CN215712960U - Quantitative, aseptic and disposable automatic cell sampling device - Google Patents
Quantitative, aseptic and disposable automatic cell sampling device Download PDFInfo
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- CN215712960U CN215712960U CN202121581964.1U CN202121581964U CN215712960U CN 215712960 U CN215712960 U CN 215712960U CN 202121581964 U CN202121581964 U CN 202121581964U CN 215712960 U CN215712960 U CN 215712960U
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Abstract
The utility model relates to a quantitative, sterile and disposable automatic cell sampling device, which comprises a sampling container, a pump, a sterile filter, a control valve, a first conduit and a second conduit; the sampling container is internally provided with an accommodating cavity, and the top of the sampling container is provided with a first interface and a second interface which are used for realizing the maximum sampling capacity of the sampling container; one end of the first conduit is connected with the first interface, the other end of the first conduit is connected with the cell medicine production device through a pump, and the middle part of the first conduit is provided with a control valve; the second conduit is connected between the second interface and the sterile filter and is used for exhausting and supplementing sterile air in the sampling container; the sterile filter is used to filter air to sterile air. The automatic cell sampling device has the characteristics of small volume, high space utilization rate of the sampling container, capability of sampling aseptically, great reduction of the cell pollution probability and convenience in sampling.
Description
Technical Field
The utility model relates to the technical field of medicine sampling, in particular to a quantitative, sterile and disposable automatic cell sampling device.
Background
The production process of cell medicines has the characteristics of multiple preparation links and complex process, so that the complex process operation and the cell in vitro environment have obvious influence on the cell bioactivity; therefore, cell sampling is required periodically during the production process of cell-based drugs to monitor the cell's activity, loss rate and density. When the existing sampling device is used for sampling, an experimenter usually uses the sterile sampling device for sampling, and the existing sterile sampling method generally moves equipment into a sterile environment and opens a cover for sampling.
However, the sampling device of the prior art is cumbersome to operate, and frequent opening of the lid is prone to contamination. In addition, if the container is large in size, the area of carrying and a sterile environment is required to be higher, so that the sampling device in the prior art has the defects of inconvenience in sampling, easiness in pollution and the like.
SUMMERY OF THE UTILITY MODEL
The utility model provides a quantitative, sterile and disposable automatic cell sampling device, which has the characteristics of small volume, high space utilization rate of a sampling container, capability of sampling aseptically, great reduction of the cell pollution probability and convenience in sampling.
In order to achieve the purpose, the utility model adopts the following specific technical scheme:
the utility model provides a quantitative, sterile and disposable automatic cell sampling device, which comprises a sampling container, a pump, a sterile filter, a control valve, a first conduit and a second conduit;
the sampling container is internally provided with a containing cavity for containing samples, and the top of the sampling container is provided with a first interface and a second interface which are communicated with the containing cavity and are used for realizing the maximum sampling capacity of the sampling container;
one end of the first conduit is connected with the first interface, the other end of the first conduit is connected with a cell drug production device through the pump, and the control valve for controlling on-off is arranged in the middle of the first conduit;
the second conduit is connected between the second interface and the sterile filter and is used for discharging and supplementing sterile air in the sampling container;
the sterile filter is used for filtering the passing air into sterile air;
the control valve has an opening state and a closing state, and when the control valve is in the opening state, two ends of the first conduit are in a communication state; when the control valve is in a closed state, two ends of the first conduit are in a cut-off state.
Still further, the pump is a peristaltic pump.
Further, the sampling container is a transparent container provided with scales.
Further, the control valve is a pipe clamp, a solenoid valve, an electric valve, a pneumatic valve or a hydraulic valve.
Still further, the sampling container is a container made of a flexible material.
In addition, the present invention provides a cell sampling method using the above automated cell sampling apparatus, which introduces a cell fluid into a sampling vessel by generating a fluid driving force by a pump, the cell sampling method comprising the steps of:
closing a control valve of the automatic cell sampling device to enable the control valve to be in a closed state, communicating a pump of the automatic cell sampling device with a cell medicine production device, and enabling a first interface and a second interface to be located at the highest position of a sampling container;
starting a control valve and a pump of the automatic cell sampling device, and quantitatively conveying the cell sap of the cell medicine production device to a containing cavity of a sampling container through the pump;
and closing a control valve and a pump of the automatic cell sampling device, and sealing a first conduit between the sampling container and the control valve to realize quantitative, sterile and disposable cell sampling.
