CN217786697U - Concentrated washing device of bulky cellular liquid - Google Patents
Concentrated washing device of bulky cellular liquid Download PDFInfo
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- CN217786697U CN217786697U CN202220111922.XU CN202220111922U CN217786697U CN 217786697 U CN217786697 U CN 217786697U CN 202220111922 U CN202220111922 U CN 202220111922U CN 217786697 U CN217786697 U CN 217786697U
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Abstract
The utility model provides a large-volume cell liquid concentration and washing device, which comprises a separation cup and an air bag pipeline; the air bag pipeline is communicated with the separating cup in a mode of being capable of being switched on and switched off; the separation cup performs separation of the cell fluid through high-speed rotation; the air bag pipeline comprises an air bag and an air bag hose, and the air bag is communicated with the separation cup through the air bag hose; the air bag stores the tubing in the device and the air in the separation cup as the cell sap enters the separation cup. The utility model provides a large-volume cell liquid concentration washing device which can be connected with a bag body through a plurality of connection modes such as a puncture mode, a male luer connector, a female luer connector and a sterile connection pipe mode, and is convenient to use; the pressure state in the pipeline can be monitored; through introducing the air bag, make whole device can full-automatic completion cleaning function, need not personnel in the cleaning process and intervene, improved the degree of automation of equipment.
Description
Technical Field
The utility model belongs to biological cell processing field, concretely relates to concentrated washing device of bulky cell liquid.
Background
In the field of biological cell treatment, it is often necessary to concentrate a cultured cell sample solution, remove excess culture medium and waste solution, and then perform subsequent operations such as washing. In a research laboratory environment, cell concentration and washing steps are typically accomplished using a bench top centrifuge, followed by repeated manual pipetting to obtain the final cell suspension. Although manual process operations can also be carried out in GMP-compliant biosafety cabinets, the opening and closing of the container lid carries a high risk of contamination, especially if the operation involves multiple culture containers. Moreover, the manual process has many operation steps, has high technical requirements on operators, depends on the experience and level of the operators, and is not suitable for mass production.
In the prior art, a disposable closed sterile cell sap processing device exists, and can automatically complete the functions of cell sap concentration, washing and the like by matching with corresponding equipment, for example, a cylindrical cup type large-volume cell sap concentration device, and the processing flow thereof is generally as follows: concentrated cell fluid → recovered product fluid → washing residue. However, there are some disadvantages, such as single connector, only fitting the liquid bag of a specific connector, lack of pressure monitoring function, inability to store the tubing and air in the separating cup, resulting in the operator having to hang up the waste bag during the cleaning process and the need to invert the waste bag repeatedly during the cleaning mode.
SUMMERY OF THE UTILITY MODEL
In order to solve one of above-mentioned technical problem at least, the utility model discloses a technical scheme be provide a concentrated washing device of bulky cell liquid, have multiple connected mode, can monitor the pressure in the pipeline and can full-automatic completion cleaning function, need not personnel in the cleaning process and intervene.
In order to realize one of above-mentioned purpose at least, the utility model discloses a technical scheme be:
the utility model provides a large-volume cell liquid concentration and washing device, which comprises a separation cup and an air bag pipeline; the air bag pipeline is communicated with the separating cup in a mode of being capable of being switched on and switched off; the separation cup performs separation of the cell fluid by high-speed rotation; the air bag pipeline comprises an air bag and an air bag hose, and the air bag is communicated with the separation cup through the air bag hose; the air bag stores the tubing in the device and the air in the separation cup as the cell sap enters the separation cup.
Further, the bag type packaging bag also comprises a first bag body connecting pipeline; the first bag body connecting pipeline is communicated with one end of the separating cup; the first bag body connecting pipeline is connected with a sample bag, and cell sap in the sample bag enters the separation cup through the first bag body connecting pipeline.
Further, a second bag body connecting pipeline is also included; the second bag body connecting pipeline is communicated with the other end of the separating cup; the second bag body connecting pipeline is connected with a waste liquid bag, and waste liquid separated by the separating cup is discharged into the waste liquid bag.
Further, first bag body connecting pipeline and second bag body connecting pipeline all include puncture head, two female luer connectors, luer male connector, press and press clamp and hose.
Furthermore, the tail end of the puncture head is a puncture port, and the other end of the puncture head is a male luer connector; both ends of the double female luer connector are female luer connectors; one end of the double female luer connector is connected with the male luer connector end of the puncture head, and the other end of the double female luer connector is connected with the luer male head; the hose is connected with the male luer connector, the pressing clamp is arranged on the hose, and the pressing clamp controls the on-off of the hose.
