CN216550374U - Large-volume cellular fluid concentration device - Google Patents
Large-volume cellular fluid concentration device Download PDFInfo
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- CN216550374U CN216550374U CN202123303678.1U CN202123303678U CN216550374U CN 216550374 U CN216550374 U CN 216550374U CN 202123303678 U CN202123303678 U CN 202123303678U CN 216550374 U CN216550374 U CN 216550374U
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Abstract
The utility model provides a large-volume cellular fluid concentration device, which comprises a sample pipeline, a fluid inlet pipeline and a collection pipeline; at least two parallel sample liquid path branches are arranged between the sample pipeline and the liquid inlet pipeline; at least two parallel return liquid path branches are arranged between the collecting pipeline and the liquid inlet pipeline; the cell sap passes through the sample pipeline, the sample liquid pipeline branch and the liquid inlet pipeline and then is concentrated; and subpackaging the concentrated cell sap through the liquid inlet pipeline, the return liquid pipeline branch and the collecting pipeline. According to the large-volume cellular fluid concentration device provided by the utility model, the plurality of pipelines and the plurality of tee joints are reasonably connected, so that two fluid path branches are established, the liquid inlet amount during cellular fluid treatment is increased, the cellular fluid with large-volume liquid amount can be rapidly concentrated, the cellular fluid concentration efficiency is improved, and the cell collection efficiency is also improved.
Description
Technical Field
The utility model belongs to the field of biological cell treatment, and particularly relates to a large-volume cellular fluid concentration device.
Background
In the process of culturing biological cells, a culture medium containing cell growth factors and nutrients, oxygen, air, carbon dioxide and the like are continuously added to the cells to be cultured to promote the growth of the biological cells. When the cell culture is completed, the cultured cell fluid needs to be subjected to treatments such as "concentration" and "washing" to remove excess medium, metabolic waste liquid, and the like from the cell fluid. Under different culture requirements and conditions, the cultured cell sap has large volume difference, and the volume interval difference even reaches from 200ml to 50L.
For the concentration of cell sap, there have been automated apparatus processes in the prior art, but the recovery rate of cells after the above-mentioned automated apparatus concentration process is low, and the efficiency of concentration washing is low when the cell sap volume is large. In addition to the above-mentioned methods, when the volume of the cellular fluid to be treated is large, the operator may use the manual treatment method, but the manual treatment method is complicated, time-consuming and labor-consuming, results are not consistent well, recovery efficiency differences are large, and contamination may occur.
SUMMERY OF THE UTILITY MODEL
In order to solve at least one of the above technical problems, the present invention provides a large-volume cellular fluid concentrating device, which can rapidly concentrate cellular fluid with large volume of fluid, thereby improving the efficiency of cellular fluid concentration and the efficiency of cell collection.
In order to at least achieve one of the above purposes, the utility model adopts the technical scheme that:
the utility model provides a large-volume cellular fluid concentration device, which comprises a sample pipeline, a fluid inlet pipeline and a collection pipeline, wherein the sample pipeline is connected with the fluid inlet pipeline; at least two parallel sample liquid path branches are arranged between the sample pipeline and the liquid inlet pipeline; at least two parallel return liquid path branches are arranged between the collecting pipeline and the liquid inlet pipeline; the cell sap passes through the sample pipeline, the sample liquid pipeline branch and the liquid inlet pipeline and then is concentrated; and collecting the concentrated cell sap through the liquid inlet pipeline, the return liquid branch and the collecting pipeline.
Further, the two parallel sample liquid path branches are: one sample liquid path branch is that the sample liquid path is communicated with the liquid inlet path through a first three-way joint, a first pump pipe path and a second three-way joint; and the other sample liquid path branch is that the sample liquid path is communicated with the liquid inlet path through a first three-way joint, a connecting path, a third three-way joint, a second pump pipe path and a second three-way joint.
Further, the two parallel return fluid path branches are: a return fluid path branch is communicated with the collection pipeline through a second three-way joint, a first pump pipeline, a first three-way joint, a connecting pipeline and a third three-way joint of the fluid inlet pipeline; and the other return liquid path branch is formed by communicating the liquid inlet pipeline with the collecting pipeline through a second three-way joint, a second pump pipeline and a third three-way joint.
Further, the device also comprises a separation cup, and the separation cup is directly connected with the liquid inlet pipeline.
Furthermore, the other end of the collecting pipeline is respectively communicated with the first pressure monitoring pipeline and the product bag pipeline through a fourth tee joint.
Further, the product bag is directly connected with the product bag pipeline.
Furthermore, the separating cup is also connected with a waste liquid pipeline, and the waste liquid pipeline is respectively communicated with the second pressure monitoring pipeline and the waste liquid bag pipeline through a fifth three-way joint.