Further, between turning on the control valve and the pump of the automated cell sampling apparatus and turning off the control valve and the pump of the automated cell sampling apparatus, the steps of:
controlling the pump to rotate reversely, leading the air in the sampling container and the air filtered by the sterile filter into the first conduit, and leading the cell sap in the first conduit to flow back to the cell medicine production device.
Further, when the first conduit between the sampling container and the control valve is subjected to the sealing treatment, the first conduit is subjected to the heat-melting sealing treatment by using the heat-melting device.
Further, after the sealing process of the first conduit between the sampling container and the control valve, the method further comprises the following steps:
and connecting another automatic cell sampling device at the first catheter in a sealing way in a sterile manner, and repeating the steps to realize multiple times of sterile sampling.
Further, in aseptically connecting another automated cell sampling apparatus at the first tube of the sealing process, the first tube of the another automated cell sampling apparatus is connected to the first tube of the sealing process through a sterile tube connector.
Compared with the prior art, the utility model has the following beneficial effects:
the sampling container of the automatic cell sampling device is connected with the cell medicine production device through the pump, and the cell fluid in the cell medicine production device is guided into the sampling container by the power source provided by the pump, so that the quantitative, aseptic and disposable cell sampling function can be realized; the first interface and the second interface of the sampling container are arranged at the top, so that the maximum sampling capacity of cell sap in the sampling container can be realized, and the maximum utilization efficiency can be obtained under the condition of small volume; the control valve is arranged in the middle of the first conduit connecting the pump and the sampling container, and the opening and closing of the sampling channel can be conveniently controlled through the control valve, so that the sampling is easy and convenient; in addition, the sampling container is connected with the sterile filter, the air entering the sampling container is filtered through the sterile filter, in order to avoid the waste of the cell sap in the first conduit, the pump can be operated reversely, namely, the air in the sampling container and/or the sterile air filtered by the sterile filter can be introduced into the first conduit, so that the cell sap in the first conduit can flow back to the cell medicine production device, and the sterile air is provided for the sampling container due to the fact that a pollution source in a working environment can be filtered through the sterile filter, and the pollution of the cell sap is avoided; therefore, the automatic cell sampling device has the characteristics of small volume, high space utilization rate of the sampling container, capability of sampling aseptically, great reduction of the cell pollution probability and convenience in sampling.
In addition, by adopting the automatic cell sampling device and the cell sampling method, the function of aseptic sampling for a plurality of times can be realized by connecting a plurality of automatic cell sampling devices in the cell medicine production device; and a plurality of interfaces can be arranged at the first conduit to be connected with a plurality of automatic cell sampling devices or to be repeatedly connected with a plurality of automatic cell sampling devices through the sterile pipe connecting machine, and the function of multiple sterile sampling can be realized.
Drawings
FIG. 1 is a schematic diagram of an automated cell sampling apparatus according to the present invention;
FIG. 2 is another schematic diagram of the automated cell sampling apparatus of the present invention;
FIG. 3 is a process flow diagram of the cell sampling method of the present invention.