Further, the device also comprises a product bag pipeline, wherein the product bag pipeline comprises a product bag and a product bag hose; the product bag is communicated with the separation cup through the product bag hose.
Further, the device also comprises a first pressure monitoring pipeline and a pump pipeline; the pump pipeline is connected with the first channel of the separating cup; the other end of the pump pipe pipeline is connected with a first four-way joint; and the three opening ends of the first four-way joint are respectively connected with the first bag body connecting pipeline, the first pressure monitoring pipeline and the product bag pipeline.
Further, the device also comprises a second pressure monitoring pipeline and a separating cup outlet pipeline; the outlet pipeline of the separation cup is connected with the second channel of the separation cup; the other end of the outlet pipeline of the separating cup is connected with a second four-way joint; and the three opening ends of the second four-way joint are respectively connected with the second bag body connecting pipeline, the second pressure monitoring pipeline and the air bag pipeline.
Furthermore, the first pressure monitoring pipeline and the second pressure monitoring pipeline both comprise pressure monitoring heads and connecting hoses; the pressure monitoring head is internally provided with a hydrophobic breathable film, one end of the pressure monitoring head is a conical luer, and the other end of the pressure monitoring head is connected with the connecting hose.
Further, the pump pipe pipeline comprises a first pump clamp, a high-elasticity pump pipe and a second pump clamp; the first pump clamp and the second pump clamp are arranged on the high-elasticity pump pipe at intervals.
The utility model provides a concentrated washing device of bulky cell liquid compares with prior art, the beneficial effects of the utility model reside in that:
in the large-volume cell liquid concentration washing device provided by the utility model, the first bag body connecting pipeline and the second bag body connecting pipeline both comprise puncture heads, double female luer connectors, luer male heads, pressing clamps and hoses; the bag body can be connected with a plurality of connection modes, namely a puncture mode, a male luer connector, a female luer connector and a sterile connection pipe mode, and the bag body is convenient and fast to use.
The utility model provides an among the concentrated washing device of bulky cell liquid, first pressure monitoring pipeline and second pressure monitoring pipeline all are provided with the pressure monitoring head to the pressure state at separation cup both ends can be monitored simultaneously, the pressure condition in the current pipeline can in time be known.
In the large-volume cell liquid concentration washing device provided by the utility model, the whole device can fully automatically complete the washing function by introducing the air bag; the operator does not need to care whether the waste liquid bag is hung up or down, the operator does not need to intervene in the cleaning process, the waste liquid bag is repeatedly inverted, the automation degree of the equipment is improved, and the human participation is reduced.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the scope of the application.
Drawings
FIG. 1 is a schematic structural diagram of a large-volume cellular liquid concentrating and washing device in an embodiment of the present application;
FIG. 2 is a schematic structural view of a bag connecting line in the embodiment of the present application;
the pressure monitoring device comprises a 10 first bag body connecting pipeline, A1A puncture head, A1B double female luer connector, A1C luer male connector, A1D pressing clamp, A1E hose, a 11 second bag body connecting pipeline, a 20 first pressure monitoring pipeline, A2A pressure monitoring head, A2B connecting hose, a 21 second pressure monitoring pipeline, a 3 product bag pipeline, a 3A product bag hose, a 3B product bag, a 40 first four-way connector, a 41 second four-way connector, a 5 pump pipe pipeline, a 5A1 first pump clamp, a 5A2 second pump clamp, a 5B high-elasticity pump pipe, a 6 separating cup, a 6A first channel, a 6B second channel, a 7 separating cup outlet pipeline, an 8 air bag pipeline, an 8A air bag and an 8B air bag hose.
Detailed Description
In order to make those skilled in the art better understand the technical solution of the present invention, the present invention is further described in detail with reference to the specific embodiments. It should be noted that the following described embodiments are exemplary only and are not to be construed as limiting the invention. The examples do not specify particular techniques or conditions, and are performed according to techniques or conditions described in literature in the art or according to the product specification.
