CN114317242A - Large-volume cell liquid concentration system and method - Google Patents
Large-volume cell liquid concentration system and method Download PDFInfo
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- CN114317242A CN114317242A CN202111657993.6A CN202111657993A CN114317242A CN 114317242 A CN114317242 A CN 114317242A CN 202111657993 A CN202111657993 A CN 202111657993A CN 114317242 A CN114317242 A CN 114317242A
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- 238000000034 method Methods 0.000 title claims abstract description 21
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 70
- 230000001413 cellular effect Effects 0.000 claims abstract description 43
- 239000012530 fluid Substances 0.000 claims abstract description 42
- 210000004027 cell Anatomy 0.000 claims description 81
- 239000002699 waste material Substances 0.000 claims description 34
- 238000012544 monitoring process Methods 0.000 claims description 8
- 230000002093 peripheral effect Effects 0.000 claims description 8
- 238000000926 separation method Methods 0.000 claims description 5
- 238000009987 spinning Methods 0.000 claims description 4
- 210000000172 cytosol Anatomy 0.000 claims 1
- 238000011282 treatment Methods 0.000 abstract description 4
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
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- 238000012545 processing Methods 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
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- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- 238000004113 cell culture Methods 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
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- 238000012258 culturing Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
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- 238000002360 preparation method Methods 0.000 description 1
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Abstract
The invention provides a large-volume cellular liquid concentration system, which comprises a control module, an external control component and a large-volume cellular liquid concentration device, wherein the external control component is arranged on the control module; the external control part comprises a rotary pressure valve, a plurality of peristaltic pumps and a centrifugal mechanism; the rotary pressure valve, the peristaltic pumps and the centrifugal mechanism are arranged in the large-volume cellular fluid concentrating device; the control module controls the rotary pressure valve, the peristaltic pumps and the centrifugal mechanism so as to actuate the cell sap to be quickly concentrated in the large-volume cell sap concentrating device; the liquid inlet part of the large-volume cellular liquid concentrating device is provided with a plurality of liquid path branches. The invention also provides a method for concentrating large-volume cellular fluid. According to the large-volume cellular fluid concentration system and method provided by the invention, the liquid inlet amount during cellular fluid treatment is increased by arranging the plurality of liquid path branches, so that the cellular fluid with large-volume liquid amount can be rapidly concentrated, the cellular fluid concentration efficiency is improved, and the cell collection efficiency is also improved.
Description
Technical Field
The invention belongs to the field of cell processing, and particularly relates to a large-volume cellular fluid concentration system and a large-volume cellular fluid concentration method.
Background
In the process of culturing biological cells, a culture medium containing cell growth factors and nutrients, oxygen, air, carbon dioxide and the like are continuously added to the cells to be cultured to promote the growth of the biological cells. When the cell culture is completed, the cultured cell fluid needs to be subjected to treatments such as "concentration" and "washing" to remove excess medium, metabolic waste liquid, and the like from the cell fluid. Under different culture requirements and conditions, the cultured cell sap has large volume difference, and the volume interval difference even reaches from 200ml to 50L.
For the concentration of cell sap, there have been automated apparatus processes in the prior art, but the recovery rate of cells after the above-mentioned automated apparatus concentration process is low, and the efficiency of concentration washing is low when the cell sap volume is large. In addition to the above-mentioned methods, when the volume of the cellular fluid to be treated is large, the operator may use the manual treatment method, but the manual treatment method is complicated, time-consuming and labor-consuming, results are not consistent well, recovery efficiency differences are large, and contamination may occur.
Disclosure of Invention
In order to solve at least one of the above technical problems, the present invention provides a system and a method for concentrating a large volume of cellular fluid, which can achieve all operations such as concentration, preparation, and split charging in a closed environment.
In order to at least achieve one of the above purposes, the invention adopts the technical scheme that:
the invention provides a large-volume cellular liquid concentration system, which comprises a control module, an external control component and a large-volume cellular liquid concentration device, wherein the external control component is arranged on the control module; the external control part comprises a rotary pressure valve, a plurality of peristaltic pumps and a centrifugal mechanism; the rotary pressure valve, the peristaltic pumps and the centrifugal mechanism are arranged in the large-volume cellular fluid concentrating device; the control module controls the rotary pressure valve, the peristaltic pumps and the centrifugal mechanism so as to actuate the cell sap to be quickly concentrated in the large-volume cell sap concentrating device; the liquid inlet part of the large-volume cellular liquid concentrating device is provided with a plurality of liquid path branches.
