 128 and box lid 122.Further, the back side of box lid 122 is pasted
There are insulation material layer, such as EVA layer, it is possible to reduce heat dissipates, liquid evaporation when closing incubation slot 120 is incubated to reduce.
In one embodiment, the bottom of incubation slot 120 is equipped with secondary heating mechanism (not shown).Secondary heating mechanism with
Control mechanism 500 connects, and to maintain or control in real time the temperature in incubation slot 120, ensure incubation process stablizes progress.Into one
Step, incubation slot 120 is interior to be equipped with temperature sensor.Temperature sensor is electrically connected with control mechanism 500, to monitor incubation slot in real time
Temperature in 120.
In addition, in one embodiment, the bottom of incubation slot 120 is additionally provided with jacking mechanism.Jacking mechanism and control mechanism
500 connections form inclination to control to jack up the side of the reaction vessel 20 in incubation slot by control mechanism 500, in order to imbibition
320 imbibition of pin.It please join Fig. 6, Fig. 7 and Fig. 8, in a specific embodiment, incubation slot 120 has two, each incubation
A reaction vessel 20 can be placed in groove 120.Accordingly, jacking mechanism is included in the bottom setting of each incubation slot 120
Two fore-sets 121.Four fore-sets 121 are equipped with two incubation slots 120 altogether.Each 121 independent control in the vertical direction of fore-set
It is scalable, if fore-set 121 can be the output shaft of telescopic cylinder, can also be driven respectively by driving motor positive and inverse rising or under
Drop etc..Two fore-sets 121 in each incubation slot 120 correspond to the both sides of reaction vessel 20 respectively, can stretch respectively, and then can be real
Existing reaction vessel 20 waves inclination, is conducive to being sufficiently mixed for Incubating Solution.
It please join Fig. 9 and Figure 10, in one embodiment, washing lotion area 108 is equipped with multiple bottle for handling liquid toilet or cosmetic substance 40.Multiple bottle for handling liquid toilet or cosmetic substance 40 divide
It is not connected by pipeline with pump liquid mechanism 400.Independent control valve 460 is equipped with 40 corresponding pipeline of each bottle for handling liquid toilet or cosmetic substance, is controlled
Valve 460 processed is preferably connected with control mechanism, with the on-off of each pipeline of automatic and independent control by control mechanism, easy to pumping not
Same cleaning solution.
In one embodiment, reaction vessel 20 be preferably porous plate, correspondingly, the size and shape of incubation slot 120 with
Porous plate is corresponding.Correspondingly, injection needle 310 and aspirating needle 320 are set in a row respectively, and rows of multiple injection needles
310 are arranged at intervals with rows of multiple aspirating needles 320.It is parallel horizontal stroke for the injection needle 310 and aspirating needle 320 set in a row
Row is set, such as all horizontally-arranged along X-axis.Injection needle 310 is one high and one low with aspirating needle 320 and one thick one is thin, specifically, the note of first row
Liquid pin 310 is thinner and slightly shorter, and the aspirating needle 320 of second row is relatively thick and slightly long.Injection needle 310 is set in a row with aspirating needle 320, can
Efficiently advantageously to carry out the addition and sucking-off of cleaning solution.
As is illustrated by figs. 11 and 12, in a specific embodiment, reaction vessel 20 is 24 orifice plates, correspondingly, fluid injection
Pin 310 sets in a row and is located on needle stand 340 respectively with aspirating needle 320, and often row has 6 injection needles 310 or 6 aspirating needles 320,6
A injection needle 310 or 6 aspirating needles 320 are arranged at intervals.Needle stand 340 is equipped with two pipe joints 342, two pipe joints
342 are connected with injection needle 310 with aspirating needle 320 respectively by the independent flow passage inside needle stand 340.
In one embodiment, Xi Zhen areas 104 have two, and each Xi Zhen areas 104 are equipped with one and wash pin pond 110.Wherein
One to wash 110 size of pin pond smaller, is set close to reagent area 102, to applied to cleaning sample needle 210, another washes pin pond 110
Size is larger, the rear for being incubated area 106 is correspondingly arranged at, for cleaning injection needle 310 and aspirating needle 320.Wash sample driving device 330
Injection needle 310 and aspirating needle 320 can be driven to be incubated area 106 and moved in washing between pin pond 110 of being incubated that 106 rear of area sets
It is dynamic, in embodiment as shown in fig. 5, can be washed by Y-axis sample motor 335 drive be incubated area 106 with it is corresponding wash pin pond 110 it
Between move.As shown in Figure 10, in one embodiment, pump liquid mechanism 400 includes 450 and first diaphragm pump 410 of plunger pump.Plunger
450 and first diaphragm pump 410 of pump is concatenated with sample needle 210.Plunger pump 450 is used to control sample needle 210 to pump from reagent area 102
Reagent and control sample needle 210 is taken to pump out the reagent of pumping into reaction vessel 20.First diaphragm pump 410 is used for from washing lotion
108 pumping cleaning solution of area and be pumped into sample needle 210 and pump out to wash in pin pond 110 with clean the inner wall of sample needle 210 and
Outer wall.