Wherein, 1-a sampling container, 2-a pump, 3-a sterile filter, 4-a control valve, 5-a first conduit, 6-a second conduit, 11-a first interface, 12-a second interface
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example one
As shown in fig. 1 and 2, the present embodiment provides a quantitative, sterile, disposable automated cell sampling device, which includes a sampling vessel 1, a pump 2, a sterile filter 3, a control valve 4, a first conduit 5, and a second conduit 6;
the sampling container 1 has a containing cavity (not shown) for containing cell sap and other samples inside, and the top of the sampling container is provided with a first interface 11 and a second interface 12 which are communicated with the containing cavity and are used for realizing the maximum sampling capacity of the sampling container 1; as shown in the structure of fig. 1, the first interface 11 is disposed at the top of the left side of the sampling container 1, the second interface 12 is disposed at the top of the right side of the sampling container 1, and the first interface 11 and the second interface 12 are disposed oppositely; as shown in the structure of fig. 2, the first interface 11 is disposed at the left side of the top of the sampling container 1, and the second interface 12 is disposed at the right side of the top of the sampling container 1; wherein, when sampling, the first interface 11 is used as a cell fluid inlet, and the second interface 12 is used as a gas outlet; when the cell fluid in the first conduit 5 is returned to the cell-based drug production device, the first port 11 is used as a sterile gas outlet, and the second port 12 is used as a sterile gas inlet after being filtered by the sterile filter 3; the sampling container 1 can be made of flexible materials such as plastic or rubber; when the sampling container 1 is extruded, the air in the sampling container 1 is guided to the cell medicine production device; when the sampling container 1 is loosened after extrusion, the negative pressure in the sampling container 1 can suck the cell sap in the cell medicine production device into the sampling container 1, and at the moment, sterile sampling can be realized without the work of the pump 2; the sampling container 1 may also be a rigid container made of plastic or glass;
the pump 2 is used for driving cell sap to flow; the pump 2 may be a peristaltic pump; the pump 2 is used for providing a power source for sampling, so that cell sap enters the sampling container 1 from the cell medicine production device;
one end of the first conduit 5 is connected with the first interface 11, and the other end is connected with the cell drug production device through the pump 2, and the middle part of the first conduit 5 is provided with a control valve 4 for controlling on-off; the first conduit 5 introduces the cell fluid driven by the pump 2 into the sampling vessel 1; the control valve 4 is used for controlling the on-off of the first conduit 5 so as to control whether the cell liquid can enter the sampling container 1 through the first conduit 5 or flow back to the cell medicine production device;
a second conduit 6 is connected between the second interface 12 and the sterile filter 3 for the evacuation and replenishment of sterile air inside the sampling container 1; a second conduit 6 is connected between the sampling container 1 and the sterile filter 3, and when sampling, sterile air in the sampling container 1 can be discharged through the sterile filter 3; when the cellular fluid is returned to the cell-based drug production apparatus in order to avoid waste of the cellular fluid, the second conduit 6 is used to introduce the sterile air filtered by the sterile filter 3 into the sampling vessel 1;
the sterile filter 3 is used for filtering the passing air into sterile air, and the sterile filter 3 can carry out sterile filtration on the air so as to prevent external pollution sources from entering the sampling container 1 and even the cell medicine production device through the sterile filter 3; the sterile filter 3 may be a lidded sterile filter 3; the sterile filter 3 may be provided with a lid;
the control valve 4 has an open state and a closed state, and when the control valve 4 is in the open state, both ends of the first conduit 5 are in a communication state; when the control valve 4 is in a closed state, two ends of the first conduit 5 are in a cut-off state; the control valve 4 can be a manual control valve 4, and can also be an automatic control valve 4, such as: a pipe clamp, a solenoid valve, an electrically operated valve, a pneumatic valve or a hydraulically operated valve.
The sampling container 1 of the automatic cell sampling device is connected with the cell medicine production device through the pump 2, and the cell fluid in the cell medicine production device is guided into the sampling container 1 through the power source provided by the pump 2, so that the quantitative, aseptic and disposable cell sampling function can be realized; the first interface 11 and the second interface 12 are arranged at the top of the sampling container 1, so that the maximum sampling capacity of the cell sap in the sampling container 1 can be realized, the maximum cell sap capacity can be obtained when the sampling container 1 is in a small volume, and the utilization efficiency of the sampling container 1 is improved; the control valve 4 is arranged in the middle of the first conduit 5 connecting the pump 2 and the sampling container 1, and the opening and closing of the sampling channel can be conveniently controlled through the control valve 4, so that the cell sampling becomes easy and convenient.