Further, the control module comprises a controller, and the rotary pressure valve, the plurality of peristaltic pumps and the centrifugal mechanism are all electrically connected with the controller.
Further, the large-volume cellular liquid concentrating device comprises a sample pipeline, a liquid inlet pipeline and a collecting pipeline; at least two parallel sample liquid path branches are arranged between the sample pipeline and the liquid inlet pipeline; at least two parallel return liquid path branches are arranged between the collecting pipeline and the liquid inlet pipeline; the cell sap passes through the sample pipeline, the sample liquid pipeline branch and the liquid inlet pipeline and then is concentrated; and subpackaging the concentrated cell sap through the liquid inlet pipeline, the return liquid pipeline branch and the collecting pipeline.
Further, the two parallel sample liquid path branches are: one sample liquid path branch is that the sample liquid path is communicated with the liquid inlet path through a first three-way joint, a first pump pipe path and a second three-way joint; and the other sample liquid path branch is that the sample liquid path is communicated with the liquid inlet path through a first three-way joint, a connecting path, a third three-way joint, a second pump pipe path and a second three-way joint.
Furthermore, a first peristaltic pump is arranged on the second pump pipeline, and a second peristaltic pump is arranged on the first pump pipeline; the liquid inlet pipe is directly connected with the liquid outlet pipe.
Further, the two parallel return fluid path branches are: a return fluid path branch is communicated with the collection pipeline through a second three-way joint, a first pump pipeline, a first three-way joint, a connecting pipeline and a third three-way joint of the fluid inlet pipeline; and the other return liquid path branch is formed by communicating the liquid inlet pipeline with the collecting pipeline through a second three-way joint, a second pump pipeline and a third three-way joint.
Further, the sample pipeline and the product bag pipeline are arranged in the spinning valve, and the spinning valve controls the connection and disconnection of the sample pipeline and the product bag pipeline; the separation cup is further connected with a waste liquid pipeline, and the waste liquid pipeline is respectively communicated with the second pressure monitoring pipeline and the waste liquid bag pipeline through a fifth three-way joint.
Furthermore, the first three-way joint, the second three-way joint, the third three-way joint and the fourth three-way joint are T-shaped three-way joints; the fifth three-way joint is a Y-shaped three-way joint; the tail end of the sample pipeline is provided with a first puncture needle; and a second puncture needle is arranged at the tail end of the waste liquid bag pipeline.
Further, the peripheral control part further comprises an air detector, and the air detector is arranged on the liquid inlet pipeline.
The present invention also provides a method according to the above-described bulk cellular liquid concentration system,
the first step is as follows: communicating a cell fluid sample bag with a sample pipeline through a first puncture needle, and communicating a waste fluid bag with a waste fluid bag pipeline through a second puncture needle; the rotary pressure valve rotates to open the sample pipeline and close the product bag pipeline; the first peristaltic pump and the second peristaltic pump rotate clockwise, so that cell sap enters the two sample branches simultaneously through the first three-way joint; wherein, the sample branch is a branch from cell sap to a second three-way joint through a connecting pipeline, a third three-way joint and a second pump pipeline; the other sample branch is cell fluid and reaches the second three-way joint through the first pump pipeline; cell sap in the two sample branches are mixed at the second tee joint and then enter a liquid inlet pipeline, and the air detector detects the cell sap and transmits signals to the controller; cell sap enters the centrifuge cup through the liquid inlet pipeline, the centrifuge cup is driven by the centrifuge to rotate at a high speed, the cell sap is separated in the centrifuge cup, and the discharged waste liquid enters the waste liquid bag through the waste liquid pipeline, the fifth joint and the waste liquid bag pipeline; when all the cell sap in the cell sap sample bag enters the centrifugal cup, the air detector detects the air in the liquid inlet pipeline again, the first peristaltic pump and the second peristaltic pump stop running at the moment, and the centrifugal machine stops rotating;