Further, in one embodiment, pump liquid mechanism 400 further includes the second diaphragm pump 420.Second diaphragm pump 420 with
Pin pond 110 is washed to concatenate.Second diaphragm pump 420 is used to pump out the waste liquid for washing pin pond 110.
Further, in one embodiment, pump liquid mechanism 400 further includes the 3rd diaphragm pump 430 and the 4th diaphragm pump
440.3rd diaphragm pump 430 is concatenated with injection needle 310 to pump out from 108 pumping cleaning solution of washing lotion area and via injection needle 310.The
Four diaphragm pumps 440 are connected with aspirating needle 320 with by aspirating needle 320, pumping is cleaned waste liquid and pumped out out of reaction vessel.
In one embodiment, which further includes the waste liquid bottle interface 600 being located on board 100.Waste liquid
Bottle interface 600 is connected with the second diaphragm pump 420 and the 4th diaphragm pump 440, will cleaning waste liquid discharge.
In addition, as shown in figure 9, in a specific embodiment, board 100 is equipped with cover body 140, and cover body 140 is by each member
Part covers on inside.Cover body 140 is equipped with the first protective door 142 and the second protective door 144., can be after first protective door 142 is opened
Reagent is placed in reagent area 102, is being incubated 106 placing response container 20 of area etc.；After second protective door 144 is opened, washing lotion can be put into
Bottle 40.By setting cover body 140, it can effectively prevent that ensure experimental implementation can to being incubated the influence of experimentation for extraneous factor
By property.
In the embodiment of a preferable nucleic acid hybridization oven, the workflow when carrying out in situ hybridization is as follows：
1. reagent adds：Sample arm group drives suction needle 210 to move, and suction needle 210 is moved to the examination in reagent rack 130
In agent bottle 30, plunger pump 450 acts, and draws quantitative reagent.Then sample arm group drives suction needle 210 to be moved to incubation slot 120
On interior sample, plunger pump 450 moves, and reagent is released suction needle 210, is added on sample.
2. suction needle 210 cleans：Sample arm group drives suction needle 210 to be inserted into and washes in pin pond 110, startup and suction needle
First diaphragm pump 410 of 210 connections, pumping cleaning solution clean the inner wall of suction needle 210, and cleaning solution drains into wash pin at the same time
Cleaned in pond 110 with the outer wall to suction needle 210, cleaning waste liquid flow to and washes pin pond 110, restarts and washes pin pond 110 and connects
The second diaphragm pump 420 connect, waste liquid barrel is pumped into using the second diaphragm pump 420 by waste liquid is cleaned via waste liquid bottle interface 600.
3. it is incubated：When reaction vessel 20 is incubated in incubation slot 120, box lid 122 will be skidded off to cover and entirely incubated automatically
Educate groove.The program of incubation at room temperature is adjusted without temperature, needs the program of incubation with heat under the control of control mechanism 500, incubation slot
120 bottoms carry out the step after preheating is warming up to assigned temperature.
4. cleaning solution adds：Cleaning solution addition by washes model machine structure 300 carry out liquid feeding, with for reaction vessel 20 for 24 holes
Exemplified by the nucleic acid hybridization oven of plate, wash model machine structure 300 has six injection needles 310 side by side, washes sample arm group and drives injection needle 310 to be moved to
Directly over 24 orifice plate, the connection with corresponding bottle for handling liquid toilet or cosmetic substance 40 is realized by 460 door switch of control valve, restarts the 3rd diaphragm pump 430,
Quantitative cleaning solution is drawn in 24 orifice plates by six injection needles 310 under the drive of the 3rd diaphragm pump 430, is finally washing sample arm group
Injection needle 310 is washed sample row's pin under the drive of band to set back.
5. cleaning solution removes：Cleaning solution is removed and absorbed by aspirating needle 320, is 24 orifice plates with above-mentioned reaction vessel 20
Nucleic acid hybridization oven exemplified by, wash model machine structure 300 has six aspirating needles 320 side by side.The fore-set of 120 bottom of incubation slot is by 24 orifice plate tops
Rise and tilt, wash sample arm group and drive aspirating needle 320 to be inserted under 24 orifice plate liquid levels, start the 4th diaphragm pump 440, six aspirating needles 320
The cleaning solution in 24 orifice plates is absorbed under the drive of the 4th diaphragm pump 440, finally the aspirating needle 320 in the case where washing the drive of sample arm group
Set back.