Because the sampling container 1 is connected with the sterile filter 3 through the second conduit 6, the air entering the sampling container 1 can be filtered through the sterile filter 3, and a pollution source is prevented from entering the sampling container 1 and even a cell medicine production device; in order to avoid the waste of cell sap in the first guide tube 5, the pump 2 may be operated in reverse, i.e., air in the sampling container 1 and/or sterile air filtered by the sterile filter 3 may be introduced into the first guide tube 5, so that the cell sap in the first guide tube 5 may be returned to the cell-based drug manufacturing apparatus, and since the contamination source in the working environment may be filtered by the sterile filter 3, the sampling container 1 is supplied with sterile air, thereby preventing the contamination of the cell sap.
Therefore, above-mentioned automatic cell sampling device has small, sample container 1 space utilization is high, can aseptic sample, greatly reduced cell by contaminated probability and sample convenient characteristics.
In order to facilitate accurate control of the sample volume, the sampling vessel 1 may be a transparent vessel provided with graduations. The sampling amount of the cell sap can be visually observed through the transparent container, and meanwhile, the accurate sampling amount of the cell sap can be directly obtained by combining scales.
Example two
The embodiment of the utility model provides a cell sampling method adopting the automatic cell sampling device, which uses a pump 2 to generate fluid driving force to introduce cell fluid into a sampling container 1, and the cell sampling method comprises the following steps:
step S10, closing the control valve 4 of the automated cell sampling device to make it in a closed state, communicating the first conduit 5 of the automated cell sampling device with the cell drug production device, and making the first interface 11 and the second interface 12 both in the highest position of the sampling container 1;
step S20, opening the control valve 4 and the pump 2 of the automatic cell sampling device, and quantitatively conveying the cell sap of the cell drug production device to the accommodating cavity of the sampling container 1 through the pump 2; starting a control valve 4 and a pump 2 of the automatic cell sampling device, namely, enabling the control valve 4 to enter an opening state and enabling the pump 2 to enter a working state;
and step S40, closing the control valve 4 and the pump 2 of the automatic cell sampling device, and sealing the first conduit 5 between the sampling container 1 and the control valve 4 to realize quantitative, sterile and disposable cell sampling. The control valve 4 and the pump 2 of the automated cell sampling apparatus are closed, that is, the control valve 4 is brought into a closed state, and the pump 2 is stopped.
The cell sampling method can provide a sampling power source through the pump, so that the cell can be conveniently and quickly sampled from the cell drug production device, and the quantitative, aseptic and disposable cell sampling function can be realized.
As shown in FIG. 3, the embodiment of the present invention also provides a cell sampling method using the above-mentioned automated cell sampling apparatus, which introduces cell fluid into a sampling vessel 1 by a fluid driving force generated by a pump 2, the cell sampling method comprising the steps of:
step S10, closing the control valve 4 of the automated cell sampling device to make it in a closed state, communicating the first conduit 5 of the automated cell sampling device with the cell drug production device, and making the first interface 11 and the second interface 12 both in the highest position of the sampling container 1;
step S20, opening the control valve 4 and the pump 2 of the automatic cell sampling device, and quantitatively conveying the cell sap of the cell drug production device to the accommodating cavity of the sampling container 1 through the pump 2; starting a control valve 4 and a pump 2 of the automatic cell sampling device, namely, enabling the control valve 4 to enter an opening state and enabling the pump 2 to enter a working state;
step S30, controlling the pump 2 to rotate reversely, leading the air in the sampling container 1 and the air filtered by the sterile filter 3 into the first conduit 5, and leading the cell sap in the first conduit 5 to flow back to the cell medicine production device;
and step S40, closing the control valve 4 and the pump 2 of the automatic cell sampling device, and sealing the first conduit 5 between the sampling container 1 and the control valve 4 to realize quantitative, sterile and disposable cell sampling. The control valve 4 and the pump 2 of the automated cell sampling apparatus are closed, that is, the control valve 4 is brought into a closed state, and the pump 2 is stopped.
The cell sampling method can provide a sampling power source through the pump, can conveniently and quickly sample from the cell medicine production device, and realizes the quantitative, sterile and disposable cell sampling function; meanwhile, the cell sap in the first conduit 5 can be returned to the cell drug production device in an aseptic state through the reverse rotation of the pump 2, so that the waste of the cell sap in the first conduit 5 is avoided.