the second step is that: after the cell sap is concentrated, the rotary pressure valve rotates, the sample pipeline is closed, and the product bag pipeline is opened; the first peristaltic pump and the second peristaltic pump rotate anticlockwise, and cell sap in the centrifugal cup reaches the second three-way joint through the liquid inlet pipeline and then simultaneously enters the two return branches through the second three-way joint; wherein, a return branch is used for leading the cell sap to reach the third tee joint through the second pump pipeline; the other return branch is that the cell sap reaches a third tee joint through a first pump pipe, a first tee joint and a connecting pipe; cell sap in the two return branches is converged at a third tee joint and then enters a collecting pipeline, and then enters a product bag through a fourth tee joint and a product bag pipeline to be collected after concentration;
the third step: calculating the residual cell sap amount in the centrifugal cup according to the rotation turns of the first peristaltic pump and the second peristaltic pump; when the residual amount reaches a preset value, stopping the rotation of the first peristaltic pump and the second peristaltic pump, starting the centrifugal machine, clockwise rotating for a preset first time, and anticlockwise rotating for a preset second time; after the circulation is carried out for a plurality of times, the centrifugal machine stops again; the first peristaltic pump and the second peristaltic pump continue to rotate anticlockwise, and the residual cell sap in the centrifuge cup is pumped into the product bag;
the fourth step: when the air detector finds air again, the first peristaltic pump and the second peristaltic pump stop after rotating for a certain number of turns, and the collection of concentrated cell sap is completed.
Compared with the prior art, the large-volume cell liquid concentration system and the method provided by the invention have the beneficial effects that:
the invention provides a large-volume cellular fluid concentration system and a method, wherein a rotary pressure valve, a plurality of peristaltic pumps and a centrifugal mechanism in an external control part are controlled by a control module; and carry out reasonable connection through setting up many pipelines and a plurality of three way connection to establish two liquid route branches, the feed liquor volume when having increased cell sap processing, thereby can the cell sap that the rapid concentration has bulky liquid measure, improved the concentrated efficiency of cell sap, also improved the efficiency that the cell was collected simultaneously.
Drawings
FIG. 1 is a schematic diagram of a large volume cellular liquid concentration system provided by the present invention;
FIG. 2 is a schematic diagram of a large volume cellular fluid concentration system according to the present invention;
the system comprises a first puncture needle 1, a sample pipeline 2, a first three-way joint 3, a first pump pipeline 4, a connecting pipeline 5, a second pump pipeline 6, a second three-way joint 7, a liquid inlet pipeline 8, a third three-way joint 9, a collecting pipeline 10, a fourth three-way joint 11, a first pressure monitoring pipeline 12, a product bag pipeline 14, a product bag 15, a separating cup 16, a waste liquid pipeline 17, a fifth three-way joint 18, a second pressure monitoring pipeline 19, a waste liquid bag pipeline 20, a second puncture needle 21, an air detector 22, a first peristaltic pump 23, a second peristaltic pump 24, a rotary pressure valve 25 and a centrifugal machine 26.
Detailed Description
In order to make the technical solutions of the present invention better understood by those skilled in the art, the present invention will be further described in detail with reference to specific examples. Note that the following described embodiments are illustrative only for explaining the present invention, and are not to be construed as limiting the present invention. The examples, where specific techniques or conditions are not indicated, are to be construed according to the techniques or conditions described in the literature in the art or according to the product specifications.
In the description of the present invention, it is to be understood that the terms "first", "second" and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implying any number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; the connection can be mechanical connection or electrical connection; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
Hereinafter, the large-volume cellular fluid concentration system and method provided by the present invention will be described in detail by specific examples:
as shown in fig. 1, the large-volume cellular liquid concentrating system provided by the present invention mainly comprises a control module, an external control component and a large-volume cellular liquid concentrating device. The peripheral control unit is installed in the large-volume cell concentration device. The control module is electrically connected with the peripheral control component, controls the peripheral control component and actuates the large-volume cell concentration device through the peripheral control component. The peripheral control component also feeds back the detected state information of the large-volume cell concentration device to the control system.