In the description of the present invention, it is to be understood that the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implying any number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; the connection can be mechanical connection or electrical connection; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
Hereinafter, the concentrated washing device for large-volume cellular fluid provided by the present invention will be described in detail by specific embodiments:
as shown in fig. 1-2, the present embodiment provides a large-volume cellular fluid concentration washing apparatus, which mainly includes a first bag connecting pipeline 10, a second bag connecting pipeline 11, a first pressure monitoring pipeline 20, a second pressure monitoring pipeline 21, a product bag pipeline 3, a first four-way joint 40, a second four-way joint 41, a pump pipeline 5, a separating cup 6, a separating cup outlet pipeline 7, and an air bag pipeline 8.
The first bag body connecting pipeline 10 and the second bag body connecting pipeline 11 have the same structure and respectively comprise a puncture head 1A, a double female luer connector 1B, a luer male connector 1C, a pressing clamp 1D and a hose 1E. The tail end of the puncture head 1A is a puncture port, and the other end of the puncture head is a male luer connector; both ends of the double female luer connector 1B are female luer connectors; one end of the double female luer connector 1B is connected with the male luer connector end of the puncture head 1A, and the other end of the double female luer connector 1B is connected with the luer male connector 1C; the hose 1E is connected with the male luer 1C, the pressing clip 1D is arranged on the hose 1E, the pressing clip 1D is operated to control the on/off of the hose 1E.
The above-described structure of the first pouch connecting line 10 and the second pouch connecting line 11 enables various connection methods, i.e., a piercing method, a male luer, a female luer, and a sterile connection tube, to be connected to the pouch.
The first pressure monitoring pipeline 20 and the second pressure monitoring pipeline 21 have the same structure and respectively comprise a pressure monitoring head 2A and a connecting hose 2B. A hydrophobic breathable film is arranged in the pressure monitoring head 2A, one end of the pressure monitoring head 2A is a tapered Ruhr opening, and the other end is connected with the connecting hose 2B.
The product bag conduit 3 includes a product bag 3B and a product bag hose 3A. The product bag 3B is connected to a product bag hose 3A.
The first bag body connecting pipeline 10, the first pressure monitoring pipeline 20 and the product bag pipeline 3 are connected to the three opening ends of the first four-way joint 40 in parallel, and the other end of the first four-way joint 40 is connected with the pump pipe pipeline 5.
The pump pipe 5 includes a first pump clamp 5A1, a highly elastic pump pipe 5B, and a second pump clamp 5A2. The two pump clamps are adhered to the high-elasticity pump pipe 5B and are arranged at intervals to be matched with a peristaltic pump (not shown in the figure) for use, and the peristaltic pump provides power for transferring the cell sap in the large-volume cell sap concentration washing device.
The separation cup 6 is a cone-shaped separation cup, and when the separation cup 6 rotates at a high speed, cells in the separation cup can be separated from waste liquid. The separation cup 6 comprises a first channel 6A and a second channel 6B; the first channel 6A is connected with the pump pipe pipeline 5, the second channel 6B is connected with the separation cup outlet pipeline 7, the other end of the separation cup outlet pipeline 7 is connected with the second four-way connector 41, and three opening ends of the second four-way connector 41 are respectively connected with the second bag body connecting pipeline 11, the second pressure monitoring pipeline 21 and the air bag pipeline 8. The air bag pipeline 8 comprises an air bag 8A and an air bag hose 8B, the air bag 8A can store air in the pipeline and the separating cup in the whole device during liquid inlet, and air is supplied during liquid outlet so as to prevent negative pressure from being formed.
The specific working principle of the large-volume cellular fluid concentration washing device provided in this embodiment is described as follows:
a first bag body connecting pipeline 10 is connected with a sample bag, a second bag body connecting pipeline 11 is connected with a waste liquid bag, and the device is connected with a power device;
under the action of a peristaltic pump in the power device, sample liquid enters a separation cup 6 rotating at a high speed through a first bag body connecting pipeline 10, a first four-way joint 40 and a pump pipe pipeline 5 for separation; meanwhile, the air in the pipeline and the separating cup enters the air bag 8A for storage;
along with the continuous entering of the sample liquid, the cells in the separation cup 6 can be left in the separation cup 6, when the separation cup 6 is filled with the sample liquid, the valve in the power device cuts off the air bag pipeline 8, and simultaneously the second bag body connecting pipeline 11 is opened, so that the waste liquid can enter the waste liquid bag;
when the sample liquid is concentrated, the valve in the power device disconnects the second bag body connecting pipeline 11, the air bag pipeline 8 is opened, the peristaltic pump in the power device rotates reversely, and cells in the separation cup 6 are pumped back into the product bag 3B;
since the separation cup 6 cannot completely empty the remaining cell sap in the cup and the concentration of the remaining cell sap is very high, a small amount of waste liquid needs to be pumped back to clean the separation cup 6. At the moment, the air bag pipeline 8 is closed, the second bag connecting pipeline 11 is opened, the peristaltic pump rotates reversely, a preset amount of waste liquid is pumped into the separation cup 6, then the second bag connecting pipeline 11 is closed, the air bag pipeline 8 is opened, the peristaltic pump rotates reversely continuously, and all liquid in the separation cup 6, the pump pipe pipeline 5, the first four-way joint 40 and the product bag pipeline 3 can be emptied into the product bag 3B through air supplement, so that the cell liquid concentration and washing operation is completed.