As shown in fig. 3, in order to realize multiple aseptic sampling, the cell sampling method according to the embodiment of the present invention may further include the following steps after the first conduit 5 between the sampling container 1 and the control valve 4 is sealed, on the basis of the above-mentioned various cell sampling methods:
step S50, connecting another automatic cell sampling device at the first conduit 5 for sealing treatment in a sterile way, and repeating the steps to realize multiple times of sterile sampling; that is, after the cell sap sampling is completed once by using one automated cell sampling apparatus, another sterile cell sampling apparatus may be continuously connected to the first tube 5 which is fused and sealed, and the above steps S10, S20 and S40 may be repeatedly completed, or the above steps S10, S20, S30 and S40 may be repeatedly completed, thereby completing the cell sap sampling for the second time; repeated operation can also realize the cell sap sampling function for many times.
Through aseptic connection of another automatic cell sampling device at the first catheter 5 which is sealed, aseptic sampling for the second time can be realized by circulating the steps S10-S40, and the steps S50, S10, S20 and S40 are circularly executed, or the steps S50, S10, S20, S30 and S40 are circularly executed, so that aseptic sampling for multiple times can be realized conveniently and quickly under the condition of avoiding pollution, and the flexibility of aseptic sampling is improved.
To achieve sterile connection of the further automated cell sampling apparatus at the first conduit 5 of the sealing process, the first conduit 5 of the further automated cell sampling apparatus may be connected into the first conduit 5 of the sealing process by means of a sterile tube connector.
On the basis of the cell sampling method, the first conduit 5 between the sampling container 1 and the control valve 4 is subjected to hot melting sealing treatment by adopting a hot melting device, so that the disposable cell sampling of the cell sap can be conveniently and quickly realized.
It will be apparent to those skilled in the art that various changes and modifications may be made in the embodiments of the present invention without departing from the spirit and scope of the utility model. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (5)
1. A quantitative, sterile, disposable, automated cell sampling device comprising a sampling container, a pump, a sterile filter, a control valve, a first conduit, and a second conduit;
the sampling container is internally provided with a containing cavity for containing samples, and the top of the sampling container is provided with a first interface and a second interface which are communicated with the containing cavity and are used for realizing the maximum sampling capacity of the sampling container;
one end of the first conduit is connected with the first interface, the other end of the first conduit is connected with a cell drug production device through the pump, and the control valve for controlling on-off is arranged in the middle of the first conduit;
the second conduit is connected between the second interface and the sterile filter and is used for discharging and supplementing sterile air in the sampling container;
the sterile filter is used for filtering the passing air into sterile air;
the control valve has an opening state and a closing state, and when the control valve is in the opening state, two ends of the first conduit are in a communication state; when the control valve is in a closed state, two ends of the first conduit are in a cut-off state.
2. The automated cell sampling device of claim 1, wherein the pump is a peristaltic pump.
3. The automated cell sampling device of claim 1, wherein the sampling container is a transparent container provided with a scale.
4. The automated cell sampling device of any one of claims 1-3, wherein the control valve is a tube clamp, a solenoid valve, an electrically operated valve, a pneumatically operated valve, or a hydraulically operated valve.
5. The automated cell sampling device of any one of claims 1-3, wherein the sampling container is a container made of a flexible material.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202121581964.1U CN215712960U (en) | 2021-07-13 | 2021-07-13 | Quantitative, aseptic and disposable automatic cell sampling device |
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| CN202121581964.1U CN215712960U (en) | 2021-07-13 | 2021-07-13 | Quantitative, aseptic and disposable automatic cell sampling device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113528302A (en) * | 2021-07-13 | 2021-10-22 | 深圳赛桥生物创新技术有限公司 | Quantitative, sterile and disposable automatic cell sampling device and method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113528302A (en) * | 2021-07-13 | 2021-10-22 | 深圳赛桥生物创新技术有限公司 | Quantitative, sterile and disposable automatic cell sampling device and method |
| WO2023284580A1 (en) * | 2021-07-13 | 2023-01-19 | 深圳赛桥生物创新技术有限公司 | Quantitative, sterile and disposable automatic cell sampling device and method |
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