The control module includes a Controller (CPU). The peripheral control components include an air detector 22, a first peristaltic pump 23, a second peristaltic pump 24, a rotary pressure valve 25, and a centrifuge 26. The first peristaltic pump 23, the second peristaltic pump 24, the rotary pressure valve 25 and the centrifuge 26 are all electrically connected to the controller. The controller sends instructions to operate and actuate the first peristaltic pump 23, the second peristaltic pump 24, the rotary pressure valve 25 and the centrifuge 26, and further control the flow direction of cell liquid in the large-volume cell concentration device, so that the concentration and collection operation processes are completed. The air detector 22 is in communication connection with the controller, and the air detector 22 collects air signals of a pipeline in a large-volume cell liquid concentration system and transmits the air signals to the controller.
The invention provides a large-volume cellular liquid concentration device which mainly comprises parts such as a sample pipeline 2, a liquid inlet pipeline 8, a collection pipeline 10, a product bag 15, a separation cup 16, a waste liquid pipeline 17 and the like.
The tail end of the sample pipeline 2 is provided with a first puncture needle 1, and the other end of the sample pipeline 2 is respectively communicated with a first pump pipeline 4 and a connecting pipeline 5 through a first three-way joint 3. The first pump pipeline 4 is respectively communicated with a second pump pipeline 6 and a liquid inlet pipeline 8 through a second tee joint 7; the connecting pipeline 5 is respectively communicated with the second pump pipeline 6 and the collecting pipeline 10 through a third tee joint 9.
The second pump pipe 6 is provided with a first peristaltic pump 23, and the first pump pipe 4 is provided with a second peristaltic pump 24. The first peristaltic pump 23 and the second peristaltic pump 24 transfer the cellular fluid in the large-volume cellular fluid concentration device.
The collecting pipeline 10 is respectively communicated with a first pressure monitoring pipeline 12 and a product bag pipeline 14 through a fourth tee joint 11; the tail end of the first pressure monitoring pipeline 12 is provided with a pressure sensor, and the pressure sensor is used for detecting pipeline pressure; the end of the product bag conduit 14 is provided with a product bag 15.
The sample line 2 and the product bag line 14 are installed in a rotary pressure valve 25. And the rotary pressure valve 25 respectively controls the on-off of the sample pipeline and the product bag pipeline.
The separation cup 16 is arranged between the inlet line 8 and the waste line 17. The waste liquid pipeline 17 is respectively communicated with a second pressure monitoring pipeline 19 and a waste liquid bag pipeline 20 through a fifth three-way joint 18; the tail end of the second pressure monitoring pipeline 19 is provided with a pressure sensor; the end of the waste liquid bag pipeline 20 is provided with a second puncture needle 21.
An air detector 22 is arranged on the liquid inlet pipeline 8; the air detector is used for detecting an air signal in the liquid inlet pipeline. The separation cup 16 is connected to a centrifuge 26, and is rotated by the activation of the centrifuge mechanism 26 to centrifugally separate cellular fluids introduced into the interior thereof.
The invention provides a method for quickly concentrating a large-volume cellular fluid concentration system, which comprises the following steps:
the first step is as follows: a cell fluid sample bag is communicated with a sample pipeline 2 through a first puncture needle 1, and a waste fluid bag is communicated with a waste fluid bag pipeline 20 through a second puncture needle 21; the rotary pressure valve 25 rotates to open the sample pipeline 2 and close the product bag pipeline 14; the first peristaltic pump 23 and the second peristaltic pump 24 rotate clockwise, so that cell sap enters the two sample branches simultaneously through the first three-way joint 3; wherein, a sample branch is a branch from cell sap to a second three-way joint 7 through a connecting pipeline 5, a third three-way joint 9 and a second pump pipeline 6; the other sample branch is cell fluid, and then reaches the second three-way joint 7 through the first pump pipeline 4.
Cell fluids in the two sample branches are mixed at the second three-way joint 7 and then enter the fluid inlet pipeline 8, and at the moment, the air detector 22 detects the fluid and transmits signals to the controller; cell sap enters the centrifuge cup 16 through the liquid inlet pipeline 8, the centrifuge cup 16 is driven by the centrifuge 26 to rotate at a high speed, the cell sap is separated in the centrifuge cup 16, and the discharged waste liquid enters the waste liquid bag through the waste liquid pipeline 17, the fifth joint 18 and the waste liquid bag pipeline 20; when all the cell sap in the cell sap sample bag enters the centrifuge cup 16, the air detector 22 again detects the air in the liquid inlet pipeline 8, at this time, the first peristaltic pump 23 and the second peristaltic pump 24 stop running, and the centrifuge 26 stops rotating.