Further, the tail ends of the first pressure monitoring pipeline and the second pressure monitoring pipeline are provided with pressure sensors, and the pressure sensors are used for detecting pipeline pressure.
Furthermore, the first three-way joint, the second three-way joint, the third three-way joint and the fourth three-way joint are T-shaped three-way joints; and the fifth three-way joint is a Y-shaped three-way joint.
Further, a first puncture needle is arranged at the tail end of the sample pipeline; and a second puncture needle is arranged at the tail end of the waste liquid bag pipeline.
Compared with the prior art, the large-volume cellular fluid concentration device provided by the utility model has the beneficial effects that:
according to the large-volume cellular fluid concentration device provided by the utility model, the plurality of pipelines and the plurality of tee joints are reasonably connected, so that two fluid path branches are established, the liquid inlet amount during cellular fluid treatment is increased, the cellular fluid with large-volume liquid amount can be rapidly concentrated, the cellular fluid concentration efficiency is improved, and the cell collection efficiency is also improved.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the scope of the application.
Drawings
FIG. 1 is a schematic structural diagram of a large-volume cellular liquid concentration device in an embodiment of the present application;
the system comprises a first puncture needle 1, a sample pipeline 2, a first three-way joint 3, a first pump pipeline 4, a connecting pipeline 5, a second pump pipeline 6, a second three-way joint 7, a liquid inlet pipeline 8, a third three-way joint 9, a collecting pipeline 10, a fourth three-way joint 11, a first pressure monitoring pipeline 12, a pressure sensor 13, a product bag pipeline 14, a product bag 15, a separation cup 16, a waste liquid pipeline 17, a fifth three-way joint 18, a second pressure monitoring pipeline 19, a waste liquid bag pipeline 20 and a second puncture needle 21.
Detailed Description
In order to make the technical solutions of the present invention better understood by those skilled in the art, the present invention will be further described in detail with reference to specific examples. Note that the following described embodiments are illustrative only for explaining the present invention, and are not to be construed as limiting the present invention. The examples, where specific techniques or conditions are not indicated, are to be construed according to the techniques or conditions described in the literature in the art or according to the product specifications.
In the description of the present invention, it is to be understood that the terms "first", "second" and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implying any number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; the connection can be mechanical connection or electrical connection; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
Hereinafter, the large-volume cellular liquid concentrating apparatus provided by the present invention will be described in detail by specific examples:
as shown in fig. 1, the present embodiment provides a large-volume cellular liquid concentrating apparatus, which mainly includes components such as a sample pipeline 2, a liquid inlet pipeline 8, a collection pipeline 10, a product bag 15, a separation cup 16, and a waste liquid pipeline 17.
The tail end of the sample pipeline 2 is provided with a first puncture needle 1, and the other end of the sample pipeline 2 is respectively communicated with a first pump pipeline 4 and a connecting pipeline 5 through a first three-way joint 3. The first pump pipeline 4 is respectively communicated with a second pump pipeline 6 and a liquid inlet pipeline 8 through a second tee joint 7; the connecting pipeline 5 is respectively communicated with the second pump pipeline 6 and the collecting pipeline 10 through a third tee joint 9.
The collecting pipeline 10 is respectively communicated with a first pressure monitoring pipeline 12 and a product bag pipeline 14 through a fourth tee joint 11; the tail end of the first pressure monitoring pipeline 12 is provided with a pressure sensor 13, and the pressure sensor 13 is used for detecting pipeline pressure; the end of the product bag conduit 14 is provided with a product bag 15.
The separation cup 16 is arranged between the inlet line 8 and the waste line 17. The waste liquid pipeline 17 is respectively communicated with a second pressure monitoring pipeline 19 and a waste liquid bag pipeline 20 through a fifth three-way joint 18; the tail end of the second pressure monitoring pipeline 19 is provided with a pressure sensor; the end of the waste liquid bag pipeline 20 is provided with a second puncture needle 21.
The specific working principle of the large-volume cellular liquid concentrating device provided by the embodiment is described as follows:
the cell fluid sample bag is communicated with the sample pipeline 2 through the first puncture needle 1, and the waste fluid bag is communicated with the waste fluid bag pipeline 20 through the second puncture needle 21. Cell fluid is driven by a power device (not shown in the figure) to simultaneously enter the two sample branches through the first three-way joint 3; wherein, a sample branch is a branch from cell sap to a second three-way joint 7 through a connecting pipeline 5, a third three-way joint 9 and a second pump pipeline 6; the other sample branch is cell fluid, and then reaches the second three-way joint 7 through the first pump pipeline 4.