6. hybridization running：Hybridization journey of the nucleic acid hybridization oven 10 according to set by control mechanism 500 (such as PLC controller etc.)
Sequence, disparity items can be adjusted suitably in each step run time and reagent dosage, reach effective within the specific limits
Accuracy.A variety of reagents are added to 24 orifice plate sample wells by pipetting device 200 and pump liquid mechanism 400.
Realize the liquid feeding of cleaning solution by washing model machine structure 300 and pump liquid mechanism 400 and remove and operate；It is further by control mechanism 500
Control the temperature of incubation slot 120, it is possible to achieve preheating, hybridization.Sample is taken out after hybridization to be dyed, then is shown by fluorescence
Micro mirror, that is, observable testing result.
The nucleic acid hybridization oven 10 can realize whole full-automatic experimental implementation by setting hybridization procedures, be different from miscellaneous
The manual operation in numerous and diverse step process is handed over, avoids consuming substantial amounts of manpower and time, eliminates the error caused by human factor,
Save substantial amounts of experimental period.Using independent reagent addition, cleaning regulation device, and cleaning solution liquid feeding and absorb regulation and control dress
Put, and corresponding driving device precise motion is controlled by control mechanism, realize that speed is quick, easy to operate, accurately liquid feeding
And imbibition operation；Heated at constant temperature is controlled by control mechanism, heating directly, it is quick, and by box lid sealing ensure incubation temperature and
Reaction reagent is effectively retained, and is effectively improved experiment accuracy, reduces experimental error.
Each technical characteristic of embodiment described above can be combined arbitrarily, to make description succinct, not to above-mentioned reality
Apply all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, the scope that this specification is recorded all is considered to be.
Embodiment described above only expresses the several embodiments of the utility model, its description is more specific and detailed,
But therefore it can not be interpreted as the limitation to utility model patent scope.It should be pointed out that the common skill for this area
For art personnel, without departing from the concept of the premise utility, various modifications and improvements can be made, these are belonged to
The scope of protection of the utility model.Therefore, the protection domain of the utility model patent should be determined by the appended claims.
Claims (10)
- A kind of 1. nucleic acid hybridization oven, it is characterised in that including：Board, the board have reagent area, Xi Zhen areas, are incubated area and washing lotion area, and the reagent area is used to place reagent, described Xi Zhen areas, which have, washes pin pond, and the area that is incubated has the incubation slot for being used for placing response container, and the washing lotion area is used to place clear Washing lotion；Pipetting device, including sample needle and sample-adding driving device, it is described sample-adding driving device be located on the board and with it is described Sample needle connection is moved for the driving sample needle between the reagent area, the Xi Zhen areas and the incubation area；Wash model machine structure, including injection needle, aspirating needle and wash sample driving device, it is described wash sample driving device be located on the board and It is connected respectively with the injection needle and the aspirating needle for driving the injection needle and the aspirating needle in the Xi Zhen areas Moved between the incubation area；Pump liquid mechanism, is connected respectively with the sample needle, pin pond, the injection needle and the aspirating needle washed；AndControl mechanism, with the sample-adding driving device, described wash sample driving device and the pump liquid mechanism is connected；The control mechanism is used to control the sample-adding driving device that the sample needle is moved to the reagent area, and controls institute Pump liquid mechanism is stated via the sample needle from the reagent area pumping reagent；The control mechanism is additionally operable to control the sample-adding to drive The sample needle is moved to the incubation area by dynamic device, and controls the pump liquid mechanism to pump out the reagent in the sample needle In reaction vessel in the incubation slot；The control mechanism is additionally operable to control the sample-adding driving device by the sample needle Washed described in being moved in pin pond, and control pump liquid mechanism pumping cleaning solution from the cleaning solution in the washing lotion area clear with difference Wash the inner and outer wall of the sample needle；The control mechanism is additionally operable to control the sample-adding driving device to answer the sample needle Position；The control mechanism is additionally operable to control the pump liquid mechanism to pump out the cleaning waste liquid washed in pin pond；The control machine Structure is additionally operable to wash sample driving device described in control the injection needle is moved to the incubation area, and control the pump liquid mechanism from Pumping cleaning solution and pumped out in the cleaning solution in the washing lotion area by the injection needle into the reaction vessel；The control machine Structure is additionally operable to wash sample driving device described in control the aspirating needle is moved to the incubation area, and controls the pump liquid mechanism will Cleaning waste liquid in the reaction vessel is pumped out via the aspirating needle；The control mechanism is additionally operable to wash sample driving described in control Device by the injection needle and the aspirating needle be respectively moved to it is described wash in pin pond, and control the pump liquid mechanism to be washed from described Pumping cleaning solution is to be respectively washed the injection needle and the aspirating needle in the cleaning solution of liquid zone；The control mechanism is additionally operable to control Sample driving device is washed described in system drives the injection needle and the aspirating needle to reset.