The second step is that: after the cell sap is concentrated, the rotary pressure valve 25 rotates, the sample pipeline 2 is closed, and the product bag pipeline 14 is opened; the first peristaltic pump 23 and the second peristaltic pump 24 rotate anticlockwise, cell liquid in the centrifugal cup 16 reaches the second three-way joint 7 through the liquid inlet pipeline 8, and then enters the two return branches through the second three-way joint 7; wherein, a return branch is the cell sap which reaches the third three-way joint 9 through the second pump pipeline 6; the other return branch is a cell sap which passes through the first pump pipe line 4, the first three-way joint 3 and the connecting pipe line 5 to reach the third three-way joint 9.
Cell sap in the two return branches is converged at the third tee joint 9 and then enters a collecting pipeline 10, and then enters a product bag 15 through a fourth tee joint 11 and a product bag pipeline 14 to be collected after concentration.
The third step: calculating the residual cell sap amount in the centrifugal cup according to the rotation number of the first peristaltic pump 23 and the second peristaltic pump 24; when the residual amount is 110mL, stopping rotating the first peristaltic pump 23 and the second peristaltic pump 24, starting the centrifuge 26, rotating clockwise for 5S, and rotating counterclockwise for 5S; after 30 cycles, centrifuge 26 is stopped again. The first peristaltic pump 23 and the second peristaltic pump 24 continue to rotate counterclockwise, pumping the remaining cell sap in the centrifuge cup 26 into the product bag 15;
the fourth step: when the air detector 22 detects air again, the first peristaltic pump 23 and the second peristaltic pump 24 are stopped after rotating for a certain number of turns, and the collection of the concentrated cell sap is completed.
In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.
Claims (10)
1. The large-volume cellular liquid concentration system is characterized by comprising a control module, an external control component and a large-volume cellular liquid concentration device; the external control part comprises a rotary pressure valve, a plurality of peristaltic pumps and a centrifugal mechanism; the rotary pressure valve, the peristaltic pumps and the centrifugal mechanism are arranged in the large-volume cellular fluid concentrating device; the control module controls the rotary pressure valve, the peristaltic pumps and the centrifugal mechanism so as to actuate the cell sap to be quickly concentrated in the large-volume cell sap concentrating device; the liquid inlet part of the large-volume cellular liquid concentrating device is provided with a plurality of liquid path branches.
2. The large volume cellular fluid concentration system of claim 1, wherein the control module comprises a controller, and the rotary pressure valve, the plurality of peristaltic pumps, and the centrifugal mechanism are all electrically connected to the controller.
3. The bulk cellular liquid concentration system according to claim 2, wherein the bulk cellular liquid concentration device comprises a sample line, a liquid inlet line, and a collection line; at least two parallel sample liquid path branches are arranged between the sample pipeline and the liquid inlet pipeline; at least two parallel return liquid path branches are arranged between the collecting pipeline and the liquid inlet pipeline; the cell sap passes through the sample pipeline, the sample liquid pipeline branch and the liquid inlet pipeline and then is concentrated; and subpackaging the concentrated cell sap through the liquid inlet pipeline, the return liquid pipeline branch and the collecting pipeline.
4. The large volume cellular liquid concentration system of claim 3, wherein the two parallel sample liquid path branches are: one sample liquid path branch is that the sample liquid path is communicated with the liquid inlet path through a first three-way joint, a first pump pipe path and a second three-way joint; and the other sample liquid path branch is that the sample liquid path is communicated with the liquid inlet path through a first three-way joint, a connecting path, a third three-way joint, a second pump pipe path and a second three-way joint.
5. The large volume cellular liquid concentrating system according to claim 4, wherein a first peristaltic pump is disposed on the second pump line, and a second peristaltic pump is disposed on the first pump line; the liquid inlet pipe is directly connected with the liquid outlet pipe.
6. The large volume cellular liquid concentrating system of claim 5, wherein the two parallel return path branches are: a return fluid path branch is communicated with the collection pipeline through a second three-way joint, a first pump pipeline, a first three-way joint, a connecting pipeline and a third three-way joint of the fluid inlet pipeline; and the other return liquid path branch is formed by communicating the liquid inlet pipeline with the collecting pipeline through a second three-way joint, a second pump pipeline and a third three-way joint.