Cell sap in the two sample branches are mixed at the second three-way joint 7 and then enter the liquid inlet pipeline 8, and enter the centrifugal cup 16 through the liquid inlet pipeline 8. The cell liquid is separated in the centrifuge cup 16, and the waste liquid discharged by centrifugation enters the waste liquid bag through the waste liquid pipeline 17, the fifth three-way joint 18 and the waste liquid bag pipeline 20. The cell sap concentrated by centrifugation reaches the second three-way joint 7 through the liquid inlet pipeline 8 under the driving of the power device, and then enters two return branches through the second three-way joint 7; wherein, a return branch is the cell sap which reaches the third three-way joint 9 through the second pump pipeline 6; the other return branch is a cell sap which passes through the first pump pipe line 4, the first three-way joint 3 and the connecting pipe line 5 to reach the third three-way joint 9.
Cell sap in the two return branches is converged at the third tee joint 9 and then enters a collecting pipeline 10, and then enters a product bag 15 through a fourth tee joint 11 and a product bag pipeline 14, so that the cell sap is collected after concentration.
Preferably, in the large-volume cellular liquid concentrating device provided by this embodiment, the first three-way joint 3, the second three-way joint 7, the third three-way joint 9, and the fourth three-way joint 11 are T-shaped three-way joints. The fifth three-way joint 18 is a Y-shaped three-way joint.
In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the utility model. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.
Claims (10)
1. A large-volume cellular fluid concentration device is characterized by comprising a sample pipeline, a fluid inlet pipeline and a collection pipeline; at least two parallel sample liquid path branches are arranged between the sample pipeline and the liquid inlet pipeline; at least two parallel return liquid path branches are arranged between the collecting pipeline and the liquid inlet pipeline; the cell sap passes through the sample pipeline, the sample liquid pipeline branch and the liquid inlet pipeline and then is concentrated; and collecting the concentrated cell sap through the liquid inlet pipeline, the return liquid branch and the collecting pipeline.
2. The device of claim 1, wherein the two parallel sample fluid path branches are: one sample liquid path branch is that the sample liquid path is communicated with the liquid inlet path through a first three-way joint, a first pump pipe path and a second three-way joint; and the other sample liquid path branch is that the sample liquid path is communicated with the liquid inlet path through a first three-way joint, a connecting path, a third three-way joint, a second pump pipe path and a second three-way joint.
3. The large volume cellular liquid concentrating device according to claim 2, wherein the two parallel return liquid path branches are: a return fluid path branch is communicated with the collection pipeline through a second three-way joint, a first pump pipeline, a first three-way joint, a connecting pipeline and a third three-way joint of the fluid inlet pipeline; and the other return liquid path branch is formed by communicating the liquid inlet pipeline with the collecting pipeline through a second three-way joint, a second pump pipeline and a third three-way joint.
4. The large-volume cellular liquid concentrating device according to claim 3, further comprising a separation cup directly connected to the liquid inlet line.
5. The large-volume cellular liquid concentrating device according to claim 4, wherein the other end of the collecting line is communicated with the first pressure monitoring line and the product bag line through a fourth three-way joint, respectively.
6. The large volume cellular liquid concentrating device according to claim 5, further comprising a product bag directly connected to the product bag conduit.
7. The large-volume cellular liquid concentrating device according to claim 6, wherein the separating cup is further connected to a waste liquid line, and the waste liquid line is respectively communicated with the second pressure monitoring line and the waste liquid bag line through a fifth three-way joint.
8. The large-volume cellular liquid concentrating device according to claim 7, wherein the first pressure monitoring line and the second pressure monitoring line are each provided at an end thereof with a pressure sensor for detecting a line pressure.
9. The large volume cytosol concentrating device of claim 8, wherein said first tee joint, second tee joint, third tee joint and fourth tee joint are T-shaped tee joints; and the fifth three-way joint is a Y-shaped three-way joint.
10. The large-volume cellular liquid concentrating device according to claim 9, wherein a first puncture needle is provided at a distal end of the sample line; and a second puncture needle is arranged at the tail end of the waste liquid bag pipeline.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123303678.1U CN216550374U (en) | 2021-12-24 | 2021-12-24 | Large-volume cellular fluid concentration device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123303678.1U CN216550374U (en) | 2021-12-24 | 2021-12-24 | Large-volume cellular fluid concentration device |
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| Publication Number | Publication Date |
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| CN216550374U true CN216550374U (en) | 2022-05-17 |
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| CN202123303678.1U Active CN216550374U (en) | 2021-12-24 | 2021-12-24 | Large-volume cellular fluid concentration device |
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2021
- 2021-12-24 CN CN202123303678.1U patent/CN216550374U/en active Active
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