- 2. nucleic acid hybridization oven as claimed in claim 1, it is characterised in that the reagent area has reagent rack；The reagent rack Bottom there is first heater, the first heater is connected with the control mechanism.
- 3. nucleic acid hybridization oven as claimed in claim 1, it is characterised in that the bottom of the incubation slot is equipped with the second heating and fills Put, the secondary heating mechanism is connected with the control mechanism；And/orThe bottom of the incubation slot is equipped with jacking mechanism, and the jacking mechanism is connected with by the control machine with the control mechanism The side of the reaction vessel in the incubation slot is jacked up to form inclination by structure control；And/orTemperature sensor is equipped with the incubation slot, the temperature sensor is electrically connected with the control mechanism；And/orThe top of the incubation slot is equipped with box lid.
- 4. nucleic acid hybridization oven as claimed in claim 3, it is characterised in that further include the box lid being connected with the control mechanism and drive Dynamic device, the box lid driving device are connected with the box lid for by driving the box lid to move described in open or close Incubation slot.
- 5. nucleic acid hybridization oven as claimed in claim 1, it is characterised in that the washing lotion area is equipped with multiple bottle for handling liquid toilet or cosmetic substance, Duo Gexi Liquid bottle is connected by pipeline with the pump liquid mechanism respectively；Each independent control is equipped with the corresponding pipeline of the bottle for handling liquid toilet or cosmetic substance Valve, the control valve are connected with the control mechanism to be opened or closed by control mechanism control.
- 6. nucleic acid hybridization oven as claimed in claim 1, it is characterised in that the injection needle and the aspirating needle are in a row set respectively Put, and rows of multiple injection needles are arranged at intervals with rows of multiple aspirating needles.
- 7. such as nucleic acid hybridization oven according to any one of claims 1 to 6, it is characterised in that the pump liquid mechanism includes plunger Pump and the first diaphragm pump, the plunger pump and first diaphragm pump are concatenated with the sample needle, and the plunger pump is used to control The sample needle is made to pump out the reagent of pumping to the reaction from the reagent area pumping reagent and the control sample needle In container, first diaphragm pump is used for from the washing lotion area pumping cleaning solution and is pumped into the sample needle and pumps out to institute State and wash in pin pond to clean the inner and outer wall of the sample needle.
- 8. nucleic acid hybridization oven as claimed in claim 7, it is characterised in that the pump liquid mechanism further includes the second diaphragm pump, institute State the second diaphragm pump to concatenate with the pin pond of washing, second diaphragm pump is used to pump out the waste liquid for washing pin pond.
- 9. nucleic acid hybridization oven as claimed in claim 8, it is characterised in that the pump liquid mechanism further includes the 3rd diaphragm pump and Four diaphragm pumps, the 3rd diaphragm pump are concatenated with the injection needle with from the washing lotion area pumping cleaning solution and via the fluid injection Pin pumps out, and the 4th diaphragm pump is concatenated with the aspirating needle with by the aspirating needle, pumping is cleaned out of described reaction vessel Waste liquid simultaneously pumps out.
- 10. nucleic acid hybridization oven as claimed in claim 9, it is characterised in that further include the waste liquid bottle being located on the board and connect Mouthful, the waste liquid bottle interface is connected with second diaphragm pump and the 4th diaphragm pump.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721200708.7U CN207259496U (en) | 2017-09-18 | 2017-09-18 | Nucleic acid hybridization oven |
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| CN201721200708.7U CN207259496U (en) | 2017-09-18 | 2017-09-18 | Nucleic acid hybridization oven |
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| CN207259496U true CN207259496U (en) | 2018-04-20 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107629952A (en) * | 2017-09-18 | 2018-01-26 | 益善生物技术股份有限公司 | Nucleic acid hybridization oven |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107629952A (en) * | 2017-09-18 | 2018-01-26 | 益善生物技术股份有限公司 | Nucleic acid hybridization oven |
| CN107629952B (en) * | 2017-09-18 | 2024-03-19 | 益善生物技术股份有限公司 | Nucleic acid hybridization instrument |
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