In the present application, unless expressly stated or limited otherwise, the first feature may be directly on or directly under the second feature or indirectly via intermediate members. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.
Claims (6)
1. A large-volume cell liquid concentration washing device is characterized by comprising a separation cup and an air bag pipeline; the air bag pipeline is communicated with the separating cup in an on-off mode; the separation cup performs separation of the cell fluid by high-speed rotation; the air bag pipeline comprises an air bag and an air bag hose, and the air bag is communicated with the separation cup through the air bag hose; the air bag stores the pipeline in the device and the air in the separation cup when the cell sap enters the separation cup; the washing device also comprises a first bag body connecting pipeline and a second bag body connecting pipeline; the first bag body connecting pipeline is communicated with one end of the separating cup; the first bag body connecting pipeline is connected with a sample bag, and cell sap in the sample bag enters the separation cup through the first bag body connecting pipeline; the second bag body connecting pipeline is communicated with the other end of the separating cup; the second bag body connecting pipeline is connected with a waste liquid bag, and waste liquid separated by the separating cup is discharged into the waste liquid bag; the first bag body connecting pipeline and the second bag body connecting pipeline respectively comprise a puncture head, a double female luer connector, a luer male head, a pressing clamp and a hose; the tail end of the puncture head is a puncture opening, and the other end of the puncture head is a male luer connector; both ends of the double female luer connector are female luer connectors; one end of the double female luer connector is connected with the male luer connector end of the puncture head, and the other end of the double female luer connector is connected with the luer male head; the hose is connected with the male luer connector, the pressing clamp is arranged on the hose, and the pressing clamp controls the on-off of the hose.
2. The large volume cellular liquid concentrated washing device of claim 1, further comprising a product bag conduit comprising a product bag and a product bag hose; the product bag is in communication with the separation cup via the product bag hose.
3. The large volume cellular liquid concentrated washing device according to claim 2, further comprising a first pressure monitoring line and a pump line; the pump pipeline is connected with the first channel of the separating cup; the other end of the pump pipe pipeline is connected with a first four-way joint; and the three opening ends of the first four-way joint are respectively connected with the first bag body connecting pipeline, the first pressure monitoring pipeline and the product bag pipeline.
4. The large volume cellular liquid concentrated washing device according to claim 3, further comprising a second pressure monitoring line and a separator cup outlet line; the outlet pipeline of the separation cup is connected with the second channel of the separation cup; the other end of the outlet pipeline of the separating cup is connected with a second four-way joint; and the three opening ends of the second four-way joint are respectively connected with the second bag body connecting pipeline, the second pressure monitoring pipeline and the air bag pipeline.
5. The large volume cellular liquid concentrating and washing device according to claim 4, wherein the first pressure monitoring line and the second pressure monitoring line each comprise a pressure monitoring head and a connection hose; the pressure monitoring head is internally provided with a hydrophobic breathable film, one end of the pressure monitoring head is a conical luer, and the other end of the pressure monitoring head is connected with the connecting hose.
6. The concentrated washing device for large volume cellular liquid according to claim 5, wherein the pump pipe line comprises a first pump clamp, a highly elastic pump pipe and a second pump clamp; the first pump clamp and the second pump clamp are arranged on the high-elasticity pump pipe at intervals.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220111922.XU CN217786697U (en) | 2022-01-17 | 2022-01-17 | Concentrated washing device of bulky cellular liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220111922.XU CN217786697U (en) | 2022-01-17 | 2022-01-17 | Concentrated washing device of bulky cellular liquid |
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| Publication Number | Publication Date |
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| CN217786697U true CN217786697U (en) | 2022-11-11 |
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| CN202220111922.XU Active CN217786697U (en) | 2022-01-17 | 2022-01-17 | Concentrated washing device of bulky cellular liquid |
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