7. The large volume cellular liquid concentrating system according to claim 6, wherein the sample line and the product bag line are installed in the spinning valve, the spinning valve controlling the opening and closing of the sample line and the product bag line; the separation cup is further connected with a waste liquid pipeline, and the waste liquid pipeline is respectively communicated with the second pressure monitoring pipeline and the waste liquid bag pipeline through a fifth three-way joint.
8. The large volume cytosol concentration system as recited in claim 7, wherein said first tee joint, second tee joint, third tee joint and fourth tee joint are T-shaped tee joints; the fifth three-way joint is a Y-shaped three-way joint; the tail end of the sample pipeline is provided with a first puncture needle; and a second puncture needle is arranged at the tail end of the waste liquid bag pipeline.
9. The large volume cellular liquid concentrating system according to claim 8, wherein the peripheral control unit further comprises an air detector disposed on the liquid inlet line.
10. The method for a large volume cellular liquid concentrating system according to any one of claims 1 to 9,
the first step is as follows: communicating a cell fluid sample bag with a sample pipeline through a first puncture needle, and communicating a waste fluid bag with a waste fluid bag pipeline through a second puncture needle; the rotary pressure valve rotates to open the sample pipeline and close the product bag pipeline; the first peristaltic pump and the second peristaltic pump rotate clockwise, so that cell sap enters the two sample branches simultaneously through the first three-way joint; wherein, the sample branch is a branch from cell sap to a second three-way joint through a connecting pipeline, a third three-way joint and a second pump pipeline; the other sample branch is cell fluid and reaches the second three-way joint through the first pump pipeline; cell sap in the two sample branches are mixed at the second tee joint and then enter a liquid inlet pipeline, and the air detector detects the cell sap and transmits signals to the controller; cell sap enters the centrifuge cup through the liquid inlet pipeline, the centrifuge cup is driven by the centrifuge to rotate at a high speed, the cell sap is separated in the centrifuge cup, and the discharged waste liquid enters the waste liquid bag through the waste liquid pipeline, the fifth joint and the waste liquid bag pipeline; when all the cell sap in the cell sap sample bag enters the centrifugal cup, the air detector detects the air in the liquid inlet pipeline again, the first peristaltic pump and the second peristaltic pump stop running at the moment, and the centrifugal machine stops rotating;
the second step is that: after the cell sap is concentrated, the rotary pressure valve rotates, the sample pipeline is closed, and the product bag pipeline is opened; the first peristaltic pump and the second peristaltic pump rotate anticlockwise, and cell sap in the centrifugal cup reaches the second three-way joint through the liquid inlet pipeline and then simultaneously enters the two return branches through the second three-way joint; wherein, a return branch is used for leading the cell sap to reach the third tee joint through the second pump pipeline; the other return branch is that the cell sap reaches a third tee joint through a first pump pipe, a first tee joint and a connecting pipe; cell sap in the two return branches is converged at a third tee joint and then enters a collecting pipeline, and then enters a product bag through a fourth tee joint and a product bag pipeline to be collected after concentration;
the third step: calculating the residual cell sap amount in the centrifugal cup according to the rotation turns of the first peristaltic pump and the second peristaltic pump; when the residual amount reaches a preset value, stopping the rotation of the first peristaltic pump and the second peristaltic pump, starting the centrifugal machine, clockwise rotating for a preset first time, and anticlockwise rotating for a preset second time; after the circulation is carried out for a plurality of times, the centrifugal machine stops again; the first peristaltic pump and the second peristaltic pump continue to rotate anticlockwise, and the residual cell sap in the centrifuge cup is pumped into the product bag;
the fourth step: when the air detector finds air again, the first peristaltic pump and the second peristaltic pump stop after rotating for a certain number of turns, and the collection of concentrated cell sap is completed.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115123985A (en) * | 2022-07-12 | 2022-09-30 | 深圳赛桥生物创新技术有限公司 | Pipeline emptying method and device |
| CN116080962A (en) * | 2022-12-15 | 2023-05-09 | 中博瑞康(上海)生物技术有限公司 | Large-volume cell replacement and split charging method and device |
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